AlfalfaGEDB | MS.gene00829

M. sativa cultivar XinJiangDaYe / MS.gene00829

KEGG: K02115
GO: GO:0003674 GO:0003824 GO:0005215 GO:0005216 GO:0005261 GO:0005575 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009165 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0015075 GO:0015077 GO:0015078 GO:0015252 GO:0015267 GO:0015318 GO:0015672 GO:0015985 GO:0015986 GO:0016740 GO:0016772 GO:0016776 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0022803 GO:0022857 GO:0022890 GO:0032991 GO:0033178 GO:0034220 GO:0034641 GO:0034654 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0045261 GO:0046034 GO:0046390 GO:0046483 GO:0046933 GO:0051179 GO:0051234 GO:0055085 GO:0055086 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
PFAM: PF00231.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00829.t1	ACJ84643.1	93	374	26	0	1	374	1	374	1.60E-185	658.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00829.t1	P28552	91.9	369	30	0	1	369	1	369	4.2e-174	612.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00829.t1	B7FIR4	93.0	374	26	0	1	374	1	374	1.2e-185	658.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene035640	MS.gene00829	PPI
MS.gene57960	MS.gene00829	PPI
MS.gene012472	MS.gene00829	PPI
MS.gene055477	MS.gene00829	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00829.t1	MTR_2g023290	97.952	293	6	0	82	374	729	1021	0.0	587
MS.gene00829.t1	MTR_3g052270	68.155	336	101	2	44	373	12	347	4.26e-168	473
MS.gene00829.t1	MTR_1588s0010	99.010	101	1	0	1	101	1	101	2.09e-65	203
MS.gene00829.t1	MTR_4g129790	29.301	372	193	13	9	373	17	325	1.86e-26	108

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00829.t1	AT4G04640	81.117	376	66	3	1	374	1	373	0.0	599
MS.gene00829.t1	AT1G15700	70.725	345	100	1	30	373	39	383	2.38e-177	498
MS.gene00829.t1	AT2G33040	31.790	324	164	12	57	373	48	321	7.70e-27	109

