Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041894.t1 | XP_003608484.1 | 98.3 | 233 | 4 | 0 | 1 | 233 | 1 | 233 | 2.30E-129 | 471.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041894.t1 | Q9SZE8 | 57.1 | 252 | 77 | 8 | 1 | 233 | 1 | 240 | 3.4e-65 | 249.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041894.t1 | G7JDY0 | 98.3 | 233 | 4 | 0 | 1 | 233 | 1 | 233 | 1.7e-129 | 471.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene041894.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene20278 | MS.gene041894 | PPI |
| MS.gene041894 | MS.gene015235 | PPI |
| MS.gene041894 | MS.gene57207 | PPI |
| MS.gene09579 | MS.gene041894 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041894.t1 | MTR_4g095600 | 98.283 | 233 | 4 | 0 | 1 | 233 | 1 | 233 | 3.79e-170 | 468 |
| MS.gene041894.t1 | MTR_5g015880 | 71.008 | 238 | 57 | 5 | 1 | 233 | 1 | 231 | 3.11e-111 | 318 |
| MS.gene041894.t1 | MTR_7g007010 | 57.857 | 140 | 54 | 2 | 1 | 135 | 3 | 142 | 1.98e-54 | 177 |
| MS.gene041894.t1 | MTR_1g106420 | 58.042 | 143 | 57 | 2 | 1 | 140 | 1 | 143 | 1.14e-53 | 171 |
| MS.gene041894.t1 | MTR_6g018270 | 55.319 | 141 | 51 | 1 | 1 | 141 | 3 | 131 | 3.39e-52 | 170 |
| MS.gene041894.t1 | MTR_6g453130 | 60.550 | 109 | 41 | 1 | 1 | 107 | 3 | 111 | 2.17e-46 | 154 |
| MS.gene041894.t1 | MTR_1g110840 | 55.000 | 120 | 50 | 2 | 1 | 118 | 3 | 120 | 1.21e-45 | 152 |
| MS.gene041894.t1 | MTR_6g054150 | 51.667 | 120 | 54 | 3 | 1 | 118 | 3 | 120 | 9.68e-42 | 142 |
| MS.gene041894.t1 | MTR_6g054080 | 49.194 | 124 | 59 | 3 | 1 | 122 | 3 | 124 | 1.35e-40 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041894.t1 | AT3G49940 | 57.937 | 252 | 80 | 7 | 1 | 232 | 1 | 246 | 1.27e-83 | 249 |
| MS.gene041894.t1 | AT4G37540 | 57.490 | 247 | 84 | 7 | 1 | 233 | 1 | 240 | 2.03e-82 | 246 |
| MS.gene041894.t1 | AT5G67420 | 54.724 | 254 | 90 | 7 | 1 | 233 | 1 | 250 | 8.14e-77 | 232 |
| MS.gene041894.t1 | AT5G67420 | 54.724 | 254 | 90 | 7 | 1 | 233 | 1 | 250 | 8.14e-77 | 232 |
| MS.gene041894.t1 | AT1G67100 | 67.273 | 110 | 36 | 0 | 1 | 110 | 3 | 112 | 1.13e-53 | 172 |
| MS.gene041894.t1 | AT3G02550 | 49.718 | 177 | 73 | 4 | 1 | 173 | 3 | 167 | 2.63e-52 | 170 |
| MS.gene041894.t1 | AT1G68510 | 61.947 | 113 | 41 | 1 | 1 | 111 | 3 | 115 | 1.09e-48 | 160 |
Find 0 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAACTTTCTCTTATATTTA+CGG | - | 32644:18278-18297 | MS.gene041894:intergenic | 20.0% |
