AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene019150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019150.t1 MTR_3g449770 79.534 386 22 2 1 329 126 511 0.0 583
MS.gene019150.t1 MTR_7g099540 62.121 132 49 1 133 264 202 332 5.20e-43 154
MS.gene019150.t1 MTR_1g069155 57.639 144 53 2 124 264 259 397 9.86e-42 152
MS.gene019150.t1 MTR_7g099540 59.420 138 49 2 133 264 202 338 1.33e-40 148
MS.gene019150.t1 MTR_1g084980 58.333 144 53 4 124 262 402 543 4.00e-36 138
MS.gene019150.t1 MTR_7g111320 36.076 316 136 12 9 276 241 538 1.24e-29 119
MS.gene019150.t1 MTR_1g019240 58.000 100 38 2 162 259 65 162 4.87e-28 110
MS.gene019150.t1 MTR_7g110810 49.550 111 48 3 158 260 173 283 3.00e-24 102
MS.gene019150.t1 MTR_5g017040 68.571 70 20 1 193 262 114 181 4.16e-23 98.6
MS.gene019150.t1 MTR_1g084980 49.138 116 55 2 124 237 402 515 4.08e-18 85.5
MS.gene019150.t1 MTR_4g131160 52.632 76 31 2 186 256 198 273 4.84e-18 84.7
MS.gene019150.t1 MTR_4g131160 52.632 76 31 2 186 256 201 276 5.58e-18 84.7
MS.gene019150.t1 MTR_1g080890 53.968 63 29 0 186 248 195 257 6.53e-16 78.2
MS.gene019150.t1 MTR_5g005110 41.379 87 44 2 174 253 212 298 7.61e-15 74.7
MS.gene019150.t1 MTR_1g106470 36.559 93 49 2 171 253 178 270 3.74e-13 69.3
MS.gene019150.t1 MTR_3g103030 35.714 98 50 3 186 278 231 320 5.12e-13 69.3
MS.gene019150.t1 MTR_1g106480 44.776 67 35 1 189 253 74 140 8.58e-13 65.9
MS.gene019150.t1 MTR_1g017350 39.450 109 61 3 141 248 221 325 2.19e-12 67.8
MS.gene019150.t1 MTR_1g017350 39.450 109 61 3 141 248 221 325 2.27e-12 67.8
MS.gene019150.t1 MTR_1g017350 39.450 109 61 3 141 248 221 325 2.38e-12 67.8
MS.gene019150.t1 MTR_1g017350 39.450 109 61 3 141 248 221 325 2.41e-12 67.8
MS.gene019150.t1 MTR_1g018090 42.466 73 37 1 186 253 276 348 3.29e-12 67.0
MS.gene019150.t1 MTR_5g048860 51.562 64 30 1 186 248 367 430 3.75e-12 67.4
MS.gene019150.t1 MTR_3g498825 47.945 73 36 2 186 256 149 221 4.71e-12 66.6
MS.gene019150.t1 MTR_7g053410 50.000 64 31 1 186 248 319 382 8.18e-12 66.6
MS.gene019150.t1 MTR_1g106460 37.349 83 37 3 200 277 254 326 8.92e-12 65.5
MS.gene019150.t1 MTR_7g053410 50.000 64 31 1 186 248 319 382 9.08e-12 66.2
MS.gene019150.t1 MTR_3g116770 50.000 64 31 1 186 248 193 256 1.41e-11 65.5
MS.gene019150.t1 MTR_3g116770 50.000 64 31 1 186 248 193 256 1.42e-11 65.5
MS.gene019150.t1 MTR_8g065740 50.000 64 31 1 186 248 307 370 1.53e-11 65.5
MS.gene019150.t1 MTR_8g012290 50.000 64 31 1 186 248 293 356 1.62e-11 65.5
