Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011734.t1 | XP_003608484.1 | 97.4 | 233 | 6 | 0 | 1 | 233 | 1 | 233 | 7.40E-128 | 466.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011734.t1 | Q9SZE8 | 57.8 | 249 | 80 | 8 | 1 | 233 | 1 | 240 | 2.6e-65 | 250.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011734.t1 | G7JDY0 | 97.4 | 233 | 6 | 0 | 1 | 233 | 1 | 233 | 5.4e-128 | 466.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene011734.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene011734 | MS.gene015235 | PPI |
| MS.gene011734 | MS.gene57207 | PPI |
| MS.gene011734 | MS.gene09579 | PPI |
| MS.gene20278 | MS.gene011734 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011734.t1 | MTR_4g095600 | 97.425 | 233 | 6 | 0 | 1 | 233 | 1 | 233 | 4.28e-168 | 462 |
| MS.gene011734.t1 | MTR_5g015880 | 70.588 | 238 | 58 | 5 | 1 | 233 | 1 | 231 | 1.93e-110 | 317 |
| MS.gene011734.t1 | MTR_7g007010 | 61.417 | 127 | 46 | 1 | 1 | 124 | 3 | 129 | 1.01e-54 | 177 |
| MS.gene011734.t1 | MTR_6g018270 | 64.545 | 110 | 39 | 0 | 1 | 110 | 3 | 112 | 7.69e-52 | 169 |
| MS.gene011734.t1 | MTR_1g106420 | 62.791 | 129 | 44 | 1 | 1 | 129 | 1 | 125 | 2.13e-51 | 166 |
| MS.gene011734.t1 | MTR_6g453130 | 60.550 | 109 | 41 | 1 | 1 | 107 | 3 | 111 | 1.60e-46 | 154 |
| MS.gene011734.t1 | MTR_1g110840 | 55.000 | 120 | 50 | 2 | 1 | 118 | 3 | 120 | 1.08e-45 | 152 |
| MS.gene011734.t1 | MTR_6g054150 | 50.000 | 120 | 56 | 2 | 1 | 118 | 3 | 120 | 2.03e-41 | 141 |
| MS.gene011734.t1 | MTR_6g054080 | 47.581 | 124 | 61 | 2 | 1 | 122 | 3 | 124 | 2.34e-40 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011734.t1 | AT4G37540 | 57.831 | 249 | 80 | 8 | 1 | 233 | 1 | 240 | 1.72e-82 | 246 |
| MS.gene011734.t1 | AT3G49940 | 57.143 | 252 | 82 | 7 | 1 | 232 | 1 | 246 | 1.53e-81 | 244 |
| MS.gene011734.t1 | AT5G67420 | 53.937 | 254 | 92 | 7 | 1 | 233 | 1 | 250 | 9.63e-75 | 227 |
| MS.gene011734.t1 | AT5G67420 | 53.937 | 254 | 92 | 7 | 1 | 233 | 1 | 250 | 9.63e-75 | 227 |
| MS.gene011734.t1 | AT1G67100 | 66.667 | 111 | 37 | 0 | 1 | 111 | 3 | 113 | 1.05e-53 | 172 |
| MS.gene011734.t1 | AT3G02550 | 47.159 | 176 | 75 | 2 | 1 | 166 | 3 | 170 | 3.71e-51 | 167 |
| MS.gene011734.t1 | AT1G68510 | 63.462 | 104 | 38 | 0 | 1 | 104 | 3 | 106 | 5.53e-48 | 158 |
Find 0 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAACTTTCTCTTATATTTA+CGG | - | 32643:21609-21628 | MS.gene011734:intergenic | 20.0% |
