AlfalfaGEDB | MS.gene041566

M. sativa cultivar XinJiangDaYe / MS.gene041566

KEGG: -
GO: -
PFAM: PF05498.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041566.t1	XP_003609670.2	96.8	126	4	0	1	126	1	126	3.20E-61	244.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041566.t1	Q9LUS7	60.8	130	31	3	11	126	14	137	6.7e-31	134.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041566.t1	G7JLH2	96.8	126	4	0	1	126	1	126	2.3e-61	244.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene041566	MS.gene01006	PPI
MS.gene08826	MS.gene041566	PPI
MS.gene041566	MS.gene023479	PPI
MS.gene041566	MS.gene01008	PPI
MS.gene00716	MS.gene041566	PPI
MS.gene041566	MS.gene00713	PPI
MS.gene041566	MS.gene00710	PPI
MS.gene041566	MS.gene09266	PPI
MS.gene041566	MS.gene08980	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041566.t1	MTR_4g119860	96.825	126	4	0	1	126	1	126	1.47e-86	248
MS.gene041566.t1	MTR_7g113080	57.025	121	45	2	9	125	9	126	1.91e-41	134
MS.gene041566.t1	MTR_7g108600	50.820	122	46	3	9	125	4	116	3.67e-32	110
MS.gene041566.t1	MTR_4g088685	45.082	122	43	3	9	126	8	109	3.50e-28	99.8
MS.gene041566.t1	MTR_3g084350	42.063	126	57	6	10	126	10	128	7.61e-23	87.0
MS.gene041566.t1	MTR_6g060260	40.678	118	47	4	11	126	7	103	6.30e-22	84.0
MS.gene041566.t1	MTR_8g083150	37.500	120	54	5	10	125	14	116	9.80e-14	63.2
MS.gene041566.t1	MTR_8g083150	37.500	120	54	5	10	125	14	116	1.08e-13	63.2
MS.gene041566.t1	MTR_5g017160	48.101	79	34	3	55	126	50	128	5.46e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041566.t1	AT4G15800	62.609	115	34	2	12	126	11	116	1.51e-43	139
MS.gene041566.t1	AT3G16570	57.576	132	36	3	9	126	12	137	2.64e-40	131
MS.gene041566.t1	AT3G16570	57.576	132	36	3	9	126	12	137	2.64e-40	131
MS.gene041566.t1	AT3G16570	57.576	132	36	3	9	126	12	137	2.64e-40	131
MS.gene041566.t1	AT1G02900	51.667	120	49	3	9	125	6	119	3.61e-34	115
MS.gene041566.t1	AT3G05490	54.331	127	44	3	5	126	2	119	4.09e-31	107
MS.gene041566.t1	AT2G33775	68.116	69	21	1	59	126	39	107	7.98e-28	99.0
MS.gene041566.t1	AT1G28270	46.218	119	46	4	10	126	5	107	1.21e-25	93.6
MS.gene041566.t1	AT4G13950	41.525	118	49	5	11	126	7	106	8.50e-20	78.6
MS.gene041566.t1	AT3G23805	62.069	58	20	2	70	126	55	111	1.94e-16	70.1
MS.gene041566.t1	AT4G14010	37.209	129	57	7	2	126	7	115	5.20e-13	61.2

