AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006461

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006461.t1 MTR_1g103820 92.419 277 13 3 1 274 1 272 0.0 506
MS.gene006461.t1 MTR_8g045710 81.356 59 8 1 146 201 8 66 3.67e-25 98.6
MS.gene006461.t1 MTR_1g071390 41.525 118 17 2 100 217 1 66 1.48e-17 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006461.t1 AT4G14910 85.294 204 29 1 71 274 67 269 9.85e-125 357
MS.gene006461.t1 AT3G22425 86.364 198 27 0 80 277 73 270 1.07e-124 356
MS.gene006461.t1 AT4G14910 86.869 198 25 1 71 268 67 263 5.44e-123 352
MS.gene006461.t1 AT4G14910 86.869 198 25 1 71 268 67 263 5.50e-123 353
MS.gene006461.t1 AT3G22425 87.970 133 16 0 80 212 73 205 2.83e-83 251

Find 83 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATCAGTTAAAATTAATT+TGG 0.145809 1.2:-77325170 MS.gene006461:CDS
AAAATTAATTTGGATGGAAA+TGG 0.293645 1.2:-77325160 MS.gene006461:CDS
GAACATCTCCGCCGCTGCTT+TGG 0.321234 1.2:-77325367 MS.gene006461:CDS
GAAGATGTCGCTCTTGCTAT+TGG 0.325704 1.2:-77324371 MS.gene006461:CDS
AAATTAATTTGGATGGAAAT+GGG 0.326506 1.2:-77325159 MS.gene006461:CDS
GACCTTGTCCAGGATTTATC+TGG 0.333580 1.2:-77323530 MS.gene006461:intron
ATCATTTCAGGTGCTAGAAT+TGG 0.340324 1.2:-77325223 MS.gene006461:intron
CAGGCTTTGCTGCAGGCTCT+TGG 0.348810 1.2:-77324031 MS.gene006461:CDS
GGGGTTGCTGATAATAGTTC+TGG 0.350949 1.2:-77325139 MS.gene006461:CDS
GAAGTAAAACCTGAGTCAAT+TGG 0.354938 1.2:+77325312 None:intergenic
AACTGATACATTAGTTTCCT+TGG 0.365756 1.2:+77325183 None:intergenic
CATCTCCGCCGCTGCTTTGG+TGG 0.367364 1.2:-77325364 MS.gene006461:CDS
CAGTCATTGGTGAACACATC+TGG 0.380574 1.2:-77323217 MS.gene006461:CDS
AATATGATGGGAATTTGTTC+CGG 0.398429 1.2:+77322938 None:intergenic
TTCCGTCTCTGAAATCATCT+AGG 0.399239 1.2:-77325485 MS.gene006461:CDS
ACATACCTACTCAGAGGGTT+GGG 0.407867 1.2:-77323481 MS.gene006461:CDS
AGCCCTAGCAAATGCTTTGA+AGG 0.411264 1.2:+77322905 None:intergenic
AAAAGTGGGCATGTTGGGTT+GGG 0.421518 1.2:+77325438 None:intergenic
TCATCTCCAGGCTTTGCTGC+AGG 0.425450 1.2:-77324038 MS.gene006461:intron
TCAGTTAAAATTAATTTGGA+TGG 0.425680 1.2:-77325166 MS.gene006461:CDS
TGCTGCAGGCTCTTGGTGAT+AGG 0.428704 1.2:-77324024 MS.gene006461:CDS
ATCAAGCATATGATCAAGAA+AGG 0.443469 1.2:+77325111 None:intergenic
TCCGCCGCTGCTTTGGTGGA+TGG 0.445471 1.2:-77325360 MS.gene006461:CDS
GATGATTTCAGAGACGGAAA+TGG 0.447710 1.2:+77325489 None:intergenic
ATTCAGTGAGGTGAGATTGC+GGG 0.447732 1.2:+77325398 None:intergenic
AGCATTAGTTCACGTTTCTC+TGG 0.449545 1.2:-77323957 MS.gene006461:intron
TTTGAGTGCAACTGAGTTTG+TGG 0.451500 1.2:+77325519 None:intergenic
AAGTAAAACCTGAGTCAATT+GGG 0.453277 1.2:+77325313 None:intergenic
GTGATAGGAAGGGTATTAAC+CGG 0.453455 1.2:-77324009 MS.gene006461:CDS
AGGAAGGGTATTAACCGGTT+TGG 0.454615 1.2:-77324004 MS.gene006461:CDS
AGCAGCGGCGGAGATGTTCC+TGG 0.459063 1.2:+77325371 None:intergenic
CTAGAATTGGAGAGGTGAAA+AGG 0.463775 1.2:-77325210 MS.gene006461:CDS
CAACCTTCAAAGCATTTGCT+AGG 0.466744 1.2:-77322908 MS.gene006461:CDS
TTATCTGGCCGACCGCATCT+AGG 0.476067 1.2:-77323515 MS.gene006461:CDS
AATTAATTTGGATGGAAATG+GGG 0.492287 1.2:-77325158 MS.gene006461:CDS
TCCGTCTCTGAAATCATCTA+GGG 0.497267 1.2:-77325484 MS.gene006461:CDS
AACATACCTACTCAGAGGGT+TGG 0.513836 1.2:-77323482 MS.gene006461:CDS
AAATTCCCATCATATTATTG+AGG 0.526922 1.2:-77322931 MS.gene006461:CDS
GACGTACATGTAAAGGCTAC+AGG 0.527407 1.2:-77324425 MS.gene006461:CDS
