Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035341.t1 | XP_003609670.2 | 96.8 | 126 | 4 | 0 | 1 | 126 | 1 | 126 | 3.20E-61 | 244.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035341.t1 | Q9LUS7 | 60.8 | 130 | 31 | 3 | 11 | 126 | 14 | 137 | 6.7e-31 | 134.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035341.t1 | G7JLH2 | 96.8 | 126 | 4 | 0 | 1 | 126 | 1 | 126 | 2.3e-61 | 244.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene00710 | MS.gene035341 | PPI |
| MS.gene08980 | MS.gene035341 | PPI |
| MS.gene08826 | MS.gene035341 | PPI |
| MS.gene00713 | MS.gene035341 | PPI |
| MS.gene01008 | MS.gene035341 | PPI |
| MS.gene01006 | MS.gene035341 | PPI |
| MS.gene023479 | MS.gene035341 | PPI |
| MS.gene09266 | MS.gene035341 | PPI |
| MS.gene00716 | MS.gene035341 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035341.t1 | MTR_4g119860 | 96.825 | 126 | 4 | 0 | 1 | 126 | 1 | 126 | 1.47e-86 | 248 |
| MS.gene035341.t1 | MTR_7g113080 | 57.025 | 121 | 45 | 2 | 9 | 125 | 9 | 126 | 1.91e-41 | 134 |
| MS.gene035341.t1 | MTR_7g108600 | 50.820 | 122 | 46 | 3 | 9 | 125 | 4 | 116 | 3.67e-32 | 110 |
| MS.gene035341.t1 | MTR_4g088685 | 45.082 | 122 | 43 | 3 | 9 | 126 | 8 | 109 | 3.50e-28 | 99.8 |
| MS.gene035341.t1 | MTR_3g084350 | 42.063 | 126 | 57 | 6 | 10 | 126 | 10 | 128 | 7.61e-23 | 87.0 |
| MS.gene035341.t1 | MTR_6g060260 | 40.678 | 118 | 47 | 4 | 11 | 126 | 7 | 103 | 6.30e-22 | 84.0 |
| MS.gene035341.t1 | MTR_8g083150 | 37.500 | 120 | 54 | 5 | 10 | 125 | 14 | 116 | 9.80e-14 | 63.2 |
| MS.gene035341.t1 | MTR_8g083150 | 37.500 | 120 | 54 | 5 | 10 | 125 | 14 | 116 | 1.08e-13 | 63.2 |
| MS.gene035341.t1 | MTR_5g017160 | 48.101 | 79 | 34 | 3 | 55 | 126 | 50 | 128 | 5.46e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035341.t1 | AT4G15800 | 62.609 | 115 | 34 | 2 | 12 | 126 | 11 | 116 | 1.51e-43 | 139 |
| MS.gene035341.t1 | AT3G16570 | 57.576 | 132 | 36 | 3 | 9 | 126 | 12 | 137 | 2.64e-40 | 131 |
| MS.gene035341.t1 | AT3G16570 | 57.576 | 132 | 36 | 3 | 9 | 126 | 12 | 137 | 2.64e-40 | 131 |
| MS.gene035341.t1 | AT3G16570 | 57.576 | 132 | 36 | 3 | 9 | 126 | 12 | 137 | 2.64e-40 | 131 |
| MS.gene035341.t1 | AT1G02900 | 51.667 | 120 | 49 | 3 | 9 | 125 | 6 | 119 | 3.61e-34 | 115 |
| MS.gene035341.t1 | AT3G05490 | 54.331 | 127 | 44 | 3 | 5 | 126 | 2 | 119 | 4.09e-31 | 107 |
| MS.gene035341.t1 | AT2G33775 | 68.116 | 69 | 21 | 1 | 59 | 126 | 39 | 107 | 7.98e-28 | 99.0 |
| MS.gene035341.t1 | AT1G28270 | 46.218 | 119 | 46 | 4 | 10 | 126 | 5 | 107 | 1.21e-25 | 93.6 |
| MS.gene035341.t1 | AT4G13950 | 41.525 | 118 | 49 | 5 | 11 | 126 | 7 | 106 | 8.50e-20 | 78.6 |
| MS.gene035341.t1 | AT3G23805 | 62.069 | 58 | 20 | 2 | 70 | 126 | 55 | 111 | 1.94e-16 | 70.1 |
| MS.gene035341.t1 | AT4G14010 | 37.209 | 129 | 57 | 7 | 2 | 126 | 7 | 115 | 5.20e-13 | 61.2 |
Find 0 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTGCTTCTTATTATAATTGT+CGG | + | 32602:60531-60550 | MS.gene035341:CDS | 25.0% |
| AGAAAAGAAAGAAAACACAG+AGG | - | 32602:60256-60275 | None:intergenic | 30.0% | |
| AGATGAAACCGAGTTTATGA+TGG | + | 32602:60418-60437 | MS.gene035341:CDS | 35.0% | |
| CTCAGAATCCATCATAAACT+CGG | - | 32602:60429-60448 | None:intergenic | 35.0% | |
| TCTTATTATAATTGTCGGCC+TGG | + | 32602:60536-60555 | MS.gene035341:CDS | 35.0% | |
| ! | GCAAGAAGGTATATTAGCTA+TGG | + | 32602:60470-60489 | MS.gene035341:CDS | 35.0% |
