Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007753.t1 | XP_003606312.1 | 100 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 2.50E-44 | 187.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007753.t1 | O23249 | 94.3 | 88 | 4 | 1 | 1 | 88 | 1 | 87 | 2.4e-43 | 175.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007753.t1 | G7JM76 | 100.0 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 1.8e-44 | 187.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene053749 | MS.gene007753 | PPI |
| MS.gene007753 | MS.gene86296 | PPI |
| MS.gene007753 | MS.gene015893 | PPI |
| MS.gene007753 | MS.gene57583 | PPI |
| MS.gene021573 | MS.gene007753 | PPI |
| MS.gene94095 | MS.gene007753 | PPI |
| MS.gene73923 | MS.gene007753 | PPI |
| MS.gene007753 | MS.gene036003 | PPI |
| MS.gene007753 | MS.gene54070 | PPI |
| MS.gene031852 | MS.gene007753 | PPI |
| MS.gene007753 | MS.gene30776 | PPI |
| MS.gene007753 | MS.gene29062 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007753.t1 | MTR_4g057040 | 100.000 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 1.71e-62 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007753.t1 | AT2G27970 | 90.000 | 80 | 8 | 0 | 1 | 80 | 1 | 80 | 1.45e-51 | 156 |
| MS.gene007753.t1 | AT2G27960 | 97.183 | 71 | 2 | 0 | 1 | 71 | 1 | 71 | 5.79e-49 | 150 |
Find 26 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGGACAACCAGGCCCAGC+AGG | 0.260365 | 4.4:-46242310 | MS.gene007753:CDS |
| CCAGCATGCCCTGCTGGGCC+TGG | 0.333404 | 4.4:+46242301 | None:intergenic |
| AGCCACACATTATGCTCTTC+AGG | 0.335634 | 4.4:-46242356 | MS.gene007753:CDS |
| TTCAGAGAGAAGTCGATTCT+TGG | 0.337969 | 4.4:+46244459 | None:intergenic |
| ATTTGACCAGCATGCCCTGC+TGG | 0.343644 | 4.4:+46242295 | None:intergenic |
| GAGTGCAGCAGAGCCGTGGC+TGG | 0.380036 | 4.4:-46242404 | MS.gene007753:CDS |
| CAGAATGAATGGCGCGCAAT+TGG | 0.392088 | 4.4:-46242426 | MS.gene007753:CDS |
| GGAAGCAACTTCGCAACCTC+TGG | 0.415067 | 4.4:+46244481 | None:intergenic |
| GTTTAATCTTACAGAATGAA+TGG | 0.420697 | 4.4:-46242437 | MS.gene007753:intron |
| TTTGACCAGCATGCCCTGCT+GGG | 0.452510 | 4.4:+46242296 | None:intergenic |
| TCAGAGAGAAGTCGATTCTT+GGG | 0.456322 | 4.4:+46244460 | None:intergenic |
| CGAAATACTTCTCAGAGTAC+TGG | 0.464971 | 4.4:+46244653 | None:intergenic |
| CCAGGCCCAGCAGGGCATGC+TGG | 0.487057 | 4.4:-46242301 | MS.gene007753:CDS |
| TGCTGCTGCTGATAATTCAA+TGG | 0.507626 | 4.4:+46242330 | None:intergenic |
| CAGGACAACCAGGCCCAGCA+GGG | 0.518346 | 4.4:-46242309 | MS.gene007753:CDS |
| AGTGCAGCAGAGCCGTGGCT+GGG | 0.520271 | 4.4:-46242403 | MS.gene007753:CDS |
| AAGAGCATAATGTGTGGCTC+AGG | 0.531062 | 4.4:+46242360 | None:intergenic |
| TCGATGACACTTATGAGTAC+AGG | 0.536322 | 4.4:-46244633 | MS.gene007753:intron |
| GCATGTTGTTCTTCCTCCAG+AGG | 0.545874 | 4.4:-46244497 | MS.gene007753:CDS |
