AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene013617

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013617.t1 MTR_3g091040 97.724 703 15 1 2 704 1 702 0.0 1441
MS.gene013617.t1 MTR_6g059870 45.306 735 319 14 2 699 1 689 0.0 600
MS.gene013617.t1 MTR_3g099170 40.863 788 353 19 2 697 15 781 0.0 540
MS.gene013617.t1 MTR_3g082810 39.024 738 351 17 2 698 1 680 7.15e-166 493
MS.gene013617.t1 MTR_1g081970 31.549 710 406 20 2 692 1 649 1.82e-104 333
MS.gene013617.t1 MTR_7g114150 31.941 742 423 20 2 704 1 699 1.26e-103 341
MS.gene013617.t1 MTR_7g114150 28.378 222 141 6 472 687 870 1079 4.00e-18 89.7
MS.gene013617.t1 MTR_4g118660 32.089 723 406 17 1 698 41 703 1.61e-102 336
MS.gene013617.t1 MTR_4g118660 36.864 236 125 10 467 691 771 993 1.72e-30 129
MS.gene013617.t1 MTR_2g020420 32.539 713 404 20 2 693 1 657 5.12e-101 331
MS.gene013617.t1 MTR_2g020420 31.646 237 138 8 464 687 701 926 2.51e-23 106
MS.gene013617.t1 MTR_2g020420 32.148 731 396 22 2 693 1 670 1.80e-100 330
MS.gene013617.t1 MTR_2g020420 31.646 237 138 8 464 687 714 939 2.21e-23 106
MS.gene013617.t1 MTR_5g097170 47.619 315 149 6 382 694 384 684 2.77e-90 296
MS.gene013617.t1 MTR_5g097170 43.750 240 99 4 2 239 1 206 6.00e-54 197
MS.gene013617.t1 MTR_1g060440 36.996 273 160 7 429 692 376 645 3.62e-51 189
MS.gene013617.t1 MTR_1g060440 41.964 224 105 7 2 223 1 201 1.23e-39 155
MS.gene013617.t1 MTR_5g029070 41.631 233 129 3 464 694 322 549 7.61e-46 172
MS.gene013617.t1 MTR_5g029070 41.631 233 129 3 464 694 347 574 1.07e-45 172
MS.gene013617.t1 MTR_1g051765 37.339 233 135 6 463 692 140 364 6.85e-44 162
MS.gene013617.t1 MTR_1g081960 36.905 252 117 7 1 239 50 272 1.01e-39 149
MS.gene013617.t1 MTR_8g011080 39.462 223 98 6 1 223 53 238 2.79e-36 146
MS.gene013617.t1 MTR_8g011080 34.286 210 125 4 489 698 419 615 7.56e-32 132
MS.gene013617.t1 MTR_5g066100 33.891 239 139 6 1 229 1 230 6.30e-31 124
MS.gene013617.t1 MTR_3g100330 31.624 234 109 6 2 229 1 189 4.73e-25 104
MS.gene013617.t1 MTR_4g093930 29.502 261 154 7 1 231 1 261 1.14e-20 96.3
MS.gene013617.t1 MTR_2g435450 59.091 66 27 0 66 131 38 103 3.07e-16 80.1
MS.gene013617.t1 MTR_2g009520 32.824 131 87 1 1 130 1 131 1.19e-14 77.8
MS.gene013617.t1 MTR_2g436560 33.735 166 72 2 2 129 1 166 1.95e-14 75.9
MS.gene013617.t1 MTR_1g082220 56.452 62 27 0 2 63 1 62 2.82e-14 72.4
MS.gene013617.t1 MTR_2g436580 32.370 173 71 3 2 129 1 172 1.77e-13 70.9
MS.gene013617.t1 MTR_4g120450 54.545 66 30 0 66 131 36 101 2.50e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013617.t1 AT5G53150 46.505 744 330 18 2 704 1 717 0.0 628
MS.gene013617.t1 AT5G53150 46.505 744 330 18 2 704 1 717 0.0 628
MS.gene013617.t1 AT5G53150 46.505 744 330 18 2 704 1 717 0.0 628
MS.gene013617.t1 AT5G53150 45.833 744 306 18 2 704 1 688 0.0 616
MS.gene013617.t1 AT5G53150 45.833 744 306 18 2 704 1 688 0.0 616
MS.gene013617.t1 AT2G25560 39.326 712 349 17 2 692 1 650 4.27e-146 441
MS.gene013617.t1 AT2G05230 34.635 768 369 21 2 698 1 706 6.59e-136 416
MS.gene013617.t1 AT2G05250 34.635 768 369 21 2 698 1 706 6.59e-136 416
MS.gene013617.t1 AT5G53150 41.971 548 251 17 2 509 1 521 7.95e-122 375
MS.gene013617.t1 AT5G35753 37.949 390 203 10 328 692 48 423 2.54e-76 252
MS.gene013617.t1 AT5G37380 38.955 421 183 10 2 360 1 409 2.13e-75 249
MS.gene013617.t1 AT5G37380 38.955 421 183 10 2 360 1 409 2.13e-75 249
MS.gene013617.t1 AT5G37380 38.955 421 183 10 2 360 1 409 2.13e-75 249
MS.gene013617.t1 AT5G37380 38.955 421 183 10 2 360 1 409 2.13e-75 249
MS.gene013617.t1 AT5G37380 38.955 421 183 10 2 360 1 409 2.13e-75 249
MS.gene013617.t1 AT5G37380 38.955 421 183 10 2 360 1 409 2.13e-75 249
MS.gene013617.t1 AT5G37380 38.955 421 183 10 2 360 1 409 2.13e-75 249
MS.gene013617.t1 AT5G37380 38.955 421 183 10 2 360 1 409 2.13e-75 249
MS.gene013617.t1 AT5G37380 38.955 421 183 10 2 360 1 409 2.13e-75 249
MS.gene013617.t1 AT5G50115 41.935 279 127 4 425 702 227 471 1.06e-65 225
MS.gene013617.t1 AT5G50115 31.013 158 102 2 3 153 10 167 3.66e-15 79.3
MS.gene013617.t1 AT4G19570 44.534 247 111 6 2 223 1 246 1.19e-55 199
MS.gene013617.t1 AT5G37440 42.683 246 96 7 2 245 16 218 9.83e-53 184
MS.gene013617.t1 AT5G37750 42.797 236 89 8 12 245 23 214 1.21e-51 179
MS.gene013617.t1 AT4G19590 63.158 133 48 1 2 133 1 133 5.68e-49 176
MS.gene013617.t1 AT4G19590 63.158 133 48 1 2 133 1 133 7.46e-49 176
MS.gene013617.t1 AT4G19590 32.836 134 74 4 178 295 210 343 2.75e-11 66.2
MS.gene013617.t1 AT3G04980 44.444 225 99 3 2 225 1 200 3.38e-48 184
MS.gene013617.t1 AT3G04980 33.906 233 140 7 465 693 490 712 6.53e-34 140
MS.gene013617.t1 AT3G04980 29.183 257 153 10 446 693 946 1182 3.38e-17 87.0
MS.gene013617.t1 AT3G04980 44.444 225 99 3 2 225 1 200 3.38e-48 184
MS.gene013617.t1 AT3G04980 33.906 233 140 7 465 693 490 712 6.53e-34 140
MS.gene013617.t1 AT3G04980 29.183 257 153 10 446 693 946 1182 3.38e-17 87.0
MS.gene013617.t1 AT4G19580 41.350 237 100 7 2 200 1 236 1.03e-43 160
MS.gene013617.t1 AT4G19590 61.983 121 45 1 14 133 2 122 8.94e-42 155
MS.gene013617.t1 AT3G04960 39.245 265 135 10 434 687 235 484 1.38e-41 159
