AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003934

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003934.t1 MTR_4g065870 90.554 307 13 4 45 343 1 299 2.13e-179 506
MS.gene003934.t1 MTR_4g065870 90.554 307 13 4 45 343 1 299 3.16e-179 504
MS.gene003934.t1 MTR_4g065870 90.554 307 13 4 45 343 1 299 1.93e-178 503
MS.gene003934.t1 MTR_2g098310 42.901 324 114 12 45 342 1 279 1.80e-65 214
MS.gene003934.t1 MTR_2g098310 42.901 324 114 12 45 342 1 279 2.02e-65 212
MS.gene003934.t1 MTR_2g098310 42.901 324 114 12 45 342 1 279 2.82e-65 213
MS.gene003934.t1 MTR_2g098310 42.901 324 114 12 45 342 1 279 2.85e-65 214
MS.gene003934.t1 MTR_2g098310 42.901 324 114 12 45 342 1 279 2.96e-65 213
MS.gene003934.t1 MTR_1g052470 33.333 129 75 4 225 345 127 252 6.88e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003934.t1 AT1G51140 32.143 224 112 8 45 263 1 189 1.69e-17 83.6

Find 63 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTAATAATTTGAGTAATTA+TGG 0.186951 4.2:-39819012 MS.gene003934:CDS
ACCCTTTCTGCGATGCTTCT+TGG 0.204939 4.2:+39817372 None:intergenic
CATCAACAACAAATGAATTC+AGG 0.258063 4.2:-39819399 MS.gene003934:CDS
CCCTTTCTGCGATGCTTCTT+GGG 0.281243 4.2:+39817373 None:intergenic
TCAACTACTAAATCAGTTAT+AGG 0.300998 4.2:+39819174 None:intergenic
CGGGTTTCAGCAAAACCTTC+AGG 0.325691 4.2:+39817954 None:intergenic
TCAAACACTTATTCATGCTT+TGG 0.334130 4.2:-39817258 MS.gene003934:CDS
ATGATATCTGATAATATTAC+TGG 0.350123 4.2:-39817876 MS.gene003934:CDS
CCTAACGGTGAGGCATAGTT+TGG 0.353263 4.2:+39818020 None:intergenic
CTTTCTGTTTCAGGACTTGA+AGG 0.354030 4.2:+39819294 None:intergenic
TTTGCTGCTATGAGAGGAAT+AGG 0.354357 4.2:-39818116 MS.gene003934:CDS
TATGAGAGGAATAGGAACTA+TGG 0.359228 4.2:-39818108 MS.gene003934:CDS
GGTGGTAGTGGTGGTGGTGG+TGG 0.363507 4.2:-39819234 MS.gene003934:CDS
TCAAACATTAACATTGATAC+TGG 0.367647 4.2:-39818871 MS.gene003934:intron
TCAACCTCTTCCTTAACTAT+AGG 0.381573 4.2:+39819108 None:intergenic
TTTATCCCGGGTTTCCCCTT+AGG 0.384096 4.2:-39817921 MS.gene003934:CDS
GGAAGTGGTGGTAGTGGTGG+TGG 0.399180 4.2:-39819240 MS.gene003934:CDS
TCACCGTTAGGAGCTGAAAT+CGG 0.415027 4.2:-39818008 MS.gene003934:CDS
CCCAAGAAGCATCGCAGAAA+GGG 0.421337 4.2:-39817373 MS.gene003934:intron
AAGGTGGTCTTCCTGAATTA+TGG 0.439700 4.2:+39818962 None:intergenic
CGACCGGTCTTCATTTGATT+CGG 0.440686 4.2:+39818937 None:intergenic
GCAACAGCTTCCTATAGTTA+AGG 0.446191 4.2:-39819118 MS.gene003934:CDS
AGTGGTGGTAGTGGTGGTGG+TGG 0.447971 4.2:-39819237 MS.gene003934:CDS
TGATGATCTAGAAACATTGT+TGG 0.448634 4.2:+39819429 None:intergenic
CACCGTTAGGAGCTGAAATC+GGG 0.453811 4.2:-39818007 MS.gene003934:CDS
ATCCTCCCATGTAGTACCTA+AGG 0.461462 4.2:+39817905 None:intergenic
CCAAACTATGCCTCACCGTT+AGG 0.468050 4.2:-39818020 MS.gene003934:CDS
ATGAATAGTCTTAGTGGAAG+TGG 0.476450 4.2:-39819255 MS.gene003934:CDS
ATCAACAACAAATGAATTCA+GGG 0.477414 4.2:-39819398 MS.gene003934:CDS
GTCTTCATTTGATTCGGTAA+AGG 0.477771 4.2:+39818943 None:intergenic
