AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012767

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012767.t1 MTR_4g134280 96.533 375 11 1 122 496 2 374 0.0 745
MS.gene012767.t1 MTR_2g027560 73.626 455 114 3 26 477 21 472 0.0 707
MS.gene012767.t1 MTR_1g010120 53.066 473 212 5 13 483 8 472 2.10e-172 494
MS.gene012767.t1 MTR_4g124440 49.080 489 229 10 19 496 11 490 2.74e-158 459
MS.gene012767.t1 MTR_7g085390 47.925 482 235 6 13 488 6 477 3.65e-157 455
MS.gene012767.t1 MTR_3g108600 51.835 436 188 5 31 464 29 444 1.61e-153 446
MS.gene012767.t1 MTR_6g032820 51.716 437 193 7 13 440 10 437 2.67e-153 444
MS.gene012767.t1 MTR_1g053320 50.580 431 202 5 33 456 30 456 1.84e-148 433
MS.gene012767.t1 MTR_4g112025 46.970 462 235 4 33 488 31 488 3.70e-148 434
MS.gene012767.t1 MTR_3g031770 48.724 431 212 4 32 460 26 449 2.39e-141 416
MS.gene012767.t1 MTR_8g092070 46.861 446 229 5 29 470 30 471 4.26e-141 418
MS.gene012767.t1 MTR_3g095050 45.244 431 226 7 32 458 33 457 2.21e-128 382
MS.gene012767.t1 MTR_5g085580 33.624 458 260 15 33 459 25 469 1.71e-66 222
MS.gene012767.t1 MTR_6g088885 31.466 464 293 13 5 457 6 455 1.04e-65 221
MS.gene012767.t1 MTR_1g056640 32.762 467 265 16 32 459 37 493 3.27e-64 216
MS.gene012767.t1 MTR_4g088155 34.667 450 257 20 31 456 21 457 6.97e-64 214
MS.gene012767.t1 MTR_8g085720 32.166 457 281 16 14 456 7 448 1.04e-61 210
MS.gene012767.t1 MTR_4g069940 32.385 457 268 17 29 461 25 464 1.82e-61 208
MS.gene012767.t1 MTR_8g012400 32.574 439 270 15 34 456 30 458 8.47e-61 206
MS.gene012767.t1 MTR_3g116510 33.111 450 270 16 29 461 24 459 1.66e-60 206
MS.gene012767.t1 MTR_1g031900 32.574 439 270 14 30 456 21 445 2.90e-57 198
MS.gene012767.t1 MTR_1g031900 32.574 439 270 14 30 456 21 445 2.93e-57 198
MS.gene012767.t1 MTR_3g083580 30.000 470 296 16 14 470 7 456 8.51e-56 194
MS.gene012767.t1 MTR_3g080410 37.048 332 194 10 25 348 17 341 1.89e-55 189
MS.gene012767.t1 MTR_4g076570 34.259 324 202 7 29 350 18 332 3.45e-55 189
MS.gene012767.t1 MTR_7g053500 31.448 442 274 16 34 456 27 458 2.54e-54 189
MS.gene012767.t1 MTR_4g132280 30.064 469 287 15 33 479 45 494 9.34e-54 189
MS.gene012767.t1 MTR_1g052430 34.483 319 196 9 33 348 32 340 9.05e-52 181
MS.gene012767.t1 MTR_3g065460 29.978 457 286 15 16 456 7 445 1.94e-51 182
MS.gene012767.t1 MTR_2g034470 33.231 325 206 6 28 350 25 340 2.49e-51 179
MS.gene012767.t1 MTR_8g102340 31.981 419 252 16 91 486 50 458 3.62e-51 181
MS.gene012767.t1 MTR_2g034440 33.436 326 205 7 28 350 17 333 3.83e-51 177
MS.gene012767.t1 MTR_2g034480 32.092 349 225 7 7 353 2 340 4.75e-51 178
MS.gene012767.t1 MTR_7g081350 34.412 340 205 10 14 350 11 335 1.35e-50 176
MS.gene012767.t1 MTR_4g076255 35.276 326 187 11 32 352 24 330 3.43e-50 174
MS.gene012767.t1 MTR_4g083500 30.261 499 290 19 33 488 44 527 9.88e-50 178
MS.gene012767.t1 MTR_7g026340 29.778 450 280 19 25 455 15 447 1.44e-49 176
MS.gene012767.t1 MTR_2g034470 33.775 302 189 6 51 350 13 305 3.74e-49 172
MS.gene012767.t1 MTR_5g078200 29.515 454 292 14 16 456 6 444 4.02e-49 179
MS.gene012767.t1 MTR_8g012400 31.783 387 238 15 86 456 2 378 4.47e-48 171
MS.gene012767.t1 MTR_4g010200 34.877 324 193 10 34 348 34 348 1.11e-47 169
MS.gene012767.t1 MTR_7g053500 32.564 390 234 16 86 456 2 381 2.08e-47 169
MS.gene012767.t1 MTR_4g084480 32.931 331 207 11 31 355 23 344 1.57e-46 166
MS.gene012767.t1 MTR_4g084480 32.931 331 207 11 31 355 30 351 1.67e-46 166
MS.gene012767.t1 MTR_4g084480 32.931 331 207 11 31 355 30 351 2.03e-46 166
MS.gene012767.t1 MTR_5g078200 29.717 424 270 14 46 456 3 411 4.17e-46 170
MS.gene012767.t1 MTR_1g022160 28.326 466 283 16 33 459 24 477 8.99e-46 166
MS.gene012767.t1 MTR_1g022160 28.510 463 280 16 33 456 24 474 1.75e-45 166
MS.gene012767.t1 MTR_7g081370 33.333 348 200 11 10 350 1 323 2.52e-45 161
MS.gene012767.t1 MTR_4g052970 33.425 365 212 15 32 388 28 369 2.97e-45 163
MS.gene012767.t1 MTR_4g076470 31.765 340 207 12 13 348 6 324 2.17e-44 159
MS.gene012767.t1 MTR_4g114850 32.716 324 200 10 34 348 27 341 4.20e-44 159
MS.gene012767.t1 MTR_4g076430 31.105 344 212 12 13 352 6 328 4.90e-44 158
MS.gene012767.t1 MTR_1g077990 32.727 330 206 11 32 354 30 350 5.05e-44 160
MS.gene012767.t1 MTR_4g114850 32.716 324 200 10 34 348 27 341 5.49e-44 159
MS.gene012767.t1 MTR_4g114850 32.716 324 200 10 34 348 27 341 5.62e-44 159
MS.gene012767.t1 MTR_1g022160 28.289 456 276 16 33 449 24 467 8.41e-44 161
