AlfalfaGEDB | MS.gene049438

M. sativa cultivar XinJiangDaYe / MS.gene049438

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049438.t1	XP_003602442.1	99.1	110	1	0	1	110	8	117	4.10E-36	160.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049438.t1	Q9FIR9	44.1	102	48	2	5	106	1	93	3.3e-10	65.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049438.t1	G7J9Q1	99.1	110	1	0	1	110	8	117	3.0e-36	160.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049438	MS.gene054930	0.802661	5.05E-49	-1.69E-46
MS.gene049438	MS.gene058661	-0.820261	7.53E-53	-1.69E-46
MS.gene049438	MS.gene059872	-0.809713	1.65E-50	-1.69E-46
MS.gene049438	MS.gene06567	0.828422	9.07E-55	-1.69E-46
MS.gene049438	MS.gene06652	0.828203	1.02E-54	-1.69E-46
MS.gene049438	MS.gene067857	0.801776	7.68E-49	-1.69E-46
MS.gene049438	MS.gene068683	-0.840995	6.21E-58	-1.69E-46
MS.gene049438	MS.gene08260	-0.801945	7.09E-49	-1.69E-46
MS.gene049438	MS.gene08797	-0.804536	2.06E-49	-1.69E-46
MS.gene049438	MS.gene22314	0.805878	1.08E-49	-1.69E-46
MS.gene049438	MS.gene27108	-0.8001	1.69E-48	-1.69E-46
MS.gene049438	MS.gene39313	0.806955	6.40E-50	-1.69E-46
MS.gene049438	MS.gene40917	-0.802573	5.26E-49	-1.69E-46
MS.gene049438	MS.gene56493	0.819173	1.33E-52	-1.69E-46
MS.gene049438	MS.gene66408	0.814187	1.75E-51	-1.69E-46
MS.gene049438	MS.gene69314	0.802891	4.52E-49	-1.69E-46
MS.gene049438	MS.gene74237	0.800776	1.23E-48	-1.69E-46
MS.gene049438	MS.gene82350	0.82757	1.45E-54	-1.69E-46
MS.gene049438	MS.gene82352	0.824326	8.58E-54	-1.69E-46
MS.gene049438	MS.gene82354	0.828202	1.03E-54	-1.69E-46
MS.gene049438	MS.gene82976	0.820929	5.29E-53	-1.69E-46
MS.gene049438	MS.gene82978	0.837011	6.68E-57	-1.69E-46
MS.gene049438	MS.gene82983	0.806722	7.17E-50	-1.69E-46
MS.gene049438	MS.gene87170	0.801187	1.01E-48	-1.69E-46
MS.gene049438	MS.gene90119	0.824127	9.55E-54	-1.69E-46
MS.gene049438	MS.gene91006	0.804573	2.02E-49	-1.69E-46
MS.gene049438	MS.gene93736	-0.821896	3.16E-53	-1.69E-46
MS.gene049438	MS.gene96220	0.802131	6.49E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene049438	MS.gene06567	PPI
MS.gene049438	MS.gene013462	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049438.t1	MTR_3g093400	99.091	110	1	0	1	110	8	117	1.92e-74	216

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049438.t1	AT5G24660	49.495	99	35	3	5	100	1	87	7.99e-23	85.1
MS.gene049438.t1	AT5G24655	48.485	99	36	3	5	100	1	87	3.48e-21	80.9
MS.gene049438.t1	AT3G49580	54.667	75	27	1	23	97	17	84	3.04e-20	78.6
MS.gene049438.t1	AT3G49570	53.125	96	36	2	5	100	1	87	1.65e-19	76.6
MS.gene049438.t1	AT3G49580	73.684	38	10	0	60	97	28	65	2.72e-13	60.5

