AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032997


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032997.t1 MTR_4g111640 93.333 180 12 0 1 180 1 180 3.43e-126 353
MS.gene032997.t1 MTR_4g077787 85.475 179 24 2 1 178 1 178 7.92e-113 319
MS.gene032997.t1 MTR_4g112310 97.692 130 3 0 51 180 1 130 1.52e-93 268
MS.gene032997.t1 MTR_6g022170 55.833 120 50 2 23 140 25 143 2.59e-43 142
MS.gene032997.t1 MTR_2g101300 38.356 146 85 3 12 155 5 147 7.86e-33 116
MS.gene032997.t1 MTR_3g105930 40.506 158 83 6 31 180 26 180 1.30e-31 113
MS.gene032997.t1 MTR_4g078410 42.647 136 70 4 31 164 24 153 6.54e-30 108
MS.gene032997.t1 MTR_2g090580 39.161 143 73 5 31 164 26 163 8.92e-30 108
MS.gene032997.t1 MTR_2g079030 37.821 156 82 3 14 158 7 158 1.04e-27 108
MS.gene032997.t1 MTR_8g463180 41.007 139 73 3 11 147 6 137 3.50e-27 104
MS.gene032997.t1 MTR_8g086360 35.811 148 85 4 8 153 3 142 2.08e-25 97.4
MS.gene032997.t1 MTR_8g095013 37.190 121 71 2 13 131 6 123 4.45e-25 99.0
MS.gene032997.t1 MTR_1g014120 36.424 151 76 6 7 146 4 145 5.27e-25 96.3
MS.gene032997.t1 MTR_8g094990 36.364 121 72 2 13 131 6 123 2.22e-23 94.4
MS.gene032997.t1 MTR_2g090575 37.398 123 68 3 12 132 8 123 6.87e-23 94.4
MS.gene032997.t1 MTR_7g090170 37.226 137 64 6 29 160 24 143 8.46e-21 84.3
MS.gene032997.t1 MTR_4g124280 36.735 147 68 5 32 157 25 167 1.43e-20 85.9
MS.gene032997.t1 MTR_1g077790 37.143 105 63 2 29 131 26 129 1.94e-20 84.7
MS.gene032997.t1 MTR_8g095020 39.806 103 55 3 32 132 25 122 2.65e-20 86.7
MS.gene032997.t1 MTR_4g081100 34.426 122 72 3 13 132 17 132 2.96e-20 84.7
MS.gene032997.t1 MTR_2g083250 38.095 105 61 3 31 132 25 128 2.32e-19 83.2
MS.gene032997.t1 MTR_5g006040 31.138 167 101 6 26 183 20 181 1.20e-18 79.7
MS.gene032997.t1 MTR_4g114870 37.255 102 60 2 32 131 191 290 2.85e-18 81.6
MS.gene032997.t1 MTR_4g114870 31.667 120 77 3 30 146 27 144 4.91e-15 72.4
MS.gene032997.t1 MTR_0334s0010 35.294 102 62 2 31 131 27 125 5.92e-17 75.5
MS.gene032997.t1 MTR_4g067200 30.709 127 82 3 14 136 10 134 9.57e-17 74.3
MS.gene032997.t1 MTR_4g066110 30.709 127 82 3 14 136 10 134 9.57e-17 74.3
MS.gene032997.t1 MTR_1g090420 31.250 144 93 3 11 151 7 147 1.78e-16 74.7
MS.gene032997.t1 MTR_6g083240 32.215 149 88 5 31 171 26 169 7.16e-16 72.0
MS.gene032997.t1 MTR_1g104800 34.906 106 63 2 32 135 25 126 9.28e-16 72.0
MS.gene032997.t1 MTR_2g088990 32.174 115 70 3 29 141 22 130 1.10e-14 70.1
