AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004851

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004851.t1 MTR_1g076130 99.699 332 1 0 1 332 1 332 0.0 681
MS.gene004851.t1 MTR_1g043040 93.675 332 21 0 1 332 1 332 0.0 623
MS.gene004851.t1 MTR_8g005980 84.498 329 51 0 3 331 85 413 0.0 559
MS.gene004851.t1 MTR_1g090730 39.818 329 191 4 3 328 92 416 5.91e-74 234
MS.gene004851.t1 MTR_1g090730 41.852 270 153 1 3 272 92 357 1.85e-62 204
MS.gene004851.t1 MTR_7g037650 73.913 46 12 0 15 60 24 69 7.01e-14 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004851.t1 AT1G04410 90.060 332 33 0 1 332 1 332 0.0 605
MS.gene004851.t1 AT5G43330 89.458 332 35 0 1 332 1 332 0.0 597
MS.gene004851.t1 AT5G56720 72.590 332 91 0 1 332 40 371 0.0 510
MS.gene004851.t1 AT5G58330 41.641 329 185 4 3 328 96 420 5.21e-79 248
MS.gene004851.t1 AT5G58330 41.641 329 185 4 3 328 97 421 5.83e-79 248
MS.gene004851.t1 AT5G58330 41.587 315 177 4 17 328 2 312 5.01e-76 237

Find 91 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGCATTCCCTCTTCTTAA+AGG 0.234412 1.4:+62189227 MS.gene004851:CDS
TGGAGGATCACGGGCTGGTC+AGG 0.252087 1.4:-62189143 None:intergenic
ATCACTGAACGGTGTTAAAA+TGG 0.283888 1.4:+62189193 MS.gene004851:CDS
TGACAAGATTGGACCATAAC+AGG 0.286946 1.4:+62190324 MS.gene004851:CDS
CACATTAGAGATTGGGTTCT+AGG 0.308508 1.4:+62190732 MS.gene004851:CDS
CATTTCTTGTTTGACAAGAT+TGG 0.308718 1.4:+62190313 MS.gene004851:CDS
ATCAAGGCATTGGTGTTTGC+CGG 0.326996 1.4:-62190254 None:intergenic
TGCAATATCATCAGGGAACC+TGG 0.363492 1.4:+62190846 MS.gene004851:intron
TGGAGTCAATATTGCTGTTA+TGG 0.370670 1.4:+62189419 MS.gene004851:CDS
CTCTTGTCCCTATGATTGCC+AGG 0.371373 1.4:+62189105 MS.gene004851:CDS
ACAGTTGCGTGGTTGACATC+AGG 0.383245 1.4:-62190437 None:intergenic
TGATTGCAGCACCCCGTTGT+TGG 0.384508 1.4:-62190656 None:intergenic
TTTGACCCAGAGCCCTGTTA+TGG 0.392891 1.4:-62190337 None:intergenic
ATCACTCCCCTGGCAATCAT+AGG 0.399565 1.4:-62189113 None:intergenic
GCTTTCTGAGGAGAAGGCTT+TGG 0.399799 1.4:+62191206 MS.gene004851:CDS
CTTGTGACCACATTAGAGAT+TGG 0.400822 1.4:+62190724 MS.gene004851:CDS
CATAGCTACCGTCCAACAAC+GGG 0.401578 1.4:+62190644 MS.gene004851:CDS
CACCAATGCCTTGATCTTGA+AGG 0.415103 1.4:+62190262 MS.gene004851:CDS
TTTAACACCGTTCAGTGATT+CGG 0.419237 1.4:-62189189 None:intergenic
GGCATTGGTGTTTGCCGGGT+TGG 0.424208 1.4:-62190249 None:intergenic
CCTGTTCGTGTTCTTGTCAC+TGG 0.433814 1.4:+62188387 MS.gene004851:CDS
CCAGTGACAAGAACACGAAC+AGG 0.438629 1.4:-62188387 None:intergenic
TTGTGACCACATTAGAGATT+GGG 0.444343 1.4:+62190725 MS.gene004851:CDS
CCGTTCAGTGATTCGGCTGC+TGG 0.450461 1.4:-62189182 None:intergenic
TCGCCATTGCGAGTTGTGAC+AGG 0.451805 1.4:-62190932 None:intergenic
TTTCTCAAGAGCAGAAGCTT+GGG 0.453532 1.4:-62189514 None:intergenic
GTTTCTCAAGAGCAGAAGCT+TGG 0.456679 1.4:-62189515 None:intergenic
ATTGGACCATAACAGGGCTC+TGG 0.463189 1.4:+62190331 MS.gene004851:CDS
TTCAGTGATTCGGCTGCTGG+TGG 0.473142 1.4:-62189179 None:intergenic
AATATTGCTGTTATGGTTGG+TGG 0.480289 1.4:+62189426 MS.gene004851:CDS
GTCAATATTGCTGTTATGGT+TGG 0.483183 1.4:+62189423 MS.gene004851:CDS
GGAGGATCACGGGCTGGTCA+GGG 0.487190 1.4:-62189142 None:intergenic
AACTCGTGGCTGATGATGCC+TGG 0.490237 1.4:+62190492 MS.gene004851:CDS