MS.gene013617.t1 AT3G04960 39.245 265 135 10 434 687 284 533 2.35e-41 159
MS.gene013617.t1 AT5G18750 37.607 234 139 4 465 692 396 628 2.86e-41 162
MS.gene013617.t1 AT5G18750 33.793 290 171 6 5 281 3 284 1.82e-34 141
MS.gene013617.t1 AT5G18750 34.052 232 142 7 464 690 656 881 7.96e-30 126
MS.gene013617.t1 AT3G04960 39.245 265 135 10 434 687 287 536 4.15e-41 158
MS.gene013617.t1 AT3G04960 39.245 265 135 10 434 687 336 585 6.78e-41 159
MS.gene013617.t1 AT5G27240 38.197 233 131 6 463 693 471 692 2.62e-40 159
MS.gene013617.t1 AT5G27240 38.393 224 108 2 2 225 1 194 7.89e-40 158
MS.gene013617.t1 AT5G27240 29.478 268 158 11 406 662 825 1072 2.04e-18 90.9
MS.gene013617.t1 AT5G27240 38.197 233 131 6 463 693 471 692 2.62e-40 159
MS.gene013617.t1 AT5G27240 38.393 224 108 2 2 225 1 194 7.89e-40 158
MS.gene013617.t1 AT5G27240 29.478 268 158 11 406 662 825 1072 2.04e-18 90.9
MS.gene013617.t1 AT5G27240 38.197 233 131 6 463 693 471 692 2.62e-40 159
MS.gene013617.t1 AT5G27240 38.393 224 108 2 2 225 1 194 7.89e-40 158
MS.gene013617.t1 AT5G27240 29.478 268 158 11 406 662 825 1072 2.04e-18 90.9
MS.gene013617.t1 AT3G04980 43.350 203 89 3 24 225 5 182 3.67e-40 159
MS.gene013617.t1 AT3G04980 33.906 233 140 7 465 693 472 694 6.57e-34 140
MS.gene013617.t1 AT3G04980 29.183 257 153 10 446 693 928 1164 3.69e-17 86.7
MS.gene013617.t1 AT3G06340 38.095 231 133 5 2 225 1 228 2.00e-38 152
MS.gene013617.t1 AT3G06340 36.957 230 137 4 465 692 428 651 3.01e-35 142
MS.gene013617.t1 AT3G06340 38.095 231 133 5 2 225 1 228 2.00e-38 152
MS.gene013617.t1 AT3G06340 36.957 230 137 4 465 692 428 651 3.01e-35 142
MS.gene013617.t1 AT3G06340 38.095 231 133 5 2 225 1 228 2.00e-38 152
MS.gene013617.t1 AT3G06340 36.957 230 137 4 465 692 428 651 3.01e-35 142
MS.gene013617.t1 AT3G06340 38.095 231 133 5 2 225 1 228 2.00e-38 152
MS.gene013617.t1 AT3G06340 36.957 230 137 4 465 692 428 651 3.01e-35 142
MS.gene013617.t1 AT5G18740 32.365 241 147 4 472 698 174 412 3.05e-33 133
MS.gene013617.t1 AT4G27980 33.333 240 144 9 453 687 301 529 4.72e-30 125
MS.gene013617.t1 AT4G27980 33.186 226 138 7 464 687 353 567 7.39e-30 125
MS.gene013617.t1 AT4G27980 33.186 226 138 7 464 687 352 566 7.48e-30 125
MS.gene013617.t1 AT2G01710 32.189 233 118 5 5 229 7 207 8.07e-29 117
MS.gene013617.t1 AT2G01710 32.189 233 118 5 5 229 51 251 1.44e-28 118
MS.gene013617.t1 AT2G35540 33.480 227 140 6 469 691 368 587 6.70e-28 119
MS.gene013617.t1 AT2G35540 30.374 214 111 2 8 221 12 187 1.61e-25 112
MS.gene013617.t1 AT5G18730 31.818 242 148 6 472 698 44 283 1.24e-27 118
MS.gene013617.t1 AT5G18730 27.586 232 137 9 484 692 304 527 1.04e-12 71.6
MS.gene013617.t1 AT3G05110 33.488 215 126 6 464 675 158 358 1.97e-27 115
MS.gene013617.t1 AT5G64360 30.594 219 140 6 16 230 25 235 1.28e-21 98.6
MS.gene013617.t1 AT5G37760 41.606 137 50 2 14 150 79 185 1.30e-21 94.0
MS.gene013617.t1 AT5G64360 30.594 219 140 6 16 230 25 235 1.73e-21 98.6
MS.gene013617.t1 AT5G64360 30.594 219 140 6 16 230 25 235 1.73e-21 98.6
MS.gene013617.t1 AT5G64360 30.594 219 140 6 16 230 25 235 1.73e-21 98.6
MS.gene013617.t1 AT5G64360 30.594 219 140 6 16 230 25 235 1.73e-21 98.6
MS.gene013617.t1 AT5G64360 30.594 219 140 6 16 230 25 235 1.73e-21 98.6
MS.gene013617.t1 AT5G64360 30.594 219 140 6 16 230 25 235 1.73e-21 98.6
MS.gene013617.t1 AT5G62780 68.519 54 17 0 52 105 3 56 1.60e-18 85.1
MS.gene013617.t1 AT5G09540 28.879 232 133 6 3 230 8 211 1.80e-18 86.7
MS.gene013617.t1 AT5G50510 34.711 121 74 1 3 123 10 125 3.93e-15 75.9
MS.gene013617.t1 AT5G50620 34.711 121 74 1 3 123 10 125 3.93e-15 75.9
MS.gene013617.t1 AT3G06778 46.154 78 42 0 54 131 29 106 2.81e-14 73.2
MS.gene013617.t1 AT1G09260 35.870 92 55 2 44 134 50 138 8.54e-14 69.3
MS.gene013617.t1 AT5G18720 27.426 237 131 9 484 691 114 338 1.29e-13 73.2
MS.gene013617.t1 AT5G18720 27.426 237 131 9 484 691 114 338 1.29e-13 73.2
MS.gene013617.t1 AT5G18720 27.426 237 131 9 484 691 114 338 1.29e-13 73.2
MS.gene013617.t1 AT5G49060 31.579 171 84 3 2 139 1 171 1.32e-12 70.5
MS.gene013617.t1 AT5G49060 31.579 171 84 3 2 139 1 171 1.32e-12 70.5
MS.gene013617.t1 AT1G62970 29.851 134 81 4 16 136 18 151 6.89e-12 69.3
MS.gene013617.t1 AT3G57340 43.836 73 41 0 56 128 102 174 4.16e-11 65.9
MS.gene013617.t1 AT3G57340 43.836 73 41 0 56 128 102 174 4.16e-11 65.9
MS.gene013617.t1 AT5G05750 44.118 68 38 0 61 128 108 175 8.03e-11 64.3

Find 171 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAAACTTGTGGGGATTTC+AGG 0.135241 3.4:-83440457 MS.gene013617:CDS
ACTCCCCTTGTTAAGTTTCC+TGG 0.260877 3.4:-83440206 MS.gene013617:CDS
GCAGATGATATTCGCGTTAA+TGG 0.268418 3.4:-83441115 MS.gene013617:CDS
TTCTGCCCCTCCACAGAAAA+AGG 0.278721 3.4:-83441542 MS.gene013617:CDS
TTGTTAAGTTTCCTGGCTTT+AGG 0.285789 3.4:-83440199 MS.gene013617:CDS
GCAGGTTTGGGCAGTTTCTC+TGG 0.291233 3.4:-83441046 MS.gene013617:CDS
CAACTAATCCGCATTCTAAA+TGG 0.298217 3.4:+83440539 None:intergenic
CAGGATGACGTCGTGAATTC+TGG 0.305004 3.4:+83441347 None:intergenic
CCAATAGATTGGGTAGGTTC+TGG 0.312215 3.4:-83440485 MS.gene013617:CDS
CACTCCAGAATGGCTGGTTT+TGG 0.313195 3.4:-83441292 MS.gene013617:CDS
