AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016205

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016205.t1 MTR_1g016110 100.000 308 0 0 1 308 1 308 0.0 628
MS.gene016205.t1 MTR_1g016110 100.000 308 0 0 1 308 48 355 0.0 625
MS.gene016205.t1 MTR_1g019620 99.351 308 2 0 1 308 48 355 0.0 620
MS.gene016205.t1 MTR_1g070360 34.564 298 143 10 7 296 17 270 2.15e-42 147
MS.gene016205.t1 MTR_6g086600 40.000 180 103 3 8 187 164 338 3.20e-31 122
MS.gene016205.t1 MTR_6g086600 40.000 180 103 3 8 187 173 347 3.34e-31 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016205.t1 AT5G50960 76.721 305 68 2 1 304 48 350 1.52e-172 482
MS.gene016205.t1 AT4G19540 43.005 193 100 5 7 196 38 223 2.56e-38 138
MS.gene016205.t1 AT3G24430 39.444 180 104 3 8 187 172 346 3.00e-31 122

Find 78 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGATTAAATTCAGCATTTC+AGG 0.187756 1.2:+6409349 None:intergenic
CCTTCTAGGCCAAGCATCTT+TGG 0.224706 1.2:+6409850 None:intergenic
GGTGTATCTCTTGACCTTCT+AGG 0.281189 1.2:+6409836 None:intergenic
ATCCAGACGAAGCTGTTATA+TGG 0.325193 1.2:-6409738 MS.gene016205:CDS
TTTATGAAGATTACAGATAA+TGG 0.328286 1.2:-6409424 MS.gene016205:CDS
GAGATGGCGGTACCCTTTCT+TGG 0.328779 1.2:-6409274 MS.gene016205:CDS
AATCTCGGGGTCATGTCAAT+TGG 0.348522 1.2:-6409775 MS.gene016205:CDS
CTTCTAGGCCAAGCATCTTT+GGG 0.359162 1.2:+6409851 None:intergenic
AAAGTTGGAGTGAAAGTTCT+TGG 0.364693 1.2:-6409496 MS.gene016205:CDS
TTTCACAGTGGCCATTCTTT+CGG 0.375809 1.2:+6410005 None:intergenic
CTAATTGTTGATGCTCCGCC+TGG 0.377996 1.2:-6409646 MS.gene016205:CDS
CAGCTAACGCAAATGCCAGC+TGG 0.379548 1.2:+6409926 None:intergenic
CCAAAGATGCTTGGCCTAGA+AGG 0.402010 1.2:-6409850 MS.gene016205:CDS
CCAAGCATCTTTGGGATGCT+TGG 0.406720 1.2:+6409859 None:intergenic
GTCTATGTTGAGTCTAATCT+CGG 0.416769 1.2:-6409790 MS.gene016205:CDS
CACTGTGAAACATAAGATAC+TGG 0.420840 1.2:-6409992 MS.gene016205:CDS
AAGTTGGAGTGAAAGTTCTT+GGG 0.429446 1.2:-6409495 MS.gene016205:CDS
GGCGGTACCCTTTCTTGGTA+AGG 0.430728 1.2:-6409269 MS.gene016205:CDS
CATAAGATACTGGTCTTGTC+AGG 0.432544 1.2:-6409982 MS.gene016205:CDS
TAAAGGAACCTTACCAAGAA+AGG 0.434925 1.2:+6409261 None:intergenic
AGATGTTACGGAGTGGATAT+CGG 0.437420 1.2:-6409392 MS.gene016205:CDS
ACTTGAGATTTGAGATGGGC+TGG 0.443213 1.2:+6409446 None:intergenic
AGCTAACGCAAATGCCAGCT+GGG 0.447107 1.2:+6409927 None:intergenic
CTCCATATAACAGCTTCGTC+TGG 0.454307 1.2:+6409736 None:intergenic
