Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007018.t1 | XP_013468487.1 | 99.4 | 164 | 1 | 0 | 1 | 164 | 1 | 164 | 3.10E-88 | 334.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007018.t1 | Q9FDZ9 | 81.1 | 164 | 31 | 0 | 1 | 164 | 1 | 164 | 4.7e-77 | 288.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007018.t1 | I3T8M7 | 99.4 | 164 | 1 | 0 | 1 | 164 | 1 | 164 | 2.2e-88 | 334.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene23031 | MS.gene007018 | PPI |
| MS.gene50640 | MS.gene007018 | PPI |
| MS.gene79286 | MS.gene007018 | PPI |
| MS.gene000360 | MS.gene007018 | PPI |
| MS.gene036076 | MS.gene007018 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007018.t1 | MTR_1g069905 | 99.390 | 164 | 1 | 0 | 1 | 164 | 1 | 164 | 4.88e-120 | 335 |
| MS.gene007018.t1 | MTR_7g107420 | 98.780 | 164 | 2 | 0 | 1 | 164 | 1 | 164 | 9.03e-120 | 335 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007018.t1 | AT1G57860 | 81.098 | 164 | 31 | 0 | 1 | 164 | 1 | 164 | 2.50e-101 | 288 |
| MS.gene007018.t1 | AT1G57660 | 81.098 | 164 | 31 | 0 | 1 | 164 | 1 | 164 | 2.50e-101 | 288 |
| MS.gene007018.t1 | AT1G09690 | 81.098 | 164 | 31 | 0 | 1 | 164 | 1 | 164 | 4.47e-101 | 288 |
| MS.gene007018.t1 | AT1G09590 | 81.098 | 164 | 31 | 0 | 1 | 164 | 1 | 164 | 4.47e-101 | 288 |
Find 0 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTGATTTATTGTATTTTA+GGG | + | 51722:45041-45060 | MS.gene007018:intron | 10.0% |
| !!! | TTATTGATTTATTGTATTTT+AGG | + | 51722:45040-45059 | MS.gene007018:intron | 10.0% |
| !! | ATATAATTACATTGATCAAA+TGG | - | 51722:45394-45413 | None:intergenic | 15.0% |
| !! | TATAATTACATTGATCAAAT+GGG | - | 51722:45393-45412 | None:intergenic | 15.0% |
| !!! | ATTATATGTTGTTATTTATC+TGG | + | 51722:45407-45426 | MS.gene007018:intron | 15.0% |
| !!! | GAATTTTGTAAATTTACTTG+TGG | + | 51722:45233-45252 | MS.gene007018:intron | 20.0% |
| !!! | TATTGAAATATTGCTATTTG+TGG | + | 51722:45490-45509 | MS.gene007018:intron | 20.0% |
| ! | AAAACCACAGAACTAAAATT+CGG | - | 51722:44960-44979 | None:intergenic | 25.0% |
| ! | GATTAAGAATGATCAACTTA+AGG | + | 51722:45607-45626 | MS.gene007018:CDS | 25.0% |
| !! | TGTGTGCTTTAATTACATTT+TGG | + | 51722:45086-45105 | MS.gene007018:intron | 25.0% |
| AGATCATTAACCACATCATA+TGG | - | 51722:45740-45759 | None:intergenic | 30.0% | |
| CATTCTTAATCTTCCTCAAA+CGG | - | 51722:45598-45617 | None:intergenic | 30.0% | |
| GATGTGGTTAATGATCTTAA+GGG | + | 51722:45743-45762 | MS.gene007018:CDS | 30.0% | |
| TGATGTGGTTAATGATCTTA+AGG | + | 51722:45742-45761 | MS.gene007018:CDS | 30.0% | |