MS.gene012767.t1 MTR_4g076500 32.166 314 194 9 32 340 77 376 5.54e-43 157
MS.gene012767.t1 MTR_3g116510 33.929 336 194 14 143 461 1 325 7.42e-43 155
MS.gene012767.t1 MTR_4g076440 30.725 345 216 12 10 350 1 326 1.06e-42 154
MS.gene012767.t1 MTR_4g076490 30.994 342 213 12 28 365 92 414 3.39e-42 155
MS.gene012767.t1 MTR_1g007810 33.233 331 201 13 19 340 1 320 7.90e-42 153
MS.gene012767.t1 MTR_2g086530 29.156 391 245 16 9 387 2 372 5.56e-41 151
MS.gene012767.t1 MTR_2g086530 30.028 363 225 15 34 387 9 351 2.10e-40 149
MS.gene012767.t1 MTR_2g019345 31.707 328 206 12 34 354 25 341 9.60e-40 148
MS.gene012767.t1 MTR_1g022160 31.481 324 207 9 33 348 24 340 5.11e-39 147
MS.gene012767.t1 MTR_5g081720 30.408 319 191 14 58 362 62 363 2.59e-30 122
MS.gene012767.t1 MTR_8g085440 44.792 96 47 4 364 456 23 115 2.29e-15 72.8
MS.gene012767.t1 MTR_4g036680 36.364 99 58 3 252 350 82 175 1.15e-14 73.6
MS.gene012767.t1 MTR_4g036775 36.000 100 59 3 252 351 82 176 3.60e-14 72.0
MS.gene012767.t1 MTR_8g085420 42.105 95 49 4 364 455 23 114 1.98e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012767.t1 AT4G17180 70.575 452 125 2 17 468 9 452 0.0 673
MS.gene012767.t1 AT4G31140 49.269 479 235 6 13 487 6 480 1.58e-165 477
MS.gene012767.t1 AT5G58090 51.522 460 213 5 31 487 20 472 2.26e-157 456
MS.gene012767.t1 AT2G19440 48.233 481 234 8 16 487 4 478 3.06e-152 443
MS.gene012767.t1 AT2G19440 48.233 481 234 8 16 487 4 478 3.06e-152 443
MS.gene012767.t1 AT3G24330 46.996 466 236 7 13 471 19 480 5.40e-152 443
MS.gene012767.t1 AT1G64760 49.234 457 217 8 10 460 5 452 9.51e-152 442
MS.gene012767.t1 AT1G64760 49.234 457 217 8 10 460 5 452 9.51e-152 442
MS.gene012767.t1 AT5G20870 49.460 463 207 10 33 475 30 485 1.03e-145 427
MS.gene012767.t1 AT5G18220 45.940 468 231 9 31 490 28 481 6.45e-144 422
MS.gene012767.t1 AT3G04010 45.000 460 241 8 12 465 13 466 9.22e-143 419
MS.gene012767.t1 AT5G64790 44.444 459 243 6 10 460 1 455 1.05e-134 398
MS.gene012767.t1 AT5G58480 45.796 452 233 7 29 477 22 464 6.31e-132 391
MS.gene012767.t1 AT5G55180 35.135 444 260 15 33 459 27 459 1.46e-71 235
MS.gene012767.t1 AT2G05790 34.565 460 257 15 32 459 24 471 5.18e-71 234
MS.gene012767.t1 AT5G55180 35.172 435 254 15 33 450 27 450 1.50e-68 227
MS.gene012767.t1 AT4G26830 32.589 448 263 15 33 459 25 454 3.27e-65 218
MS.gene012767.t1 AT4G26830 32.589 448 263 15 33 459 29 458 3.73e-65 218
MS.gene012767.t1 AT3G07320 34.921 441 258 18 34 456 26 455 1.13e-61 209
MS.gene012767.t1 AT2G01630 32.273 440 270 14 30 456 20 444 4.86e-61 208
MS.gene012767.t1 AT2G01630 31.954 435 268 14 30 451 20 439 1.77e-59 202
MS.gene012767.t1 AT3G13560 32.063 446 274 15 33 465 26 455 3.30e-58 201
MS.gene012767.t1 AT3G13560 32.063 446 274 15 33 465 26 455 3.30e-58 201
MS.gene012767.t1 AT3G13560 32.063 446 274 15 33 465 26 455 3.30e-58 201
MS.gene012767.t1 AT3G13560 32.063 446 274 15 33 465 26 455 3.30e-58 201
MS.gene012767.t1 AT3G13560 32.063 446 274 15 33 465 26 455 3.30e-58 201
MS.gene012767.t1 AT5G24318 31.250 448 270 19 29 456 26 455 1.07e-57 198
MS.gene012767.t1 AT5G56590 30.044 456 287 14 33 469 26 468 1.63e-57 199
MS.gene012767.t1 AT1G11820 29.668 482 299 15 33 488 44 511 1.92e-56 196
MS.gene012767.t1 AT3G23770 33.185 449 265 18 31 456 35 471 2.85e-56 195
MS.gene012767.t1 AT4G29360 29.730 481 290 14 31 474 35 504 1.57e-55 194
MS.gene012767.t1 AT4G29360 29.730 481 290 14 31 474 25 494 1.68e-55 194
MS.gene012767.t1 AT5G24318 31.151 443 267 19 29 451 26 450 2.75e-55 191
MS.gene012767.t1 AT3G55430 32.653 441 264 21 31 456 27 449 6.30e-55 191
MS.gene012767.t1 AT4G34480 33.257 439 267 15 30 456 23 447 6.75e-55 192
MS.gene012767.t1 AT2G16230 30.612 490 303 17 26 492 18 493 2.00e-54 191
MS.gene012767.t1 AT1G11820 30.233 430 274 13 33 450 44 459 3.38e-53 187
MS.gene012767.t1 AT3G57270 34.356 326 203 7 29 352 23 339 3.71e-53 183
MS.gene012767.t1 AT3G57260 33.237 346 214 9 10 352 7 338 3.89e-53 182
MS.gene012767.t1 AT1G66250 29.769 477 297 14 26 479 25 486 4.88e-53 187
MS.gene012767.t1 AT3G57260 33.237 346 214 9 10 352 23 354 6.01e-53 182
MS.gene012767.t1 AT4G14080 31.839 446 272 17 31 456 40 473 7.18e-53 186
MS.gene012767.t1 AT4G16260 32.362 343 216 9 13 350 1 332 4.80e-49 172
MS.gene012767.t1 AT4G18340 31.250 368 232 12 11 367 10 367 8.57e-49 172
MS.gene012767.t1 AT4G18340 31.250 368 232 12 11 367 10 367 1.65e-48 172
MS.gene012767.t1 AT2G39640 31.648 455 268 21 16 454 13 440 1.29e-47 173
