AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015235


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015235.t1 MTR_5g015880 96.104 231 9 0 1 231 1 231 3.03e-165 455
MS.gene015235.t1 MTR_4g095600 70.539 241 53 6 1 231 1 233 3.20e-112 321
MS.gene015235.t1 MTR_1g106420 56.579 152 61 3 1 147 1 152 7.66e-55 174
MS.gene015235.t1 MTR_7g007010 64.545 110 39 0 1 110 3 112 1.79e-52 171
MS.gene015235.t1 MTR_6g018270 61.818 110 42 0 1 110 3 112 5.99e-50 164
MS.gene015235.t1 MTR_6g453130 48.684 152 71 4 1 147 3 152 5.79e-46 152
MS.gene015235.t1 MTR_1g110840 51.128 133 61 2 1 131 3 133 5.38e-45 150
MS.gene015235.t1 MTR_6g054150 48.120 133 65 3 1 131 3 133 9.29e-41 139
MS.gene015235.t1 MTR_6g054080 47.368 133 66 3 1 131 3 133 1.30e-39 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015235.t1 AT4G37540 56.452 248 83 8 1 231 1 240 9.07e-83 247
MS.gene015235.t1 AT3G49940 57.031 256 76 10 1 231 1 247 9.26e-81 242
MS.gene015235.t1 AT5G67420 52.941 255 91 6 1 231 1 250 1.58e-75 229
MS.gene015235.t1 AT5G67420 52.941 255 91 6 1 231 1 250 1.58e-75 229
MS.gene015235.t1 AT1G67100 65.455 110 38 0 1 110 3 112 3.19e-52 169
MS.gene015235.t1 AT3G02550 63.636 110 40 0 1 110 3 112 7.61e-50 164
MS.gene015235.t1 AT1G68510 60.177 113 43 1 1 111 3 115 7.64e-47 155

Find 76 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCATGAACCGGACTTTC+TGG 0.160468 5.4:-9428843 None:intergenic
GAAACTCCGGTAAGGGTTTA+AGG 0.160922 5.4:-9428732 None:intergenic
ACGCGTTGACCAACGGTTTC+CGG 0.249914 5.4:+9428950 MS.gene015235:CDS
ATTAAAATTCTCAAACAAAA+AGG 0.262922 5.4:-9429098 None:intergenic
GTGGCATGACCTTGAGCTTC+AGG 0.279564 5.4:-9428357 None:intergenic
ACTCACTACAACCCTTTCTA+AGG 0.331551 5.4:-9428296 None:intergenic
GAATTCATCGACGGTGTTCC+CGG 0.351208 5.4:-9428988 None:intergenic
GGACTTTCTGGTTCGGATCT+CGG 0.355586 5.4:-9428831 None:intergenic
AGTCCGACAGCACCGTTAAC+GGG 0.370602 5.4:-9428646 None:intergenic
ACGCGTTCTGTGGCGTTAAC+CGG 0.381628 5.4:-9428933 None:intergenic
CGGAACCCTTAAACCCTTAC+CGG 0.384595 5.4:+9428726 MS.gene015235:CDS
AAAGTCCGGTTCATGAGTTC+TGG 0.390533 5.4:+9428847 MS.gene015235:CDS
TATTACGCCCTTGTATTCAA+TGG 0.394479 5.4:+9428325 MS.gene015235:CDS
AAGTCCGACAGCACCGTTAA+CGG 0.413291 5.4:-9428647 None:intergenic
AAACTCCGGTAAGGGTTTAA+GGG 0.432686 5.4:-9428731 None:intergenic
AAACCCTTACCGGAGTTTCT+CGG 0.442329 5.4:+9428736 MS.gene015235:CDS
