AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006553


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006553.t1 MTR_4g077787 92.135 178 14 0 1 178 1 178 2.23e-123 345
MS.gene006553.t1 MTR_4g111640 84.615 182 26 2 1 181 1 181 1.40e-114 323
MS.gene006553.t1 MTR_4g112310 88.636 132 13 2 51 181 1 131 4.99e-83 241
MS.gene006553.t1 MTR_6g022170 59.091 110 44 1 23 132 25 133 1.22e-44 145
MS.gene006553.t1 MTR_2g101300 37.584 149 86 3 15 157 8 155 2.13e-33 117
MS.gene006553.t1 MTR_4g078410 42.636 129 68 2 29 155 22 146 2.20e-30 109
MS.gene006553.t1 MTR_3g105930 39.130 161 83 6 29 179 24 179 3.46e-30 108
MS.gene006553.t1 MTR_2g090580 37.162 148 79 4 13 150 8 151 1.93e-29 107
MS.gene006553.t1 MTR_1g014120 39.189 148 75 6 7 145 4 145 1.66e-28 105
MS.gene006553.t1 MTR_8g463180 39.286 140 71 5 12 145 5 136 3.99e-27 104
MS.gene006553.t1 MTR_2g079030 38.194 144 83 2 14 155 7 146 1.60e-25 102
MS.gene006553.t1 MTR_8g086360 34.266 143 85 3 12 152 7 142 2.29e-25 97.1
MS.gene006553.t1 MTR_8g095013 36.885 122 69 3 12 131 8 123 1.57e-23 94.7
MS.gene006553.t1 MTR_4g081100 39.423 104 59 2 31 132 31 132 5.01e-22 89.0
MS.gene006553.t1 MTR_2g090575 35.714 126 72 3 12 132 2 123 5.15e-22 91.3
MS.gene006553.t1 MTR_8g094990 36.066 122 70 3 12 131 8 123 9.49e-22 89.4
MS.gene006553.t1 MTR_4g124280 34.161 161 78 4 32 170 25 179 9.89e-22 88.6
MS.gene006553.t1 MTR_1g077790 35.652 115 71 2 29 141 26 139 1.20e-21 87.4
MS.gene006553.t1 MTR_7g090170 37.778 135 62 6 29 158 24 141 2.92e-21 85.1
MS.gene006553.t1 MTR_8g095020 40.777 103 54 3 32 132 25 122 1.30e-20 87.0
MS.gene006553.t1 MTR_2g083250 39.048 105 60 3 31 132 25 128 6.45e-20 84.3
MS.gene006553.t1 MTR_5g006040 31.944 144 90 3 14 153 8 147 1.63e-19 81.6
MS.gene006553.t1 MTR_1g090420 29.714 175 99 4 16 169 8 179 2.48e-18 79.3
MS.gene006553.t1 MTR_3g099570 31.507 146 90 4 7 150 2 139 1.75e-17 76.3
MS.gene006553.t1 MTR_4g114870 36.275 102 61 2 32 131 191 290 2.60e-17 78.6
MS.gene006553.t1 MTR_4g114870 30.714 140 88 4 13 145 7 144 4.72e-16 75.1
MS.gene006553.t1 MTR_1g104800 34.906 106 63 2 32 135 25 126 1.08e-16 74.3
MS.gene006553.t1 MTR_4g130780 32.374 139 84 5 8 140 4 138 3.47e-15 70.1
MS.gene006553.t1 MTR_3g099580 34.783 115 62 3 24 131 12 120 3.98e-15 70.1
MS.gene006553.t1 MTR_3g092170 32.540 126 70 4 12 129 2 120 4.52e-15 71.6
MS.gene006553.t1 MTR_4g067200 29.688 128 84 3 13 136 9 134 4.88e-15 69.7
MS.gene006553.t1 MTR_4g066110 29.688 128 84 3 13 136 9 134 4.88e-15 69.7
MS.gene006553.t1 MTR_6g023760 37.500 104 57 3 30 131 24 121 5.42e-15 69.7
MS.gene006553.t1 MTR_0334s0010 30.833 120 79 2 13 131 9 125 5.97e-15 70.1
MS.gene006553.t1 MTR_6g083240 30.519 154 95 5 14 157 8 159 1.11e-14 68.6
MS.gene006553.t1 MTR_2g088990 33.028 109 65 3 29 135 22 124 2.92e-14 68.6
