Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23031.t1 | XP_013468487.1 | 100 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 2.40E-88 | 334.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23031.t1 | Q43291 | 81.1 | 164 | 31 | 0 | 1 | 164 | 1 | 164 | 1.0e-76 | 287.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23031.t1 | I3T8M7 | 100.0 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 1.7e-88 | 334.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene23031 | 0.818965 | 1.49E-52 | -1.69E-46 |
| MS.gene050134 | MS.gene23031 | 0.879842 | 8.96E-70 | -1.69E-46 |
| MS.gene050252 | MS.gene23031 | 0.81988 | 9.21E-53 | -1.69E-46 |
| MS.gene050791 | MS.gene23031 | 0.868819 | 4.92E-66 | -1.69E-46 |
| MS.gene050912 | MS.gene23031 | 0.818544 | 1.85E-52 | -1.69E-46 |
| MS.gene051904 | MS.gene23031 | 0.826771 | 2.26E-54 | -1.69E-46 |
| MS.gene051984 | MS.gene23031 | 0.81768 | 2.91E-52 | -1.69E-46 |
| MS.gene052664 | MS.gene23031 | 0.876582 | 1.24E-68 | -1.69E-46 |
| MS.gene052666 | MS.gene23031 | 0.826464 | 2.67E-54 | -1.69E-46 |
| MS.gene053224 | MS.gene23031 | 0.857156 | 1.98E-62 | -1.69E-46 |
| MS.gene057272 | MS.gene23031 | 0.855175 | 7.52E-62 | -1.69E-46 |
| MS.gene057641 | MS.gene23031 | 0.901781 | 1.89E-78 | -1.69E-46 |
| MS.gene057643 | MS.gene23031 | 0.916446 | 1.76E-85 | -1.69E-46 |
| MS.gene060432 | MS.gene23031 | 0.855382 | 6.55E-62 | -1.69E-46 |
| MS.gene060483 | MS.gene23031 | 0.833651 | 4.72E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene23031 | MS.gene007018 | PPI |
| MS.gene79286 | MS.gene23031 | PPI |
| MS.gene000360 | MS.gene23031 | PPI |
| MS.gene50640 | MS.gene23031 | PPI |
| MS.gene036076 | MS.gene23031 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23031.t1 | MTR_1g069905 | 100.000 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 1.48e-120 | 337 |
| MS.gene23031.t1 | MTR_7g107420 | 99.390 | 164 | 1 | 0 | 1 | 164 | 1 | 164 | 2.29e-120 | 336 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23031.t1 | AT1G09690 | 81.098 | 164 | 31 | 0 | 1 | 164 | 1 | 164 | 3.44e-101 | 288 |
| MS.gene23031.t1 | AT1G09590 | 81.098 | 164 | 31 | 0 | 1 | 164 | 1 | 164 | 3.44e-101 | 288 |
| MS.gene23031.t1 | AT1G57860 | 80.488 | 164 | 32 | 0 | 1 | 164 | 1 | 164 | 1.04e-100 | 286 |
| MS.gene23031.t1 | AT1G57660 | 80.488 | 164 | 32 | 0 | 1 | 164 | 1 | 164 | 1.04e-100 | 286 |
Find 58 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCTTTCTCTCGTCCATTC+AGG | 0.293911 | 7.2:+6961869 | MS.gene23031:CDS |
| AAACCAGGTTTAGGTCCTTC+TGG | 0.327783 | 7.2:-6962823 | None:intergenic |
| TCGGCGATTATGTCGATATC+AGG | 0.353109 | 7.2:+6961938 | MS.gene23031:CDS |
| AGGACCTAAACCTGGTTTCA+TGG | 0.367201 | 7.2:+6962828 | MS.gene23031:CDS |
| TGATGTGGTTAATGATCTTA+AGG | 0.381458 | 7.2:+6962891 | MS.gene23031:CDS |