CGGTTGCCGTGTCCTTAGAA+AGG 0.456637 5.4:+9428284 MS.gene015235:CDS
GGTGGTAAACGCAAGCGATC+AGG 0.465080 5.4:+9428868 MS.gene015235:CDS
TACCACCAGAACTCATGAAC+CGG 0.478203 5.4:-9428852 None:intergenic
ATGAACCGGACTTTCTGGTT+CGG 0.483553 5.4:-9428838 None:intergenic
AGCACCGTTAACGGGGTTCA+CGG 0.485215 5.4:-9428638 None:intergenic
TGTGACGACGGTGACGTGCT+TGG 0.492180 5.4:+9429020 MS.gene015235:CDS
TGGCATGACCTTGAGCTTCA+GGG 0.492713 5.4:-9428356 None:intergenic
CGGAGGGAAATTCGCCGGCC+GGG 0.499369 5.4:+9428970 MS.gene015235:CDS
AGAACCGTGAACCCCGTTAA+CGG 0.510533 5.4:+9428634 MS.gene015235:CDS
CCGGAGGGAAATTCGCCGGC+CGG 0.514516 5.4:+9428969 MS.gene015235:CDS
GTGTCAAGCGGCGGTTGAAA+CGG 0.518728 5.4:+9428693 MS.gene015235:CDS
TTACCAGGTCTCTGTGGTGA+AGG 0.520683 5.4:-9428438 None:intergenic
GGTTGCCGTGTCCTTAGAAA+GGG 0.520856 5.4:+9428285 MS.gene015235:CDS
CGTTAGAAATGAAAGACATA+AGG 0.526325 5.4:-9428413 None:intergenic
GCGTCTAAACCGAGAAACTC+CGG 0.534500 5.4:-9428745 None:intergenic
GAGATCCGAACCAGAAAGTC+CGG 0.539752 5.4:+9428833 MS.gene015235:CDS
TCATCGACGGTGTTCCCGGC+CGG 0.542141 5.4:-9428984 None:intergenic
ACGGTGACGTGCTTGGAAAG+TGG 0.543942 5.4:+9429027 MS.gene015235:CDS
GTATCGATCCATTGAATACA+AGG 0.548656 5.4:-9428333 None:intergenic
CGGTGACGTGCTTGGAAAGT+GGG 0.551329 5.4:+9429028 MS.gene015235:CDS
TACCAGGTCTCTGTGGTGAA+GGG 0.560196 5.4:-9428437 None:intergenic
TTCTGAGGAATCTGTGACGA+CGG 0.564218 5.4:+9429008 MS.gene015235:CDS
TGCTTGGAAAGTGGGATCGG+AGG 0.566383 5.4:+9429036 MS.gene015235:CDS
AAACCGAGAAACTCCGGTAA+GGG 0.570584 5.4:-9428739 None:intergenic
TAAACCGAGAAACTCCGGTA+AGG 0.571879 5.4:-9428740 None:intergenic
CCGTTGGTCAACGCGTTCTG+TGG 0.575430 5.4:-9428943 None:intergenic
GTTTCCGGAGGGAAATTCGC+CGG 0.577846 5.4:+9428965 MS.gene015235:CDS
AATTTCCCTCCGGAAACCGT+TGG 0.583258 5.4:-9428959 None:intergenic
ACCGTTGATGACTCATCTGA+AGG 0.585962 5.4:+9428778 MS.gene015235:CDS
ACGTGCTTGGAAAGTGGGAT+CGG 0.586938 5.4:+9429033 MS.gene015235:CDS
CCGGCCGGCGAATTTCCCTC+CGG 0.587093 5.4:-9428969 None:intergenic
AACCCCGTTAACGGTGCTGT+CGG 0.588780 5.4:+9428643 MS.gene015235:CDS
GTCCCTTCACCACAGAGACC+TGG 0.592710 5.4:+9428435 MS.gene015235:CDS
AGATTAAGATCAGTCGTCGC+CGG 0.593377 5.4:-9428910 None:intergenic
TCCTTCAGATGAGTCATCAA+CGG 0.596231 5.4:-9428779 None:intergenic
CGACGACTGATCTTAATCTC+CGG 0.598907 5.4:+9428914 MS.gene015235:CDS
AAGTCACGTGCAACGACGCG+CGG 0.599047 5.4:+9428803 MS.gene015235:CDS