| TTAACCACATCATATGGAAT+TGG | - | 51722:45734-45753 | None:intergenic | 30.0% | |
| ! | AGGGTTTATGATTTTCTATG+CGG | + | 51722:45060-45079 | MS.gene007018:intron | 30.0% |
| !! | AATATTGCTATTTGTGGTTC+AGG | + | 51722:45496-45515 | MS.gene007018:intron | 30.0% |
| !!! | ATTTCCGAATTTTAGTTCTG+TGG | + | 51722:44953-44972 | MS.gene007018:intron | 30.0% |
| TACTCCAATTCCATATGATG+TGG | + | 51722:45727-45746 | MS.gene007018:CDS | 35.0% | |
| TATGTCGATATCAGGGTTAA+CGG | + | 51722:44809-44828 | MS.gene007018:CDS | 35.0% | |
| TGTTTGCAAGAGAGTTTATC+AGG | + | 51722:45442-45461 | MS.gene007018:intron | 35.0% | |
| AAACCTGGTTTCATGGTTGA+AGG | + | 51722:45686-45705 | MS.gene007018:CDS | 40.0% | |
| AACGTTACCAAACGTGCTAT+TGG | + | 51722:44890-44909 | MS.gene007018:CDS | 40.0% | |
| AGGTTAGGAACAAGATTCTC+AGG | + | 51722:45516-45535 | MS.gene007018:intron | 40.0% | |
| ATTGTACCCTTCTTCCTGAA+TGG | - | 51722:44749-44768 | None:intergenic | 40.0% | |
| GTGGTTAATGATCTTAAGGG+AGG | + | 51722:45746-45765 | MS.gene007018:CDS | 40.0% | |
| TACCTCAGAACCTATCACAT+CGG | + | 51722:44782-44801 | MS.gene007018:CDS | 40.0% | |
| TCAACCATGAAACCAGGTTT+AGG | - | 51722:45686-45705 | None:intergenic | 40.0% | |
| TCAACTTAAGGCTGATGCTA+AGG | + | 51722:45619-45638 | MS.gene007018:CDS | 40.0% | |
| ! | TGCTATTTGTGGTTCAGGTT+AGG | + | 51722:45501-45520 | MS.gene007018:intron | 40.0% |
| AAACCAGGTTTAGGTCCTTC+TGG | - | 51722:45677-45696 | None:intergenic | 45.0% | |
| AAGGCTGATGCTAAGGCAAA+GGG | + | 51722:45626-45645 | MS.gene007018:CDS | 45.0% | |
| ACTCTTTCTCTCGTCCATTC+AGG | + | 51722:44732-44751 | MS.gene007018:CDS | 45.0% | |
| AGGACCTAAACCTGGTTTCA+TGG | + | 51722:45679-45698 | MS.gene007018:CDS | 45.0% | |
| ATGCCACACAAGTTCTACCA+TGG | + | 51722:44848-44867 | MS.gene007018:CDS | 45.0% | |
| CATGGTTGAAGGAGCTACAT+TGG | + | 51722:45697-45716 | MS.gene007018:CDS | 45.0% | |
| CGGCGATTATGTCGATATCA+GGG | + | 51722:44802-44821 | MS.gene007018:CDS | 45.0% | |
| CTCAACACCAATAGCACGTT+TGG | - | 51722:44900-44919 | None:intergenic | 45.0% | |
| GAAGAGAATCCATGTTCGAG+TGG | + | 51722:45538-45557 | MS.gene007018:CDS | 45.0% | |
| GACATAATCGCCGATGTGAT+AGG | - | 51722:44795-44814 | None:intergenic | 45.0% | |
| GTGTGGCATACCCTTATGAA+CGG | - | 51722:44837-44856 | None:intergenic | 45.0% | |
| TAACATGCTCCACTCGAACA+TGG | - | 51722:45550-45569 | None:intergenic | 45.0% | |
| TAAGGCTGATGCTAAGGCAA+AGG | + | 51722:45625-45644 | MS.gene007018:CDS | 45.0% | |
| TCGGCGATTATGTCGATATC+AGG | + | 51722:44801-44820 | MS.gene007018:CDS | 45.0% | |
| TCTCGTCCATTCAGGAAGAA+GGG | + | 51722:44740-44759 | MS.gene007018:CDS | 45.0% | |