GTTTCCCCTTAGGTACTACA+TGG 0.505709 4.2:-39817911 MS.gene003934:CDS
AGTGGAAGTGGTGGTAGTGG+TGG 0.511352 4.2:-39819243 MS.gene003934:CDS
CTTAGTGGAAGTGGTGGTAG+TGG 0.518560 4.2:-39819246 MS.gene003934:CDS
TGCGATGCTTCTTGGGTGAG+TGG 0.520586 4.2:+39817380 None:intergenic
AGGTTTATGAATAGTCTTAG+TGG 0.525192 4.2:-39819261 MS.gene003934:CDS
AGACATGGCAGTTCACCTGC+TGG 0.529643 4.2:-39818901 MS.gene003934:CDS
ACCCAAGAAGCATCGCAGAA+AGG 0.530358 4.2:-39817374 MS.gene003934:CDS
TTCATTTGATTCGGTAAAGG+TGG 0.540846 4.2:+39818946 None:intergenic
GCTTCCTATAGTTAAGGAAG+AGG 0.542479 4.2:-39819112 MS.gene003934:CDS
ATGAGAGGAATAGGAACTAT+GGG 0.549779 4.2:-39818107 MS.gene003934:CDS
ATGTTTGAAAACAATCCAGC+AGG 0.553542 4.2:+39818886 None:intergenic
CTCCCGATTTCAGCTCCTAA+CGG 0.555564 4.2:+39818005 None:intergenic
TCCTCCCATGTAGTACCTAA+GGG 0.558794 4.2:+39817906 None:intergenic
CGGGATAAAATCGTTACCCC+GGG 0.559844 4.2:+39817935 None:intergenic
CAAAACAATCTTGAACCTGA+AGG 0.566426 4.2:-39817969 MS.gene003934:CDS
CGGTCTAGTCTTATTAGACA+TGG 0.571581 4.2:-39818916 MS.gene003934:CDS
GTAGTACCTAAGGGGAAACC+CGG 0.587146 4.2:+39817915 None:intergenic
AAATGAATTCAGGGTTAACA+CGG 0.591208 4.2:-39819389 MS.gene003934:CDS
TGGCACAGCCTCGTTTAGCA+CGG 0.591219 4.2:+39817400 None:intergenic
TGCAAAATCCGTGCTAAACG+AGG 0.592385 4.2:-39817408 MS.gene003934:CDS
CCGGGATAAAATCGTTACCC+CGG 0.596209 4.2:+39817934 None:intergenic
AATGAATAACAATGAATCTG+TGG 0.596436 4.2:-39819061 MS.gene003934:CDS
CCCCTTAGGTACTACATGGG+AGG 0.597480 4.2:-39817907 MS.gene003934:CDS
TCTAATAAGACTAGACCGAC+CGG 0.616887 4.2:+39818921 None:intergenic
AATAGTCTTAGTGGAAGTGG+TGG 0.627049 4.2:-39819252 MS.gene003934:CDS
TTACCGAATCAAATGAAGAC+CGG 0.652711 4.2:-39818940 MS.gene003934:CDS
CGAATCAAATGAAGACCGGT+CGG 0.664050 4.2:-39818936 MS.gene003934:CDS
CCTCCCATGTAGTACCTAAG+GGG 0.665348 4.2:+39817907 None:intergenic
TTTCCCCTTAGGTACTACAT+GGG 0.669210 4.2:-39817910 MS.gene003934:CDS
GCGTGTAGTGAAGAGAGTGG+TGG 0.682382 4.2:+39820024 None:intergenic
TAGTACCTAAGGGGAAACCC+GGG 0.682720 4.2:+39817916 None:intergenic
GTAGCGTGTAGTGAAGAGAG+TGG 0.703050 4.2:+39820021 None:intergenic
GATTTCAGCTCCTAACGGTG+AGG 0.706801 4.2:+39818010 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAATAATTTGAGTAATTA+TGG - chr4.2:39818290-39818309 MS.gene003934:intron 15.0%
!! ATATTTGAAAAATATGAACT+TGG + chr4.2:39817951-39817970 None:intergenic 15.0%
!! AAGTTAATTAGAAATCCAAT+AGG - chr4.2:39819084-39819103 MS.gene003934:CDS 20.0%
!! ATGATATCTGATAATATTAC+TGG - chr4.2:39819426-39819445 MS.gene003934:CDS 20.0%
!!! ACTAGAGGTATTTTTTTTTT+TGG - chr4.2:39817348-39817367 MS.gene003934:intron 20.0%
!!! ATTTACATTTTGTGCATATT+TGG + chr4.2:39818765-39818784 None:intergenic 20.0%
!!! GATCGAATTTTTATTTTTAC+AGG - chr4.2:39817797-39817816 MS.gene003934:intron 20.0%
!!! TCATTTTCTTGATCATAAAT+AGG - chr4.2:39819127-39819146 MS.gene003934:CDS 20.0%
