Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06567.t1 | XP_003602442.1 | 94.5 | 110 | 6 | 0 | 1 | 110 | 8 | 117 | 2.70E-35 | 157.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06567.t1 | Q9FIR9 | 46.1 | 102 | 46 | 2 | 5 | 106 | 1 | 93 | 3.5e-12 | 72.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06567.t1 | G7J9Q1 | 94.5 | 110 | 6 | 0 | 1 | 110 | 8 | 117 | 1.9e-35 | 157.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049438 | MS.gene06567 | 0.828422 | 9.07E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene049438 | MS.gene06567 | PPI |
| MS.gene013462 | MS.gene06567 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06567.t1 | MTR_3g093400 | 96.364 | 110 | 4 | 0 | 1 | 110 | 8 | 117 | 2.43e-72 | 211 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06567.t1 | AT5G24660 | 49.495 | 99 | 35 | 3 | 5 | 100 | 1 | 87 | 9.72e-23 | 85.1 |
| MS.gene06567.t1 | AT5G24655 | 48.485 | 99 | 36 | 3 | 5 | 100 | 1 | 87 | 5.16e-21 | 80.5 |
| MS.gene06567.t1 | AT3G49580 | 54.667 | 75 | 27 | 1 | 23 | 97 | 17 | 84 | 2.58e-20 | 79.0 |
| MS.gene06567.t1 | AT3G49570 | 52.083 | 96 | 37 | 2 | 5 | 100 | 1 | 87 | 2.47e-19 | 76.3 |
| MS.gene06567.t1 | AT3G49580 | 73.684 | 38 | 10 | 0 | 60 | 97 | 28 | 65 | 2.87e-13 | 60.5 |
Find 24 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAGAGTTGAAGGAGAGT+AGG | 0.397427 | 3.2:-78439974 | MS.gene06567:CDS |
| GCTGCGCGTGGCGGAGGAAG+CGG | 0.401471 | 3.2:-78439904 | MS.gene06567:CDS |
| GAAAGAGTTGAAGGAGAGTA+GGG | 0.413600 | 3.2:-78439973 | MS.gene06567:CDS |
| CTTCTCTTCAGCTCCGACTC+CGG | 0.438820 | 3.2:+78440011 | None:intergenic |
| CGATCGTATTGTTTCTCTGA+TGG | 0.452561 | 3.2:-78439814 | MS.gene06567:CDS |
| GGAAAGGCTGCGCGTGGCGG+AGG | 0.464821 | 3.2:-78439910 | MS.gene06567:CDS |
| TCAGCTTGAGAAAGAGTTGA+AGG | 0.470244 | 3.2:-78439982 | MS.gene06567:CDS |
| ATGCTCACAGCTCGGAGAGC+TGG | 0.481510 | 3.2:-78439871 | MS.gene06567:CDS |
| GTGGGAAAGGCTGCGCGTGG+CGG | 0.509515 | 3.2:-78439913 | MS.gene06567:CDS |
| TGCGGTGCCGGTGCCGGAGT+CGG | 0.513302 | 3.2:-78440024 | MS.gene06567:CDS |
| AGTTACAGAGCGCGTGGGAA+AGG | 0.524335 | 3.2:-78439926 | MS.gene06567:CDS |
| GTGCCGATCACTGCCATCAT+CGG | 0.537295 | 3.2:+78440071 | None:intergenic |
| CGCGTGGGAAAGGCTGCGCG+TGG | 0.538078 | 3.2:-78439916 | MS.gene06567:CDS |
| TTCAGCTCCGACTCCGGCAC+CGG | 0.539404 | 3.2:+78440017 | None:intergenic |
| TCACCGATGATGGCAGTGAT+CGG | 0.571734 | 3.2:-78440074 | None:intergenic |
| ATGATGGCAGTGATCGGCAC+CGG | 0.580016 | 3.2:-78440068 | MS.gene06567:CDS |
| GCGCGTGGCGGAGGAAGCGG+AGG | 0.588927 | 3.2:-78439901 | MS.gene06567:CDS |
| GACAGCAGTTACAGAGCGCG+TGG | 0.592174 | 3.2:-78439932 | MS.gene06567:CDS |
| TGGCGGAGGAAGCGGAGGAG+AGG | 0.608333 | 3.2:-78439896 | MS.gene06567:CDS |
| ATGGCAGTGATCGGCACCGG+AGG | 0.608782 | 3.2:-78440065 | MS.gene06567:CDS |
| GAGAGGTTATGCTCACAGCT+CGG | 0.627892 | 3.2:-78439879 | MS.gene06567:CDS |
