AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50640


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50640.t1 MTR_1g069905 100.000 164 0 0 1 164 1 164 1.48e-120 337
MS.gene50640.t1 MTR_7g107420 99.390 164 1 0 1 164 1 164 2.29e-120 336
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50640.t1 AT1G09690 81.098 164 31 0 1 164 1 164 3.44e-101 288
MS.gene50640.t1 AT1G09590 81.098 164 31 0 1 164 1 164 3.44e-101 288
MS.gene50640.t1 AT1G57860 80.488 164 32 0 1 164 1 164 1.04e-100 286
MS.gene50640.t1 AT1G57660 80.488 164 32 0 1 164 1 164 1.04e-100 286

Find 61 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGTCGACATTAGAGTTAA+TGG 0.335175 1.2:+53134904 MS.gene50640:CDS
TCCACCATGAAACCAGGTTT+GGG 0.345744 1.2:-53136049 None:intergenic
AACTCCTCAGTGCACCTTGA+TGG 0.353770 1.2:-53135938 None:intergenic
AGATCATTAACGACATCATA+TGG 0.366035 1.2:-53136103 None:intergenic
GGGGCCCTCTGGCTTGCGCT+TGG 0.369033 1.2:-53136029 None:intergenic
TTCCACCATGAAACCAGGTT+TGG 0.392778 1.2:-53136050 None:intergenic
GGGCCCCAAACCTGGTTTCA+TGG 0.399375 1.2:+53136045 MS.gene50640:CDS
TTAACGACATCATATGGAAT+TGG 0.408404 1.2:-53136097 None:intergenic
CTTGTTGACTTCGACACCAA+TGG 0.410846 1.2:-53135001 None:intergenic
AAACCAGGTTTGGGGCCCTC+TGG 0.418683 1.2:-53136040 None:intergenic
ACGGTCGTACCGGTCGTGTC+TGG 0.430669 1.2:+53134962 MS.gene50640:CDS
AAGCCAGAGGGCCCCAAACC+TGG 0.445982 1.2:+53136037 MS.gene50640:CDS
CTCTCCCTTGGCCTTAGCCT+CGG 0.448320 1.2:-53135996 None:intergenic
GGTGCTTATCACCTCTCCCT+TGG 0.453810 1.2:-53136008 None:intergenic
CTCTCGTCCCTTCCGCAAGA+AGG 0.461875 1.2:+53134834 MS.gene50640:CDS
TTCGACACCAATGGCCCGCT+TGG 0.477602 1.2:-53134992 None:intergenic
AGTCCTGAGGTAGGTGGCTA+AGG 0.478683 1.2:-53134866 None:intergenic
CGAGGCTAAGGCCAAGGGAG+AGG 0.486454 1.2:+53135997 MS.gene50640:CDS
GGGACGAGAGAATGAATCTC+TGG 0.494698 1.2:-53134821 None:intergenic
AGCACCAAGCGCAAGCCAGA+GGG 0.504695 1.2:+53136025 MS.gene50640:CDS
TATTGTTCCCTTCTTGCGGA+AGG 0.508429 1.2:-53134842 None:intergenic
TCAATTGAAGGCCGAGGCTA+AGG 0.510816 1.2:+53135985 MS.gene50640:CDS
AATGTCACCAAGCGGGCCAT+TGG 0.521717 1.2:+53134985 MS.gene50640:CDS
AAATTCTACCACGGTCGTAC+CGG 0.524822 1.2:+53134952 MS.gene50640:CDS
GATCAAGAATGATCAATTGA+AGG 0.525684 1.2:+53135973 MS.gene50640:CDS