Find 23 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGCGCGTGGCGGAGGAAG+CGG	0.401471	3.3:-77815964	MS.gene049438:CDS
GGAGAGGCTGCGCGTGGCGG+AGG	0.410992	3.3:-77815970	MS.gene049438:CDS
CTTCTCTTCAGCTCCGACTC+CGG	0.438820	3.3:+77816071	None:intergenic
CGATCGTATTGTTTCTCTGA+TGG	0.452561	3.3:-77815874	MS.gene049438:CDS
TCAGCTTGAGAAAGAGTTGA+AGG	0.470244	3.3:-77816042	MS.gene049438:CDS
ATGCTCACAGCTCGGAGAGC+TGG	0.481100	3.3:-77815931	MS.gene049438:CDS
TTCAGCTCCGACTCCGGCAC+GGG	0.483732	3.3:+77816077	None:intergenic
AGTTACAGAGCGCGTGGGAG+AGG	0.507673	3.3:-77815986	MS.gene049438:CDS
GAAAGAGTTGAAGGAGAGCA+AGG	0.510690	3.3:-77816033	MS.gene049438:CDS
GTGGGAGAGGCTGCGCGTGG+CGG	0.510805	3.3:-77815973	MS.gene049438:CDS
CTTCAGCTCCGACTCCGGCA+CGG	0.512394	3.3:+77816076	None:intergenic
TGCGGTGCCCGTGCCGGAGT+CGG	0.513302	3.3:-77816084	MS.gene049438:CDS
GTGCCGATCACTGCCATCAT+CGG	0.537295	3.3:+77816131	None:intergenic
TCACCGATGATGGCAGTGAT+CGG	0.571734	3.3:-77816134	None:intergenic
CGCGTGGGAGAGGCTGCGCG+TGG	0.573568	3.3:-77815976	MS.gene049438:CDS
CAAAACCAATCACAACGAGT+TGG	0.575914	3.3:+77815809	None:intergenic
ATGATGGCAGTGATCGGCAC+CGG	0.580016	3.3:-77816128	MS.gene049438:CDS
GCGCGTGGCGGAGGAAGCGG+AGG	0.588927	3.3:-77815961	MS.gene049438:CDS
GACGGCAGTTACAGAGCGCG+TGG	0.606840	3.3:-77815992	MS.gene049438:CDS
AGAGAGAAGAGCAAATGAGA+CGG	0.606848	3.3:-77816010	MS.gene049438:CDS
ATGGCAGTGATCGGCACCGG+AGG	0.608782	3.3:-77816125	MS.gene049438:CDS
GAGAGATTATGCTCACAGCT+CGG	0.617852	3.3:-77815939	MS.gene049438:CDS
ACGGCAGTTACAGAGCGCGT+GGG	0.696408	3.3:-77815991	MS.gene049438:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAGAGTCTAAAAATG+CGG	-	chr3.3:77815844-77815863	MS.gene049438:CDS	20.0%
!!!	TTTAGACTCTTTTTTTCCTC+CGG	+	chr3.3:77815840-77815859	None:intergenic	30.0%
	AGAGAGAAGAGCAAATGAGA+CGG	-	chr3.3:77815936-77815955	MS.gene049438:CDS	40.0%
	TCAGCTTGAGAAAGAGTTGA+AGG	-	chr3.3:77815904-77815923	MS.gene049438:CDS	40.0%
!	CGATCGTATTGTTTCTCTGA+TGG	-	chr3.3:77816072-77816091	MS.gene049438:CDS	40.0%
	GAAAGAGTTGAAGGAGAGCA+AGG	-	chr3.3:77815913-77815932	MS.gene049438:CDS	45.0%
	GAGAGATTATGCTCACAGCT+CGG	-	chr3.3:77816007-77816026	MS.gene049438:CDS	45.0%
	ATGATGGCAGTGATCGGCAC+CGG	-	chr3.3:77815818-77815837	MS.gene049438:CDS	55.0%
	CTTCTCTTCAGCTCCGACTC+CGG	+	chr3.3:77815878-77815897	None:intergenic	55.0%
	TAAAAATGCGGTGCCCGTGC+CGG	-	chr3.3:77815856-77815875	MS.gene049438:CDS	55.0%
	ACGGCAGTTACAGAGCGCGT+GGG	-	chr3.3:77815955-77815974	MS.gene049438:CDS	60.0%
	AGTTACAGAGCGCGTGGGAG+AGG	-	chr3.3:77815960-77815979	MS.gene049438:CDS	60.0%
	ATGCTCACAGCTCGGAGAGC+TGG	-	chr3.3:77816015-77816034	MS.gene049438:CDS	60.0%
	CTTCAGCTCCGACTCCGGCA+CGG	+	chr3.3:77815873-77815892	None:intergenic	65.0%
	GACGGCAGTTACAGAGCGCG+TGG	-	chr3.3:77815954-77815973	MS.gene049438:CDS	65.0%
	TTCAGCTCCGACTCCGGCAC+GGG	+	chr3.3:77815872-77815891	None:intergenic	65.0%
!	ATGGCAGTGATCGGCACCGG+AGG	-	chr3.3:77815821-77815840	MS.gene049438:CDS	65.0%
	GTGGGAGAGGCTGCGCGTGG+CGG	-	chr3.3:77815973-77815992	MS.gene049438:CDS	75.0%
	TGCGGTGCCCGTGCCGGAGT+CGG	-	chr3.3:77815862-77815881	MS.gene049438:CDS	75.0%
!	GCTGCGCGTGGCGGAGGAAG+CGG	-	chr3.3:77815982-77816001	MS.gene049438:CDS	75.0%
!	CGCGTGGGAGAGGCTGCGCG+TGG	-	chr3.3:77815970-77815989	MS.gene049438:CDS	80.0%
!	GGAGAGGCTGCGCGTGGCGG+AGG	-	chr3.3:77815976-77815995	MS.gene049438:CDS	80.0%
!!	GCGCGTGGCGGAGGAAGCGG+AGG	-	chr3.3:77815985-77816004	MS.gene049438:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	77815818	77816150	77815818	ID=MS.gene049438
chr3.3	mRNA	77815818	77816150	77815818	ID=MS.gene049438.t1;Parent=MS.gene049438
chr3.3	exon	77815818	77816150	77815818	ID=MS.gene049438.t1.exon1;Parent=MS.gene049438.t1
chr3.3	CDS	77815818	77816150	77815818	ID=cds.MS.gene049438.t1;Parent=MS.gene049438.t1

Gene Sequence	>MS.gene049438 ATGATGGCAGTGATCGGCACCGGAGGAAAAAAAGAGTCTAAAAATGCGGTGCCCGTGCCGGAGTCGGAGCTGAAGAGAAGAAACGATCAGCTTGAGAAAGAGTTGAAGGAGAGCAAGGAGAGAGAAGAGCAAATGAGACGGCAGTTACAGAGCGCGTGGGAGAGGCTGCGCGTGGCGGAGGAAGCGGAGGAGAGATTATGCTCACAGCTCGGAGAGCTGGAAGCAGAAGCTGTTTATCAAGCGCGTGATTATCACGATCGTATTGTTTCTCTGATGGATCAGCTCTCACGCGCGCAGAGTCTTCTTCATAATGCTTCTTCCAACTCGTTGTGA
Protein sequence	>MS.gene049438.t1 MMAVIGTGGKKESKNAVPVPESELKRRNDQLEKELKESKEREEQMRRQLQSAWERLRVAEEAEERLCSQLGELEAEAVYQARDYHDRIVSLMDQLSRAQSLLHNASSNSL