Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013462.t1 | XP_003602442.1 | 99.1 | 110 | 1 | 0 | 1 | 110 | 8 | 117 | 4.10E-36 | 160.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013462.t1 | Q9FIR9 | 44.1 | 102 | 48 | 2 | 5 | 106 | 1 | 93 | 3.3e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013462.t1 | G7J9Q1 | 99.1 | 110 | 1 | 0 | 1 | 110 | 8 | 117 | 3.0e-36 | 160.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene049438 | MS.gene013462 | PPI |
| MS.gene013462 | MS.gene06567 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013462.t1 | MTR_3g093400 | 99.091 | 110 | 1 | 0 | 1 | 110 | 8 | 117 | 1.92e-74 | 216 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013462.t1 | AT5G24660 | 49.495 | 99 | 35 | 3 | 5 | 100 | 1 | 87 | 7.99e-23 | 85.1 |
| MS.gene013462.t1 | AT5G24655 | 48.485 | 99 | 36 | 3 | 5 | 100 | 1 | 87 | 3.48e-21 | 80.9 |
| MS.gene013462.t1 | AT3G49580 | 54.667 | 75 | 27 | 1 | 23 | 97 | 17 | 84 | 3.04e-20 | 78.6 |
| MS.gene013462.t1 | AT3G49570 | 53.125 | 96 | 36 | 2 | 5 | 100 | 1 | 87 | 1.65e-19 | 76.6 |
| MS.gene013462.t1 | AT3G49580 | 73.684 | 38 | 10 | 0 | 60 | 97 | 28 | 65 | 2.72e-13 | 60.5 |
Find 22 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGCGCGTGGCGGAGGAAG+CGG | 0.401471 | 3.4:-85264501 | MS.gene013462:CDS |
| GGAGAGGCTGCGCGTGGCGG+AGG | 0.410992 | 3.4:-85264507 | MS.gene013462:CDS |
| CTTCTCTTCAGCTCCGACTC+CGG | 0.438820 | 3.4:+85264608 | None:intergenic |
| CGATCGTATTGTTTCTCTGA+TGG | 0.452561 | 3.4:-85264411 | MS.gene013462:CDS |
| TCAGCTTGAGAAAGAGTTGA+AGG | 0.470244 | 3.4:-85264579 | MS.gene013462:CDS |
| ATGCTCACAGCTCGGAGAGC+TGG | 0.481100 | 3.4:-85264468 | MS.gene013462:CDS |
| AGTTACAGAGCGCGTGGGAG+AGG | 0.507673 | 3.4:-85264523 | MS.gene013462:CDS |
| GAAAGAGTTGAAGGAGAGCA+AGG | 0.510690 | 3.4:-85264570 | MS.gene013462:CDS |
| GTGGGAGAGGCTGCGCGTGG+CGG | 0.510805 | 3.4:-85264510 | MS.gene013462:CDS |
| TGCGGTGCCGGTGCCGGAGT+CGG | 0.513302 | 3.4:-85264621 | MS.gene013462:CDS |
| GTGCCGATCACTGCCATCAT+CGG | 0.537295 | 3.4:+85264668 | None:intergenic |
| TTCAGCTCCGACTCCGGCAC+CGG | 0.539404 | 3.4:+85264614 | None:intergenic |
| TCACCGATGATGGCAGTGAT+CGG | 0.571734 | 3.4:-85264671 | None:intergenic |
| CGCGTGGGAGAGGCTGCGCG+TGG | 0.573568 | 3.4:-85264513 | MS.gene013462:CDS |
| CAAAACCAATCACAACGAGT+TGG | 0.575914 | 3.4:+85264346 | None:intergenic |
| ATGATGGCAGTGATCGGCAC+CGG | 0.580016 | 3.4:-85264665 | MS.gene013462:CDS |
| GCGCGTGGCGGAGGAAGCGG+AGG | 0.588927 | 3.4:-85264498 | MS.gene013462:CDS |
| GACGGCAGTTACAGAGCGCG+TGG | 0.606840 | 3.4:-85264529 | MS.gene013462:CDS |
| AGAGAGAAGAGCAAATGAGA+CGG | 0.606848 | 3.4:-85264547 | MS.gene013462:CDS |
| ATGGCAGTGATCGGCACCGG+AGG | 0.608782 | 3.4:-85264662 | MS.gene013462:CDS |
