Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00716.t1 | XP_003629616.1 | 95 | 119 | 5 | 1 | 1 | 118 | 1 | 119 | 5.20E-53 | 216.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00716.t1 | O23262 | 44.2 | 113 | 53 | 4 | 12 | 118 | 9 | 117 | 3.1e-14 | 79.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00716.t1 | B7FMN1 | 95.0 | 119 | 5 | 1 | 1 | 118 | 1 | 119 | 3.7e-53 | 216.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene00716 | MS.gene041566 | PPI |
| MS.gene00716 | MS.gene035341 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00716.t1 | MTR_8g083150 | 94.958 | 119 | 5 | 1 | 1 | 118 | 1 | 119 | 3.51e-79 | 228 |
| MS.gene00716.t1 | MTR_8g083150 | 94.828 | 116 | 5 | 1 | 1 | 115 | 1 | 116 | 3.68e-77 | 223 |
| MS.gene00716.t1 | MTR_4g119860 | 35.833 | 120 | 55 | 5 | 14 | 115 | 10 | 125 | 5.70e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00716.t1 | AT4G14010 | 44.348 | 115 | 49 | 5 | 10 | 118 | 12 | 117 | 3.15e-23 | 87.0 |
| MS.gene00716.t1 | AT1G02900 | 36.522 | 115 | 65 | 3 | 6 | 116 | 10 | 120 | 1.47e-12 | 60.1 |
| MS.gene00716.t1 | AT4G15800 | 37.190 | 121 | 64 | 5 | 1 | 115 | 1 | 115 | 3.38e-11 | 56.2 |
Find 19 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCTAAGTATTATCGTTGT+AGG | 0.352031 | 2.3:+64115224 | MS.gene00716:CDS |
| TCAAGAAGATGAACAATTGA+TGG | 0.375070 | 2.3:+64115042 | MS.gene00716:CDS |
| CCTCTATTATTAGAATTTGA+AGG | 0.422845 | 2.3:-64115199 | None:intergenic |
| CCTGCACCACCATTACAAAC+TGG | 0.451169 | 2.3:-64115136 | None:intergenic |
| TTCTTGATTGCATTCAGCTA+TGG | 0.463317 | 2.3:-64115026 | None:intergenic |
| GCATGAAGTAGAAGAAGGTA+AGG | 0.469014 | 2.3:-64114928 | None:intergenic |
| GAAGCTTATAGTAAATCTGC+AGG | 0.482571 | 2.3:+64115163 | MS.gene00716:CDS |
| CCTTCAAATTCTAATAATAG+AGG | 0.488310 | 2.3:+64115199 | MS.gene00716:CDS |
| TTATTAGAATTTGAAGGTGG+AGG | 0.509265 | 2.3:-64115193 | None:intergenic |
| CCAGTTTGTAATGGTGGTGC+AGG | 0.525366 | 2.3:+64115136 | MS.gene00716:CDS |
| GATAAACCAGTTTGTAATGG+TGG | 0.534773 | 2.3:+64115130 | MS.gene00716:CDS |
| AGAGATAAACCAGTTTGTAA+TGG | 0.541924 | 2.3:+64115127 | MS.gene00716:CDS |
| GAAGAGCATGAAGTAGAAGA+AGG | 0.567293 | 2.3:-64114933 | None:intergenic |
| CAAAAGAGATACATTTCTGA+AGG | 0.578266 | 2.3:+64115094 | MS.gene00716:CDS |
| CTATTATTAGAATTTGAAGG+TGG | 0.607589 | 2.3:-64115196 | None:intergenic |
| CATCATGCAAGCATATGCAA+TGG | 0.634173 | 2.3:+64115001 | MS.gene00716:CDS |
| TGGAGTCTGAAATAAGCAGA+AGG | 0.635317 | 2.3:+64115062 | MS.gene00716:CDS |
| GTTTGTAATGGTGGTGCAGG+TGG | 0.645633 | 2.3:+64115139 | MS.gene00716:CDS |
| AAGCTTATAGTAAATCTGCA+GGG | 0.695265 | 2.3:+64115164 | MS.gene00716:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CCTCTATTATTAGAATTTGA+AGG | - | chr2.3:64115202-64115221 | None:intergenic | 25.0% |
| ! | CCTTCAAATTCTAATAATAG+AGG | + | chr2.3:64115199-64115218 | MS.gene00716:CDS | 25.0% |
| ! | CTATTATTAGAATTTGAAGG+TGG | - | chr2.3:64115199-64115218 | None:intergenic | 25.0% |
| AAGCTTATAGTAAATCTGCA+GGG | + | chr2.3:64115164-64115183 | MS.gene00716:CDS | 30.0% | |
| AGAGATAAACCAGTTTGTAA+TGG | + | chr2.3:64115127-64115146 | MS.gene00716:CDS | 30.0% | |
| CAAAAGAGATACATTTCTGA+AGG | + | chr2.3:64115094-64115113 | MS.gene00716:CDS | 30.0% | |
| TCAAGAAGATGAACAATTGA+TGG | + | chr2.3:64115042-64115061 | MS.gene00716:CDS | 30.0% | |
| TTATTAGAATTTGAAGGTGG+AGG | - | chr2.3:64115196-64115215 | None:intergenic | 30.0% | |
| ! | GTTCTAAGTATTATCGTTGT+AGG | + | chr2.3:64115224-64115243 | MS.gene00716:CDS | 30.0% |
| ! | TGAAATAAGCAGAAGGTTTT+TGG | + | chr2.3:64115069-64115088 | MS.gene00716:CDS | 30.0% |
| GAAGCTTATAGTAAATCTGC+AGG | + | chr2.3:64115163-64115182 | MS.gene00716:CDS | 35.0% | |
| GATAAACCAGTTTGTAATGG+TGG | + | chr2.3:64115130-64115149 | MS.gene00716:CDS | 35.0% | |
| TTCTTGATTGCATTCAGCTA+TGG | - | chr2.3:64115029-64115048 | None:intergenic | 35.0% | |
| CATCATGCAAGCATATGCAA+TGG | + | chr2.3:64115001-64115020 | MS.gene00716:CDS | 40.0% | |
| GCATGAAGTAGAAGAAGGTA+AGG | - | chr2.3:64114931-64114950 | None:intergenic | 40.0% | |
| ! | GAAGAGCATGAAGTAGAAGA+AGG | - | chr2.3:64114936-64114955 | None:intergenic | 40.0% |
| ! | TGGAGTCTGAAATAAGCAGA+AGG | + | chr2.3:64115062-64115081 | MS.gene00716:CDS | 40.0% |
| CCTGCACCACCATTACAAAC+TGG | - | chr2.3:64115139-64115158 | None:intergenic | 50.0% | |
| ! | CCAGTTTGTAATGGTGGTGC+AGG | + | chr2.3:64115136-64115155 | MS.gene00716:CDS | 50.0% |
| ! | GTTTGTAATGGTGGTGCAGG+TGG | + | chr2.3:64115139-64115158 | MS.gene00716:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 64114902 | 64115258 | 64114902 | ID=MS.gene00716 |
| chr2.3 | mRNA | 64114902 | 64115258 | 64114902 | ID=MS.gene00716.t1;Parent=MS.gene00716 |
| chr2.3 | exon | 64114902 | 64115258 | 64114902 | ID=MS.gene00716.t1.exon1;Parent=MS.gene00716.t1 |
| chr2.3 | CDS | 64114902 | 64115258 | 64114902 | ID=cds.MS.gene00716.t1;Parent=MS.gene00716.t1 |
| Gene Sequence |
| Protein sequence |