CCAGAACCTACCCAATCTAT+TGG 0.320518 3.4:+83440485 None:intergenic
TCCAACTCTCGGCAACCTAT+TGG 0.323407 3.4:+83440056 None:intergenic
CTGGTTATATTCTAGTCTCT+TGG 0.331341 3.4:+83441714 None:intergenic
CTCTCGAAGCAACAGTGAAT+TGG 0.332588 3.4:-83440510 MS.gene013617:CDS
AATCGAAACATCTCCTCTTT+AGG 0.335882 3.4:+83440131 None:intergenic
CCTTTGCAGAGGACCAGGTC+TGG 0.336859 3.4:-83440631 MS.gene013617:CDS
GCTACCAAAACCAGCCATTC+TGG 0.342521 3.4:+83441288 None:intergenic
TTTGAACTAGGTACATTTGA+TGG 0.348433 3.4:+83441640 None:intergenic
GGAGTTGTCCGCATCTTTCC+AGG 0.349771 3.4:-83440362 MS.gene013617:CDS
ACTTGAAGAATGGAGGTTAA+TGG 0.359594 3.4:-83440882 MS.gene013617:CDS
AGCCTTTCAAACTCCTCTTC+TGG 0.362339 3.4:+83441695 None:intergenic
GGATCTGGAGCAGCAGGTTT+GGG 0.373731 3.4:-83441058 MS.gene013617:CDS
CATTTAGAATGCGGATTAGT+TGG 0.373866 3.4:-83440538 MS.gene013617:CDS
GGGATCTGGAGCAGCAGGTT+TGG 0.384095 3.4:-83441059 MS.gene013617:CDS
GAATCCTCCTAACCTCATTC+TGG 0.385216 3.4:+83440153 None:intergenic
TTGGAGCGTTATGAGCTTCC+TGG 0.385283 3.4:+83440075 None:intergenic
AGGTTTGAATTATTACTTCC+AGG 0.385391 3.4:+83441328 None:intergenic
TGATGGGAGGGCAAACTTGA+TGG 0.385605 3.4:+83441376 None:intergenic
ATAGAAACTGGTCTCCTGAT+TGG 0.387089 3.4:-83440316 MS.gene013617:CDS
CTTTGCAGAGGACCAGGTCT+GGG 0.387294 3.4:-83440630 MS.gene013617:CDS
GTGAATTCTGGTGCTGTTGA+TGG 0.390462 3.4:+83441359 None:intergenic
TATGGAGAGTCCAATGTTAA+TGG 0.390480 3.4:-83440785 MS.gene013617:CDS
CTCTCCCATTCAGCAATTGA+TGG 0.393875 3.4:+83441184 None:intergenic
AGGTCAAAATTGTGAAAATC+AGG 0.395280 3.4:+83440671 None:intergenic
GAACAAAGTAAGTGGAGAAA+TGG 0.401074 3.4:-83441902 MS.gene013617:CDS
GCAACTTCAAATGTGAGGTC+TGG 0.403438 3.4:-83441592 MS.gene013617:CDS
TTGGGCAGTTTCTCTGGATC+AGG 0.403555 3.4:-83441040 MS.gene013617:CDS
GTGAATTGGGCCCAATAGAT+TGG 0.406953 3.4:-83440496 MS.gene013617:CDS
TCTGGGCACTTTATAGAAAC+TGG 0.408209 3.4:-83440328 MS.gene013617:CDS
ACAACTGGTTCAGAGAAGTA+TGG 0.411674 3.4:-83440803 MS.gene013617:CDS
GATGTTTCGATTCTCTCATC+AGG 0.412444 3.4:-83440120 MS.gene013617:CDS
TCCTAACCTCATTCTGGTCC+TGG 0.413595 3.4:+83440159 None:intergenic
TGCAAAGAAGTTTGCTATCA+AGG 0.415023 3.4:-83441998 MS.gene013617:CDS
AGCACCATCAATTGCTGAAT+GGG 0.416490 3.4:-83441188 MS.gene013617:CDS
CTATGTTACAATCAATGTTC+CGG 0.418874 3.4:-83440696 MS.gene013617:CDS
GCAACAAAGATGAGGCAGTC+AGG 0.422134 3.4:-83442068 MS.gene013617:CDS
TAATGGCTATCCAGGATATA+CGG 0.422893 3.4:-83441098 MS.gene013617:CDS
TGGATCAGGAAAGGCTAACT+TGG 0.429304 3.4:-83441026 MS.gene013617:CDS
TGTCCGCATCTTTCCAGGTA+AGG 0.431625 3.4:-83440357 MS.gene013617:CDS
TACTTGTGTATCACTTCATC+TGG 0.431902 3.4:+83440281 None:intergenic
GGGAGGAGGACTCCTCTCTA+TGG 0.433642 3.4:+83441411 None:intergenic
GATGGATGCCCAGCACGGTT+TGG 0.435679 3.4:+83441658 None:intergenic
CCGCATTCTAAATGGCTTTG+TGG 0.437694 3.4:+83440547 None:intergenic
CGGAACATTGATTGTAACAT+AGG 0.437900 3.4:+83440697 None:intergenic
TCTCGAAGCAACAGTGAATT+GGG 0.438567 3.4:-83440509 MS.gene013617:CDS
TGCAGAGGGTGCATTTCAGC+TGG 0.443695 3.4:-83441770 MS.gene013617:CDS
TAGAAAGCAGTACAGGAAAC+TGG 0.447905 3.4:-83441830 MS.gene013617:CDS
TGAATTCTAGCATAATATCT+AGG 0.449645 3.4:+83440581 None:intergenic
TTCAGCTGGTTTCAGAGGCA+TGG 0.452089 3.4:-83441756 MS.gene013617:CDS
ATTCGCGTTAATGGCTATCC+AGG 0.452896 3.4:-83441106 MS.gene013617:CDS
CACAAAAGCTTCATTACAGT+TGG 0.453452 3.4:+83441435 None:intergenic
CTTATTACAGATAGTCCAAA+AGG 0.455028 3.4:+83441513 None:intergenic
TGGCCACTGAGCAAGTGATT+AGG 0.456167 3.4:+83440095 None:intergenic
CAGAACCTACCCAATCTATT+GGG 0.457508 3.4:+83440486 None:intergenic
CAACTTCAAATGTGAGGTCT+GGG 0.460913 3.4:-83441591 MS.gene013617:CDS
TCAAATGTACCTAGTTCAAA+TGG 0.462096 3.4:-83441637 MS.gene013617:CDS
CTGATGGTTCTATGAAGAAA+AGG 0.462638 3.4:-83441141 MS.gene013617:CDS
TGAGTTACGAAACATGTTGA+TGG 0.462926 3.4:-83440933 MS.gene013617:CDS
AAAGATGCGGACAACTCCTC+TGG 0.465447 3.4:+83440367 None:intergenic
ATGGATTGGTATGGTATACT+TGG 0.466628 3.4:-83441883 MS.gene013617:CDS
TAATCACTTGCTCAGTGGCC+AGG 0.470311 3.4:-83440093 MS.gene013617:CDS
GGTTCCCACTCCAGAATGGC+TGG 0.472268 3.4:-83441298 MS.gene013617:CDS
AAATTTGTATGCTGATTTGG+AGG 0.472377 3.4:-83441971 MS.gene013617:CDS
GAAGCTCATAACGCTCCAAT+AGG 0.472605 3.4:-83440071 MS.gene013617:CDS
ATGCTTGTTACCATTAACAT+TGG 0.473523 3.4:+83440775 None:intergenic
CACAAGGTTAGGTGGACAAA+AGG 0.475228 3.4:-83440392 MS.gene013617:CDS
ATATTCAATGACAAAACAAC+TGG 0.477357 3.4:-83440818 MS.gene013617:CDS
CATACTACTCCAAACCGTGC+TGG 0.481237 3.4:-83441667 MS.gene013617:CDS
GCTACGGGATCTGGAGCAGC+AGG 0.481387 3.4:-83441064 MS.gene013617:CDS
AAGTTCCTTTGCAGAGGACC+AGG 0.482078 3.4:-83440636 MS.gene013617:CDS
AAATGATTTACCGTATATCC+TGG 0.486418 3.4:+83441088 None:intergenic
GGAGTATGTTCATTCCAATC+AGG 0.487114 3.4:+83440302 None:intergenic
TGAATTGGGCCCAATAGATT+GGG 0.488808 3.4:-83440495 MS.gene013617:CDS
AGGAACTTTCAGCTCTATCC+AGG 0.489052 3.4:+83440651 None:intergenic
AGGTCTAAAGGTGTAGCTGC+TGG 0.492454 3.4:+83440032 None:intergenic
ACGAAACATGTTGATGGATA+AGG 0.495669 3.4:-83440927 MS.gene013617:CDS
CAGAATTCACGACGTCATCC+TGG 0.496907 3.4:-83441346 MS.gene013617:CDS