| GGTTAACGGTGCCGTTCATA+AGG | 0.392360 | 7.2:+6961960 | MS.gene23031:CDS |
| TCAACCATGAAACCAGGTTT+AGG | 0.394105 | 7.2:-6962832 | None:intergenic |
| TTAACCACATCATATGGAAT+TGG | 0.408404 | 7.2:-6962880 | None:intergenic |
| CTCAACACCAATAGCACGTT+TGG | 0.409541 | 7.2:-6962034 | None:intergenic |
| CTCTCGTCCATTCAGGAAGA+AGG | 0.411770 | 7.2:+6961876 | MS.gene23031:CDS |
| AACTCCTCGGTGCACCTAGA+TGG | 0.414621 | 7.2:-6962721 | None:intergenic |
| GACATAATCGCCGATGTGAT+AGG | 0.419105 | 7.2:-6961929 | None:intergenic |
| AGATCATTAACCACATCATA+TGG | 0.425684 | 7.2:-6962886 | None:intergenic |
| ATGGGCGTACCGGTCGTGTC+TGG | 0.430669 | 7.2:+6962004 | MS.gene23031:CDS |
| ATTGTACCCTTCTTCCTGAA+TGG | 0.450869 | 7.2:-6961883 | None:intergenic |
| AGGTTAGGAACAAGATTCTC+AGG | 0.451952 | 7.2:+6962665 | MS.gene23031:intron |
| AAACCTGGTTTCATGGTTGA+AGG | 0.456376 | 7.2:+6962835 | MS.gene23031:CDS |
| CATTCTTAATCTTCCTCAAA+CGG | 0.463824 | 7.2:-6962744 | None:intergenic |
| AACGTTACCAAACGTGCTAT+TGG | 0.472288 | 7.2:+6962027 | MS.gene23031:CDS |
| AGGTCCTTCTGGCTTGCGCT+TGG | 0.474164 | 7.2:-6962812 | None:intergenic |
| GATTAAGAATGATCAACTTA+AGG | 0.476396 | 7.2:+6962756 | MS.gene23031:CDS |
| TGGAGCATGTTATGCCATCT+AGG | 0.488269 | 7.2:+6962707 | MS.gene23031:CDS |
| TATGTCGATATCAGGGTTAA+CGG | 0.499321 | 7.2:+6961946 | MS.gene23031:CDS |
| GATGTGGTTAATGATCTTAA+GGG | 0.514482 | 7.2:+6962892 | MS.gene23031:CDS |
| CATGGTTGAAGGAGCTACAT+TGG | 0.515633 | 7.2:+6962846 | MS.gene23031:CDS |
| CAAACGTGCTATTGGTGTTG+AGG | 0.526075 | 7.2:+6962035 | MS.gene23031:CDS |
| TCAACTTAAGGCTGAGGCTA+AGG | 0.526123 | 7.2:+6962768 | MS.gene23031:CDS |
| AGAAAGATGCCGGCTGGTCA+TGG | 0.530667 | 7.2:+6961826 | None:intergenic |
| GCACCGAGGAGTTCCGTTTG+AGG | 0.535387 | 7.2:+6962731 | MS.gene23031:CDS |
| AAGTTCTACCATGGGCGTAC+CGG | 0.536603 | 7.2:+6961994 | MS.gene23031:CDS |
| GAATGATCAACTTAAGGCTG+AGG | 0.542856 | 7.2:+6962762 | MS.gene23031:CDS |
| GTTAACGGTGCCGTTCATAA+GGG | 0.556218 | 7.2:+6961961 | MS.gene23031:CDS |
| GATCTCAAACCATGACCAGC+CGG | 0.563821 | 7.2:-6961835 | None:intergenic |
| AAGGCTGAGGCTAAGGCAAA+GGG | 0.569105 | 7.2:+6962775 | MS.gene23031:CDS |
| TAAGGCTGAGGCTAAGGCAA+AGG | 0.574426 | 7.2:+6962774 | MS.gene23031:CDS |
| CGCCGATGTGATAGGTTCTG+AGG | 0.582425 | 7.2:-6961921 | None:intergenic |
| TAACATGCTCCACTCGAACA+TGG | 0.582558 | 7.2:-6962696 | None:intergenic |
| AGGAGAAGAAAGATGCCGGC+TGG | 0.584433 | 7.2:+6961820 | None:intergenic |
| CTTCCTCAAACGGAACTCCT+CGG | 0.593955 | 7.2:-6962734 | None:intergenic |
| AGCACCAAGCGCAAGCCAGA+AGG | 0.595144 | 7.2:+6962808 | MS.gene23031:CDS |
| TACTCCAATTCCATATGATG+TGG | 0.596403 | 7.2:+6962876 | MS.gene23031:CDS |
| TCTCGTCCATTCAGGAAGAA+GGG | 0.597777 | 7.2:+6961877 | MS.gene23031:CDS |