GAAAACATGCCTGCAGGTCA+TGG 0.528675 1.2:+53134784 None:intergenic
GATCGTAAACCATGACCTGC+AGG 0.529368 1.2:-53134793 None:intergenic
CATGGTGGAAGGTGCTACAT+TGG 0.534415 1.2:+53136063 MS.gene50640:CDS
ATTGTTCCCTTCTTGCGGAA+GGG 0.538504 1.2:-53134841 None:intergenic
AGAGAATGAATCTCTGGTGC+GGG 0.544173 1.2:-53134815 None:intergenic
TCTCGTCCCTTCCGCAAGAA+GGG 0.545142 1.2:+53134835 MS.gene50640:CDS
TAGCCTTAGCCACCTACCTC+AGG 0.549870 1.2:+53134863 MS.gene50640:CDS
GAATGATCAATTGAAGGCCG+AGG 0.551548 1.2:+53135979 MS.gene50640:CDS
AGGCTATTGTTCCCTTCTTG+CGG 0.554250 1.2:-53134846 None:intergenic
AAGCACCAAGCGCAAGCCAG+AGG 0.557087 1.2:+53136024 MS.gene50640:CDS
GAAGAAGAAAACATGCCTGC+AGG 0.557795 1.2:+53134778 None:intergenic
TGGAGCATGTTATGCCATCA+AGG 0.559210 1.2:+53135924 MS.gene50640:CDS
GAAGGCCGAGGCTAAGGCCA+AGG 0.560874 1.2:+53135991 MS.gene50640:CDS
TGTCTGGAATGTCACCAAGC+GGG 0.572072 1.2:+53134978 MS.gene50640:CDS
GCACCTTCCACCATGAAACC+AGG 0.576804 1.2:-53136055 None:intergenic
AAACCTGGTTTCATGGTGGA+AGG 0.589005 1.2:+53136052 MS.gene50640:CDS
GCACTGAGGAGTTCAGATTG+AGG 0.599052 1.2:+53135948 MS.gene50640:CDS
GGTGACATTCCAGACACGAC+CGG 0.600537 1.2:-53134971 None:intergenic
ATGGCATAACATGCTCCACT+CGG 0.618716 1.2:-53135919 None:intergenic
GAGAGAATGAATCTCTGGTG+CGG 0.619187 1.2:-53134816 None:intergenic
GATGTCGTTAATGATCTCAA+AGG 0.621805 1.2:+53136109 MS.gene50640:CDS
GTTAATGGAGCTGTGCACAA+AGG 0.632022 1.2:+53134919 MS.gene50640:CDS
GTCGTTAATGATCTCAAAGG+AGG 0.636972 1.2:+53136112 MS.gene50640:CDS
AAGGCCGAGGCTAAGGCCAA+GGG 0.643263 1.2:+53135992 MS.gene50640:CDS
CCACCATGAAACCAGGTTTG+GGG 0.653619 1.2:-53136048 None:intergenic
GTGTCTGGAATGTCACCAAG+CGG 0.655012 1.2:+53134977 MS.gene50640:CDS
GTGATAAGTCCTGAGGTAGG+TGG 0.662852 1.2:-53134872 None:intergenic
CGACCGTGGTAGAATTTGTG+CGG 0.677873 1.2:-53134946 None:intergenic
CACCGATGTGATAAGTCCTG+AGG 0.683027 1.2:-53134879 None:intergenic
GATGTGATAAGTCCTGAGGT+AGG 0.683685 1.2:-53134875 None:intergenic
CCCCAAACCTGGTTTCATGG+TGG 0.684233 1.2:+53136048 MS.gene50640:CDS
AGACACGACCGGTACGACCG+TGG 0.690324 1.2:-53134960 None:intergenic
GAAGAGGATTCATGTCCGAG+TGG 0.705446 1.2:+53135904 MS.gene50640:CDS
TACCTCAGGACTTATCACAT+CGG 0.727991 1.2:+53134877 MS.gene50640:CDS
ATGCCGCACAAATTCTACCA+CGG 0.741255 1.2:+53134943 MS.gene50640:CDS