CATAAACTTGAGATTTGAGA+TGG 0.455458 1.2:+6409441 None:intergenic
CGTCTACATTAGCAGCATCT+AGG 0.458881 1.2:+6409590 None:intergenic
CTTCTGGACATTGATATTTG+TGG 0.463857 1.2:-6409883 MS.gene016205:CDS
ATCTCGGGGTCATGTCAATT+GGG 0.470649 1.2:-6409774 MS.gene016205:CDS
CTTTGCAAGGCAGCCGAAGA+AGG 0.473054 1.2:-6409229 MS.gene016205:CDS
TGGTGAGATGAAAGATGTTA+CGG 0.483466 1.2:-6409404 MS.gene016205:CDS
ATGTGTTCATCTGAGGTTCC+AGG 0.485714 1.2:+6409628 None:intergenic
GGCATTTGCGTTAGCTGCAA+GGG 0.486589 1.2:-6409920 MS.gene016205:CDS
GAGACCAACCAAGGTTGCTC+TGG 0.487893 1.2:+6409815 None:intergenic
ACAATCTTTATCTGCAAAGC+AGG 0.488362 1.2:+6409201 None:intergenic
AAAGCAGGATCTGCCTTCTT+CGG 0.495633 1.2:+6409216 None:intergenic
GATTTGAGATGGGCTGGGAC+AGG 0.504393 1.2:+6409452 None:intergenic
TTGCAAAGCTGTGGATCTAA+AGG 0.505094 1.2:+6409244 None:intergenic
GGTTGCCATTGCCGAAAGAA+TGG 0.507040 1.2:-6410016 MS.gene016205:CDS
ATACTGGTCTTGTCAGGAAA+GGG 0.511385 1.2:-6409976 MS.gene016205:CDS
CTTGAGATTTGAGATGGGCT+GGG 0.511450 1.2:+6409447 None:intergenic
GTATTTGATAGTAGCGGTGG+TGG 0.514266 1.2:-6409316 MS.gene016205:CDS
AAAGGAACCTTACCAAGAAA+GGG 0.525559 1.2:+6409262 None:intergenic
TGGCATTTGCGTTAGCTGCA+AGG 0.531152 1.2:-6409921 MS.gene016205:CDS
CCAAGCATCCCAAAGATGCT+TGG 0.531277 1.2:-6409859 MS.gene016205:CDS
TCTATGTTGAGTCTAATCTC+GGG 0.532182 1.2:-6409789 MS.gene016205:CDS
GATAAGCTGATGGAAACTAG+TGG 0.534659 1.2:-6409145 MS.gene016205:CDS
GAGATACACCAGAGCAACCT+TGG 0.541471 1.2:-6409823 MS.gene016205:CDS
GATACTGGTCTTGTCAGGAA+AGG 0.542244 1.2:-6409977 MS.gene016205:CDS
AGTGGCCATTCTTTCGGCAA+TGG 0.546079 1.2:+6410011 None:intergenic
TTGTCAGGAAAGGGAGGTGT+TGG 0.547516 1.2:-6409967 MS.gene016205:CDS
TCGGCTGCCTTGCAAAGCTG+TGG 0.547932 1.2:+6409235 None:intergenic
TACACCAGAGCAACCTTGGT+TGG 0.551299 1.2:-6409819 MS.gene016205:CDS
AGTTGGAGTGAAAGTTCTTG+GGG 0.553652 1.2:-6409494 MS.gene016205:CDS
ATAACAGCTTCGTCTGGATG+AGG 0.554608 1.2:+6409742 None:intergenic
AGATTAGACTCAACATAGAC+AGG 0.558926 1.2:+6409793 None:intergenic
AATTGAAATGTGTTCATCTG+AGG 0.559191 1.2:+6409621 None:intergenic
ATAAACTTGAGATTTGAGAT+GGG 0.559421 1.2:+6409442 None:intergenic
AAAGATAATAGATAAGCTGA+TGG 0.562227 1.2:-6409155 MS.gene016205:CDS
AACTAGTGGGCTGTCAATGA+CGG 0.568788 1.2:-6409131 MS.gene016205:CDS
ACTATCAAATACTTCACTGC+AGG 0.571986 1.2:+6409327 None:intergenic