Find 61 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTTAGACCACACTGAATT+TGG	0.110416	2.3:+64900830	None:intergenic
TATTATCAGTGTTGGTAAAA+AGG	0.215367	2.3:-64900476	MS.gene00829:CDS
GGTGAAATCTGTGATATTAA+TGG	0.242769	2.3:-64900235	MS.gene00829:CDS
GCTCAAGAAGCTGTTGTTAA+TGG	0.249925	2.3:-64900721	MS.gene00829:CDS
ATTGCTGAGTTGAAAGTTCT+TGG	0.268699	2.3:-64900511	MS.gene00829:CDS
TCAAATCTTGAGGGCTTTGC+AGG	0.276662	2.3:-64900038	MS.gene00829:CDS
GTGACAGTGGAAGCAAAGTA+TGG	0.339355	2.3:+64900263	None:intergenic
GGAAGCAAAGTATGGATTAT+AGG	0.339933	2.3:+64900271	None:intergenic
ATATCACAGATTTCACCTTT+TGG	0.359402	2.3:+64900241	None:intergenic
AACCTATCAACAGGTATATA+AGG	0.362640	2.3:+64900430	None:intergenic
TCCTGCTCAAACTGCAAAAT+CGG	0.366440	2.3:+64900106	None:intergenic
TCTTAGACCACACTGAATTT+GGG	0.374759	2.3:+64900831	None:intergenic
ACTGGTCTAAGCTTAGTTAG+AGG	0.375333	2.3:+64900622	None:intergenic
CAGCACCAGCAACAATTTCC+AGG	0.387882	2.3:+64899885	None:intergenic
TGAATTTGGGTTGGTCTTGA+AGG	0.393465	2.3:+64900844	None:intergenic
GACCTTATATACCTGTTGAT+AGG	0.399954	2.3:-64900432	MS.gene00829:CDS
CTGTTGAAAGAGATGCTGTT+AGG	0.407775	2.3:-64900150	MS.gene00829:CDS
TGCATCAGGTAATGAAGGTT+TGG	0.412967	2.3:+64900939	None:intergenic
CCTGTTGATAGGTTTCTTGA+AGG	0.420186	2.3:-64900421	MS.gene00829:CDS
TTCAGGTTAACAACGAAAGA+AGG	0.449455	2.3:-64900181	MS.gene00829:CDS
TTTGAACAGTCAAATCTTGA+GGG	0.462626	2.3:-64900047	MS.gene00829:CDS
GTTGCTCTTGTTGTTTGCAC+TGG	0.463249	2.3:-64900592	MS.gene00829:CDS
TGTTGCTGGTGCTGATGCCT+TGG	0.464852	2.3:-64899876	MS.gene00829:CDS
CCTTCAAGAAACCTATCAAC+AGG	0.466272	2.3:+64900421	None:intergenic
TCAACTAGTGTCTGTGAGAA+AGG	0.467803	2.3:+64900694	None:intergenic
ATTTGACTGTTCAAATAAAG+TGG	0.475054	2.3:+64900055	None:intergenic
TAAGATCACTGGTGAAATCC+TGG	0.483893	2.3:-64899903	MS.gene00829:CDS
AGTGCATCAAGAATCTGAAC+AGG	0.485687	2.3:+64900082	None:intergenic
GAAATCCTGGAAATTGTTGC+TGG	0.495138	2.3:-64899890	MS.gene00829:CDS
AAGGAGAGGTTAGATGCATC+AGG	0.495737	2.3:+64900925	None:intergenic
GTTGTTTGCACTGGTGATAG+AGG	0.498449	2.3:-64900583	MS.gene00829:CDS
GTGCTTCTTTAGCAGTAGGA+AGG	0.507770	2.3:+64900392	None:intergenic
TTGCTTCCACTGTCACCAAA+AGG	0.509640	2.3:-64900256	MS.gene00829:CDS
TTAGATGCATCAGGTAATGA+AGG	0.514624	2.3:+64900934	None:intergenic
GTTGATAGGTTTCTTGAAGG+TGG	0.518256	2.3:-64900418	MS.gene00829:CDS
TTTCACCAGTGATCTTAGCT+TGG	0.530031	2.3:+64899909	None:intergenic
GTTTGTGCTTCTTTAGCAGT+AGG	0.537662	2.3:+64900388	None:intergenic
ATTTGAACAGTCAAATCTTG+AGG	0.540431	2.3:-64900048	MS.gene00829:CDS
CAACTTCTTCACTAACAAAG+AGG	0.544704	2.3:+64900344	None:intergenic
GATTACACTATTATCAGTGT+TGG	0.547808	2.3:-64900484	MS.gene00829:CDS
ATCAGGTAATGAAGGTTTGG+AGG	0.549600	2.3:+64900942	None:intergenic
GCTGTTCGTTGATGCTGTAA+AGG	0.551485	2.3:+64900668	None:intergenic
TTTGTGAAGGAAGCTGAAAA+GGG	0.553199	2.3:+64900887	None:intergenic
AGACCACACTGAATTTGGGT+TGG	0.556222	2.3:+64900835	None:intergenic
GTTGAAGAAGAATTTGTCAA+GGG	0.556839	2.3:-64899948	MS.gene00829:CDS
AGGGCTGAGAGCTGAACGGA+AGG	0.577278	2.3:+64900906	None:intergenic
AGTTGAAGAAGAATTTGTCA+AGG	0.578085	2.3:-64899949	MS.gene00829:CDS
CAACGCCAAGCTAAGATCAC+TGG	0.583862	2.3:-64899914	MS.gene00829:CDS
TAGAGGAGCTTCGATATCGT+CGG	0.592502	2.3:+64900639	None:intergenic
