Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006366.t1 | XP_003592388.2 | 95.1 | 245 | 11 | 1 | 1 | 245 | 1 | 244 | 4.30E-126 | 460.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006366.t1 | Q9FLI1 | 45.3 | 172 | 84 | 2 | 76 | 237 | 2 | 173 | 3.9e-27 | 123.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006366.t1 | G7ID49 | 95.1 | 245 | 11 | 1 | 1 | 245 | 1 | 244 | 3.1e-126 | 460.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene006366.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene37667 | MS.gene006366 | PPI |
| MS.gene070993 | MS.gene006366 | PPI |
| MS.gene006365 | MS.gene006366 | PPI |
| MS.gene34423 | MS.gene006366 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006366.t1 | MTR_1g102370 | 95.102 | 245 | 11 | 1 | 1 | 245 | 1 | 244 | 4.21e-172 | 474 |
| MS.gene006366.t1 | MTR_0007s0380 | 52.193 | 228 | 99 | 6 | 21 | 243 | 16 | 238 | 5.53e-65 | 202 |
| MS.gene006366.t1 | MTR_0007s0330 | 40.223 | 179 | 97 | 3 | 72 | 244 | 14 | 188 | 3.84e-34 | 122 |
| MS.gene006366.t1 | MTR_0007s0330 | 41.615 | 161 | 87 | 2 | 72 | 226 | 2 | 161 | 1.24e-32 | 117 |
| MS.gene006366.t1 | MTR_1g102380 | 35.714 | 182 | 101 | 2 | 61 | 231 | 4 | 180 | 3.87e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006366.t1 | AT5G51780 | 46.328 | 177 | 79 | 5 | 74 | 237 | 23 | 196 | 6.94e-38 | 131 |
| MS.gene006366.t1 | AT5G51780 | 46.286 | 175 | 78 | 5 | 76 | 237 | 2 | 173 | 1.76e-37 | 130 |
| MS.gene006366.t1 | AT4G25400 | 44.444 | 171 | 88 | 3 | 70 | 237 | 19 | 185 | 3.17e-36 | 127 |
| MS.gene006366.t1 | AT4G25400 | 46.405 | 153 | 75 | 3 | 70 | 219 | 19 | 167 | 1.52e-33 | 120 |
| MS.gene006366.t1 | AT4G25410 | 43.636 | 165 | 83 | 4 | 80 | 234 | 47 | 211 | 3.08e-33 | 120 |
| MS.gene006366.t1 | AT5G51790 | 40.541 | 185 | 98 | 5 | 62 | 234 | 9 | 193 | 6.45e-32 | 116 |
| MS.gene006366.t1 | AT5G51790 | 40.541 | 185 | 98 | 5 | 62 | 234 | 74 | 258 | 2.31e-31 | 116 |
| MS.gene006366.t1 | AT5G51790 | 41.765 | 170 | 87 | 5 | 62 | 219 | 9 | 178 | 2.31e-30 | 112 |
| MS.gene006366.t1 | AT5G51790 | 41.765 | 170 | 87 | 5 | 62 | 219 | 74 | 243 | 9.86e-30 | 112 |
| MS.gene006366.t1 | AT1G62975 | 32.161 | 199 | 111 | 3 | 63 | 237 | 61 | 259 | 3.13e-24 | 97.8 |
| MS.gene006366.t1 | AT1G12540 | 35.196 | 179 | 101 | 5 | 76 | 240 | 75 | 252 | 5.50e-22 | 91.7 |
| MS.gene006366.t1 | AT1G62975 | 33.149 | 181 | 97 | 3 | 63 | 219 | 61 | 241 | 7.76e-22 | 91.3 |
Find 42 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCCCACTTTCTAAATTTC+TGG | 0.103688 | 1.3:+70215881 | MS.gene006366:CDS |
| GAAGAGGGAGAAGGGATCTA+AGG | 0.225427 | 1.3:-70215537 | None:intergenic |
| CTGGAACAACTGATGAAATA+TGG | 0.304748 | 1.3:+70215900 | MS.gene006366:CDS |
| TATTGAAGATAAGAAGAAAA+TGG | 0.318828 | 1.3:+70215459 | MS.gene006366:CDS |
| TCTATATAAAATAATTTGTT+TGG | 0.336326 | 1.3:-70215406 | None:intergenic |
| ATAAGAAGAAAATGGTTCAT+AGG | 0.350975 | 1.3:+70215467 | MS.gene006366:CDS |