TCAACTTTCCCAATTGACTC+AGG 0.527990 1.2:-77325321 MS.gene006461:intron
GCAGGCTCTTGGTGATAGGA+AGG 0.531782 1.2:-77324020 MS.gene006461:CDS
AGGTTGCCTCAATAATATGA+TGG 0.535692 1.2:+77322925 None:intergenic
GCTGCTTTGGTGGATGGCAA+TGG 0.538954 1.2:-77325354 MS.gene006461:CDS
CGACATCTTCATTTGTGTGA+TGG 0.542341 1.2:+77324384 None:intergenic
TTTCAGCAACTTGCTTCACA+TGG 0.550479 1.2:-77324455 MS.gene006461:intron
TGAACTAATGCTTCATCAAG+TGG 0.551680 1.2:+77323968 None:intergenic
GCCATCCACCAAAGCAGCGG+CGG 0.554726 1.2:+77325359 None:intergenic
GATTCAGTGAGGTGAGATTG+CGG 0.555231 1.2:+77325397 None:intergenic
GAGCAGAGAAGTTACCAAAC+CGG 0.559283 1.2:+77323990 None:intergenic
ACTGTTTGACGTACATGTAA+AGG 0.559891 1.2:-77324432 MS.gene006461:CDS
ACCCTAGATGATTTCAGAGA+CGG 0.560120 1.2:+77325483 None:intergenic
AACCTTCAAAGCATTTGCTA+GGG 0.561828 1.2:-77322907 MS.gene006461:CDS
CAGATGTGTTCACCAATGAC+TGG 0.561881 1.2:+77323218 None:intergenic
TCAAGCATATGATCAAGAAA+GGG 0.563364 1.2:+77325112 None:intergenic
GGTTGCCTCAATAATATGAT+GGG 0.565515 1.2:+77322926 None:intergenic
TAGAATTGGAGAGGTGAAAA+GGG 0.571453 1.2:-77325209 MS.gene006461:CDS
GCAGCGGCGGAGATGTTCCT+GGG 0.571795 1.2:+77325372 None:intergenic
TCGGTACCTGTCGAATGTGA+AGG 0.587275 1.2:+77323188 None:intergenic
TAAACCTTGGTATTGTTCCA+CGG 0.594113 1.2:+77322850 None:intergenic
CATACCTACTCAGAGGGTTG+GGG 0.594176 1.2:-77323480 MS.gene006461:CDS
CGGTACCTGTCGAATGTGAA+GGG 0.595893 1.2:+77323189 None:intergenic
ATTGCCATCCACCAAAGCAG+CGG 0.599331 1.2:+77325356 None:intergenic
TCACCTCACTGAATCCTCCC+AGG 0.604648 1.2:-77325389 MS.gene006461:CDS
TATGACCCTTCACATTCGAC+AGG 0.612406 1.2:-77323194 MS.gene006461:intron
TTCAGGTGCTAGAATTGGAG+AGG 0.615450 1.2:-77325218 MS.gene006461:intron
CGAGGGATGGAGGAGTGTTG+CGG 0.616676 1.2:+77322596 None:intergenic
GTTACTGTTCGAACGAGGGA+TGG 0.621361 1.2:+77322583 None:intergenic
GGTTGGGGCATACGACACTC+AGG 0.623135 1.2:-77323465 MS.gene006461:intron
TATGCCCCAACCCTCTGAGT+AGG 0.625898 1.2:+77323476 None:intergenic
CAGGCTCTTGGTGATAGGAA+GGG 0.631297 1.2:-77324019 MS.gene006461:CDS
ACTGTTCGAACGAGGGATGG+AGG 0.638947 1.2:+77322586 None:intergenic
CTTAAACATACCTACTCAGA+GGG 0.645466 1.2:-77323486 MS.gene006461:CDS
TGTCGCTCTTGCTATTGGAA+CGG 0.647644 1.2:-77324366 MS.gene006461:intron
GGGTGTTACTGTTCGAACGA+GGG 0.657513 1.2:+77322579 None:intergenic
AGGGTGTTACTGTTCGAACG+AGG 0.662956 1.2:+77322578 None:intergenic
AGTCATAACCTAGATGCGGT+CGG 0.664165 1.2:+77323507 None:intergenic
GAGTATGATACACGTCGCCG+TGG 0.666793 1.2:-77322867 MS.gene006461:CDS
ACTTAAACATACCTACTCAG+AGG 0.687734 1.2:-77323487 MS.gene006461:CDS
TTTAAGTCATAACCTAGATG+CGG 0.688778 1.2:+77323503 None:intergenic
AGAGGTGAAAAGGGTCACCA+AGG 0.711904 1.2:-77325200 MS.gene006461:CDS
GCGGCGGAGATGTTCCTGGG+AGG 0.718744 1.2:+77325375 None:intergenic
GTCGCCGTGGAACAATACCA+AGG 0.723313 1.2:-77322854 MS.gene006461:intron
GTTCCTGGGAGGATTCAGTG+AGG 0.795914 1.2:+77325386 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATGTTAAATCTATAAA+TGG - chr1.2:77324278-77324297 MS.gene006461:intron 10.0%
!! AATATATATTAAAAAAAGCA+TGG + chr1.2:77325125-77325144 None:intergenic 10.0%
!! TACTAAAAAAAACAAAAAAT+TGG + chr1.2:77325306-77325325 None:intergenic 10.0%
!!! TTAAATTTAAAATTGTTCAA+TGG - chr1.2:77323599-77323618 MS.gene006461:intron 10.0%
!!! TTTTTAGTAATTTTTTTTAG+GGG - chr1.2:77325317-77325336 MS.gene006461:intron 10.0%
!! AAAAATCGAAAATCTTAAAA+GGG + chr1.2:77322849-77322868 None:intergenic 15.0%