GTTCTTGTCACTGGTGCTGC+AGG 0.494198 1.4:+62188396 MS.gene004851:CDS
GGCGAATGGCAAATAGTTCA+AGG 0.495465 1.4:+62190950 MS.gene004851:CDS
TTCCTTCAAGATCAAGGCAT+TGG 0.495936 1.4:-62190264 None:intergenic
TGCTACAACTGATGTGGTTG+AGG 0.500221 1.4:+62189389 MS.gene004851:CDS
AGGGCCGAGCATCACTCCCC+TGG 0.504498 1.4:-62189123 None:intergenic
GGTTCTCGTTGTTGCCAACC+CGG 0.510041 1.4:+62190235 MS.gene004851:intron
AGAAGAGCTTTCTGAGGAGA+AGG 0.511032 1.4:+62191200 MS.gene004851:CDS
TCAAGGCATTGGTGTTTGCC+GGG 0.513479 1.4:-62190253 None:intergenic
AGCGAATTCCTTCAAGATCA+AGG 0.519920 1.4:-62190270 None:intergenic
TCAGGGAACCTGGGTATCGA+TGG 0.524300 1.4:+62190856 MS.gene004851:intron
GGTGGATTCCCTAGAAAAGA+AGG 0.528773 1.4:+62189444 MS.gene004851:CDS
CGGTGTTAAAATGGAGTTGG+TGG 0.529018 1.4:+62189202 MS.gene004851:CDS
TCAAGCATGTGGAGGATCAC+GGG 0.532929 1.4:-62189152 None:intergenic
CGCCATTGCGAGTTGTGACA+GGG 0.533982 1.4:-62190931 None:intergenic
ATTCCCTAGAAAAGAAGGTA+TGG 0.539427 1.4:+62189449 MS.gene004851:CDS
TCGATGGGAGTTTATTCTGA+TGG 0.539461 1.4:+62190872 MS.gene004851:CDS
GTCAAGCATGTGGAGGATCA+CGG 0.540541 1.4:-62189153 None:intergenic
CAGTTGCGTGGTTGACATCA+GGG 0.549966 1.4:-62190436 None:intergenic
AACTCAAACCTTTAAGAAGA+GGG 0.552886 1.4:-62189235 None:intergenic
TCACTCCCCTGGCAATCATA+GGG 0.553376 1.4:-62189112 None:intergenic
TTGGACCATAACAGGGCTCT+GGG 0.556375 1.4:+62190332 MS.gene004851:CDS
TGCAGCACCCCGTTGTTGGA+CGG 0.557691 1.4:-62190652 None:intergenic
GGATCTTACAATGTACCCGC+TGG 0.562224 1.4:+62190893 MS.gene004851:CDS
GACAGCAGAAGAGCTTTCTG+AGG 0.563983 1.4:+62191194 MS.gene004851:CDS
TCACAACTCGCAATGGCGAA+TGG 0.564475 1.4:+62190936 MS.gene004851:CDS
TTCCCTGTCACAACTCGCAA+TGG 0.566100 1.4:+62190929 MS.gene004851:CDS
GTGGTCACAAGCAGCGCTCG+CGG 0.568937 1.4:-62190712 None:intergenic
ATTGCCAGGGGAGTGATGCT+CGG 0.570606 1.4:+62189119 MS.gene004851:CDS
CATCATCAGCCACGAGTTCA+CGG 0.570980 1.4:-62190487 None:intergenic
AAACTCAAACCTTTAAGAAG+AGG 0.571371 1.4:-62189236 None:intergenic
TCTTGTCCCTATGATTGCCA+GGG 0.574263 1.4:+62189106 MS.gene004851:CDS
GCATGTGGAGGATCACGGGC+TGG 0.580601 1.4:-62189148 None:intergenic
TCAGTGATTCGGCTGCTGGT+GGG 0.585206 1.4:-62189178 None:intergenic
GATGTGGTTGAGGGATGCAC+TGG 0.588132 1.4:+62189399 MS.gene004851:CDS
CAGGGAACCTGGGTATCGAT+GGG 0.588520 1.4:+62190857 MS.gene004851:intron
AATGAATAGATTAGTCCAGC+GGG 0.591914 1.4:-62190908 None:intergenic
GCTACAACTGATGTGGTTGA+GGG 0.594490 1.4:+62189390 MS.gene004851:CDS
GACAAGATTGGACCATAACA+GGG 0.599626 1.4:+62190325 MS.gene004851:CDS
ATAAACTCCCATCGATACCC+AGG 0.604419 1.4:-62190864 None:intergenic
CTGGTGGGATGTCAAGCATG+TGG 0.608538 1.4:-62189163 None:intergenic
CAGGGTACTGACTTGATGAA+TGG 0.610269 1.4:-62190418 None:intergenic
CTAGAACCCAATCTCTAATG+TGG 0.612432 1.4:-62190731 None:intergenic
TCATAGCTACCGTCCAACAA+CGG 0.613333 1.4:+62190643 MS.gene004851:CDS
TGTTGTTGCTACAACTGATG+TGG 0.615089 1.4:+62189383 MS.gene004851:CDS
TCAGCCACGAGTTCACGGAC+AGG 0.623183 1.4:-62190482 None:intergenic
ACATGCTGCTGCAAACTGCA+AGG 0.624025 1.4:+62189536 MS.gene004851:CDS
GCAATATCATCAGGGAACCT+GGG 0.624982 1.4:+62190847 MS.gene004851:intron
GAACGGTGTTAAAATGGAGT+TGG 0.636523 1.4:+62189199 MS.gene004851:CDS
AAAGCCTGTCCGTGAACTCG+TGG 0.642217 1.4:+62190478 MS.gene004851:CDS
TTGGGTTCTAGGAACTCCTG+AGG 0.644314 1.4:+62190743 MS.gene004851:CDS
