Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014911.t1 | XP_003603289.1 | 82.6 | 178 | 8 | 1 | 1 | 155 | 1 | 178 | 6.40E-70 | 273.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014911.t1 | Q9SK27 | 46.2 | 169 | 65 | 3 | 5 | 149 | 9 | 175 | 5.0e-31 | 135.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014911.t1 | G7J9U0 | 82.6 | 178 | 8 | 1 | 1 | 155 | 1 | 178 | 4.6e-70 | 273.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene006553 | MS.gene014911 | PPI |
| MS.gene031754 | MS.gene014911 | PPI |
| MS.gene065888 | MS.gene014911 | PPI |
| MS.gene038783 | MS.gene014911 | PPI |
| MS.gene032997 | MS.gene014911 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014911.t1 | MTR_3g105930 | 82.584 | 178 | 8 | 1 | 1 | 155 | 1 | 178 | 2.22e-86 | 251 |
| MS.gene014911.t1 | MTR_7g090170 | 89.189 | 148 | 10 | 1 | 1 | 142 | 1 | 148 | 4.29e-77 | 226 |
| MS.gene014911.t1 | MTR_1g014120 | 54.483 | 145 | 39 | 3 | 7 | 124 | 4 | 148 | 2.12e-47 | 152 |
| MS.gene014911.t1 | MTR_4g077787 | 33.333 | 183 | 84 | 6 | 1 | 153 | 1 | 175 | 7.85e-21 | 84.3 |
| MS.gene014911.t1 | MTR_2g101300 | 36.029 | 136 | 60 | 5 | 23 | 134 | 23 | 155 | 5.47e-20 | 82.4 |
| MS.gene014911.t1 | MTR_4g081100 | 36.364 | 121 | 53 | 2 | 11 | 108 | 16 | 135 | 6.37e-19 | 80.5 |
| MS.gene014911.t1 | MTR_4g111640 | 31.492 | 181 | 85 | 8 | 8 | 158 | 7 | 178 | 8.38e-19 | 79.0 |
| MS.gene014911.t1 | MTR_2g079030 | 37.415 | 147 | 63 | 4 | 12 | 132 | 10 | 153 | 1.10e-18 | 82.4 |
| MS.gene014911.t1 | MTR_8g463180 | 37.037 | 135 | 53 | 5 | 12 | 119 | 5 | 134 | 1.35e-18 | 80.9 |
| MS.gene014911.t1 | MTR_6g022170 | 40.952 | 105 | 39 | 3 | 23 | 105 | 30 | 133 | 1.42e-17 | 75.5 |
| MS.gene014911.t1 | MTR_2g083250 | 40.741 | 108 | 38 | 4 | 24 | 106 | 23 | 129 | 7.47e-17 | 75.5 |
| MS.gene014911.t1 | MTR_2g090580 | 33.951 | 162 | 69 | 6 | 10 | 136 | 2 | 160 | 2.97e-16 | 72.8 |
| MS.gene014911.t1 | MTR_4g130780 | 35.398 | 113 | 50 | 2 | 15 | 104 | 17 | 129 | 2.74e-15 | 69.7 |
| MS.gene014911.t1 | MTR_4g112310 | 28.571 | 133 | 67 | 3 | 47 | 158 | 3 | 128 | 2.07e-14 | 66.2 |
| MS.gene014911.t1 | MTR_4g078410 | 32.738 | 168 | 73 | 5 | 24 | 155 | 22 | 185 | 4.47e-14 | 66.6 |
| MS.gene014911.t1 | MTR_4g130800 | 34.167 | 120 | 53 | 3 | 12 | 105 | 11 | 130 | 6.39e-13 | 65.1 |
| MS.gene014911.t1 | MTR_2g088990 | 30.612 | 147 | 72 | 5 | 14 | 134 | 12 | 154 | 4.41e-12 | 62.0 |
| MS.gene014911.t1 | MTR_2g090575 | 32.184 | 174 | 77 | 7 | 12 | 150 | 7 | 174 | 1.20e-11 | 62.0 |
| MS.gene014911.t1 | MTR_8g094990 | 31.481 | 162 | 68 | 5 | 14 | 134 | 12 | 171 | 3.97e-11 | 60.1 |
| MS.gene014911.t1 | MTR_8g086360 | 32.836 | 134 | 56 | 5 | 23 | 133 | 20 | 142 | 6.97e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014911.t1 | AT2G25060 | 47.239 | 163 | 60 | 3 | 11 | 149 | 15 | 175 | 6.72e-43 | 140 |