CACGGTTTGGAGTAGTATGT+TGG 0.497106 3.4:+83441671 None:intergenic
GTCCGCATCTTTCCAGGTAA+GGG 0.498134 3.4:-83440356 MS.gene013617:CDS
GATAGCAACAGAAGCTAATG+AGG 0.502266 3.4:-83440003 MS.gene013617:CDS
GTCTGGGAATCATAATGCTC+GGG 0.503789 3.4:-83441575 MS.gene013617:CDS
GGACTATCTGTAATAAGTGC+AGG 0.505271 3.4:-83441507 MS.gene013617:CDS
GACAGTATTTCGAAGGCACC+AGG 0.509786 3.4:-83440177 MS.gene013617:CDS
CAAAACCAGCCATTCTGGAG+TGG 0.511268 3.4:+83441293 None:intergenic
TCCAATAGGTTGCCGAGAGT+TGG 0.511728 3.4:-83440057 MS.gene013617:CDS
TGTGGAGGGGCAGAAGTGGC+AGG 0.513069 3.4:+83441550 None:intergenic
CATTGATTGTAACATAGGCT+TGG 0.515798 3.4:+83440702 None:intergenic
CGAAATACTGTCCTAAAGCC+AGG 0.516363 3.4:+83440188 None:intergenic
ATACTACTCCAAACCGTGCT+GGG 0.517498 3.4:-83441666 MS.gene013617:CDS
GAGCACCATCAATTGCTGAA+TGG 0.520958 3.4:-83441189 MS.gene013617:CDS
AACAGTCTCTTCATCAGCAA+AGG 0.522182 3.4:+83441852 None:intergenic
AGACTGTTAGAAAGCAGTAC+AGG 0.523671 3.4:-83441837 MS.gene013617:CDS
TGCATTTCAGCTGGTTTCAG+AGG 0.526061 3.4:-83441761 MS.gene013617:CDS
GCTGCTTATGACGATGATGA+TGG 0.528010 3.4:-83440608 MS.gene013617:CDS
TCTGCTGAGAACAAAGTAAG+TGG 0.530077 3.4:-83441910 MS.gene013617:CDS
CGTCATAAGCAGCCCAGACC+TGG 0.530148 3.4:+83440618 None:intergenic
TCTTTACCTGTCACATCTGA+TGG 0.530274 3.4:-83440740 MS.gene013617:CDS
TAGCTTCTGTTGCTATCTGA+AGG 0.531060 3.4:+83440009 None:intergenic
TGAATTCTGGTGCTGTTGAT+GGG 0.531536 3.4:+83441360 None:intergenic
AAATTGTGAAAATCAGGATC+CGG 0.532427 3.4:+83440677 None:intergenic
TAATAATTCAAACCTTCAGT+GGG 0.534646 3.4:-83441320 MS.gene013617:CDS
GAGGATTCAAATGTCTAAGA+AGG 0.536968 3.4:-83441164 MS.gene013617:CDS
AGCTGCAAGTGTCGTGCAGA+AGG 0.538362 3.4:-83441236 MS.gene013617:CDS
ACAAAAGCTTCATTACAGTT+GGG 0.539482 3.4:+83441436 None:intergenic
ACCAGGACCAGAATGAGGTT+AGG 0.540017 3.4:-83440160 MS.gene013617:CDS
ACTTGTGGGGATTTCAGGAC+TGG 0.540134 3.4:-83440452 MS.gene013617:CDS
ACAGAAGCTAATGAGGCAGC+AGG 0.543005 3.4:-83439996 MS.gene013617:CDS
CATCAATTGCTGAATGGGAG+AGG 0.543526 3.4:-83441183 MS.gene013617:CDS
GCTATCTGAAGGAGGTCTAA+AGG 0.548645 3.4:+83440020 None:intergenic
CACGACACTTGCAGCTTGAG+CGG 0.548940 3.4:+83441243 None:intergenic
ACAGTCTCTTCATCAGCAAA+GGG 0.551588 3.4:+83441853 None:intergenic
AAGTAAGTGGAGAAATGGAT+TGG 0.558705 3.4:-83441897 MS.gene013617:CDS
CCAGACCTGGTCCTCTGCAA+AGG 0.559516 3.4:+83440631 None:intergenic
TTTCTGTGGAGGGGCAGAAG+TGG 0.559602 3.4:+83441546 None:intergenic
TGATAATAACCATTTGAACT+AGG 0.560472 3.4:+83441628 None:intergenic
GGTTAGGAGGATTCCTAAAG+AGG 0.561219 3.4:-83440144 MS.gene013617:CDS
CAACAAAGATGAGGCAGTCA+GGG 0.562873 3.4:-83442067 MS.gene013617:CDS
GTGGGGTTCCCACTCCAGAA+TGG 0.563354 3.4:-83441302 MS.gene013617:CDS
AGTGGAGAAATGGATTGGTA+TGG 0.565199 3.4:-83441892 MS.gene013617:CDS
TCCGCATCTTTCCAGGTAAG+GGG 0.566183 3.4:-83440355 MS.gene013617:CDS
GACTAGAATATAACCAGAAG+AGG 0.567369 3.4:-83441708 MS.gene013617:CDS
GGTCTGGGAATCATAATGCT+CGG 0.568311 3.4:-83441576 MS.gene013617:CDS
AACCAGAAGAGGAGTTTGAA+AGG 0.568957 3.4:-83441697 MS.gene013617:CDS
AACAAGTCTTTAGGTGCAGA+GGG 0.573429 3.4:-83441784 MS.gene013617:CDS
AGCTGAAAGTTCCTTTGCAG+AGG 0.574398 3.4:-83440642 MS.gene013617:CDS
CAAATGTCTAAGAAGGCTGA+TGG 0.579017 3.4:-83441157 MS.gene013617:CDS
GCTTTAGGACAGTATTTCGA+AGG 0.582482 3.4:-83440184 MS.gene013617:CDS
ACTGTACCATCAGATGTGAC+AGG 0.585213 3.4:+83440734 None:intergenic
GGCAACAGACGACGATCCAT+CGG 0.586319 3.4:+83441264 None:intergenic
TGGAGTGGGAACCCCACTGA+AGG 0.587012 3.4:+83441308 None:intergenic
CTTCTGTTGCTATCTGAAGG+AGG 0.589865 3.4:+83440012 None:intergenic
AAACAAGTCTTTAGGTGCAG+AGG 0.591608 3.4:-83441785 MS.gene013617:CDS
ATTCTGGTGCTGTTGATGGG+AGG 0.596016 3.4:+83441363 None:intergenic
CAGTTTCTCTGGATCAGGAA+AGG 0.599343 3.4:-83441035 MS.gene013617:CDS
AGGACCAGAATGAGGTTAGG+AGG 0.601398 3.4:-83440157 MS.gene013617:CDS
TTCTGGTGCTGTTGATGGGA+GGG 0.601651 3.4:+83441364 None:intergenic
AGTGAGAAAGTGAAGAGAGA+AGG 0.603158 3.4:-83441211 MS.gene013617:CDS
GTAATAATTCAAACCTTCAG+TGG 0.605977 3.4:-83441321 MS.gene013617:CDS
AAAACCAGCCATTCTGGAGT+GGG 0.608477 3.4:+83441294 None:intergenic
CCACAAAGCCATTTAGAATG+CGG 0.614075 3.4:-83440547 MS.gene013617:CDS
CCGCATCTTTCCAGGTAAGG+GGG 0.621724 3.4:-83440354 MS.gene013617:CDS
AAGGCACCAGGACCAGAATG+AGG 0.626567 3.4:-83440165 MS.gene013617:CDS
GATGGAGTGCAACAAAGATG+AGG 0.628263 3.4:-83442076 MS.gene013617:CDS
TAAAGCCAGGAAACTTAACA+AGG 0.631614 3.4:+83440201 None:intergenic
TTGGGCCCAATAGATTGGGT+AGG 0.631748 3.4:-83440491 MS.gene013617:CDS
TAGCTGCTGGATCCAACTCT+CGG 0.634962 3.4:+83440045 None:intergenic
ACATGTTGATGGATAAGGCA+CGG 0.636563 3.4:-83440922 MS.gene013617:CDS
TGGTTCTATGAAGAAAAGGA+AGG 0.636867 3.4:-83441137 MS.gene013617:CDS
AGTCAGGGCTAAAGAAATAG+CGG 0.640488 3.4:-83442052 MS.gene013617:CDS
CATTTGATGGATGCCCAGCA+CGG 0.640859 3.4:+83441653 None:intergenic
AGAATGCAACTTCAAATGTG+AGG 0.642079 3.4:-83441597 MS.gene013617:CDS
GATGATTTCAGTGAAGAGCA+AGG 0.643056 3.4:-83440239 MS.gene013617:CDS
AAGCCAGGAAACTTAACAAG+GGG 0.655417 3.4:+83440203 None:intergenic
AGGTGGACAAAAGGCACCAG+AGG 0.656547 3.4:-83440383 MS.gene013617:CDS