| TGCCACACAAGTTCTACCAT+GGG | + | 51722:44849-44868 | MS.gene007018:CDS | 45.0% | |
| TGGAGCATGTTATGCCATCT+AGG | + | 51722:45558-45577 | MS.gene007018:CDS | 45.0% | |
| !! | CAAACGTGCTATTGGTGTTG+AGG | + | 51722:44898-44917 | MS.gene007018:CDS | 45.0% |
| !! | GTGATAGGTTCTGAGGTAAG+TGG | - | 51722:44780-44799 | None:intergenic | 45.0% |
| !! | GTTAACGGTGCCGTTCATAA+GGG | + | 51722:44824-44843 | MS.gene007018:CDS | 45.0% |
| AAGCCAGAAGGACCTAAACC+TGG | + | 51722:45671-45690 | MS.gene007018:CDS | 50.0% | |
| AAGTTCTACCATGGGCGTAC+CGG | + | 51722:44857-44876 | MS.gene007018:CDS | 50.0% | |
| CTCTCGTCCATTCAGGAAGA+AGG | + | 51722:44739-44758 | MS.gene007018:CDS | 50.0% | |
| CTTCCTCAAACGGAACTCCT+CGG | - | 51722:45588-45607 | None:intergenic | 50.0% | |
| GATCTCAAACCATGACCAGC+CGG | - | 51722:44701-44720 | None:intergenic | 50.0% | |
| GCTCCTTCAACCATGAAACC+AGG | - | 51722:45692-45711 | None:intergenic | 50.0% | |
| !! | GGTTAACGGTGCCGTTCATA+AGG | + | 51722:44823-44842 | MS.gene007018:CDS | 50.0% |
| CGCCCATGGTAGAACTTGTG+TGG | - | 51722:44854-44873 | None:intergenic | 55.0% | |
| CGCCGATGTGATAGGTTCTG+AGG | - | 51722:44787-44806 | None:intergenic | 55.0% | |
| GGTAACGTTCCAGACACGAC+CGG | - | 51722:44879-44898 | None:intergenic | 55.0% | |
| TATGCCATCTAGGTGCACCG+AGG | + | 51722:45568-45587 | MS.gene007018:CDS | 55.0% | |
| ! | AACTCCTCGGTGCACCTAGA+TGG | - | 51722:45575-45594 | None:intergenic | 55.0% |
| !! | AGGTTCTGAGGTAAGTGGCG+AGG | - | 51722:44775-44794 | None:intergenic | 55.0% |
| !! | GGTTCTGAGGTAAGTGGCGA+GGG | - | 51722:44774-44793 | None:intergenic | 55.0% |
| AGCACCAAGCGCAAGCCAGA+AGG | + | 51722:45659-45678 | MS.gene007018:CDS | 60.0% | |
| GCACCGAGGAGTTCCGTTTG+AGG | + | 51722:45582-45601 | MS.gene007018:CDS | 60.0% | |
| !! | AGGTCCTTCTGGCTTGCGCT+TGG | - | 51722:45666-45685 | None:intergenic | 60.0% |
| AGACACGACCGGTACGCCCA+TGG | - | 51722:44868-44887 | None:intergenic | 65.0% | |
| ! | ATGGGCGTACCGGTCGTGTC+TGG | + | 51722:44867-44886 | MS.gene007018:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51722 | gene | 44695 | 45775 | 44695 | ID=MS.gene007018 |
| 51722 | mRNA | 44695 | 45775 | 44695 | ID=MS.gene007018.t1;Parent=MS.gene007018 |
| 51722 | exon | 44695 | 44931 | 44695 | ID=MS.gene007018.t1.exon1;Parent=MS.gene007018.t1 |
| 51722 | CDS | 44695 | 44931 | 44695 | ID=cds.MS.gene007018.t1;Parent=MS.gene007018.t1 |
| 51722 | exon | 45518 | 45775 | 45518 | ID=MS.gene007018.t1.exon2;Parent=MS.gene007018.t1 |
| 51722 | CDS | 45518 | 45775 | 45518 | ID=cds.MS.gene007018.t1;Parent=MS.gene007018.t1 |
| Gene Sequence |
| Protein sequence |