!!! TGTAGTTTTTATCAAAATCA+CGG - chr4.2:39818704-39818723 MS.gene003934:intron 20.0%
! AAAACTGAAAAAGATGAAAG+AGG + chr4.2:39817677-39817696 None:intergenic 25.0%
! AAACAACAAAGACAATAGTT+TGG + chr4.2:39817521-39817540 None:intergenic 25.0%
! AAACTGAAAAAGATGAAAGA+GGG + chr4.2:39817676-39817695 None:intergenic 25.0%
! AATGAATAACAATGAATCTG+TGG - chr4.2:39818241-39818260 MS.gene003934:intron 25.0%
! ACCATGTAAAACAAAGAAAA+AGG + chr4.2:39818570-39818589 None:intergenic 25.0%
! ATAACAAAGTAAAGCTACTT+AGG + chr4.2:39818595-39818614 None:intergenic 25.0%
! ATAGTTTATATCTTTGTGCA+TGG - chr4.2:39817410-39817429 MS.gene003934:CDS 25.0%
! ATCAACAACAAATGAATTCA+GGG - chr4.2:39817904-39817923 MS.gene003934:CDS 25.0%
! CCATGTAAAACAAAGAAAAA+GGG + chr4.2:39818569-39818588 None:intergenic 25.0%
! GCAATAAATAGTAAACCTAT+TGG + chr4.2:39819102-39819121 None:intergenic 25.0%
! TCAAACATTAACATTGATAC+TGG - chr4.2:39818431-39818450 MS.gene003934:intron 25.0%
! TCAACTACTAAATCAGTTAT+AGG + chr4.2:39818131-39818150 None:intergenic 25.0%
! TCATGATAAGAAAAAGAGTA+TGG + chr4.2:39818464-39818483 None:intergenic 25.0%
! TGAAATGTACTATCAGAATT+TGG - chr4.2:39819524-39819543 MS.gene003934:intron 25.0%
! TTATAAATTCTGCTACACTT+CGG + chr4.2:39819663-39819682 None:intergenic 25.0%
!! AAATAAATTGATTTCCTTGG+TGG - chr4.2:39817712-39817731 MS.gene003934:intron 25.0%
!! AATAAATTGATTTCCTTGGT+GGG - chr4.2:39817713-39817732 MS.gene003934:intron 25.0%
!! GTGAAATAAATTGATTTCCT+TGG - chr4.2:39817709-39817728 MS.gene003934:intron 25.0%
!!! AATGGAAATAGGTATTGAAA+AGG + chr4.2:39818528-39818547 None:intergenic 25.0%
!!! ACTATTGTCTTTGTTGTTTT+TGG - chr4.2:39817522-39817541 MS.gene003934:intron 25.0%
!!! ATGGAAATAGGTATTGAAAA+GGG + chr4.2:39818527-39818546 None:intergenic 25.0%
!!! GTGACTTTTTTAAATAACTC+CGG - chr4.2:39818637-39818656 MS.gene003934:intron 25.0%
AAAAAAGAGGCAATGGAAAT+AGG + chr4.2:39818539-39818558 None:intergenic 30.0%
AAATGAATTCAGGGTTAACA+CGG - chr4.2:39817913-39817932 MS.gene003934:CDS 30.0%
AGAGGACTTGATTTAAATCT+AGG - chr4.2:39818985-39819004 MS.gene003934:CDS 30.0%
CATCAACAACAAATGAATTC+AGG - chr4.2:39817903-39817922 MS.gene003934:CDS 30.0%
GAAGAAGAAGAAGAAAAGAA+TGG - chr4.2:39817830-39817849 MS.gene003934:intron 30.0%
GAATTATGGTAAAAACTCTG+AGG + chr4.2:39818329-39818348 None:intergenic 30.0%
GTTCCTTGCTAAAATTATGA+TGG + chr4.2:39818811-39818830 None:intergenic 30.0%
TCAAACACTTATTCATGCTT+TGG - chr4.2:39820044-39820063 MS.gene003934:CDS 30.0%
TGATGATCTAGAAACATTGT+TGG + chr4.2:39817876-39817895 None:intergenic 30.0%
TTTAAATCCAGCTGAAACTA+AGG - chr4.2:39819497-39819516 MS.gene003934:intron 30.0%
! AAACAGAAAGAGTTTTTGCA+AGG - chr4.2:39818021-39818040 MS.gene003934:CDS 30.0%
! AGCAAATTTTTCTTCTTCTG+CGG - chr4.2:39819242-39819261 MS.gene003934:CDS 30.0%
! AGGTTTATGAATAGTCTTAG+TGG - chr4.2:39818041-39818060 MS.gene003934:CDS 30.0%
! AGTTTTTATCAAAATCACGG+TGG - chr4.2:39818707-39818726 MS.gene003934:intron 30.0%