CGTTGACCAACGGTTTCCGG+AGG 0.601993 5.4:+9428953 MS.gene015235:CDS
CCACAGAACGCGTTGACCAA+CGG 0.603462 5.4:+9428943 MS.gene015235:CDS
AGGGTTTAAGGGTTCCGCCA+CGG 0.607346 5.4:-9428720 None:intergenic
GGCATGACCTTGAGCTTCAG+GGG 0.607961 5.4:-9428355 None:intergenic
TACAACCCTTTCTAAGGACA+CGG 0.609661 5.4:-9428290 None:intergenic
ATCGATACCCCTGAAGCTCA+AGG 0.611816 5.4:+9428348 MS.gene015235:CDS
CAACTAGGTATGAGTTGCAA+CGG 0.621586 5.4:+9428264 None:intergenic
GTCCGGTTCATGAGTTCTGG+TGG 0.622596 5.4:+9428850 MS.gene015235:CDS
TGAAAGACATAAGGTCAGCA+CGG 0.634887 5.4:-9428404 None:intergenic
GTCCGACAGCACCGTTAACG+GGG 0.636214 5.4:-9428645 None:intergenic
GTTGAAACGGTGCTCCGTGG+CGG 0.644097 5.4:+9428706 MS.gene015235:CDS
AAACTGGCACGTGTGTCAAG+CGG 0.645348 5.4:+9428681 MS.gene015235:CDS
GTTGACCAACGGTTTCCGGA+GGG 0.646523 5.4:+9428954 MS.gene015235:CDS
CTGGCACGTGTGTCAAGCGG+CGG 0.654365 5.4:+9428684 MS.gene015235:CDS
TTCAGATGAGTCATCAACGG+TGG 0.656087 5.4:-9428776 None:intergenic
GCGGTTGAAACGGTGCTCCG+TGG 0.657002 5.4:+9428703 MS.gene015235:CDS
GATTATTTACCAGGTCTCTG+TGG 0.665083 5.4:-9428444 None:intergenic
TCTTTGTTGTTTGAAGCGTG+TGG 0.669278 5.4:+9428610 MS.gene015235:CDS
GATTCCTCAGAATTCATCGA+CGG 0.685600 5.4:-9428997 None:intergenic
TATCGATCCATTGAATACAA+GGG 0.699979 5.4:-9428332 None:intergenic
AACACCGTCGATGAATTCTG+AGG 0.704407 5.4:+9428993 MS.gene015235:CDS
AAACTTGGCTACAAAGACAG+TGG 0.714204 5.4:-9428376 None:intergenic
GGAGATAACTACGTTCACGA+CGG 0.740228 5.4:+9429057 MS.gene015235:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAAGATAAAGATTATTTACC+AGG - chr5.4:9428456-9428475 None:intergenic 25.0%
! TAGATGAACTAAACTAACTA+AGG + chr5.4:9428556-9428575 MS.gene015235:intron 25.0%
CGTTAGAAATGAAAGACATA+AGG - chr5.4:9428416-9428435 None:intergenic 30.0%
TATCGATCCATTGAATACAA+GGG - chr5.4:9428335-9428354 None:intergenic 30.0%
CAAAAAGGTTGAGAACTTTG+CGG - chr5.4:9429086-9429105 None:intergenic 35.0%
TATTACGCCCTTGTATTCAA+TGG + chr5.4:9428325-9428344 MS.gene015235:CDS 35.0%
! GTATCGATCCATTGAATACA+AGG - chr5.4:9428336-9428355 None:intergenic 35.0%
! GTCTTTGTAGCCAAGTTTTT+CGG + chr5.4:9428381-9428400 MS.gene015235:CDS 35.0%
! TTAGAGTGAAAGTGTAACTG+TGG - chr5.4:9428490-9428509 None:intergenic 35.0%
AAACTCCGGTAAGGGTTTAA+GGG - chr5.4:9428734-9428753 None:intergenic 40.0%
AAACTTGGCTACAAAGACAG+TGG - chr5.4:9428379-9428398 None:intergenic 40.0%
