Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37667.t1 | XP_003592389.1 | 94.6 | 167 | 9 | 0 | 1 | 167 | 1 | 167 | 2.10E-79 | 305.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37667.t1 | Q9FLI1 | 34.6 | 156 | 91 | 3 | 19 | 167 | 2 | 153 | 1.5e-17 | 90.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37667.t1 | G7ID50 | 94.6 | 167 | 9 | 0 | 1 | 167 | 1 | 167 | 1.5e-79 | 305.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene37667.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene37667 | MS.gene006366 | PPI |
| MS.gene34422 | MS.gene37667 | PPI |
| MS.gene37667 | MS.gene37668 | PPI |
| MS.gene070992 | MS.gene37667 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37667.t1 | MTR_1g102380 | 94.611 | 167 | 9 | 0 | 1 | 167 | 1 | 167 | 2.68e-112 | 317 |
| MS.gene37667.t1 | MTR_0007s0330 | 38.816 | 152 | 90 | 2 | 17 | 167 | 4 | 153 | 4.44e-36 | 123 |
| MS.gene37667.t1 | MTR_0007s0330 | 38.816 | 152 | 90 | 2 | 17 | 167 | 16 | 165 | 1.28e-35 | 123 |
| MS.gene37667.t1 | MTR_1g102370 | 41.558 | 154 | 78 | 2 | 17 | 167 | 73 | 217 | 1.20e-31 | 114 |
| MS.gene37667.t1 | MTR_0007s0380 | 38.926 | 149 | 85 | 3 | 19 | 167 | 71 | 213 | 1.41e-29 | 108 |
| MS.gene37667.t1 | MTR_2g013920 | 27.703 | 148 | 103 | 2 | 23 | 170 | 12 | 155 | 3.08e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37667.t1 | AT5G51780 | 35.185 | 162 | 94 | 3 | 13 | 167 | 19 | 176 | 1.34e-25 | 97.4 |
| MS.gene37667.t1 | AT5G51780 | 34.615 | 156 | 91 | 3 | 19 | 167 | 2 | 153 | 1.44e-23 | 91.7 |
| MS.gene37667.t1 | AT1G12540 | 37.500 | 160 | 87 | 7 | 18 | 167 | 74 | 230 | 1.21e-21 | 88.6 |
| MS.gene37667.t1 | AT4G25410 | 32.432 | 148 | 96 | 2 | 24 | 167 | 48 | 195 | 3.77e-21 | 86.7 |
| MS.gene37667.t1 | AT5G51790 | 31.818 | 154 | 99 | 3 | 18 | 167 | 26 | 177 | 1.31e-20 | 84.3 |
| MS.gene37667.t1 | AT5G51790 | 31.818 | 154 | 99 | 3 | 18 | 167 | 26 | 177 | 1.41e-20 | 84.7 |
| MS.gene37667.t1 | AT4G25400 | 32.215 | 149 | 94 | 3 | 19 | 167 | 25 | 166 | 2.07e-20 | 83.6 |
| MS.gene37667.t1 | AT1G62975 | 33.918 | 171 | 95 | 4 | 15 | 167 | 70 | 240 | 2.40e-20 | 84.7 |
| MS.gene37667.t1 | AT4G25400 | 32.215 | 149 | 94 | 3 | 19 | 167 | 25 | 166 | 2.50e-20 | 83.6 |
| MS.gene37667.t1 | AT1G62975 | 33.918 | 171 | 95 | 4 | 15 | 167 | 70 | 240 | 3.57e-20 | 84.7 |
| MS.gene37667.t1 | AT5G51790 | 31.818 | 154 | 99 | 3 | 18 | 167 | 91 | 242 | 5.72e-20 | 84.0 |
| MS.gene37667.t1 | AT5G51790 | 31.818 | 154 | 99 | 3 | 18 | 167 | 91 | 242 | 6.24e-20 | 84.3 |