GGAAACAAGCTGTCTAAGAG+AGG 0.644922 1.4:-62191239 None:intergenic
CTTGTCCCTATGATTGCCAG+GGG 0.651791 1.4:+62189107 MS.gene004851:CDS
CCAGCAGCCGAATCACTGAA+CGG 0.657663 1.4:+62189182 MS.gene004851:CDS
CTAGAAAAGAAGGTATGGAG+AGG 0.663254 1.4:+62189454 MS.gene004851:CDS
GAATGAATAGATTAGTCCAG+CGG 0.674762 1.4:-62190909 None:intergenic
GTGGGATGTCAAGCATGTGG+AGG 0.682561 1.4:-62189160 None:intergenic
CAGTGACAAGAACACGAACA+GGG 0.694789 1.4:-62188386 None:intergenic
ATAGCTACCGTCCAACAACG+GGG 0.807747 1.4:+62190645 MS.gene004851:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAATTGCATAATTATAA+GGG - chr1.4:62188724-62188743 None:intergenic 15.0%
!! ATGTGAAAAATGTTATAATT+TGG + chr1.4:62190390-62190409 MS.gene004851:CDS 15.0%
!! CTTAATTTACAAATAAAAGA+CGG - chr1.4:62191052-62191071 None:intergenic 15.0%
!! TGTGAAAAATGTTATAATTT+GGG + chr1.4:62190391-62190410 MS.gene004851:CDS 15.0%
!! TTCAAATTCTTTATATTGAA+TGG + chr1.4:62190792-62190811 MS.gene004851:intron 15.0%
!! TTGATTTATATAGCAAATAA+AGG + chr1.4:62191078-62191097 MS.gene004851:intron 15.0%
!!! AAAAAATATTTTCCATTCTA+TGG + chr1.4:62189635-62189654 MS.gene004851:intron 15.0%
!!! ATTTTTTTTGACATAAAATG+CGG - chr1.4:62189622-62189641 None:intergenic 15.0%
!! ACAACTTAATTAAATTCGTA+TGG - chr1.4:62189924-62189943 None:intergenic 20.0%
!! ATTTCAACATAAAATCTTTG+AGG + chr1.4:62189024-62189043 MS.gene004851:intron 20.0%
!! ATTTCTATAAACTCTCAAAT+AGG - chr1.4:62188775-62188794 None:intergenic 20.0%
!! CAGAAAATTGCATAATTATA+AGG - chr1.4:62188725-62188744 None:intergenic 20.0%
!! GTGAAAAATGTTATAATTTG+GGG + chr1.4:62190392-62190411 MS.gene004851:CDS 20.0%
!! GTTTCCAAAAATATGTAAAA+AGG - chr1.4:62190159-62190178 None:intergenic 20.0%
!! TTGTGAAAATTATTTGTTTC+AGG + chr1.4:62189360-62189379 MS.gene004851:intron 20.0%
!! TTTAGAAATAGAACTATGTT+TGG + chr1.4:62188552-62188571 MS.gene004851:intron 20.0%
!! TTTCTATAAACTCTCAAATA+GGG - chr1.4:62188774-62188793 None:intergenic 20.0%
!!! AAAAATGTTTTGCCAATTTT+AGG + chr1.4:62189068-62189087 MS.gene004851:intron 20.0%
!!! AGATTTTGATAAATTATTGC+TGG + chr1.4:62190975-62190994 MS.gene004851:intron 20.0%
!!! CGTCTTTTATTTGTAAATTA+AGG + chr1.4:62191050-62191069 MS.gene004851:intron 20.0%
!!! CTTTTGTTTTGTTTTGTTTA+AGG + chr1.4:62188457-62188476 MS.gene004851:intron 20.0%
!!! GTAAACATTTTAACCATATT+TGG - chr1.4:62188990-62189009 None:intergenic 20.0%
!!! TACGAATTTAATTAAGTTGT+TGG + chr1.4:62189924-62189943 MS.gene004851:intron 20.0%
!!! TAGTCCTTTTTACATATTTT+TGG + chr1.4:62190152-62190171 MS.gene004851:intron 20.0%
! AAATGCTTTGCAAGATTAAA+AGG + chr1.4:62189335-62189354 MS.gene004851:intron 25.0%
! AACTAAAATAAATTCAGTGC+AGG - chr1.4:62190017-62190036 None:intergenic 25.0%
! ACAGAGTTTAAAGAAATGTA+AGG - chr1.4:62190204-62190223 None:intergenic 25.0%
! AGGTAACCATAATAAATTCT+GGG + chr1.4:62188416-62188435 MS.gene004851:intron 25.0%
! ATACTTCAGTAAATGCAAAA+TGG - chr1.4:62190587-62190606 None:intergenic 25.0%
! TATCCAATTTGTCCTAAAAT+TGG - chr1.4:62189083-62189102 None:intergenic 25.0%
! TGAAAGAGATACATGAAAAA+GGG - chr1.4:62189671-62189690 None:intergenic 25.0%
! TGAAGTTGTTTATCCAAATA+TGG + chr1.4:62188974-62188993 MS.gene004851:intron 25.0%
! TTTATTTCCATAATCTCTCT+AGG + chr1.4:62188826-62188845 MS.gene004851:intron 25.0%
