Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070993.t1 | XP_003592389.1 | 93.5 | 168 | 11 | 0 | 1 | 168 | 1 | 168 | 1.00E-78 | 302.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070993.t1 | Q9FLI1 | 35.2 | 159 | 92 | 3 | 19 | 170 | 2 | 156 | 1.1e-17 | 91.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070993.t1 | G7ID50 | 93.5 | 168 | 11 | 0 | 1 | 168 | 1 | 168 | 7.5e-79 | 302.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene070993.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene070993 | MS.gene37668 | PPI |
| MS.gene070993 | MS.gene34422 | PPI |
| MS.gene070993 | MS.gene006366 | PPI |
| MS.gene070993 | MS.gene070992 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070993.t1 | MTR_1g102380 | 93.452 | 168 | 11 | 0 | 1 | 168 | 1 | 168 | 1.93e-112 | 317 |
| MS.gene070993.t1 | MTR_0007s0330 | 38.961 | 154 | 91 | 2 | 17 | 169 | 4 | 155 | 4.74e-36 | 123 |
| MS.gene070993.t1 | MTR_0007s0330 | 39.474 | 152 | 89 | 2 | 17 | 167 | 16 | 165 | 4.84e-35 | 122 |
| MS.gene070993.t1 | MTR_1g102370 | 41.447 | 152 | 83 | 2 | 17 | 168 | 73 | 218 | 2.29e-32 | 116 |
| MS.gene070993.t1 | MTR_0007s0380 | 37.580 | 157 | 88 | 3 | 15 | 169 | 67 | 215 | 2.08e-30 | 110 |
| MS.gene070993.t1 | MTR_2g013920 | 28.378 | 148 | 102 | 2 | 23 | 170 | 12 | 155 | 1.27e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070993.t1 | AT5G51780 | 35.758 | 165 | 95 | 3 | 13 | 170 | 19 | 179 | 2.81e-26 | 99.4 |
| MS.gene070993.t1 | AT5G51780 | 35.220 | 159 | 92 | 3 | 19 | 170 | 2 | 156 | 2.68e-24 | 93.6 |
| MS.gene070993.t1 | AT5G51790 | 32.716 | 162 | 91 | 5 | 18 | 169 | 26 | 179 | 5.47e-23 | 90.9 |
| MS.gene070993.t1 | AT5G51790 | 32.919 | 161 | 90 | 5 | 18 | 168 | 26 | 178 | 5.85e-23 | 90.5 |
| MS.gene070993.t1 | AT1G12540 | 38.554 | 166 | 89 | 7 | 15 | 170 | 71 | 233 | 7.40e-23 | 91.7 |
| MS.gene070993.t1 | AT5G51790 | 32.919 | 161 | 90 | 5 | 18 | 168 | 91 | 243 | 1.59e-22 | 90.9 |
| MS.gene070993.t1 | AT5G51790 | 32.716 | 162 | 91 | 5 | 18 | 169 | 91 | 244 | 1.66e-22 | 90.9 |
| MS.gene070993.t1 | AT4G25410 | 32.692 | 156 | 87 | 3 | 24 | 168 | 48 | 196 | 2.92e-22 | 89.4 |
| MS.gene070993.t1 | AT4G25400 | 31.646 | 158 | 101 | 3 | 13 | 170 | 19 | 169 | 4.24e-22 | 88.2 |
| MS.gene070993.t1 | AT4G25400 | 32.051 | 156 | 99 | 3 | 13 | 168 | 19 | 167 | 7.31e-22 | 87.4 |
| MS.gene070993.t1 | AT1G62975 | 34.302 | 172 | 95 | 4 | 15 | 168 | 70 | 241 | 2.67e-20 | 84.7 |