Find 30 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACGTTTATCAGATTTAA+TGG | 0.200332 | 1.1:-73381633 | MS.gene37667:CDS |
| TGTGTTACCAACCAAGTAAA+TGG | 0.368730 | 1.1:-73381575 | MS.gene37667:CDS |
| GTCAAGATTGATCTAATTCC+AGG | 0.375644 | 1.1:-73381698 | MS.gene37667:CDS |
| GAGAGATAAGATCAAAGAAA+TGG | 0.392291 | 1.1:-73381786 | MS.gene37667:CDS |
| AGTTCAAGTAGGGTTGAAAC+TGG | 0.407760 | 1.1:-73381761 | MS.gene37667:CDS |
| AAGACCTTGATGAGCTCAAA+AGG | 0.412689 | 1.1:+73382005 | None:intergenic |
| GAAATATCTTGCCATTTACT+TGG | 0.419250 | 1.1:+73381564 | None:intergenic |
| TATCTTGCCATTTACTTGGT+TGG | 0.419948 | 1.1:+73381568 | None:intergenic |
| ATAACAATTTCCACCCCTCC+TGG | 0.452997 | 1.1:+73381680 | None:intergenic |
| TTTATGCTGATTCAGATCTT+CGG | 0.455944 | 1.1:+73382136 | None:intergenic |
| TGTTATATGTAGTGGTTTCG+AGG | 0.477906 | 1.1:-73381663 | MS.gene37667:CDS |
| GTGGAAATTGTTATATGTAG+TGG | 0.497463 | 1.1:-73381671 | MS.gene37667:CDS |
| AAGAAATGGTTAGTTCAAGT+AGG | 0.512409 | 1.1:-73381772 | MS.gene37667:CDS |
| AGAAATGGTTAGTTCAAGTA+GGG | 0.521680 | 1.1:-73381771 | MS.gene37667:CDS |
| ATGGAAATAATACTTCAAGA+AGG | 0.552508 | 1.1:-73381614 | MS.gene37667:CDS |
| TTTATCATCATTGTCCGATG+TGG | 0.567491 | 1.1:+73382106 | None:intergenic |
| AGACCTTGATGAGCTCAAAA+GGG | 0.578199 | 1.1:+73382006 | None:intergenic |
| TGATCTAATTCCAGGAGGGG+TGG | 0.596836 | 1.1:-73381690 | MS.gene37667:CDS |
| ATTTCATACTGTTAAATCTG+AGG | 0.612912 | 1.1:-73381546 | MS.gene37667:intron |
| AGATTGATCTAATTCCAGGA+GGG | 0.618885 | 1.1:-73381694 | MS.gene37667:CDS |
| GATTGATCTAATTCCAGGAG+GGG | 0.624077 | 1.1:-73381693 | MS.gene37667:CDS |
| GCTCAAAAGGGAGTGAAGAA+CGG | 0.633531 | 1.1:+73382018 | None:intergenic |
| GTAGGGAAGTTGAAAAGCAA+AGG | 0.634358 | 1.1:-73382073 | MS.gene37667:CDS |
| AAAAGGGAGTGAAGAACGGA+GGG | 0.640895 | 1.1:+73382022 | None:intergenic |
| GCATAAAATATTAACCACAT+CGG | 0.653294 | 1.1:-73382120 | MS.gene37667:CDS |
| CGTTCAGTATCAGATCACAT+AGG | 0.655166 | 1.1:-73381866 | MS.gene37667:CDS |
| AGTATCAGATCACATAGGTG+AGG | 0.667408 | 1.1:-73381861 | MS.gene37667:CDS |
| CAAAAGGGAGTGAAGAACGG+AGG | 0.674543 | 1.1:+73382021 | None:intergenic |
| GGGAAGTTGAAAAGCAAAGG+AGG | 0.678696 | 1.1:-73382070 | MS.gene37667:CDS |
| AAGATTGATCTAATTCCAGG+AGG | 0.709912 | 1.1:-73381695 | MS.gene37667:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATATATATATGTTATG+AGG | - | chr1.1:73381407-73381426 | MS.gene37667:intron | 10.0% |