| ! | CGTAAATATAAGAGAAAGTT+TGG | + | 32644:18276-18295 | MS.gene041894:intron | 25.0% |
| AGACCAGGTAATTATTTAAC+CGG | + | 32644:18243-18262 | MS.gene041894:intron | 30.0% | |
| ! | GTTTTCGTAGCAAAATTCTT+TGG | + | 32644:18174-18193 | MS.gene041894:CDS | 30.0% |
| AAATAATTACCTGGTCTCTG+TGG | - | 32644:18240-18259 | MS.gene041894:intergenic | 35.0% | |
| ACTGCCTTCTGTTGAAAAAT+CGG | - | 32644:18735-18754 | MS.gene041894:intergenic | 35.0% | |
| AGACCGGTTAAATAATTACC+TGG | - | 32644:18249-18268 | MS.gene041894:intergenic | 35.0% | |
| TAAATCAAGATTAATCGCCG+CGG | - | 32644:18703-18722 | MS.gene041894:intergenic | 35.0% | |
| TCTAGTAATAAGGTGTGTTC+TGG | + | 32644:18639-18658 | MS.gene041894:CDS | 35.0% | |
| !! | TTTTACGACCTTGTTTGCAA+TGG | + | 32644:18118-18137 | MS.gene041894:CDS | 35.0% |
| AAATCAAGATTAATCGCCGC+GGG | - | 32644:18702-18721 | MS.gene041894:intergenic | 40.0% | |
| AAGATTCGCGATCCGAATTT+CGG | + | 32644:18600-18619 | MS.gene041894:CDS | 40.0% | |
| GAGCTCACGATCTAGTAATA+AGG | + | 32644:18629-18648 | MS.gene041894:CDS | 40.0% | |
| GTTTCGATCCATTGCAAACA+AGG | - | 32644:18129-18148 | MS.gene041894:intergenic | 40.0% | |
| TAGAAACTGGGATTGGAGAT+CGG | + | 32644:18820-18839 | MS.gene041894:CDS | 40.0% | |
| ! | TGCAACCTTTTCGAAGAACT+CGG | - | 32644:18086-18105 | MS.gene041894:intergenic | 40.0% |
| !! | ATATTTACGGACTCTGAGAC+CGG | - | 32644:18265-18284 | MS.gene041894:intergenic | 40.0% |
| !! | GATTTTTCAACAGAAGGCAG+TGG | + | 32644:18734-18753 | MS.gene041894:CDS | 40.0% |
| AGAACGGTTAACCCTGTGAA+CGG | + | 32644:18405-18424 | MS.gene041894:CDS | 45.0% | |
| AGATTCGCGATCCGAATTTC+GGG | + | 32644:18601-18620 | MS.gene041894:CDS | 45.0% | |
| ATCGAAACACCAGAAGCTCA+AGG | + | 32644:18141-18160 | MS.gene041894:CDS | 45.0% | |
| ATTACCTGGTCTCTGTGGTT+CGG | - | 32644:18235-18254 | MS.gene041894:intergenic | 45.0% | |
| GCAAAATTCTTTGGTCGAGC+TGG | + | 32644:18183-18202 | MS.gene041894:CDS | 45.0% | |
| GCGTGTTTAGAAACTGGGAT+TGG | + | 32644:18813-18832 | MS.gene041894:CDS | 45.0% | |
| TTGCTACGAAAACAGTAGCG+TGG | - | 32644:18167-18186 | MS.gene041894:intergenic | 45.0% | |
| ! | GACTTCTTTGGACTGGAAAC+TGG | + | 32644:18436-18455 | MS.gene041894:CDS | 45.0% |
| ! | GTTTGAAGCGTGTGGAAGAA+CGG | + | 32644:18389-18408 | MS.gene041894:CDS | 45.0% |
| ! | TCTCTACTGTTTGAAGCGTG+TGG | + | 32644:18381-18400 | MS.gene041894:CDS | 45.0% |
| ! | TTTTCAACAGAAGGCAGTGG+AGG | + | 32644:18737-18756 | MS.gene041894:CDS | 45.0% |
| !! | AGGTGTGTTCTGGAAGCAAA+CGG | + | 32644:18649-18668 | MS.gene041894:CDS | 45.0% |