MS.gene032997.t1 MTR_3g092170 28.834 163 88 3 13 151 6 164 2.22e-14 69.7
MS.gene032997.t1 MTR_3g099570 31.690 142 84 6 14 151 7 139 3.93e-14 67.8
MS.gene032997.t1 MTR_6g023760 36.538 104 58 3 30 131 24 121 1.23e-13 66.2
MS.gene032997.t1 MTR_3g099580 34.127 126 71 4 9 131 4 120 1.30e-13 66.2
MS.gene032997.t1 MTR_6g013420 28.859 149 93 5 4 146 8 149 2.39e-13 65.5
MS.gene032997.t1 MTR_4g130780 33.588 131 76 6 7 131 4 129 6.45e-12 61.6
MS.gene032997.t1 MTR_3g099540 32.039 103 63 3 30 131 25 121 8.80e-12 60.1
MS.gene032997.t1 MTR_2g025580 27.811 169 110 5 14 176 7 169 1.04e-11 60.8
MS.gene032997.t1 MTR_4g114830 30.952 126 80 5 26 146 22 145 1.57e-11 60.1
MS.gene032997.t1 MTR_3g099980 32.710 107 63 3 30 133 24 124 7.85e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032997.t1 AT1G48940 48.851 174 71 4 11 173 10 176 3.35e-52 165
MS.gene032997.t1 AT3G18590 64.151 106 38 0 27 132 22 127 9.63e-50 159
MS.gene032997.t1 AT5G14345 43.704 135 70 2 12 146 1 129 8.41e-36 122
MS.gene032997.t1 AT1G79800 41.129 124 72 1 12 135 14 136 5.02e-31 112
MS.gene032997.t1 AT4G28365 38.194 144 78 3 12 148 9 148 1.73e-30 110
MS.gene032997.t1 AT4G31840 40.559 143 77 4 12 151 10 147 8.46e-30 108
MS.gene032997.t1 AT5G53870 35.632 174 92 5 1 164 1 164 7.82e-28 107
MS.gene032997.t1 AT5G53870 35.632 174 92 5 1 164 1 164 1.09e-27 107
MS.gene032997.t1 AT4G32490 36.429 140 83 2 28 165 27 162 2.16e-26 100
MS.gene032997.t1 AT2G25060 35.849 159 89 5 31 184 30 180 1.38e-25 97.8
MS.gene032997.t1 AT5G57920 40.945 127 65 5 25 143 13 137 4.25e-23 91.3
MS.gene032997.t1 AT4G30590 38.710 124 71 3 12 132 8 129 7.45e-23 90.9
MS.gene032997.t1 AT5G57920 43.810 105 54 3 31 132 22 124 1.48e-22 89.7
MS.gene032997.t1 AT5G57920 41.176 119 62 4 31 143 54 170 1.93e-22 90.5
MS.gene032997.t1 AT5G25090 41.667 120 64 4 17 133 12 128 7.92e-22 88.2
MS.gene032997.t1 AT4G27520 31.944 144 92 2 5 146 4 143 1.28e-20 87.8
MS.gene032997.t1 AT3G20570 36.538 104 61 2 31 132 29 129 3.92e-20 84.0
MS.gene032997.t1 AT1G64640 30.216 139 92 2 12 148 13 148 4.55e-20 83.6
MS.gene032997.t1 AT2G26720 36.585 123 71 3 12 132 9 126 7.18e-19 80.9
MS.gene032997.t1 AT2G31050 33.571 140 84 3 3 140 2 134 7.95e-19 80.5
MS.gene032997.t1 AT2G23990 34.483 145 81 7 12 151 11 146 1.49e-18 80.1
MS.gene032997.t1 AT2G32300 33.071 127 75 4 29 151 23 143 7.92e-18 79.0
MS.gene032997.t1 AT2G32300 33.333 126 74 4 30 151 54 173 1.32e-17 79.0
MS.gene032997.t1 AT3G60270 33.333 102 61 3 32 132 26 121 2.13e-14 68.6