!! AAACAAGAAATGTTTTTCTC+AGG - chr1.4:62190305-62190324 None:intergenic 25.0%
!! TAAATGTTACTTGAACTTCT+TGG + chr1.4:62189269-62189288 MS.gene004851:intron 25.0%
!! TATCAATTGATGAGTTTTCA+AGG + chr1.4:62191157-62191176 MS.gene004851:CDS 25.0%
!! TTTTCATTGCAATATCATCA+GGG + chr1.4:62190839-62190858 MS.gene004851:intron 25.0%
!!! ATCTGTCTAATTTTTTAACC+AGG + chr1.4:62191132-62191151 MS.gene004851:intron 25.0%
!!! ATGTGTATTTTGGTCATATA+TGG + chr1.4:62190071-62190090 MS.gene004851:intron 25.0%
!!! TGAGTTTTCAAGGAAAAAAT+TGG + chr1.4:62191167-62191186 MS.gene004851:CDS 25.0%
AAAGAGATACATGAAAAAGG+GGG - chr1.4:62189669-62189688 None:intergenic 30.0%
AAGTTATCCTAGAGAGATTA+TGG - chr1.4:62188836-62188855 None:intergenic 30.0%
ATATTGAATGGCTTCAACTA+AGG + chr1.4:62190804-62190823 MS.gene004851:intron 30.0%
CAATCAAGAAACATGATACA+AGG - chr1.4:62188526-62188545 None:intergenic 30.0%
CAGGTAACCATAATAAATTC+TGG + chr1.4:62188415-62188434 MS.gene004851:intron 30.0%
CTGAAAGAGATACATGAAAA+AGG - chr1.4:62189672-62189691 None:intergenic 30.0%
CTTGTATCATGTTTCTTGAT+TGG + chr1.4:62188524-62188543 MS.gene004851:intron 30.0%
GAAAGAGATACATGAAAAAG+GGG - chr1.4:62189670-62189689 None:intergenic 30.0%
GAGAAACCCAGAATTTATTA+TGG - chr1.4:62188425-62188444 None:intergenic 30.0%
TAGGGAAAATACTACAATTG+AGG - chr1.4:62188756-62188775 None:intergenic 30.0%
TCAAATAAGTCAATCCAAAC+AGG - chr1.4:62189696-62189715 None:intergenic 30.0%
! AAACTCAAACCTTTAAGAAG+AGG - chr1.4:62189239-62189258 None:intergenic 30.0%
! AACTCAAACCTTTAAGAAGA+GGG - chr1.4:62189238-62189257 None:intergenic 30.0%
! AGAAATGTTTTTCTCAGGAA+TGG - chr1.4:62190300-62190319 None:intergenic 30.0%
! CATTTCTTGTTTGACAAGAT+TGG + chr1.4:62190313-62190332 MS.gene004851:CDS 30.0%
! CTTTTCATTGCAATATCATC+AGG + chr1.4:62190838-62190857 MS.gene004851:intron 30.0%
! TGATGTAATTTTCATCGTCA+AGG + chr1.4:62190607-62190626 MS.gene004851:intron 30.0%
! TTAGTTTAGTGCTGAAAAAG+TGG + chr1.4:62190031-62190050 MS.gene004851:intron 30.0%
! TTTTCATCGTCAAGGTTAAA+TGG + chr1.4:62190615-62190634 MS.gene004851:intron 30.0%
!!! TTGCCAATTTTAGGACAAAT+TGG + chr1.4:62189077-62189096 MS.gene004851:intron 30.0%
AGAAACAGATTTCACCAGAA+TGG - chr1.4:62190113-62190132 None:intergenic 35.0%
ATTCAGTGCAGGTAATGTAT+TGG - chr1.4:62190006-62190025 None:intergenic 35.0%
ATTCCCTAGAAAAGAAGGTA+TGG + chr1.4:62189449-62189468 MS.gene004851:CDS 35.0%
CCAACTTCAAACCAGAAATA+TGG - chr1.4:62189299-62189318 None:intergenic 35.0%
CTTATTTCCATAAGCTCTAC+AGG + chr1.4:62189805-62189824 MS.gene004851:intron 35.0%
GCTTGAGCATTCAGAATTAA+AGG - chr1.4:62189566-62189585 None:intergenic 35.0%
TATTTGTCCATCAACATACC+AGG - chr1.4:62190513-62190532 None:intergenic 35.0%
TTGTGACCACATTAGAGATT+GGG + chr1.4:62190725-62190744 MS.gene004851:CDS 35.0%
TTTAACACCGTTCAGTGATT+CGG - chr1.4:62189192-62189211 None:intergenic 35.0%
! AACTCATCAATTGATAGTCC+TGG - chr1.4:62191153-62191172 None:intergenic 35.0%
! AATATTGCTGTTATGGTTGG+TGG + chr1.4:62189426-62189445 MS.gene004851:CDS 35.0%
! AATGAATAGATTAGTCCAGC+GGG - chr1.4:62190911-62190930 None:intergenic 35.0%
! ATCACTGAACGGTGTTAAAA+TGG + chr1.4:62189193-62189212 MS.gene004851:CDS 35.0%
! ATGTTTTTCTCAGGAATGGA+TGG - chr1.4:62190296-62190315 None:intergenic 35.0%