| !!! | GAATTTTGTAATTTTTTTTT+AGG | - | chr1.1:73382129-73382148 | MS.gene37667:CDS | 10.0% |
| !! | AACTTAATAATTAATTAGCT+AGG | + | chr1.1:73381139-73381158 | None:intergenic | 15.0% |
| !!! | ATTATTGTTGTTGAATATAT+AGG | - | chr1.1:73381272-73381291 | MS.gene37667:intron | 15.0% |
| !! | TTAAAGATATAGAGAACAAA+TGG | - | chr1.1:73381770-73381789 | MS.gene37667:CDS | 20.0% |
| !!! | TAATTTTCTGCTTCAAAATT+TGG | + | chr1.1:73380835-73380854 | None:intergenic | 20.0% |
| !!! | TCATGTTGATAAATAATAAG+AGG | + | chr1.1:73380700-73380719 | None:intergenic | 20.0% |
| !!! | TTTGTAATTTTTTTTTAGGG+AGG | - | chr1.1:73382133-73382152 | MS.gene37667:CDS | 20.0% |
| ! | AATTCAAATCTACTCAGATA+CGG | + | chr1.1:73381902-73381921 | None:intergenic | 25.0% |
| ! | ATGATAAAAAGATGATTCGT+AGG | - | chr1.1:73380566-73380585 | MS.gene37667:intron | 25.0% |
| ! | ATGGAAATAATACTTCAAGA+AGG | - | chr1.1:73381043-73381062 | MS.gene37667:intron | 25.0% |
| ! | ATTTCATACTGTTAAATCTG+AGG | - | chr1.1:73381111-73381130 | MS.gene37667:intron | 25.0% |
| ! | CAAGATAAAATCAAGATTTG+TGG | - | chr1.1:73381990-73382009 | MS.gene37667:intron | 25.0% |
| ! | GCATAAAATATTAACCACAT+CGG | - | chr1.1:73380537-73380556 | MS.gene37667:intron | 25.0% |
| ! | TAATGGTATATAACATAGAC+TGG | + | chr1.1:73381718-73381737 | None:intergenic | 25.0% |
| ! | TGATAAAAAGATGATTCGTA+GGG | - | chr1.1:73380567-73380586 | MS.gene37667:intron | 25.0% |
| ! | TGCAAATTAAGACAGTTTAA+TGG | + | chr1.1:73381735-73381754 | None:intergenic | 25.0% |
| ! | TTCACGTTTATCAGATTTAA+TGG | - | chr1.1:73381024-73381043 | MS.gene37667:intron | 25.0% |
| !! | ATTGTTGTTGAATATATAGG+TGG | - | chr1.1:73381275-73381294 | MS.gene37667:intron | 25.0% |
| !!! | TCATAACATCTTTGCTTTTA+GGG | - | chr1.1:73380764-73380783 | MS.gene37667:intron | 25.0% |
| !!! | TTCATAACATCTTTGCTTTT+AGG | - | chr1.1:73380763-73380782 | MS.gene37667:intron | 25.0% |
| AATTACATTCAGATCAACCA+AGG | + | chr1.1:73381568-73381587 | None:intergenic | 30.0% | |
| CTCTATATCTTTAAGCAACA+AGG | + | chr1.1:73381764-73381783 | None:intergenic | 30.0% | |
| GAGAGATAAGATCAAAGAAA+TGG | - | chr1.1:73380871-73380890 | MS.gene37667:intron | 30.0% | |
| GTAGTTGCATGTAAATCTAA+TGG | + | chr1.1:73381654-73381673 | None:intergenic | 30.0% | |
| GTGGAAATTGTTATATGTAG+TGG | - | chr1.1:73380986-73381005 | MS.gene37667:intron | 30.0% | |
| TAGAGAACAAATGGATAGAA+TGG | - | chr1.1:73381779-73381798 | MS.gene37667:CDS | 30.0% | |