MS.gene032997.t1 AT5G26330 29.851 134 80 4 1 132 1 122 2.38e-14 68.6
MS.gene032997.t1 AT2G23990 30.488 164 81 8 12 151 11 165 3.01e-14 68.9
MS.gene032997.t1 AT5G07475 30.657 137 87 3 12 146 10 140 1.07e-13 66.6
MS.gene032997.t1 AT2G27035 30.631 111 66 5 35 142 32 134 1.57e-11 60.5
MS.gene032997.t1 AT3G27200 33.663 101 59 3 34 132 28 122 8.49e-11 58.5

Find 40 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAAGTTGGTGGTAAAAT+TGG 0.248593 8.4:+17316292 MS.gene032997:CDS
TTTCTTTGCCTCCCAATCTT+TGG 0.264284 8.4:+17316252 MS.gene032997:CDS
TCATCACCATCCTTTGAGTT+AGG 0.279895 8.4:-17316325 None:intergenic
CTCTTTGCTTTGTCATTCCT+TGG 0.289880 8.4:+17317127 MS.gene032997:CDS
GGTGAGGGTGCTGTTGGTTC+AGG 0.348949 8.4:-17317025 None:intergenic
TACTTCATTAGTGGTGTTAG+TGG 0.359912 8.4:+17316953 MS.gene032997:CDS
GTGAATGCTGTGATAGTAAT+AGG 0.385754 8.4:-17317103 None:intergenic
TACAATTGATGACCAAAGAT+TGG 0.408666 8.4:-17316264 None:intergenic
CATCACCATCCTTTGAGTTA+GGG 0.425498 8.4:-17316324 None:intergenic
GGATTGTTCTACTTCATTAG+TGG 0.430222 8.4:+17316944 MS.gene032997:CDS
GTGATGAAATGTACAACAAA+TGG 0.453312 8.4:+17316341 MS.gene032997:CDS
GCAGCTTTAGAATGTGCTAT+TGG 0.459227 8.4:-17317070 None:intergenic
TCGACTCGTCCACTCTTCTT+CGG 0.480946 8.4:+17316884 MS.gene032997:CDS
GCAAAGGAAAAGTGGGTATG+AGG 0.483957 8.4:-17316221 None:intergenic
AGTTGGTGGTAAAATTGGTT+GGG 0.488516 8.4:+17316297 MS.gene032997:CDS
GCATTCTCATTTGCAGATTG+TGG 0.492826 8.4:-17317046 None:intergenic
ACAATTGATGACCAAAGATT+GGG 0.505414 8.4:-17316263 None:intergenic
GATTGTGGTGAGGGTGCTGT+TGG 0.512152 8.4:-17317031 None:intergenic
AATTGTACTGAATTTGAAGT+TGG 0.513642 8.4:+17316280 MS.gene032997:CDS
TCATTTGCAGATTGTGGTGA+GGG 0.517209 8.4:-17317040 None:intergenic
CCATTGTTTCCGAAGAAGAG+TGG 0.518696 8.4:-17316893 None:intergenic
CTCATTTGCAGATTGTGGTG+AGG 0.521847 8.4:-17317041 None:intergenic
ATAATCAAGCAAAGGAAAAG+TGG 0.531969 8.4:-17316229 None:intergenic
TGTACTGAATTTGAAGTTGG+TGG 0.533474 8.4:+17316283 MS.gene032997:CDS
CTAATGAAGTAGAACAATCC+TGG 0.545709 8.4:-17316941 None:intergenic
AAGTTGGTGGTAAAATTGGT+TGG 0.549766 8.4:+17316296 MS.gene032997:CDS
TGATGAAATGTACAACAAAT+GGG 0.552171 8.4:+17316342 MS.gene032997:CDS
TAATCAAGCAAAGGAAAAGT+GGG 0.571116 8.4:-17316228 None:intergenic
CCACTCTTCTTCGGAAACAA+TGG 0.572032 8.4:+17316893 MS.gene032997:CDS
CGAGAAAGATTCAGTGATGG+TGG 0.591369 8.4:+17316832 MS.gene032997:CDS