| GTGATAGGTTCTGAGGTAAG+TGG | 0.598261 | 7.2:-6961914 | None:intergenic |
| GGTAACGTTCCAGACACGAC+CGG | 0.599652 | 7.2:-6962013 | None:intergenic |
| GGTTCTGAGGTAAGTGGCGA+GGG | 0.600826 | 7.2:-6961908 | None:intergenic |
| CGCCCATGGTAGAACTTGTG+TGG | 0.607884 | 7.2:-6961988 | None:intergenic |
| GCTCCTTCAACCATGAAACC+AGG | 0.609967 | 7.2:-6962838 | None:intergenic |
| TGCCACACAAGTTCTACCAT+GGG | 0.615951 | 7.2:+6961986 | MS.gene23031:CDS |
| AGGTTCTGAGGTAAGTGGCG+AGG | 0.615968 | 7.2:-6961909 | None:intergenic |
| AGACACGACCGGTACGCCCA+TGG | 0.619384 | 7.2:-6962002 | None:intergenic |
| ATGCCACACAAGTTCTACCA+TGG | 0.619578 | 7.2:+6961985 | MS.gene23031:CDS |
| CGGCGATTATGTCGATATCA+GGG | 0.624782 | 7.2:+6961939 | MS.gene23031:CDS |
| AAGCCAGAAGGACCTAAACC+TGG | 0.625100 | 7.2:+6962820 | MS.gene23031:CDS |
| GTGGTTAATGATCTTAAGGG+AGG | 0.627017 | 7.2:+6962895 | MS.gene23031:CDS |
| GTGTGGCATACCCTTATGAA+CGG | 0.627528 | 7.2:-6961971 | None:intergenic |
| GAAGAGAATCCATGTTCGAG+TGG | 0.642302 | 7.2:+6962687 | MS.gene23031:CDS |
| TACCTCAGAACCTATCACAT+CGG | 0.680253 | 7.2:+6961919 | MS.gene23031:CDS |
| TATGCCATCTAGGTGCACCG+AGG | 0.784253 | 7.2:+6962717 | MS.gene23031:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTGATTTATTGTATTTTA+GGG | + | chr7.2:6962178-6962197 | MS.gene23031:intron | 10.0% |
| !!! | TTATTGATTTATTGTATTTT+AGG | + | chr7.2:6962177-6962196 | MS.gene23031:intron | 10.0% |
| !! | AAATGAATTAAGTAAGTATT+TGG | + | chr7.2:6962359-6962378 | MS.gene23031:intron | 15.0% |
| !! | ATATAATTACATTGATCAAA+TGG | - | chr7.2:6962543-6962562 | None:intergenic | 15.0% |
| !!! | ATTATATGTTGTTATTTATC+TGG | + | chr7.2:6962556-6962575 | MS.gene23031:intron | 15.0% |
| !!! | TATTGAAATATTGCTATTTG+TGG | + | chr7.2:6962629-6962648 | MS.gene23031:intron | 20.0% |
| ! | AAAACCACAGAACTAAAATT+CGG | - | chr7.2:6962097-6962116 | None:intergenic | 25.0% |
| ! | GATTAAGAATGATCAACTTA+AGG | + | chr7.2:6962756-6962775 | MS.gene23031:CDS | 25.0% |
| !! | GGATTTTGTAAATTTACTTG+TGG | + | chr7.2:6962380-6962399 | MS.gene23031:intron | 25.0% |
| !! | TGTGTGCTTTAATTACATTT+TGG | + | chr7.2:6962223-6962242 | MS.gene23031:intron | 25.0% |
| AGATCATTAACCACATCATA+TGG | - | chr7.2:6962889-6962908 | None:intergenic | 30.0% | |
| CATTCTTAATCTTCCTCAAA+CGG | - | chr7.2:6962747-6962766 | None:intergenic | 30.0% | |
| GATGTGGTTAATGATCTTAA+GGG | + | chr7.2:6962892-6962911 | MS.gene23031:CDS | 30.0% | |
| TGATGTGGTTAATGATCTTA+AGG | + | chr7.2:6962891-6962910 | MS.gene23031:CDS | 30.0% | |
| TTAACCACATCATATGGAAT+TGG | - | chr7.2:6962883-6962902 | None:intergenic | 30.0% | |
| ! | AGGGTTTATGATTTTCTATG+CGG | + | chr7.2:6962197-6962216 | MS.gene23031:intron | 30.0% |
| !! | TTGCTATTTGTGGTTATTTG+TGG | + | chr7.2:6962639-6962658 | MS.gene23031:intron | 30.0% |