| !!! | GACGCCGATTTTTCAACAGA+AGG | + | 32644:18728-18747 | MS.gene041894:CDS | 45.0% |
| AAACTGGGATTGGAGATCGG+TGG | + | 32644:18823-18842 | MS.gene041894:CDS | 50.0% | |
| ACGACGGCGTGTTTAGAAAC+TGG | + | 32644:18807-18826 | MS.gene041894:CDS | 50.0% | |
| ATTGGAGATCGGTGGAGTCA+CGG | + | 32644:18831-18850 | MS.gene041894:CDS | 50.0% | |
| CGACGGCGTGTTTAGAAACT+GGG | + | 32644:18808-18827 | MS.gene041894:CDS | 50.0% | |
| GAGATCGGAGGAGTTTGTCA+AGG | + | 32644:18674-18693 | MS.gene041894:CDS | 50.0% | |
| GCTGTTGGACTTCTTTGGAC+TGG | + | 32644:18429-18448 | MS.gene041894:CDS | 50.0% | |
| TGCCACTGATGAAGCTTCTG+AGG | + | 32644:18548-18567 | MS.gene041894:CDS | 50.0% | |
| TGGAAGCAAACGGAGGAGAT+CGG | + | 32644:18659-18678 | MS.gene041894:CDS | 50.0% | |
| TTACCTGGTCTCTGTGGTTC+GGG | - | 32644:18234-18253 | MS.gene041894:intergenic | 50.0% | |
| TTCGGGGACGTTGGAAATGA+AGG | - | 32644:18217-18236 | MS.gene041894:intergenic | 50.0% | |
| TTGTCAAGCAGCGGTTGAGA+CGG | + | 32644:18464-18483 | MS.gene041894:CDS | 50.0% | |
| TTTACCGGAGCTGACACTTC+TGG | + | 32644:18512-18531 | MS.gene041894:CDS | 50.0% | |
| ! | CACTGATGAAGCTTCTGAGG+CGG | + | 32644:18551-18570 | MS.gene041894:CDS | 50.0% |
| !! | ACGGTGCTGTTGGACTTCTT+TGG | + | 32644:18424-18443 | MS.gene041894:CDS | 50.0% |
| !! | AGAAGTGTCAGCTCCGGTAA+AGG | - | 32644:18513-18532 | MS.gene041894:intergenic | 50.0% |
| !! | TGTGTTCTGGAAGCAAACGG+AGG | + | 32644:18652-18671 | MS.gene041894:CDS | 50.0% |
| AAGCAAACGGAGGAGATCGG+AGG | + | 32644:18662-18681 | MS.gene041894:CDS | 55.0% | |
| AGAAGGCAGTGGAGGAACGA+CGG | + | 32644:18745-18764 | MS.gene041894:CDS | 55.0% | |
| AGTCCAACAGCACCGTTCAC+AGG | - | 32644:18420-18439 | MS.gene041894:intergenic | 55.0% | |
| CGCCTCAGAAGCTTCATCAG+TGG | - | 32644:18553-18572 | MS.gene041894:intergenic | 55.0% | |
| CTGGCACGTTTGTCAAGCAG+CGG | + | 32644:18455-18474 | MS.gene041894:CDS | 55.0% | |
| GAGCTCGCCAACCCGAAATT+CGG | - | 32644:18615-18634 | MS.gene041894:intergenic | 55.0% | |
| GATTCCTCCGACGTCATCGA+CGG | - | 32644:18780-18799 | MS.gene041894:intergenic | 55.0% | |
| GCTTCATCAGTGGCAGTGAG+AGG | - | 32644:18543-18562 | MS.gene041894:intergenic | 55.0% | |
| GGAGTTTGTCAAGGTTCCCG+CGG | + | 32644:18683-18702 | MS.gene041894:CDS | 55.0% | |
| GTCCAACAGCACCGTTCACA+GGG | - | 32644:18419-18438 | MS.gene041894:intergenic | 55.0% | |
| GTCGATGACGTCGGAGGAAT+CGG | + | 32644:18779-18798 | MS.gene041894:CDS | 55.0% | |