CTGTCAATGACGGAAAGCAA+TGG 0.575375 1.2:-6409121 MS.gene016205:CDS
GACGAAGCTGTTATATGGAG+AGG 0.584494 1.2:-6409733 MS.gene016205:CDS
ATAAGCTGATGGAAACTAGT+GGG 0.602164 1.2:-6409144 MS.gene016205:CDS
TTTAGATCCACAGCTTTGCA+AGG 0.603851 1.2:-6409242 MS.gene016205:CDS
GTTGAAGATGTGTAACGAGA+TGG 0.614173 1.2:-6409290 MS.gene016205:CDS
AAGAGAGACTTGTTGAGGAG+TGG 0.628280 1.2:+6409552 None:intergenic
AGATGAAAGATGTTACGGAG+TGG 0.628653 1.2:-6409399 MS.gene016205:CDS
CTAGATGCTGCTAATGTAGA+CGG 0.629983 1.2:-6409589 MS.gene016205:CDS
CTGGTCTTGTCAGGAAAGGG+AGG 0.640582 1.2:-6409973 MS.gene016205:CDS
GAAGTATTTGATAGTAGCGG+TGG 0.649921 1.2:-6409319 MS.gene016205:CDS
TCAATAAGAGAGACTTGTTG+AGG 0.650660 1.2:+6409547 None:intergenic
CTATGTTGAGTCTAATCTCG+GGG 0.653466 1.2:-6409788 MS.gene016205:CDS
AGTGAAGTATTTGATAGTAG+CGG 0.680092 1.2:-6409322 MS.gene016205:CDS
GGGGTTGTAGAGAATATGAG+TGG 0.686460 1.2:-6409475 MS.gene016205:CDS
CATAGACAGGAGACCAACCA+AGG 0.702138 1.2:+6409806 None:intergenic
GAAGATGTGTAACGAGATGG+CGG 0.710160 1.2:-6409287 MS.gene016205:CDS
CAGTATCTTATGTTTCACAG+TGG 0.751734 1.2:+6409993 None:intergenic
TGTTCATCTGAGGTTCCAGG+CGG 0.754863 1.2:+6409631 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATGAAGATTACAGATAA+TGG - chr1.2:6409708-6409727 MS.gene016205:CDS 20.0%
!!! AGTTTTTAAAAGACGTTTAT+TGG - chr1.2:6409448-6409467 MS.gene016205:CDS 20.0%
!!! GTTTTTAAAAGACGTTTATT+GGG - chr1.2:6409449-6409468 MS.gene016205:CDS 20.0%
!!! TTTTTAAAAGACGTTTATTG+GGG - chr1.2:6409450-6409469 MS.gene016205:CDS 20.0%
! AAAGATAATAGATAAGCTGA+TGG - chr1.2:6409977-6409996 MS.gene016205:CDS 25.0%
!! ATAAACTTGAGATTTGAGAT+GGG + chr1.2:6409693-6409712 None:intergenic 25.0%
AATTGAAATGTGTTCATCTG+AGG + chr1.2:6409514-6409533 None:intergenic 30.0%
CATAAACTTGAGATTTGAGA+TGG + chr1.2:6409694-6409713 None:intergenic 30.0%
! AGTGAAGTATTTGATAGTAG+CGG - chr1.2:6409810-6409829 MS.gene016205:CDS 30.0%
! GTGAATTTTTGCAAGAAAGT+TGG - chr1.2:6409621-6409640 MS.gene016205:CDS 30.0%
! TCTATTATCTTTTGTAACGC+TGG + chr1.2:6409969-6409988 None:intergenic 30.0%
AAAGGAACCTTACCAAGAAA+GGG + chr1.2:6409873-6409892 None:intergenic 35.0%
AAAGTTGGAGTGAAAGTTCT+TGG - chr1.2:6409636-6409655 MS.gene016205:CDS 35.0%
AAGTTGGAGTGAAAGTTCTT+GGG - chr1.2:6409637-6409656 MS.gene016205:CDS 35.0%
ACAATCTTTATCTGCAAAGC+AGG + chr1.2:6409934-6409953 None:intergenic 35.0%
ACTATCAAATACTTCACTGC+AGG + chr1.2:6409808-6409827 None:intergenic 35.0%