| ! | CGTAAATATAAGAGAAAGTT+TGG | + | 32643:21607-21626 | MS.gene011734:intron | 25.0% |
| AATTAATTACCTGGTCTATG+TGG | - | 32643:21571-21590 | MS.gene011734:intergenic | 30.0% | |
| AGACCAGGTAATTAATTAAC+CGG | + | 32643:21574-21593 | MS.gene011734:intron | 30.0% | |
| ACTGCCTTCTGTTGAAAAAT+TGG | - | 32643:22066-22085 | MS.gene011734:intergenic | 35.0% | |
| AGACCGGTTAATTAATTACC+TGG | - | 32643:21580-21599 | MS.gene011734:intergenic | 35.0% | |
| TCTAGTAATAAGGTGTGTTC+TGG | + | 32643:21970-21989 | MS.gene011734:CDS | 35.0% | |
| ! | GTTTTCGTAGCCAAATTCTT+TGG | + | 32643:21505-21524 | MS.gene011734:CDS | 35.0% |
| !! | AATTTTTCAACAGAAGGCAG+TGG | + | 32643:22065-22084 | MS.gene011734:CDS | 35.0% |
| !! | ATATTTACGGACTCTAAGAC+CGG | - | 32643:21596-21615 | MS.gene011734:intergenic | 35.0% |
| AAATCAAGATTAATCGCCGC+TGG | - | 32643:22033-22052 | MS.gene011734:intergenic | 40.0% | |
| AAGATTCGCGATCCGAATTT+CGG | + | 32643:21931-21950 | MS.gene011734:CDS | 40.0% | |
| GAGCTCACGATCTAGTAATA+AGG | + | 32643:21960-21979 | MS.gene011734:CDS | 40.0% | |
| TAGAAACTGGGATTGGAGAT+CGG | + | 32643:22151-22170 | MS.gene011734:CDS | 40.0% | |
| TTCAGGGACGTTAGAAATGA+AGG | - | 32643:21548-21567 | MS.gene011734:intergenic | 40.0% | |
| ! | TTTTACGACCTTGCTTGCAA+TGG | + | 32643:21449-21468 | MS.gene011734:CDS | 40.0% |
| !! | GACGCCAATTTTTCAACAGA+AGG | + | 32643:22059-22078 | MS.gene011734:CDS | 40.0% |
| ACCAGCTCGACCAAAGAATT+TGG | - | 32643:21518-21537 | MS.gene011734:intergenic | 45.0% | |
| AGAACGGTTAACCCTGTGAA+CGG | + | 32643:21736-21755 | MS.gene011734:CDS | 45.0% | |
| AGATTCGCGATCCGAATTTC+GGG | + | 32643:21932-21951 | MS.gene011734:CDS | 45.0% | |
| ATCGAAACTCCAGAAGCTCA+AGG | + | 32643:21472-21491 | MS.gene011734:CDS | 45.0% | |
| GCGTGTTTAGAAACTGGGAT+TGG | + | 32643:22144-22163 | MS.gene011734:CDS | 45.0% | |
| GTTTCGATCCATTGCAAGCA+AGG | - | 32643:21460-21479 | MS.gene011734:intergenic | 45.0% | |
| TACCTGGTCTATGTGGTTCA+GGG | - | 32643:21564-21583 | MS.gene011734:intergenic | 45.0% | |
| TGCAACCCTTTCGAAGAACT+CGG | - | 32643:21417-21436 | MS.gene011734:intergenic | 45.0% | |
| TTACCTGGTCTATGTGGTTC+AGG | - | 32643:21565-21584 | MS.gene011734:intergenic | 45.0% | |
| TTTATCGGAGCTGACACTTC+TGG | + | 32643:21843-21862 | MS.gene011734:CDS | 45.0% | |
| ! | GACTTCTTTGGACTGGAAAC+TGG | + | 32643:21767-21786 | MS.gene011734:CDS | 45.0% |