!! AAAATAAACTTATATCAAGA+TGG + chr1.2:77324523-77324542 None:intergenic 15.0%
!! TGTATCAGTTAAAATTAATT+TGG - chr1.2:77322948-77322967 MS.gene006461:intron 15.0%
!!! TACTTTTTTTTTATCATTTC+AGG - chr1.2:77322883-77322902 MS.gene006461:CDS 15.0%
!! AAAATCGAAAATCTTAAAAG+GGG + chr1.2:77322848-77322867 None:intergenic 20.0%
!! ATCTAAAAATAAATGTTGCA+AGG - chr1.2:77323323-77323342 MS.gene006461:intron 20.0%
!! ATGTCTGTTTAAATACTTTA+TGG - chr1.2:77324682-77324701 MS.gene006461:intron 20.0%
!! ATTTAACATATTTGTAAGCA+AGG + chr1.2:77324271-77324290 None:intergenic 20.0%
!! CAAAATGCAATTTAACTAAT+TGG - chr1.2:77323936-77323955 MS.gene006461:intron 20.0%
!! CCAATAAGAATTTAAGAAAA+TGG - chr1.2:77323112-77323131 MS.gene006461:intron 20.0%
!! GAAAAATCGAAAATCTTAAA+AGG + chr1.2:77322850-77322869 None:intergenic 20.0%
!! TGACATATATACATAGTTTA+GGG + chr1.2:77325359-77325378 None:intergenic 20.0%
!! TGTAGTAGATATTAGATAAA+GGG - chr1.2:77323408-77323427 MS.gene006461:intron 20.0%
!!! AAAATTAATTTGGATGGAAA+TGG - chr1.2:77322958-77322977 MS.gene006461:intron 20.0%
!!! AAATTAATTTGGATGGAAAT+GGG - chr1.2:77322959-77322978 MS.gene006461:intron 20.0%
!!! CCATTTTCTTAAATTCTTAT+TGG + chr1.2:77323115-77323134 None:intergenic 20.0%
!!! GTTTCTATTTTTTTAACGAA+AGG + chr1.2:77325442-77325461 None:intergenic 20.0%
!!! TCAGTTAAAATTAATTTGGA+TGG - chr1.2:77322952-77322971 MS.gene006461:intron 20.0%
! AAATCTGAATTGTTAGAATC+AGG - chr1.2:77323495-77323514 MS.gene006461:CDS 25.0%
! AAATTCCCATCATATTATTG+AGG - chr1.2:77325187-77325206 MS.gene006461:CDS 25.0%
! AATGTCAATTGAGAAAATAG+AGG + chr1.2:77325391-77325410 None:intergenic 25.0%
! AGGTTTCTGAATTGAAAATT+AGG - chr1.2:77324485-77324504 MS.gene006461:intron 25.0%
! AGTACATAAAAATAAGGAAC+GGG + chr1.2:77324833-77324852 None:intergenic 25.0%
! ATGTCAATTGAGAAAATAGA+GGG + chr1.2:77325390-77325409 None:intergenic 25.0%
! CACAAAACAGAAATTATAGT+GGG + chr1.2:77324546-77324565 None:intergenic 25.0%
! CAGTACATAAAAATAAGGAA+CGG + chr1.2:77324834-77324853 None:intergenic 25.0%
! CTACTTATTAAAGTTGTTTG+TGG - chr1.2:77323817-77323836 MS.gene006461:intron 25.0%
! CTCATCAGTACATAAAAATA+AGG + chr1.2:77324839-77324858 None:intergenic 25.0%
! GACATATATACATAGTTTAG+GGG + chr1.2:77325358-77325377 None:intergenic 25.0%
! GATTATAATCTTATGACTGT+GGG - chr1.2:77323975-77323994 MS.gene006461:CDS 25.0%
! GTCTATAAAAGATTCTATCT+AGG - chr1.2:77323461-77323480 MS.gene006461:intron 25.0%
! GTGACATATATACATAGTTT+AGG + chr1.2:77325360-77325379 None:intergenic 25.0%
! GTGTAGTAGATATTAGATAA+AGG - chr1.2:77323407-77323426 MS.gene006461:intron 25.0%
! TATAATCATTGTTAGAGTTG+TGG + chr1.2:77323962-77323981 None:intergenic 25.0%
! TGATTATAATCTTATGACTG+TGG - chr1.2:77323974-77323993 MS.gene006461:CDS 25.0%
! TGCACAAATGAATTCAAAAT+TGG + chr1.2:77325153-77325172 None:intergenic 25.0%
! TTAACTCAAATCTTTAACAG+AGG + chr1.2:77324220-77324239 None:intergenic 25.0%
!! AACTTGAAAACTACTTGTTA+AGG - chr1.2:77323561-77323580 MS.gene006461:intron 25.0%
!! AATTAATTTGGATGGAAATG+GGG - chr1.2:77322960-77322979 MS.gene006461:intron 25.0%
!!! AAGTAGTTTTCAAGTTAGAA+AGG + chr1.2:77323557-77323576 None:intergenic 25.0%
!!! CTATTTTTTTAACGAAAGGT+AGG + chr1.2:77325438-77325457 None:intergenic 25.0%
!!! GAAACTTTGAAACAATGTAA+TGG - chr1.2:77324306-77324325 MS.gene006461:intron 25.0%
AAGATTGAAGCTTGAAAAAG+TGG + chr1.2:77322698-77322717 None:intergenic 30.0%
AAGTAAAACCTGAGTCAATT+GGG + chr1.2:77322808-77322827 None:intergenic 30.0%