| ACTAAATGATGGATTCATAT+TGG | 0.351963 | 1.3:-70216140 | None:intergenic |
| AAATCACTTGAAGAAGAATA+TGG | 0.357120 | 1.3:+70215707 | MS.gene006366:CDS |
| TATATAGATCGTACAAATCA+TGG | 0.422086 | 1.3:+70215421 | MS.gene006366:CDS |
| TACTAGTACTTGCTTTATAG+AGG | 0.425326 | 1.3:+70215854 | MS.gene006366:CDS |
| GTGGTTGAGACTATTAGATA+AGG | 0.433605 | 1.3:-70215284 | None:intergenic |
| TGGAAGAACTAAGTTACAAA+AGG | 0.434554 | 1.3:+70215727 | MS.gene006366:CDS |
| ATACCTCACACTGCACACTA+TGG | 0.439476 | 1.3:-70215971 | None:intergenic |
| CCACAGTTCTATTATTCTTG+TGG | 0.455790 | 1.3:-70215818 | None:intergenic |
| ATATAAAATAATTTGTTTGG+TGG | 0.460635 | 1.3:-70215403 | None:intergenic |
| AAGAGGGAGAAGGGATCTAA+GGG | 0.467872 | 1.3:-70215536 | None:intergenic |
| TAATCTGAACAACTAAATGA+TGG | 0.468144 | 1.3:-70216151 | None:intergenic |
| TAAGAAGAAAATGGTTCATA+GGG | 0.475156 | 1.3:+70215468 | MS.gene006366:CDS |
| TAGATCTAACTGAGCTAAGA+AGG | 0.478104 | 1.3:+70216110 | MS.gene006366:CDS |
| TTTACAACGATGCAATGAAC+TGG | 0.483997 | 1.3:+70215255 | MS.gene006366:CDS |
| AGTTCATTGCATCGTTGTAA+AGG | 0.484172 | 1.3:-70215253 | None:intergenic |
| AAAATAATTTGTTTGGTGGC+CGG | 0.487549 | 1.3:-70215399 | None:intergenic |
| TGGTTGAGACTATTAGATAA+GGG | 0.494385 | 1.3:-70215283 | None:intergenic |
| GTTCCAGAAATTTAGAAAGT+GGG | 0.518377 | 1.3:-70215884 | None:intergenic |
| TTTATCTGATCAAATGAATG+AGG | 0.525114 | 1.3:+70215671 | MS.gene006366:CDS |
| TGTTCCAGAAATTTAGAAAG+TGG | 0.525663 | 1.3:-70215885 | None:intergenic |
| CACAAGAATAATAGAACTGT+GGG | 0.526811 | 1.3:+70215819 | MS.gene006366:CDS |
| GGAAGAACTAAGTTACAAAA+GGG | 0.531740 | 1.3:+70215728 | MS.gene006366:CDS |
| GAAGCAAAGGAGACAAGAAA+TGG | 0.533586 | 1.3:+70215498 | MS.gene006366:CDS |
| TCTCCCTCTTCACTTCATCA+AGG | 0.533778 | 1.3:+70215549 | MS.gene006366:CDS |
| TCACCTTGATGAAGTGAAGA+GGG | 0.538188 | 1.3:-70215552 | None:intergenic |
| GATGAAGTGAAGAGGGAGAA+GGG | 0.546212 | 1.3:-70215545 | None:intergenic |
| CCCACAACATCACAAAATCT+CGG | 0.553306 | 1.3:+70215309 | MS.gene006366:CDS |
| AGTACTTGCTTTATAGAGGA+AGG | 0.558027 | 1.3:+70215858 | MS.gene006366:CDS |
| TTCCAGAAATTTAGAAAGTG+GGG | 0.570258 | 1.3:-70215883 | None:intergenic |
| ATAGGGAAATTGAGAAGCAA+AGG | 0.575199 | 1.3:+70215485 | MS.gene006366:CDS |
| TGATGAAGTGAAGAGGGAGA+AGG | 0.577558 | 1.3:-70215546 | None:intergenic |
| CTCAGTTAGATCTACACTGT+CGG | 0.580625 | 1.3:-70216101 | None:intergenic |
| TTCACCTTGATGAAGTGAAG+AGG | 0.603402 | 1.3:-70215553 | None:intergenic |
| CCACAAGAATAATAGAACTG+TGG | 0.617517 | 1.3:+70215818 | MS.gene006366:CDS |
| ACTCCATAGTGTGCAGTGTG+AGG | 0.655288 | 1.3:+70215968 | MS.gene006366:CDS |
| TAGTGAAACTACAAGAAACA+TGG | 0.661078 | 1.3:-70215794 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTATATAAAATAATTTGTT+TGG | - | chr1.3:70215409-70215428 | None:intergenic | 10.0% |