| ! | AAGAAATGGTTAGTTCAAGT+AGG | - | chr1.1:73380885-73380904 | MS.gene37667:intron | 30.0% |
| ! | AGAAATGGTTAGTTCAAGTA+GGG | - | chr1.1:73380886-73380905 | MS.gene37667:intron | 30.0% |
| ! | ATCACGCAATTTTATTGTCA+AGG | - | chr1.1:73381343-73381362 | MS.gene37667:intron | 30.0% |
| ! | GAAATATCTTGCCATTTACT+TGG | + | chr1.1:73381096-73381115 | None:intergenic | 30.0% |
| !! | TTTATGCTGATTCAGATCTT+CGG | + | chr1.1:73380524-73380543 | None:intergenic | 30.0% |
| AACATTGGAAGTCAGTTTAG+TGG | + | chr1.1:73381957-73381976 | None:intergenic | 35.0% | |
| AAGATTGATCTAATTCCAGG+AGG | - | chr1.1:73380962-73380981 | MS.gene37667:intron | 35.0% | |
| ACTCAGATACGGTTGTAAAA+TGG | + | chr1.1:73381891-73381910 | None:intergenic | 35.0% | |
| AGATTGATCTAATTCCAGGA+GGG | - | chr1.1:73380963-73380982 | MS.gene37667:intron | 35.0% | |
| GTCAAGATTGATCTAATTCC+AGG | - | chr1.1:73380959-73380978 | MS.gene37667:intron | 35.0% | |
| TGTCTCAAGTTTGACAACAT+TGG | + | chr1.1:73381972-73381991 | None:intergenic | 35.0% | |
| TGTGTTACCAACCAAGTAAA+TGG | - | chr1.1:73381082-73381101 | MS.gene37667:intron | 35.0% | |
| TGTTATATGTAGTGGTTTCG+AGG | - | chr1.1:73380994-73381013 | MS.gene37667:intron | 35.0% | |
| TTTATCATCATTGTCCGATG+TGG | + | chr1.1:73380554-73380573 | None:intergenic | 35.0% | |
| ! | GTATATAACATAGACTGGTC+AGG | + | chr1.1:73381713-73381732 | None:intergenic | 35.0% |
| ! | TATCTTGCCATTTACTTGGT+TGG | + | chr1.1:73381092-73381111 | None:intergenic | 35.0% |
| ! | TGAGGATTCTCAATTTTCCT+TGG | - | chr1.1:73381548-73381567 | MS.gene37667:CDS | 35.0% |
| !! | TCTAGGGTATTTCTGAGTTT+TGG | - | chr1.1:73381211-73381230 | MS.gene37667:intron | 35.0% |
| AAGACCTTGATGAGCTCAAA+AGG | + | chr1.1:73380655-73380674 | None:intergenic | 40.0% | |
| AGACCTTGATGAGCTCAAAA+GGG | + | chr1.1:73380654-73380673 | None:intergenic | 40.0% | |
| AGTATCAGATCACATAGGTG+AGG | - | chr1.1:73380796-73380815 | MS.gene37667:intron | 40.0% | |
| AGTTCAAGTAGGGTTGAAAC+TGG | - | chr1.1:73380896-73380915 | MS.gene37667:intron | 40.0% | |
| CGTTCAGTATCAGATCACAT+AGG | - | chr1.1:73380791-73380810 | MS.gene37667:intron | 40.0% | |
| CTACTAACTCATCCACAACT+TGG | - | chr1.1:73381687-73381706 | MS.gene37667:CDS | 40.0% | |
| GATTGATCTAATTCCAGGAG+GGG | - | chr1.1:73380964-73380983 | MS.gene37667:intron | 40.0% | |
| GTAAATGTGTGACTGTGATG+AGG | - | chr1.1:73381530-73381549 | MS.gene37667:intron | 40.0% | |
| GTAGGGAAGTTGAAAAGCAA+AGG | - | chr1.1:73380584-73380603 | MS.gene37667:intron | 40.0% | |