AGACGCAAAATTTATTGATG+TGG + chr1.2:77324378-77324397 None:intergenic 30.0%
AGATTGAAGCTTGAAAAAGT+GGG + chr1.2:77322697-77322716 None:intergenic 30.0%
ATATATACATAGTTTAGGGG+AGG + chr1.2:77325355-77325374 None:intergenic 30.0%
ATCAAGCATATGATCAAGAA+AGG + chr1.2:77323010-77323029 None:intergenic 30.0%
ATGATTCAACCATAGTATGA+AGG + chr1.2:77325486-77325505 None:intergenic 30.0%
CAATGAAATAGTACACATTC+TGG + chr1.2:77323349-77323368 None:intergenic 30.0%
GCACAAAACAGAAATTATAG+TGG + chr1.2:77324547-77324566 None:intergenic 30.0%
TAAAAATAAGGAACGGGAAA+AGG + chr1.2:77324827-77324846 None:intergenic 30.0%
TAGATATTAGATAAAGGGCT+AGG - chr1.2:77323413-77323432 MS.gene006461:intron 30.0%
TATAGTTGAATACTGAGCAT+GGG - chr1.2:77323380-77323399 MS.gene006461:intron 30.0%
TCAAGCATATGATCAAGAAA+GGG + chr1.2:77323009-77323028 None:intergenic 30.0%
TCAGAAAGTTCAGTTATCAA+TGG - chr1.2:77324397-77324416 MS.gene006461:CDS 30.0%
TCAGTTTATGTGAACAAACT+TGG - chr1.2:77323632-77323651 MS.gene006461:intron 30.0%
TGAGGATATTGCTTAAAGAT+TGG - chr1.2:77323869-77323888 MS.gene006461:intron 30.0%
TGTCAATTGAGAAAATAGAG+GGG + chr1.2:77325389-77325408 None:intergenic 30.0%
TTATAGTTGAATACTGAGCA+TGG - chr1.2:77323379-77323398 MS.gene006461:intron 30.0%
TTTAAGTCATAACCTAGATG+CGG + chr1.2:77324618-77324637 None:intergenic 30.0%
TTTGACTATTGAGCTTCAAA+TGG - chr1.2:77324746-77324765 MS.gene006461:intron 30.0%
TTTGTTCACATAAACTGACA+CGG + chr1.2:77323630-77323649 None:intergenic 30.0%
! AAATTGCATTTTGCACAGAA+AGG + chr1.2:77323929-77323948 None:intergenic 30.0%
! AACTGATACATTAGTTTCCT+TGG + chr1.2:77322938-77322957 None:intergenic 30.0%
! AATATGATGGGAATTTGTTC+CGG + chr1.2:77325183-77325202 None:intergenic 30.0%
! AATGCTAAAGATGTTTTACC+AGG - chr1.2:77325039-77325058 MS.gene006461:intron 30.0%
! AGAATCAGGTTGTAATGATA+AGG - chr1.2:77323509-77323528 MS.gene006461:CDS 30.0%
! TAAATTCAAGCCTCAGTTTT+CGG - chr1.2:77325096-77325115 MS.gene006461:intron 30.0%
ACGCCTTCAATATAAAGTGT+TGG - chr1.2:77323210-77323229 MS.gene006461:CDS 35.0%
ACTGTTTGACGTACATGTAA+AGG - chr1.2:77323686-77323705 MS.gene006461:intron 35.0%
ACTTAAACATACCTACTCAG+AGG - chr1.2:77324631-77324650 MS.gene006461:intron 35.0%
AGGTTGCCTCAATAATATGA+TGG + chr1.2:77325196-77325215 None:intergenic 35.0%
ATTTACGTTACTTCATCTCC+AGG - chr1.2:77324068-77324087 MS.gene006461:intron 35.0%
CGAACATGCTTCATTATCTT+AGG - chr1.2:77323845-77323864 MS.gene006461:intron 35.0%
CTGACCTAGATTCTATGTAT+CGG + chr1.2:77324952-77324971 None:intergenic 35.0%
CTTAAACATACCTACTCAGA+GGG - chr1.2:77324632-77324651 MS.gene006461:intron 35.0%
GAAGTAAAACCTGAGTCAAT+TGG + chr1.2:77322809-77322828 None:intergenic 35.0%
GCACCAACACTTTATATTGA+AGG + chr1.2:77323216-77323235 None:intergenic 35.0%
GGTTGCCTCAATAATATGAT+GGG + chr1.2:77325195-77325214 None:intergenic 35.0%
GTCAATTGAGAAAATAGAGG+GGG + chr1.2:77325388-77325407 None:intergenic 35.0%
GTTTGAGAGATACTAAACCT+TGG + chr1.2:77325284-77325303 None:intergenic 35.0%
TAAACCTTGGTATTGTTCCA+CGG + chr1.2:77325271-77325290 None:intergenic 35.0%
TAGAATTGGAGAGGTGAAAA+GGG - chr1.2:77322909-77322928 MS.gene006461:CDS 35.0%
TATGAAGGACAGAAGAAGAA+GGG + chr1.2:77325471-77325490 None:intergenic 35.0%
TCTAGCAGTACTTGTTCATA+AGG - chr1.2:77324465-77324484 MS.gene006461:intron 35.0%
TGAACTAATGCTTCATCAAG+TGG + chr1.2:77324153-77324172 None:intergenic 35.0%
TGCGTAATAAAAGACATACG+TGG + chr1.2:77323273-77323292 None:intergenic 35.0%