ACTCACTACAACCCTTTCTA+AGG - chr5.4:9428299-9428318 None:intergenic 40.0%
GATTATTTACCAGGTCTCTG+TGG - chr5.4:9428447-9428466 None:intergenic 40.0%
GATTCCTCAGAATTCATCGA+CGG - chr5.4:9429000-9429019 None:intergenic 40.0%
TCCTTCAGATGAGTCATCAA+CGG - chr5.4:9428782-9428801 None:intergenic 40.0%
TGAAAGACATAAGGTCAGCA+CGG - chr5.4:9428407-9428426 None:intergenic 40.0%
! TACAACCCTTTCTAAGGACA+CGG - chr5.4:9428293-9428312 None:intergenic 40.0%
!! TCTTTGTTGTTTGAAGCGTG+TGG + chr5.4:9428610-9428629 MS.gene015235:CDS 40.0%
!!! TGCTGAAGTTTTGAAGCTTC+CGG + chr5.4:9428891-9428910 MS.gene015235:CDS 40.0%
AAACCCTTACCGGAGTTTCT+CGG + chr5.4:9428736-9428755 MS.gene015235:CDS 45.0%
AAACCGAGAAACTCCGGTAA+GGG - chr5.4:9428742-9428761 None:intergenic 45.0%
AAAGTCCGGTTCATGAGTTC+TGG + chr5.4:9428847-9428866 MS.gene015235:CDS 45.0%
AACACCGTCGATGAATTCTG+AGG + chr5.4:9428993-9429012 MS.gene015235:CDS 45.0%
AACTCATGAACCGGACTTTC+TGG - chr5.4:9428846-9428865 None:intergenic 45.0%
AGATTAAGATCAGTCGTCGC+CGG - chr5.4:9428913-9428932 None:intergenic 45.0%
CGACGACTGATCTTAATCTC+CGG + chr5.4:9428914-9428933 MS.gene015235:CDS 45.0%
GAAACTCCGGTAAGGGTTTA+AGG - chr5.4:9428735-9428754 None:intergenic 45.0%
GGAGATAACTACGTTCACGA+CGG + chr5.4:9429057-9429076 MS.gene015235:CDS 45.0%
TAAACCGAGAAACTCCGGTA+AGG - chr5.4:9428743-9428762 None:intergenic 45.0%
TACCACCAGAACTCATGAAC+CGG - chr5.4:9428855-9428874 None:intergenic 45.0%
TTCAGATGAGTCATCAACGG+TGG - chr5.4:9428779-9428798 None:intergenic 45.0%
! GACTTTTGTGGACAGGAAAC+TGG + chr5.4:9428665-9428684 MS.gene015235:CDS 45.0%
! TTCTGAGGAATCTGTGACGA+CGG + chr5.4:9429008-9429027 MS.gene015235:CDS 45.0%
!! ACCGTTGATGACTCATCTGA+AGG + chr5.4:9428778-9428797 MS.gene015235:CDS 45.0%
!! ATGAACCGGACTTTCTGGTT+CGG - chr5.4:9428841-9428860 None:intergenic 45.0%
AAACTGGCACGTGTGTCAAG+CGG + chr5.4:9428681-9428700 MS.gene015235:CDS 50.0%
AAGTCCGACAGCACCGTTAA+CGG - chr5.4:9428650-9428669 None:intergenic 50.0%
AATTTCCCTCCGGAAACCGT+TGG - chr5.4:9428962-9428981 None:intergenic 50.0%
ACGTGCTTGGAAAGTGGGAT+CGG + chr5.4:9429033-9429052 MS.gene015235:CDS 50.0%
AGAACCGTGAACCCCGTTAA+CGG + chr5.4:9428634-9428653 MS.gene015235:CDS 50.0%
ATCGATACCCCTGAAGCTCA+AGG + chr5.4:9428348-9428367 MS.gene015235:CDS 50.0%
CGGAACCCTTAAACCCTTAC+CGG + chr5.4:9428726-9428745 MS.gene015235:CDS 50.0%
GAATTCATCGACGGTGTTCC+CGG - chr5.4:9428991-9429010 None:intergenic 50.0%