MS.gene019150.t1 MTR_8g012290 50.000 64 31 1 186 248 293 356 1.67e-11 65.5
MS.gene019150.t1 MTR_8g062240 46.875 64 33 1 186 248 153 216 3.46e-11 63.5
MS.gene019150.t1 MTR_8g099880 43.243 74 40 2 186 257 147 220 4.57e-11 63.5
MS.gene019150.t1 MTR_7g069590 34.454 119 60 5 196 305 38 147 5.15e-11 60.8
MS.gene019150.t1 MTR_1g059270 31.132 106 71 2 186 289 162 267 5.37e-11 63.2
MS.gene019150.t1 MTR_4g070320 48.438 64 32 1 186 248 145 208 7.74e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019150.t1 AT3G59060 41.975 324 101 15 66 329 146 442 1.23e-39 145
MS.gene019150.t1 AT3G59060 46.875 256 75 11 66 285 146 376 1.96e-39 144
MS.gene019150.t1 AT3G59060 46.875 256 75 11 66 285 146 376 1.96e-39 144
MS.gene019150.t1 AT3G59060 46.875 256 75 11 66 285 146 376 1.96e-39 144
MS.gene019150.t1 AT2G43010 41.634 257 104 7 57 305 148 366 5.98e-38 140
MS.gene019150.t1 AT2G43010 41.634 257 104 7 57 305 148 366 6.51e-38 140
MS.gene019150.t1 AT2G43010 41.634 257 104 7 57 305 148 366 6.76e-38 140
MS.gene019150.t1 AT2G43010 41.634 257 104 7 57 305 155 373 1.00e-37 140
MS.gene019150.t1 AT2G43010 41.634 257 104 7 57 305 190 408 2.40e-37 139
MS.gene019150.t1 AT2G20180 40.074 272 115 8 26 281 71 310 9.82e-35 131
MS.gene019150.t1 AT2G20180 40.074 272 115 8 26 281 71 310 9.82e-35 131
MS.gene019150.t1 AT2G20180 40.074 272 115 8 26 281 71 310 9.82e-35 131
MS.gene019150.t1 AT2G20180 40.074 272 115 8 26 281 142 381 5.58e-34 130
MS.gene019150.t1 AT2G20180 40.074 272 115 8 26 281 142 381 5.58e-34 130
MS.gene019150.t1 AT2G20180 40.074 272 115 8 26 281 142 381 5.58e-34 130
MS.gene019150.t1 AT2G20180 40.074 272 115 8 26 281 142 381 5.58e-34 130
MS.gene019150.t1 AT1G09530 53.247 154 57 5 120 263 272 420 1.15e-33 130
MS.gene019150.t1 AT1G09530 53.247 154 57 5 120 263 272 420 1.15e-33 130
MS.gene019150.t1 AT1G09530 53.247 154 57 5 120 263 272 420 1.15e-33 130
MS.gene019150.t1 AT1G09530 53.247 154 57 5 120 263 272 420 1.15e-33 130
MS.gene019150.t1 AT1G09530 53.247 154 57 5 120 263 272 420 1.15e-33 130
MS.gene019150.t1 AT1G09530 53.247 154 57 5 120 263 272 420 1.15e-33 130
MS.gene019150.t1 AT2G20180 57.843 102 39 1 147 248 245 342 8.33e-29 114
MS.gene019150.t1 AT5G67110 48.529 136 48 3 125 258 48 163 3.13e-27 106
MS.gene019150.t1 AT4G36930 73.611 72 17 1 190 259 197 268 5.59e-26 107
MS.gene019150.t1 AT2G46970 41.379 174 81 7 125 285 133 298 3.71e-25 104
MS.gene019150.t1 AT2G46970 41.379 174 81 7 125 285 142 307 3.81e-25 105