! CAGAGTTTTTACCATAATTC+AGG - chr4.2:39818329-39818348 MS.gene003934:intron 30.0%
! CATCATCTCTGTATCTTTTT+AGG + chr4.2:39819452-39819471 None:intergenic 30.0%
! CTTGCTAAAATTATGATGGA+TGG + chr4.2:39818807-39818826 None:intergenic 30.0%
! TTGACAACACTGATTACATT+TGG - chr4.2:39819710-39819729 MS.gene003934:intron 30.0%
! TTTTAAATAACTCCGGAATC+CGG - chr4.2:39818644-39818663 MS.gene003934:intron 30.0%
!! CATCCATCATAATTTTAGCA+AGG - chr4.2:39818805-39818824 MS.gene003934:intron 30.0%
!! TTATTCATGCTTTGGAATGA+AGG - chr4.2:39820052-39820071 MS.gene003934:CDS 30.0%
!!! ACACTTTTTTGTTGTTGTGT+TGG + chr4.2:39817325-39817344 None:intergenic 30.0%
!!! ACTTTTTTGTTGTTGTGTTG+GGG + chr4.2:39817323-39817342 None:intergenic 30.0%
!!! CACTTTTTTGTTGTTGTGTT+GGG + chr4.2:39817324-39817343 None:intergenic 30.0%
!!! CCCTTTTTCTTTGTTTTACA+TGG - chr4.2:39818566-39818585 MS.gene003934:intron 30.0%
AAAGGGAATCACGAAAAAAG+AGG + chr4.2:39818552-39818571 None:intergenic 35.0%
AAGAAACCAAGTTCGATTTC+TGG + chr4.2:39817498-39817517 None:intergenic 35.0%
AATCACGAAAAAAGAGGCAA+TGG + chr4.2:39818546-39818565 None:intergenic 35.0%
ATAACAAAGAAAACCCACCA+AGG + chr4.2:39817729-39817748 None:intergenic 35.0%
ATCATCGTGTCAATCTAATC+CGG + chr4.2:39818666-39818685 None:intergenic 35.0%
ATGAGAGGAATAGGAACTAT+GGG - chr4.2:39819195-39819214 MS.gene003934:CDS 35.0%
ATGTTTGAAAACAATCCAGC+AGG + chr4.2:39818419-39818438 None:intergenic 35.0%
ATTGATCAGAATGAAACAGG+AGG - chr4.2:39819768-39819787 MS.gene003934:intron 35.0%
CAAAACAATCTTGAACCTGA+AGG - chr4.2:39819333-39819352 MS.gene003934:CDS 35.0%
CTATAAGAGACACTAGTAAC+AGG - chr4.2:39817444-39817463 MS.gene003934:CDS 35.0%
GAACTGCAAAAACTTCTCAA+TGG + chr4.2:39819859-39819878 None:intergenic 35.0%
GATGAGTATGCCAAATACTA+CGG - chr4.2:39819821-39819840 MS.gene003934:intron 35.0%
GCAAAAACTCTTTCTGTTTC+AGG + chr4.2:39818020-39818039 None:intergenic 35.0%
GCTACTTGACCTAAAAAAAC+GGG - chr4.2:39819037-39819056 MS.gene003934:CDS 35.0%
GCTGGAAAATGAGAACATAA+TGG - chr4.2:39818900-39818919 MS.gene003934:CDS 35.0%
GGCTACTTGACCTAAAAAAA+CGG - chr4.2:39819036-39819055 MS.gene003934:CDS 35.0%
TATAATGATGCTAGCTAGCT+TGG - chr4.2:39819154-39819173 MS.gene003934:CDS 35.0%
TATGAGAGGAATAGGAACTA+TGG - chr4.2:39819194-39819213 MS.gene003934:CDS 35.0%
TCAACCTCTTCCTTAACTAT+AGG + chr4.2:39818197-39818216 None:intergenic 35.0%
TCACTCAGCATCAAAACAAA+CGG + chr4.2:39817576-39817595 None:intergenic 35.0%
TTACCGAATCAAATGAAGAC+CGG - chr4.2:39818362-39818381 MS.gene003934:intron 35.0%
! ACATGAGCTCTTTTGTGTTA+TGG + chr4.2:39817614-39817633 None:intergenic 35.0%
! AGATTTTGCTGAATTCGTTG+TGG - chr4.2:39818737-39818756 MS.gene003934:intron 35.0%
! ATGAATAGTCTTAGTGGAAG+TGG - chr4.2:39818047-39818066 MS.gene003934:CDS 35.0%
! ATTTTTCTTCTTCTGCGGTA+CGG - chr4.2:39819247-39819266 MS.gene003934:CDS 35.0%
! CATTTTCCAGCTAATTGGAT+CGG + chr4.2:39818891-39818910 None:intergenic 35.0%
! GTTCTCATTTTCCAGCTAAT+TGG + chr4.2:39818896-39818915 None:intergenic 35.0%