MS.gene012767.t1 AT4G16260 34.098 305 188 8 48 350 10 303 2.26e-47 167
MS.gene012767.t1 AT2G01630 31.476 359 224 11 30 378 20 366 2.82e-47 169
MS.gene012767.t1 AT3G15800 34.146 328 199 10 34 352 44 363 5.00e-46 165
MS.gene012767.t1 AT5G42720 33.428 353 215 13 11 352 3 346 9.00e-46 166
MS.gene012767.t1 AT4G34480 36.036 333 197 10 29 353 22 346 1.24e-45 163
MS.gene012767.t1 AT1G30080 33.030 330 196 12 31 348 34 350 2.65e-45 163
MS.gene012767.t1 AT1G30080 33.030 330 196 12 31 348 34 350 3.93e-45 163
MS.gene012767.t1 AT3G57240 32.609 322 199 9 33 352 35 340 2.34e-44 159
MS.gene012767.t1 AT2G26600 31.928 332 206 11 34 356 35 355 3.72e-44 160
MS.gene012767.t1 AT2G27500 29.781 366 229 12 33 391 29 373 5.20e-44 159
MS.gene012767.t1 AT2G27500 30.387 362 224 12 33 387 29 369 1.19e-43 159
MS.gene012767.t1 AT5G24318 32.081 346 217 13 29 367 26 360 4.27e-43 157
MS.gene012767.t1 AT1G32860 32.143 336 207 12 32 356 28 353 7.04e-42 155
MS.gene012767.t1 AT5G24318 32.622 328 188 15 148 456 3 316 1.63e-40 148
MS.gene012767.t1 AT1G33220 33.115 305 189 8 27 326 28 322 1.19e-39 146
MS.gene012767.t1 AT1G11820 30.000 330 215 9 33 353 44 366 4.62e-39 146
MS.gene012767.t1 AT3G61810 28.783 337 213 9 29 353 51 372 9.02e-39 145
MS.gene012767.t1 AT5G20390 31.949 313 195 9 33 340 33 332 1.13e-38 144
MS.gene012767.t1 AT1G77790 31.715 309 195 9 47 350 44 341 1.29e-38 144
MS.gene012767.t1 AT5G42100 32.070 343 214 13 20 352 15 348 1.74e-38 145
MS.gene012767.t1 AT5G42100 32.070 343 214 13 20 352 15 348 2.04e-38 145
MS.gene012767.t1 AT5G20340 33.016 315 193 9 31 340 41 342 2.16e-38 144
MS.gene012767.t1 AT1G77780 29.730 296 195 6 47 340 38 322 6.04e-38 142
MS.gene012767.t1 AT5G20560 30.794 315 205 8 29 340 30 334 2.27e-37 140
MS.gene012767.t1 AT4G34480 32.659 346 209 14 123 456 3 336 3.77e-37 141
MS.gene012767.t1 AT5G20330 31.348 319 201 9 27 340 28 333 5.05e-37 139
MS.gene012767.t1 AT2G27500 30.128 312 190 12 83 387 1 291 3.17e-34 131
MS.gene012767.t1 AT3G46570 32.493 357 208 15 13 357 14 349 4.46e-34 132
MS.gene012767.t1 AT2G26600 31.250 272 167 11 94 356 1 261 6.46e-31 121
MS.gene012767.t1 AT3G55780 24.620 329 226 9 38 357 43 358 1.41e-28 117

Find 125 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTATAGTTATTGGAGAAAT+TGG 0.161486 8.1:+541865 MS.gene012767:CDS
GGGTAGATATTAACAATAAA+TGG 0.180808 8.1:-541679 None:intergenic
CCCCTTCTGCTTCTGATTTG+TGG 0.212209 8.1:+540513 MS.gene012767:CDS
GGTCAAATTCCTATAGTTAT+TGG 0.217381 8.1:+541856 MS.gene012767:CDS
TTTCCAAGGCCAAGGTTTAA+TGG 0.241450 8.1:-542114 None:intergenic
GTCGCAGCACTCAGTAAAAT+TGG 0.243518 8.1:+541829 MS.gene012767:CDS
ATTTATAGCAATGCATTTGA+TGG 0.264940 8.1:+541790 MS.gene012767:CDS
GAACTAGCTGAGTCATGATT+TGG 0.293607 8.1:-541633 None:intergenic
TTCAGTATCTTCCGTCTAAA+TGG 0.295863 8.1:+542163 MS.gene012767:CDS
GATTGTACAACTCTTGATTA+TGG 0.306971 8.1:+542264 MS.gene012767:CDS
GGAGGTTCATGCAATGGAAT+TGG 0.323892 8.1:+542285 MS.gene012767:CDS
CCTTCTCTAAGGATTGTTGT+AGG 0.325174 8.1:-541513 None:intergenic
GGTCTTATCAACCATGTTCT+TGG 0.327129 8.1:+541946 MS.gene012767:CDS
AGCCAGCTTTGCCTTCTCTA+AGG 0.335120 8.1:-541524 None:intergenic
TCAACTGGTGGATCTCAAAT+AGG 0.335178 8.1:+542468 MS.gene012767:CDS
CGGTGTAAGCAGAGACGTTC+TGG 0.340269 8.1:-540541 None:intergenic
GGTAGATATTAACAATAAAT+GGG 0.351539 8.1:-541678 None:intergenic
CCAATTTCTCCAATAACTAT+AGG 0.363185 8.1:-541865 None:intergenic
GAGGTTCATGCAATGGAATT+GGG 0.364355 8.1:+542286 MS.gene012767:CDS
AATCAGCCATAGGAGTAAAC+TGG 0.364459 8.1:+540312 MS.gene012767:CDS
GATATCAGCACCACCTTTCC+CGG 0.368497 8.1:-540561 None:intergenic
GGAAAGGTGGTGCTGATATC+AGG 0.370412 8.1:+540564 MS.gene012767:CDS
GCACTCAGTAAAATTGGATA+TGG 0.381123 8.1:+541835 MS.gene012767:CDS
CAAGACTATGCGTTCTTTGA+GGG 0.405455 8.1:+541736 MS.gene012767:CDS
CAAAAGAAACAACATCTCAT+TGG 0.414587 8.1:-540483 None:intergenic
TTTGACATTAATAATGATGT+TGG 0.417994 8.1:+540262 MS.gene012767:CDS
GTCGCCGACTGAAGGGTCTC+TGG 0.419124 8.1:-542406 None:intergenic
AGCAGAGGCTGATGTTCTTA+AGG 0.419618 8.1:+540421 MS.gene012767:CDS
TATCCATTAAACCTTGGCCT+TGG 0.421251 8.1:+542111 MS.gene012767:CDS
ACAGAGCCTTGCAACAGGTT+TGG 0.424953 8.1:+540227 MS.gene012767:CDS
GTGGAATTCAGGTGATGGTT+GGG 0.429711 8.1:+540456 MS.gene012767:CDS
CCATTTCTTAAAAGTTATAA+CGG 0.439246 8.1:+541454 MS.gene012767:CDS
CAGTCGAAGGCTTCAATTTG+CGG 0.446370 8.1:-540349 None:intergenic
AACTAGCTGAGTCATGATTT+GGG 0.447469 8.1:-541632 None:intergenic