AACTTCTTCACTAACAAAGA+GGG	0.594771	2.3:+64900345	None:intergenic
GGTGATAGAGGTCTCTGTGG+TGG	0.597310	2.3:-64900571	MS.gene00829:CDS
CACTGAAGCAATGAAGCTTG+TGG	0.611051	2.3:-64900764	MS.gene00829:CDS
TTGCTCAAATATCACCATGT+TGG	0.614531	2.3:-64900968	MS.gene00829:CDS
TGAGAGCTGAACGGAAGGAG+AGG	0.630596	2.3:+64900911	None:intergenic
AAACTGTGTTGATGCTGCTG+AGG	0.639433	2.3:-64900212	MS.gene00829:CDS
GAAAAGGGCTGAGAGCTGAA+CGG	0.641643	2.3:+64900902	None:intergenic
TATAAATACACTCTATACCA+AGG	0.641733	2.3:+64899859	None:intergenic
CAGGTATATAAGGTCTACGA+AGG	0.649710	2.3:+64900440	None:intergenic
AGGTTTGGAGGAGACCAACA+TGG	0.658294	2.3:+64900954	None:intergenic
ACTGGTGATAGAGGTCTCTG+TGG	0.722605	2.3:-64900574	MS.gene00829:CDS
AGACCAACCCAAATTCAGTG+TGG	0.755194	2.3:-64900838	MS.gene00829:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTGAAAAAAGCTGAAAAT+AGG	-	chr2.3:64900310-64900329	MS.gene00829:CDS	20.0%
!	ATTTGACTGTTCAAATAAAG+TGG	+	chr2.3:64900792-64900811	None:intergenic	25.0%
!!!	ATTATCAGTGTTGGTAAAAA+GGG	-	chr2.3:64900369-64900388	MS.gene00829:CDS	25.0%
!!!	TATTATCAGTGTTGGTAAAA+AGG	-	chr2.3:64900368-64900387	MS.gene00829:CDS	25.0%
	AACCTATCAACAGGTATATA+AGG	+	chr2.3:64900417-64900436	None:intergenic	30.0%
	AACTTCTTCACTAACAAAGA+GGG	+	chr2.3:64900502-64900521	None:intergenic	30.0%
	AGTTGAAGAAGAATTTGTCA+AGG	-	chr2.3:64900895-64900914	MS.gene00829:CDS	30.0%
	ATTTGAACAGTCAAATCTTG+AGG	-	chr2.3:64900796-64900815	MS.gene00829:CDS	30.0%
	GATTACACTATTATCAGTGT+TGG	-	chr2.3:64900360-64900379	MS.gene00829:CDS	30.0%
	GGTGAAATCTGTGATATTAA+TGG	-	chr2.3:64900609-64900628	MS.gene00829:CDS	30.0%
	GTTGAAGAAGAATTTGTCAA+GGG	-	chr2.3:64900896-64900915	MS.gene00829:CDS	30.0%
	TTTGAACAGTCAAATCTTGA+GGG	-	chr2.3:64900797-64900816	MS.gene00829:CDS	30.0%
!	ATATCACAGATTTCACCTTT+TGG	+	chr2.3:64900606-64900625	None:intergenic	30.0%
!!	TGTTTTTGACAGAGTCAATA+CGG	+	chr2.3:64900050-64900069	None:intergenic	30.0%
	CAACTTCTTCACTAACAAAG+AGG	+	chr2.3:64900503-64900522	None:intergenic	35.0%
	GACCTTATATACCTGTTGAT+AGG	-	chr2.3:64900412-64900431	MS.gene00829:CDS	35.0%
	TCTTAGACCACACTGAATTT+GGG	+	chr2.3:64900016-64900035	None:intergenic	35.0%
	TTAGATGCATCAGGTAATGA+AGG	+	chr2.3:64899913-64899932	None:intergenic	35.0%
	TTGCTCAAATATCACCATGT+TGG	-	chr2.3:64899876-64899895	MS.gene00829:CDS	35.0%
	TTTGTGAAGGAAGCTGAAAA+GGG	+	chr2.3:64899960-64899979	None:intergenic	35.0%
!	ATTGCTGAGTTGAAAGTTCT+TGG	-	chr2.3:64900333-64900352	MS.gene00829:CDS	35.0%
!	GGAAGCAAAGTATGGATTAT+AGG	+	chr2.3:64900576-64900595	None:intergenic	35.0%
!	TTCAGGTTAACAACGAAAGA+AGG	-	chr2.3:64900663-64900682	MS.gene00829:CDS	35.0%
!	TTTTGTGAAGGAAGCTGAAA+AGG	+	chr2.3:64899961-64899980	None:intergenic	35.0%
!!	GAAGATGATGAGTTTTGTGA+AGG	+	chr2.3:64899973-64899992	None:intergenic	35.0%
!!!	ACAAGAGCAACTTTTTTCAC+TGG	+	chr2.3:64900243-64900262	None:intergenic	35.0%
	AGTGCATCAAGAATCTGAAC+AGG	+	chr2.3:64900765-64900784	None:intergenic	40.0%
	ATCAGGTAATGAAGGTTTGG+AGG	+	chr2.3:64899905-64899924	None:intergenic	40.0%
	CAGGTATATAAGGTCTACGA+AGG	+	chr2.3:64900407-64900426	None:intergenic	40.0%
	CCTTCAAGAAACCTATCAAC+AGG	+	chr2.3:64900426-64900445	None:intergenic	40.0%
	CTCTTAGACCACACTGAATT+TGG	+	chr2.3:64900017-64900036	None:intergenic	40.0%