MS.gene019150.t1 AT2G46970 41.379 174 81 7 125 285 164 329 5.59e-25 104
MS.gene019150.t1 AT4G28790 41.071 168 83 5 119 279 212 370 1.21e-24 103
MS.gene019150.t1 AT4G28800 56.566 99 39 3 183 279 248 344 1.98e-24 103
MS.gene019150.t1 AT4G28815 49.505 101 51 0 183 283 67 167 1.23e-23 97.8
MS.gene019150.t1 AT4G28815 46.087 115 59 1 172 283 129 243 2.96e-23 98.2
MS.gene019150.t1 AT4G00050 44.828 145 50 5 111 246 44 167 3.27e-23 98.2
MS.gene019150.t1 AT4G00050 44.828 145 50 5 111 246 146 269 7.20e-23 98.6
MS.gene019150.t1 AT4G00050 44.828 145 50 5 111 246 146 269 8.52e-23 98.6
MS.gene019150.t1 AT4G28800 55.102 98 36 3 183 279 260 350 1.82e-22 98.2
MS.gene019150.t1 AT2G24260 58.730 63 26 0 186 248 37 99 3.11e-19 85.9
MS.gene019150.t1 AT2G24260 58.730 63 26 0 186 248 140 202 1.45e-18 85.9
MS.gene019150.t1 AT5G58010 54.054 74 31 1 175 248 93 163 1.70e-18 84.7
MS.gene019150.t1 AT4G30980 57.143 63 27 0 186 248 132 194 4.51e-18 84.0
MS.gene019150.t1 AT4G28790 62.903 62 23 0 183 244 270 331 8.65e-18 83.6
MS.gene019150.t1 AT4G30980 57.143 63 27 0 186 248 180 242 1.17e-17 83.2
MS.gene019150.t1 AT3G62090 47.368 95 47 2 179 271 160 253 1.86e-17 82.4
MS.gene019150.t1 AT3G62090 47.368 95 47 2 179 271 177 270 2.32e-17 82.4
MS.gene019150.t1 AT1G26260 33.333 165 92 5 131 277 103 267 7.64e-15 74.7
MS.gene019150.t1 AT1G26260 33.333 165 92 5 131 277 132 296 7.95e-15 75.1
MS.gene019150.t1 AT1G26260 32.934 167 94 5 129 277 152 318 9.17e-15 75.1
MS.gene019150.t1 AT1G26260 32.934 167 94 5 129 277 152 318 9.17e-15 75.1
MS.gene019150.t1 AT1G26260 32.934 167 94 5 129 277 152 318 9.17e-15 75.1
MS.gene019150.t1 AT1G26260 32.934 167 94 5 129 277 152 318 9.17e-15 75.1
MS.gene019150.t1 AT4G28811 44.578 166 73 4 119 278 295 447 1.03e-14 75.5
MS.gene019150.t1 AT4G33880 36.522 115 56 4 167 274 247 351 2.50e-14 73.6
MS.gene019150.t1 AT5G43175 39.506 81 44 1 178 253 126 206 7.32e-14 70.5
MS.gene019150.t1 AT5G61270 46.078 102 53 2 158 258 47 147 1.04e-13 70.9
MS.gene019150.t1 AT5G61270 46.078 102 53 2 158 258 47 147 1.04e-13 70.9
MS.gene019150.t1 AT5G61270 46.078 102 53 2 158 258 135 235 1.14e-13 71.6
MS.gene019150.t1 AT1G27740 37.383 107 58 3 162 262 148 251 1.58e-13 70.1
MS.gene019150.t1 AT2G14760 37.500 104 54 3 161 259 217 314 1.89e-13 70.5
MS.gene019150.t1 AT1G02340 36.667 120 52 3 152 271 113 208 3.53e-13 69.3
MS.gene019150.t1 AT1G02340 36.667 120 52 3 152 271 106 201 3.60e-13 69.3