MS.gene006553.t1 MTR_3g099540 29.688 128 70 4 11 131 7 121 1.08e-13 65.1
MS.gene006553.t1 MTR_3g099980 33.333 126 74 4 11 133 6 124 2.12e-13 66.2
MS.gene006553.t1 MTR_3g099680 33.333 126 74 4 11 133 6 124 2.16e-13 66.2
MS.gene006553.t1 MTR_6g013420 30.137 146 88 5 7 145 10 148 2.30e-13 65.1
MS.gene006553.t1 MTR_8g099220 28.000 125 84 3 10 131 6 127 6.22e-13 62.8
MS.gene006553.t1 MTR_8g007020 29.730 148 80 6 29 158 21 162 7.30e-12 61.2
MS.gene006553.t1 MTR_7g086100 32.031 128 78 3 7 132 2 122 1.73e-11 61.2
MS.gene006553.t1 MTR_2g025580 26.846 149 96 5 34 175 29 171 3.01e-11 59.3
MS.gene006553.t1 MTR_1g090190 28.906 128 80 3 25 147 16 137 3.19e-11 59.7
MS.gene006553.t1 MTR_4g130800 37.209 86 51 2 54 137 50 134 3.47e-11 60.5
MS.gene006553.t1 MTR_4g130800 37.209 86 51 2 54 137 49 133 4.19e-11 60.5
MS.gene006553.t1 MTR_8g007035 33.981 103 61 2 30 131 22 118 5.14e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006553.t1 AT1G48940 47.093 172 77 3 11 173 10 176 3.01e-56 175
MS.gene006553.t1 AT3G18590 58.400 125 52 0 8 132 3 127 6.99e-54 169
MS.gene006553.t1 AT5G14345 44.262 122 66 2 25 145 9 129 7.17e-34 117
MS.gene006553.t1 AT1G79800 40.800 125 73 1 11 135 13 136 3.85e-32 114
MS.gene006553.t1 AT4G28365 35.948 153 92 2 12 162 9 157 1.95e-30 110
MS.gene006553.t1 AT4G31840 37.736 159 92 5 7 160 2 158 6.26e-29 105
MS.gene006553.t1 AT4G32490 44.860 107 53 2 28 132 27 129 4.84e-27 102
MS.gene006553.t1 AT4G30590 41.129 124 68 3 12 132 8 129 1.56e-26 100
MS.gene006553.t1 AT5G53870 35.227 176 96 6 1 167 1 167 2.38e-26 103
MS.gene006553.t1 AT5G53870 35.227 176 96 6 1 167 1 167 2.77e-26 103
MS.gene006553.t1 AT5G57920 39.552 134 70 5 18 142 6 137 1.80e-25 97.1
MS.gene006553.t1 AT2G25060 40.152 132 72 5 24 150 23 152 1.97e-25 97.1
MS.gene006553.t1 AT5G57920 41.176 119 61 4 31 142 22 138 8.09e-25 95.5
MS.gene006553.t1 AT5G57920 41.176 119 61 4 31 142 54 170 1.05e-24 95.9
MS.gene006553.t1 AT4G27520 32.298 161 97 4 5 162 4 155 2.48e-22 92.4
MS.gene006553.t1 AT3G20570 33.333 138 75 4 13 148 15 137 2.12e-21 87.0
MS.gene006553.t1 AT5G25090 39.837 123 62 6 16 132 11 127 7.66e-21 85.1
MS.gene006553.t1 AT2G31050 36.029 136 74 4 11 142 12 138 1.06e-20 85.1
MS.gene006553.t1 AT2G23990 36.296 135 75 5 1 131 1 128 5.36e-20 83.6
MS.gene006553.t1 AT1G64640 33.333 108 65 3 27 131 28 131 1.86e-19 81.6
MS.gene006553.t1 AT2G32300 30.405 148 92 4 8 150 2 143 2.55e-19 82.8
MS.gene006553.t1 AT2G32300 30.405 148 92 4 8 150 32 173 4.75e-19 82.4
MS.gene006553.t1 AT2G26720 34.559 136 76 4 11 142 12 138 7.95e-19 80.5
MS.gene006553.t1 AT5G26330 35.922 103 60 2 32 132 24 122 6.28e-16 72.4
MS.gene006553.t1 AT2G23990 32.468 154 74 6 1 131 1 147 7.94e-16 72.8
MS.gene006553.t1 AT3G60270 33.333 102 61 2 32 132 26 121 1.79e-15 71.2