! TCATGAAAGTACAACTCAGT+TGG - chr4.2:39818939-39818958 MS.gene003934:CDS 35.0%
! TGCATTGATCAGAATGAAAC+AGG - chr4.2:39819765-39819784 MS.gene003934:intron 35.0%
!! ACAAAAAAGTGTTGCACTAG+AGG - chr4.2:39817333-39817352 MS.gene003934:intron 35.0%
!! GTCTTCATTTGATTCGGTAA+AGG + chr4.2:39818362-39818381 None:intergenic 35.0%
!! TCAGGTTCAAGATTGTTTTG+TGG + chr4.2:39819333-39819352 None:intergenic 35.0%
!! TTCATTTGATTCGGTAAAGG+TGG + chr4.2:39818359-39818378 None:intergenic 35.0%
!!! CTTTTTTGTTGTTGTGTTGG+GGG + chr4.2:39817322-39817341 None:intergenic 35.0%
!!! TCACGAAATCCCGTTTTTTT+AGG + chr4.2:39819049-39819068 None:intergenic 35.0%
!!! TTTGTTTTGATGCTGAGTGA+TGG - chr4.2:39817576-39817595 MS.gene003934:intron 35.0%
!!! TTTTTTGTTGTTGTGTTGGG+GGG + chr4.2:39817321-39817340 None:intergenic 35.0%
AAGGTGGTCTTCCTGAATTA+TGG + chr4.2:39818343-39818362 None:intergenic 40.0%
ATACTCATCTGATGAGCCAA+AGG + chr4.2:39819810-39819829 None:intergenic 40.0%
CTTTCTGTTTCAGGACTTGA+AGG + chr4.2:39818011-39818030 None:intergenic 40.0%
GATGTAAAACCATTTCCTCC+AGG - chr4.2:39819474-39819493 MS.gene003934:CDS 40.0%
GCAACAGCTTCCTATAGTTA+AGG - chr4.2:39818184-39818203 MS.gene003934:intron 40.0%
GCTTCCTATAGTTAAGGAAG+AGG - chr4.2:39818190-39818209 MS.gene003934:intron 40.0%
GGATTTAAACCTGGAGGAAA+TGG + chr4.2:39819486-39819505 None:intergenic 40.0%
GTTACACCAGAAATCGAACT+TGG - chr4.2:39817489-39817508 MS.gene003934:intron 40.0%
GTTTCAGCTGGATTTAAACC+TGG + chr4.2:39819495-39819514 None:intergenic 40.0%
TCAACTACCTTAGTTTCAGC+TGG + chr4.2:39819507-39819526 None:intergenic 40.0%
TCTAATAAGACTAGACCGAC+CGG + chr4.2:39818384-39818403 None:intergenic 40.0%
TGTCAATCTAATCCGGATTC+CGG + chr4.2:39818659-39818678 None:intergenic 40.0%
TTTCCCCTTAGGTACTACAT+GGG - chr4.2:39819392-39819411 MS.gene003934:CDS 40.0%
TTTGCTGCTATGAGAGGAAT+AGG - chr4.2:39819186-39819205 MS.gene003934:CDS 40.0%
! AATAGTCTTAGTGGAAGTGG+TGG - chr4.2:39818050-39818069 MS.gene003934:CDS 40.0%
! AGTAGAGCATTTGACAACAC+TGG + chr4.2:39819559-39819578 None:intergenic 40.0%
! GAAAGTACAACTCAGTTGGT+AGG - chr4.2:39818943-39818962 MS.gene003934:CDS 40.0%
! TTTGTGCAGTGTTGTTAGTG+CGG - chr4.2:39819627-39819646 MS.gene003934:intron 40.0%
!! CGGTCTAGTCTTATTAGACA+TGG - chr4.2:39818386-39818405 MS.gene003934:intron 40.0%
!! TTTTAGGTCAAGTAGCCTAG+TGG + chr4.2:39819033-39819052 None:intergenic 40.0%
!!! TTTTTGTTGTTGTGTTGGGG+GGG + chr4.2:39817320-39817339 None:intergenic 40.0%
AATTGGATCGGCAGTCAGTT+CGG + chr4.2:39818879-39818898 None:intergenic 45.0%
ATCCTCCCATGTAGTACCTA+AGG + chr4.2:39819400-39819419 None:intergenic 45.0%
CGAATCAAATGAAGACCGGT+CGG - chr4.2:39818366-39818385 MS.gene003934:intron 45.0%
CGACCGGTCTTCATTTGATT+CGG + chr4.2:39818368-39818387 None:intergenic 45.0%
CTTCTCAATGGCTGCCAATT+CGG + chr4.2:39819847-39819866 None:intergenic 45.0%
GCAGGGACATTGATATACAG+AGG - chr4.2:39818967-39818986 MS.gene003934:CDS 45.0%