| MS.gene014911.t1 | AT4G31840 | 45.614 | 171 | 61 | 5 | 8 | 149 | 2 | 169 | 1.77e-39 | 132 |
| MS.gene014911.t1 | AT5G25090 | 37.222 | 180 | 79 | 4 | 6 | 151 | 4 | 183 | 1.06e-30 | 110 |
| MS.gene014911.t1 | AT5G57920 | 44.628 | 121 | 43 | 3 | 22 | 118 | 18 | 138 | 8.35e-30 | 107 |
| MS.gene014911.t1 | AT5G57920 | 44.628 | 121 | 43 | 3 | 22 | 118 | 50 | 170 | 1.49e-29 | 107 |
| MS.gene014911.t1 | AT5G57920 | 43.200 | 125 | 47 | 3 | 18 | 118 | 13 | 137 | 2.10e-29 | 106 |
| MS.gene014911.t1 | AT4G30590 | 41.270 | 126 | 46 | 2 | 12 | 109 | 8 | 133 | 2.36e-28 | 103 |
| MS.gene014911.t1 | AT2G23990 | 39.744 | 156 | 58 | 4 | 1 | 132 | 1 | 144 | 4.91e-26 | 98.6 |
| MS.gene014911.t1 | AT2G23990 | 36.416 | 173 | 53 | 5 | 5 | 132 | 3 | 163 | 2.84e-23 | 92.0 |
| MS.gene014911.t1 | AT1G48940 | 36.145 | 166 | 81 | 5 | 11 | 153 | 10 | 173 | 5.14e-23 | 90.1 |
| MS.gene014911.t1 | AT3G18590 | 42.735 | 117 | 43 | 3 | 12 | 105 | 12 | 127 | 1.40e-22 | 89.0 |
| MS.gene014911.t1 | AT3G20570 | 29.070 | 172 | 70 | 4 | 12 | 133 | 15 | 184 | 1.80e-18 | 79.0 |
| MS.gene014911.t1 | AT5G53870 | 34.868 | 152 | 59 | 6 | 11 | 132 | 7 | 148 | 2.14e-16 | 75.5 |
| MS.gene014911.t1 | AT5G53870 | 34.868 | 152 | 59 | 6 | 11 | 132 | 7 | 148 | 2.29e-16 | 75.5 |
| MS.gene014911.t1 | AT4G27520 | 35.156 | 128 | 57 | 3 | 12 | 116 | 16 | 140 | 1.30e-15 | 73.2 |
| MS.gene014911.t1 | AT5G14345 | 35.455 | 110 | 49 | 3 | 36 | 123 | 24 | 133 | 1.47e-14 | 67.0 |
| MS.gene014911.t1 | AT1G79800 | 33.981 | 103 | 45 | 3 | 25 | 105 | 32 | 133 | 4.45e-14 | 67.0 |
| MS.gene014911.t1 | AT4G28365 | 30.380 | 158 | 73 | 7 | 12 | 135 | 11 | 165 | 3.17e-11 | 59.3 |
| MS.gene014911.t1 | AT4G32490 | 30.657 | 137 | 64 | 5 | 3 | 111 | 2 | 135 | 5.92e-11 | 58.9 |
Find 46 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTGGACTTGTGACTGTTC+TGG | 0.274010 | 3.1:-83750764 | MS.gene014911:CDS |
| TTGTTCTTGTTCCTTCTCTT+TGG | 0.278533 | 3.1:-83751310 | MS.gene014911:CDS |
| CCATCATTGTAATGTTCAAA+TGG | 0.356138 | 3.1:+83750988 | None:intergenic |
| CATCATTGTAATGTTCAAAT+GGG | 0.374864 | 3.1:+83750989 | None:intergenic |
| GGGACTGTTGGCTATTTATG+TGG | 0.381502 | 3.1:-83750743 | MS.gene014911:CDS |
| GCTCCACTGATGAAGTAAAA+TGG | 0.384841 | 3.1:+83750934 | None:intergenic |
| ACTGGACTTGTGACTGTTCT+GGG | 0.401738 | 3.1:-83750763 | MS.gene014911:CDS |
| CTCTGCAGCTAAGGAACTAT+TGG | 0.408413 | 3.1:-83751284 | MS.gene014911:CDS |
| CCATTTGAACATTACAATGA+TGG | 0.423646 | 3.1:-83750988 | MS.gene014911:CDS |
| TTCAAAGTCGGCGATCATCT+TGG | 0.440071 | 3.1:-83751184 | MS.gene014911:intron |
| TACTTCATCAGTGGAGCAAA+GGG | 0.460490 | 3.1:-83750928 | MS.gene014911:CDS |