| MS.gene070993.t1 | AT1G62975 | 34.302 | 172 | 95 | 4 | 15 | 168 | 70 | 241 | 4.00e-20 | 84.7 |
Find 29 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACGTTTATCAGATTTAA+TGG | 0.200332 | 1.4:-79158814 | MS.gene070993:CDS |
| ACACACTTCACTACACTTGT+AGG | 0.335290 | 1.4:+79158900 | None:intergenic |
| TGTGTTACCAACCAAGTAAA+TGG | 0.368730 | 1.4:-79158756 | MS.gene070993:CDS |
| GTCAAGATTGATCTAATTCC+AGG | 0.380052 | 1.4:-79158879 | MS.gene070993:CDS |
| TTTATGCTGTTTCAGATCTT+CGG | 0.390845 | 1.4:+79159311 | None:intergenic |
| GAGAGATAAGATCAAAGAAA+TGG | 0.392291 | 1.4:-79158967 | MS.gene070993:CDS |
| AGTTCAAGTAGGGTTGAAAC+TGG | 0.407760 | 1.4:-79158942 | MS.gene070993:CDS |
| AAGACCTTGATGAGCTCAAA+AGG | 0.412689 | 1.4:+79159180 | None:intergenic |
| GAAATATCTTGCCATTTACT+TGG | 0.419250 | 1.4:+79158745 | None:intergenic |
| TATCTTGCCATTTACTTGGT+TGG | 0.419948 | 1.4:+79158749 | None:intergenic |
| AAGAAATGGTTAGTTCAAGT+AGG | 0.512409 | 1.4:-79158953 | MS.gene070993:CDS |
| AGAAATGGTTAGTTCAAGTA+GGG | 0.521680 | 1.4:-79158952 | MS.gene070993:CDS |
| ATAACAATTTCCACCCCACC+TGG | 0.524411 | 1.4:+79158861 | None:intergenic |
| GTGGAAATTGTTATATGTAG+TGG | 0.547521 | 1.4:-79158852 | MS.gene070993:CDS |
| ATGGAAATAATACTTCAAGA+AGG | 0.552508 | 1.4:-79158795 | MS.gene070993:CDS |
| TGATCTAATTCCAGGTGGGG+TGG | 0.577334 | 1.4:-79158871 | MS.gene070993:CDS |
| AGACCTTGATGAGCTCAAAA+GGG | 0.578199 | 1.4:+79159181 | None:intergenic |
| GCATAAAATATTAACTACAT+CGG | 0.605079 | 1.4:-79159295 | MS.gene070993:CDS |
| ATTTCATACTATTAAATCTG+AGG | 0.612912 | 1.4:-79158727 | MS.gene070993:intron |
| GATTGATCTAATTCCAGGTG+GGG | 0.621637 | 1.4:-79158874 | MS.gene070993:CDS |
| AGATTGATCTAATTCCAGGT+GGG | 0.625836 | 1.4:-79158875 | MS.gene070993:CDS |
| GCTCAAAAGGGAGTGAAGAA+CGG | 0.633531 | 1.4:+79159193 | None:intergenic |
| GTAGGGAAGTTGAAAAGCAA+AGG | 0.634358 | 1.4:-79159248 | MS.gene070993:CDS |
| AAAAGGGAGTGAAGAACGGA+GGG | 0.640895 | 1.4:+79159197 | None:intergenic |
| CGTTCAGTATCAGATCACAT+AGG | 0.655166 | 1.4:-79159047 | MS.gene070993:CDS |
| AGTATCAGATCACATAGGTG+AGG | 0.667408 | 1.4:-79159042 | MS.gene070993:CDS |
| CAAAAGGGAGTGAAGAACGG+AGG | 0.674543 | 1.4:+79159196 | None:intergenic |
| GGGAAGTTGAAAAGCAAAGG+AGG | 0.678696 | 1.4:-79159245 | MS.gene070993:CDS |
| AAGATTGATCTAATTCCAGG+TGG | 0.682027 | 1.4:-79158876 | MS.gene070993:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATATATATATGTTATG+AGG | - | chr1.4:79158585-79158604 | MS.gene070993:intron | 10.0% |