TTCTTCTGTCCTTCATACTA+TGG - chr1.2:77325474-77325493 MS.gene006461:CDS 35.0%
! AACCTTCAAAGCATTTGCTA+GGG - chr1.2:77325211-77325230 MS.gene006461:CDS 35.0%
! AGCTTTAGTACGATGATAGA+TGG + chr1.2:77324045-77324064 None:intergenic 35.0%
! ATCATTTCAGGTGCTAGAAT+TGG - chr1.2:77322895-77322914 MS.gene006461:CDS 35.0%
! TGCTTCATTATCTTAGGTTG+AGG - chr1.2:77323851-77323870 MS.gene006461:intron 35.0%
! TTATGTACTGATGAGTCTTC+TGG - chr1.2:77324844-77324863 MS.gene006461:intron 35.0%
! TTCATTTGTGCACAGTTTTC+CGG - chr1.2:77325161-77325180 MS.gene006461:CDS 35.0%
!! AATGAGAAAGCATTGACACA+AGG + chr1.2:77324442-77324461 None:intergenic 35.0%
AAATAAGGAACGGGAAAAGG+TGG + chr1.2:77324824-77324843 None:intergenic 40.0%
ACCCTAGATGATTTCAGAGA+CGG + chr1.2:77322638-77322657 None:intergenic 40.0%
AGCATTAGTTCACGTTTCTC+TGG - chr1.2:77324161-77324180 MS.gene006461:intron 40.0%
ATAAATCCTGGACAAGGTCA+GGG + chr1.2:77324587-77324606 None:intergenic 40.0%
CATAGAATCTAGGTCAGAGT+CGG - chr1.2:77324955-77324974 MS.gene006461:intron 40.0%
CGAAAATCTTAAAAGGGGCA+GGG + chr1.2:77322843-77322862 None:intergenic 40.0%
CGACATCTTCATTTGTGTGA+TGG + chr1.2:77323737-77323756 None:intergenic 40.0%
CTAGAATTGGAGAGGTGAAA+AGG - chr1.2:77322908-77322927 MS.gene006461:CDS 40.0%
CTTGAAAAAGTGGGCATGTT+GGG + chr1.2:77322688-77322707 None:intergenic 40.0%
GAAAATCTTAAAAGGGGCAG+GGG + chr1.2:77322842-77322861 None:intergenic 40.0%
GATGATTTCAGAGACGGAAA+TGG + chr1.2:77322632-77322651 None:intergenic 40.0%
GTATGAAGGACAGAAGAAGA+AGG + chr1.2:77325472-77325491 None:intergenic 40.0%
TATACAGCTCACTTAGCTGT+TGG + chr1.2:77323783-77323802 None:intergenic 40.0%
TCGAAAATCTTAAAAGGGGC+AGG + chr1.2:77322844-77322863 None:intergenic 40.0%
TGGCAGTGCAAAAGTAACAA+AGG + chr1.2:77323063-77323082 None:intergenic 40.0%
TTTGAGTGCAACTGAGTTTG+TGG + chr1.2:77322602-77322621 None:intergenic 40.0%
! ACATCCTTGTTTGTAGTTGG+TGG - chr1.2:77324867-77324886 MS.gene006461:intron 40.0%
! ATTTGAGTTAACGCGTGTTG+AGG - chr1.2:77324229-77324248 MS.gene006461:intron 40.0%
! CAACCTTCAAAGCATTTGCT+AGG - chr1.2:77325210-77325229 MS.gene006461:CDS 40.0%
! TCAACTTTCCCAATTGACTC+AGG - chr1.2:77322797-77322816 MS.gene006461:intron 40.0%
! TGGACATCCTTGTTTGTAGT+TGG - chr1.2:77324864-77324883 MS.gene006461:intron 40.0%
! TTATATTGAAGGCGTGTTCC+AGG + chr1.2:77323205-77323224 None:intergenic 40.0%
! TTTCAGCAACTTGCTTCACA+TGG - chr1.2:77323663-77323682 MS.gene006461:intron 40.0%
!! GGTACCGATACATAGAATCT+AGG - chr1.2:77324945-77324964 MS.gene006461:intron 40.0%
!! GTGATAGGAAGGGTATTAAC+CGG - chr1.2:77324109-77324128 MS.gene006461:intron 40.0%
!! TCCGTCTCTGAAATCATCTA+GGG - chr1.2:77322634-77322653 MS.gene006461:intron 40.0%
!! TTCCGTCTCTGAAATCATCT+AGG - chr1.2:77322633-77322652 MS.gene006461:intron 40.0%
!!! GGAGCATTTTTTCCAGTCAT+TGG - chr1.2:77324888-77324907 MS.gene006461:intron 40.0%
AAAAAGTGGGCATGTTGGGT+TGG + chr1.2:77322684-77322703 None:intergenic 45.0%
AAAAGTGGGCATGTTGGGTT+GGG + chr1.2:77322683-77322702 None:intergenic 45.0%
AACATACCTACTCAGAGGGT+TGG - chr1.2:77324636-77324655 MS.gene006461:intron 45.0%
ACATACCTACTCAGAGGGTT+GGG - chr1.2:77324637-77324656 MS.gene006461:intron 45.0%
AGCCCTAGCAAATGCTTTGA+AGG + chr1.2:77325216-77325235 None:intergenic 45.0%
AGTCATAACCTAGATGCGGT+CGG + chr1.2:77324614-77324633 None:intergenic 45.0%
ATAAAGGGCTAGGTGCACAT+GGG - chr1.2:77323423-77323442 MS.gene006461:intron 45.0%