MS.gene006553.t1 AT5G07475 30.667 150 92 5 14 158 13 155 2.10e-14 68.6
MS.gene006553.t1 AT1G45063 32.456 114 66 3 25 132 19 127 2.54e-12 63.9
MS.gene006553.t1 AT1G45063 33.981 103 61 3 32 131 149 247 2.94e-12 63.5
MS.gene006553.t1 AT1G72230 30.693 101 69 1 52 151 37 137 3.08e-12 62.4
MS.gene006553.t1 AT1G45063 32.143 112 68 2 24 132 21 127 4.50e-12 63.9
MS.gene006553.t1 AT1G45063 33.981 103 61 3 32 131 149 247 5.72e-12 63.5
MS.gene006553.t1 AT3G27200 33.663 101 59 3 34 132 28 122 2.31e-11 59.7
MS.gene006553.t1 AT3G17675 30.645 124 77 3 11 131 10 127 2.55e-11 58.9

Find 27 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATGATCATCAAGGTTT+TGG 0.254697 4.4:-32862616 MS.gene006553:CDS
TCACAAAATAGATTCAAAAT+TGG 0.280437 4.4:-32863181 MS.gene006553:CDS
TGTGGTGCTGGTGCTGTAAT+TGG 0.293215 4.4:+32862585 None:intergenic
CTCTTTACTTTGTCATTCCT+TGG 0.308362 4.4:-32862483 MS.gene006553:CDS
TACTTCATCAGTGGAGTTAG+TGG 0.390509 4.4:-32862657 MS.gene006553:CDS
GTGCATGATGTGATAGTAAT+AGG 0.401733 4.4:+32862507 None:intergenic
TCATCTTTGTCCTTTGAGTC+AGG 0.404812 4.4:+32863223 None:intergenic
TCATTTGCAGATTGTGGTGC+TGG 0.425648 4.4:+32862573 None:intergenic
TTTGAAGTTGGTGGTAGAAT+TGG 0.429149 4.4:-32863256 MS.gene006553:CDS
CTGATGAAGTAGAACATTCC+TGG 0.429651 4.4:+32862669 None:intergenic
GCATTAGAATGTTGTGCTAT+TGG 0.440808 4.4:+32862543 None:intergenic
AGTTGGTGGTAGAATTGGTT+GGG 0.458216 4.4:-32863251 MS.gene006553:CDS
GCATTCTCATTTGCAGATTG+TGG 0.492826 4.4:+32862567 None:intergenic
AACTGTACTGAATTTGAAGT+TGG 0.536560 4.4:-32863268 MS.gene006553:CDS
TTGGGTTGTGCCTGACTCAA+AGG 0.571720 4.4:-32863233 MS.gene006553:CDS
AAGTTGGTGGTAGAATTGGT+TGG 0.575738 4.4:-32863252 MS.gene006553:CDS
AGGCCAGAAAATGATCATCA+AGG 0.582370 4.4:-32862622 MS.gene006553:CDS
GTGTGAGGCAGCTTTGAAGA+TGG 0.582821 4.4:+32863342 None:intergenic
TGTACTGAATTTGAAGTTGG+TGG 0.583292 4.4:-32863265 MS.gene006553:CDS
AAGATGATATGTACAACCGA+TGG 0.592618 4.4:-32863207 MS.gene006553:CDS
GTTAGTGGTCATTGTACAAG+AGG 0.592736 4.4:-32862642 MS.gene006553:CDS
TTCAAGCATAAATCATGCCA+AGG 0.596027 4.4:+32862466 None:intergenic
GCAAAGTAAAAGTGAGTGTG+AGG 0.606729 4.4:+32863327 None:intergenic
AGATGATATGTACAACCGAT+GGG 0.614363 4.4:-32863206 MS.gene006553:CDS
TGAGAAAGATTCAGTGATGG+TGG 0.639612 4.4:-32862778 MS.gene006553:CDS
GGAATGTTCTACTTCATCAG+TGG 0.644882 4.4:-32862666 MS.gene006553:CDS
GTATGAGAAAGATTCAGTGA+TGG 0.665783 4.4:-32862781 MS.gene006553:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTCATTTATAAAAATAAA+TGG + chr4.4:32862891-32862910 None:intergenic 10.0%
!!! TTTATTTTTATAAATGAAGA+TGG - chr4.4:32862891-32862910 MS.gene006553:intron 10.0%
!! AAAATTAAATTGGTAAGAAA+TGG + chr4.4:32862679-32862698 None:intergenic 15.0%
!! TATATATGGCAAAATTAAAT+TGG + chr4.4:32862689-32862708 None:intergenic 15.0%