| GAGAGATTATGCTCACAGCT+CGG | 0.617852 | 3.4:-85264476 | MS.gene013462:CDS |
| ACGGCAGTTACAGAGCGCGT+GGG | 0.696408 | 3.4:-85264528 | MS.gene013462:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAGAGTCTAAAAATG+CGG | - | chr3.4:85264381-85264400 | MS.gene013462:CDS | 20.0% |
| !!! | TTTAGACTCTTTTTTTCCTC+CGG | + | chr3.4:85264377-85264396 | None:intergenic | 30.0% |
| AGAGAGAAGAGCAAATGAGA+CGG | - | chr3.4:85264473-85264492 | MS.gene013462:CDS | 40.0% | |
| TCAGCTTGAGAAAGAGTTGA+AGG | - | chr3.4:85264441-85264460 | MS.gene013462:CDS | 40.0% | |
| ! | CGATCGTATTGTTTCTCTGA+TGG | - | chr3.4:85264609-85264628 | MS.gene013462:CDS | 40.0% |
| AGAGTCTAAAAATGCGGTGC+CGG | - | chr3.4:85264387-85264406 | MS.gene013462:CDS | 45.0% | |
| GAAAGAGTTGAAGGAGAGCA+AGG | - | chr3.4:85264450-85264469 | MS.gene013462:CDS | 45.0% | |
| GAGAGATTATGCTCACAGCT+CGG | - | chr3.4:85264544-85264563 | MS.gene013462:CDS | 45.0% | |
| ATGATGGCAGTGATCGGCAC+CGG | - | chr3.4:85264355-85264374 | MS.gene013462:CDS | 55.0% | |
| CTTCTCTTCAGCTCCGACTC+CGG | + | chr3.4:85264415-85264434 | None:intergenic | 55.0% | |
| !! | TAAAAATGCGGTGCCGGTGC+CGG | - | chr3.4:85264393-85264412 | MS.gene013462:CDS | 55.0% |
| ACGGCAGTTACAGAGCGCGT+GGG | - | chr3.4:85264492-85264511 | MS.gene013462:CDS | 60.0% | |
| AGTTACAGAGCGCGTGGGAG+AGG | - | chr3.4:85264497-85264516 | MS.gene013462:CDS | 60.0% | |
| ATGCTCACAGCTCGGAGAGC+TGG | - | chr3.4:85264552-85264571 | MS.gene013462:CDS | 60.0% | |
| GACGGCAGTTACAGAGCGCG+TGG | - | chr3.4:85264491-85264510 | MS.gene013462:CDS | 65.0% | |
| TTCAGCTCCGACTCCGGCAC+CGG | + | chr3.4:85264409-85264428 | None:intergenic | 65.0% | |
| ! | ATGGCAGTGATCGGCACCGG+AGG | - | chr3.4:85264358-85264377 | MS.gene013462:CDS | 65.0% |
| GTGGGAGAGGCTGCGCGTGG+CGG | - | chr3.4:85264510-85264529 | MS.gene013462:CDS | 75.0% | |
| ! | GCTGCGCGTGGCGGAGGAAG+CGG | - | chr3.4:85264519-85264538 | MS.gene013462:CDS | 75.0% |
| !! | TGCGGTGCCGGTGCCGGAGT+CGG | - | chr3.4:85264399-85264418 | MS.gene013462:CDS | 75.0% |
| ! | CGCGTGGGAGAGGCTGCGCG+TGG | - | chr3.4:85264507-85264526 | MS.gene013462:CDS | 80.0% |
| ! | GGAGAGGCTGCGCGTGGCGG+AGG | - | chr3.4:85264513-85264532 | MS.gene013462:CDS | 80.0% |
| !! | GCGCGTGGCGGAGGAAGCGG+AGG | - | chr3.4:85264522-85264541 | MS.gene013462:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 85264355 | 85264687 | 85264355 | ID=MS.gene013462 |
| chr3.4 | mRNA | 85264355 | 85264687 | 85264355 | ID=MS.gene013462.t1;Parent=MS.gene013462 |
| chr3.4 | exon | 85264355 | 85264687 | 85264355 | ID=MS.gene013462.t1.exon1;Parent=MS.gene013462.t1 |
| chr3.4 | CDS | 85264355 | 85264687 | 85264355 | ID=cds.MS.gene013462.t1;Parent=MS.gene013462.t1 |
| Gene Sequence |
| Protein sequence |