ATCCTTAGAGAAGGCAAAGC+TGG 0.449396 8.1:+541522 MS.gene012767:CDS
ACAAGACTATGCGTTCTTTG+AGG 0.449531 8.1:+541735 MS.gene012767:CDS
ATATCCATAGGAGGAGCATT+AGG 0.450841 8.1:-541991 None:intergenic
TGGCCTTCAGATGGAGCAAT+TGG 0.450952 8.1:+541889 MS.gene012767:CDS
TTGACATTAATAATGATGTT+GGG 0.450983 8.1:+540263 MS.gene012767:CDS
AATTACAGGTATGTTGCTGT+TGG 0.453794 8.1:+541424 MS.gene012767:intron
TAATGATGTTGGGTTTATGC+TGG 0.454194 8.1:+540273 MS.gene012767:CDS
AAAAGAAACAACATCTCATT+GGG 0.456722 8.1:-540482 None:intergenic
CGTGCCCCAGTTTACTCCTA+TGG 0.462495 8.1:-540318 None:intergenic
GGAGCAAAGAGTATTCTTCC+AGG 0.464462 8.1:+542039 MS.gene012767:CDS
CAAAGAACGCATAGTCTTGT+GGG 0.465598 8.1:-541732 None:intergenic
ACTCATGGGAAGTGGAATTC+AGG 0.466332 8.1:+540445 MS.gene012767:CDS
CTGTTCAGCCTTCTTGATGA+AGG 0.466581 8.1:+542018 MS.gene012767:CDS
GTAACAAGGGGACACCTTTG+AGG 0.473169 8.1:+541968 MS.gene012767:CDS
GAGGCCTAATGCTCCTCCTA+TGG 0.474425 8.1:+541987 MS.gene012767:CDS
ATGGCTGATTCTGTCAATAC+AGG 0.476202 8.1:-540299 None:intergenic
CAAATTGAAGCCTTCGACTG+TGG 0.484496 8.1:+540352 MS.gene012767:CDS
GATAAAGCTACACGTTCAAC+TGG 0.485931 8.1:+542453 MS.gene012767:CDS
AGTGGAATTCAGGTGATGGT+TGG 0.486153 8.1:+540455 MS.gene012767:CDS
GTCATGATTTGGGTTAGATC+AGG 0.487046 8.1:-541622 None:intergenic
CAACCATGTTCTTGGTAACA+AGG 0.487104 8.1:+541954 MS.gene012767:CDS
TAATGACAGAGCCTTGCAAC+AGG 0.487466 8.1:+540222 None:intergenic
TGATGTTCTTAAGGCACTCA+TGG 0.496691 8.1:+540430 MS.gene012767:CDS
TCTTCAATTCCTTGTTTCCA+AGG 0.501671 8.1:-542128 None:intergenic
TGTTGTAGGTTGAGTACTGC+AGG 0.501811 8.1:-541499 None:intergenic
TATCCCTTGTGAAGGAATTG+AGG 0.502246 8.1:-541593 None:intergenic
CAATGTCTTTCAAAACTACC+TGG 0.504549 8.1:-542057 None:intergenic
ATTCCTTGTTTCCAAGGCCA+AGG 0.514113 8.1:-542122 None:intergenic
AAACCTTGGCCTTGGAAACA+AGG 0.515265 8.1:+542119 MS.gene012767:CDS
AAACGACAGTCGCCGACTGA+AGG 0.516511 8.1:-542414 None:intergenic
CTCAAATAGGATATAGTTGG+TGG 0.517574 8.1:+542481 MS.gene012767:CDS
GCCTTGCAACAGGTTTGGTA+TGG 0.517906 8.1:+540232 MS.gene012767:CDS
GCACCAATTGCTCCATCTGA+AGG 0.518393 8.1:-541892 None:intergenic
GAAATTGGTTGGCCTTCAGA+TGG 0.520140 8.1:+541880 MS.gene012767:CDS
TAGTTATTGGAGAAATTGGT+TGG 0.521897 8.1:+541869 MS.gene012767:CDS
CGTCGTAGAGGCTGAGGAAA+GGG 0.522100 8.1:-541699 None:intergenic
GTTCAGTTCCTCAACTCAAA+CGG 0.528913 8.1:+541652 MS.gene012767:CDS
GATCTCAAATAGGATATAGT+TGG 0.530437 8.1:+542478 MS.gene012767:CDS
ATGGGGAACCGTTTGAGTTG+AGG 0.536855 8.1:-541660 None:intergenic
TGAGTACTGCAGGCATGACC+AGG 0.540544 8.1:-541489 None:intergenic
AACGACAGTCGCCGACTGAA+GGG 0.543117 8.1:-542413 None:intergenic
ATCAGCCATAGGAGTAAACT+GGG 0.543941 8.1:+540313 MS.gene012767:CDS
CTTAAGGCACTCATGGGAAG+TGG 0.544463 8.1:+540437 MS.gene012767:CDS
GTCTCTGCTTACACCGGGAA+AGG 0.548221 8.1:+540548 MS.gene012767:CDS
TAACGGTCAATTTCAGAACC+TGG 0.548760 8.1:+541471 MS.gene012767:CDS
ATATTTGAACCATCTGTGAC+AGG 0.551387 8.1:-541769 None:intergenic
GCTAAGTATCCATTAAACCT+TGG 0.553117 8.1:+542105 MS.gene012767:CDS
TCAAAGAACGCATAGTCTTG+TGG 0.554316 8.1:-541733 None:intergenic
TCTTTGCTCCTTCATCAAGA+AGG 0.555297 8.1:-542026 None:intergenic
AGTGAAACTGTTTGAAGCAG+AGG 0.555786 8.1:+540406 MS.gene012767:CDS
CAGTATCGTCGTAGAGGCTG+AGG 0.558216 8.1:-541705 None:intergenic
AACCATGTTCTTGGTAACAA+GGG 0.564205 8.1:+541955 MS.gene012767:CDS
CTGAACAGATAGATATCCAT+AGG 0.564300 8.1:-542003 None:intergenic
GATTATGGAGGTTCATGCAA+TGG 0.567791 8.1:+542279 MS.gene012767:CDS
TCATAGACATCTGCATTGCA+TGG 0.573085 8.1:-541568 None:intergenic
TCCACAAATCAGAAGCAGAA+GGG 0.574897 8.1:-540514 None:intergenic
CCACAAATCAGAAGCAGAAG+GGG 0.577752 8.1:-540513 None:intergenic
TGCAATGGAATTGGGGACAA+AGG 0.581526 8.1:+542294 MS.gene012767:CDS
GGAGGAGCATTAGGCCTCAA+AGG 0.591534 8.1:-541982 None:intergenic
ATCCACAAATCAGAAGCAGA+AGG 0.594435 8.1:-540515 None:intergenic
TCCCCTTGTTACCAAGAACA+TGG 0.599301 8.1:-541957 None:intergenic
CTTACCACACACCATTTAGA+CGG 0.600170 8.1:-542174 None:intergenic
AAAGCTACACGTTCAACTGG+TGG 0.601725 8.1:+542456 MS.gene012767:CDS
TCGTCGTAGAGGCTGAGGAA+AGG 0.602157 8.1:-541700 None:intergenic
TGTACAACTCTTGATTATGG+AGG 0.604592 8.1:+542267 MS.gene012767:CDS
AGGTTCATGCAATGGAATTG+GGG 0.605748 8.1:+542287 MS.gene012767:CDS
TGGTTTGAGTATTAATAATG+TGG 0.606241 8.1:+542209 MS.gene012767:CDS
GATGTTCTTAAGGCACTCAT+GGG 0.608418 8.1:+540431 MS.gene012767:CDS
TCTGCTTACACCGGGAAAGG+TGG 0.610018 8.1:+540551 MS.gene012767:CDS