| !! | TTAAAAAAATAGATACTAAC+CGG | + | chr1.3:70215380-70215399 | MS.gene006366:CDS | 15.0% |
| !!! | ATATAAAATAATTTGTTTGG+TGG | - | chr1.3:70215406-70215425 | None:intergenic | 15.0% |
| !!! | TTTAATCATTAAAGTTTTCT+AGG | + | chr1.3:70215591-70215610 | MS.gene006366:intron | 15.0% |
| !! | TATCATCTGTTATAACTTTA+GGG | + | chr1.3:70215615-70215634 | MS.gene006366:intron | 20.0% |
| !! | TATTGAAGATAAGAAGAAAA+TGG | + | chr1.3:70215459-70215478 | MS.gene006366:CDS | 20.0% |
| !! | TTATCATCTGTTATAACTTT+AGG | + | chr1.3:70215614-70215633 | MS.gene006366:intron | 20.0% |
| !!! | AAACATGGCTTTTATTTTTA+AGG | - | chr1.3:70215782-70215801 | None:intergenic | 20.0% |
| !!! | TATTTATTGTTTCTCTAACA+GGG | + | chr1.3:70215639-70215658 | MS.gene006366:intron | 20.0% |
| !!! | TTATTTATTGTTTCTCTAAC+AGG | + | chr1.3:70215638-70215657 | MS.gene006366:intron | 20.0% |
| ! | AAATCACTTGAAGAAGAATA+TGG | + | chr1.3:70215707-70215726 | MS.gene006366:CDS | 25.0% |
| ! | ATAAGAAGAAAATGGTTCAT+AGG | + | chr1.3:70215467-70215486 | MS.gene006366:CDS | 25.0% |
| ! | TAAGAAGAAAATGGTTCATA+GGG | + | chr1.3:70215468-70215487 | MS.gene006366:CDS | 25.0% |
| ! | TAATCTGAACAACTAAATGA+TGG | - | chr1.3:70216154-70216173 | None:intergenic | 25.0% |
| ! | TATATAGATCGTACAAATCA+TGG | + | chr1.3:70215421-70215440 | MS.gene006366:CDS | 25.0% |
| ! | TTTATCTGATCAAATGAATG+AGG | + | chr1.3:70215671-70215690 | MS.gene006366:CDS | 25.0% |
| !!! | ACTAAATGATGGATTCATAT+TGG | - | chr1.3:70216143-70216162 | None:intergenic | 25.0% |
| CACAAGAATAATAGAACTGT+GGG | + | chr1.3:70215819-70215838 | MS.gene006366:CDS | 30.0% | |
| GGAAGAACTAAGTTACAAAA+GGG | + | chr1.3:70215728-70215747 | MS.gene006366:CDS | 30.0% | |
| GTTCCAGAAATTTAGAAAGT+GGG | - | chr1.3:70215887-70215906 | None:intergenic | 30.0% | |
| TACTAGTACTTGCTTTATAG+AGG | + | chr1.3:70215854-70215873 | MS.gene006366:CDS | 30.0% | |
| TAGTGAAACTACAAGAAACA+TGG | - | chr1.3:70215797-70215816 | None:intergenic | 30.0% | |
| TGGAAGAACTAAGTTACAAA+AGG | + | chr1.3:70215727-70215746 | MS.gene006366:CDS | 30.0% | |
| TGGTTGAGACTATTAGATAA+GGG | - | chr1.3:70215286-70215305 | None:intergenic | 30.0% | |
| TGTTCCAGAAATTTAGAAAG+TGG | - | chr1.3:70215888-70215907 | None:intergenic | 30.0% | |
| TTCCAGAAATTTAGAAAGTG+GGG | - | chr1.3:70215886-70215905 | None:intergenic | 30.0% | |
| ! | TGCTTTTCTATTCAAGTCAA+TGG | + | chr1.3:70215939-70215958 | MS.gene006366:CDS | 30.0% |
| !! | AAAATAATTTGTTTGGTGGC+CGG | - | chr1.3:70215402-70215421 | None:intergenic | 30.0% |
| !!! | TGGCTTTTATTTTTAAGGCT+AGG | - | chr1.3:70215777-70215796 | None:intergenic | 30.0% |
| AGTACTTGCTTTATAGAGGA+AGG | + | chr1.3:70215858-70215877 | MS.gene006366:CDS | 35.0% | |
| AGTTCATTGCATCGTTGTAA+AGG | - | chr1.3:70215256-70215275 | None:intergenic | 35.0% | |
| ATAGGGAAATTGAGAAGCAA+AGG | + | chr1.3:70215485-70215504 | MS.gene006366:CDS | 35.0% | |
| CCACAAGAATAATAGAACTG+TGG | + | chr1.3:70215818-70215837 | MS.gene006366:CDS | 35.0% | |