MS.gene019150.t1 AT2G14760 40.506 79 39 2 186 259 252 327 1.17e-12 68.6
MS.gene019150.t1 AT1G68240 38.182 110 62 3 186 289 63 172 1.22e-12 65.9
MS.gene019150.t1 AT1G68240 38.182 110 62 3 186 289 63 172 1.23e-12 66.2
MS.gene019150.t1 AT5G50915 29.680 219 98 8 109 286 53 256 1.76e-12 67.4
MS.gene019150.t1 AT5G50915 29.680 219 98 8 109 286 53 256 1.76e-12 67.4
MS.gene019150.t1 AT5G50915 29.680 219 98 8 109 286 53 256 2.34e-12 66.6
MS.gene019150.t1 AT5G50915 29.680 219 98 8 109 286 53 256 2.34e-12 66.6
MS.gene019150.t1 AT3G07340 39.216 102 43 3 163 248 225 323 9.08e-12 66.2
MS.gene019150.t1 AT3G07340 39.216 102 43 3 163 248 225 323 1.08e-11 65.9
MS.gene019150.t1 AT5G67110 40.367 109 44 3 125 233 48 135 1.27e-11 62.4
MS.gene019150.t1 AT4G34530 48.438 64 32 1 186 248 174 237 1.34e-11 65.1
MS.gene019150.t1 AT1G68920 50.000 64 31 1 186 248 304 367 1.43e-11 65.9
MS.gene019150.t1 AT1G68920 39.286 112 58 5 186 289 305 414 1.46e-11 65.9
MS.gene019150.t1 AT1G68920 39.286 112 58 5 186 289 305 414 1.46e-11 65.9
MS.gene019150.t1 AT1G68920 39.286 112 58 5 186 289 305 414 1.46e-11 65.9
MS.gene019150.t1 AT5G48560 50.000 64 31 1 186 248 303 366 2.26e-11 65.1
MS.gene019150.t1 AT5G48560 50.000 64 31 1 186 248 303 366 2.55e-11 65.1
MS.gene019150.t1 AT3G23690 33.333 144 87 4 110 248 117 256 2.81e-11 64.3
MS.gene019150.t1 AT1G10120 35.455 110 61 3 140 248 171 271 3.14e-11 64.3
MS.gene019150.t1 AT1G10120 35.455 110 61 3 140 248 171 271 3.14e-11 64.3
MS.gene019150.t1 AT1G59640 28.750 160 96 4 146 299 93 240 4.26e-11 63.2
MS.gene019150.t1 AT4G36540 36.697 109 60 3 148 248 99 206 4.89e-11 63.2
MS.gene019150.t1 AT5G67110 37.500 136 41 4 125 258 48 141 4.90e-11 61.6
MS.gene019150.t1 AT4G36540 36.697 109 60 3 148 248 99 206 4.91e-11 63.2
MS.gene019150.t1 AT5G62610 48.438 64 32 1 186 248 155 218 8.86e-11 62.4
MS.gene019150.t1 AT5G62610 48.438 64 32 1 186 248 155 218 8.86e-11 62.4

Find 76 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTCATCTTCTGGTGGTT+CGG 0.273371 3.3:+36591478 MS.gene019150:CDS
TGATGCTGTGGGTGGCAAAA+TGG 0.294348 3.3:+36593062 MS.gene019150:CDS
TAAATCAGCCGTGAGGAAAA+AGG 0.304657 3.3:+36591699 MS.gene019150:CDS
ATCACTTCAGTTACAACTTC+AGG 0.334793 3.3:+36592179 MS.gene019150:CDS
GGGTATCAAGCAACGGTGTT+TGG 0.343568 3.3:+36591357 MS.gene019150:CDS
CATCTTGATCCTGTTGGACT+TGG 0.357855 3.3:-36591328 None:intergenic
ACTTCATCTTCTGGTGGTTC+GGG 0.366507 3.3:+36591479 MS.gene019150:CDS