	CTGTTGAAAGAGATGCTGTT+AGG	-	chr2.3:64900694-64900713	MS.gene00829:CDS	40.0%
	GAAATCCTGGAAATTGTTGC+TGG	-	chr2.3:64900954-64900973	MS.gene00829:CDS	40.0%
	GCTCAAGAAGCTGTTGTTAA+TGG	-	chr2.3:64900123-64900142	MS.gene00829:CDS	40.0%
	GTTTGTGCTTCTTTAGCAGT+AGG	+	chr2.3:64900459-64900478	None:intergenic	40.0%
	TAAGATCACTGGTGAAATCC+TGG	-	chr2.3:64900941-64900960	MS.gene00829:CDS	40.0%
	TCAACTAGTGTCTGTGAGAA+AGG	+	chr2.3:64900153-64900172	None:intergenic	40.0%
	TCCTGCTCAAACTGCAAAAT+CGG	+	chr2.3:64900741-64900760	None:intergenic	40.0%
	TGCATCAGGTAATGAAGGTT+TGG	+	chr2.3:64899908-64899927	None:intergenic	40.0%
	TTTCACCAGTGATCTTAGCT+TGG	+	chr2.3:64900938-64900957	None:intergenic	40.0%
!	ACTGGTCTAAGCTTAGTTAG+AGG	+	chr2.3:64900225-64900244	None:intergenic	40.0%
!	CCTGTTGATAGGTTTCTTGA+AGG	-	chr2.3:64900423-64900442	MS.gene00829:CDS	40.0%
!	GTTGATAGGTTTCTTGAAGG+TGG	-	chr2.3:64900426-64900445	MS.gene00829:CDS	40.0%
!!	ATTTCACCTTTTGGTGACAG+TGG	+	chr2.3:64900597-64900616	None:intergenic	40.0%
!!	TGAATTTGGGTTGGTCTTGA+AGG	+	chr2.3:64900003-64900022	None:intergenic	40.0%
	AAGGAGAGGTTAGATGCATC+AGG	+	chr2.3:64899922-64899941	None:intergenic	45.0%
	AGACCAACCCAAATTCAGTG+TGG	-	chr2.3:64900006-64900025	MS.gene00829:CDS	45.0%
	AGACCACACTGAATTTGGGT+TGG	+	chr2.3:64900012-64900031	None:intergenic	45.0%
	CACTGAAGCAATGAAGCTTG+TGG	-	chr2.3:64900080-64900099	MS.gene00829:CDS	45.0%
	GCTGTTCGTTGATGCTGTAA+AGG	+	chr2.3:64900179-64900198	None:intergenic	45.0%
	GTGACAGTGGAAGCAAAGTA+TGG	+	chr2.3:64900584-64900603	None:intergenic	45.0%
	GTGCTTCTTTAGCAGTAGGA+AGG	+	chr2.3:64900455-64900474	None:intergenic	45.0%
	TAGAGGAGCTTCGATATCGT+CGG	+	chr2.3:64900208-64900227	None:intergenic	45.0%
	TCAAATCTTGAGGGCTTTGC+AGG	-	chr2.3:64900806-64900825	MS.gene00829:CDS	45.0%
	TTGCTTCCACTGTCACCAAA+AGG	-	chr2.3:64900588-64900607	MS.gene00829:CDS	45.0%
!	AAACTGTGTTGATGCTGCTG+AGG	-	chr2.3:64900632-64900651	MS.gene00829:CDS	45.0%
!	GTTGTTTGCACTGGTGATAG+AGG	-	chr2.3:64900261-64900280	MS.gene00829:CDS	45.0%
!!	GTTGCTCTTGTTGTTTGCAC+TGG	-	chr2.3:64900252-64900271	MS.gene00829:CDS	45.0%
	ACTGGTGATAGAGGTCTCTG+TGG	-	chr2.3:64900270-64900289	MS.gene00829:CDS	50.0%
	AGGTTTGGAGGAGACCAACA+TGG	+	chr2.3:64899893-64899912	None:intergenic	50.0%
	CAACGCCAAGCTAAGATCAC+TGG	-	chr2.3:64900930-64900949	MS.gene00829:CDS	50.0%
	CAGCACCAGCAACAATTTCC+AGG	+	chr2.3:64900962-64900981	None:intergenic	50.0%
	GAAAAGGGCTGAGAGCTGAA+CGG	+	chr2.3:64899945-64899964	None:intergenic	50.0%
!	CTGCTGAGGATGAGCTTTTC+AGG	-	chr2.3:64900646-64900665	MS.gene00829:CDS	50.0%
!!	GCCGATTTTGCAGTTTGAGC+AGG	-	chr2.3:64900737-64900756	MS.gene00829:CDS	50.0%
	GGTGATAGAGGTCTCTGTGG+TGG	-	chr2.3:64900273-64900292	MS.gene00829:CDS	55.0%
	TGAGAGCTGAACGGAAGGAG+AGG	+	chr2.3:64899936-64899955	None:intergenic	55.0%
!!	TGTTGCTGGTGCTGATGCCT+TGG	-	chr2.3:64900968-64900987	MS.gene00829:CDS	55.0%
	AGGGCTGAGAGCTGAACGGA+AGG	+	chr2.3:64899941-64899960	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	64899871	64900995	64899871	ID=MS.gene00829
chr2.3	mRNA	64899871	64900995	64899871	ID=MS.gene00829.t1;Parent=MS.gene00829
chr2.3	exon	64899871	64900995	64899871	ID=MS.gene00829.t1.exon1;Parent=MS.gene00829.t1
chr2.3	CDS	64899871	64900995	64899871	ID=cds.MS.gene00829.t1;Parent=MS.gene00829.t1