GTTTCCCCTTAGGTACTACA+TGG - chr4.2:39819391-39819410 MS.gene003934:CDS 45.0%
TCACCGTTAGGAGCTGAAAT+CGG - chr4.2:39819294-39819313 MS.gene003934:CDS 45.0%
TCAGCTGGATTTAAACCTGG+AGG + chr4.2:39819492-39819511 None:intergenic 45.0%
TCCTCCCATGTAGTACCTAA+GGG + chr4.2:39819399-39819418 None:intergenic 45.0%
TGCAAAATCCGTGCTAAACG+AGG - chr4.2:39819894-39819913 MS.gene003934:intron 45.0%
! ATGATGCTAGCTAGCTTGGT+TGG - chr4.2:39819158-39819177 MS.gene003934:CDS 45.0%
! CGTTTAGCACGGATTTTGCA+CGG + chr4.2:39819894-39819913 None:intergenic 45.0%
! CTGAAGGTTTTGCTGAAACC+CGG - chr4.2:39819349-39819368 MS.gene003934:CDS 45.0%
! GATGCTGAGTGATGGAAACT+TGG - chr4.2:39817584-39817603 MS.gene003934:intron 45.0%
! TGAAGGTTTTGCTGAAACCC+GGG - chr4.2:39819350-39819369 MS.gene003934:CDS 45.0%
!!! TTTTGTTGTTGTGTTGGGGG+GGG + chr4.2:39817319-39817338 None:intergenic 45.0%
!! AATTTATAATAGAATTAAAA+TGG + chr4.2:39819958-39819977 None:intergenic 5.0%
ACCCTTTCTGCGATGCTTCT+TGG + chr4.2:39819933-39819952 None:intergenic 50.0%
CACCGTTAGGAGCTGAAATC+GGG - chr4.2:39819295-39819314 MS.gene003934:CDS 50.0%
CCAAACTATGCCTCACCGTT+AGG - chr4.2:39819282-39819301 MS.gene003934:CDS 50.0%
CCAAAGGAGCAGAAGTAGTC+TGG + chr4.2:39819794-39819813 None:intergenic 50.0%
CCAGACTACTTCTGCTCCTT+TGG - chr4.2:39819791-39819810 MS.gene003934:intron 50.0%
CCCTTTCTGCGATGCTTCTT+GGG + chr4.2:39819932-39819951 None:intergenic 50.0%
CCGGGATAAAATCGTTACCC+CGG + chr4.2:39819371-39819390 None:intergenic 50.0%
CCTAACGGTGAGGCATAGTT+TGG + chr4.2:39819285-39819304 None:intergenic 50.0%
CCTCCCATGTAGTACCTAAG+GGG + chr4.2:39819398-39819417 None:intergenic 50.0%
CGGGATAAAATCGTTACCCC+GGG + chr4.2:39819370-39819389 None:intergenic 50.0%
CGGGTTTCAGCAAAACCTTC+AGG + chr4.2:39819351-39819370 None:intergenic 50.0%
CTCCCGATTTCAGCTCCTAA+CGG + chr4.2:39819300-39819319 None:intergenic 50.0%
CTTAGTGGAAGTGGTGGTAG+TGG - chr4.2:39818056-39818075 MS.gene003934:CDS 50.0%
GATTTCAGCTCCTAACGGTG+AGG + chr4.2:39819295-39819314 None:intergenic 50.0%
GCCAAATACTACGGCCGAAT+TGG - chr4.2:39819830-39819849 MS.gene003934:intron 50.0%
GCCAATTCGGCCGTAGTATT+TGG + chr4.2:39819834-39819853 None:intergenic 50.0%
GTAGCGTGTAGTGAAGAGAG+TGG + chr4.2:39817284-39817303 None:intergenic 50.0%
GTAGTACCTAAGGGGAAACC+CGG + chr4.2:39819390-39819409 None:intergenic 50.0%
TAGTACCTAAGGGGAAACCC+GGG + chr4.2:39819389-39819408 None:intergenic 50.0%
TGACTGCCGATCCAATTAGC+TGG - chr4.2:39818882-39818901 MS.gene003934:CDS 50.0%
TTTATCCCGGGTTTCCCCTT+AGG - chr4.2:39819381-39819400 MS.gene003934:CDS 50.0%
! CCGGGGTAACGATTTTATCC+CGG - chr4.2:39819368-39819387 MS.gene003934:CDS 50.0%
! CGGGGTAACGATTTTATCCC+GGG - chr4.2:39819369-39819388 MS.gene003934:CDS 50.0%
! GAAGGTTTTGCTGAAACCCG+GGG - chr4.2:39819351-39819370 MS.gene003934:CDS 50.0%
! GCAGGTTTTGCTGCTATGAG+AGG - chr4.2:39819180-39819199 MS.gene003934:CDS 50.0%
!! ACAACTCAGTTGGTAGGTGC+AGG - chr4.2:39818949-39818968 MS.gene003934:CDS 50.0%