ATTCAGTGAGGTGAGATTGC+GGG + chr1.2:77322723-77322742 None:intergenic 45.0%
CAGATGTGTTCACCAATGAC+TGG + chr1.2:77324903-77324922 None:intergenic 45.0%
CAGTCATTGGTGAACACATC+TGG - chr1.2:77324901-77324920 MS.gene006461:intron 45.0%
GACCTTGTCCAGGATTTATC+TGG - chr1.2:77324588-77324607 MS.gene006461:intron 45.0%
GACGTACATGTAAAGGCTAC+AGG - chr1.2:77323693-77323712 MS.gene006461:intron 45.0%
GAGCAGAGAAGTTACCAAAC+CGG + chr1.2:77324131-77324150 None:intergenic 45.0%
GATAAATCCTGGACAAGGTC+AGG + chr1.2:77324588-77324607 None:intergenic 45.0%
GATTCAGTGAGGTGAGATTG+CGG + chr1.2:77322724-77322743 None:intergenic 45.0%
GCTTGAAAAAGTGGGCATGT+TGG + chr1.2:77322689-77322708 None:intergenic 45.0%
GGGGTTGCTGATAATAGTTC+TGG - chr1.2:77322979-77322998 MS.gene006461:intron 45.0%
GTAGGCAACTCTTAACAGCA+GGG + chr1.2:77325420-77325439 None:intergenic 45.0%
TAGGCAACTCTTAACAGCAG+GGG + chr1.2:77325419-77325438 None:intergenic 45.0%
TATGACCCTTCACATTCGAC+AGG - chr1.2:77324924-77324943 MS.gene006461:intron 45.0%
TGCTCCACCAACTACAAACA+AGG + chr1.2:77324874-77324893 None:intergenic 45.0%
TTCAGGTGCTAGAATTGGAG+AGG - chr1.2:77322900-77322919 MS.gene006461:CDS 45.0%
! GAAGATGTCGCTCTTGCTAT+TGG - chr1.2:77323747-77323766 MS.gene006461:intron 45.0%
!! AGGAAGGGTATTAACCGGTT+TGG - chr1.2:77324114-77324133 MS.gene006461:intron 45.0%
!! TGTACAAAGAGCATCTAGCC+TGG + chr1.2:77325060-77325079 None:intergenic 45.0%
!! TGTCGCTCTTGCTATTGGAA+CGG - chr1.2:77323752-77323771 MS.gene006461:intron 45.0%
!!! TTTTTTAGTAATTTTTTTTA+GGG - chr1.2:77325316-77325335 MS.gene006461:intron 5.0%
!!! TTTTTTTAGTAATTTTTTTT+AGG - chr1.2:77325315-77325334 MS.gene006461:intron 5.0%
AAGGAACGGGAAAAGGTGGA+AGG + chr1.2:77324820-77324839 None:intergenic 50.0%
AGAGGTGAAAAGGGTCACCA+AGG - chr1.2:77322918-77322937 MS.gene006461:CDS 50.0%
AGCATGGTCTCCGAAAACTG+AGG + chr1.2:77325109-77325128 None:intergenic 50.0%
ATTGCCATCCACCAAAGCAG+CGG + chr1.2:77322765-77322784 None:intergenic 50.0%
CATACCTACTCAGAGGGTTG+GGG - chr1.2:77324638-77324657 MS.gene006461:intron 50.0%
CGGTACCTGTCGAATGTGAA+GGG + chr1.2:77324932-77324951 None:intergenic 50.0%
CTAGGTGCACATGGGATGAA+TGG - chr1.2:77323431-77323450 MS.gene006461:intron 50.0%
GATAAAGGGCTAGGTGCACA+TGG - chr1.2:77323422-77323441 MS.gene006461:intron 50.0%
GGCCAGATAAATCCTGGACA+AGG + chr1.2:77324593-77324612 None:intergenic 50.0%
GGTAGGCAACTCTTAACAGC+AGG + chr1.2:77325421-77325440 None:intergenic 50.0%
TCGGTACCTGTCGAATGTGA+AGG + chr1.2:77324933-77324952 None:intergenic 50.0%
! CAGGCTCTTGGTGATAGGAA+GGG - chr1.2:77324099-77324118 MS.gene006461:intron 50.0%
!! GCACATGTTGGTGTCAGACA+TGG + chr1.2:77323183-77323202 None:intergenic 50.0%
AATCCTGGACAAGGTCAGGG+AGG + chr1.2:77324584-77324603 None:intergenic 55.0%
ACTGTTCGAACGAGGGATGG+AGG + chr1.2:77325535-77325554 None:intergenic 55.0%
GAGTATGATACACGTCGCCG+TGG - chr1.2:77325251-77325270 MS.gene006461:intron 55.0%
GCAACTCTTAACAGCAGGGG+AGG + chr1.2:77325416-77325435 None:intergenic 55.0%
GGTGCACATGGGATGAATGG+TGG - chr1.2:77323434-77323453 MS.gene006461:intron 55.0%
GTCGCCGTGGAACAATACCA+AGG - chr1.2:77325264-77325283 MS.gene006461:intron 55.0%
GTCTGACACCAACATGTGCC+TGG - chr1.2:77323184-77323203 MS.gene006461:intron 55.0%
GTTCCTGGGAGGATTCAGTG+AGG + chr1.2:77322735-77322754 None:intergenic 55.0%
TATGCCCCAACCCTCTGAGT+AGG + chr1.2:77324645-77324664 None:intergenic 55.0%
TCACCTCACTGAATCCTCCC+AGG - chr1.2:77322729-77322748 MS.gene006461:intron 55.0%