TATGCCATCAAGGTGCACTG+AGG 0.765204 1.2:+53135934 MS.gene50640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACATAAATGAAAGTATTTGT+AGG - chr1.2:53135539-53135558 None:intergenic 20.0%
!!! CAAAATTCTTCTAAGTTTTT+AGG + chr1.2:53135691-53135710 MS.gene50640:intron 20.0%
!!! GAAGAATTTTGTGTTAAATA+TGG - chr1.2:53135682-53135701 None:intergenic 20.0%
! AAGTTGGAAAACAAAAACTT+AGG - chr1.2:53135459-53135478 None:intergenic 25.0%
! AGGAACTAAACATGATAAAA+TGG - chr1.2:53135519-53135538 None:intergenic 25.0%
! AGTTGGAAAACAAAAACTTA+GGG - chr1.2:53135458-53135477 None:intergenic 25.0%
! TCAAACAACAAAATGAATCA+AGG - chr1.2:53135265-53135284 None:intergenic 25.0%
! TTTGCTTTAGTAGTATATTC+AGG + chr1.2:53135187-53135206 MS.gene50640:intron 25.0%
!! ACTTGACATGATGTTTTATA+TGG + chr1.2:53135649-53135668 MS.gene50640:intron 25.0%
!! ATTCGAGTCTTTTAATAAGT+TGG - chr1.2:53135475-53135494 None:intergenic 25.0%
!!! AATGTTGCTATTTATGTTTC+AGG + chr1.2:53135862-53135881 MS.gene50640:intron 25.0%
!!! TTCTAAGTTTTTAGGTCTTA+AGG + chr1.2:53135699-53135718 MS.gene50640:intron 25.0%
!!! TTGTTTGAGTTTTGTTTAGA+TGG + chr1.2:53135277-53135296 MS.gene50640:intron 25.0%
AATCTCTTGGATAGAATGTA+TGG + chr1.2:53135759-53135778 MS.gene50640:intron 30.0%
ACAAATATATGTGACGTGAT+AGG + chr1.2:53135079-53135098 MS.gene50640:intron 30.0%
AGATCATTAACGACATCATA+TGG - chr1.2:53136106-53136125 None:intergenic 30.0%
ATAAGCAGTAAGCTATCTTA+GGG - chr1.2:53135332-53135351 None:intergenic 30.0%
CAATATCTTTACAAGCAAAG+CGG - chr1.2:53135827-53135846 None:intergenic 30.0%
CATCAAAATCACCATATATG+AGG + chr1.2:53135610-53135629 MS.gene50640:intron 30.0%
CTCTTCAAAAACCTCATATA+TGG - chr1.2:53135624-53135643 None:intergenic 30.0%
GATCAAGAATGATCAATTGA+AGG + chr1.2:53135973-53135992 MS.gene50640:CDS 30.0%
TATAAGCAGTAAGCTATCTT+AGG - chr1.2:53135333-53135352 None:intergenic 30.0%
TATGTCGACATTAGAGTTAA+TGG + chr1.2:53134904-53134923 MS.gene50640:CDS 30.0%
TTAACGACATCATATGGAAT+TGG - chr1.2:53136100-53136119 None:intergenic 30.0%
TTAATTTCCAGAGAATCTCT+TGG + chr1.2:53135746-53135765 MS.gene50640:intron 30.0%
!! TGCTATTTATGTTTCAGGTT+AGG + chr1.2:53135867-53135886 MS.gene50640:intron 30.0%
!!! TGAGTTTTGTTTAGATGGAT+TGG + chr1.2:53135282-53135301 MS.gene50640:intron 30.0%
ACAAAAACTTAGGGCATGTT+TGG - chr1.2:53135449-53135468 None:intergenic 35.0%