| AGCAACTTCGCAACCTCTGG+AGG | 0.549766 | 4.4:+46244484 | None:intergenic |
| ATTGAATTATCAGCAGCAGC+AGG | 0.557262 | 4.4:-46242328 | MS.gene007753:CDS |
| ATTGGAGTGCAGCAGAGCCG+TGG | 0.573620 | 4.4:-46242408 | MS.gene007753:CDS |
| TCAGCAGCAGCAGGACAACC+AGG | 0.580092 | 4.4:-46242319 | MS.gene007753:CDS |
| TTGCATAATGAACCCAGCCA+CGG | 0.629727 | 4.4:+46242391 | None:intergenic |
| CACACATTATGCTCTTCAGG+AGG | 0.695794 | 4.4:-46242353 | MS.gene007753:CDS |
| CTCCTGAAGAGCATAATGTG+TGG | 0.729925 | 4.4:+46242354 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTTTTTTTTTTTTTT+TGG | - | chr4.4:46242531-46242550 | MS.gene007753:intron | 0.0% |
| !!! | ATTATTTTTGAAAATAAGTA+TGG | - | chr4.4:46244369-46244388 | MS.gene007753:intron | 10.0% |
| !! | AAGTTATCAATTAAAAGTTT+AGG | + | chr4.4:46243424-46243443 | None:intergenic | 15.0% |
| !! | AATTCAAAAAAGAAAAAACT+AGG | + | chr4.4:46242400-46242419 | None:intergenic | 15.0% |
| !! | AGTTATCAATTAAAAGTTAA+AGG | + | chr4.4:46243395-46243414 | None:intergenic | 15.0% |
| !! | AGTTATCAATTAAAAGTTTA+AGG | + | chr4.4:46243423-46243442 | None:intergenic | 15.0% |
| !! | AGTTATCAATTAAAAGTTTA+GGG | + | chr4.4:46243423-46243442 | None:intergenic | 15.0% |
| !! | ATTAAATACACAATGAATTT+AGG | + | chr4.4:46244157-46244176 | None:intergenic | 15.0% |
| !!! | ATTTGTAAAATTTAAGTTCA+AGG | + | chr4.4:46244215-46244234 | None:intergenic | 15.0% |
| !! | AAAACCAAATTAAATCACTT+TGG | + | chr4.4:46243873-46243892 | None:intergenic | 20.0% |
| !! | AGTTATCAGTTAAAAGTTTA+AGG | + | chr4.4:46243365-46243384 | None:intergenic | 20.0% |
| !! | AGTTGTCAATTAAAAGTTTA+AGG | + | chr4.4:46243750-46243769 | None:intergenic | 20.0% |
| !! | ATACAAATTAGGAATTACTT+TGG | + | chr4.4:46244029-46244048 | None:intergenic | 20.0% |
| !!! | AAAGTGATTTAATTTGGTTT+TGG | - | chr4.4:46243872-46243891 | MS.gene007753:intron | 20.0% |
| !!! | AATACTGTATGATTTAAGTT+AGG | + | chr4.4:46243487-46243506 | None:intergenic | 20.0% |
| !!! | GTAATTCCTAATTTGTATTT+TGG | - | chr4.4:46244031-46244050 | MS.gene007753:intron | 20.0% |
| !!! | TAATTTTCTTGTTATTGTTG+TGG | - | chr4.4:46243091-46243110 | MS.gene007753:intron | 20.0% |
| !!! | TATACTGTATGATTTAAGTT+TGG | + | chr4.4:46243338-46243357 | None:intergenic | 20.0% |
| !!! | TTTTTTTGTTGCTGTTTTAT+GGG | - | chr4.4:46242937-46242956 | MS.gene007753:intron | 20.0% |
| !!! | TTTTTTTTGTTGCTGTTTTA+TGG | - | chr4.4:46242936-46242955 | MS.gene007753:intron | 20.0% |
| ! | AAACTTCAAATCACTGTATT+TGG | + | chr4.4:46243947-46243966 | None:intergenic | 25.0% |
| ! | AAAGTGAATTCAGTGAATTA+GGG | + | chr4.4:46243301-46243320 | None:intergenic | 25.0% |
| ! | AACATGCCAAAATACAAATT+AGG | + | chr4.4:46244040-46244059 | None:intergenic | 25.0% |
| ! | AATCACTGTATTTGGATATT+AGG | + | chr4.4:46243939-46243958 | None:intergenic | 25.0% |