AGATTAGACTCAACATAGAC+AGG + chr1.2:6409342-6409361 None:intergenic 35.0%
ATAAGCTGATGGAAACTAGT+GGG - chr1.2:6409988-6410007 MS.gene016205:CDS 35.0%
CACTGTGAAACATAAGATAC+TGG - chr1.2:6409140-6409159 MS.gene016205:CDS 35.0%
CAGTATCTTATGTTTCACAG+TGG + chr1.2:6409142-6409161 None:intergenic 35.0%
CTTCTGGACATTGATATTTG+TGG - chr1.2:6409249-6409268 MS.gene016205:CDS 35.0%
GCGATTAAATTCAGCATTTC+AGG + chr1.2:6409786-6409805 None:intergenic 35.0%
GTCTATGTTGAGTCTAATCT+CGG - chr1.2:6409342-6409361 MS.gene016205:CDS 35.0%
TAAAGGAACCTTACCAAGAA+AGG + chr1.2:6409874-6409893 None:intergenic 35.0%
TCTATGTTGAGTCTAATCTC+GGG - chr1.2:6409343-6409362 MS.gene016205:CDS 35.0%
TGGTGAGATGAAAGATGTTA+CGG - chr1.2:6409728-6409747 MS.gene016205:CDS 35.0%
!! TCAATAAGAGAGACTTGTTG+AGG + chr1.2:6409588-6409607 None:intergenic 35.0%
AGATGAAAGATGTTACGGAG+TGG - chr1.2:6409733-6409752 MS.gene016205:CDS 40.0%
AGATGTTACGGAGTGGATAT+CGG - chr1.2:6409740-6409759 MS.gene016205:CDS 40.0%
AGTTGGAGTGAAAGTTCTTG+GGG - chr1.2:6409638-6409657 MS.gene016205:CDS 40.0%
ATCCAGACGAAGCTGTTATA+TGG - chr1.2:6409394-6409413 MS.gene016205:CDS 40.0%
CTAGATGCTGCTAATGTAGA+CGG - chr1.2:6409543-6409562 MS.gene016205:CDS 40.0%
CTATGTTGAGTCTAATCTCG+GGG - chr1.2:6409344-6409363 MS.gene016205:CDS 40.0%
GATAAGCTGATGGAAACTAG+TGG - chr1.2:6409987-6410006 MS.gene016205:CDS 40.0%
GTTGAAGATGTGTAACGAGA+TGG - chr1.2:6409842-6409861 MS.gene016205:CDS 40.0%
TTGCAAAGCTGTGGATCTAA+AGG + chr1.2:6409891-6409910 None:intergenic 40.0%
TTTAGATCCACAGCTTTGCA+AGG - chr1.2:6409890-6409909 MS.gene016205:CDS 40.0%
TTTCACAGTGGCCATTCTTT+CGG + chr1.2:6409130-6409149 None:intergenic 40.0%
! GAAGTATTTGATAGTAGCGG+TGG - chr1.2:6409813-6409832 MS.gene016205:CDS 40.0%
!! ATACTGGTCTTGTCAGGAAA+GGG - chr1.2:6409156-6409175 MS.gene016205:CDS 40.0%
!! CATAAGATACTGGTCTTGTC+AGG - chr1.2:6409150-6409169 MS.gene016205:CDS 40.0%
!! TTGATAAGCCCATTTTTGCG+TGG + chr1.2:6409426-6409445 None:intergenic 40.0%
!!! GGATTTTCAAGTTGGTCTTC+TGG - chr1.2:6409233-6409252 MS.gene016205:CDS 40.0%
AAAGCAGGATCTGCCTTCTT+CGG + chr1.2:6409919-6409938 None:intergenic 45.0%
AATCTCGGGGTCATGTCAAT+TGG - chr1.2:6409357-6409376 MS.gene016205:CDS 45.0%
ATAACAGCTTCGTCTGGATG+AGG + chr1.2:6409393-6409412 None:intergenic 45.0%
ATCTCGGGGTCATGTCAATT+GGG - chr1.2:6409358-6409377 MS.gene016205:CDS 45.0%
CGTCTACATTAGCAGCATCT+AGG + chr1.2:6409545-6409564 None:intergenic 45.0%
