Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00710.t1 | XP_003629616.1 | 92.6 | 94 | 6 | 1 | 1 | 93 | 16 | 109 | 5.70E-39 | 169.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00710.t1 | O23262 | 41.8 | 98 | 47 | 4 | 2 | 93 | 14 | 107 | 2.8e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00710.t1 | B7FMN1 | 92.6 | 94 | 6 | 1 | 1 | 93 | 16 | 109 | 4.1e-39 | 169.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene00710 | MS.gene035341 | PPI |
| MS.gene041566 | MS.gene00710 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00710.t1 | MTR_8g083150 | 92.553 | 94 | 6 | 1 | 1 | 93 | 16 | 109 | 2.98e-59 | 177 |
| MS.gene00710.t1 | MTR_8g083150 | 92.553 | 94 | 6 | 1 | 1 | 93 | 16 | 109 | 2.99e-59 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00710.t1 | AT4G14010 | 41.837 | 98 | 47 | 4 | 2 | 93 | 14 | 107 | 7.22e-17 | 70.1 |
Find 16 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAGAAGATGAACAATTGA+TGG | 0.375070 | 2.3:-63973571 | MS.gene00710:CDS |
| CCTCTATTATTAGAATTTGA+AGG | 0.422845 | 2.3:+63973414 | None:intergenic |
| TTCTTGATTGCATTCAGCTA+TGG | 0.463317 | 2.3:+63973587 | None:intergenic |
| CCTGCACCACCATTACAAAC+TGG | 0.481857 | 2.3:+63973477 | None:intergenic |
| GAAGCTTATAGTAAATCTGC+AGG | 0.482571 | 2.3:-63973450 | MS.gene00710:CDS |
| CCTTCAAATTCTAATAATAG+AGG | 0.488310 | 2.3:-63973414 | MS.gene00710:CDS |
| CCAGTTTGTAATGGTGGTGC+AGG | 0.504468 | 2.3:-63973477 | MS.gene00710:CDS |
| TTATTAGAATTTGAAGGTGG+AGG | 0.509265 | 2.3:+63973420 | None:intergenic |
| AGAGATGAACCAGTTTGTAA+TGG | 0.566659 | 2.3:-63973486 | MS.gene00710:CDS |
| CAAAAGAGATACATTTCTGA+AGG | 0.578676 | 2.3:-63973519 | MS.gene00710:CDS |
| CTATTATTAGAATTTGAAGG+TGG | 0.607589 | 2.3:+63973417 | None:intergenic |
| GATGAACCAGTTTGTAATGG+TGG | 0.609246 | 2.3:-63973483 | MS.gene00710:CDS |
| CATCATGCAAGCATATGCAA+TGG | 0.634173 | 2.3:-63973612 | MS.gene00710:CDS |
| TGGAGTCTGAAATAAGCAGA+AGG | 0.635317 | 2.3:-63973551 | MS.gene00710:CDS |
| GTTTGTAATGGTGGTGCAGG+TGG | 0.645633 | 2.3:-63973474 | MS.gene00710:CDS |
| AAGCTTATAGTAAATCTGCA+GGG | 0.695265 | 2.3:-63973449 | MS.gene00710:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CCTCTATTATTAGAATTTGA+AGG | + | chr2.3:63973662-63973681 | None:intergenic | 25.0% |
| ! | CCTTCAAATTCTAATAATAG+AGG | - | chr2.3:63973659-63973678 | MS.gene00710:CDS | 25.0% |
| ! | CTATTATTAGAATTTGAAGG+TGG | + | chr2.3:63973659-63973678 | None:intergenic | 25.0% |
| AAGCTTATAGTAAATCTGCA+GGG | - | chr2.3:63973624-63973643 | MS.gene00710:CDS | 30.0% | |
| CAAAAGAGATACATTTCTGA+AGG | - | chr2.3:63973554-63973573 | MS.gene00710:CDS | 30.0% | |
| TCAAGAAGATGAACAATTGA+TGG | - | chr2.3:63973502-63973521 | MS.gene00710:CDS | 30.0% | |
| TTATTAGAATTTGAAGGTGG+AGG | + | chr2.3:63973656-63973675 | None:intergenic | 30.0% | |
| ! | TGAAATAAGCAGAAGGTTTT+TGG | - | chr2.3:63973529-63973548 | MS.gene00710:CDS | 30.0% |
| AGAGATGAACCAGTTTGTAA+TGG | - | chr2.3:63973587-63973606 | MS.gene00710:CDS | 35.0% | |
| GAAGCTTATAGTAAATCTGC+AGG | - | chr2.3:63973623-63973642 | MS.gene00710:CDS | 35.0% | |
| TTCTTGATTGCATTCAGCTA+TGG | + | chr2.3:63973489-63973508 | None:intergenic | 35.0% | |
| CATCATGCAAGCATATGCAA+TGG | - | chr2.3:63973461-63973480 | MS.gene00710:CDS | 40.0% | |
| GATGAACCAGTTTGTAATGG+TGG | - | chr2.3:63973590-63973609 | MS.gene00710:CDS | 40.0% | |
| ! | TGGAGTCTGAAATAAGCAGA+AGG | - | chr2.3:63973522-63973541 | MS.gene00710:CDS | 40.0% |
| CCTGCACCACCATTACAAAC+TGG | + | chr2.3:63973599-63973618 | None:intergenic | 50.0% | |
| ! | CCAGTTTGTAATGGTGGTGC+AGG | - | chr2.3:63973596-63973615 | MS.gene00710:CDS | 50.0% |
| ! | GTTTGTAATGGTGGTGCAGG+TGG | - | chr2.3:63973599-63973618 | MS.gene00710:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 63973407 | 63973688 | 63973407 | ID=MS.gene00710 |
| chr2.3 | mRNA | 63973407 | 63973688 | 63973407 | ID=MS.gene00710.t1;Parent=MS.gene00710 |
| chr2.3 | exon | 63973407 | 63973688 | 63973407 | ID=MS.gene00710.t1.exon1;Parent=MS.gene00710.t1 |
| chr2.3 | CDS | 63973407 | 63973688 | 63973407 | ID=cds.MS.gene00710.t1;Parent=MS.gene00710.t1 |
| Gene Sequence |
| Protein sequence |