AAGAGATCAACCACAGTCGA+AGG 0.614973 8.1:-540362 None:intergenic
ACTACTCATCCTGTCACAGA+TGG 0.616505 8.1:+541760 MS.gene012767:CDS
GCCATACCAAACCTGTTGCA+AGG 0.618952 8.1:-540233 None:intergenic
CAAATCAGAAGCAGAAGGGG+AGG 0.619027 8.1:-540510 None:intergenic
GTGGTAAGTTCTAAGTCAAG+TGG 0.623574 8.1:+542189 MS.gene012767:CDS
CCTACAACAATCCTTAGAGA+AGG 0.625958 8.1:+541513 MS.gene012767:CDS
GGACGGAATATCCCTTGTGA+AGG 0.626622 8.1:-541601 None:intergenic
ACCATGTTCTTGGTAACAAG+GGG 0.635252 8.1:+541956 MS.gene012767:CDS
AACAGATAGATATCCATAGG+AGG 0.636789 8.1:-542000 None:intergenic
CAGAACGTCTCTGCTTACAC+CGG 0.637608 8.1:+540542 MS.gene012767:CDS
ACTTCCAGAGACCCTTCAGT+CGG 0.638338 8.1:+542402 MS.gene012767:CDS
GAATCCTCAATTCCTTCACA+AGG 0.640274 8.1:+541589 MS.gene012767:CDS
AGAACGTCTCTGCTTACACC+GGG 0.644702 8.1:+540543 MS.gene012767:CDS
CATAGGAGTAAACTGGGGCA+CGG 0.648386 8.1:+540319 MS.gene012767:CDS
GGAAATCAGTATCGTCGTAG+AGG 0.649323 8.1:-541711 None:intergenic
GTAGATATTAACAATAAATG+GGG 0.652349 8.1:-541677 None:intergenic
TGATTTGGGTTAGATCAGGA+CGG 0.652522 8.1:-541618 None:intergenic
GTATTGACAGAATCAGCCAT+AGG 0.655460 8.1:+540302 MS.gene012767:CDS
AATCCTCAATTCCTTCACAA+GGG 0.658418 8.1:+541590 MS.gene012767:CDS
GGGAAGTGGAATTCAGGTGA+TGG 0.670388 8.1:+540451 MS.gene012767:CDS
TCTTCCGTCTAAATGGTGTG+TGG 0.709729 8.1:+542170 MS.gene012767:CDS
TCAGCCATAGGAGTAAACTG+GGG 0.815969 8.1:+540314 MS.gene012767:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTTTTACAACAAATAATA+AGG + chr8.1:540739-540758 MS.gene012767:intron 15.0%
!! CATAACAAAAATTAGATTCT+TGG - chr8.1:540900-540919 None:intergenic 20.0%
!! CCATTTCTTAAAAGTTATAA+CGG + chr8.1:541454-541473 MS.gene012767:CDS 20.0%
!! CTCTAGTTATTTAAGTATTA+AGG - chr8.1:541344-541363 None:intergenic 20.0%
!! GGTAGATATTAACAATAAAT+GGG - chr8.1:541681-541700 None:intergenic 20.0%
!! GTAGATATTAACAATAAATG+GGG - chr8.1:541680-541699 None:intergenic 20.0%
!! TCCAAAAAATAAAATGCAAA+AGG - chr8.1:541308-541327 None:intergenic 20.0%
!! TTGACATTAATAATGATGTT+GGG + chr8.1:540263-540282 MS.gene012767:CDS 20.0%
!! TTTGAACTAACAAATTTACT+AGG + chr8.1:541196-541215 MS.gene012767:intron 20.0%
!! TTTGACATTAATAATGATGT+TGG + chr8.1:540262-540281 MS.gene012767:CDS 20.0%
!!! CTAATTTTTGTTATGTTTAG+TGG + chr8.1:540905-540924 MS.gene012767:intron 20.0%
!!! TTGATAATAATTTGAGAAAC+TGG + chr8.1:541015-541034 MS.gene012767:intron 20.0%
!!! TTGTCAATTATTTTAAGTCT+TGG + chr8.1:541265-541284 MS.gene012767:intron 20.0%
! AAAAGAAACAACATCTCATT+GGG - chr8.1:540485-540504 None:intergenic 25.0%
! AATATCAAGTATTCATGTGA+AGG + chr8.1:540809-540828 MS.gene012767:intron 25.0%
! ACTTAAATAACTAGAGTTTC+TGG + chr8.1:541348-541367 MS.gene012767:intron 25.0%
! ATTTATAGCAATGCATTTGA+TGG + chr8.1:541790-541809 MS.gene012767:CDS 25.0%
! GGGTAGATATTAACAATAAA+TGG - chr8.1:541682-541701 None:intergenic 25.0%
! GTAATTTAAAAAGACCTCTA+AGG - chr8.1:541410-541429 None:intergenic 25.0%
! TGGTTTGAGTATTAATAATG+TGG + chr8.1:542209-542228 MS.gene012767:CDS 25.0%
!! TAGAGGTCTTTTTAAATTAC+AGG + chr8.1:541410-541429 MS.gene012767:intron 25.0%
!!! CCGTTATAACTTTTAAGAAA+TGG - chr8.1:541457-541476 None:intergenic 25.0%
!!! GCCTTTTGCATTTTATTTTT+TGG + chr8.1:541304-541323 MS.gene012767:intron 25.0%
CAAAAGAAACAACATCTCAT+TGG - chr8.1:540486-540505 None:intergenic 30.0%
CCAATTTCTCCAATAACTAT+AGG - chr8.1:541868-541887 None:intergenic 30.0%
CCTATAGTTATTGGAGAAAT+TGG + chr8.1:541865-541884 MS.gene012767:CDS 30.0%
GATCTCAAATAGGATATAGT+TGG + chr8.1:542478-542497 MS.gene012767:CDS 30.0%
GATTGTACAACTCTTGATTA+TGG + chr8.1:542264-542283 MS.gene012767:CDS 30.0%
GGTCAAATTCCTATAGTTAT+TGG + chr8.1:541856-541875 MS.gene012767:CDS 30.0%
TACAAGTCTTTAAACCTTAG+AGG + chr8.1:541393-541412 MS.gene012767:intron 30.0%
TAGTTATTGGAGAAATTGGT+TGG + chr8.1:541869-541888 MS.gene012767:CDS 30.0%
TGGAATAACTGAATACAACT+AGG + chr8.1:541035-541054 MS.gene012767:intron 30.0%
TTCAAAATCGTTCAGATGTA+TGG - chr8.1:540684-540703 None:intergenic 30.0%
! CTCGAAATTTCATGTCTTTT+TGG + chr8.1:540931-540950 MS.gene012767:intron 30.0%
!! GAACTAACACTTTTGATTGA+AGG - chr8.1:541072-541091 None:intergenic 30.0%
!!! AGGTAGTTTTGAAAGACATT+GGG + chr8.1:542059-542078 MS.gene012767:CDS 30.0%
AACAGATAGATATCCATAGG+AGG - chr8.1:542003-542022 None:intergenic 35.0%
AACTAGCTGAGTCATGATTT+GGG - chr8.1:541635-541654 None:intergenic 35.0%
AATCCTCAATTCCTTCACAA+GGG + chr8.1:541590-541609 MS.gene012767:CDS 35.0%
AATTACAGGTATGTTGCTGT+TGG + chr8.1:541424-541443 MS.gene012767:intron 35.0%