Find 40 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGATGGATTCTGAGAGCAAC+AGG	0.416498	8.2:-10774777	MS.gene041566:CDS
TAATTGTCGGCCTGGTGCTC+AGG	0.420371	8.2:-10774668	MS.gene041566:CDS
GTGCTTCTTATTATAATTGT+CGG	0.423020	8.2:-10774681	MS.gene041566:CDS
CTCAGTTTCATCTTCATCTC+CGG	0.447217	8.2:+10774804	None:intergenic
GGAGTCCGACGGGAGGAAGA+AGG	0.482366	8.2:+10774864	None:intergenic
CCGTCGGACTCCGGCTGCCG+TGG	0.488036	8.2:-10774853	MS.gene041566:CDS
TTCTTCCTCCCGTCGGACTC+CGG	0.491619	8.2:-10774862	MS.gene041566:CDS
GGAGGAAGAAGGAGCTGAGC+TGG	0.494801	8.2:+10774875	None:intergenic
TGCGATGGATCCACGGCAGC+CGG	0.495967	8.2:+10774843	None:intergenic
TCTTATTATAATTGTCGGCC+TGG	0.507188	8.2:-10774676	MS.gene041566:CDS
GCAGTGCCATCACTCGATGC+AGG	0.524333	8.2:-10774624	MS.gene041566:CDS
AGATGAAACTGAGTTTATGA+TGG	0.528256	8.2:-10774794	MS.gene041566:CDS
GAAGGAGCTGAGCTGGTTGT+CGG	0.531077	8.2:+10774882	None:intergenic
TAAGGATTAGCCTGAGCACC+AGG	0.532157	8.2:+10774658	None:intergenic
CACGGCAGCCGGAGTCCGAC+GGG	0.532407	8.2:+10774854	None:intergenic
GCTGGTTGTCGGTGAAATCA+AGG	0.536840	8.2:+10774893	None:intergenic
GAGTGATGGCACTGCATCCA+CGG	0.542603	8.2:+10774632	None:intergenic
GCACTGCATCCACGGCGGTA+AGG	0.551017	8.2:+10774640	None:intergenic
GCAAGAAGGTATATTAGCTA+TGG	0.559047	8.2:-10774742	MS.gene041566:CDS
GTCGGTGAAATCAAGGGTGG+AGG	0.561416	8.2:+10774900	None:intergenic
CAGCTCCTTCTTCCTCCCGT+CGG	0.563547	8.2:-10774869	MS.gene041566:CDS
TTAGCTATGGTGCGCTGAGG+AGG	0.572142	8.2:-10774729	MS.gene041566:CDS
CCACGGCAGCCGGAGTCCGA+CGG	0.574419	8.2:+10774853	None:intergenic
TCAACGCCTGCATCGAGTGA+TGG	0.583534	8.2:+10774618	None:intergenic
TGCACTCTGCGATGGATCCA+CGG	0.587914	8.2:+10774836	None:intergenic
GAAGCACCACGTCTTGAGCA+AGG	0.588288	8.2:+10774697	None:intergenic
GAGAAGACTGCACTCTGCGA+TGG	0.593105	8.2:+10774828	None:intergenic
AAGCACCACGTCTTGAGCAA+GGG	0.613382	8.2:+10774698	None:intergenic
ATGATGAAAGTGACACGTGG+AGG	0.620764	8.2:+10774923	None:intergenic
TGATGAAAGTGACACGTGGA+GGG	0.626032	8.2:+10774924	None:intergenic
CAGGCTAATCCTTACCGCCG+TGG	0.626471	8.2:-10774649	MS.gene041566:CDS
GGCAGCCGGAGTCCGACGGG+AGG	0.633730	8.2:+10774857	None:intergenic
GCAGAGTGCAGTCTTCTCGC+CGG	0.644784	8.2:-10774823	MS.gene041566:CDS
CTGGTTGTCGGTGAAATCAA+GGG	0.650378	8.2:+10774894	None:intergenic
ACTGTCCCTTGCTCAAGACG+TGG	0.665357	8.2:-10774703	MS.gene041566:CDS
GTTGTCGGTGAAATCAAGGG+TGG	0.683736	8.2:+10774897	None:intergenic
ATATTAGCTATGGTGCGCTG+AGG	0.691098	8.2:-10774732	MS.gene041566:CDS
AGAAAAGAAAGAAAACACAC+AGG	0.720832	8.2:+10774959	None:intergenic
AGGATGATGAAAGTGACACG+TGG	0.737888	8.2:+10774920	None:intergenic
TGATGGCACTGCATCCACGG+CGG	0.766443	8.2:+10774635	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTGCTTCTTATTATAATTGT+CGG	-	chr8.2:10774913-10774932	MS.gene041566:CDS	25.0%
	AGAAAAGAAAGAAAACACAC+AGG	+	chr8.2:10774638-10774657	None:intergenic	30.0%
	AGATGAAACTGAGTTTATGA+TGG	-	chr8.2:10774800-10774819	MS.gene041566:CDS	30.0%
	TCTTATTATAATTGTCGGCC+TGG	-	chr8.2:10774918-10774937	MS.gene041566:CDS	35.0%
!	GCAAGAAGGTATATTAGCTA+TGG	-	chr8.2:10774852-10774871	MS.gene041566:CDS	35.0%
	CTCAGTTTCATCTTCATCTC+CGG	+	chr8.2:10774793-10774812	None:intergenic	40.0%
	AGGATGATGAAAGTGACACG+TGG	+	chr8.2:10774677-10774696	None:intergenic	45.0%
	ATATTAGCTATGGTGCGCTG+AGG	-	chr8.2:10774862-10774881	MS.gene041566:CDS	45.0%
	ATGATGAAAGTGACACGTGG+AGG	+	chr8.2:10774674-10774693	None:intergenic	45.0%