CTCCATATAACAGCTTCGTC+TGG + chr1.2:6409399-6409418 None:intergenic 45.0%
CTTCTAGGCCAAGCATCTTT+GGG + chr1.2:6409284-6409303 None:intergenic 45.0%
GAAGATGTGTAACGAGATGG+CGG - chr1.2:6409845-6409864 MS.gene016205:CDS 45.0%
GACGAAGCTGTTATATGGAG+AGG - chr1.2:6409399-6409418 MS.gene016205:CDS 45.0%
GGGGTTGTAGAGAATATGAG+TGG - chr1.2:6409657-6409676 MS.gene016205:CDS 45.0%
GGTGTATCTCTTGACCTTCT+AGG + chr1.2:6409299-6409318 None:intergenic 45.0%
! ACTTGAGATTTGAGATGGGC+TGG + chr1.2:6409689-6409708 None:intergenic 45.0%
! ATGTGTTCATCTGAGGTTCC+AGG + chr1.2:6409507-6409526 None:intergenic 45.0%
! CTGTCAATGACGGAAAGCAA+TGG - chr1.2:6410011-6410030 MS.gene016205:CDS 45.0%
! CTTGAGATTTGAGATGGGCT+GGG + chr1.2:6409688-6409707 None:intergenic 45.0%
! GTATTTGATAGTAGCGGTGG+TGG - chr1.2:6409816-6409835 MS.gene016205:CDS 45.0%
!! AACTAGTGGGCTGTCAATGA+CGG - chr1.2:6410001-6410020 MS.gene016205:CDS 45.0%
!! AAGAGAGACTTGTTGAGGAG+TGG + chr1.2:6409583-6409602 None:intergenic 45.0%
!! GATACTGGTCTTGTCAGGAA+AGG - chr1.2:6409155-6409174 MS.gene016205:CDS 45.0%
!!! GCTGCAAGGGATTTTCAAGT+TGG - chr1.2:6409225-6409244 MS.gene016205:CDS 45.0%
AGCTAACGCAAATGCCAGCT+GGG + chr1.2:6409208-6409227 None:intergenic 50.0%
AGTGGCCATTCTTTCGGCAA+TGG + chr1.2:6409124-6409143 None:intergenic 50.0%
CATAGACAGGAGACCAACCA+AGG + chr1.2:6409329-6409348 None:intergenic 50.0%
CCAAAGATGCTTGGCCTAGA+AGG - chr1.2:6409282-6409301 MS.gene016205:CDS 50.0%
CCAAGCATCCCAAAGATGCT+TGG - chr1.2:6409273-6409292 MS.gene016205:CDS 50.0%
CCTTCTAGGCCAAGCATCTT+TGG + chr1.2:6409285-6409304 None:intergenic 50.0%
CTAATTGTTGATGCTCCGCC+TGG - chr1.2:6409486-6409505 MS.gene016205:CDS 50.0%
GAGATACACCAGAGCAACCT+TGG - chr1.2:6409309-6409328 MS.gene016205:CDS 50.0%
GGAGAGGTCCACGCAAAAAT+GGG - chr1.2:6409415-6409434 MS.gene016205:CDS 50.0%
GGCATTTGCGTTAGCTGCAA+GGG - chr1.2:6409212-6409231 MS.gene016205:CDS 50.0%
GGTTGCCATTGCCGAAAGAA+TGG - chr1.2:6409116-6409135 MS.gene016205:CDS 50.0%
TACACCAGAGCAACCTTGGT+TGG - chr1.2:6409313-6409332 MS.gene016205:CDS 50.0%
TGGAGAGGTCCACGCAAAAA+TGG - chr1.2:6409414-6409433 MS.gene016205:CDS 50.0%
TGGCATTTGCGTTAGCTGCA+AGG - chr1.2:6409211-6409230 MS.gene016205:CDS 50.0%
! TGTTCATCTGAGGTTCCAGG+CGG + chr1.2:6409504-6409523 None:intergenic 50.0%
! TTGTCAGGAAAGGGAGGTGT+TGG - chr1.2:6409165-6409184 MS.gene016205:CDS 50.0%