! GAATGAATAGATTAGTCCAG+CGG - chr1.4:62190912-62190931 None:intergenic 35.0%
! GTCAATATTGCTGTTATGGT+TGG + chr1.4:62189423-62189442 MS.gene004851:CDS 35.0%
! TCTCCATACCTTCTTTTCTA+GGG - chr1.4:62189455-62189474 None:intergenic 35.0%
! TGGAGTCAATATTGCTGTTA+TGG + chr1.4:62189419-62189438 MS.gene004851:CDS 35.0%
!! AAGCAATTTTAACAGTTGCG+TGG - chr1.4:62190451-62190470 None:intergenic 35.0%
!! CCATATTTCTGGTTTGAAGT+TGG + chr1.4:62189296-62189315 MS.gene004851:intron 35.0%
!! TGGTTGGTTCATGTGTATTT+TGG + chr1.4:62190061-62190080 MS.gene004851:intron 35.0%
AAAAAGTGGTTCCATCTGGT+TGG + chr1.4:62190045-62190064 MS.gene004851:intron 40.0%
AAGCTATCCTGTAGAGCTTA+TGG - chr1.4:62189815-62189834 None:intergenic 40.0%
AAGTCCTTGTGATTCCATTC+TGG + chr1.4:62190096-62190115 MS.gene004851:intron 40.0%
AGCGAATTCCTTCAAGATCA+AGG - chr1.4:62190273-62190292 None:intergenic 40.0%
ATACACATGAACCAACCAGA+TGG - chr1.4:62190059-62190078 None:intergenic 40.0%
CACATTAGAGATTGGGTTCT+AGG + chr1.4:62190732-62190751 MS.gene004851:CDS 40.0%
CTAGAACCCAATCTCTAATG+TGG - chr1.4:62190734-62190753 None:intergenic 40.0%
CTTGTGACCACATTAGAGAT+TGG + chr1.4:62190724-62190743 MS.gene004851:CDS 40.0%
GACAAGATTGGACCATAACA+GGG + chr1.4:62190325-62190344 MS.gene004851:CDS 40.0%
GTATCTCTTTCAGACCTGTT+TGG + chr1.4:62189679-62189698 MS.gene004851:intron 40.0%
TAAACTCTGTATCACCATGC+AGG + chr1.4:62190214-62190233 MS.gene004851:intron 40.0%
TCGATGGGAGTTTATTCTGA+TGG + chr1.4:62190872-62190891 MS.gene004851:CDS 40.0%
TGACAAGATTGGACCATAAC+AGG + chr1.4:62190324-62190343 MS.gene004851:CDS 40.0%
TGTTGTTGCTACAACTGATG+TGG + chr1.4:62189383-62189402 MS.gene004851:CDS 40.0%
TTCACCAGAATGGAATCACA+AGG - chr1.4:62190103-62190122 None:intergenic 40.0%
TTCCTTCAAGATCAAGGCAT+TGG - chr1.4:62190267-62190286 None:intergenic 40.0%
TTCTTGGACAGCCATATTTC+TGG + chr1.4:62189285-62189304 MS.gene004851:intron 40.0%
TTTCTCAAGAGCAGAAGCTT+GGG - chr1.4:62189517-62189536 None:intergenic 40.0%
! CTAGAAAAGAAGGTATGGAG+AGG + chr1.4:62189454-62189473 MS.gene004851:CDS 40.0%
! CTCTCCATACCTTCTTTTCT+AGG - chr1.4:62189456-62189475 None:intergenic 40.0%
! GAACGGTGTTAAAATGGAGT+TGG + chr1.4:62189199-62189218 MS.gene004851:CDS 40.0%
! GGTTTGAAGTTGGATTGTGT+TGG + chr1.4:62189306-62189325 MS.gene004851:intron 40.0%
AAGCACTTGTGAGACTGCTT+GGG + chr1.4:62189736-62189755 MS.gene004851:intron 45.0%
AGCAAGACATAACCAACCTC+AGG - chr1.4:62190762-62190781 None:intergenic 45.0%
ATAAACTCCCATCGATACCC+AGG - chr1.4:62190867-62190886 None:intergenic 45.0%
ATACATGAAAAAGGGGGAGG+AGG - chr1.4:62189663-62189682 None:intergenic 45.0%
CACCAATGCCTTGATCTTGA+AGG + chr1.4:62190262-62190281 MS.gene004851:CDS 45.0%
CAGTGACAAGAACACGAACA+GGG - chr1.4:62188389-62188408 None:intergenic 45.0%
GAGATACATGAAAAAGGGGG+AGG - chr1.4:62189666-62189685 None:intergenic 45.0%
GATGATGCCTGGTATGTTGA+TGG + chr1.4:62190503-62190522 MS.gene004851:intron 45.0%
GCAATATCATCAGGGAACCT+GGG + chr1.4:62190847-62190866 MS.gene004851:intron 45.0%
GCTACAACTGATGTGGTTGA+GGG + chr1.4:62189390-62189409 MS.gene004851:CDS 45.0%
GCTGAAAAAGTGGTTCCATC+TGG + chr1.4:62190041-62190060 MS.gene004851:intron 45.0%
GCTGCATTCCCTCTTCTTAA+AGG + chr1.4:62189227-62189246 MS.gene004851:CDS 45.0%
GGCGAATGGCAAATAGTTCA+AGG + chr1.4:62190950-62190969 MS.gene004851:CDS 45.0%