| GTTGAGACGGTTCTTCGTGG+TGG | + | 32644:18477-18496 | MS.gene041894:CDS | 55.0% | |
| TACCTGGTCTCTGTGGTTCG+GGG | - | 32644:18233-18252 | MS.gene041894:intergenic | 55.0% | |
| TGGCACGCTTAGGCCTTTAC+CGG | + | 32644:18497-18516 | MS.gene041894:CDS | 55.0% | |
| TGGTTCACCGTCGATGACGT+CGG | + | 32644:18770-18789 | MS.gene041894:CDS | 55.0% | |
| TTCTTCGTGGTGGCACGCTT+AGG | + | 32644:18487-18506 | MS.gene041894:CDS | 55.0% | |
| ! | TCGCGATCCGAATTTCGGGT+TGG | + | 32644:18605-18624 | MS.gene041894:CDS | 55.0% |
| !! | AACCCTGTGAACGGTGCTGT+TGG | + | 32644:18414-18433 | MS.gene041894:CDS | 55.0% |
| !! | GAAGCTTCTGAGGCGGAAGA+CGG | + | 32644:18558-18577 | MS.gene041894:CDS | 55.0% |
| !! | GGGTGCCGAGTTCTTCGAAA+AGG | + | 32644:18078-18097 | MS.gene041894:CDS | 55.0% |
| ACGGTGGCGCTGCTGCTATT+TGG | + | 32644:18577-18596 | MS.gene041894:CDS | 60.0% | |
| GACGTCATCGACGGTGAACC+AGG | - | 32644:18771-18790 | MS.gene041894:intergenic | 60.0% | |
| GCGGTTGAGACGGTTCTTCG+TGG | + | 32644:18474-18493 | MS.gene041894:CDS | 60.0% | |
| GGAGGAATCGGTGACGACGA+CGG | + | 32644:18791-18810 | MS.gene041894:CDS | 60.0% | |
| GGTGGAGTCACGGTGGTGAT+CGG | + | 32644:18841-18860 | MS.gene041894:CDS | 60.0% | |
| TTCACCGTCGATGACGTCGG+AGG | + | 32644:18773-18792 | MS.gene041894:CDS | 60.0% | |
| ! | GTAGCGTGGCCTTGAGCTTC+TGG | - | 32644:18153-18172 | MS.gene041894:intergenic | 60.0% |
| ! | TCTCTGTGGTTCGGGGACGT+TGG | - | 32644:18226-18245 | MS.gene041894:intergenic | 60.0% |
| !! | GCGTCCAGAAGTGTCAGCTC+CGG | - | 32644:18519-18538 | MS.gene041894:intergenic | 60.0% |
| GGAGATCGGTGGAGTCACGG+TGG | + | 32644:18834-18853 | MS.gene041894:CDS | 65.0% | |
| GTCCCCGAACCACAGAGACC+AGG | + | 32644:18228-18247 | MS.gene041894:CDS | 65.0% | |
| !! | GCTTCTGAGGCGGAAGACGG+TGG | + | 32644:18561-18580 | MS.gene041894:CDS | 65.0% |
| GCAGTGGAGGAACGACGGCC+TGG | + | 32644:18750-18769 | MS.gene041894:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32644 | gene | 18066 | 18887 | 18066 | ID=MS.gene041894 |
| 32644 | mRNA | 18066 | 18887 | 18066 | ID=MS.gene041894.t1;Parent=MS.gene041894 |
| 32644 | exon | 18066 | 18249 | 18066 | ID=MS.gene041894.t1.exon1;Parent=MS.gene041894.t1 |
| 32644 | CDS | 18066 | 18249 | 18066 | ID=cds.MS.gene041894.t1;Parent=MS.gene041894.t1 |
| 32644 | exon | 18370 | 18887 | 18370 | ID=MS.gene041894.t1.exon2;Parent=MS.gene041894.t1 |
| 32644 | CDS | 18370 | 18887 | 18370 | ID=cds.MS.gene041894.t1;Parent=MS.gene041894.t1 |
| Gene Sequence |
| Protein sequence |