GGCATCTCAGCGGTCAGGTT+CGG 0.395919 3.3:+36591720 MS.gene019150:CDS
ATGTTTGCTGTTCCACTCAT+TGG 0.397921 3.3:-36593036 None:intergenic
TGCTTACATCTTGATCCTGT+TGG 0.402600 3.3:-36591334 None:intergenic
GACGGATCCAGAGGCATTCT+CGG 0.403301 3.3:-36592411 None:intergenic
GTCATGACTTCTTGTAGATA+AGG 0.410497 3.3:-36591525 None:intergenic
TTGTTTCTTTGCGATGAATC+GGG 0.417623 3.3:-36591125 None:intergenic
TCAGCACTATATGTCACAAA+TGG 0.421098 3.3:+36592335 MS.gene019150:CDS
GTTGTTTCTTTGCGATGAAT+CGG 0.421412 3.3:-36591126 None:intergenic
ACAATTCCTCGGCATGAGAA+TGG 0.443028 3.3:+36591428 MS.gene019150:CDS
CGGAAGCAACCAAGTCCAAC+AGG 0.451058 3.3:+36591319 MS.gene019150:CDS
CTATATGTCACAAATGGGTA+TGG 0.459276 3.3:+36592341 MS.gene019150:CDS
CAATTCCTCGGCATGAGAAT+GGG 0.468759 3.3:+36591429 MS.gene019150:CDS
AGAAAAGTTTACAACCTTGC+CGG 0.469128 3.3:-36591162 None:intergenic
TTCTGTTAGGTAATGTGGAT+GGG 0.470668 3.3:+36592279 MS.gene019150:intron
CATGGCACCGATGATGCTAC+CGG 0.471237 3.3:+36592308 MS.gene019150:CDS
AACAGGATCAAGATGTAAGC+AGG 0.473432 3.3:+36591336 MS.gene019150:CDS
TCGGCATGAGAATGGGAAAT+CGG 0.473920 3.3:+36591436 MS.gene019150:CDS
TTTCTGTTAGGTAATGTGGA+TGG 0.476764 3.3:+36592278 MS.gene019150:intron
GCAATTACCGAGAATGCCTC+TGG 0.483577 3.3:+36592404 MS.gene019150:CDS
GTAGATGCTAAAGTAACATT+GGG 0.487660 3.3:-36591200 None:intergenic
TAGGTAATGTGGATGGGATC+TGG 0.490326 3.3:+36592285 MS.gene019150:intron
AACAACGGTTTGTGTGGATC+CGG 0.492811 3.3:+36591143 MS.gene019150:CDS
GATGCTGTGGGTGGCAAAAT+GGG 0.501876 3.3:+36593063 MS.gene019150:CDS
GGTGGTTCGGGAAGTAGTCT+TGG 0.502929 3.3:+36591491 MS.gene019150:CDS
GGTAGATGCTAAAGTAACAT+TGG 0.506656 3.3:-36591201 None:intergenic
GGTCCACTGCTATTGCTTGG+TGG 0.511687 3.3:-36593015 None:intergenic
GAATGGGAAATCGGAGATGT+TGG 0.520509 3.3:+36591445 MS.gene019150:CDS
ATTGGTCCACTGCTATTGCT+TGG 0.521152 3.3:-36593018 None:intergenic
ACTCATACCTCACAGTAGTA+AGG 0.524016 3.3:+36591915 MS.gene019150:CDS
CAGCACTATATGTCACAAAT+GGG 0.528954 3.3:+36592336 MS.gene019150:CDS
AGTGCATAATCTTTCAGAAA+GGG 0.530854 3.3:+36591765 MS.gene019150:CDS
GACATATAGTGCTGATATCC+CGG 0.534372 3.3:-36592327 None:intergenic
TCTAAAATTTGCATCGCCGT+TGG 0.537769 3.3:-36591222 None:intergenic
CAAAGCATCAATGTTAGAAG+AGG 0.538478 3.3:+36592140 MS.gene019150:CDS
GGCATTCTCGGTAATTGCAT+CGG 0.543563 3.3:-36592399 None:intergenic
TGAAACCTCGGAGCCGACAT+AGG 0.544656 3.3:-36591092 None:intergenic
TGATATCCCGGTAGCATCAT+CGG 0.547358 3.3:-36592315 None:intergenic