AGTAAGCTATCTTAGGGATA+AGG - chr1.2:53135326-53135345 None:intergenic 35.0%
ATTCTATCCAAGAGATTCTC+TGG - chr1.2:53135756-53135775 None:intergenic 35.0%
GATGTCGTTAATGATCTCAA+AGG + chr1.2:53136109-53136128 MS.gene50640:CDS 35.0%
TCCAATTCCAAGTGAGAAAT+TGG - chr1.2:53135569-53135588 None:intergenic 35.0%
TCCAATTTCTCACTTGGAAT+TGG + chr1.2:53135565-53135584 MS.gene50640:intron 35.0%
! AGGTTAGGAACAAGATTTTG+AGG + chr1.2:53135882-53135901 MS.gene50640:intron 35.0%
ATGTGACGTGATAGGTGTTA+TGG + chr1.2:53135087-53135106 MS.gene50640:intron 40.0%
CAAGTCTCCAATTTCTCACT+TGG + chr1.2:53135559-53135578 MS.gene50640:intron 40.0%
GTCGTTAATGATCTCAAAGG+AGG + chr1.2:53136112-53136131 MS.gene50640:CDS 40.0%
TACCTCAGGACTTATCACAT+CGG + chr1.2:53134877-53134896 MS.gene50640:CDS 40.0%
!! GGAACAAGATTTTGAGGAAG+AGG + chr1.2:53135888-53135907 MS.gene50640:CDS 40.0%
AAATTCTACCACGGTCGTAC+CGG + chr1.2:53134952-53134971 MS.gene50640:CDS 45.0%
AGAGAATGAATCTCTGGTGC+GGG - chr1.2:53134818-53134837 None:intergenic 45.0%
AGGCTATTGTTCCCTTCTTG+CGG - chr1.2:53134849-53134868 None:intergenic 45.0%
ATGCCGCACAAATTCTACCA+CGG + chr1.2:53134943-53134962 MS.gene50640:CDS 45.0%
ATGGCATAACATGCTCCACT+CGG - chr1.2:53135922-53135941 None:intergenic 45.0%
ATTGTTCCCTTCTTGCGGAA+GGG - chr1.2:53134844-53134863 None:intergenic 45.0%
GAATGATCAATTGAAGGCCG+AGG + chr1.2:53135979-53135998 MS.gene50640:CDS 45.0%
GAGAGAATGAATCTCTGGTG+CGG - chr1.2:53134819-53134838 None:intergenic 45.0%
GATGTGATAAGTCCTGAGGT+AGG - chr1.2:53134878-53134897 None:intergenic 45.0%
GTTAATGGAGCTGTGCACAA+AGG + chr1.2:53134919-53134938 MS.gene50640:CDS 45.0%
TATTGTTCCCTTCTTGCGGA+AGG - chr1.2:53134845-53134864 None:intergenic 45.0%
TCCACCATGAAACCAGGTTT+GGG - chr1.2:53136052-53136071 None:intergenic 45.0%
TGGAGCATGTTATGCCATCA+AGG + chr1.2:53135924-53135943 MS.gene50640:CDS 45.0%
TTCCACCATGAAACCAGGTT+TGG - chr1.2:53136053-53136072 None:intergenic 45.0%
! AAACCTGGTTTCATGGTGGA+AGG + chr1.2:53136052-53136071 MS.gene50640:CDS 45.0%
!! CTTGTTGACTTCGACACCAA+TGG - chr1.2:53135004-53135023 None:intergenic 45.0%
AACTCCTCAGTGCACCTTGA+TGG - chr1.2:53135941-53135960 None:intergenic 50.0%
CACCGATGTGATAAGTCCTG+AGG - chr1.2:53134882-53134901 None:intergenic 50.0%
CCACCATGAAACCAGGTTTG+GGG - chr1.2:53136051-53136070 None:intergenic 50.0%
CGACCGTGGTAGAATTTGTG+CGG - chr1.2:53134949-53134968 None:intergenic 50.0%