| ! | AGTTCATTACACAGTAAATT+TGG | + | chr4.4:46242885-46242904 | None:intergenic | 25.0% |
| ! | GTATTCAGTTATCAGTTAAA+AGG | + | chr4.4:46243511-46243530 | None:intergenic | 25.0% |
| ! | GTTCATTACACAGTAAATTT+GGG | + | chr4.4:46242884-46242903 | None:intergenic | 25.0% |
| ! | GTTTAATCTTACAGAATGAA+TGG | - | chr4.4:46244520-46244539 | MS.gene007753:intron | 25.0% |
| ! | TCAAAATAGATAATACCCTA+AGG | + | chr4.4:46243071-46243090 | None:intergenic | 25.0% |
| ! | TGAAGACTTATATTTGAATC+TGG | - | chr4.4:46242801-46242820 | MS.gene007753:intron | 25.0% |
| ! | TTGAGACGTATATTTGAATT+TGG | - | chr4.4:46243191-46243210 | MS.gene007753:intron | 25.0% |
| !! | TGGGTTTAAAAATCATTTTG+AGG | + | chr4.4:46242865-46242884 | None:intergenic | 25.0% |
| !!! | AATGTTAATTTGTGCATGTT+AGG | - | chr4.4:46242751-46242770 | MS.gene007753:intron | 25.0% |
| !!! | ATGATTTAGGGTTTTGTTAT+GGG | - | chr4.4:46244437-46244456 | MS.gene007753:intron | 25.0% |
| !!! | ATGTTAATTTGTGCATGTTA+GGG | - | chr4.4:46242752-46242771 | MS.gene007753:intron | 25.0% |
| !!! | TATGATTTAGGGTTTTGTTA+TGG | - | chr4.4:46244436-46244455 | MS.gene007753:intron | 25.0% |
| !!! | TATGTTTTAGCGTTTAGATT+AGG | - | chr4.4:46243895-46243914 | MS.gene007753:intron | 25.0% |
| !!! | TGTTTCGTTTTTGTTTTTTC+AGG | - | chr4.4:46242438-46242457 | MS.gene007753:intron | 25.0% |
| AAGCTGAAGCTATATGATTT+AGG | - | chr4.4:46244424-46244443 | MS.gene007753:intron | 30.0% | |
| AATATTACCTTGTGCATGAT+TGG | - | chr4.4:46243829-46243848 | MS.gene007753:intron | 30.0% | |
| AGCTGAAGCTATATGATTTA+GGG | - | chr4.4:46244425-46244444 | MS.gene007753:intron | 30.0% | |
| GAAAGTGAATTCAGTGAATT+AGG | + | chr4.4:46243302-46243321 | None:intergenic | 30.0% | |
| GAGACCAAAGTGATTTAATT+TGG | - | chr4.4:46243866-46243885 | MS.gene007753:intron | 30.0% | |
| GGCATAAAAATCATGTTTAG+AGG | + | chr4.4:46244136-46244155 | None:intergenic | 30.0% | |
| GTATTATCACTGTTGAAAGA+GGG | - | chr4.4:46243157-46243176 | MS.gene007753:intron | 30.0% | |
| TCGGTTTAATATGGAGATAA+TGG | + | chr4.4:46244252-46244271 | None:intergenic | 30.0% | |
| ! | AAACTACAAATCCAAGCTTT+TGG | + | chr4.4:46243269-46243288 | None:intergenic | 30.0% |
| ! | GTTACTCTGATGATGAAATT+GGG | - | chr4.4:46242652-46242671 | MS.gene007753:intron | 30.0% |
| ! | TTACTCTGATGATGAAATTG+GGG | - | chr4.4:46242653-46242672 | MS.gene007753:intron | 30.0% |
| !! | TAGCATTCTCACTTTTGAAA+GGG | - | chr4.4:46244002-46244021 | MS.gene007753:intron | 30.0% |
| !! | TTAGCATTCTCACTTTTGAA+AGG | - | chr4.4:46244001-46244020 | MS.gene007753:intron | 30.0% |
| AACACACTCAATCAAGGAAA+TGG | - | chr4.4:46244271-46244290 | MS.gene007753:intron | 35.0% | |
| AATATTACCCTGTGCATGAT+TGG | - | chr4.4:46243444-46243463 | MS.gene007753:intron | 35.0% | |