| !!! | GAATTTTGTAATTTTTTTTT+AGG | - | chr1.4:79159304-79159323 | MS.gene070993:CDS | 10.0% |
| !! | AACTTAATAATTAATTAGCT+AGG | + | chr1.4:79158265-79158284 | None:intergenic | 15.0% |
| !!! | ATTATTGTTGTTGAATATAT+AGG | - | chr1.4:79158400-79158419 | MS.gene070993:intron | 15.0% |
| !!! | TTTGTAATTTTTTTTTAGGT+AGG | - | chr1.4:79159308-79159327 | MS.gene070993:CDS | 15.0% |
| !! | ATTTCATACTATTAAATCTG+AGG | - | chr1.4:79158233-79158252 | MS.gene070993:intron | 20.0% |
| !! | GCATAAAATATTAACTACAT+CGG | - | chr1.4:79157665-79157684 | MS.gene070993:intron | 20.0% |
| !! | TTAAAGATATAGAGAACAAA+TGG | - | chr1.4:79158947-79158966 | MS.gene070993:CDS | 20.0% |
| !!! | TAATTTTCTGCTTCAAAATT+TGG | + | chr1.4:79157957-79157976 | None:intergenic | 20.0% |
| !!! | TCATAACATCTTTGATTTTA+GGG | - | chr1.4:79157886-79157905 | MS.gene070993:intron | 20.0% |
| !!! | TCATGTTGATAAATAATAAG+AGG | + | chr1.4:79157826-79157845 | None:intergenic | 20.0% |
| !!! | TTCATAACATCTTTGATTTT+AGG | - | chr1.4:79157885-79157904 | MS.gene070993:intron | 20.0% |
| ! | AATTCAAATCTACTCAGATA+CGG | + | chr1.4:79159076-79159095 | None:intergenic | 25.0% |
| ! | ATGGAAATAATACTTCAAGA+AGG | - | chr1.4:79158165-79158184 | MS.gene070993:intron | 25.0% |
| ! | CAAGATAAAATCAAGATTTG+TGG | - | chr1.4:79159165-79159184 | MS.gene070993:intron | 25.0% |
| ! | TAATGGTATATAACATAGAC+TGG | + | chr1.4:79158895-79158914 | None:intergenic | 25.0% |
| ! | TGCAAATTAAGACAGTTTAA+TGG | + | chr1.4:79158912-79158931 | None:intergenic | 25.0% |
| ! | TTCACGTTTATCAGATTTAA+TGG | - | chr1.4:79158146-79158165 | MS.gene070993:intron | 25.0% |
| !! | ATTGTTGTTGAATATATAGG+TGG | - | chr1.4:79158403-79158422 | MS.gene070993:intron | 25.0% |
| AATTACATTCAGATCAACCA+AGG | + | chr1.4:79158746-79158765 | None:intergenic | 30.0% | |
| ATGAGAAAAAGATGATTCGT+AGG | - | chr1.4:79157694-79157713 | MS.gene070993:intron | 30.0% | |
| CTCTATATCTTTAAGCAACA+AGG | + | chr1.4:79158941-79158960 | None:intergenic | 30.0% | |
| GAGAGATAAGATCAAAGAAA+TGG | - | chr1.4:79157993-79158012 | MS.gene070993:intron | 30.0% | |
| GTAGTTGCATGTAAATCTAA+TGG | + | chr1.4:79158831-79158850 | None:intergenic | 30.0% | |
| GTGGAAATTGTTATATGTAG+TGG | - | chr1.4:79158108-79158127 | MS.gene070993:intron | 30.0% | |
| TAGAGAACAAATGGATAGAA+TGG | - | chr1.4:79158956-79158975 | MS.gene070993:CDS | 30.0% | |
| TGAGAAAAAGATGATTCGTA+GGG | - | chr1.4:79157695-79157714 | MS.gene070993:intron | 30.0% | |