	CTGGTTGTCGGTGAAATCAA+GGG	+	chr8.2:10774703-10774722	None:intergenic	45.0%
	TGATGAAAGTGACACGTGGA+GGG	+	chr8.2:10774673-10774692	None:intergenic	45.0%
!!	TGATGGATTCTGAGAGCAAC+AGG	-	chr8.2:10774817-10774836	MS.gene041566:CDS	45.0%
	AAGCACCACGTCTTGAGCAA+GGG	+	chr8.2:10774899-10774918	None:intergenic	50.0%
	GCTGGTTGTCGGTGAAATCA+AGG	+	chr8.2:10774704-10774723	None:intergenic	50.0%
	GTTGTCGGTGAAATCAAGGG+TGG	+	chr8.2:10774700-10774719	None:intergenic	50.0%
!!	GGCGCATTTTAGCAGCAAGA+AGG	-	chr8.2:10774838-10774857	MS.gene041566:CDS	50.0%
!!	TAAGGATTAGCCTGAGCACC+AGG	+	chr8.2:10774939-10774958	None:intergenic	50.0%
	ACTGTCCCTTGCTCAAGACG+TGG	-	chr8.2:10774891-10774910	MS.gene041566:CDS	55.0%
	GAAGGAGCTGAGCTGGTTGT+CGG	+	chr8.2:10774715-10774734	None:intergenic	55.0%
	GAGAAGACTGCACTCTGCGA+TGG	+	chr8.2:10774769-10774788	None:intergenic	55.0%
	GAGTGATGGCACTGCATCCA+CGG	+	chr8.2:10774965-10774984	None:intergenic	55.0%
	GTCGGTGAAATCAAGGGTGG+AGG	+	chr8.2:10774697-10774716	None:intergenic	55.0%
	TAATTGTCGGCCTGGTGCTC+AGG	-	chr8.2:10774926-10774945	MS.gene041566:CDS	55.0%
	TCAACGCCTGCATCGAGTGA+TGG	+	chr8.2:10774979-10774998	None:intergenic	55.0%
	TGCACTCTGCGATGGATCCA+CGG	+	chr8.2:10774761-10774780	None:intergenic	55.0%
	TTAGCTATGGTGCGCTGAGG+AGG	-	chr8.2:10774865-10774884	MS.gene041566:CDS	55.0%
!	GAAGCACCACGTCTTGAGCA+AGG	+	chr8.2:10774900-10774919	None:intergenic	55.0%
	CAGCTCCTTCTTCCTCCCGT+CGG	-	chr8.2:10774725-10774744	MS.gene041566:CDS	60.0%
	CAGGCTAATCCTTACCGCCG+TGG	-	chr8.2:10774945-10774964	MS.gene041566:CDS	60.0%
	GCAGAGTGCAGTCTTCTCGC+CGG	-	chr8.2:10774771-10774790	MS.gene041566:CDS	60.0%
	GCAGTGCCATCACTCGATGC+AGG	-	chr8.2:10774970-10774989	MS.gene041566:CDS	60.0%
	GGAGGAAGAAGGAGCTGAGC+TGG	+	chr8.2:10774722-10774741	None:intergenic	60.0%
	TGATGGCACTGCATCCACGG+CGG	+	chr8.2:10774962-10774981	None:intergenic	60.0%
	TTCTTCCTCCCGTCGGACTC+CGG	-	chr8.2:10774732-10774751	MS.gene041566:CDS	60.0%
	GCACTGCATCCACGGCGGTA+AGG	+	chr8.2:10774957-10774976	None:intergenic	65.0%
	GGAGTCCGACGGGAGGAAGA+AGG	+	chr8.2:10774733-10774752	None:intergenic	65.0%
	TGCGATGGATCCACGGCAGC+CGG	+	chr8.2:10774754-10774773	None:intergenic	65.0%
	CACGGCAGCCGGAGTCCGAC+GGG	+	chr8.2:10774743-10774762	None:intergenic	75.0%
	CCACGGCAGCCGGAGTCCGA+CGG	+	chr8.2:10774744-10774763	None:intergenic	75.0%
!	CCGTCGGACTCCGGCTGCCG+TGG	-	chr8.2:10774741-10774760	MS.gene041566:CDS	80.0%
!	GGCAGCCGGAGTCCGACGGG+AGG	+	chr8.2:10774740-10774759	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	10774618	10774998	10774618	ID=MS.gene041566
chr8.2	mRNA	10774618	10774998	10774618	ID=MS.gene041566.t1;Parent=MS.gene041566
chr8.2	exon	10774618	10774998	10774618	ID=MS.gene041566.t1.exon1;Parent=MS.gene041566.t1
chr8.2	CDS	10774618	10774998	10774618	ID=cds.MS.gene041566.t1;Parent=MS.gene041566.t1

Gene Sequence

>MS.gene041566

ATGAGAACGAGAAACAACCTGTGTGTTTTCTTTCTTTTCTTTTCAATCTTAGCCCTCCACGTGTCACTTTCATCATCCTCCACCCTTGATTTCACCGACAACCAGCTCAGCTCCTTCTTCCTCCCGTCGGACTCCGGCTGCCGTGGATCCATCGCAGAGTGCAGTCTTCTCGCCGGAGATGAAGATGAAACTGAGTTTATGATGGATTCTGAGAGCAACAGGCGCATTTTAGCAGCAAGAAGGTATATTAGCTATGGTGCGCTGAGGAGGAACACTGTCCCTTGCTCAAGACGTGGTGCTTCTTATTATAATTGTCGGCCTGGTGCTCAGGCTAATCCTTACCGCCGTGGATGCAGTGCCATCACTCGATGCAGGCGTTGA

Protein sequence

>MS.gene041566.t1

MRTRNNLCVFFLFFSILALHVSLSSSSTLDFTDNQLSSFFLPSDSGCRGSIAECSLLAGDEDETEFMMDSESNRRILAARRYISYGALRRNTVPCSRRGASYYNCRPGAQANPYRRGCSAITRCRR