| CCACAGTTCTATTATTCTTG+TGG | - | chr1.3:70215821-70215840 | None:intergenic | 35.0% | |
| CTGGAACAACTGATGAAATA+TGG | + | chr1.3:70215900-70215919 | MS.gene006366:CDS | 35.0% | |
| GTGGTTGAGACTATTAGATA+AGG | - | chr1.3:70215287-70215306 | None:intergenic | 35.0% | |
| TAGATCTAACTGAGCTAAGA+AGG | + | chr1.3:70216110-70216129 | MS.gene006366:CDS | 35.0% | |
| TTTACAACGATGCAATGAAC+TGG | + | chr1.3:70215255-70215274 | MS.gene006366:CDS | 35.0% | |
| ! | TATTGAGCTTGATGATTCAC+AGG | + | chr1.3:70216069-70216088 | MS.gene006366:intron | 35.0% |
| CCCACAACATCACAAAATCT+CGG | + | chr1.3:70215309-70215328 | MS.gene006366:CDS | 40.0% | |
| CTCAGTTAGATCTACACTGT+CGG | - | chr1.3:70216104-70216123 | None:intergenic | 40.0% | |
| GAAGCAAAGGAGACAAGAAA+TGG | + | chr1.3:70215498-70215517 | MS.gene006366:CDS | 40.0% | |
| TCACCTTGATGAAGTGAAGA+GGG | - | chr1.3:70215555-70215574 | None:intergenic | 40.0% | |
| TGCCCCACTTTCTAAATTTC+TGG | + | chr1.3:70215881-70215900 | MS.gene006366:CDS | 40.0% | |
| TTCACCTTGATGAAGTGAAG+AGG | - | chr1.3:70215556-70215575 | None:intergenic | 40.0% | |
| ! | AGATTTTGTGATGTTGTGGG+TGG | - | chr1.3:70215309-70215328 | None:intergenic | 40.0% |
| ! | CCGAGATTTTGTGATGTTGT+GGG | - | chr1.3:70215312-70215331 | None:intergenic | 40.0% |
| ! | TCCGAGATTTTGTGATGTTG+TGG | - | chr1.3:70215313-70215332 | None:intergenic | 40.0% |
| AAGAGGGAGAAGGGATCTAA+GGG | - | chr1.3:70215539-70215558 | None:intergenic | 45.0% | |
| ATACCTCACACTGCACACTA+TGG | - | chr1.3:70215974-70215993 | None:intergenic | 45.0% | |
| GATGAAGTGAAGAGGGAGAA+GGG | - | chr1.3:70215548-70215567 | None:intergenic | 45.0% | |
| TCTCCCTCTTCACTTCATCA+AGG | + | chr1.3:70215549-70215568 | MS.gene006366:CDS | 45.0% | |
| TGATGAAGTGAAGAGGGAGA+AGG | - | chr1.3:70215549-70215568 | None:intergenic | 45.0% | |
| ! | TTTTGTGATGTTGTGGGTGG+TGG | - | chr1.3:70215306-70215325 | None:intergenic | 45.0% |
| ACTCCATAGTGTGCAGTGTG+AGG | + | chr1.3:70215968-70215987 | MS.gene006366:CDS | 50.0% | |
| GAAGAGGGAGAAGGGATCTA+AGG | - | chr1.3:70215540-70215559 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 70215247 | 70216174 | 70215247 | ID=MS.gene006366 |
| chr1.3 | mRNA | 70215247 | 70216174 | 70215247 | ID=MS.gene006366.t1;Parent=MS.gene006366 |
| chr1.3 | exon | 70215247 | 70215570 | 70215247 | ID=MS.gene006366.t1.exon1;Parent=MS.gene006366.t1 |
| chr1.3 | CDS | 70215247 | 70215570 | 70215247 | ID=cds.MS.gene006366.t1;Parent=MS.gene006366.t1 |
| chr1.3 | exon | 70215660 | 70215989 | 70215660 | ID=MS.gene006366.t1.exon2;Parent=MS.gene006366.t1 |
| chr1.3 | CDS | 70215660 | 70215989 | 70215660 | ID=cds.MS.gene006366.t1;Parent=MS.gene006366.t1 |
| chr1.3 | exon | 70216091 | 70216174 | 70216091 | ID=MS.gene006366.t1.exon3;Parent=MS.gene006366.t1 |
| chr1.3 | CDS | 70216091 | 70216174 | 70216091 | ID=cds.MS.gene006366.t1;Parent=MS.gene006366.t1 |
| Gene Sequence |
| Protein sequence |