!! ACCCAAGAAGCATCGCAGAA+AGG - chr4.2:39819928-39819947 MS.gene003934:intron 50.0%
!! CAACTCAGTTGGTAGGTGCA+GGG - chr4.2:39818950-39818969 MS.gene003934:CDS 50.0%
!! CCCAAGAAGCATCGCAGAAA+GGG - chr4.2:39819929-39819948 MS.gene003934:intron 50.0%
AGACATGGCAGTTCACCTGC+TGG - chr4.2:39818401-39818420 MS.gene003934:intron 55.0%
AGGTCAAGTAGCCTAGTGGC+AGG + chr4.2:39819029-39819048 None:intergenic 55.0%
CCCCTTAGGTACTACATGGG+AGG - chr4.2:39819395-39819414 MS.gene003934:CDS 55.0%
GCGTGTAGTGAAGAGAGTGG+TGG + chr4.2:39817281-39817300 None:intergenic 55.0%
TGCGATGCTTCTTGGGTGAG+TGG + chr4.2:39819925-39819944 None:intergenic 55.0%
TGGCACAGCCTCGTTTAGCA+CGG + chr4.2:39819905-39819924 None:intergenic 55.0%
! TGCTAGCTAGCTTGGTTGGC+AGG - chr4.2:39819162-39819181 MS.gene003934:CDS 55.0%
!! AGTGGAAGTGGTGGTAGTGG+TGG - chr4.2:39818059-39818078 MS.gene003934:CDS 55.0%
AAGTAGCCTAGTGGCAGGCG+TGG + chr4.2:39819024-39819043 None:intergenic 60.0%
ACTTCTCCACGCCTGCCACT+AGG - chr4.2:39819015-39819034 MS.gene003934:CDS 60.0%
TAGTGGCAGGCGTGGAGAAG+TGG + chr4.2:39819016-39819035 None:intergenic 60.0%
TTGTTGTGTTGGGGGGGGTG+TGG + chr4.2:39817314-39817333 None:intergenic 60.0%
!! AGTGGTGGTAGTGGTGGTGG+TGG - chr4.2:39818065-39818084 MS.gene003934:CDS 60.0%
!! GGAAGTGGTGGTAGTGGTGG+TGG - chr4.2:39818062-39818081 MS.gene003934:CDS 60.0%
!! GGTAGTGGTGGTGGTGGTGG+TGG - chr4.2:39818071-39818090 MS.gene003934:CDS 65.0%
!! GGTGGTAGTGGTGGTGGTGG+TGG - chr4.2:39818068-39818087 MS.gene003934:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 39817253 39820071 39817253 ID=MS.gene003934
chr4.2 mRNA 39817253 39820071 39817253 ID=MS.gene003934.t1;Parent=MS.gene003934
chr4.2 exon 39819970 39820071 39819970 ID=MS.gene003934.t1.exon1;Parent=MS.gene003934.t1
chr4.2 CDS 39819970 39820071 39819970 ID=cds.MS.gene003934.t1;Parent=MS.gene003934.t1
chr4.2 exon 39818872 39819505 39818872 ID=MS.gene003934.t1.exon2;Parent=MS.gene003934.t1
chr4.2 CDS 39818872 39819505 39818872 ID=cds.MS.gene003934.t1;Parent=MS.gene003934.t1
chr4.2 exon 39817848 39818140 39817848 ID=MS.gene003934.t1.exon3;Parent=MS.gene003934.t1
chr4.2 CDS 39817848 39818140 39817848 ID=cds.MS.gene003934.t1;Parent=MS.gene003934.t1
chr4.2 exon 39817374 39817468 39817374 ID=MS.gene003934.t1.exon4;Parent=MS.gene003934.t1
chr4.2 CDS 39817374 39817468 39817374 ID=cds.MS.gene003934.t1;Parent=MS.gene003934.t1
chr4.2 exon 39817253 39817295 39817253 ID=MS.gene003934.t1.exon5;Parent=MS.gene003934.t1
chr4.2 CDS 39817253 39817295 39817253 ID=cds.MS.gene003934.t1;Parent=MS.gene003934.t1
Gene Sequence

>MS.gene003934