| GGACCTTCTTCCAACTCTGC+TGG | 0.465938 | 3.1:+83750823 | None:intergenic |
| AGTGCGCCGGTGTTGCGAAC+TGG | 0.466338 | 3.1:-83750781 | MS.gene014911:CDS |
| CTTTGGTTTCTCTGCAGCTA+AGG | 0.473136 | 3.1:-83751293 | MS.gene014911:CDS |
| CTGCTAGTTGGTGCAACAGC+TGG | 0.474292 | 3.1:+83750802 | None:intergenic |
| GGACTGTTGGCTATTTATGT+GGG | 0.487905 | 3.1:-83750742 | MS.gene014911:CDS |
| AGTGAGTCTGCTTCAGATGA+TGG | 0.493397 | 3.1:+83751232 | None:intergenic |
| CATTTGAACATTACAATGAT+GGG | 0.496988 | 3.1:-83750987 | MS.gene014911:CDS |
| TGCACCATCTCCAGCAGAGT+TGG | 0.499713 | 3.1:-83750833 | MS.gene014911:CDS |
| TCTTCCAACTCTGCTGGAGA+TGG | 0.500018 | 3.1:+83750829 | None:intergenic |
| TGTGACTGTTCTGGGACTGT+TGG | 0.507080 | 3.1:-83750755 | MS.gene014911:CDS |
| AACTCTGCTGGAGATGGTGC+AGG | 0.510903 | 3.1:+83750835 | None:intergenic |
| AGCAGACTCACTCAACCAAT+GGG | 0.514967 | 3.1:-83751221 | MS.gene014911:CDS |
| AACACCGGCGCACTGCTAGT+TGG | 0.529681 | 3.1:+83750790 | None:intergenic |
| TCTCCAAAGAAAAGATCCAT+TGG | 0.532263 | 3.1:-83750865 | MS.gene014911:CDS |
| TTACTTCATCAGTGGAGCAA+AGG | 0.544859 | 3.1:-83750929 | MS.gene014911:CDS |
| AAGCAGACTCACTCAACCAA+TGG | 0.548958 | 3.1:-83751222 | MS.gene014911:CDS |
| GCAAAGGGACACTGTGAGCA+AGG | 0.567702 | 3.1:-83750913 | MS.gene014911:CDS |
| CTGATGAAGTAAAATGGTCC+AGG | 0.572076 | 3.1:+83750940 | None:intergenic |
| TGCACCAACTAGCAGTGCGC+CGG | 0.584450 | 3.1:-83750794 | MS.gene014911:CDS |
| GAAAAGTCTCGTTTCAAAGT+CGG | 0.587560 | 3.1:-83751196 | MS.gene014911:CDS |
| TCTCCAGCAGAGTTGGAAGA+AGG | 0.588093 | 3.1:-83750826 | MS.gene014911:CDS |
| ACAAGTCCAGTTCGCAACAC+CGG | 0.589779 | 3.1:+83750775 | None:intergenic |
| ATCATTGTAATGTTCAAATG+GGG | 0.593192 | 3.1:+83750990 | None:intergenic |
| TGGTGCAGGTGAAACTCCAA+TGG | 0.600203 | 3.1:+83750849 | None:intergenic |
| CACTATATCCTAATTAGGTG+CGG | 0.608986 | 3.1:+83750701 | None:intergenic |
| AGGCAAGATAGATGCATGGA+AGG | 0.615286 | 3.1:-83751257 | MS.gene014911:CDS |
| CTGCAGAGAAACCAAAGAGA+AGG | 0.616684 | 3.1:+83751299 | None:intergenic |
| AAGGTAAAGCTAGACAGACC+TGG | 0.622619 | 3.1:-83750958 | MS.gene014911:CDS |
| GCAGCTAAGGAACTATTGGT+CGG | 0.634929 | 3.1:-83751280 | MS.gene014911:CDS |
| AGTTCTTGAGAACACAGCCA+TGG | 0.674329 | 3.1:+83751348 | None:intergenic |
| TCGGAGGCAAGATAGATGCA+TGG | 0.675253 | 3.1:-83751261 | MS.gene014911:CDS |
| AGGACAAAAGCTGATTGTGG+TGG | 0.679977 | 3.1:-83750893 | MS.gene014911:CDS |
| GCTAAGGAACTATTGGTCGG+AGG | 0.690687 | 3.1:-83751277 | MS.gene014911:CDS |
| GCAAGGACAAAAGCTGATTG+TGG | 0.718198 | 3.1:-83750896 | MS.gene014911:CDS |
| TTACAATGATGGGAACACGA+AGG | 0.756088 | 3.1:-83750977 | MS.gene014911:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCATTGTAATGTTCAAATG+GGG | + | chr3.1:83751073-83751092 | None:intergenic | 25.0% |
