Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00713.t1 | XP_003629616.1 | 94.7 | 114 | 5 | 1 | 1 | 113 | 1 | 114 | 3.90E-50 | 207.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00713.t1 | O23262 | 43.5 | 108 | 51 | 4 | 12 | 113 | 9 | 112 | 9.6e-13 | 74.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00713.t1 | B7FMN1 | 94.7 | 114 | 5 | 1 | 1 | 113 | 1 | 114 | 2.8e-50 | 207.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene00713 | MS.gene035341 | PPI |
| MS.gene041566 | MS.gene00713 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00713.t1 | MTR_8g083150 | 94.737 | 114 | 5 | 1 | 1 | 113 | 1 | 114 | 3.21e-75 | 218 |
| MS.gene00713.t1 | MTR_8g083150 | 94.737 | 114 | 5 | 1 | 1 | 113 | 1 | 114 | 3.39e-75 | 218 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00713.t1 | AT4G14010 | 43.636 | 110 | 47 | 5 | 10 | 113 | 12 | 112 | 2.59e-21 | 82.0 |
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAGAAGATGAACAATTGA+TGG | 0.375070 | 2.3:+64012466 | MS.gene00713:CDS |
| CCTCTATTATTAGAATTTGA+AGG | 0.422845 | 2.3:-64012623 | None:intergenic |
| CCTGCACCACCATTACAAAC+TGG | 0.451169 | 2.3:-64012560 | None:intergenic |
| TTCTTGATTGCATTCAGCTA+TGG | 0.463317 | 2.3:-64012450 | None:intergenic |
| GCATGAAGTAGAAGAAGGTA+AGG | 0.469014 | 2.3:-64012352 | None:intergenic |
| GAAGCTTATAGTAAATCTGC+AGG | 0.482571 | 2.3:+64012587 | MS.gene00713:CDS |
| CCTTCAAATTCTAATAATAG+AGG | 0.488310 | 2.3:+64012623 | MS.gene00713:CDS |
| TTATTAGAATTTGAAGGTGG+AGG | 0.509265 | 2.3:-64012617 | None:intergenic |
| CCAGTTTGTAATGGTGGTGC+AGG | 0.525366 | 2.3:+64012560 | MS.gene00713:CDS |
| GATAAACCAGTTTGTAATGG+TGG | 0.534773 | 2.3:+64012554 | MS.gene00713:CDS |
| AGAGATAAACCAGTTTGTAA+TGG | 0.541924 | 2.3:+64012551 | MS.gene00713:CDS |
| GAAGAGCATGAAGTAGAAGA+AGG | 0.567293 | 2.3:-64012357 | None:intergenic |
| CAAAAGAGATACATTTCTGA+AGG | 0.578266 | 2.3:+64012518 | MS.gene00713:CDS |
| CTATTATTAGAATTTGAAGG+TGG | 0.607589 | 2.3:-64012620 | None:intergenic |
| CATCATGCAAGCATATGCAA+TGG | 0.634173 | 2.3:+64012425 | MS.gene00713:CDS |
| TGGAGTCTGAAATAAGCAGA+AGG | 0.635317 | 2.3:+64012486 | MS.gene00713:CDS |
| GTTTGTAATGGTGGTGCAGG+TGG | 0.645633 | 2.3:+64012563 | MS.gene00713:CDS |
| AAGCTTATAGTAAATCTGCA+GGG | 0.695265 | 2.3:+64012588 | MS.gene00713:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CCTCTATTATTAGAATTTGA+AGG | - | chr2.3:64012626-64012645 | None:intergenic | 25.0% |
| ! | CCTTCAAATTCTAATAATAG+AGG | + | chr2.3:64012623-64012642 | MS.gene00713:CDS | 25.0% |
| ! | CTATTATTAGAATTTGAAGG+TGG | - | chr2.3:64012623-64012642 | None:intergenic | 25.0% |
| AAGCTTATAGTAAATCTGCA+GGG | + | chr2.3:64012588-64012607 | MS.gene00713:CDS | 30.0% | |
| AGAGATAAACCAGTTTGTAA+TGG | + | chr2.3:64012551-64012570 | MS.gene00713:CDS | 30.0% | |
| CAAAAGAGATACATTTCTGA+AGG | + | chr2.3:64012518-64012537 | MS.gene00713:CDS | 30.0% | |
| TCAAGAAGATGAACAATTGA+TGG | + | chr2.3:64012466-64012485 | MS.gene00713:CDS | 30.0% | |
| TTATTAGAATTTGAAGGTGG+AGG | - | chr2.3:64012620-64012639 | None:intergenic | 30.0% | |
| ! | TGAAATAAGCAGAAGGTTTT+TGG | + | chr2.3:64012493-64012512 | MS.gene00713:CDS | 30.0% |
| GAAGCTTATAGTAAATCTGC+AGG | + | chr2.3:64012587-64012606 | MS.gene00713:CDS | 35.0% | |
| GATAAACCAGTTTGTAATGG+TGG | + | chr2.3:64012554-64012573 | MS.gene00713:CDS | 35.0% | |
| TTCTTGATTGCATTCAGCTA+TGG | - | chr2.3:64012453-64012472 | None:intergenic | 35.0% | |
| CATCATGCAAGCATATGCAA+TGG | + | chr2.3:64012425-64012444 | MS.gene00713:CDS | 40.0% | |
| GCATGAAGTAGAAGAAGGTA+AGG | - | chr2.3:64012355-64012374 | None:intergenic | 40.0% | |
| ! | GAAGAGCATGAAGTAGAAGA+AGG | - | chr2.3:64012360-64012379 | None:intergenic | 40.0% |
| ! | TGGAGTCTGAAATAAGCAGA+AGG | + | chr2.3:64012486-64012505 | MS.gene00713:CDS | 40.0% |
| CCTGCACCACCATTACAAAC+TGG | - | chr2.3:64012563-64012582 | None:intergenic | 50.0% | |
| ! | CCAGTTTGTAATGGTGGTGC+AGG | + | chr2.3:64012560-64012579 | MS.gene00713:CDS | 50.0% |
| ! | GTTTGTAATGGTGGTGCAGG+TGG | + | chr2.3:64012563-64012582 | MS.gene00713:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 64012326 | 64012665 | 64012326 | ID=MS.gene00713 |
| chr2.3 | mRNA | 64012326 | 64012665 | 64012326 | ID=MS.gene00713.t1;Parent=MS.gene00713 |
| chr2.3 | exon | 64012326 | 64012665 | 64012326 | ID=MS.gene00713.t1.exon1;Parent=MS.gene00713.t1 |
| chr2.3 | CDS | 64012326 | 64012665 | 64012326 | ID=cds.MS.gene00713.t1;Parent=MS.gene00713.t1 |
| Gene Sequence |
| Protein sequence |