GTCTCACAAGTGCTTGCTTA+TGG - chr1.4:62189731-62189750 None:intergenic 45.0%
GTTTCTCAAGAGCAGAAGCT+TGG - chr1.4:62189518-62189537 None:intergenic 45.0%
TCATAGCTACCGTCCAACAA+CGG + chr1.4:62190643-62190662 MS.gene004851:CDS 45.0%
TCTTGTCCCTATGATTGCCA+GGG + chr1.4:62189106-62189125 MS.gene004851:CDS 45.0%
TGCAATATCATCAGGGAACC+TGG + chr1.4:62190846-62190865 MS.gene004851:intron 45.0%
TGCTACAACTGATGTGGTTG+AGG + chr1.4:62189389-62189408 MS.gene004851:CDS 45.0%
! AGAAGAGCTTTCTGAGGAGA+AGG + chr1.4:62191200-62191219 MS.gene004851:CDS 45.0%
! CGGTGTTAAAATGGAGTTGG+TGG + chr1.4:62189202-62189221 MS.gene004851:CDS 45.0%
! GGTGGATTCCCTAGAAAAGA+AGG + chr1.4:62189444-62189463 MS.gene004851:CDS 45.0%
!! ATCAAGGCATTGGTGTTTGC+CGG - chr1.4:62190257-62190276 None:intergenic 45.0%
!! CAGGGTACTGACTTGATGAA+TGG - chr1.4:62190421-62190440 None:intergenic 45.0%
ACAGTTGCGTGGTTGACATC+AGG - chr1.4:62190440-62190459 None:intergenic 50.0%
ACATGCTGCTGCAAACTGCA+AGG + chr1.4:62189536-62189555 MS.gene004851:CDS 50.0%
ATAGCTACCGTCCAACAACG+GGG + chr1.4:62190645-62190664 MS.gene004851:CDS 50.0%
ATCACTCCCCTGGCAATCAT+AGG - chr1.4:62189116-62189135 None:intergenic 50.0%
ATTGGACCATAACAGGGCTC+TGG + chr1.4:62190331-62190350 MS.gene004851:CDS 50.0%
CAAGCACTTGTGAGACTGCT+TGG + chr1.4:62189735-62189754 MS.gene004851:intron 50.0%
CAGTTGCGTGGTTGACATCA+GGG - chr1.4:62190439-62190458 None:intergenic 50.0%
CATAGCTACCGTCCAACAAC+GGG + chr1.4:62190644-62190663 MS.gene004851:CDS 50.0%
CATCATCAGCCACGAGTTCA+CGG - chr1.4:62190490-62190509 None:intergenic 50.0%
CCAGTGACAAGAACACGAAC+AGG - chr1.4:62188390-62188409 None:intergenic 50.0%
CCTGTTCGTGTTCTTGTCAC+TGG + chr1.4:62188387-62188406 MS.gene004851:CDS 50.0%
CTCTTGTCCCTATGATTGCC+AGG + chr1.4:62189105-62189124 MS.gene004851:CDS 50.0%
CTTGTCCCTATGATTGCCAG+GGG + chr1.4:62189107-62189126 MS.gene004851:CDS 50.0%
GACAGCAGAAGAGCTTTCTG+AGG + chr1.4:62191194-62191213 MS.gene004851:CDS 50.0%
GGATCTTACAATGTACCCGC+TGG + chr1.4:62190893-62190912 MS.gene004851:CDS 50.0%
GTCAAGCATGTGGAGGATCA+CGG - chr1.4:62189156-62189175 None:intergenic 50.0%
TCAAGCATGTGGAGGATCAC+GGG - chr1.4:62189155-62189174 None:intergenic 50.0%
TCACAACTCGCAATGGCGAA+TGG + chr1.4:62190936-62190955 MS.gene004851:CDS 50.0%
TCACTCCCCTGGCAATCATA+GGG - chr1.4:62189115-62189134 None:intergenic 50.0%
TTCCCTGTCACAACTCGCAA+TGG + chr1.4:62190929-62190948 MS.gene004851:CDS 50.0%
TTGGACCATAACAGGGCTCT+GGG + chr1.4:62190332-62190351 MS.gene004851:CDS 50.0%
TTTGACCCAGAGCCCTGTTA+TGG - chr1.4:62190340-62190359 None:intergenic 50.0%
! GCTTTCTGAGGAGAAGGCTT+TGG + chr1.4:62191206-62191225 MS.gene004851:CDS 50.0%
!! GTTCTAGGAACTCCTGAGGT+TGG + chr1.4:62190747-62190766 MS.gene004851:intron 50.0%
!! TCAAGGCATTGGTGTTTGCC+GGG - chr1.4:62190256-62190275 None:intergenic 50.0%
!! TTGGGTTCTAGGAACTCCTG+AGG + chr1.4:62190743-62190762 MS.gene004851:CDS 50.0%
AAAGCCTGTCCGTGAACTCG+TGG + chr1.4:62190478-62190497 MS.gene004851:CDS 55.0%
AGACTGCTTGGGAGAGCTCA+TGG + chr1.4:62189747-62189766 MS.gene004851:intron 55.0%
ATTGCCAGGGGAGTGATGCT+CGG + chr1.4:62189119-62189138 MS.gene004851:CDS 55.0%
CCAGCAGCCGAATCACTGAA+CGG + chr1.4:62189182-62189201 MS.gene004851:CDS 55.0%
CGCCATTGCGAGTTGTGACA+GGG - chr1.4:62190934-62190953 None:intergenic 55.0%