| ! | CATCATTGTAATGTTCAAAT+GGG | + | chr3.1:83751074-83751093 | None:intergenic | 25.0% |
| ! | CATTTGAACATTACAATGAT+GGG | - | chr3.1:83751073-83751092 | MS.gene014911:intron | 25.0% |
| ATTTCCAAACTGCAAAACAA+AGG | + | chr3.1:83750982-83751001 | None:intergenic | 30.0% | |
| CCATCATTGTAATGTTCAAA+TGG | + | chr3.1:83751075-83751094 | None:intergenic | 30.0% | |
| CCATTTGAACATTACAATGA+TGG | - | chr3.1:83751072-83751091 | MS.gene014911:intron | 30.0% | |
| TTTGCAGTTTGGAAATATGA+TGG | - | chr3.1:83750986-83751005 | MS.gene014911:CDS | 30.0% | |
| !!! | TTTACCTTTGTTTTGCAGTT+TGG | - | chr3.1:83750975-83750994 | MS.gene014911:CDS | 30.0% |
| AGAAAAAGATGTGTCAGTGA+TGG | + | chr3.1:83750955-83750974 | None:intergenic | 35.0% | |
| CAGTTTGGAAATATGATGGT+GGG | - | chr3.1:83750990-83751009 | MS.gene014911:CDS | 35.0% | |
| GAAAAGTCTCGTTTCAAAGT+CGG | - | chr3.1:83750864-83750883 | MS.gene014911:CDS | 35.0% | |
| TCTCCAAAGAAAAGATCCAT+TGG | - | chr3.1:83751195-83751214 | MS.gene014911:CDS | 35.0% | |
| TTGTTCTTGTTCCTTCTCTT+TGG | - | chr3.1:83750750-83750769 | MS.gene014911:CDS | 35.0% | |
| ! | ACTCCAATGGATCTTTTCTT+TGG | + | chr3.1:83751201-83751220 | None:intergenic | 35.0% |
| ! | AGTTTGGAAATATGATGGTG+GGG | - | chr3.1:83750991-83751010 | MS.gene014911:CDS | 35.0% |
| CTGATGAAGTAAAATGGTCC+AGG | + | chr3.1:83751123-83751142 | None:intergenic | 40.0% | |
| GCAGTTTGGAAATATGATGG+TGG | - | chr3.1:83750989-83751008 | MS.gene014911:CDS | 40.0% | |
| GCTCCACTGATGAAGTAAAA+TGG | + | chr3.1:83751129-83751148 | None:intergenic | 40.0% | |
| TACTTCATCAGTGGAGCAAA+GGG | - | chr3.1:83751132-83751151 | MS.gene014911:intron | 40.0% | |
| TTACTTCATCAGTGGAGCAA+AGG | - | chr3.1:83751131-83751150 | MS.gene014911:intron | 40.0% | |
| ! | GGACCATTTTACTTCATCAG+TGG | - | chr3.1:83751123-83751142 | MS.gene014911:intron | 40.0% |
| ! | GGACTGTTGGCTATTTATGT+GGG | - | chr3.1:83751318-83751337 | MS.gene014911:CDS | 40.0% |
| ! | TTACAATGATGGGAACACGA+AGG | - | chr3.1:83751083-83751102 | MS.gene014911:intron | 40.0% |
| AACTGGACTTGTGACTGTTC+TGG | - | chr3.1:83751296-83751315 | MS.gene014911:CDS | 45.0% | |
| AAGCAGACTCACTCAACCAA+TGG | - | chr3.1:83750838-83750857 | MS.gene014911:CDS | 45.0% | |
| AAGGTAAAGCTAGACAGACC+TGG | - | chr3.1:83751102-83751121 | MS.gene014911:intron | 45.0% | |
| ACTGGACTTGTGACTGTTCT+GGG | - | chr3.1:83751297-83751316 | MS.gene014911:CDS | 45.0% | |
| AGCAGACTCACTCAACCAAT+GGG | - | chr3.1:83750839-83750858 | MS.gene014911:CDS | 45.0% | |
| AGGACAAAAGCTGATTGTGG+TGG | - | chr3.1:83751167-83751186 | MS.gene014911:intron | 45.0% | |
| AGGCAAGATAGATGCATGGA+AGG | - | chr3.1:83750803-83750822 | MS.gene014911:CDS | 45.0% | |
| AGTGAGTCTGCTTCAGATGA+TGG | + | chr3.1:83750831-83750850 | None:intergenic | 45.0% | |