CTTCGGAAACAATGGCAACA+CGG 0.597439 8.4:+17316901 MS.gene032997:CDS
TGGTGGTAAAATTGGTTGGG+TGG 0.599284 8.4:+17316300 MS.gene032997:CDS
GTTAGTGGTCATTGTACAAG+AGG 0.601760 8.4:+17316968 MS.gene032997:CDS
CAAAGAAAATAATCAAGCAA+AGG 0.602288 8.4:-17316237 None:intergenic
CAAGCATAAATATCATGCCA+AGG 0.602952 8.4:-17317144 None:intergenic
TTGGGTGGTCCCTAACTCAA+AGG 0.612930 8.4:+17316315 MS.gene032997:CDS
GTATGAGGAAGCTTTGAAGA+TGG 0.616797 8.4:-17316206 None:intergenic
GTGGTCCCTAACTCAAAGGA+TGG 0.629713 8.4:+17316319 MS.gene032997:CDS
ATTGATGACCAAAGATTGGG+AGG 0.657515 8.4:-17316260 None:intergenic
GTACGAGAAAGATTCAGTGA+TGG 0.658831 8.4:+17316829 MS.gene032997:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACACACATATTTAAAAA+TGG - chr8.4:17316591-17316610 None:intergenic 15.0%
!! ACAAAAGTTATATAACAAAA+AGG - chr8.4:17316785-17316804 None:intergenic 15.0%
!! TTTAAAAATGGTAACATAAA+TGG - chr8.4:17316579-17316598 None:intergenic 15.0%
!!! ATCACAAATTTTTTAGAAAA+TGG - chr8.4:17316656-17316675 None:intergenic 15.0%
!!! CTTAAAATTAAATCTGTTTT+GGG - chr8.4:17316420-17316439 None:intergenic 15.0%
!!! TCTTAAAATTAAATCTGTTT+TGG - chr8.4:17316421-17316440 None:intergenic 15.0%
!!! TTTCTAAAAAGAAATGAATA+AGG + chr8.4:17316481-17316500 MS.gene032997:intron 15.0%
!!! ATCAATTTTGAATCTGTTTT+GGG - chr8.4:17316371-17316390 None:intergenic 20.0%
!!! TAACTTTTGTTTTGAACTTT+TGG + chr8.4:17316795-17316814 MS.gene032997:intron 20.0%
! AATAAATGTAATCTTTGTCG+TGG + chr8.4:17316758-17316777 MS.gene032997:intron 25.0%
! CAAAGAAAATAATCAAGCAA+AGG - chr8.4:17316240-17316259 None:intergenic 25.0%
! TGATGAAATGTACAACAAAT+GGG + chr8.4:17316342-17316361 MS.gene032997:CDS 25.0%
!! AAAAATGATCATCAAGGTTT+TGG + chr8.4:17316994-17317013 MS.gene032997:CDS 25.0%
!! AATTGTACTGAATTTGAAGT+TGG + chr8.4:17316280-17316299 MS.gene032997:CDS 25.0%
!! ATATCAATTACCAAAGCATT+GGG - chr8.4:17316619-17316638 None:intergenic 25.0%
!! CATCAATTTTGAATCTGTTT+TGG - chr8.4:17316372-17316391 None:intergenic 25.0%
AGGACAAAAAATGATCATCA+AGG + chr8.4:17316988-17317007 MS.gene032997:CDS 30.0%
ATAATCAAGCAAAGGAAAAG+TGG - chr8.4:17316232-17316251 None:intergenic 30.0%
GATATCAATTACCAAAGCAT+TGG - chr8.4:17316620-17316639 None:intergenic 30.0%
GTGATGAAATGTACAACAAA+TGG + chr8.4:17316341-17316360 MS.gene032997:CDS 30.0%
TAATCAAGCAAAGGAAAAGT+GGG - chr8.4:17316231-17316250 None:intergenic 30.0%
! ACAATTGATGACCAAAGATT+GGG - chr8.4:17316266-17316285 None:intergenic 30.0%