GACAATAGAGAAGTATGTGT+TGG 0.566323 3.3:-36593090 None:intergenic
CAAAGAAACAACGGTTTGTG+TGG 0.571005 3.3:+36591137 MS.gene019150:CDS
CGGTTTGTGTGGATCCGGCA+AGG 0.571774 3.3:+36591148 MS.gene019150:CDS
AGCGGTCAGGTTCGGCTCGT+AGG 0.585796 3.3:+36591728 MS.gene019150:CDS
CCAGAAGATGAAGTAAAAGC+AGG 0.586941 3.3:-36591470 None:intergenic
AAGTGCATAATCTTTCAGAA+AGG 0.591388 3.3:+36591764 MS.gene019150:CDS
TATATGTCACAAATGGGTAT+GGG 0.594345 3.3:+36592342 MS.gene019150:CDS
AGCAAACATTAATGATGCTG+TGG 0.601820 3.3:+36593050 MS.gene019150:CDS
GATTTCCCATTCTCATGCCG+AGG 0.602580 3.3:-36591434 None:intergenic
GATGGATGTTGAAGATTCAG+TGG 0.609243 3.3:+36591562 MS.gene019150:CDS
ATGGCACCGATGATGCTACC+GGG 0.609730 3.3:+36592309 MS.gene019150:CDS
ATGTTCAGAGAACAATTCCT+CGG 0.618230 3.3:+36591417 MS.gene019150:CDS
CTGTGGGTGGCAAAATGGGT+AGG 0.622005 3.3:+36593067 MS.gene019150:CDS
AATGTGGATGGGATCTGGCA+TGG 0.632910 3.3:+36592290 MS.gene019150:CDS
GAGTGTTCGACGATGACAGT+TGG 0.633825 3.3:+36591284 MS.gene019150:CDS
GGAACATGATGATGAAACCT+CGG 0.639628 3.3:-36591104 None:intergenic
ACAGGATCAAGATGTAAGCA+GGG 0.649737 3.3:+36591337 MS.gene019150:CDS
AGATTCAGTGGAACAAAGCG+AGG 0.656069 3.3:+36591574 MS.gene019150:CDS
GTAAGCAGGGTATCAAGCAA+CGG 0.657240 3.3:+36591350 MS.gene019150:CDS
ACAGTTGGTTCGAGTTATTG+CGG 0.658618 3.3:+36591299 MS.gene019150:CDS
GAAGAAACGGACGGATCCAG+AGG 0.660505 3.3:-36592420 None:intergenic
AACATTAATGATGCTGTGGG+TGG 0.661408 3.3:+36593054 MS.gene019150:CDS
AAGTGGAGGAAACGAAGGTG+TGG 0.665117 3.3:-36592370 None:intergenic
AATAGCAGTGGACCAATGAG+TGG 0.668226 3.3:+36593024 MS.gene019150:CDS
ATTCCACCAAGCAATAGCAG+TGG 0.673069 3.3:+36593012 MS.gene019150:CDS
AATTTCCTATGTCGGCTCCG+AGG 0.681257 3.3:+36591087 None:intergenic
GTTACTTTAGCATCTACCAA+CGG 0.684852 3.3:+36591206 MS.gene019150:CDS
GATTCATCGCAAAGAAACAA+CGG 0.698197 3.3:+36591128 MS.gene019150:CDS
GCAAACATTAATGATGCTGT+GGG 0.698935 3.3:+36593051 MS.gene019150:CDS
GATTATGCACTTCAGCAGCG+CGG 0.709911 3.3:-36591753 None:intergenic
TGACAAACCTTACTACTGTG+AGG 0.713524 3.3:-36591922 None:intergenic
CAGAACTTAAATCAGCCGTG+AGG 0.748263 3.3:+36591692 MS.gene019150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATATCATTAATTTATAA+GGG - chr3.3:36592884-36592903 None:intergenic 10.0%
!! ATGAAAACATCTAATTATAA+AGG - chr3.3:36591625-36591644 None:intergenic 15.0%
!! GAATATCATTAATTTATAAG+GGG - chr3.3:36592883-36592902 None:intergenic 15.0%
!! GAGAATATCATTAATTTATA+AGG - chr3.3:36592885-36592904 None:intergenic 15.0%