| ACAGCAGTTACAGAGCGCGT+GGG | 0.694176 | 3.2:-78439931 | MS.gene06567:CDS |
| CAAAACCAATCACAACGAGG+TGG | 0.696964 | 3.2:+78439749 | None:intergenic |
| ACACAAAACCAATCACAACG+AGG | 0.794409 | 3.2:+78439746 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAGAGTCTAAAAATG+CGG | - | chr3.2:78439784-78439803 | MS.gene06567:CDS | 20.0% |
| !!! | TTTAGACTCTTTTTTTCCTC+CGG | + | chr3.2:78439780-78439799 | None:intergenic | 30.0% |
| AGAAAGAGTTGAAGGAGAGT+AGG | - | chr3.2:78439852-78439871 | MS.gene06567:CDS | 40.0% | |
| GAAAGAGTTGAAGGAGAGTA+GGG | - | chr3.2:78439853-78439872 | MS.gene06567:CDS | 40.0% | |
| TCAGCTTGAGAAAGAGTTGA+AGG | - | chr3.2:78439844-78439863 | MS.gene06567:CDS | 40.0% | |
| ! | CGATCGTATTGTTTCTCTGA+TGG | - | chr3.2:78440012-78440031 | MS.gene06567:CDS | 40.0% |
| AGAGTCTAAAAATGCGGTGC+CGG | - | chr3.2:78439790-78439809 | MS.gene06567:CDS | 45.0% | |
| GAGAGGTTATGCTCACAGCT+CGG | - | chr3.2:78439947-78439966 | MS.gene06567:CDS | 50.0% | |
| ACAGCAGTTACAGAGCGCGT+GGG | - | chr3.2:78439895-78439914 | MS.gene06567:CDS | 55.0% | |
| AGTTACAGAGCGCGTGGGAA+AGG | - | chr3.2:78439900-78439919 | MS.gene06567:CDS | 55.0% | |
| ATGATGGCAGTGATCGGCAC+CGG | - | chr3.2:78439758-78439777 | MS.gene06567:CDS | 55.0% | |
| CTTCTCTTCAGCTCCGACTC+CGG | + | chr3.2:78439818-78439837 | None:intergenic | 55.0% | |
| !! | TAAAAATGCGGTGCCGGTGC+CGG | - | chr3.2:78439796-78439815 | MS.gene06567:CDS | 55.0% |
| ATGCTCACAGCTCGGAGAGC+TGG | - | chr3.2:78439955-78439974 | MS.gene06567:CDS | 60.0% | |
| GACAGCAGTTACAGAGCGCG+TGG | - | chr3.2:78439894-78439913 | MS.gene06567:CDS | 60.0% | |
| TTCAGCTCCGACTCCGGCAC+CGG | + | chr3.2:78439812-78439831 | None:intergenic | 65.0% | |
| ! | ATGGCAGTGATCGGCACCGG+AGG | - | chr3.2:78439761-78439780 | MS.gene06567:CDS | 65.0% |
| GTGGGAAAGGCTGCGCGTGG+CGG | - | chr3.2:78439913-78439932 | MS.gene06567:CDS | 70.0% | |
| TGGCGGAGGAAGCGGAGGAG+AGG | - | chr3.2:78439930-78439949 | MS.gene06567:CDS | 70.0% | |
| CGCGTGGGAAAGGCTGCGCG+TGG | - | chr3.2:78439910-78439929 | MS.gene06567:CDS | 75.0% | |
| GGAAAGGCTGCGCGTGGCGG+AGG | - | chr3.2:78439916-78439935 | MS.gene06567:CDS | 75.0% | |
| ! | GCTGCGCGTGGCGGAGGAAG+CGG | - | chr3.2:78439922-78439941 | MS.gene06567:CDS | 75.0% |
| !! | TGCGGTGCCGGTGCCGGAGT+CGG | - | chr3.2:78439802-78439821 | MS.gene06567:CDS | 75.0% |
| !! | GCGCGTGGCGGAGGAAGCGG+AGG | - | chr3.2:78439925-78439944 | MS.gene06567:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 78439758 | 78440090 | 78439758 | ID=MS.gene06567 |
| chr3.2 | mRNA | 78439758 | 78440090 | 78439758 | ID=MS.gene06567.t1;Parent=MS.gene06567 |
| chr3.2 | exon | 78439758 | 78440090 | 78439758 | ID=MS.gene06567.t1.exon1;Parent=MS.gene06567.t1 |
| chr3.2 | CDS | 78439758 | 78440090 | 78439758 | ID=cds.MS.gene06567.t1;Parent=MS.gene06567.t1 |
| Gene Sequence |
| Protein sequence |
