Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012352.t1 | RHN64698.1 | 88.4 | 327 | 35 | 2 | 1 | 326 | 14 | 338 | 1.90E-169 | 605.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012352.t1 | Q9SW00 | 53.7 | 324 | 147 | 3 | 1 | 323 | 59 | 380 | 1.4e-96 | 354.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012352.t1 | G7JUB4 | 88.4 | 327 | 35 | 2 | 1 | 326 | 58 | 382 | 1.3e-169 | 605.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene032779 | MS.gene012352 | PPI |
| MS.gene066288 | MS.gene012352 | PPI |
| MS.gene99026 | MS.gene012352 | PPI |
| MS.gene012352 | MS.gene49595 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012352.t1 | MTR_4g127510 | 88.379 | 327 | 35 | 2 | 1 | 326 | 58 | 382 | 0.0 | 596 |
| MS.gene012352.t1 | MTR_2g037770 | 69.632 | 326 | 96 | 2 | 1 | 326 | 60 | 382 | 2.40e-166 | 468 |
| MS.gene012352.t1 | MTR_2g037790 | 64.832 | 327 | 111 | 3 | 1 | 326 | 64 | 387 | 1.95e-152 | 433 |
| MS.gene012352.t1 | MTR_2g037790 | 64.832 | 327 | 111 | 3 | 1 | 326 | 61 | 384 | 2.33e-152 | 432 |
| MS.gene012352.t1 | MTR_4g127490 | 65.031 | 326 | 110 | 3 | 1 | 323 | 69 | 393 | 3.20e-152 | 433 |
| MS.gene012352.t1 | MTR_4g127490 | 65.031 | 326 | 110 | 3 | 1 | 323 | 67 | 391 | 4.78e-152 | 432 |
| MS.gene012352.t1 | MTR_2g037770 | 70.370 | 243 | 69 | 2 | 1 | 243 | 60 | 299 | 5.96e-121 | 351 |
| MS.gene012352.t1 | MTR_2g037770 | 70.370 | 243 | 69 | 2 | 1 | 243 | 61 | 300 | 6.65e-121 | 350 |
| MS.gene012352.t1 | MTR_2g037770 | 71.226 | 212 | 58 | 2 | 1 | 212 | 60 | 268 | 2.69e-106 | 311 |
| MS.gene012352.t1 | MTR_4g064110 | 27.124 | 306 | 187 | 9 | 22 | 303 | 111 | 404 | 1.80e-20 | 92.0 |
| MS.gene012352.t1 | MTR_7g061830 | 26.733 | 303 | 192 | 10 | 22 | 303 | 115 | 408 | 4.81e-16 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012352.t1 | AT4G25850 | 53.538 | 325 | 148 | 3 | 1 | 324 | 59 | 381 | 4.81e-118 | 345 |
| MS.gene012352.t1 | AT5G57240 | 51.543 | 324 | 154 | 3 | 1 | 323 | 54 | 375 | 6.09e-117 | 343 |
| MS.gene012352.t1 | AT5G57240 | 51.543 | 324 | 154 | 3 | 1 | 323 | 54 | 375 | 6.09e-117 | 343 |
| MS.gene012352.t1 | AT5G57240 | 51.543 | 324 | 154 | 3 | 1 | 323 | 54 | 375 | 6.09e-117 | 343 |
| MS.gene012352.t1 | AT4G25850 | 53.538 | 325 | 148 | 3 | 1 | 324 | 161 | 483 | 1.72e-116 | 345 |
| MS.gene012352.t1 | AT4G25860 | 51.692 | 325 | 154 | 3 | 1 | 324 | 60 | 382 | 2.48e-112 | 331 |
| MS.gene012352.t1 | AT4G25860 | 51.692 | 325 | 154 | 3 | 1 | 324 | 54 | 376 | 3.37e-112 | 330 |
| MS.gene012352.t1 | AT5G57240 | 47.578 | 351 | 156 | 4 | 1 | 325 | 54 | 402 | 7.98e-111 | 328 |
| MS.gene012352.t1 | AT5G57240 | 47.578 | 351 | 156 | 4 | 1 | 325 | 54 | 402 | 7.98e-111 | 328 |
| MS.gene012352.t1 | AT4G25850 | 55.238 | 210 | 92 | 2 | 1 | 209 | 59 | 267 | 1.29e-72 | 226 |