| GGTATTATCACTGTTGAAAG+AGG | - | chr4.4:46243156-46243175 | MS.gene007753:intron | 35.0% | |
| GTTGAGTCTTCTGATGAAAA+TGG | - | chr4.4:46242992-46243011 | MS.gene007753:intron | 35.0% | |
| TAATCCTGGTTAAGTCCTTA+GGG | - | chr4.4:46243053-46243072 | MS.gene007753:intron | 35.0% | |
| TGAGTGTGTTCGGTTTAATA+TGG | + | chr4.4:46244261-46244280 | None:intergenic | 35.0% | |
| TTAATCCTGGTTAAGTCCTT+AGG | - | chr4.4:46243052-46243071 | MS.gene007753:intron | 35.0% | |
| TTGGCATGTTTGAGAATTCT+AGG | - | chr4.4:46244050-46244069 | MS.gene007753:intron | 35.0% | |
| TTTCCTTGATTGAGTGTGTT+CGG | + | chr4.4:46244271-46244290 | None:intergenic | 35.0% | |
| ! | AAAAGTTTAAGGCATGCACA+GGG | + | chr4.4:46243789-46243808 | None:intergenic | 35.0% |
| ! | AAACAACAACTCTTGGCTTT+AGG | + | chr4.4:46244110-46244129 | None:intergenic | 35.0% |
| ! | ATCGATTCTAACCAAAAGCT+TGG | - | chr4.4:46243255-46243274 | MS.gene007753:intron | 35.0% |
| ! | ATGTTAGGGTGTACTTTTGA+AGG | - | chr4.4:46242766-46242785 | MS.gene007753:intron | 35.0% |
| ! | CGTTACTCTGATGATGAAAT+TGG | - | chr4.4:46242651-46242670 | MS.gene007753:intron | 35.0% |
| ! | TAAAAGTTTAAGGCATGCAC+AGG | + | chr4.4:46243790-46243809 | None:intergenic | 35.0% |
| ! | TCATGATGATGACTTAATCC+TGG | - | chr4.4:46243039-46243058 | MS.gene007753:intron | 35.0% |
| ! | TGTTTTATGGGTATGTGTGA+TGG | - | chr4.4:46242949-46242968 | MS.gene007753:intron | 35.0% |
| ! | TTGTTGTGGTATTTGATAGC+TGG | - | chr4.4:46243105-46243124 | MS.gene007753:intron | 35.0% |
| !! | AGCATTCTCACTTTTGAAAG+GGG | - | chr4.4:46244003-46244022 | MS.gene007753:intron | 35.0% |
| AAACCGAACACACTCAATCA+AGG | - | chr4.4:46244265-46244284 | MS.gene007753:intron | 40.0% | |
| AAGTGAATCCAATCATGCAC+AGG | + | chr4.4:46243455-46243474 | None:intergenic | 40.0% | |
| AATACCCTAAGGACTTAACC+AGG | + | chr4.4:46243060-46243079 | None:intergenic | 40.0% | |
| AGCTTCAGCTTTGCATTGAA+TGG | + | chr4.4:46244415-46244434 | None:intergenic | 40.0% | |
| AGTGAATCCAATCATGCACA+AGG | + | chr4.4:46243454-46243473 | None:intergenic | 40.0% | |
| AGTGAATCCAATCATGCACA+GGG | + | chr4.4:46243454-46243473 | None:intergenic | 40.0% | |
| ATTGAATTATCAGCAGCAGC+AGG | - | chr4.4:46244629-46244648 | MS.gene007753:intron | 40.0% | |
| CGAAATACTTCTCAGAGTAC+TGG | + | chr4.4:46242307-46242326 | None:intergenic | 40.0% | |
| GAGGCAAAAACAACAACTCT+TGG | + | chr4.4:46244117-46244136 | None:intergenic | 40.0% | |
| GATAGCTGGATATTACTGTG+TGG | - | chr4.4:46243119-46243138 | MS.gene007753:intron | 40.0% | |
| TCGATGACACTTATGAGTAC+AGG | - | chr4.4:46242324-46242343 | MS.gene007753:CDS | 40.0% | |
| TGCTGCTGCTGATAATTCAA+TGG | + | chr4.4:46244630-46244649 | None:intergenic | 40.0% | |
| TGTGTGGCATGTTATTGTTG+TGG | - | chr4.4:46243135-46243154 | MS.gene007753:intron | 40.0% | |
| ! | TCAGAGAGAAGTCGATTCTT+GGG | + | chr4.4:46242500-46242519 | None:intergenic | 40.0% |