| !!! | ATTTCCGAATTTTAGTTCTG+TGG | + | chr7.2:6962090-6962109 | MS.gene23031:intron | 30.0% |
| CATTTGCAAGTGAGTTTATC+CGG | + | chr7.2:6962581-6962600 | MS.gene23031:intron | 35.0% | |
| TACTCCAATTCCATATGATG+TGG | + | chr7.2:6962876-6962895 | MS.gene23031:CDS | 35.0% | |
| TATGTCGATATCAGGGTTAA+CGG | + | chr7.2:6961946-6961965 | MS.gene23031:CDS | 35.0% | |
| TTTGTGGTTATTTGTGGTTC+AGG | + | chr7.2:6962645-6962664 | MS.gene23031:intron | 35.0% | |
| AAACCTGGTTTCATGGTTGA+AGG | + | chr7.2:6962835-6962854 | MS.gene23031:CDS | 40.0% | |
| AAATCATGCAGAGCAAGAAC+CGG | - | chr7.2:6962603-6962622 | None:intergenic | 40.0% | |
| AACGTTACCAAACGTGCTAT+TGG | + | chr7.2:6962027-6962046 | MS.gene23031:CDS | 40.0% | |
| AGGTTAGGAACAAGATTCTC+AGG | + | chr7.2:6962665-6962684 | MS.gene23031:intron | 40.0% | |
| ATTGTACCCTTCTTCCTGAA+TGG | - | chr7.2:6961886-6961905 | None:intergenic | 40.0% | |
| CAAATGGATGCTTAATGCAC+AGG | - | chr7.2:6962527-6962546 | None:intergenic | 40.0% | |
| GAATGATCAACTTAAGGCTG+AGG | + | chr7.2:6962762-6962781 | MS.gene23031:CDS | 40.0% | |
| GTGGTTAATGATCTTAAGGG+AGG | + | chr7.2:6962895-6962914 | MS.gene23031:CDS | 40.0% | |
| TACCTCAGAACCTATCACAT+CGG | + | chr7.2:6961919-6961938 | MS.gene23031:CDS | 40.0% | |
| TCAACCATGAAACCAGGTTT+AGG | - | chr7.2:6962835-6962854 | None:intergenic | 40.0% | |
| ! | GGTTATTTGTGGTTCAGGTT+AGG | + | chr7.2:6962650-6962669 | MS.gene23031:intron | 40.0% |
| AAACCAGGTTTAGGTCCTTC+TGG | - | chr7.2:6962826-6962845 | None:intergenic | 45.0% | |
| ACTCTTTCTCTCGTCCATTC+AGG | + | chr7.2:6961869-6961888 | MS.gene23031:CDS | 45.0% | |
| AGGACCTAAACCTGGTTTCA+TGG | + | chr7.2:6962828-6962847 | MS.gene23031:CDS | 45.0% | |
| ATGCCACACAAGTTCTACCA+TGG | + | chr7.2:6961985-6962004 | MS.gene23031:CDS | 45.0% | |
| CATGGTTGAAGGAGCTACAT+TGG | + | chr7.2:6962846-6962865 | MS.gene23031:CDS | 45.0% | |
| CGGCGATTATGTCGATATCA+GGG | + | chr7.2:6961939-6961958 | MS.gene23031:CDS | 45.0% | |
| CTCAACACCAATAGCACGTT+TGG | - | chr7.2:6962037-6962056 | None:intergenic | 45.0% | |
| GAAGAGAATCCATGTTCGAG+TGG | + | chr7.2:6962687-6962706 | MS.gene23031:CDS | 45.0% | |
| GACATAATCGCCGATGTGAT+AGG | - | chr7.2:6961932-6961951 | None:intergenic | 45.0% | |
| GTGTGGCATACCCTTATGAA+CGG | - | chr7.2:6961974-6961993 | None:intergenic | 45.0% | |
| TAACATGCTCCACTCGAACA+TGG | - | chr7.2:6962699-6962718 | None:intergenic | 45.0% | |
| TCAACTTAAGGCTGAGGCTA+AGG | + | chr7.2:6962768-6962787 | MS.gene23031:CDS | 45.0% | |
| TCGGCGATTATGTCGATATC+AGG | + | chr7.2:6961938-6961957 | MS.gene23031:CDS | 45.0% | |
| TCTCGTCCATTCAGGAAGAA+GGG | + | chr7.2:6961877-6961896 | MS.gene23031:CDS | 45.0% | |
| TGCCACACAAGTTCTACCAT+GGG | + | chr7.2:6961986-6962005 | MS.gene23031:CDS | 45.0% | |