| MS.gene012352.t1 | AT5G59420 | 25.902 | 305 | 190 | 9 | 22 | 302 | 110 | 402 | 9.27e-17 | 81.3 |
Find 91 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGTCAAAAGGGGAAATT+TGG | 0.145591 | 8.3:-3659914 | MS.gene012352:CDS |
| ACTACACGACCACAGAAATT+TGG | 0.188123 | 8.3:-3671175 | MS.gene012352:CDS |
| AACAGAATCTGCTCTTGTTT+GGG | 0.210716 | 8.3:-3660033 | MS.gene012352:CDS |
| AGACAATCTTTCTTTGGATT+TGG | 0.216954 | 8.3:-3669151 | MS.gene012352:CDS |
| AGAAATCTGAATAAGAAATT+TGG | 0.242852 | 8.3:+3669256 | None:intergenic |
| CAACAGAATCTGCTCTTGTT+TGG | 0.245208 | 8.3:-3660034 | MS.gene012352:CDS |
| CATATGTCTCTCCATGATTA+TGG | 0.251319 | 8.3:+3669290 | None:intergenic |
| CTGCTACCAGGCCTGTTTCT+AGG | 0.260032 | 8.3:+3669188 | None:intergenic |
| GCTCCCTACAATCCCATTCT+TGG | 0.262230 | 8.3:-3671148 | MS.gene012352:CDS |
| TTCCAATTCCTGGTATTGAT+TGG | 0.273123 | 8.3:-3669234 | MS.gene012352:CDS |
| TACACTAGACAATCTTTCTT+TGG | 0.278088 | 8.3:-3669157 | MS.gene012352:CDS |
| GGAAATCGAAGACGGATAAA+AGG | 0.297030 | 8.3:-3669124 | MS.gene012352:CDS |
| GACAATCTTTCTTTGGATTT+GGG | 0.297690 | 8.3:-3669150 | MS.gene012352:CDS |
| TTCAGATTTCTTCCAATTCC+TGG | 0.299189 | 8.3:-3669244 | MS.gene012352:CDS |
| TTTATAACATTGACGGTCAT+TGG | 0.319680 | 8.3:-3669069 | MS.gene012352:CDS |
| TCCAATTCCTGGTATTGATT+GGG | 0.344320 | 8.3:-3669233 | MS.gene012352:CDS |
| GATCCTTAACAAAAGGAGTA+TGG | 0.346607 | 8.3:+3660257 | None:intergenic |
| TCCCCAATCGTTGCTTATGA+TGG | 0.379990 | 8.3:+3659995 | None:intergenic |
| AGGGTGGCAATAGGAGCATC+TGG | 0.384339 | 8.3:+3659819 | None:intergenic |
| GATCCAGATAAATAATTATA+GGG | 0.385472 | 8.3:+3659800 | None:intergenic |
| ACCCAATCAATACCAGGAAT+TGG | 0.388589 | 8.3:+3669232 | None:intergenic |
| AATAATTATAGGGTGGCAAT+AGG | 0.420289 | 8.3:+3659810 | None:intergenic |
| TGAGTCACTATAAAATGTTT+AGG | 0.424628 | 8.3:+3659888 | None:intergenic |
| TCTCCACTTCCAAAGTTTGT+TGG | 0.426253 | 8.3:-3669503 | MS.gene012352:intron |
| ACTATCCGGTGCCTAGAAAC+AGG | 0.426852 | 8.3:-3669199 | MS.gene012352:CDS |
| AATGTTCTTTATAACATTGA+CGG | 0.438522 | 8.3:-3669076 | MS.gene012352:CDS |
| TTATAACATTGACGGTCATT+GGG | 0.448664 | 8.3:-3669068 | MS.gene012352:CDS |
| ATGGTGGGTTTCACCAAGAA+TGG | 0.451451 | 8.3:+3671135 | None:intergenic |
| TTAGAGAAAGAGAGTCAAAA+GGG | 0.454350 | 8.3:-3659923 | MS.gene012352:CDS |
| AGCCATCATAAGCAACGATT+GGG | 0.455090 | 8.3:-3659997 | MS.gene012352:CDS |
| AGAGTCAAAAGGGGAAATTT+GGG | 0.456782 | 8.3:-3659913 | MS.gene012352:CDS |
| AATCGTTGCTTATGATGGCT+TGG | 0.457816 | 8.3:+3660000 | None:intergenic |
| CTTAGAGAAAGAGAGTCAAA+AGG | 0.461372 | 8.3:-3659924 | MS.gene012352:CDS |
| CCTTAACGTCTTGCTCGAAC+AGG | 0.465737 | 8.3:-3671098 | MS.gene012352:intron |