GAGATCCGAACCAGAAAGTC+CGG + chr5.4:9428833-9428852 MS.gene015235:CDS 50.0%
GCGTCTAAACCGAGAAACTC+CGG - chr5.4:9428748-9428767 None:intergenic 50.0%
GGTTGCCGTGTCCTTAGAAA+GGG + chr5.4:9428285-9428304 MS.gene015235:CDS 50.0%
TACCAGGTCTCTGTGGTGAA+GGG - chr5.4:9428440-9428459 None:intergenic 50.0%
TGGCATGACCTTGAGCTTCA+GGG - chr5.4:9428359-9428378 None:intergenic 50.0%
TTACCAGGTCTCTGTGGTGA+AGG - chr5.4:9428441-9428460 None:intergenic 50.0%
!! GGACTTTCTGGTTCGGATCT+CGG - chr5.4:9428834-9428853 None:intergenic 50.0%
ACGCGTTCTGTGGCGTTAAC+CGG - chr5.4:9428936-9428955 None:intergenic 55.0%
ACGGTGACGTGCTTGGAAAG+TGG + chr5.4:9429027-9429046 MS.gene015235:CDS 55.0%
AGCACCGTTAACGGGGTTCA+CGG - chr5.4:9428641-9428660 None:intergenic 55.0%
AGGGTTTAAGGGTTCCGCCA+CGG - chr5.4:9428723-9428742 None:intergenic 55.0%
AGTCCGACAGCACCGTTAAC+GGG - chr5.4:9428649-9428668 None:intergenic 55.0%
CGGTGACGTGCTTGGAAAGT+GGG + chr5.4:9429028-9429047 MS.gene015235:CDS 55.0%
CGGTTGCCGTGTCCTTAGAA+AGG + chr5.4:9428284-9428303 MS.gene015235:CDS 55.0%
GGCATGACCTTGAGCTTCAG+GGG - chr5.4:9428358-9428377 None:intergenic 55.0%
GGTGGTAAACGCAAGCGATC+AGG + chr5.4:9428868-9428887 MS.gene015235:CDS 55.0%
GTCAGCACGGCCGAAAAACT+TGG - chr5.4:9428394-9428413 None:intergenic 55.0%
GTGGCATGACCTTGAGCTTC+AGG - chr5.4:9428360-9428379 None:intergenic 55.0%
GTGTCAAGCGGCGGTTGAAA+CGG + chr5.4:9428693-9428712 MS.gene015235:CDS 55.0%
GTTGACCAACGGTTTCCGGA+GGG + chr5.4:9428954-9428973 MS.gene015235:CDS 55.0%
GTTTCCGGAGGGAAATTCGC+CGG + chr5.4:9428965-9428984 MS.gene015235:CDS 55.0%
TGCTTGGAAAGTGGGATCGG+AGG + chr5.4:9429036-9429055 MS.gene015235:CDS 55.0%
! ACGCGTTGACCAACGGTTTC+CGG + chr5.4:9428950-9428969 MS.gene015235:CDS 55.0%
! CCACAGAACGCGTTGACCAA+CGG + chr5.4:9428943-9428962 MS.gene015235:CDS 55.0%
! GCTGTCGGACTTTTGTGGAC+AGG + chr5.4:9428658-9428677 MS.gene015235:CDS 55.0%
! GTCCGGTTCATGAGTTCTGG+TGG + chr5.4:9428850-9428869 MS.gene015235:CDS 55.0%
!! AACCCCGTTAACGGTGCTGT+CGG + chr5.4:9428643-9428662 MS.gene015235:CDS 55.0%
!!! ACGGTGCTGTCGGACTTTTG+TGG + chr5.4:9428653-9428672 MS.gene015235:CDS 55.0%
AAGTCACGTGCAACGACGCG+CGG + chr5.4:9428803-9428822 MS.gene015235:CDS 60.0%
CCGTTGGTCAACGCGTTCTG+TGG - chr5.4:9428946-9428965 None:intergenic 60.0%
CGTTGACCAACGGTTTCCGG+AGG + chr5.4:9428953-9428972 MS.gene015235:CDS 60.0%
GTCCCTTCACCACAGAGACC+TGG + chr5.4:9428435-9428454 MS.gene015235:CDS 60.0%