AGAATGCAAAAGAGTTCAGT+TGG - chr8.1:541168-541187 None:intergenic 35.0%
AGCCCAAGTAGTCATATATA+TGG - chr8.1:541222-541241 None:intergenic 35.0%
AGGCCATATATATGACTACT+TGG + chr8.1:541216-541235 MS.gene012767:intron 35.0%
ATATTTGAACCATCTGTGAC+AGG - chr8.1:541772-541791 None:intergenic 35.0%
CAATGTCTTTCAAAACTACC+TGG - chr8.1:542060-542079 None:intergenic 35.0%
CTCAAATAGGATATAGTTGG+TGG + chr8.1:542481-542500 MS.gene012767:CDS 35.0%
CTGAACAGATAGATATCCAT+AGG - chr8.1:542006-542025 None:intergenic 35.0%
GATCTATGAAACCCTTAACA+TGG + chr8.1:540963-540982 MS.gene012767:intron 35.0%
GCACTCAGTAAAATTGGATA+TGG + chr8.1:541835-541854 MS.gene012767:CDS 35.0%
GCTAAGTATCCATTAAACCT+TGG + chr8.1:542105-542124 MS.gene012767:CDS 35.0%
GCTGATCTTCTGATTATTAC+TGG + chr8.1:540859-540878 MS.gene012767:intron 35.0%
GGCCATATATATGACTACTT+GGG + chr8.1:541217-541236 MS.gene012767:intron 35.0%
TAAACTTTCAGAACAGTTGC+AGG - chr8.1:540710-540729 None:intergenic 35.0%
TAATGATGTTGGGTTTATGC+TGG + chr8.1:540273-540292 MS.gene012767:CDS 35.0%
TGTACAACTCTTGATTATGG+AGG + chr8.1:542267-542286 MS.gene012767:CDS 35.0%
TTCAGTATCTTCCGTCTAAA+TGG + chr8.1:542163-542182 MS.gene012767:CDS 35.0%
! AACCATGTTCTTGGTAACAA+GGG + chr8.1:541955-541974 MS.gene012767:CDS 35.0%
! GAACTTTTCCTTTTGAACTC+TGG + chr8.1:540831-540850 MS.gene012767:intron 35.0%
! GCAGCTAAAGTTTTCAATCA+AGG + chr8.1:541925-541944 MS.gene012767:CDS 35.0%
! TCAAGAAGCTGCAATTTTGA+TGG + chr8.1:542366-542385 MS.gene012767:CDS 35.0%
! TCTTCAATTCCTTGTTTCCA+AGG - chr8.1:542131-542150 None:intergenic 35.0%
! TGACTACTTGGGCTTTTAAT+TGG + chr8.1:541228-541247 MS.gene012767:intron 35.0%
! TGGGGGATATTTTACTTTGA+TGG + chr8.1:542078-542097 MS.gene012767:CDS 35.0%
!!! CAATTTTGATGGACTTGGTA+TGG + chr8.1:542377-542396 MS.gene012767:CDS 35.0%
!!! CAGGTAGTTTTGAAAGACAT+TGG + chr8.1:542058-542077 MS.gene012767:CDS 35.0%
!!! GGTAGTTTTGAAAGACATTG+GGG + chr8.1:542060-542079 MS.gene012767:CDS 35.0%
!!! GTAGTTTTGAAAGACATTGG+GGG + chr8.1:542061-542080 MS.gene012767:CDS 35.0%
AATCAGCCATAGGAGTAAAC+TGG + chr8.1:540312-540331 MS.gene012767:CDS 40.0%
ACAAGACTATGCGTTCTTTG+AGG + chr8.1:541735-541754 MS.gene012767:CDS 40.0%
AGCTAAAGCCAGAGTTCAAA+AGG - chr8.1:540842-540861 None:intergenic 40.0%
AGGGTTTCATAGATCAGACA+TGG - chr8.1:540958-540977 None:intergenic 40.0%
AGGTTCATGCAATGGAATTG+GGG + chr8.1:542287-542306 MS.gene012767:CDS 40.0%
ATCAGCCATAGGAGTAAACT+GGG + chr8.1:540313-540332 MS.gene012767:CDS 40.0%
ATCCACAAATCAGAAGCAGA+AGG - chr8.1:540518-540537 None:intergenic 40.0%
CAAGACTATGCGTTCTTTGA+GGG + chr8.1:541736-541755 MS.gene012767:CDS 40.0%
CCTACAACAATCCTTAGAGA+AGG + chr8.1:541513-541532 MS.gene012767:CDS 40.0%
CGTTCAGATGTATGGAAACT+AGG - chr8.1:540676-540695 None:intergenic 40.0%
CTTACCACACACCATTTAGA+CGG - chr8.1:542177-542196 None:intergenic 40.0%
GAACTAGCTGAGTCATGATT+TGG - chr8.1:541636-541655 None:intergenic 40.0%
GAATCCTCAATTCCTTCACA+AGG + chr8.1:541589-541608 MS.gene012767:CDS 40.0%
GAGGTTCATGCAATGGAATT+GGG + chr8.1:542286-542305 MS.gene012767:CDS 40.0%
GATAAAGCTACACGTTCAAC+TGG + chr8.1:542453-542472 MS.gene012767:CDS 40.0%
GATGTATGGAAACTAGGCTT+TGG - chr8.1:540670-540689 None:intergenic 40.0%
GATTATGGAGGTTCATGCAA+TGG + chr8.1:542279-542298 MS.gene012767:CDS 40.0%
GTTCAGTTCCTCAACTCAAA+CGG + chr8.1:541652-541671 MS.gene012767:CDS 40.0%
TAACGGTCAATTTCAGAACC+TGG + chr8.1:541471-541490 MS.gene012767:CDS 40.0%
TATCCATTAAACCTTGGCCT+TGG + chr8.1:542111-542130 MS.gene012767:CDS 40.0%
TATCCCTTGTGAAGGAATTG+AGG - chr8.1:541596-541615 None:intergenic 40.0%
TCATAGACATCTGCATTGCA+TGG - chr8.1:541571-541590 None:intergenic 40.0%
TCCACAAATCAGAAGCAGAA+GGG - chr8.1:540517-540536 None:intergenic 40.0%
TCTTTGCTCCTTCATCAAGA+AGG - chr8.1:542029-542048 None:intergenic 40.0%
TTTCCAAGGCCAAGGTTTAA+TGG - chr8.1:542117-542136 None:intergenic 40.0%
! ACCATGTTCTTGGTAACAAG+GGG + chr8.1:541956-541975 MS.gene012767:CDS 40.0%
! AGTGAAACTGTTTGAAGCAG+AGG + chr8.1:540406-540425 MS.gene012767:CDS 40.0%
! ATGGCTGATTCTGTCAATAC+AGG - chr8.1:540302-540321 None:intergenic 40.0%
! CAACCATGTTCTTGGTAACA+AGG + chr8.1:541954-541973 MS.gene012767:CDS 40.0%
! CCTTCTCTAAGGATTGTTGT+AGG - chr8.1:541516-541535 None:intergenic 40.0%
! GATGTTCTTAAGGCACTCAT+GGG + chr8.1:540431-540450 MS.gene012767:CDS 40.0%
! GGTCTTATCAACCATGTTCT+TGG + chr8.1:541946-541965 MS.gene012767:CDS 40.0%
! GTATTGACAGAATCAGCCAT+AGG + chr8.1:540302-540321 MS.gene012767:CDS 40.0%