| ! | GTTTGAAGCGTGTGGAAGAA+CGG | + | 32643:21720-21739 | MS.gene011734:CDS | 45.0% |
| ! | TCTCTACTGTTTGAAGCGTG+TGG | + | 32643:21712-21731 | MS.gene011734:CDS | 45.0% |
| ! | TTTTCAACAGAAGGCAGTGG+AGG | + | 32643:22068-22087 | MS.gene011734:CDS | 45.0% |
| !! | AGAAGTGTCAGCTCCGATAA+AGG | - | 32643:21844-21863 | MS.gene011734:intergenic | 45.0% |
| !! | AGGTGTGTTCTGGAAGCAAA+CGG | + | 32643:21980-21999 | MS.gene011734:CDS | 45.0% |
| AAACTGGGATTGGAGATCGG+TGG | + | 32643:22154-22173 | MS.gene011734:CDS | 50.0% | |
| ACGACGGCGTGTTTAGAAAC+TGG | + | 32643:22138-22157 | MS.gene011734:CDS | 50.0% | |
| ATTGGAGATCGGTGGAGTCA+CGG | + | 32643:22162-22181 | MS.gene011734:CDS | 50.0% | |
| CGACGGCGTGTTTAGAAACT+GGG | + | 32643:22139-22158 | MS.gene011734:CDS | 50.0% | |
| GAGATCGGAGGAGTTTGTGA+AGG | + | 32643:22005-22024 | MS.gene011734:CDS | 50.0% | |
| GCCAAATTCTTTGGTCGAGC+TGG | + | 32643:21514-21533 | MS.gene011734:CDS | 50.0% | |
| GCTGTTGGACTTCTTTGGAC+TGG | + | 32643:21760-21779 | MS.gene011734:CDS | 50.0% | |
| GGAGTTTGTGAAGGTTCCAG+CGG | + | 32643:22014-22033 | MS.gene011734:CDS | 50.0% | |
| TGCCACTGATGAAGCTTCTG+AGG | + | 32643:21879-21898 | MS.gene011734:CDS | 50.0% | |
| TGGAAGCAAACGGAGGAGAT+CGG | + | 32643:21990-22009 | MS.gene011734:CDS | 50.0% | |
| TGGCACGCTTAGGCCTTTAT+CGG | + | 32643:21828-21847 | MS.gene011734:CDS | 50.0% | |
| TGGCTACGAAAACAGTAGCG+TGG | - | 32643:21498-21517 | MS.gene011734:intergenic | 50.0% | |
| ! | CACTGATGAAGCTTCTGAGG+CGG | + | 32643:21882-21901 | MS.gene011734:CDS | 50.0% |
| !! | ACGGTGCTGTTGGACTTCTT+TGG | + | 32643:21755-21774 | MS.gene011734:CDS | 50.0% |
| !! | ACTGTGGCGCTGATGCTATT+TGG | + | 32643:21908-21927 | MS.gene011734:CDS | 50.0% |
| !! | TGTGTTCTGGAAGCAAACGG+AGG | + | 32643:21983-22002 | MS.gene011734:CDS | 50.0% |
| AAGCAAACGGAGGAGATCGG+AGG | + | 32643:21993-22012 | MS.gene011734:CDS | 55.0% | |
| AGTCCAACAGCACCGTTCAC+AGG | - | 32643:21751-21770 | MS.gene011734:intergenic | 55.0% | |
| CGCCTCAGAAGCTTCATCAG+TGG | - | 32643:21884-21903 | MS.gene011734:intergenic | 55.0% | |
| GATTCCTCCGACGTCATCGA+CGG | - | 32643:22111-22130 | MS.gene011734:intergenic | 55.0% | |
| GCGGTTGAGACAGTTCTTCG+CGG | + | 32643:21805-21824 | MS.gene011734:CDS | 55.0% | |
| GCTTCATCAGTGGCAGTGAG+AGG | - | 32643:21874-21893 | MS.gene011734:intergenic | 55.0% | |
| GTCCAACAGCACCGTTCACA+GGG | - | 32643:21750-21769 | MS.gene011734:intergenic | 55.0% | |