AGGTTTCTTCACTTTCATTTTTACACCACCACTCTCTTCACTACACGCTACATTAAATCCACACCCCCCCCAACACAACAACAAAAAAGTGTTGCACTAGAGGTATTTTTTTTTTTGGTTTTATGTTACTGTTAAGTTCATAGTATTCATATTTTTCATAGTTTATATCTTTGTGCATGGTTTTTGTTTAACTATAAGAGACACTAGTAACAGGTAAGTACGTCTGATAAAAAATTGTTACACCAGAAATCGAACTTGGTTTCTTCCAAACTATTGTCTTTGTTGTTTTTGGTCATGTCTTGTCATAGCTAAACTGTTTGCCGTTTGTTTTGATGCTGAGTGATGGAAACTTGGAAAACCATAACACAAAAGAGCTCATGTTTTTCTTGTCTTGTTTTGTTTTTTGTTTTACTTTTTACACCCTCTTTCATCTTTTTCAGTTTTTGAAGAATTTTAGTGAAATAAATTGATTTCCTTGGTGGGTTTTCTTTGTTATTGATTTTTATTAGATCTTAGAAATTTTTATGTTGAAAATTTGAGTAAAGATCGAATTTTTATTTTTACAGGAATTGTTGAAGAAGAAGAAGAAGAAAAGAATGGAGTCAGATCTTCAGCAGCATCCAACAATGTTTCTAGATCATCAAAATCATCATCAACAACAAATGAATTCAGGGTTAACACGGTTTAAATCTGCTCCAAGTTCATATTTTTCAAATATCATTGATAAAGAGTTTTATGAGCATCTTTTCAATAAACCTTCAAGTCCTGAAACAGAAAGAGTTTTTGCAAGGTTTATGAATAGTCTTAGTGGAAGTGGTGGTAGTGGTGGTGGTGGTGGTGGTGCTGAAAGTGTTTCTGTTACAGCTTCTATAGCTCCTATAACTGATTTAGTAGTTGATTCATTGACTCAGAATCTATCAACTGTTCAACAGCAACAGCTTCCTATAGTTAAGGAAGAGGTTGATCAACAATCTCAAACTATGCAAACAATGAATAACAATGAATCTGTGGAACTTCAACAACTTCAAAGACAACAAAGTAATAATTTGAGTAATTATGGTTCTTCTGCTGCTCCTCAGAGTTTTTACCATAATTCAGGAAGACCACCTTTACCGAATCAAATGAAGACCGGTCGGTCTAGTCTTATTAGACATGGCAGTTCACCTGCTGGATTGTTTTCAAACATTAACATTGATACTGGTATGTGTCCATACTCTTTTTCTTATCATGAAAAAAATAAAACATCACTTTGTTTTGTGTTTTTTCTTCTTCCCTTTTCAATACCTATTTCCATTGCCTCTTTTTTCGTGATTCCCTTTTTCTTTGTTTTACATGGTAGCCTAAGTAGCTTTACTTTGTTATTAATAGAGACTTTTTGCTATTAGTGACTTTTTTAAATAACTCCGGAATCCGGATTAGATTGACACGATGATTGTATTGAAGCTATAAAGTGTAGTTTTTATCAAAATCACGGTGGATCAGTGAGATTTTGCTGAATTCGTTGTGGATCCAAATATGCACAAAATGTAAATTTGACTGAGTCATGATATGCCATCCATCATAATTTTAGCAAGGAACTACACTTTAGAGCTTTAATAAAATCACAGTGACGTCATGATTTCGCCGAACTGACTGCCGATCCAATTAGCTGGAAAATGAGAACATAATGGTTGTCTTAATAGAATTTCATGAAAGTACAACTCAGTTGGTAGGTGCAGGGACATTGATATACAGAGGACTTGATTTAAATCTAGGATTCTCCACTTCTCCACGCCTGCCACTAGGCTACTTGACCTAAAAAAACGGGATTTCGTGAAAGTGTGATAGAAAAAAGTTAATTAGAAATCCAATAGGTTTACTATTTATTGCAGTTTTCATTTTCTTGATCATAAATAGGTTGTTATAATGATGCTAGCTAGCTTGGTTGGCAGGTTTTGCTGCTATGAGAGGAATAGGAACTATGGGAGCTGCTAATAGCGCTAGCAAAGAAGCAAATTTTTCTTCTTCTGCGGTACGGTTAAAGAATGCTCCAAACTATGCCTCACCGTTAGGAGCTGAAATCGGGAGTAATAGCATTCCACAAAACAATCTTGAACCTGAAGGTTTTGCTGAAACCCGGGGTAACGATTTTATCCCGGGTTTCCCCTTAGGTACTACATGGGAGGATACTGCGATGATATCTGATAATATTACTGGCCTAAAAAGATACAGAGATGATGATGATGTAAAACCATTTCCTCCAGGTTTAAATCCAGCTGAAACTAAGGTAGTTGAAATGTACTATCAGAATTTGGTTATTAACACCAGTGTTGTCAAATGCTCTACTATAACGTAGCAGAAATTGAATTTATAGCTATGTTCTGCAATGCGCTATTTTGTGCAGTGTTGTTAGTGCGGCACAATAGCACCGAAGTGTAGCAGAATTTATAAAAAACACTATCTTTCTGTGATGTGCAATTGACAACACTGATTACATTTGGTGTATAATTTATCACTATTGACATAATTTTGCTGCATTGATCAGAATGAAACAGGAGGCCAGACTACTTCTGCTCCTTTGGCTCATCAGATGAGTATGCCAAATACTACGGCCGAATTGGCAGCCATTGAGAAGTTTTTGCAGTTCTCAGATTCTGTTCCGTGCAAAATCCGTGCTAAACGAGGCTGTGCCACTCACCCAAGAAGCATCGCAGAAAGGGTATCCATTTTAATTCTATTATAAATTATTCTGTTTTTTTTTCTTTCACTTGTACTTAATTTCTTGATTCTATAATAGAATATCTGATTTTTCTCAAACACTTATTCATGCTTTGGAATGA
Protein sequence

>MS.gene003934.t1

RFLHFHFYTTTLFTTRYIKSTPPPTQQQKSVALEELLKKKKKKRMESDLQQHPTMFLDHQNHHQQQMNSGLTRFKSAPSSYFSNIIDKEFYEHLFNKPSSPETERVFARFMNSLSGSGGSGGGGGGAESVSVTASIAPITDLVVDSLTQNLSTVQQQQLPIVKEEVDQQSQTMQTMNNNESVELQQLQRQQSNNLSNYGSSAAPQSFYHNSGRPPLPNQMKTGRSSLIRHGSSPAGLFSNINIDTGFAAMRGIGTMGAANSASKEANFSSSAVRLKNAPNYASPLGAEIGSNSIPQNNLEPEGFAETRGNDFIPGFPLGTTWEDTAMISDNITGLKRYRDDDDPLRSFCSSQILFRAKSVLNEAVPLTQEASQKGISDFSQTLIHALE