| TCTTTCTTTGGATTTGGGTC+GGG | 0.467324 | 8.3:-3669145 | MS.gene012352:CDS |
| ATCTTTCTTTGGATTTGGGT+CGG | 0.469873 | 8.3:-3669146 | MS.gene012352:CDS |
| ACCTCTAGATCCTTAACAAA+AGG | 0.474417 | 8.3:+3660250 | None:intergenic |
| CAAGTCTACTCCTGTGGCTG+TGG | 0.475561 | 8.3:+3671267 | None:intergenic |
| TACCATTGAAGCTGTGACTT+AGG | 0.485158 | 8.3:+3671307 | None:intergenic |
| TTCACCAAGAATGGGATTGT+AGG | 0.487044 | 8.3:+3671144 | None:intergenic |
| GAAACCCACCATGTTTCTAA+AGG | 0.495301 | 8.3:-3671124 | MS.gene012352:CDS |
| AAACCCACCATGTTTCTAAA+GGG | 0.496469 | 8.3:-3671123 | MS.gene012352:CDS |
| TTACCAACAAACTTTGGAAG+TGG | 0.498053 | 8.3:+3669500 | None:intergenic |
| TGGTGGGTTTCACCAAGAAT+GGG | 0.499357 | 8.3:+3671136 | None:intergenic |
| CTCATAGCAATGAAGATGGC+TGG | 0.502171 | 8.3:-3659869 | MS.gene012352:CDS |
| GCAGGTTTCACACCATCCTC+AGG | 0.503834 | 8.3:-3669582 | MS.gene012352:intron |
| CCTGTTCGAGCAAGACGTTA+AGG | 0.505938 | 8.3:+3671098 | None:intergenic |
| AAGCCATCATAAGCAACGAT+TGG | 0.513927 | 8.3:-3659998 | MS.gene012352:CDS |
| TCACCAAGAATGGGATTGTA+GGG | 0.514686 | 8.3:+3671145 | None:intergenic |
| CAACTGCACCTCCATAATCA+TGG | 0.525416 | 8.3:-3669301 | MS.gene012352:CDS |
| TGCCTAAGTCACAGCTTCAA+TGG | 0.525691 | 8.3:-3671309 | MS.gene012352:CDS |
| AAGTCACAGCTTCAATGGTA+TGG | 0.534057 | 8.3:-3671304 | MS.gene012352:CDS |
| GGGTTGGCAATGTCACTATC+CGG | 0.534777 | 8.3:-3669213 | MS.gene012352:CDS |
| TGTGTCTATTCCACAGCCAC+AGG | 0.535366 | 8.3:-3671277 | MS.gene012352:CDS |
| GAAATCGAAGACGGATAAAA+GGG | 0.537567 | 8.3:-3669123 | MS.gene012352:CDS |
| ACTGTTCACCAATTCAAAAG+TGG | 0.544922 | 8.3:-3659845 | MS.gene012352:CDS |
| ATTCCTGGTATTGATTGGGT+TGG | 0.551107 | 8.3:-3669229 | MS.gene012352:CDS |
| GACTTATATCAGTAGTAGCA+TGG | 0.557193 | 8.3:-3671207 | MS.gene012352:CDS |
| ACAGAATCTGCTCTTGTTTG+GGG | 0.557754 | 8.3:-3660032 | MS.gene012352:CDS |
| ACATTGACGGTCATTGGGAT+AGG | 0.562966 | 8.3:-3669063 | MS.gene012352:intron |
| TGTAGCTCAATTCTGCTACC+AGG | 0.567744 | 8.3:+3669176 | None:intergenic |
| TTTCCATCTGATCAGTGGCA+TGG | 0.571356 | 8.3:+3669549 | None:intergenic |
| CCAGGCCTGTTTCTAGGCAC+CGG | 0.578301 | 8.3:+3669194 | None:intergenic |
| AAATTATAAATGCAGGTACA+GGG | 0.580423 | 8.3:-3669351 | MS.gene012352:intron |
| GTGACTCATAGCAATGAAGA+TGG | 0.582352 | 8.3:-3659873 | MS.gene012352:CDS |
| TAAGGTTCCCTTTAGAAACA+TGG | 0.582927 | 8.3:+3671116 | None:intergenic |
| ATTCCATGCCACTGATCAGA+TGG | 0.593014 | 8.3:-3669552 | MS.gene012352:CDS |
| GTTTAGCAAGTCTACTCCTG+TGG | 0.609510 | 8.3:+3671261 | None:intergenic |
| CCGGTGCCTAGAAACAGGCC+TGG | 0.615502 | 8.3:-3669194 | MS.gene012352:CDS |
| CTGTTCACCAATTCAAAAGT+GGG | 0.616115 | 8.3:-3659844 | MS.gene012352:CDS |
| GTTCCCTTTAGAAACATGGT+GGG | 0.616437 | 8.3:+3671120 | None:intergenic |