| TACCTTCGTTCAGCAGATTA+AGG | + | chr1.1:73381627-73381646 | None:intergenic | 40.0% | |
| TACGATCTAGCTAGAGTCTA+GGG | - | chr1.1:73381195-73381214 | MS.gene37667:intron | 40.0% | |
| TGCTCAGCTGCAAAATAAAC+TGG | - | chr1.1:73381320-73381339 | MS.gene37667:intron | 40.0% | |
| TTACGATCTAGCTAGAGTCT+AGG | - | chr1.1:73381194-73381213 | MS.gene37667:intron | 40.0% | |
| TTTGCAGCTGAGCAAGATTA+AGG | + | chr1.1:73381314-73381333 | None:intergenic | 40.0% | |
| !! | AATTAACTTGTACCTGTGCG+TGG | - | chr1.1:73381485-73381504 | MS.gene37667:intron | 40.0% |
| !!! | ATTTTTTTGTCTCCACGCAC+AGG | + | chr1.1:73381500-73381519 | None:intergenic | 40.0% |
| AAAAGGGAGTGAAGAACGGA+GGG | + | chr1.1:73380638-73380657 | None:intergenic | 45.0% | |
| ATAACAATTTCCACCCCTCC+TGG | + | chr1.1:73380980-73380999 | None:intergenic | 45.0% | |
| GCTCAAAAGGGAGTGAAGAA+CGG | + | chr1.1:73380642-73380661 | None:intergenic | 45.0% | |
| GGGAAGTTGAAAAGCAAAGG+AGG | - | chr1.1:73380587-73380606 | MS.gene37667:intron | 45.0% | |
| TGCCTTAATCTGCTGAACGA+AGG | - | chr1.1:73381622-73381641 | MS.gene37667:CDS | 45.0% | |
| ! | ACTCCCTTTTGAGCTCATCA+AGG | - | chr1.1:73380648-73380667 | MS.gene37667:intron | 45.0% |
| ! | GTGGCATTCACGTTAGTTTG+TGG | - | chr1.1:73382009-73382028 | MS.gene37667:intron | 45.0% |
| !!! | AATTTTGTAATTTTTTTTTA+GGG | - | chr1.1:73382130-73382149 | MS.gene37667:CDS | 5.0% |
| CAAAAGGGAGTGAAGAACGG+AGG | + | chr1.1:73380639-73380658 | None:intergenic | 50.0% | |
| TGATCTAATTCCAGGAGGGG+TGG | - | chr1.1:73380967-73380986 | MS.gene37667:intron | 50.0% | |
| ! | AGACTGGTCAGGCCAAGTTG+TGG | + | chr1.1:73381702-73381721 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 73380517 | 73382162 | 73380517 | ID=MS.gene37667 |
| chr1.1 | mRNA | 73380517 | 73382162 | 73380517 | ID=MS.gene37667.t1;Parent=MS.gene37667 |
| chr1.1 | exon | 73382010 | 73382162 | 73382010 | ID=MS.gene37667.t1.exon1;Parent=MS.gene37667.t1 |
| chr1.1 | CDS | 73382010 | 73382162 | 73382010 | ID=cds.MS.gene37667.t1;Parent=MS.gene37667.t1 |
| chr1.1 | exon | 73381547 | 73381894 | 73381547 | ID=MS.gene37667.t1.exon2;Parent=MS.gene37667.t1 |
| chr1.1 | CDS | 73381547 | 73381894 | 73381547 | ID=cds.MS.gene37667.t1;Parent=MS.gene37667.t1 |
| chr1.1 | exon | 73380517 | 73380528 | 73380517 | ID=MS.gene37667.t1.exon3;Parent=MS.gene37667.t1 |
| chr1.1 | CDS | 73380517 | 73380528 | 73380517 | ID=cds.MS.gene37667.t1;Parent=MS.gene37667.t1 |
| Gene Sequence |
| Protein sequence |