| ! | TTCAGAGAGAAGTCGATTCT+TGG | + | chr4.4:46242501-46242520 | None:intergenic | 40.0% |
| AGCCACACATTATGCTCTTC+AGG | - | chr4.4:46244601-46244620 | MS.gene007753:intron | 45.0% | |
| CACACATTATGCTCTTCAGG+AGG | - | chr4.4:46244604-46244623 | MS.gene007753:intron | 45.0% | |
| TTGCATAATGAACCCAGCCA+CGG | + | chr4.4:46244569-46244588 | None:intergenic | 45.0% | |
| ! | AAGAGCATAATGTGTGGCTC+AGG | + | chr4.4:46244600-46244619 | None:intergenic | 45.0% |
| ! | CTCCTGAAGAGCATAATGTG+TGG | + | chr4.4:46244606-46244625 | None:intergenic | 45.0% |
| !!! | TTTTTATTATTATTTTTTTG+TGG | - | chr4.4:46242715-46242734 | MS.gene007753:intron | 5.0% |
| CAGAATGAATGGCGCGCAAT+TGG | - | chr4.4:46244531-46244550 | MS.gene007753:intron | 50.0% | |
| GCATGTTGTTCTTCCTCCAG+AGG | - | chr4.4:46242460-46242479 | MS.gene007753:intron | 50.0% | |
| AGCAACTTCGCAACCTCTGG+AGG | + | chr4.4:46242476-46242495 | None:intergenic | 55.0% | |
| ATTTGACCAGCATGCCCTGC+TGG | + | chr4.4:46244665-46244684 | None:intergenic | 55.0% | |
| GGAAGCAACTTCGCAACCTC+TGG | + | chr4.4:46242479-46242498 | None:intergenic | 55.0% | |
| TTTGACCAGCATGCCCTGCT+GGG | + | chr4.4:46244664-46244683 | None:intergenic | 55.0% | |
| ATTGGAGTGCAGCAGAGCCG+TGG | - | chr4.4:46244549-46244568 | MS.gene007753:intron | 60.0% | |
| TCAGCAGCAGCAGGACAACC+AGG | - | chr4.4:46244638-46244657 | MS.gene007753:CDS | 60.0% | |
| AGTGCAGCAGAGCCGTGGCT+GGG | - | chr4.4:46244554-46244573 | MS.gene007753:intron | 65.0% | |
| CAGGACAACCAGGCCCAGCA+GGG | - | chr4.4:46244648-46244667 | MS.gene007753:CDS | 65.0% | |
| GAGTGCAGCAGAGCCGTGGC+TGG | - | chr4.4:46244553-46244572 | MS.gene007753:intron | 70.0% | |
| GCAGGACAACCAGGCCCAGC+AGG | - | chr4.4:46244647-46244666 | MS.gene007753:CDS | 70.0% | |
| CCAGCATGCCCTGCTGGGCC+TGG | + | chr4.4:46244659-46244678 | None:intergenic | 75.0% | |
| CCAGGCCCAGCAGGGCATGC+TGG | - | chr4.4:46244656-46244675 | MS.gene007753:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 46242293 | 46244686 | 46242293 | ID=MS.gene007753 |
| chr4.4 | mRNA | 46242293 | 46244686 | 46242293 | ID=MS.gene007753.t1;Parent=MS.gene007753 |
| chr4.4 | exon | 46244634 | 46244686 | 46244634 | ID=MS.gene007753.t1.exon1;Parent=MS.gene007753.t1 |
| chr4.4 | CDS | 46244634 | 46244686 | 46244634 | ID=cds.MS.gene007753.t1;Parent=MS.gene007753.t1 |
| chr4.4 | exon | 46244459 | 46244519 | 46244459 | ID=MS.gene007753.t1.exon2;Parent=MS.gene007753.t1 |
| chr4.4 | CDS | 46244459 | 46244519 | 46244459 | ID=cds.MS.gene007753.t1;Parent=MS.gene007753.t1 |
| chr4.4 | exon | 46242293 | 46242445 | 46242293 | ID=MS.gene007753.t1.exon3;Parent=MS.gene007753.t1 |
| chr4.4 | CDS | 46242293 | 46242445 | 46242293 | ID=cds.MS.gene007753.t1;Parent=MS.gene007753.t1 |
| Gene Sequence |
| Protein sequence |