! GTTTTTATATCCCAATGCTT+TGG + chr8.4:17316606-17316625 MS.gene032997:intron 30.0%
! TACAATTGATGACCAAAGAT+TGG - chr8.4:17316267-17316286 None:intergenic 30.0%
!! CATTTATACCTTCACTTTTG+TGG - chr8.4:17316510-17316529 None:intergenic 30.0%
!! TTTGAAGTTGGTGGTAAAAT+TGG + chr8.4:17316292-17316311 MS.gene032997:CDS 30.0%
CAAGCATAAATATCATGCCA+AGG - chr8.4:17317147-17317166 None:intergenic 35.0%
CTAATGAAGTAGAACAATCC+TGG - chr8.4:17316944-17316963 None:intergenic 35.0%
GGATTGTTCTACTTCATTAG+TGG + chr8.4:17316944-17316963 MS.gene032997:CDS 35.0%
GTGAATGCTGTGATAGTAAT+AGG - chr8.4:17317106-17317125 None:intergenic 35.0%
! GTTTTCAAATTTGAACGACC+AGG + chr8.4:17316923-17316942 MS.gene032997:CDS 35.0%
! TACTTCATTAGTGGTGTTAG+TGG + chr8.4:17316953-17316972 MS.gene032997:CDS 35.0%
! TGTACTGAATTTGAAGTTGG+TGG + chr8.4:17316283-17316302 MS.gene032997:CDS 35.0%
!! AAGTTGGTGGTAAAATTGGT+TGG + chr8.4:17316296-17316315 MS.gene032997:CDS 35.0%
!! AGTTGGTGGTAAAATTGGTT+GGG + chr8.4:17316297-17316316 MS.gene032997:CDS 35.0%
!!! AAATCTGTTTTGGGAAGAAG+TGG - chr8.4:17316411-17316430 None:intergenic 35.0%
CTCTTTGCTTTGTCATTCCT+TGG + chr8.4:17317127-17317146 MS.gene032997:CDS 40.0%
GCATTCTCATTTGCAGATTG+TGG - chr8.4:17317049-17317068 None:intergenic 40.0%
GTACGAGAAAGATTCAGTGA+TGG + chr8.4:17316829-17316848 MS.gene032997:CDS 40.0%
GTTAGTGGTCATTGTACAAG+AGG + chr8.4:17316968-17316987 MS.gene032997:CDS 40.0%
TAAGGTCACCACAAAAGTGA+AGG + chr8.4:17316499-17316518 MS.gene032997:intron 40.0%
TCATTTGCAGATTGTGGTGA+GGG - chr8.4:17317043-17317062 None:intergenic 40.0%
TTTCTTTGCCTCCCAATCTT+TGG + chr8.4:17316252-17316271 MS.gene032997:CDS 40.0%
! ATTGATGACCAAAGATTGGG+AGG - chr8.4:17316263-17316282 None:intergenic 40.0%
! CATCACCATCCTTTGAGTTA+GGG - chr8.4:17316327-17316346 None:intergenic 40.0%
! TCATCACCATCCTTTGAGTT+AGG - chr8.4:17316328-17316347 None:intergenic 40.0%
!! GCAGCTTTAGAATGTGCTAT+TGG - chr8.4:17317073-17317092 None:intergenic 40.0%
!! GTATGAGGAAGCTTTGAAGA+TGG - chr8.4:17316209-17316228 None:intergenic 40.0%
CCACTCTTCTTCGGAAACAA+TGG + chr8.4:17316893-17316912 MS.gene032997:CDS 45.0%
CCATTGTTTCCGAAGAAGAG+TGG - chr8.4:17316896-17316915 None:intergenic 45.0%
CGAGAAAGATTCAGTGATGG+TGG + chr8.4:17316832-17316851 MS.gene032997:CDS 45.0%
CTCATTTGCAGATTGTGGTG+AGG - chr8.4:17317044-17317063 None:intergenic 45.0%
CTTCGGAAACAATGGCAACA+CGG + chr8.4:17316901-17316920 MS.gene032997:CDS 45.0%
GCAAAGGAAAAGTGGGTATG+AGG - chr8.4:17316224-17316243 None:intergenic 45.0%