GAAGAGGATTCATGTCCGAG+TGG + chr1.2:53135904-53135923 MS.gene50640:CDS 50.0%
GATCGTAAACCATGACCTGC+AGG - chr1.2:53134796-53134815 None:intergenic 50.0%
GCACTGAGGAGTTCAGATTG+AGG + chr1.2:53135948-53135967 MS.gene50640:CDS 50.0%
GGGACGAGAGAATGAATCTC+TGG - chr1.2:53134824-53134843 None:intergenic 50.0%
GTGATAAGTCCTGAGGTAGG+TGG - chr1.2:53134875-53134894 None:intergenic 50.0%
TATGCCATCAAGGTGCACTG+AGG + chr1.2:53135934-53135953 MS.gene50640:CDS 50.0%
TCAATTGAAGGCCGAGGCTA+AGG + chr1.2:53135985-53136004 MS.gene50640:CDS 50.0%
! GTGTCTGGAATGTCACCAAG+CGG + chr1.2:53134977-53134996 MS.gene50640:CDS 50.0%
! TGTCTGGAATGTCACCAAGC+GGG + chr1.2:53134978-53134997 MS.gene50640:CDS 50.0%
!! CATGGTGGAAGGTGCTACAT+TGG + chr1.2:53136063-53136082 MS.gene50640:CDS 50.0%
AATGTCACCAAGCGGGCCAT+TGG + chr1.2:53134985-53135004 MS.gene50640:CDS 55.0%
AGTCCTGAGGTAGGTGGCTA+AGG - chr1.2:53134869-53134888 None:intergenic 55.0%
CCCCAAACCTGGTTTCATGG+TGG + chr1.2:53136048-53136067 MS.gene50640:CDS 55.0%
GCACCTTCCACCATGAAACC+AGG - chr1.2:53136058-53136077 None:intergenic 55.0%
GGTGACATTCCAGACACGAC+CGG - chr1.2:53134974-53134993 None:intergenic 55.0%
GGTGCTTATCACCTCTCCCT+TGG - chr1.2:53136011-53136030 None:intergenic 55.0%
TCTCGTCCCTTCCGCAAGAA+GGG + chr1.2:53134835-53134854 MS.gene50640:CDS 55.0%
! TAGCCTTAGCCACCTACCTC+AGG + chr1.2:53134863-53134882 MS.gene50640:CDS 55.0%
AAGCACCAAGCGCAAGCCAG+AGG + chr1.2:53136024-53136043 MS.gene50640:CDS 60.0%
AAGGCCGAGGCTAAGGCCAA+GGG + chr1.2:53135992-53136011 MS.gene50640:CDS 60.0%
AGCACCAAGCGCAAGCCAGA+GGG + chr1.2:53136025-53136044 MS.gene50640:CDS 60.0%
CTCTCGTCCCTTCCGCAAGA+AGG + chr1.2:53134834-53134853 MS.gene50640:CDS 60.0%
GGGCCCCAAACCTGGTTTCA+TGG + chr1.2:53136045-53136064 MS.gene50640:CDS 60.0%
TTCGACACCAATGGCCCGCT+TGG - chr1.2:53134995-53135014 None:intergenic 60.0%
! AAACCAGGTTTGGGGCCCTC+TGG - chr1.2:53136043-53136062 None:intergenic 60.0%
!! CTCTCCCTTGGCCTTAGCCT+CGG - chr1.2:53135999-53136018 None:intergenic 60.0%
AAGCCAGAGGGCCCCAAACC+TGG + chr1.2:53136037-53136056 MS.gene50640:CDS 65.0%
AGACACGACCGGTACGACCG+TGG - chr1.2:53134963-53134982 None:intergenic 65.0%
CGAGGCTAAGGCCAAGGGAG+AGG + chr1.2:53135997-53136016 MS.gene50640:CDS 65.0%
GAAGGCCGAGGCTAAGGCCA+AGG + chr1.2:53135991-53136010 MS.gene50640:CDS 65.0%