!! ACATATTACTCCTAAAAAAA+TGG + chr4.4:32862814-32862833 None:intergenic 20.0%
!! TCACAAAATAGATTCAAAAT+TGG - chr4.4:32862635-32862654 MS.gene006553:CDS 20.0%
!!! ATATATGTCACCATTTTTTT+AGG - chr4.4:32862801-32862820 MS.gene006553:intron 20.0%
!!! ATATCCATTACTAAAGTTTT+GGG + chr4.4:32862854-32862873 None:intergenic 20.0%
!!! TATATCCATTACTAAAGTTT+TGG + chr4.4:32862855-32862874 None:intergenic 20.0%
! AATTCAGAACCTTTATATGT+CGG + chr4.4:32862980-32862999 None:intergenic 25.0%
! ATATCCCAAAACTTTAGTAA+TGG - chr4.4:32862847-32862866 MS.gene006553:intron 25.0%
! ATGTCTATTCTTAAAGTTGT+TGG - chr4.4:32862764-32862783 MS.gene006553:CDS 25.0%
! TGACATACATAGCATATATA+TGG + chr4.4:32862703-32862722 None:intergenic 25.0%
ATATGTCGGATTCTACATAT+TGG + chr4.4:32862966-32862985 None:intergenic 30.0%
ATGTAGAATCCGACATATAA+AGG - chr4.4:32862968-32862987 MS.gene006553:intron 30.0%
CCATTGTTGCCAAAAAATAA+TGG + chr4.4:32863102-32863121 None:intergenic 30.0%
TTGGTAAGAAATGGTACTTA+CGG + chr4.4:32862670-32862689 None:intergenic 30.0%
! AACTGTACTGAATTTGAAGT+TGG - chr4.4:32862548-32862567 MS.gene006553:CDS 30.0%
! GAAAATGATCATCAAGGTTT+TGG - chr4.4:32863200-32863219 MS.gene006553:CDS 30.0%
!! AAACCTTGATGATCATTTTC+TGG + chr4.4:32863200-32863219 None:intergenic 30.0%
!! ACAGTTGATGACCAAAAATT+GGG + chr4.4:32862534-32862553 None:intergenic 30.0%
!! TACAGTTGATGACCAAAAAT+TGG + chr4.4:32862535-32862554 None:intergenic 30.0%
!!! CCATTATTTTTTGGCAACAA+TGG - chr4.4:32863099-32863118 MS.gene006553:intron 30.0%
AAGATGATATGTACAACCGA+TGG - chr4.4:32862609-32862628 MS.gene006553:CDS 35.0%
AGATGATATGTACAACCGAT+GGG - chr4.4:32862610-32862629 MS.gene006553:CDS 35.0%
CTCTTTACTTTGTCATTCCT+TGG - chr4.4:32863333-32863352 MS.gene006553:CDS 35.0%
GCATTAGAATGTTGTGCTAT+TGG + chr4.4:32863276-32863295 None:intergenic 35.0%
GTATGAGAAAGATTCAGTGA+TGG - chr4.4:32863035-32863054 MS.gene006553:intron 35.0%
GTGCATGATGTGATAGTAAT+AGG + chr4.4:32863312-32863331 None:intergenic 35.0%
TAAGAAATGGTACTTACGGA+CGG + chr4.4:32862666-32862685 None:intergenic 35.0%
! ATCTATTTTGTGAAGCCCAT+CGG + chr4.4:32862628-32862647 None:intergenic 35.0%
! TGTACTGAATTTGAAGTTGG+TGG - chr4.4:32862551-32862570 MS.gene006553:CDS 35.0%
!! TTTGAAGTTGGTGGTAGAAT+TGG - chr4.4:32862560-32862579 MS.gene006553:CDS 35.0%
!!! TCCACTCGTCCATTATTTTT+TGG - chr4.4:32863090-32863109 MS.gene006553:intron 35.0%
AGGCCAGAAAATGATCATCA+AGG - chr4.4:32863194-32863213 MS.gene006553:CDS 40.0%
CTGATGAAGTAGAACATTCC+TGG + chr4.4:32863150-32863169 None:intergenic 40.0%
GCAAAGTAAAAGTGAGTGTG+AGG + chr4.4:32862492-32862511 None:intergenic 40.0%
GCATTCTCATTTGCAGATTG+TGG + chr4.4:32863252-32863271 None:intergenic 40.0%
GGAATGTTCTACTTCATCAG+TGG - chr4.4:32863150-32863169 MS.gene006553:intron 40.0%