CTGGTGGGATGTCAAGCATG+TGG - chr1.4:62189166-62189185 None:intergenic 55.0%
GATGTGGTTGAGGGATGCAC+TGG + chr1.4:62189399-62189418 MS.gene004851:CDS 55.0%
GGCAACAACGAGAACCTGCA+TGG - chr1.4:62190231-62190250 None:intergenic 55.0%
GGGGAGGAGGAACCATAGAA+TGG - chr1.4:62189650-62189669 None:intergenic 55.0%
GGTTCTCGTTGTTGCCAACC+CGG + chr1.4:62190235-62190254 MS.gene004851:intron 55.0%
GTGGGATGTCAAGCATGTGG+AGG - chr1.4:62189163-62189182 None:intergenic 55.0%
TCGCCATTGCGAGTTGTGAC+AGG - chr1.4:62190935-62190954 None:intergenic 55.0%
TGATTGCAGCACCCCGTTGT+TGG - chr1.4:62190659-62190678 None:intergenic 55.0%
! AACTCGTGGCTGATGATGCC+TGG + chr1.4:62190492-62190511 MS.gene004851:CDS 55.0%
! TCAGTGATTCGGCTGCTGGT+GGG - chr1.4:62189181-62189200 None:intergenic 55.0%
! TTCAGTGATTCGGCTGCTGG+TGG - chr1.4:62189182-62189201 None:intergenic 55.0%
!! CAGGGAACCTGGGTATCGAT+GGG + chr1.4:62190857-62190876 MS.gene004851:intron 55.0%
!! GTTCTTGTCACTGGTGCTGC+AGG + chr1.4:62188396-62188415 MS.gene004851:CDS 55.0%
!! TCAGGGAACCTGGGTATCGA+TGG + chr1.4:62190856-62190875 MS.gene004851:intron 55.0%
TCAGCCACGAGTTCACGGAC+AGG - chr1.4:62190485-62190504 None:intergenic 60.0%
TGCAGCACCCCGTTGTTGGA+CGG - chr1.4:62190655-62190674 None:intergenic 60.0%
! CCGTTCAGTGATTCGGCTGC+TGG - chr1.4:62189185-62189204 None:intergenic 60.0%
!! GGCATTGGTGTTTGCCGGGT+TGG - chr1.4:62190252-62190271 None:intergenic 60.0%
GCATGTGGAGGATCACGGGC+TGG - chr1.4:62189151-62189170 None:intergenic 65.0%
GTGGTCACAAGCAGCGCTCG+CGG - chr1.4:62190715-62190734 None:intergenic 65.0%
!! GGAGGATCACGGGCTGGTCA+GGG - chr1.4:62189145-62189164 None:intergenic 65.0%
!! TGGAGGATCACGGGCTGGTC+AGG - chr1.4:62189146-62189165 None:intergenic 65.0%
AGGGCCGAGCATCACTCCCC+TGG - chr1.4:62189126-62189145 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 62188375 62191248 62188375 ID=MS.gene004851
chr1.4 mRNA 62188375 62191248 62188375 ID=MS.gene004851.t1;Parent=MS.gene004851
chr1.4 exon 62188375 62188417 62188375 ID=MS.gene004851.t1.exon1;Parent=MS.gene004851.t1
chr1.4 CDS 62188375 62188417 62188375 ID=cds.MS.gene004851.t1;Parent=MS.gene004851.t1
chr1.4 exon 62189090 62189248 62189090 ID=MS.gene004851.t1.exon2;Parent=MS.gene004851.t1
chr1.4 CDS 62189090 62189248 62189090 ID=cds.MS.gene004851.t1;Parent=MS.gene004851.t1
chr1.4 exon 62189382 62189557 62189382 ID=MS.gene004851.t1.exon3;Parent=MS.gene004851.t1
chr1.4 CDS 62189382 62189557 62189382 ID=cds.MS.gene004851.t1;Parent=MS.gene004851.t1
chr1.4 exon 62190236 62190513 62190236 ID=MS.gene004851.t1.exon4;Parent=MS.gene004851.t1
chr1.4 CDS 62190236 62190513 62190236 ID=cds.MS.gene004851.t1;Parent=MS.gene004851.t1
chr1.4 exon 62190629 62190764 62190629 ID=MS.gene004851.t1.exon5;Parent=MS.gene004851.t1
chr1.4 CDS 62190629 62190764 62190629 ID=cds.MS.gene004851.t1;Parent=MS.gene004851.t1
chr1.4 exon 62190860 62190971 62190860 ID=MS.gene004851.t1.exon6;Parent=MS.gene004851.t1
chr1.4 CDS 62190860 62190971 62190860 ID=cds.MS.gene004851.t1;Parent=MS.gene004851.t1
chr1.4 exon 62191154 62191248 62191154 ID=MS.gene004851.t1.exon7;Parent=MS.gene004851.t1
chr1.4 CDS 62191154 62191248 62191154 ID=cds.MS.gene004851.t1;Parent=MS.gene004851.t1
Gene Sequence

>MS.gene004851