! GTCATGATTTGGGTTAGATC+AGG - chr8.1:541625-541644 None:intergenic 40.0%
! TCAAAGAACGCATAGTCTTG+TGG - chr8.1:541736-541755 None:intergenic 40.0%
! TCAACTGGTGGATCTCAAAT+AGG + chr8.1:542468-542487 MS.gene012767:CDS 40.0%
! TGATGTTCTTAAGGCACTCA+TGG + chr8.1:540430-540449 MS.gene012767:CDS 40.0%
! TGATTTGGGTTAGATCAGGA+CGG - chr8.1:541621-541640 None:intergenic 40.0%
!! ATATCCATAGGAGGAGCATT+AGG - chr8.1:541994-542013 None:intergenic 40.0%
!! CAAAGAACGCATAGTCTTGT+GGG - chr8.1:541735-541754 None:intergenic 40.0%
!! GTGGTAAGTTCTAAGTCAAG+TGG + chr8.1:542189-542208 MS.gene012767:CDS 40.0%
!!! AGCTGCAATTTTGATGGACT+TGG + chr8.1:542372-542391 MS.gene012767:CDS 40.0%
!!! CTAGGCTTTGGTTTTGAAAG+AGG - chr8.1:540658-540677 None:intergenic 40.0%
AAAGCTACACGTTCAACTGG+TGG + chr8.1:542456-542475 MS.gene012767:CDS 45.0%
AAGAGATCAACCACAGTCGA+AGG - chr8.1:540365-540384 None:intergenic 45.0%
ACTACTCATCCTGTCACAGA+TGG + chr8.1:541760-541779 MS.gene012767:CDS 45.0%
ACTCATGGGAAGTGGAATTC+AGG + chr8.1:540445-540464 MS.gene012767:CDS 45.0%
AGCAGAGGCTGATGTTCTTA+AGG + chr8.1:540421-540440 MS.gene012767:CDS 45.0%
ATCCTTAGAGAAGGCAAAGC+TGG + chr8.1:541522-541541 MS.gene012767:CDS 45.0%
CAAATTGAAGCCTTCGACTG+TGG + chr8.1:540352-540371 MS.gene012767:CDS 45.0%
CAGTCGAAGGCTTCAATTTG+CGG - chr8.1:540352-540371 None:intergenic 45.0%
CCACAAATCAGAAGCAGAAG+GGG - chr8.1:540516-540535 None:intergenic 45.0%
CTGTTCAGCCTTCTTGATGA+AGG + chr8.1:542018-542037 MS.gene012767:CDS 45.0%
GAAACCCTTAACATGGACAC+CGG + chr8.1:540970-540989 MS.gene012767:intron 45.0%
GGAAATCAGTATCGTCGTAG+AGG - chr8.1:541714-541733 None:intergenic 45.0%
GGAGGTTCATGCAATGGAAT+TGG + chr8.1:542285-542304 MS.gene012767:CDS 45.0%
GTCGCAGCACTCAGTAAAAT+TGG + chr8.1:541829-541848 MS.gene012767:CDS 45.0%
TCAGCCATAGGAGTAAACTG+GGG + chr8.1:540314-540333 MS.gene012767:CDS 45.0%
TCCCCTTGTTACCAAGAACA+TGG - chr8.1:541960-541979 None:intergenic 45.0%
TCTTCCGTCTAAATGGTGTG+TGG + chr8.1:542170-542189 MS.gene012767:CDS 45.0%
TGCAATGGAATTGGGGACAA+AGG + chr8.1:542294-542313 MS.gene012767:CDS 45.0%
! AGTGGAATTCAGGTGATGGT+TGG + chr8.1:540455-540474 MS.gene012767:CDS 45.0%
! AGTTCAGTTGGTGCAGTACT+TGG - chr8.1:541156-541175 None:intergenic 45.0%
! ATTCCTTGTTTCCAAGGCCA+AGG - chr8.1:542125-542144 None:intergenic 45.0%
! GCATGATAACGAAGTACAGC+AGG - chr8.1:541116-541135 None:intergenic 45.0%
! GTGGAATTCAGGTGATGGTT+GGG + chr8.1:540456-540475 MS.gene012767:CDS 45.0%
! TGTTGTAGGTTGAGTACTGC+AGG - chr8.1:541502-541521 None:intergenic 45.0%
!! AAACCTTGGCCTTGGAAACA+AGG + chr8.1:542119-542138 MS.gene012767:CDS 45.0%
!! GAAATTGGTTGGCCTTCAGA+TGG + chr8.1:541880-541899 MS.gene012767:CDS 45.0%
!! GGAGCAAAGAGTATTCTTCC+AGG + chr8.1:542039-542058 MS.gene012767:CDS 45.0%
ACAGAGCCTTGCAACAGGTT+TGG + chr8.1:540227-540246 MS.gene012767:CDS 50.0%
ACTTCCAGAGACCCTTCAGT+CGG + chr8.1:542402-542421 MS.gene012767:CDS 50.0%
AGAACGTCTCTGCTTACACC+GGG + chr8.1:540543-540562 MS.gene012767:CDS 50.0%
AGCCAGCTTTGCCTTCTCTA+AGG - chr8.1:541527-541546 None:intergenic 50.0%
CAAATCAGAAGCAGAAGGGG+AGG - chr8.1:540513-540532 None:intergenic 50.0%
CAGAACGTCTCTGCTTACAC+CGG + chr8.1:540542-540561 MS.gene012767:CDS 50.0%
CATAGGAGTAAACTGGGGCA+CGG + chr8.1:540319-540338 MS.gene012767:CDS 50.0%
CTTAACATGGACACCGGAGA+CGG + chr8.1:540976-540995 MS.gene012767:intron 50.0%
GATATCAGCACCACCTTTCC+CGG - chr8.1:540564-540583 None:intergenic 50.0%
GCACCAATTGCTCCATCTGA+AGG - chr8.1:541895-541914 None:intergenic 50.0%
GCCATACCAAACCTGTTGCA+AGG - chr8.1:540236-540255 None:intergenic 50.0%
GGACGGAATATCCCTTGTGA+AGG - chr8.1:541604-541623 None:intergenic 50.0%
GGGAAGTGGAATTCAGGTGA+TGG + chr8.1:540451-540470 MS.gene012767:CDS 50.0%
GTAACAAGGGGACACCTTTG+AGG + chr8.1:541968-541987 MS.gene012767:CDS 50.0%
GTCTCCGGTGTCCATGTTAA+GGG - chr8.1:540977-540996 None:intergenic 50.0%
TGGCCTTCAGATGGAGCAAT+TGG + chr8.1:541889-541908 MS.gene012767:CDS 50.0%
! CCCCTTCTGCTTCTGATTTG+TGG + chr8.1:540513-540532 MS.gene012767:CDS 50.0%
! CTTAAGGCACTCATGGGAAG+TGG + chr8.1:540437-540456 MS.gene012767:CDS 50.0%
! GCCTTGCAACAGGTTTGGTA+TGG + chr8.1:540232-540251 MS.gene012767:CDS 50.0%
!! ATGGGGAACCGTTTGAGTTG+AGG - chr8.1:541663-541682 None:intergenic 50.0%
!! GGAAAGGTGGTGCTGATATC+AGG + chr8.1:540564-540583 MS.gene012767:CDS 50.0%
!!! GGCGACTGTCGTTTTATCGT+TGG + chr8.1:542423-542442 MS.gene012767:CDS 50.0%
CAGTATCGTCGTAGAGGCTG+AGG - chr8.1:541708-541727 None:intergenic 55.0%