| CAAACAAGAGCAGATTCTGT+TGG | 0.621500 | 8.3:+3660035 | None:intergenic |
| CGATTGGGGAAAAGCAAGAG+AGG | 0.622181 | 8.3:-3659982 | MS.gene012352:CDS |
| TTGCTAAACAACATCAACAA+TGG | 0.626138 | 8.3:-3671247 | MS.gene012352:CDS |
| GGGTGGCAATAGGAGCATCT+GGG | 0.627495 | 8.3:+3659820 | None:intergenic |
| GGTTCCCTTTAGAAACATGG+TGG | 0.629919 | 8.3:+3671119 | None:intergenic |
| GGGGTAGAAGTTGACATGCA+TGG | 0.630449 | 8.3:-3669331 | MS.gene012352:CDS |
| AAGAGAGGCAAAGAACACTC+TGG | 0.634477 | 8.3:-3659967 | MS.gene012352:CDS |
| CAAACTTTGGAAGTGGAGAG+TGG | 0.635431 | 8.3:+3669507 | None:intergenic |
| TTGCCAACCCAATCAATACC+AGG | 0.642684 | 8.3:+3669226 | None:intergenic |
| TTGGGTCGGGAAATCGAAGA+CGG | 0.645859 | 8.3:-3669132 | MS.gene012352:CDS |
| CCAGATAAATAATTATAGGG+TGG | 0.646503 | 8.3:+3659803 | None:intergenic |
| ATGTCTCTCCATGATTATGG+AGG | 0.650308 | 8.3:+3669293 | None:intergenic |
| GCCATCATAAGCAACGATTG+GGG | 0.655456 | 8.3:-3659996 | MS.gene012352:CDS |
| AATTATAAATGCAGGTACAG+GGG | 0.662926 | 8.3:-3669350 | MS.gene012352:intron |
| TAGAGAAAGAGAGTCAAAAG+GGG | 0.666484 | 8.3:-3659922 | MS.gene012352:CDS |
| GGAGCAAATCCAAATTTCTG+TGG | 0.677683 | 8.3:+3671166 | None:intergenic |
| AGAGGCAAAGAACACTCTGG+AGG | 0.685308 | 8.3:-3659964 | MS.gene012352:CDS |
| GGAATGCAGATACCTGAGGA+TGG | 0.705504 | 8.3:+3669570 | None:intergenic |
| ACACTCTGGAGGAAACTCAG+AGG | 0.724153 | 8.3:-3659953 | MS.gene012352:CDS |
| GCATGGAATGCAGATACCTG+AGG | 0.732347 | 8.3:+3669566 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 3659815 | 3671332 | 3659815 | ID=MS.gene012352 |
| chr8.3 | mRNA | 3659815 | 3671332 | 3659815 | ID=MS.gene012352.t1;Parent=MS.gene012352 |
| chr8.3 | exon | 3671099 | 3671332 | 3671099 | ID=MS.gene012352.t1.exon1;Parent=MS.gene012352.t1 |
| chr8.3 | CDS | 3671099 | 3671332 | 3671099 | ID=cds.MS.gene012352.t1;Parent=MS.gene012352.t1 |
| chr8.3 | exon | 3669504 | 3669600 | 3669504 | ID=MS.gene012352.t1.exon2;Parent=MS.gene012352.t1 |
| chr8.3 | CDS | 3669504 | 3669600 | 3669504 | ID=cds.MS.gene012352.t1;Parent=MS.gene012352.t1 |
| chr8.3 | exon | 3669064 | 3669358 | 3669064 | ID=MS.gene012352.t1.exon3;Parent=MS.gene012352.t1 |
| chr8.3 | CDS | 3669064 | 3669358 | 3669064 | ID=cds.MS.gene012352.t1;Parent=MS.gene012352.t1 |
| chr8.3 | exon | 3660252 | 3660354 | 3660252 | ID=MS.gene012352.t1.exon4;Parent=MS.gene012352.t1 |
| chr8.3 | CDS | 3660252 | 3660354 | 3660252 | ID=cds.MS.gene012352.t1;Parent=MS.gene012352.t1 |
| chr8.3 | exon | 3659815 | 3660066 | 3659815 | ID=MS.gene012352.t1.exon5;Parent=MS.gene012352.t1 |
| chr8.3 | CDS | 3659815 | 3660066 | 3659815 | ID=cds.MS.gene012352.t1;Parent=MS.gene012352.t1 |
| Gene Sequence |
| Protein sequence |