ATGGCTAAAGACCCTGTTCGTGTTCTTGTCACTGGTGCTGCAGGTAACCATAATAAATTCTGGGTTTCTCTCAAAATTATGTCTTTTGTTTTGTTTTGTTTAAGGATATTTTATTATCATATTTTTTTTTTATCAGATCTGATCTGATCCTTGTATCATGTTTCTTGATTGGTTTTATTTAGAAATAGAACTATGTTTGGTTTATAATTGCATTAGCTCTTGATTTCTCATGATATGTGCTTACTCTATGATCAATTAAGAAAATAAAAAATTCTAGAAGTTGCAAGCAGTTAATATATTTTTTAAAAAATTTATATGCATTTACTTTCATTTGTTAAAAATATATCCCTTATAATTATGCAATTTTCTGATAGTATACCTCAATTGTAGTATTTTCCCTATTTGAGAGTTTATAGAAATAACATATGACATGTTTATAAGTTGTTTTCAGTTTATTTCCATAATCTCTCTAGGATAACTTGTGAAAAAAAGCTTGTAGTATATATGAAAACAATTTGTCTTTATTTTAACTTTTGATATAGAAATAGCTTATGCATAATCATTTATACATTTTAAGCATTTATGCTATAAGTGTTTAGTGAAGTTGTTTATCCAAATATGGTTAAAATGTTTACAAAAGCAATTTTTGATTTCAACATAAAATCTTTGAGGCATATTTTTTTTTTCGTACTAAAAAATGTTTTGCCAATTTTAGGACAAATTGGATATGCTCTTGTCCCTATGATTGCCAGGGGAGTGATGCTCGGCCCTGACCAGCCCGTGATCCTCCACATGCTTGACATCCCACCAGCAGCCGAATCACTGAACGGTGTTAAAATGGAGTTGGTGGATGCTGCATTCCCTCTTCTTAAAGGTTTGAGTTTTATTCTTTGCTAAATGTTACTTGAACTTCTTGGACAGCCATATTTCTGGTTTGAAGTTGGATTGTGTTGGTTGATTAAATGCTTTGCAAGATTAAAAGGTATTGTGAAAATTATTTGTTTCAGGTGTTGTTGCTACAACTGATGTGGTTGAGGGATGCACTGGAGTCAATATTGCTGTTATGGTTGGTGGATTCCCTAGAAAAGAAGGTATGGAGAGGAAAGATGTGATGACAAAAAATGTCTCTATTTACAAGTCCCAAGCTTCTGCTCTTGAGAAACATGCTGCTGCAAACTGCAAGGTGATCCTTTAATTCTGAATGCTCAAGCTTTTGTCTTAATTAATTTCAATTTCTAGTTCTGCCGCATTTTATGTCAAAAAAAATATTTTCCATTCTATGGTTCCTCCTCCCCCTTTTTCATGTATCTCTTTCAGACCTGTTTGGATTGACTTATTTGAACTTATATACTACCATAAGCAAGCACTTGTGAGACTGCTTGGGAGAGCTCATGGAAACAGCTCATGACATGTTCGTGAGATGTTTTCAGCTTATTTCCATAAGCTCTACAGGATAGCTTATGAAAACAGTTCATAGTTTATATGAAAACAATTTGACTTTATTTTATTGTTTTTTATAGAAATAGCTTACACATAAGCACTTATGCCATACGAATTTAATTAAGTTGTTGGCTGATCGATTGATTGAACGCTATTATTGTCATAGTTTTTGTGTGATAAGTGTAAAGCCAATACATTACCTGCACTGAATTTATTTTAGTTTAGTGCTGAAAAAGTGGTTCCATCTGGTTGGTTCATGTGTATTTTGGTCATATATGGCAAAGTCCTTGTGATTCCATTCTGGTGAAATCTGTTTCTTTTCTTCACAGAAAATGAGTAGTCCTTTTTACATATTTTTGGAAACATCAATTACATTTTATGTATTTCCTTACATTTCTTTAAACTCTGTATCACCATGCAGGTTCTCGTTGTTGCCAACCCGGCAAACACCAATGCCTTGATCTTGAAGGAATTCGCTCCATCCATTCCTGAGAAAAACATTTCTTGTTTGACAAGATTGGACCATAACAGGGCTCTGGGTCAAATTTCTGAAAGACTGAACGTTGAAGTTTCTAATGTGAAAAATGTTATAATTTGGGGCAACCATTCATCAAGTCAGTACCCTGATGTCAACCACGCAACTGTTAAAATTGCTTCTGCAGAAAAGCCTGTCCGTGAACTCGTGGCTGATGATGCCTGGTATGTTGATGGACAAATACAATGACTTGCAATTTAAACTTTTTTTTACGTATTTATTACATTTTTTTTTCCATTTTGCATTTACTGAAGTATTGATGTAATTTTCATCGTCAAGGTTAAATGGAGAATTCATAGCTACCGTCCAACAACGGGGTGCTGCAATCATTAAAGCTAGAAAGCTTTCAAGTGCACTATCTGCCGCGAGCGCTGCTTGTGACCACATTAGAGATTGGGTTCTAGGAACTCCTGAGGTTGGTTATGTCTTGCTCAATCTTTGATTCAAATTCTTTATATTGAATGGCTTCAACTAAGGTTTATGTTTATCTTTTCATTGCAATATCATCAGGGAACCTGGGTATCGATGGGAGTTTATTCTGATGGATCTTACAATGTACCCGCTGGACTAATCTATTCATTCCCTGTCACAACTCGCAATGGCGAATGGCAAATAGTTCAAGGTAAGATTTTGATAAATTATTGCTGGCTCTTGAATTCTTATTGTTTTAAGTGTTAATACTTCATACTTAACGTTTGTCCGTCTTTTATTTGTAAATTAAGGCATAGTTGATTTATATAGCAAATAAAGGTTTGATTTGTGTATGTATCTGATGTAATTAAATCTGTCTAATTTTTTAACCAGGACTATCAATTGATGAGTTTTCAAGGAAAAAATTGGACTTGACAGCAGAAGAGCTTTCTGAGGAGAAGGCTTTGGCTTACTCATGCCTCTCTTAG
Protein sequence

>MS.gene004851.t1

MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVDAAFPLLKGVVATTDVVEGCTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKHAAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVEVSNVKNVIIWGNHSSSQYPDVNHATVKIASAEKPVRELVADDAWLNGEFIATVQQRGAAIIKARKLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTTRNGEWQIVQGLSIDEFSRKKLDLTAEELSEEKALAYSCLS