AAAGCCAGGAAACTTAACAA+GGG 0.677929 3.4:+83440202 None:intergenic
GTTCCTAATCACTTGCTCAG+TGG 0.678955 3.4:-83440098 MS.gene013617:CDS
ATATTATGCTAGAATTCACA+AGG 0.689430 3.4:-83440576 MS.gene013617:CDS
AATAATTCAAACCTTCAGTG+GGG 0.716457 3.4:-83441319 MS.gene013617:CDS
GATACACAAGTATGACATGG+TGG 0.719131 3.4:-83440270 MS.gene013617:CDS
GGGCTAAAGAAATAGCGGAG+AGG 0.758438 3.4:-83442047 MS.gene013617:CDS
AGTGATACACAAGTATGACA+TGG 0.781290 3.4:-83440273 MS.gene013617:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGTAAAAAACTTGAAGAA+TGG - chr3.4:83441172-83441191 MS.gene013617:CDS 20.0%
!!! ATTAAATTCATTTTCACACA+AGG - chr3.4:83441656-83441675 MS.gene013617:CDS 20.0%
!!! TAAAAATTTGTATGCTGATT+TGG - chr3.4:83440090-83440109 MS.gene013617:CDS 20.0%
! ATATTATGCTAGAATTCACA+AGG - chr3.4:83441488-83441507 MS.gene013617:CDS 25.0%
! ATATTCAATGACAAAACAAC+TGG - chr3.4:83441246-83441265 MS.gene013617:CDS 25.0%
! ATCAATAGTAGTCAAAAACT+GGG + chr3.4:83440125-83440144 None:intergenic 25.0%
! TAATAATTCAAACCTTCAGT+GGG - chr3.4:83440744-83440763 MS.gene013617:CDS 25.0%
! TATCAATAGTAGTCAAAAAC+TGG + chr3.4:83440126-83440145 None:intergenic 25.0%
! TGAATTCTAGCATAATATCT+AGG + chr3.4:83441486-83441505 None:intergenic 25.0%
! TGATAATAACCATTTGAACT+AGG + chr3.4:83440439-83440458 None:intergenic 25.0%
!! TGGTTTTTATAAAACTTGTG+GGG - chr3.4:83441599-83441618 MS.gene013617:CDS 25.0%
!!! CTGGTTTTTATAAAACTTGT+GGG - chr3.4:83441598-83441617 MS.gene013617:CDS 25.0%
!!! TCTGGTTTTTATAAAACTTG+TGG - chr3.4:83441597-83441616 MS.gene013617:CDS 25.0%
!!! TTTTCTGAAAGGGAATATAT+TGG - chr3.4:83440043-83440062 MS.gene013617:CDS 25.0%
AAATGATTTACCGTATATCC+TGG + chr3.4:83440979-83440998 None:intergenic 30.0%
AAATTGTGAAAATCAGGATC+CGG + chr3.4:83441390-83441409 None:intergenic 30.0%
AATAATTCAAACCTTCAGTG+GGG - chr3.4:83440745-83440764 MS.gene013617:CDS 30.0%
ACAAAAGCTTCATTACAGTT+GGG + chr3.4:83440631-83440650 None:intergenic 30.0%
AGGTCAAAATTGTGAAAATC+AGG + chr3.4:83441396-83441415 None:intergenic 30.0%
AGGTTTGAATTATTACTTCC+AGG + chr3.4:83440739-83440758 None:intergenic 30.0%
CTATGTTACAATCAATGTTC+CGG - chr3.4:83441368-83441387 MS.gene013617:CDS 30.0%
CTTATTACAGATAGTCCAAA+AGG + chr3.4:83440554-83440573 None:intergenic 30.0%
GTAAAAAACTTGAAGAATGG+AGG - chr3.4:83441175-83441194 MS.gene013617:CDS 30.0%
GTAATAATTCAAACCTTCAG+TGG - chr3.4:83440743-83440762 MS.gene013617:CDS 30.0%
TCAAATGTACCTAGTTCAAA+TGG - chr3.4:83440427-83440446 MS.gene013617:CDS 30.0%
TTTGAACTAGGTACATTTGA+TGG + chr3.4:83440427-83440446 None:intergenic 30.0%
! ACTTGATGGCTTAAAAACAT+TGG + chr3.4:83440677-83440696 None:intergenic 30.0%
! ATGCTTGTTACCATTAACAT+TGG + chr3.4:83441292-83441311 None:intergenic 30.0%
! ATTCATTTTCACACAAGGTT+AGG - chr3.4:83441661-83441680 MS.gene013617:CDS 30.0%
! CTTGATGGCTTAAAAACATT+GGG + chr3.4:83440676-83440695 None:intergenic 30.0%
! TACGGTAAATCATTTTGCTA+CGG - chr3.4:83440984-83441003 MS.gene013617:CDS 30.0%
!! AAATTTGTATGCTGATTTGG+AGG - chr3.4:83440093-83440112 MS.gene013617:CDS 30.0%
!! TGATTTTCACAATTTTGACC+TGG - chr3.4:83441395-83441414 MS.gene013617:CDS 30.0%
!!! CTAAAGACTTGTTTTTGTCA+GGG + chr3.4:83440273-83440292 None:intergenic 30.0%
AAAGCCAGGAAACTTAACAA+GGG + chr3.4:83441865-83441884 None:intergenic 35.0%
AAGTAAGTGGAGAAATGGAT+TGG - chr3.4:83440167-83440186 MS.gene013617:CDS 35.0%
AATCGAAACATCTCCTCTTT+AGG + chr3.4:83441936-83441955 None:intergenic 35.0%
ACTTGAAGAATGGAGGTTAA+TGG - chr3.4:83441182-83441201 MS.gene013617:CDS 35.0%
AGAATGCAACTTCAAATGTG+AGG - chr3.4:83440467-83440486 MS.gene013617:CDS 35.0%
AGGCTAACTTGGAAAAAGAA+AGG - chr3.4:83441049-83441068 MS.gene013617:CDS 35.0%
AGTGATACACAAGTATGACA+TGG - chr3.4:83441791-83441810 MS.gene013617:CDS 35.0%
ATAAAACTTGTGGGGATTTC+AGG - chr3.4:83441607-83441626 MS.gene013617:CDS 35.0%
CAACTAATCCGCATTCTAAA+TGG + chr3.4:83441528-83441547 None:intergenic 35.0%
CACAAAAGCTTCATTACAGT+TGG + chr3.4:83440632-83440651 None:intergenic 35.0%
CCTGACAAAAACAAGTCTTT+AGG - chr3.4:83440271-83440290 MS.gene013617:CDS 35.0%
CTGGTTATATTCTAGTCTCT+TGG + chr3.4:83440353-83440372 None:intergenic 35.0%
GAACAAAGTAAGTGGAGAAA+TGG - chr3.4:83440162-83440181 MS.gene013617:CDS 35.0%
GACTAGAATATAACCAGAAG+AGG - chr3.4:83440356-83440375 MS.gene013617:CDS 35.0%
GAGGATTCAAATGTCTAAGA+AGG - chr3.4:83440900-83440919 MS.gene013617:CDS 35.0%
TAAAGCCAGGAAACTTAACA+AGG + chr3.4:83441866-83441885 None:intergenic 35.0%
TAATGGCTATCCAGGATATA+CGG - chr3.4:83440966-83440985 MS.gene013617:CDS 35.0%
TACTTGTGTATCACTTCATC+TGG + chr3.4:83441786-83441805 None:intergenic 35.0%
TATGGAGAGTCCAATGTTAA+TGG - chr3.4:83441279-83441298 MS.gene013617:CDS 35.0%
TGAGTTACGAAACATGTTGA+TGG - chr3.4:83441131-83441150 MS.gene013617:CDS 35.0%
TTGGAAAAAGAAAGGACTTG+TGG - chr3.4:83441057-83441076 MS.gene013617:CDS 35.0%
! ACGAAACATGTTGATGGATA+AGG - chr3.4:83441137-83441156 MS.gene013617:CDS 35.0%
! ACGGTAAATCATTTTGCTAC+GGG - chr3.4:83440985-83441004 MS.gene013617:CDS 35.0%
! AGAAAAAGGCTGAAACCTTT+TGG - chr3.4:83440536-83440555 MS.gene013617:CDS 35.0%
! CAACTGTAATGAAGCTTTTG+TGG - chr3.4:83440630-83440649 MS.gene013617:CDS 35.0%