GTCCGACAGCACCGTTAACG+GGG - chr5.4:9428648-9428667 None:intergenic 60.0%
GTTGAAACGGTGCTCCGTGG+CGG + chr5.4:9428706-9428725 MS.gene015235:CDS 60.0%
TGTGACGACGGTGACGTGCT+TGG + chr5.4:9429020-9429039 MS.gene015235:CDS 60.0%
CTGGCACGTGTGTCAAGCGG+CGG + chr5.4:9428684-9428703 MS.gene015235:CDS 65.0%
GCGGTTGAAACGGTGCTCCG+TGG + chr5.4:9428703-9428722 MS.gene015235:CDS 65.0%
TCATCGACGGTGTTCCCGGC+CGG - chr5.4:9428987-9429006 None:intergenic 65.0%
CCGGAGGGAAATTCGCCGGC+CGG + chr5.4:9428969-9428988 MS.gene015235:CDS 70.0%
CCGGCCGGCGAATTTCCCTC+CGG - chr5.4:9428972-9428991 None:intergenic 70.0%
CGGAGGGAAATTCGCCGGCC+GGG + chr5.4:9428970-9428989 MS.gene015235:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 9428273 9429110 9428273 ID=MS.gene015235
chr5.4 mRNA 9428273 9429110 9428273 ID=MS.gene015235.t1;Parent=MS.gene015235
chr5.4 exon 9428273 9428456 9428273 ID=MS.gene015235.t1.exon1;Parent=MS.gene015235.t1
chr5.4 CDS 9428273 9428456 9428273 ID=cds.MS.gene015235.t1;Parent=MS.gene015235.t1
chr5.4 exon 9428599 9429110 9428599 ID=MS.gene015235.t1.exon2;Parent=MS.gene015235.t1
chr5.4 CDS 9428599 9429110 9428599 ID=cds.MS.gene015235.t1;Parent=MS.gene015235.t1
Gene Sequence

>MS.gene015235

ATGAGTTGCAACGGTTGCCGTGTCCTTAGAAAGGGTTGTAGTGAGTCATGTATATTACGCCCTTGTATTCAATGGATCGATACCCCTGAAGCTCAAGGTCATGCCACTGTCTTTGTAGCCAAGTTTTTCGGCCGTGCTGACCTTATGTCTTTCATTTCTAACGTCCCTTCACCACAGAGACCTGGTAAATAATCTTTATCTTCTTTTTTTTACTCCACAGTTACACTTTCACTCTAAGTAGTTTGTCACATGATTCTGACAAACTCACTTTGATATAAGCTTTTAGATGAACTAAACTAACTAAGGTTTCTTTTTATGTTATGCAGCTTTGTTTCAATCTTTGTTGTTTGAAGCGTGTGGAAGAACCGTGAACCCCGTTAACGGTGCTGTCGGACTTTTGTGGACAGGAAACTGGCACGTGTGTCAAGCGGCGGTTGAAACGGTGCTCCGTGGCGGAACCCTTAAACCCTTACCGGAGTTTCTCGGTTTAGACGCTCTTACTTCCACCGTTGATGACTCATCTGAAGGAGAAGTCACGTGCAACGACGCGCGGAAGATCCGAGATCCGAACCAGAAAGTCCGGTTCATGAGTTCTGGTGGTAAACGCAAGCGATCAGGTGCTGAAGTTTTGAAGCTTCCGGCGACGACTGATCTTAATCTCCGGTTAACGCCACAGAACGCGTTGACCAACGGTTTCCGGAGGGAAATTCGCCGGCCGGGAACACCGTCGATGAATTCTGAGGAATCTGTGACGACGGTGACGTGCTTGGAAAGTGGGATCGGAGGAGATAACTACGTTCACGACGGAGACCGCAAAGTTCTCAACCTTTTTGTTTGA

Protein sequence

>MS.gene015235.t1

MSCNGCRVLRKGCSESCILRPCIQWIDTPEAQGHATVFVAKFFGRADLMSFISNVPSPQRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLRGGTLKPLPEFLGLDALTSTVDDSSEGEVTCNDARKIRDPNQKVRFMSSGGKRKRSGAEVLKLPATTDLNLRLTPQNALTNGFRREIRRPGTPSMNSEESVTTVTCLESGIGGDNYVHDGDRKVLNLFV