| TGGAGCATGTTATGCCATCT+AGG | + | chr7.2:6962707-6962726 | MS.gene23031:CDS | 45.0% | |
| !! | CAAACGTGCTATTGGTGTTG+AGG | + | chr7.2:6962035-6962054 | MS.gene23031:CDS | 45.0% |
| !! | GTGATAGGTTCTGAGGTAAG+TGG | - | chr7.2:6961917-6961936 | None:intergenic | 45.0% |
| !! | GTTAACGGTGCCGTTCATAA+GGG | + | chr7.2:6961961-6961980 | MS.gene23031:CDS | 45.0% |
| AAGCCAGAAGGACCTAAACC+TGG | + | chr7.2:6962820-6962839 | MS.gene23031:CDS | 50.0% | |
| AAGGCTGAGGCTAAGGCAAA+GGG | + | chr7.2:6962775-6962794 | MS.gene23031:CDS | 50.0% | |
| AAGTTCTACCATGGGCGTAC+CGG | + | chr7.2:6961994-6962013 | MS.gene23031:CDS | 50.0% | |
| CTCTCGTCCATTCAGGAAGA+AGG | + | chr7.2:6961876-6961895 | MS.gene23031:CDS | 50.0% | |
| CTTCCTCAAACGGAACTCCT+CGG | - | chr7.2:6962737-6962756 | None:intergenic | 50.0% | |
| GATCTCAAACCATGACCAGC+CGG | - | chr7.2:6961838-6961857 | None:intergenic | 50.0% | |
| GCTCCTTCAACCATGAAACC+AGG | - | chr7.2:6962841-6962860 | None:intergenic | 50.0% | |
| TAAGGCTGAGGCTAAGGCAA+AGG | + | chr7.2:6962774-6962793 | MS.gene23031:CDS | 50.0% | |
| !! | GGTTAACGGTGCCGTTCATA+AGG | + | chr7.2:6961960-6961979 | MS.gene23031:CDS | 50.0% |
| CGCCCATGGTAGAACTTGTG+TGG | - | chr7.2:6961991-6962010 | None:intergenic | 55.0% | |
| CGCCGATGTGATAGGTTCTG+AGG | - | chr7.2:6961924-6961943 | None:intergenic | 55.0% | |
| GGTAACGTTCCAGACACGAC+CGG | - | chr7.2:6962016-6962035 | None:intergenic | 55.0% | |
| TATGCCATCTAGGTGCACCG+AGG | + | chr7.2:6962717-6962736 | MS.gene23031:CDS | 55.0% | |
| ! | AACTCCTCGGTGCACCTAGA+TGG | - | chr7.2:6962724-6962743 | None:intergenic | 55.0% |
| !! | AGGTTCTGAGGTAAGTGGCG+AGG | - | chr7.2:6961912-6961931 | None:intergenic | 55.0% |
| !! | GGTTCTGAGGTAAGTGGCGA+GGG | - | chr7.2:6961911-6961930 | None:intergenic | 55.0% |
| AGCACCAAGCGCAAGCCAGA+AGG | + | chr7.2:6962808-6962827 | MS.gene23031:CDS | 60.0% | |
| GCACCGAGGAGTTCCGTTTG+AGG | + | chr7.2:6962731-6962750 | MS.gene23031:CDS | 60.0% | |
| !! | AGGTCCTTCTGGCTTGCGCT+TGG | - | chr7.2:6962815-6962834 | None:intergenic | 60.0% |
| AGACACGACCGGTACGCCCA+TGG | - | chr7.2:6962005-6962024 | None:intergenic | 65.0% | |
| ! | ATGGGCGTACCGGTCGTGTC+TGG | + | chr7.2:6962004-6962023 | MS.gene23031:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 6961832 | 6962924 | 6961832 | ID=MS.gene23031 |
| chr7.2 | mRNA | 6961832 | 6962924 | 6961832 | ID=MS.gene23031.t1;Parent=MS.gene23031 |
| chr7.2 | exon | 6961832 | 6962068 | 6961832 | ID=MS.gene23031.t1.exon1;Parent=MS.gene23031.t1 |
| chr7.2 | CDS | 6961832 | 6962068 | 6961832 | ID=cds.MS.gene23031.t1;Parent=MS.gene23031.t1 |
| chr7.2 | exon | 6962667 | 6962924 | 6962667 | ID=MS.gene23031.t1.exon2;Parent=MS.gene23031.t1 |
| chr7.2 | CDS | 6962667 | 6962924 | 6962667 | ID=cds.MS.gene23031.t1;Parent=MS.gene23031.t1 |
| Gene Sequence |
| Protein sequence |