GTTAGTGGTCATTGTACAAG+AGG - chr4.4:32863174-32863193 MS.gene006553:CDS 40.0%
TACTTCATCAGTGGAGTTAG+TGG - chr4.4:32863159-32863178 MS.gene006553:intron 40.0%
TCATCTTTGTCCTTTGAGTC+AGG + chr4.4:32862596-32862615 None:intergenic 40.0%
TGAGAAAGATTCAGTGATGG+TGG - chr4.4:32863038-32863057 MS.gene006553:intron 40.0%
! GTTTTCAAATTTGAGCGACC+AGG - chr4.4:32863129-32863148 MS.gene006553:intron 40.0%
! TTTCTCAGCCTCCCAATTTT+TGG - chr4.4:32862520-32862539 MS.gene006553:CDS 40.0%
!! AAGTTGGTGGTAGAATTGGT+TGG - chr4.4:32862564-32862583 MS.gene006553:CDS 40.0%
!! AGTTGGTGGTAGAATTGGTT+GGG - chr4.4:32862565-32862584 MS.gene006553:CDS 40.0%
!! GCCAAAAAATAATGGACGAG+TGG + chr4.4:32863094-32863113 None:intergenic 40.0%
!! GTTGATGACCAAAAATTGGG+AGG + chr4.4:32862531-32862550 None:intergenic 40.0%
TCATTTGCAGATTGTGGTGC+TGG + chr4.4:32863246-32863265 None:intergenic 45.0%
! TTGGGTTGTGCCTGACTCAA+AGG - chr4.4:32862583-32862602 MS.gene006553:CDS 50.0%
!! GTGTGAGGCAGCTTTGAAGA+TGG + chr4.4:32862477-32862496 None:intergenic 50.0%
!! TGTGGTGCTGGTGCTGTAAT+TGG + chr4.4:32863234-32863253 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 32862467 32863371 32862467 ID=MS.gene006553
chr4.4 mRNA 32862467 32863371 32862467 ID=MS.gene006553.t1;Parent=MS.gene006553
chr4.4 exon 32863170 32863371 32863170 ID=MS.gene006553.t1.exon1;Parent=MS.gene006553.t1
chr4.4 CDS 32863170 32863371 32863170 ID=cds.MS.gene006553.t1;Parent=MS.gene006553.t1
chr4.4 exon 32862467 32862810 32862467 ID=MS.gene006553.t1.exon2;Parent=MS.gene006553.t1
chr4.4 CDS 32862467 32862810 32862467 ID=cds.MS.gene006553.t1;Parent=MS.gene006553.t1
Gene Sequence

>MS.gene006553

ATGGCTTCCATCTTCAAAGCTGCCTCACACTCACTTTTACTTTGCTTCATTCTTTTCTCAGCCTCCCAATTTTTGGTCATCAACTGTACTGAATTTGAAGTTGGTGGTAGAATTGGTTGGGTTGTGCCTGACTCAAAGGACAAAGATGATATGTACAACCGATGGGCTTCACAAAATAGATTCAAAATTGGTGATACCGTCCGTAAGTACCATTTCTTACCAATTTAATTTTGCCATATATATGCTATGTATGTCATGTTTTTAGTATAAATCATCTATTTTTCAATGAAAATGAAGATGTCTATTCTTAAAGTTGTTGGAATACAAATGCATGATATATGTCACCATTTTTTTAGGAGTAATATGTGTGCATTTATATTATATCCCAAAACTTTAGTAATGGATATATTCACTTCTCAATCCATTTATTTTTATAAATGAAGATGGTCTCAACTCAACTTGTTATTATGTATAATACATCATACATGTACACATTCCAATATGTAGAATCCGACATATAAAGGTTCTGAATTACAATTTTAAATGTATCGCGATTTTTAGATTTCAAGTATGAGAAAGATTCAGTGATGGTGGTGAGTGAAGAAGAGTATGAACAATGCAAATCCACTCGTCCATTATTTTTTGGCAACAATGGCAACACAGTTTTCAAATTTGAGCGACCAGGAATGTTCTACTTCATCAGTGGAGTTAGTGGTCATTGTACAAGAGGCCAGAAAATGATCATCAAGGTTTTGGATGTCGAGCCAATTACAGCACCAGCACCACAATCTGCAAATGAGAATGCACCAATAGCACAACATTCTAATGCTGCTCAAATGACTCCTATTACTATCACATCATGCACACTCTTTACTTTGTCATTCCTTGGCATGATTTATGCTTGA

Protein sequence

>MS.gene006553.t1

MASIFKAASHSLLLCFILFSASQFLVINCTEFEVGGRIGWVVPDSKDKDDMYNRWASQNRFKIGDTVHFKYEKDSVMVVSEEEYEQCKSTRPLFFGNNGNTVFKFERPGMFYFISGVSGHCTRGQKMIIKVLDVEPITAPAPQSANENAPIAQHSNAAQMTPITITSCTLFTLSFLGMIYA