TCATCTCCAGGCTTTGCTGC+AGG - chr1.2:77324080-77324099 MS.gene006461:intron 55.0%
TCTTAAAAGGGGCAGGGGTG+AGG + chr1.2:77322837-77322856 None:intergenic 55.0%
TTATCTGGCCGACCGCATCT+AGG - chr1.2:77324603-77324622 MS.gene006461:intron 55.0%
TTGCACTGCCACACGTACAC+CGG - chr1.2:77323072-77323091 MS.gene006461:intron 55.0%
TTTCCTCCCTGACCTTGTCC+AGG - chr1.2:77324578-77324597 MS.gene006461:intron 55.0%
! GCAGGCTCTTGGTGATAGGA+AGG - chr1.2:77324098-77324117 MS.gene006461:intron 55.0%
! GCTGCTTTGGTGGATGGCAA+TGG - chr1.2:77322764-77322783 MS.gene006461:intron 55.0%
! TGCTGCAGGCTCTTGGTGAT+AGG - chr1.2:77324094-77324113 MS.gene006461:intron 55.0%
CAAGAGCCTGCAGCAAAGCC+TGG + chr1.2:77324089-77324108 None:intergenic 60.0%
CAGGCTTTGCTGCAGGCTCT+TGG - chr1.2:77324087-77324106 MS.gene006461:intron 60.0%
GAACATCTCCGCCGCTGCTT+TGG - chr1.2:77322751-77322770 MS.gene006461:intron 60.0%
GCGGTCGGCCAGATAAATCC+TGG + chr1.2:77324599-77324618 None:intergenic 60.0%
GGCGACTACGTGTCAGTGTC+CGG + chr1.2:77323094-77323113 None:intergenic 60.0%
GGCGTGTTCCAGGCACATGT+TGG + chr1.2:77323195-77323214 None:intergenic 60.0%
GGTTGGGGCATACGACACTC+AGG - chr1.2:77324653-77324672 MS.gene006461:intron 60.0%
GTCAGTGTCCGGTGTACGTG+TGG + chr1.2:77323083-77323102 None:intergenic 60.0%
!! AGGAGTGTTGCGGCACTGTG+AGG + chr1.2:77325515-77325534 None:intergenic 60.0%
!! CGAGGGATGGAGGAGTGTTG+CGG + chr1.2:77325525-77325544 None:intergenic 60.0%
AGCAGCGGCGGAGATGTTCC+TGG + chr1.2:77322750-77322769 None:intergenic 65.0%
GCAGCGGCGGAGATGTTCCT+GGG + chr1.2:77322749-77322768 None:intergenic 65.0%
GCCATCCACCAAAGCAGCGG+CGG + chr1.2:77322762-77322781 None:intergenic 65.0%
! CATCTCCGCCGCTGCTTTGG+TGG - chr1.2:77322754-77322773 MS.gene006461:intron 65.0%
! TCCGCCGCTGCTTTGGTGGA+TGG - chr1.2:77322758-77322777 MS.gene006461:intron 65.0%
GCGGCGGAGATGTTCCTGGG+AGG + chr1.2:77322746-77322765 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 77322584 77325556 77322584 ID=MS.gene006461
chr1.2 mRNA 77322584 77325556 77322584 ID=MS.gene006461.t1;Parent=MS.gene006461
chr1.2 exon 77325322 77325556 77325322 ID=MS.gene006461.t1.exon1;Parent=MS.gene006461.t1
chr1.2 CDS 77325322 77325556 77325322 ID=cds.MS.gene006461.t1;Parent=MS.gene006461.t1
chr1.2 exon 77325111 77325235 77325111 ID=MS.gene006461.t1.exon2;Parent=MS.gene006461.t1
chr1.2 CDS 77325111 77325235 77325111 ID=cds.MS.gene006461.t1;Parent=MS.gene006461.t1
chr1.2 exon 77324367 77324471 77324367 ID=MS.gene006461.t1.exon3;Parent=MS.gene006461.t1
chr1.2 CDS 77324367 77324471 77324367 ID=cds.MS.gene006461.t1;Parent=MS.gene006461.t1
chr1.2 exon 77323958 77324050 77323958 ID=MS.gene006461.t1.exon4;Parent=MS.gene006461.t1
chr1.2 CDS 77323958 77324050 77323958 ID=cds.MS.gene006461.t1;Parent=MS.gene006461.t1
chr1.2 exon 77323466 77323540 77323466 ID=MS.gene006461.t1.exon5;Parent=MS.gene006461.t1
chr1.2 CDS 77323466 77323540 77323466 ID=cds.MS.gene006461.t1;Parent=MS.gene006461.t1
chr1.2 exon 77323195 77323257 77323195 ID=MS.gene006461.t1.exon6;Parent=MS.gene006461.t1
chr1.2 CDS 77323195 77323257 77323195 ID=cds.MS.gene006461.t1;Parent=MS.gene006461.t1
chr1.2 exon 77322855 77322964 77322855 ID=MS.gene006461.t1.exon7;Parent=MS.gene006461.t1
chr1.2 CDS 77322855 77322964 77322855 ID=cds.MS.gene006461.t1;Parent=MS.gene006461.t1
chr1.2 exon 77322584 77322620 77322584 ID=MS.gene006461.t1.exon8;Parent=MS.gene006461.t1
chr1.2 CDS 77322584 77322620 77322584 ID=cds.MS.gene006461.t1;Parent=MS.gene006461.t1