! CATTGATTGTAACATAGGCT+TGG + chr3.4:83441365-83441384 None:intergenic 35.0%
! CGGAACATTGATTGTAACAT+AGG + chr3.4:83441370-83441389 None:intergenic 35.0%
! CTGATGGTTCTATGAAGAAA+AGG - chr3.4:83440923-83440942 MS.gene013617:CDS 35.0%
! TGGTTCTATGAAGAAAAGGA+AGG - chr3.4:83440927-83440946 MS.gene013617:CDS 35.0%
! TTGTTAAGTTTCCTGGCTTT+AGG - chr3.4:83441865-83441884 MS.gene013617:CDS 35.0%
!! ATGGATTGGTATGGTATACT+TGG - chr3.4:83440181-83440200 MS.gene013617:CDS 35.0%
!! CATTTAGAATGCGGATTAGT+TGG - chr3.4:83441526-83441545 MS.gene013617:CDS 35.0%
!! GGAGAGGAAATTTTCTGAAA+GGG - chr3.4:83440033-83440052 MS.gene013617:CDS 35.0%
!! TGCAAAGAAGTTTGCTATCA+AGG - chr3.4:83440066-83440085 MS.gene013617:CDS 35.0%
!!! CCTAAAGACTTGTTTTTGTC+AGG + chr3.4:83440274-83440293 None:intergenic 35.0%
AAACAAGTCTTTAGGTGCAG+AGG - chr3.4:83440279-83440298 MS.gene013617:CDS 40.0%
AACAAGTCTTTAGGTGCAGA+GGG - chr3.4:83440280-83440299 MS.gene013617:CDS 40.0%
AACAGTCTCTTCATCAGCAA+AGG + chr3.4:83440215-83440234 None:intergenic 40.0%
AACCAGAAGAGGAGTTTGAA+AGG - chr3.4:83440367-83440386 MS.gene013617:CDS 40.0%
AAGCCAGGAAACTTAACAAG+GGG + chr3.4:83441864-83441883 None:intergenic 40.0%
ACAGTCTCTTCATCAGCAAA+GGG + chr3.4:83440214-83440233 None:intergenic 40.0%
AGACTGTTAGAAAGCAGTAC+AGG - chr3.4:83440227-83440246 MS.gene013617:CDS 40.0%
AGCACCATCAATTGCTGAAT+GGG - chr3.4:83440876-83440895 MS.gene013617:CDS 40.0%
AGTCAGGGCTAAAGAAATAG+CGG - chr3.4:83440012-83440031 MS.gene013617:CDS 40.0%
AGTGAGAAAGTGAAGAGAGA+AGG - chr3.4:83440853-83440872 MS.gene013617:CDS 40.0%
CAAATGTCTAAGAAGGCTGA+TGG - chr3.4:83440907-83440926 MS.gene013617:CDS 40.0%
CAACTTCAAATGTGAGGTCT+GGG - chr3.4:83440473-83440492 MS.gene013617:CDS 40.0%
CAGAACCTACCCAATCTATT+GGG + chr3.4:83441581-83441600 None:intergenic 40.0%
GATACACAAGTATGACATGG+TGG - chr3.4:83441794-83441813 MS.gene013617:CDS 40.0%
GATAGCAACAGAAGCTAATG+AGG - chr3.4:83442061-83442080 MS.gene013617:CDS 40.0%
GATGATTTCAGTGAAGAGCA+AGG - chr3.4:83441825-83441844 MS.gene013617:CDS 40.0%
GATGGCTTAAAAACATTGGG+AGG + chr3.4:83440673-83440692 None:intergenic 40.0%
GATGTTTCGATTCTCTCATC+AGG - chr3.4:83441944-83441963 MS.gene013617:CDS 40.0%
GCAGATGATATTCGCGTTAA+TGG - chr3.4:83440949-83440968 MS.gene013617:CDS 40.0%
GGACTATCTGTAATAAGTGC+AGG - chr3.4:83440557-83440576 MS.gene013617:CDS 40.0%
GGAGTATGTTCATTCCAATC+AGG + chr3.4:83441765-83441784 None:intergenic 40.0%
TAGAAAGCAGTACAGGAAAC+TGG - chr3.4:83440234-83440253 MS.gene013617:CDS 40.0%
TAGCTTCTGTTGCTATCTGA+AGG + chr3.4:83442058-83442077 None:intergenic 40.0%
TCTCGAAGCAACAGTGAATT+GGG - chr3.4:83441555-83441574 MS.gene013617:CDS 40.0%
TCTGCTGAGAACAAAGTAAG+TGG - chr3.4:83440154-83440173 MS.gene013617:CDS 40.0%
TCTGGGCACTTTATAGAAAC+TGG - chr3.4:83441736-83441755 MS.gene013617:CDS 40.0%
TCTTTACCTGTCACATCTGA+TGG - chr3.4:83441324-83441343 MS.gene013617:CDS 40.0%
TGAATTGGGCCCAATAGATT+GGG - chr3.4:83441569-83441588 MS.gene013617:CDS 40.0%
! AAGGTTTCAGCCTTTTTCTG+TGG + chr3.4:83440535-83440554 None:intergenic 40.0%
! AATCATTTTGCTACGGGATC+TGG - chr3.4:83440991-83441010 MS.gene013617:CDS 40.0%
! ACAACTGGTTCAGAGAAGTA+TGG - chr3.4:83441261-83441280 MS.gene013617:CDS 40.0%
! ACATGTTGATGGATAAGGCA+CGG - chr3.4:83441142-83441161 MS.gene013617:CDS 40.0%
! ATAGAAACTGGTCTCCTGAT+TGG - chr3.4:83441748-83441767 MS.gene013617:CDS 40.0%
! CATTTTCACACAAGGTTAGG+TGG - chr3.4:83441664-83441683 MS.gene013617:CDS 40.0%
! CCACAAAGCCATTTAGAATG+CGG - chr3.4:83441517-83441536 MS.gene013617:CDS 40.0%
! CGGAGAGGAAATTTTCTGAA+AGG - chr3.4:83440032-83440051 MS.gene013617:CDS 40.0%
! GAAAGGACTTGTGGTTTTTC+AGG - chr3.4:83441066-83441085 MS.gene013617:CDS 40.0%
! TTTCAGCCTTTTTCTGTGGA+GGG + chr3.4:83440531-83440550 None:intergenic 40.0%
!! AGTGGAGAAATGGATTGGTA+TGG - chr3.4:83440172-83440191 MS.gene013617:CDS 40.0%
!! GCTTTAGGACAGTATTTCGA+AGG - chr3.4:83441880-83441899 MS.gene013617:CDS 40.0%
!! TGAATTCTGGTGCTGTTGAT+GGG + chr3.4:83440707-83440726 None:intergenic 40.0%
!!! ACTTGTGGTTTTTCAGGTCT+TGG - chr3.4:83441072-83441091 MS.gene013617:CDS 40.0%
!!! ACTTGTTTTTGTCAGGGTGA+AGG + chr3.4:83440267-83440286 None:intergenic 40.0%
!!! CTTGTTTTTGTCAGGGTGAA+GGG + chr3.4:83440266-83440285 None:intergenic 40.0%
ACTCCCCTTGTTAAGTTTCC+TGG - chr3.4:83441858-83441877 MS.gene013617:CDS 45.0%
ACTGTACCATCAGATGTGAC+AGG + chr3.4:83441333-83441352 None:intergenic 45.0%
AGCCTTTCAAACTCCTCTTC+TGG + chr3.4:83440372-83440391 None:intergenic 45.0%
AGCTGAAAGTTCCTTTGCAG+AGG - chr3.4:83441422-83441441 MS.gene013617:CDS 45.0%
AGGAACTTTCAGCTCTATCC+AGG + chr3.4:83441416-83441435 None:intergenic 45.0%
ATACTACTCCAAACCGTGCT+GGG - chr3.4:83440398-83440417 MS.gene013617:CDS 45.0%
ATTCGCGTTAATGGCTATCC+AGG - chr3.4:83440958-83440977 MS.gene013617:CDS 45.0%
CAACAAAGATGAGGCAGTCA+GGG - chr3.4:83439997-83440016 MS.gene013617:CDS 45.0%
CACAAGGTTAGGTGGACAAA+AGG - chr3.4:83441672-83441691 MS.gene013617:CDS 45.0%
CAGTTTCTCTGGATCAGGAA+AGG - chr3.4:83441029-83441048 MS.gene013617:CDS 45.0%
CATCAATTGCTGAATGGGAG+AGG - chr3.4:83440881-83440900 MS.gene013617:CDS 45.0%
CCAATAGATTGGGTAGGTTC+TGG - chr3.4:83441579-83441598 MS.gene013617:CDS 45.0%