Gene Sequence

>MS.gene00829

ATGTCTTGCTCAAATATCACCATGTTGGTCTCCTCCAAACCTTCATTACCTGATGCATCTAACCTCTCCTTCCGTTCAGCTCTCAGCCCTTTTCAGCTTCCTTCACAAAACTCATCATCTTCTTGCACTCCTTCAAGACCAACCCAAATTCAGTGTGGTCTAAGAGAACTTAGAAACCGTATTGACTCTGTCAAAAACACTCAGAAAATCACTGAAGCAATGAAGCTTGTGGCTGCTGCAAAAGTCAGAAGAGCTCAAGAAGCTGTTGTTAATGGCAGACCTTTCTCACAGACACTAGTTGAAGTCCTTTACAGCATCAACGAACAGCTTCAAACCGACGATATCGAAGCTCCTCTAACTAAGCTTAGACCAGTGAAAAAAGTTGCTCTTGTTGTTTGCACTGGTGATAGAGGTCTCTGTGGTGGATTCAACAATGCAATATTGAAAAAAGCTGAAAATAGGATTGCTGAGTTGAAAGTTCTTGGACTTGATTACACTATTATCAGTGTTGGTAAAAAGGGTAACTCTTATTTCCTTCGTAGACCTTATATACCTGTTGATAGGTTTCTTGAAGGTGGAAACCTTCCTACTGCTAAAGAAGCACAAACTATTGCTGATGATGTTTTCTCCCTCTTTGTTAGTGAAGAAGTTGATAAAGTTGAACTATTGTACACTAAATTTGTGTCATTAGTGAAATCAGAGCCTATAATCCATACTTTGCTTCCACTGTCACCAAAAGGTGAAATCTGTGATATTAATGGAAACTGTGTTGATGCTGCTGAGGATGAGCTTTTCAGGTTAACAACGAAAGAAGGAAAGTTAACTGTTGAAAGAGATGCTGTTAGGTCTAAGACGATTGATTTTTCGCCGATTTTGCAGTTTGAGCAGGATCCTGTTCAGATTCTTGATGCACTTTTGCCACTTTATTTGAACAGTCAAATCTTGAGGGCTTTGCAGGAATCACTTGCTAGTGAATTAGCTGCTAGAATGAGTGCTATGAGTAGTGCTTGTGATAATGCTATTGAGTTGAAGAAGAATTTGTCAAGGGTTTATAATAGACAACGCCAAGCTAAGATCACTGGTGAAATCCTGGAAATTGTTGCTGGTGCTGATGCCTTGGTATAG

Protein sequence

>MS.gene00829.t1

MSCSNITMLVSSKPSLPDASNLSFRSALSPFQLPSQNSSSSCTPSRPTQIQCGLRELRNRIDSVKNTQKITEAMKLVAAAKVRRAQEAVVNGRPFSQTLVEVLYSINEQLQTDDIEAPLTKLRPVKKVALVVCTGDRGLCGGFNNAILKKAENRIAELKVLGLDYTIISVGKKGNSYFLRRPYIPVDRFLEGGNLPTAKEAQTIADDVFSLFVSEEVDKVELLYTKFVSLVKSEPIIHTLLPLSPKGEICDINGNCVDAAEDELFRLTTKEGKLTVERDAVRSKTIDFSPILQFEQDPVQILDALLPLYLNSQILRALQESLASELAARMSAMSSACDNAIELKKNLSRVYNRQRQAKITGEILEIVAGADALV