| AGGATGATGAAAGTGACACG+TGG | - | 32602:60295-60314 | None:intergenic | 45.0% | |
| ATATTAGCTATGGTGCGCTG+AGG | + | 32602:60480-60499 | MS.gene035341:CDS | 45.0% | |
| ATGATGAAAGTGACACGTGG+AGG | - | 32602:60292-60311 | None:intergenic | 45.0% | |
| CTCGGTTTCATCTTCATCTC+CGG | - | 32602:60411-60430 | None:intergenic | 45.0% | |
| CTGGTTGTCGGTGAAATCAA+GGG | - | 32602:60321-60340 | None:intergenic | 45.0% | |
| TGATGAAAGTGACACGTGGA+GGG | - | 32602:60291-60310 | None:intergenic | 45.0% | |
| !! | TGATGGATTCTGAGAGCAAC+AGG | + | 32602:60435-60454 | MS.gene035341:CDS | 45.0% |
| AAGCACCACGTCTTGAGCAA+GGG | - | 32602:60517-60536 | None:intergenic | 50.0% | |
| GCTGGTTGTCGGTGAAATCA+AGG | - | 32602:60322-60341 | None:intergenic | 50.0% | |
| GTTGTCGGTGAAATCAAGGG+TGG | - | 32602:60318-60337 | None:intergenic | 50.0% | |
| !! | GGCGCATTTTAGCAGCAAGA+AGG | + | 32602:60456-60475 | MS.gene035341:CDS | 50.0% |
| !! | TAAGGATTAGCCTGAGCACC+AGG | - | 32602:60557-60576 | None:intergenic | 50.0% |
| ACTGTCCCTTGCTCAAGACG+TGG | + | 32602:60509-60528 | MS.gene035341:CDS | 55.0% | |
| GAAGGAGCTGAGCTGGTTGT+CGG | - | 32602:60333-60352 | None:intergenic | 55.0% | |
| GAGAAGACTGCACTCTGCGA+TGG | - | 32602:60387-60406 | None:intergenic | 55.0% | |
| GAGTGATGGCACTGCATCCA+CGG | - | 32602:60583-60602 | None:intergenic | 55.0% | |
| GTCGGTGAAATCAAGGGTGG+AGG | - | 32602:60315-60334 | None:intergenic | 55.0% | |
| TAATTGTCGGCCTGGTGCTC+AGG | + | 32602:60544-60563 | MS.gene035341:CDS | 55.0% | |
| TCAACGCCTGCATCGAGTGA+TGG | - | 32602:60597-60616 | None:intergenic | 55.0% | |
| TGCACTCTGCGATGGATCCA+CGG | - | 32602:60379-60398 | None:intergenic | 55.0% | |
| TTAGCTATGGTGCGCTGAGG+AGG | + | 32602:60483-60502 | MS.gene035341:CDS | 55.0% | |
| ! | GAAGCACCACGTCTTGAGCA+AGG | - | 32602:60518-60537 | None:intergenic | 55.0% |
| CAGCTCCTTCTTCCTCCCGT+CGG | + | 32602:60343-60362 | MS.gene035341:CDS | 60.0% | |
| CAGGCTAATCCTTACCGCCG+TGG | + | 32602:60563-60582 | MS.gene035341:CDS | 60.0% | |
| GCAGAGTGCAGTCTTCTCGC+CGG | + | 32602:60389-60408 | MS.gene035341:CDS | 60.0% | |
| GCAGTGCCATCACTCGATGC+AGG | + | 32602:60588-60607 | MS.gene035341:CDS | 60.0% | |
| GGAGGAAGAAGGAGCTGAGC+TGG | - | 32602:60340-60359 | None:intergenic | 60.0% | |
| TGATGGCACTGCATCCACGG+CGG | - | 32602:60580-60599 | None:intergenic | 60.0% | |
| TTCTTCCTCCCGTCGGACTC+CGG | + | 32602:60350-60369 | MS.gene035341:CDS | 60.0% | |
| GCACTGCATCCACGGCGGTA+AGG | - | 32602:60575-60594 | None:intergenic | 65.0% | |
| GGAGTCCGACGGGAGGAAGA+AGG | - | 32602:60351-60370 | None:intergenic | 65.0% | |
| TGCGATGGATCCACGGCAGC+CGG | - | 32602:60372-60391 | None:intergenic | 65.0% | |
| CACGGCAGCCGGAGTCCGAC+GGG | - | 32602:60361-60380 | None:intergenic | 75.0% | |
| CCACGGCAGCCGGAGTCCGA+CGG | - | 32602:60362-60381 | None:intergenic | 75.0% | |
| ! | CCGTCGGACTCCGGCTGCCG+TGG | + | 32602:60359-60378 | MS.gene035341:CDS | 80.0% |
| ! | GGCAGCCGGAGTCCGACGGG+AGG | - | 32602:60358-60377 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32602 | gene | 60236 | 60616 | 60236 | ID=MS.gene035341 |
| 32602 | mRNA | 60236 | 60616 | 60236 | ID=MS.gene035341.t1;Parent=MS.gene035341 |
| 32602 | exon | 60236 | 60616 | 60236 | ID=MS.gene035341.t1.exon1;Parent=MS.gene035341.t1 |
| 32602 | CDS | 60236 | 60616 | 60236 | ID=cds.MS.gene035341.t1;Parent=MS.gene035341.t1 |
| Gene Sequence |
| Protein sequence |