CCAGAACCTACCCAATCTAT+TGG + chr3.4:83441582-83441601 None:intergenic 45.0%
CGAAATACTGTCCTAAAGCC+AGG + chr3.4:83441879-83441898 None:intergenic 45.0%
CTCTCCCATTCAGCAATTGA+TGG + chr3.4:83440883-83440902 None:intergenic 45.0%
CTCTCGAAGCAACAGTGAAT+TGG - chr3.4:83441554-83441573 MS.gene013617:CDS 45.0%
CTTCTGTTGCTATCTGAAGG+AGG + chr3.4:83442055-83442074 None:intergenic 45.0%
GAAGCTCATAACGCTCCAAT+AGG - chr3.4:83441993-83442012 MS.gene013617:CDS 45.0%
GAATCCTCCTAACCTCATTC+TGG + chr3.4:83441914-83441933 None:intergenic 45.0%
GAGCACCATCAATTGCTGAA+TGG - chr3.4:83440875-83440894 MS.gene013617:CDS 45.0%
GATGGAGTGCAACAAAGATG+AGG - chr3.4:83439988-83440007 MS.gene013617:CDS 45.0%
GCAACTTCAAATGTGAGGTC+TGG - chr3.4:83440472-83440491 MS.gene013617:CDS 45.0%
GCTATCTGAAGGAGGTCTAA+AGG + chr3.4:83442047-83442066 None:intergenic 45.0%
GCTGCTTATGACGATGATGA+TGG - chr3.4:83441456-83441475 MS.gene013617:CDS 45.0%
GGCTTAAAAACATTGGGAGG+AGG + chr3.4:83440670-83440689 None:intergenic 45.0%
GGTCTGGGAATCATAATGCT+CGG - chr3.4:83440488-83440507 MS.gene013617:CDS 45.0%
GGTTAGGAGGATTCCTAAAG+AGG - chr3.4:83441920-83441939 MS.gene013617:CDS 45.0%
GTCTGGGAATCATAATGCTC+GGG - chr3.4:83440489-83440508 MS.gene013617:CDS 45.0%
GTGAATTGGGCCCAATAGAT+TGG - chr3.4:83441568-83441587 MS.gene013617:CDS 45.0%
GTTCCTAATCACTTGCTCAG+TGG - chr3.4:83441966-83441985 MS.gene013617:CDS 45.0%
TGGATCAGGAAAGGCTAACT+TGG - chr3.4:83441038-83441057 MS.gene013617:CDS 45.0%
! AAGCTTTTGTGGCCATAGAG+AGG - chr3.4:83440641-83440660 MS.gene013617:CDS 45.0%
! CACGGTTTGGAGTAGTATGT+TGG + chr3.4:83440396-83440415 None:intergenic 45.0%
! CCGCATTCTAAATGGCTTTG+TGG + chr3.4:83441520-83441539 None:intergenic 45.0%
! GTTTCAGCCTTTTTCTGTGG+AGG + chr3.4:83440532-83440551 None:intergenic 45.0%
! TGCATTTCAGCTGGTTTCAG+AGG - chr3.4:83440303-83440322 MS.gene013617:CDS 45.0%
! TTCAGCCTTTTTCTGTGGAG+GGG + chr3.4:83440530-83440549 None:intergenic 45.0%
!! AAAACCAGCCATTCTGGAGT+GGG + chr3.4:83440773-83440792 None:intergenic 45.0%
!! GTGAATTCTGGTGCTGTTGA+TGG + chr3.4:83440708-83440727 None:intergenic 45.0%
!!! TGTGGTTTTTCAGGTCTTGG+AGG - chr3.4:83441075-83441094 MS.gene013617:CDS 45.0%
AAAGATGCGGACAACTCCTC+TGG + chr3.4:83441700-83441719 None:intergenic 50.0%
AAGTTCCTTTGCAGAGGACC+AGG - chr3.4:83441428-83441447 MS.gene013617:CDS 50.0%
ACAGAAGCTAATGAGGCAGC+AGG - chr3.4:83442068-83442087 MS.gene013617:CDS 50.0%
ACCAGGACCAGAATGAGGTT+AGG - chr3.4:83441904-83441923 MS.gene013617:CDS 50.0%
ACTTGTGGGGATTTCAGGAC+TGG - chr3.4:83441612-83441631 MS.gene013617:CDS 50.0%
AGGACCAGAATGAGGTTAGG+AGG - chr3.4:83441907-83441926 MS.gene013617:CDS 50.0%
CAGAATTCACGACGTCATCC+TGG - chr3.4:83440718-83440737 MS.gene013617:CDS 50.0%
CAGGATGACGTCGTGAATTC+TGG + chr3.4:83440720-83440739 None:intergenic 50.0%
CATACTACTCCAAACCGTGC+TGG - chr3.4:83440397-83440416 MS.gene013617:CDS 50.0%
GCAACAAAGATGAGGCAGTC+AGG - chr3.4:83439996-83440015 MS.gene013617:CDS 50.0%
GCTACCAAAACCAGCCATTC+TGG + chr3.4:83440779-83440798 None:intergenic 50.0%
GTCCGCATCTTTCCAGGTAA+GGG - chr3.4:83441708-83441727 MS.gene013617:CDS 50.0%
TAATCACTTGCTCAGTGGCC+AGG - chr3.4:83441971-83441990 MS.gene013617:CDS 50.0%
TAGCTGCTGGATCCAACTCT+CGG + chr3.4:83442022-83442041 None:intergenic 50.0%
TCCAACTCTCGGCAACCTAT+TGG + chr3.4:83442011-83442030 None:intergenic 50.0%
TCCAATAGGTTGCCGAGAGT+TGG - chr3.4:83442007-83442026 MS.gene013617:CDS 50.0%
TCCGCATCTTTCCAGGTAAG+GGG - chr3.4:83441709-83441728 MS.gene013617:CDS 50.0%
TCCTAACCTCATTCTGGTCC+TGG + chr3.4:83441908-83441927 None:intergenic 50.0%
TGATGGGAGGGCAAACTTGA+TGG + chr3.4:83440691-83440710 None:intergenic 50.0%
TGGCCACTGAGCAAGTGATT+AGG + chr3.4:83441972-83441991 None:intergenic 50.0%
TGTCCGCATCTTTCCAGGTA+AGG - chr3.4:83441707-83441726 MS.gene013617:CDS 50.0%
TTCTGCCCCTCCACAGAAAA+AGG - chr3.4:83440522-83440541 MS.gene013617:CDS 50.0%
TTGGGCAGTTTCTCTGGATC+AGG - chr3.4:83441024-83441043 MS.gene013617:CDS 50.0%
TTGGGCCCAATAGATTGGGT+AGG - chr3.4:83441573-83441592 MS.gene013617:CDS 50.0%
! AGGTCTAAAGGTGTAGCTGC+TGG + chr3.4:83442035-83442054 None:intergenic 50.0%
! CAAAACCAGCCATTCTGGAG+TGG + chr3.4:83440774-83440793 None:intergenic 50.0%
! CATTTGATGGATGCCCAGCA+CGG + chr3.4:83440414-83440433 None:intergenic 50.0%
! GGGCTAAAGAAATAGCGGAG+AGG - chr3.4:83440017-83440036 MS.gene013617:CDS 50.0%
! TTCAGCTGGTTTCAGAGGCA+TGG - chr3.4:83440308-83440327 MS.gene013617:CDS 50.0%
! TTGGAGCGTTATGAGCTTCC+TGG + chr3.4:83441992-83442011 None:intergenic 50.0%
!! ATTCTGGTGCTGTTGATGGG+AGG + chr3.4:83440704-83440723 None:intergenic 50.0%
!! GACAGTATTTCGAAGGCACC+AGG - chr3.4:83441887-83441906 MS.gene013617:CDS 50.0%
!! TTCTGGTGCTGTTGATGGGA+GGG + chr3.4:83440703-83440722 None:intergenic 50.0%
!!! CACTCCAGAATGGCTGGTTT+TGG - chr3.4:83440772-83440791 MS.gene013617:CDS 50.0%
CACGACACTTGCAGCTTGAG+CGG + chr3.4:83440824-83440843 None:intergenic 55.0%
CCCCCTTACCTGGAAAGATG+CGG + chr3.4:83441713-83441732 None:intergenic 55.0%
CCGCATCTTTCCAGGTAAGG+GGG - chr3.4:83441710-83441729 MS.gene013617:CDS 55.0%
CTTTGCAGAGGACCAGGTCT+GGG - chr3.4:83441434-83441453 MS.gene013617:CDS 55.0%