! ACGGTCGTACCGGTCGTGTC+TGG + chr1.2:53134962-53134981 MS.gene50640:CDS 65.0%
! GGGGCCCTCTGGCTTGCGCT+TGG - chr1.2:53136032-53136051 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 53134790 53136141 53134790 ID=MS.gene50640
chr1.2 mRNA 53134790 53136141 53134790 ID=MS.gene50640.t1;Parent=MS.gene50640
chr1.2 exon 53134790 53135026 53134790 ID=MS.gene50640.t1.exon1;Parent=MS.gene50640.t1
chr1.2 CDS 53134790 53135026 53134790 ID=cds.MS.gene50640.t1;Parent=MS.gene50640.t1
chr1.2 exon 53135884 53136141 53135884 ID=MS.gene50640.t1.exon2;Parent=MS.gene50640.t1
chr1.2 CDS 53135884 53136141 53135884 ID=cds.MS.gene50640.t1;Parent=MS.gene50640.t1
Gene Sequence

>MS.gene50640

ATGCCTGCAGGTCATGGTTTACGATCCCGCACCAGAGATTCATTCTCTCGTCCCTTCCGCAAGAAGGGAACAATAGCCTTAGCCACCTACCTCAGGACTTATCACATCGGTGACTATGTCGACATTAGAGTTAATGGAGCTGTGCACAAAGGTATGCCGCACAAATTCTACCACGGTCGTACCGGTCGTGTCTGGAATGTCACCAAGCGGGCCATTGGTGTCGAAGTCAACAAGCAAGTATGTCAAATCTTCTTCTTATATGATTCATTTTCATTTTAGTGTTATATCTACAAATATATGTGACGTGATAGGTGTTATGGTTTGTTCGATTGATATTTTTTTTGTTTCATTTCGAGCTTTCTTTTGAATTGTTATGTATTTGTTGCAAATATGTTTCTTTGCTTTAGTAGTATATTCAGGATCTAGTTTAAGTTGTTCATGTTACAAATGAATTGTTTATTGTTGTTTCATTCCTTGATTCATTTTGTTGTTTGAGTTTTGTTTAGATGGATTGGCTTCTGAGTTTTCAGAAGCCTTATCCCTAAGATAGCTTACTGCTTATAGCATATACAGAAACAATTCAATTTTATTTTATCATTTGCAGCAAAAATAAGCTCTTATCATGACAATAAGCACTTGTGCTATAAGCTGTCTATCCAAACATGCCCTAAGTTTTTGTTTTCCAACTTATTAAAAGACTCGAATTTTGACTCATATGTACTAGTACCATTTTATCATGTTTAGTTCCTACAAATACTTTCATTTATGTCAAGTCTCCAATTTCTCACTTGGAATTGGAACAATTATTTATGAAAATTTTCATCAAAATCACCATATATGAGGTTTTTGAAGAGACTGAACTTGACATGATGTTTTATATGGACTCGAACCATATTTAACACAAAATTCTTCTAAGTTTTTAGGTCTTAAGGTAAATGAGATTGAGACTAACATTTTTAATTTCCAGAGAATCTCTTGGATAGAATGTATGGTTGTTGCATAATTATCTCAATTTTTTATGTTCTCTGCAAGTGCCGCTTTGCTTGTAAAGATATTGTTTACTCAATTTTAAAATGTTGCTATTTATGTTTCAGGTTAGGAACAAGATTTTGAGGAAGAGGATTCATGTCCGAGTGGAGCATGTTATGCCATCAAGGTGCACTGAGGAGTTCAGATTGAGGAAGATCAAGAATGATCAATTGAAGGCCGAGGCTAAGGCCAAGGGAGAGGTGATAAGCACCAAGCGCAAGCCAGAGGGCCCCAAACCTGGTTTCATGGTGGAAGGTGCTACATTGGAGACTGTTACTCCAATTCCATATGATGTCGTTAATGATCTCAAAGGAGGATACTAG

Protein sequence

>MS.gene50640.t1

MPAGHGLRSRTRDSFSRPFRKKGTIALATYLRTYHIGDYVDIRVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEVNKQVRNKILRKRIHVRVEHVMPSRCTEEFRLRKIKNDQLKAEAKAKGEVISTKRKPEGPKPGFMVEGATLETVTPIPYDVVNDLKGGY