TGGTGGTAAAATTGGTTGGG+TGG + chr8.4:17316300-17316319 MS.gene032997:CDS 45.0%
!!! ATGATATATTTTATTTTTAA+AGG + chr8.4:17316531-17316550 MS.gene032997:intron 5.0%
!!! TGATATATTTTATTTTTAAA+GGG + chr8.4:17316532-17316551 MS.gene032997:intron 5.0%
GTGGTCCCTAACTCAAAGGA+TGG + chr8.4:17316319-17316338 MS.gene032997:CDS 50.0%
TTGGGTGGTCCCTAACTCAA+AGG + chr8.4:17316315-17316334 MS.gene032997:CDS 50.0%
! TCGACTCGTCCACTCTTCTT+CGG + chr8.4:17316884-17316903 MS.gene032997:CDS 50.0%
!! GATTGTGGTGAGGGTGCTGT+TGG - chr8.4:17317034-17317053 None:intergenic 55.0%
!! GGTGAGGGTGCTGTTGGTTC+AGG - chr8.4:17317028-17317047 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 17316199 17317195 17316199 ID=MS.gene032997
chr8.4 mRNA 17316199 17317195 17316199 ID=MS.gene032997.t1;Parent=MS.gene032997
chr8.4 exon 17316199 17316400 17316199 ID=MS.gene032997.t1.exon1;Parent=MS.gene032997.t1
chr8.4 CDS 17316199 17316400 17316199 ID=cds.MS.gene032997.t1;Parent=MS.gene032997.t1
chr8.4 exon 17316822 17317195 17316822 ID=MS.gene032997.t1.exon2;Parent=MS.gene032997.t1
chr8.4 CDS 17316822 17317195 17316822 ID=cds.MS.gene032997.t1;Parent=MS.gene032997.t1
Gene Sequence

>MS.gene032997

ATGGCTGCCATCTTCAAAGCTTCCTCATACCCACTTTTCCTTTGCTTGATTATTTTCTTTGCCTCCCAATCTTTGGTCATCAATTGTACTGAATTTGAAGTTGGTGGTAAAATTGGTTGGGTGGTCCCTAACTCAAAGGATGGTGATGAAATGTACAACAAATGGGCTTCCCAAAACAGATTCAAAATTGATGATATTATTCGTAAGTACCACTTCTTCCCAAAACAGATTTAATTTTAAGATACATATATATATGTTATGTTTGAGTTGAGATCATCTTCATTTCTAAAAAGAAATGAATAAGGTCACCACAAAAGTGAAGGTATAAATGCATGATATATTTTATTTTTAAAGGGAAATGCATGATATATATGTCACCATTTATGTTACCATTTTTAAATATGTGTGTTTTTATATCCCAATGCTTTGGTAATTGATATCTTTATTGTTCGATCCATTTTCTAAAAAATTTGTGATCACAATTCTCGACAATTTTTCATACTATAGAGAATTACAATCACAAAAGACATAAATTTGATGTGTATAGATTGCAAAAAAAAATAAATGTAATCTTTGTCGTGGACCTTTTTGTTATATAACTTTTGTTTTGAACTTTTGGTTAGATTTCAAGTACGAGAAAGATTCAGTGATGGTGGTTAGTGAAGAAGAATATGAGAATTGCAAATCGACTCGTCCACTCTTCTTCGGAAACAATGGCAACACGGTTTTCAAATTTGAACGACCAGGATTGTTCTACTTCATTAGTGGTGTTAGTGGTCATTGTACAAGAGGACAAAAAATGATCATCAAGGTTTTGGATGTTGAACCTGAACCAACAGCACCCTCACCACAATCTGCAAATGAGAATGCACCAATAGCACATTCTAAAGCTGCTCAAATAACTCCTATTACTATCACAGCATTCACACTCTTTGCTTTGTCATTCCTTGGCATGATATTTATGCTTGAAGCACTAATTCGTGATCTTTTAATTTAG

Protein sequence

>MS.gene032997.t1

MAAIFKASSYPLFLCLIIFFASQSLVINCTEFEVGGKIGWVVPNSKDGDEMYNKWASQNRFKIDDIIHFKYEKDSVMVVSEEEYENCKSTRPLFFGNNGNTVFKFERPGLFYFISGVSGHCTRGQKMIIKVLDVEPEPTAPSPQSANENAPIAHSKAAQITPITITAFTLFALSFLGMIFMLEALIRDLLI