Gene Sequence

>MS.gene006461

ATGGAGCTGACTTTACCACAAACTCAGTTGCACTCAAAATATCTTCCATTTCCGTCTCTGAAATCATCTAGGGTTTCTGTTTTTCATAGAATTACACCCAACCCAACATGCCCACTTTTTCAAGCTTCAATCTTTTCCCGCAATCTCACCTCACTGAATCCTCCCAGGAACATCTCCGCCGCTGCTTTGGTGGATGGCAATGGCAACAACACTTCAACTTTCCCAATTGACTCAGGTTTTACTTCTTTTTCCTCACCCCTGCCCCTTTTAAGATTTTCGATTTTTCAATGAAATTTGATTACTTTTTTTTTATCATTTCAGGTGCTAGAATTGGAGAGGTGAAAAGGGTCACCAAGGAAACTAATGTATCAGTTAAAATTAATTTGGATGGAAATGGGGTTGCTGATAATAGTTCTGGAATTCCCTTTCTTGATCATATGCTTGATGTTAGCATCTTCAACTCAATTGCTTTTATGCCTTTGTTACTTTTGCACTGCCACACGTACACCGGACACTGACACGTAGTCGCCAATAAGAATTTAAGAAAATGGAAGTGATTGAACGTAATTATGTATGTAACTTGTAAGTGTCGTGTTCCATGTCTGACACCAACATGTGCCTGGAACACGCCTTCAATATAAAGTGTTGGTGCTAAATAGCTTTTTTCAATATGTTATTCTTGCTAACCACGTATGTCTTTTATTACGCAACATGAGATTTCAATCACTACACACACATAATCTAAAAATAAATGTTGCAAGGCCAGAATGTGTACTATTTCATTGTGAGTTTTTGTTATAGTTGAATACTGAGCATGGGAATCGTGTAGTAGATATTAGATAAAGGGCTAGGTGCACATGGGATGAATGGTGGACTTGTCTATAAAAGATTCTATCTAGGATGTTATTGAAAAATCTGAATTGTTAGAATCAGGTTGTAATGATAAGGCTTTTTCTTTTTACTTTTGTCTCCTTTCTAACTTGAAAACTACTTGTTAAGGATGTTATTATGTATTTTAAATTTAAAATTGTTCAATGGATACACCGTGTCAGTTTATGTGAACAAACTTGGTTGTGTTTTTTCAGCAACTTGCTTCACATGGACTGTTTGACGTACATGTAAAGGCTACAGGTGACACGCATATTGATGACCATCACACAAATGAAGATGTCGCTCTTGCTATTGGAACGGTACTGCCAACAGCTAAGTGAGCTGTATATTTATTTTTTTGCTCTACTTATTAAAGTTGTTTGTGGAAAGTCGAACATGCTTCATTATCTTAGGTTGAGGATATTGCTTAAAGATTGGTGTGAAGTTATTATATCTCTGTATTTGACTTTCTCCTTTCTGTGCAAAATGCAATTTAACTAATTGGCCACAACTCTAACAATGATTATAATCTTATGACTGTGGGAATTTTCAATTAGCTTAAAGCTTTTTTTTTTAGTATATTAGCATCCATCTATCATCGTACTAAAGCTTAGATTTACGTTACTTCATCTCCAGGCTTTGCTGCAGGCTCTTGGTGATAGGAAGGGTATTAACCGGTTTGGTAACTTCTCTGCTCCACTTGATGAAGCATTAGTTCACGTTTCTCTGGTAATTTGCGTACTGTTTGTAGCAATAATCATAACCTCTGTTAAAGATTTGAGTTAACGCGTGTTGAGGAATGCATATGATTTGTCCTTGCTTACAAATATGTTAAATCTATAAATGGATGAAGAAACTTTGAAACAATGTAATGGATTTTGCATGTGCTGCTTAACAATCATACATTATCATGTTGATTATCCACATCAATAAATTTTGCGTCTCAGAAAGTTCAGTTATCAATGGCGCACAAGTGAGCTTGTTGCCTTGTGTCAATGCTTTCTCATTGACTCTAGCAGTACTTGTTCATAAGGTTTCTGAATTGAAAATTAGGAAGTTTTAACGACCATCTTGATATAAGTTTATTTTCCCACTATAATTTCTGTTTTGTGCTTCATTATAGCTTTCCTCCCTGACCTTGTCCAGGATTTATCTGGCCGACCGCATCTAGGTTATGACTTAAACATACCTACTCAGAGGGTTGGGGCATACGACACTCAGGTAGTACATGTCTGTTTAAATACTTTATGGTTCAGTTTAGATTTTTTTTTTTTTTTTTTTTATATATAGCTTTTGACTATTGAGCTTCAAATGGATAGATCAGATATTTGAATGATGTGTATCATTTATCATATTACTTGTTCCTTCCACCTTTTCCCGTTCCTTATTTTTATGTACTGATGAGTCTTCTGGACATCCTTGTTTGTAGTTGGTGGAGCATTTTTTCCAGTCATTGGTGAACACATCTGGTATGACCCTTCACATTCGACAGGTACCGATACATAGAATCTAGGTCAGAGTCGGTTATAGTCATTGCATTTTTTATAAGTATATCTGTGCATTTCATTATGAAAGAAATATGCTGAATGCTAAAGATGTTTTACCAGGCTAGATGCTCTTTGTACATTCTTCATGCACTGATTAAATTCAAGCCTCAGTTTTCGGAGACCATGCTTTTTTTAATATATATTTTATTCCAATTTTGAATTCATTTGTGCACAGTTTTCCGGAACAAATTCCCATCATATTATTGAGGCAACCTTCAAAGCATTTGCTAGGGCTCTTCGACAAGCTACAGAGTATGATACACGTCGCCGTGGAACAATACCAAGGTTTAGTATCTCTCAAACCAATTTTTTGTTTTTTTTAGTAATTTTTTTTAGGGGAAAAAACACTCACCTCCCCTAAACTATGTATATATGTCACTTACACCCCCTCTATTTTCTCAATTGACATTAACCTCCCCTGCTGTTAAGAGTTGCCTACCTTTCGTTAAAAAAATAGAAACGTCAAACCCTTCTTCTTCTGTCCTTCATACTATGGTTGAATCATATGCAACCTCACAGTGCCGCAACACTCCTCCATCCCTCGTTCGAACAGTAA
Protein sequence

>MS.gene006461.t1

MELTLPQTQLHSKYLPFPSLKSSRVSVFHRITPNPTCPLFQASIFSRNLTSLNPPRNISAAALVDGNGNNTSTFPIDSGARIGEVKRVTKETNVSVKINLDGNGVADNSSGIPFLDHMLDQLASHGLFDVHVKATGDTHIDDHHTNEDVALAIGTALLQALGDRKGINRFGNFSAPLDEALVHVSLDLSGRPHLGYDLNIPTQRVGAYDTQLVEHFFQSLVNTSGMTLHIRQFSGTNSHHIIEATFKAFARALRQATEYDTRRRGTIPSAATLLHPSFEQ