| ! | AAGAAATGGTTAGTTCAAGT+AGG | - | chr1.4:79158007-79158026 | MS.gene070993:intron | 30.0% |
| ! | AGAAATGGTTAGTTCAAGTA+GGG | - | chr1.4:79158008-79158027 | MS.gene070993:intron | 30.0% |
| ! | ATCACGCAATTTTATTGTCA+AGG | - | chr1.4:79158471-79158490 | MS.gene070993:intron | 30.0% |
| ! | GAAATATCTTGCCATTTACT+TGG | + | chr1.4:79158218-79158237 | None:intergenic | 30.0% |
| ! | TAAGGATTCTCAATTTTCCT+TGG | - | chr1.4:79158726-79158745 | MS.gene070993:intron | 30.0% |
| !! | TGTTATATGTAGTGGTTTTG+AGG | - | chr1.4:79158116-79158135 | MS.gene070993:intron | 30.0% |
| !! | TTTATGCTGTTTCAGATCTT+CGG | + | chr1.4:79157652-79157671 | None:intergenic | 30.0% |
| AACATTGGAAGTCAGTTTAG+TGG | + | chr1.4:79159132-79159151 | None:intergenic | 35.0% | |
| AAGATTGATCTAATTCCAGG+TGG | - | chr1.4:79158084-79158103 | MS.gene070993:intron | 35.0% | |
| ACTCAGATACGGTTGTAAAA+TGG | + | chr1.4:79159065-79159084 | None:intergenic | 35.0% | |
| AGATTGATCTAATTCCAGGT+GGG | - | chr1.4:79158085-79158104 | MS.gene070993:intron | 35.0% | |
| GTAAATGTGTGACTGTGATA+AGG | - | chr1.4:79158708-79158727 | MS.gene070993:intron | 35.0% | |
| GTCAAGATTGATCTAATTCC+AGG | - | chr1.4:79158081-79158100 | MS.gene070993:intron | 35.0% | |
| TACCTTCGTTCATCAGATTA+AGG | + | chr1.4:79158804-79158823 | None:intergenic | 35.0% | |
| TGTCTCAAGTTTGACAACAT+TGG | + | chr1.4:79159147-79159166 | None:intergenic | 35.0% | |
| TGTGTTACCAACCAAGTAAA+TGG | - | chr1.4:79158204-79158223 | MS.gene070993:intron | 35.0% | |
| ! | GTATATAACATAGACTGGTC+AGG | + | chr1.4:79158890-79158909 | None:intergenic | 35.0% |
| ! | TATCTTGCCATTTACTTGGT+TGG | + | chr1.4:79158214-79158233 | None:intergenic | 35.0% |
| !! | TCTAGGGTATTTCTGAGTTT+TGG | - | chr1.4:79158337-79158356 | MS.gene070993:intron | 35.0% |
| AAGACCTTGATGAGCTCAAA+AGG | + | chr1.4:79157783-79157802 | None:intergenic | 40.0% | |
| ACACACTTCACTACACTTGT+AGG | + | chr1.4:79158063-79158082 | None:intergenic | 40.0% | |
| AGACCTTGATGAGCTCAAAA+GGG | + | chr1.4:79157782-79157801 | None:intergenic | 40.0% | |
| AGTATCAGATCACATAGGTG+AGG | - | chr1.4:79157918-79157937 | MS.gene070993:intron | 40.0% | |
| AGTTCAAGTAGGGTTGAAAC+TGG | - | chr1.4:79158018-79158037 | MS.gene070993:intron | 40.0% | |
| CGTTCAGTATCAGATCACAT+AGG | - | chr1.4:79157913-79157932 | MS.gene070993:intron | 40.0% | |
| CTACTAACTCATCCACAACT+TGG | - | chr1.4:79158864-79158883 | MS.gene070993:CDS | 40.0% | |