!! CCAAGCATCTTTGGGATGCT+TGG + chr1.2:6409276-6409295 None:intergenic 50.0%
CAGCTAACGCAAATGCCAGC+TGG + chr1.2:6409209-6409228 None:intergenic 55.0%
CTTTGCAAGGCAGCCGAAGA+AGG - chr1.2:6409903-6409922 MS.gene016205:CDS 55.0%
GAGACCAACCAAGGTTGCTC+TGG + chr1.2:6409320-6409339 None:intergenic 55.0%
GGCGGTACCCTTTCTTGGTA+AGG - chr1.2:6409863-6409882 MS.gene016205:CDS 55.0%
! GAGATGGCGGTACCCTTTCT+TGG - chr1.2:6409858-6409877 MS.gene016205:CDS 55.0%
! GAGCACATTTTCTGCCCAGC+TGG - chr1.2:6409191-6409210 MS.gene016205:CDS 55.0%
!! CTGGTCTTGTCAGGAAAGGG+AGG - chr1.2:6409159-6409178 MS.gene016205:CDS 55.0%
!! GATTTGAGATGGGCTGGGAC+AGG + chr1.2:6409683-6409702 None:intergenic 55.0%
TCGGCTGCCTTGCAAAGCTG+TGG + chr1.2:6409900-6409919 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 6409114 6410040 6409114 ID=MS.gene016205
chr1.2 mRNA 6409114 6410040 6409114 ID=MS.gene016205.t1;Parent=MS.gene016205
chr1.2 exon 6409114 6410040 6409114 ID=MS.gene016205.t1.exon1;Parent=MS.gene016205.t1
chr1.2 CDS 6409114 6410040 6409114 ID=cds.MS.gene016205.t1;Parent=MS.gene016205.t1
Gene Sequence

>MS.gene016205

ATGGTTGCCATTGCCGAAAGAATGGCCACTGTGAAACATAAGATACTGGTCTTGTCAGGAAAGGGAGGTGTTGGCAAGAGCACATTTTCTGCCCAGCTGGCATTTGCGTTAGCTGCAAGGGATTTTCAAGTTGGTCTTCTGGACATTGATATTTGTGGTCCAAGCATCCCAAAGATGCTTGGCCTAGAAGGTCAAGAGATACACCAGAGCAACCTTGGTTGGTCTCCTGTCTATGTTGAGTCTAATCTCGGGGTCATGTCAATTGGGTTCATGCTTCCTCATCCAGACGAAGCTGTTATATGGAGAGGTCCACGCAAAAATGGGCTTATCAAGCAGTTTTTAAAAGACGTTTATTGGGGTGAACTTGATTTTCTAATTGTTGATGCTCCGCCTGGAACCTCAGATGAACACATTTCAATTGTTCAATGCCTAGATGCTGCTAATGTAGACGGTGCAATCATAGTTACCACTCCTCAACAAGTCTCTCTTATTGATGTGAAAAAAGAAGTGAATTTTTGCAAGAAAGTTGGAGTGAAAGTTCTTGGGGTTGTAGAGAATATGAGTGGCCTGTCCCAGCCCATCTCAAATCTCAAGTTTATGAAGATTACAGATAATGGTGAGATGAAAGATGTTACGGAGTGGATATCGGAGTACATGAAAGAAAAAGCGCCTGAAATGCTGAATTTAATCGCCTGCAGTGAAGTATTTGATAGTAGCGGTGGTGGAGCGTTGAAGATGTGTAACGAGATGGCGGTACCCTTTCTTGGTAAGGTTCCTTTAGATCCACAGCTTTGCAAGGCAGCCGAAGAAGGCAGATCCTGCTTTGCAGATAAAGATTGTGTTGTGAGCGCTCCAGCGTTACAAAAGATAATAGATAAGCTGATGGAAACTAGTGGGCTGTCAATGACGGAAAGCAATGGAGTGTAG
Protein sequence

>MS.gene016205.t1

MVAIAERMATVKHKILVLSGKGGVGKSTFSAQLAFALAARDFQVGLLDIDICGPSIPKMLGLEGQEIHQSNLGWSPVYVESNLGVMSIGFMLPHPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGTSDEHISIVQCLDAANVDGAIIVTTPQQVSLIDVKKEVNFCKKVGVKVLGVVENMSGLSQPISNLKFMKITDNGEMKDVTEWISEYMKEKAPEMLNLIACSEVFDSSGGGALKMCNEMAVPFLGKVPLDPQLCKAAEEGRSCFADKDCVVSAPALQKIIDKLMETSGLSMTESNGV