CGGTGTAAGCAGAGACGTTC+TGG - chr8.1:540544-540563 None:intergenic 55.0%
CGTCGTAGAGGCTGAGGAAA+GGG - chr8.1:541702-541721 None:intergenic 55.0%
CGTCTCCGGTGTCCATGTTA+AGG - chr8.1:540978-540997 None:intergenic 55.0%
CGTGCCCCAGTTTACTCCTA+TGG - chr8.1:540321-540340 None:intergenic 55.0%
GAGGCCTAATGCTCCTCCTA+TGG + chr8.1:541987-542006 MS.gene012767:CDS 55.0%
GTCTCTGCTTACACCGGGAA+AGG + chr8.1:540548-540567 MS.gene012767:CDS 55.0%
TCGTCGTAGAGGCTGAGGAA+AGG - chr8.1:541703-541722 None:intergenic 55.0%
TCTGCTTACACCGGGAAAGG+TGG + chr8.1:540551-540570 MS.gene012767:CDS 55.0%
! AAACGACAGTCGCCGACTGA+AGG - chr8.1:542417-542436 None:intergenic 55.0%
! AACGACAGTCGCCGACTGAA+GGG - chr8.1:542416-542435 None:intergenic 55.0%
! CGATGTGTCATTGCCGTCTC+CGG - chr8.1:540992-541011 None:intergenic 55.0%
! TGAGTACTGCAGGCATGACC+AGG - chr8.1:541492-541511 None:intergenic 55.0%
!! GGAGGAGCATTAGGCCTCAA+AGG - chr8.1:541985-542004 None:intergenic 55.0%
! GTCGCCGACTGAAGGGTCTC+TGG - chr8.1:542409-542428 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 540224 542560 540224 ID=MS.gene012767
chr8.1 mRNA 540224 542560 540224 ID=MS.gene012767.t1;Parent=MS.gene012767
chr8.1 exon 540224 540585 540224 ID=MS.gene012767.t1.exon1;Parent=MS.gene012767.t1
chr8.1 CDS 540224 540585 540224 ID=cds.MS.gene012767.t1;Parent=MS.gene012767.t1
chr8.1 exon 541432 542560 541432 ID=MS.gene012767.t1.exon2;Parent=MS.gene012767.t1
chr8.1 CDS 541432 542560 541432 ID=cds.MS.gene012767.t1;Parent=MS.gene012767.t1
Gene Sequence

>MS.gene012767

ATGACAGAGCCTTGCAACAGGTTTGGTATGGCTAGTATTTTGACATTAATAATGATGTTGGGTTTATGCTGGATTCCTGTATTGACAGAATCAGCCATAGGAGTAAACTGGGGCACGGTTTCATTCCGCAAATTGAAGCCTTCGACTGTGGTTGATCTCTTAATAGACAACAAAATCACAAAAGTGAAACTGTTTGAAGCAGAGGCTGATGTTCTTAAGGCACTCATGGGAAGTGGAATTCAGGTGATGGTTGGGATTCCCAATGAGATGTTGTTTCTTTTGAGTTCCTCCCCTTCTGCTTCTGATTTGTGGATTCGCCAGAACGTCTCTGCTTACACCGGGAAAGGTGGTGCTGATATCAGGTTAGTAACATGTCATCTAACTTTATTTGTACGTTATCACTGTATTATTCTTCTATCTTTTTATTCGATCCTCTTTCAAAACCAAAGCCTAGTTTCCATACATCTGAACGATTTTGAATAACCTGCAACTGTTCTGAAAGTTTAAATTTCGACATCTTTTACAACAAATAATAAGGATATTTATTGAGTTAGCTTGAAGAACATTGTTTTCATGTTTAAGTAAAATATCAAGTATTCATGTGAAGGAACTTTTCCTTTTGAACTCTGGCTTTAGCTGATCTTCTGATTATTACTGGATAGTATCTCTATAACCAAGAATCTAATTTTTGTTATGTTTAGTGGAAGCTCGAAATTTCATGTCTTTTTGGTCCATGTCTGATCTATGAAACCCTTAACATGGACACCGGAGACGGCAATGACACATCGACATTGATAATAATTTGAGAAACTGGAATAACTGAATACAACTAGGTGTATCATATGCCTTCAATCAAAAGTGTTAGTTCGACAAAGCTTTTGACGTCTATCCTGCTGTACTTCGTTATCATGCTGATGAATAGTGTCATACCAAGTACTGCACCAACTGAACTCTTTTGCATTCTATATGTAATTTGAACTAACAAATTTACTAGGCCATATATATGACTACTTGGGCTTTTAATTGGATAATTAAATATTCTTGTCAATTATTTTAAGTCTTGGTGTGAATAACTCAGATGCCTTTTGCATTTTATTTTTTGGATGTTGTTATTGTGCCTTAATACTTAAATAACTAGAGTTTCTGGAAGTATGTACAAGTTGATTTTCTACAAGTCTTTAAACCTTAGAGGTCTTTTTAAATTACAGGTATGTTGCTGTTGGAAATGAACCATTTCTTAAAAGTTATAACGGTCAATTTCAGAACCTGGTCATGCCTGCAGTACTCAACCTACAACAATCCTTAGAGAAGGCAAAGCTGGCTAGTTCAATAAAACTAGTTGTTCCATGCAATGCAGATGTCTATGAATCCTCAATTCCTTCACAAGGGATATTCCGTCCTGATCTAACCCAAATCATGACTCAGCTAGTTCAGTTCCTCAACTCAAACGGTTCCCCATTTATTGTTAATATCTACCCTTTCCTCAGCCTCTACGACGATACTGATTTCCCACAAGACTATGCGTTCTTTGAGGGAACTACTCATCCTGTCACAGATGGTTCAAATATTTATAGCAATGCATTTGATGGAAACTATGACACTTTAGTCGCAGCACTCAGTAAAATTGGATATGGTCAAATTCCTATAGTTATTGGAGAAATTGGTTGGCCTTCAGATGGAGCAATTGGTGCAAACATCACTGCAGCTAAAGTTTTCAATCAAGGTCTTATCAACCATGTTCTTGGTAACAAGGGGACACCTTTGAGGCCTAATGCTCCTCCTATGGATATCTATCTGTTCAGCCTTCTTGATGAAGGAGCAAAGAGTATTCTTCCAGGTAGTTTTGAAAGACATTGGGGGATATTTTACTTTGATGGAAAAGCTAAGTATCCATTAAACCTTGGCCTTGGAAACAAGGAATTGAAGAACGCAAAGAATGTTCAGTATCTTCCGTCTAAATGGTGTGTGGTAAGTTCTAAGTCAAGTGGTTTGAGTATTAATAATGTGGCAAACTATATGAGAACTGCATGCAGTGTTGCAGATTGTACAACTCTTGATTATGGAGGTTCATGCAATGGAATTGGGGACAAAGGTAATATATCATATGCTTTTAACAGTTACTATCAACTAAAAATGCAAGACTCAAGAAGCTGCAATTTTGATGGACTTGGTATGGTTACTTCCAGAGACCCTTCAGTCGGCGACTGTCGTTTTATCGTTGGAATTACAGATAAAGCTACACGTTCAACTGGTGGATCTCAAATAGGATATAGTTGGTGGTTTCTATTTCTAGTAACATTTTCAATTATAGAAATAACTTTTTCACTTTCAATGTGA
Protein sequence

>MS.gene012767.t1

MTEPCNRFGMASILTLIMMLGLCWIPVLTESAIGVNWGTVSFRKLKPSTVVDLLIDNKITKVKLFEAEADVLKALMGSGIQVMVGIPNEMLFLLSSSPSASDLWIRQNVSAYTGKGGADIRYVAVGNEPFLKSYNGQFQNLVMPAVLNLQQSLEKAKLASSIKLVVPCNADVYESSIPSQGIFRPDLTQIMTQLVQFLNSNGSPFIVNIYPFLSLYDDTDFPQDYAFFEGTTHPVTDGSNIYSNAFDGNYDTLVAALSKIGYGQIPIVIGEIGWPSDGAIGANITAAKVFNQGLINHVLGNKGTPLRPNAPPMDIYLFSLLDEGAKSILPGSFERHWGIFYFDGKAKYPLNLGLGNKELKNAKNVQYLPSKWCVVSSKSSGLSINNVANYMRTACSVADCTTLDYGGSCNGIGDKGNISYAFNSYYQLKMQDSRSCNFDGLGMVTSRDPSVGDCRFIVGITDKATRSTGGSQIGYSWWFLFLVTFSIIEITFSLSM