| CTCTGCAGCTAAGGAACTAT+TGG | - | chr3.1:83750776-83750795 | MS.gene014911:CDS | 45.0% | |
| CTGCAGAGAAACCAAAGAGA+AGG | + | chr3.1:83750764-83750783 | None:intergenic | 45.0% | |
| GCAAGGACAAAAGCTGATTG+TGG | - | chr3.1:83751164-83751183 | MS.gene014911:intron | 45.0% | |
| GCAGCTAAGGAACTATTGGT+CGG | - | chr3.1:83750780-83750799 | MS.gene014911:CDS | 45.0% | |
| TTCAAAGTCGGCGATCATCT+TGG | - | chr3.1:83750876-83750895 | MS.gene014911:CDS | 45.0% | |
| ! | AACGAGACTTTTCTGCCCAT+TGG | + | chr3.1:83750857-83750876 | None:intergenic | 45.0% |
| ! | CTTTGGTTTCTCTGCAGCTA+AGG | - | chr3.1:83750767-83750786 | MS.gene014911:CDS | 45.0% |
| ! | GGGACTGTTGGCTATTTATG+TGG | - | chr3.1:83751317-83751336 | MS.gene014911:CDS | 45.0% |
| ACAAGTCCAGTTCGCAACAC+CGG | + | chr3.1:83751288-83751307 | None:intergenic | 50.0% | |
| GCTAAGGAACTATTGGTCGG+AGG | - | chr3.1:83750783-83750802 | MS.gene014911:CDS | 50.0% | |
| TCGGAGGCAAGATAGATGCA+TGG | - | chr3.1:83750799-83750818 | MS.gene014911:CDS | 50.0% | |
| TCTCCAGCAGAGTTGGAAGA+AGG | - | chr3.1:83751234-83751253 | MS.gene014911:CDS | 50.0% | |
| TCTTCCAACTCTGCTGGAGA+TGG | + | chr3.1:83751234-83751253 | None:intergenic | 50.0% | |
| TGGTGCAGGTGAAACTCCAA+TGG | + | chr3.1:83751214-83751233 | None:intergenic | 50.0% | |
| !! | TGTGACTGTTCTGGGACTGT+TGG | - | chr3.1:83751305-83751324 | MS.gene014911:CDS | 50.0% |
| AACTCTGCTGGAGATGGTGC+AGG | + | chr3.1:83751228-83751247 | None:intergenic | 55.0% | |
| GCAAAGGGACACTGTGAGCA+AGG | - | chr3.1:83751147-83751166 | MS.gene014911:intron | 55.0% | |
| GGACCTTCTTCCAACTCTGC+TGG | + | chr3.1:83751240-83751259 | None:intergenic | 55.0% | |
| TGCACCATCTCCAGCAGAGT+TGG | - | chr3.1:83751227-83751246 | MS.gene014911:CDS | 55.0% | |
| ! | CTGCTAGTTGGTGCAACAGC+TGG | + | chr3.1:83751261-83751280 | None:intergenic | 55.0% |
| AACACCGGCGCACTGCTAGT+TGG | + | chr3.1:83751273-83751292 | None:intergenic | 60.0% | |
| TGCACCAACTAGCAGTGCGC+CGG | - | chr3.1:83751266-83751285 | MS.gene014911:CDS | 60.0% | |
| !! | AGTGCGCCGGTGTTGCGAAC+TGG | - | chr3.1:83751279-83751298 | MS.gene014911:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 83750714 | 83751368 | 83750714 | ID=MS.gene014911 |
| chr3.1 | mRNA | 83750714 | 83751368 | 83750714 | ID=MS.gene014911.t1;Parent=MS.gene014911 |
| chr3.1 | exon | 83751185 | 83751368 | 83751185 | ID=MS.gene014911.t1.exon1;Parent=MS.gene014911.t1 |
| chr3.1 | CDS | 83751185 | 83751368 | 83751185 | ID=cds.MS.gene014911.t1;Parent=MS.gene014911.t1 |
| chr3.1 | exon | 83750714 | 83751018 | 83750714 | ID=MS.gene014911.t1.exon2;Parent=MS.gene014911.t1 |
| chr3.1 | CDS | 83750714 | 83751018 | 83750714 | ID=cds.MS.gene014911.t1;Parent=MS.gene014911.t1 |
| Gene Sequence |
| Protein sequence |