GCAGGTTTGGGCAGTTTCTC+TGG - chr3.4:83441018-83441037 MS.gene013617:CDS 55.0%
GGAGTTGTCCGCATCTTTCC+AGG - chr3.4:83441702-83441721 MS.gene013617:CDS 55.0%
GGCAACAGACGACGATCCAT+CGG + chr3.4:83440803-83440822 None:intergenic 55.0%
TCCAGGTAAGGGGGAAGTCT+GGG - chr3.4:83441719-83441738 MS.gene013617:CDS 55.0%
TTCCAGGTAAGGGGGAAGTC+TGG - chr3.4:83441718-83441737 MS.gene013617:CDS 55.0%
TTTCTGTGGAGGGGCAGAAG+TGG + chr3.4:83440521-83440540 None:intergenic 55.0%
! AAGGCACCAGGACCAGAATG+AGG - chr3.4:83441899-83441918 MS.gene013617:CDS 55.0%
! AGCTGCAAGTGTCGTGCAGA+AGG - chr3.4:83440828-83440847 MS.gene013617:CDS 55.0%
! GGATCTGGAGCAGCAGGTTT+GGG - chr3.4:83441006-83441025 MS.gene013617:CDS 55.0%
! TGCAGAGGGTGCATTTCAGC+TGG - chr3.4:83440294-83440313 MS.gene013617:CDS 55.0%
!! AGGTGGACAAAAGGCACCAG+AGG - chr3.4:83441681-83441700 MS.gene013617:CDS 55.0%
!!! GCTGGTTTTGGTAGCACCGA+TGG - chr3.4:83440784-83440803 MS.gene013617:CDS 55.0%
CCAGACCTGGTCCTCTGCAA+AGG + chr3.4:83441436-83441455 None:intergenic 60.0%
CCTTTGCAGAGGACCAGGTC+TGG - chr3.4:83441433-83441452 MS.gene013617:CDS 60.0%
CGTCATAAGCAGCCCAGACC+TGG + chr3.4:83441449-83441468 None:intergenic 60.0%
GATGGATGCCCAGCACGGTT+TGG + chr3.4:83440409-83440428 None:intergenic 60.0%
GGGAGGAGGACTCCTCTCTA+TGG + chr3.4:83440656-83440675 None:intergenic 60.0%
GGTTCCCACTCCAGAATGGC+TGG - chr3.4:83440766-83440785 MS.gene013617:CDS 60.0%
GTGGGGTTCCCACTCCAGAA+TGG - chr3.4:83440762-83440781 MS.gene013617:CDS 60.0%
TGGAGTGGGAACCCCACTGA+AGG + chr3.4:83440759-83440778 None:intergenic 60.0%
! GGGATCTGGAGCAGCAGGTT+TGG - chr3.4:83441005-83441024 MS.gene013617:CDS 60.0%
GCCCAGACTTCCCCCTTACC+TGG + chr3.4:83441723-83441742 None:intergenic 65.0%
TGTGGAGGGGCAGAAGTGGC+AGG + chr3.4:83440517-83440536 None:intergenic 65.0%
! GCTACGGGATCTGGAGCAGC+AGG - chr3.4:83441000-83441019 MS.gene013617:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 83439986 83442100 83439986 ID=MS.gene013617
chr3.4 mRNA 83439986 83442100 83439986 ID=MS.gene013617.t1;Parent=MS.gene013617
chr3.4 exon 83439986 83442100 83439986 ID=MS.gene013617.t1.exon1;Parent=MS.gene013617.t1
chr3.4 CDS 83439986 83442100 83439986 ID=cds.MS.gene013617.t1;Parent=MS.gene013617.t1
Gene Sequence

>MS.gene013617

ATGATGGAGTGCAACAAAGATGAGGCAGTCAGGGCTAAAGAAATAGCGGAGAGGAAATTTTCTGAAAGGGAATATATTGGTGCAAAGAAGTTTGCTATCAAGGCTAAAAATTTGTATGCTGATTTGGAGGATATTTCCCAGTTTTTGACTACTATTGATATTTATATATCTGCTGAGAACAAAGTAAGTGGAGAAATGGATTGGTATGGTATACTTGGAGTGAGTCCCTTTGCTGATGAAGAGACTGTTAGAAAGCAGTACAGGAAACTGGCTCTCACCCTTCACCCTGACAAAAACAAGTCTTTAGGTGCAGAGGGTGCATTTCAGCTGGTTTCAGAGGCATGGAGTTTGTTATCAGATAAGACCAAGAGACTAGAATATAACCAGAAGAGGAGTTTGAAAGGCTTCCAACATACTACTCCAAACCGTGCTGGGCATCCATCAAATGTACCTAGTTCAAATGGTTATTATCATTTTAAGAAGAATGCAACTTCAAATGTGAGGTCTGGGAATCATAATGCTCGGGCTCCTGCCACTTCTGCCCCTCCACAGAAAAAGGCTGAAACCTTTTGGACTATCTGTAATAAGTGCAGGACACATTATGAATATCTCAGAGTTTATTTGAATCACACTCTTCTATGTCCCAACTGTAATGAAGCTTTTGTGGCCATAGAGAGGAGTCCTCCTCCCAATGTTTTTAAGCCATCAAGTTTGCCCTCCCATCAACAGCACCAGAATTCACGACGTCATCCTGGAAGTAATAATTCAAACCTTCAGTGGGGTTCCCACTCCAGAATGGCTGGTTTTGGTAGCACCGATGGATCGTCGTCTGTTGCCGCTCAAGCTGCAAGTGTCGTGCAGAAGGCAAGTGAGAAAGTGAAGAGAGAAGGAGCACCATCAATTGCTGAATGGGAGAGGATTCAAATGTCTAAGAAGGCTGATGGTTCTATGAAGAAAAGGAAGGCAGATGATATTCGCGTTAATGGCTATCCAGGATATACGGTAAATCATTTTGCTACGGGATCTGGAGCAGCAGGTTTGGGCAGTTTCTCTGGATCAGGAAAGGCTAACTTGGAAAAAGAAAGGACTTGTGGTTTTTCAGGTCTTGGAGGCAAACATTACAGCATACGAGAGTTGTCATTGTTTGAGTTACGAAACATGTTGATGGATAAGGCACGGAATGAAATTAGTAAAAAACTTGAAGAATGGAGGTTAATGGCTGAAGCTAAGACTGTCAACAAGAATAAGAGACAGAAAAACATATTCAATGACAAAACAACTGGTTCAGAGAAGTATGGAGAGTCCAATGTTAATGGTAACAAGCATTTTTCTGTTGATTCTTTACCTGTCACATCTGATGGTACAGTTAAGAAGAGCCAAGCCTATGTTACAATCAATGTTCCGGATCCTGATTTTCACAATTTTGACCTGGATAGAGCTGAAAGTTCCTTTGCAGAGGACCAGGTCTGGGCTGCTTATGACGATGATGATGGAATGCCTAGATATTATGCTAGAATTCACAAGGTGATCTCCACAAAGCCATTTAGAATGCGGATTAGTTGGCTTAACTCTCGAAGCAACAGTGAATTGGGCCCAATAGATTGGGTAGGTTCTGGTTTTTATAAAACTTGTGGGGATTTCAGGACTGGCAAGCATGAAGTATCTGAATCATTAAATTCATTTTCACACAAGGTTAGGTGGACAAAAGGCACCAGAGGAGTTGTCCGCATCTTTCCAGGTAAGGGGGAAGTCTGGGCACTTTATAGAAACTGGTCTCCTGATTGGAATGAACATACTCCAGATGAAGTGATACACAAGTATGACATGGTGGAAGTGCTTGATGATTTCAGTGAAGAGCAAGGTATATTAGTTACTCCCCTTGTTAAGTTTCCTGGCTTTAGGACAGTATTTCGAAGGCACCAGGACCAGAATGAGGTTAGGAGGATTCCTAAAGAGGAGATGTTTCGATTCTCTCATCAGGTTCCTAATCACTTGCTCAGTGGCCAGGAAGCTCATAACGCTCCAATAGGTTGCCGAGAGTTGGATCCAGCAGCTACACCTTTAGACCTCCTTCAGATAGCAACAGAAGCTAATGAGGCAGCAGGTAATGTTTAG
Protein sequence

>MS.gene013617.t1

MMECNKDEAVRAKEIAERKFSEREYIGAKKFAIKAKNLYADLEDISQFLTTIDIYISAENKVSGEMDWYGILGVSPFADEETVRKQYRKLALTLHPDKNKSLGAEGAFQLVSEAWSLLSDKTKRLEYNQKRSLKGFQHTTPNRAGHPSNVPSSNGYYHFKKNATSNVRSGNHNARAPATSAPPQKKAETFWTICNKCRTHYEYLRVYLNHTLLCPNCNEAFVAIERSPPPNVFKPSSLPSHQQHQNSRRHPGSNNSNLQWGSHSRMAGFGSTDGSSSVAAQAASVVQKASEKVKREGAPSIAEWERIQMSKKADGSMKKRKADDIRVNGYPGYTVNHFATGSGAAGLGSFSGSGKANLEKERTCGFSGLGGKHYSIRELSLFELRNMLMDKARNEISKKLEEWRLMAEAKTVNKNKRQKNIFNDKTTGSEKYGESNVNGNKHFSVDSLPVTSDGTVKKSQAYVTINVPDPDFHNFDLDRAESSFAEDQVWAAYDDDDGMPRYYARIHKVISTKPFRMRISWLNSRSNSELGPIDWVGSGFYKTCGDFRTGKHEVSESLNSFSHKVRWTKGTRGVVRIFPGKGEVWALYRNWSPDWNEHTPDEVIHKYDMVEVLDDFSEEQGILVTPLVKFPGFRTVFRRHQDQNEVRRIPKEEMFRFSHQVPNHLLSGQEAHNAPIGCRELDPAATPLDLLQIATEANEAAGNV