| GATTGATCTAATTCCAGGTG+GGG | - | chr1.4:79158086-79158105 | MS.gene070993:intron | 40.0% | |
| GTAGGGAAGTTGAAAAGCAA+AGG | - | chr1.4:79157712-79157731 | MS.gene070993:intron | 40.0% | |
| TACGATCTAGCTAGAGTCTA+GGG | - | chr1.4:79158321-79158340 | MS.gene070993:intron | 40.0% | |
| TGCCTTAATCTGATGAACGA+AGG | - | chr1.4:79158799-79158818 | MS.gene070993:CDS | 40.0% | |
| TGCTCAGCTGCAAAATAAAC+TGG | - | chr1.4:79158448-79158467 | MS.gene070993:intron | 40.0% | |
| TTACGATCTAGCTAGAGTCT+AGG | - | chr1.4:79158320-79158339 | MS.gene070993:intron | 40.0% | |
| TTTGCAGCTGAGCAAGATTA+AGG | + | chr1.4:79158442-79158461 | None:intergenic | 40.0% | |
| !! | AATTAACTTGTACCTGTGCG+TGG | - | chr1.4:79158663-79158682 | MS.gene070993:intron | 40.0% |
| !!! | TTTTTTTTGTCTCCACGCAC+AGG | + | chr1.4:79158678-79158697 | None:intergenic | 40.0% |
| AAAAGGGAGTGAAGAACGGA+GGG | + | chr1.4:79157766-79157785 | None:intergenic | 45.0% | |
| ATAACAATTTCCACCCCACC+TGG | + | chr1.4:79158102-79158121 | None:intergenic | 45.0% | |
| GCTCAAAAGGGAGTGAAGAA+CGG | + | chr1.4:79157770-79157789 | None:intergenic | 45.0% | |
| GGGAAGTTGAAAAGCAAAGG+AGG | - | chr1.4:79157715-79157734 | MS.gene070993:intron | 45.0% | |
| ! | ACTCCCTTTTGAGCTCATCA+AGG | - | chr1.4:79157776-79157795 | MS.gene070993:intron | 45.0% |
| ! | GTGGCATTCACGTTAGTTTG+TGG | - | chr1.4:79159184-79159203 | MS.gene070993:intron | 45.0% |
| CAAAAGGGAGTGAAGAACGG+AGG | + | chr1.4:79157767-79157786 | None:intergenic | 50.0% | |
| TGATCTAATTCCAGGTGGGG+TGG | - | chr1.4:79158089-79158108 | MS.gene070993:intron | 50.0% | |
| ! | AGACTGGTCAGGCCAAGTTG+TGG | + | chr1.4:79158879-79158898 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 79157645 | 79159337 | 79157645 | ID=MS.gene070993 |
| chr1.4 | mRNA | 79157645 | 79159337 | 79157645 | ID=MS.gene070993.t1;Parent=MS.gene070993 |
| chr1.4 | exon | 79159185 | 79159337 | 79159185 | ID=MS.gene070993.t1.exon1;Parent=MS.gene070993.t1 |
| chr1.4 | CDS | 79159185 | 79159337 | 79159185 | ID=cds.MS.gene070993.t1;Parent=MS.gene070993.t1 |
| chr1.4 | exon | 79158728 | 79159075 | 79158728 | ID=MS.gene070993.t1.exon2;Parent=MS.gene070993.t1 |
| chr1.4 | CDS | 79158728 | 79159075 | 79158728 | ID=cds.MS.gene070993.t1;Parent=MS.gene070993.t1 |
| chr1.4 | exon | 79157645 | 79157656 | 79157645 | ID=MS.gene070993.t1.exon3;Parent=MS.gene070993.t1 |
| chr1.4 | CDS | 79157645 | 79157656 | 79157645 | ID=cds.MS.gene070993.t1;Parent=MS.gene070993.t1 |
| Gene Sequence |
| Protein sequence |