| GTCCCTGAACCACATAGACC+AGG | + | 32643:21559-21578 | MS.gene011734:CDS | 55.0% | |
| GTCGATGACGTCGGAGGAAT+CGG | + | 32643:22110-22129 | MS.gene011734:CDS | 55.0% | |
| GTTGAGACAGTTCTTCGCGG+TGG | + | 32643:21808-21827 | MS.gene011734:CDS | 55.0% | |
| TGGTTCACCGTCGATGACGT+CGG | + | 32643:22101-22120 | MS.gene011734:CDS | 55.0% | |
| !! | AACCCTGTGAACGGTGCTGT+TGG | + | 32643:21745-21764 | MS.gene011734:CDS | 55.0% |
| !! | GGGTGCCGAGTTCTTCGAAA+GGG | + | 32643:21409-21428 | MS.gene011734:CDS | 55.0% |
| !! | TGGGTGCCGAGTTCTTCGAA+AGG | + | 32643:21408-21427 | MS.gene011734:CDS | 55.0% |
| AGAAGGCAGTGGAGGAACGC+CGG | + | 32643:22076-22095 | MS.gene011734:CDS | 60.0% | |
| CTGGCACGTGTGTCAAGCAG+CGG | + | 32643:21786-21805 | MS.gene011734:CDS | 60.0% | |
| GACGTCATCGACGGTGAACC+AGG | - | 32643:22102-22121 | MS.gene011734:intergenic | 60.0% | |
| GAGCTCGCCAGCCCGAAATT+CGG | - | 32643:21946-21965 | MS.gene011734:intergenic | 60.0% | |
| GGAGGAATCGGTGACGACGA+CGG | + | 32643:22122-22141 | MS.gene011734:CDS | 60.0% | |
| GGTGGAGTCACGGTGGTGAT+CGG | + | 32643:22172-22191 | MS.gene011734:CDS | 60.0% | |
| TCATCGACGGTGAACCAGGC+CGG | - | 32643:22098-22117 | MS.gene011734:intergenic | 60.0% | |
| TCGCGATCCGAATTTCGGGC+TGG | + | 32643:21936-21955 | MS.gene011734:CDS | 60.0% | |
| TTCACCGTCGATGACGTCGG+AGG | + | 32643:22104-22123 | MS.gene011734:CDS | 60.0% | |
| TTCTTCGCGGTGGCACGCTT+AGG | + | 32643:21818-21837 | MS.gene011734:CDS | 60.0% | |
| ! | GTAGCGTGGCCTTGAGCTTC+TGG | - | 32643:21484-21503 | MS.gene011734:intergenic | 60.0% |
| !! | GCTTCTGAGGCGGAAGACTG+TGG | + | 32643:21892-21911 | MS.gene011734:CDS | 60.0% |
| GGAGATCGGTGGAGTCACGG+TGG | + | 32643:22165-22184 | MS.gene011734:CDS | 65.0% | |
| GCAGTGGAGGAACGCCGGCC+TGG | + | 32643:22081-22100 | MS.gene011734:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32643 | gene | 21397 | 22218 | 21397 | ID=MS.gene011734 |
| 32643 | mRNA | 21397 | 22218 | 21397 | ID=MS.gene011734.t1;Parent=MS.gene011734 |
| 32643 | exon | 21397 | 21580 | 21397 | ID=MS.gene011734.t1.exon1;Parent=MS.gene011734.t1 |
| 32643 | CDS | 21397 | 21580 | 21397 | ID=cds.MS.gene011734.t1;Parent=MS.gene011734.t1 |
| 32643 | exon | 21701 | 22218 | 21701 | ID=MS.gene011734.t1.exon2;Parent=MS.gene011734.t1 |
| 32643 | CDS | 21701 | 22218 | 21701 | ID=cds.MS.gene011734.t1;Parent=MS.gene011734.t1 |
| Gene Sequence |
| Protein sequence |