!!! TTTTTTTTGATTTTTCTGTT+AGG + chr3.3:36592266-36592285 MS.gene019150:intron 15.0%
!! AAGGTAAAATTAATTTCCAT+AGG - chr3.3:36592916-36592935 None:intergenic 20.0%
!! TTGCCTATAAAAAAAAAACA+CGG - chr3.3:36591953-36591972 None:intergenic 20.0%
!!! AAACCGTGTTTTTTTTTTAT+AGG + chr3.3:36591947-36591966 MS.gene019150:intron 20.0%
!!! AAATTTTAGAGACAAAGTTA+CGG + chr3.3:36591235-36591254 MS.gene019150:CDS 20.0%
!!! ATTAATTTTACCTTGTATGT+TGG + chr3.3:36592922-36592941 MS.gene019150:intron 20.0%
!!! ATTTTATGGTTTGTGTTAAT+AGG + chr3.3:36591666-36591685 MS.gene019150:intron 20.0%
! AAAAATTCTTTAGCAATGTG+AGG - chr3.3:36592251-36592270 None:intergenic 25.0%
! AATAAGCTGAAAACAACTTA+TGG - chr3.3:36592687-36592706 None:intergenic 25.0%
! AGACTATTTAGAAGATCTTA+CGG + chr3.3:36592641-36592660 MS.gene019150:intron 25.0%
! ATATGCAAGAGATTATTGTA+TGG - chr3.3:36592220-36592239 None:intergenic 25.0%
!! ACTGTTTTCATGTAAGATAT+AGG - chr3.3:36592744-36592763 None:intergenic 25.0%
!! ATATTCTCATGTTTTACCTA+TGG + chr3.3:36592897-36592916 MS.gene019150:intron 25.0%
!! TAAGTTAGTTTATCCAAACA+GGG + chr3.3:36592840-36592859 MS.gene019150:intron 25.0%
!! TACACTGAGTTATGATTTTA+TGG + chr3.3:36591652-36591671 MS.gene019150:intron 25.0%
!! TTAAGTTAGTTTATCCAAAC+AGG + chr3.3:36592839-36592858 MS.gene019150:intron 25.0%
!!! AATTTTAGAGACAAAGTTAC+GGG + chr3.3:36591236-36591255 MS.gene019150:CDS 25.0%
!!! ACAAAAAATTTGTTAGTAGC+AGG + chr3.3:36591999-36592018 MS.gene019150:intron 25.0%
!!! TGCTAGTGGTTAATTTTTTA+AGG + chr3.3:36591974-36591993 MS.gene019150:intron 25.0%
AAACTATCATGAAGAACTTG+TGG - chr3.3:36592711-36592730 None:intergenic 30.0%
AAGTGCATAATCTTTCAGAA+AGG + chr3.3:36591764-36591783 MS.gene019150:CDS 30.0%
ACTTATGGACATGTCATATA+AGG - chr3.3:36592672-36592691 None:intergenic 30.0%
AGTGCATAATCTTTCAGAAA+GGG + chr3.3:36591765-36591784 MS.gene019150:CDS 30.0%
ATAATCTTTCAGAAAGGGTA+AGG + chr3.3:36591770-36591789 MS.gene019150:intron 30.0%
ATTAAATTGACGCGAATTCA+GGG - chr3.3:36592032-36592051 None:intergenic 30.0%
CAAGAAGTCATGACAAAAAA+AGG + chr3.3:36591534-36591553 MS.gene019150:CDS 30.0%
CCAATCAATATGTACAGATA+TGG + chr3.3:36592958-36592977 MS.gene019150:intron 30.0%
CCATATCTGTACATATTGAT+TGG - chr3.3:36592961-36592980 None:intergenic 30.0%
GCGATTAAATCATACAACAT+GGG - chr3.3:36592078-36592097 None:intergenic 30.0%
GTAGATGCTAAAGTAACATT+GGG - chr3.3:36591203-36591222 None:intergenic 30.0%
TAATCTTTCAGAAAGGGTAA+GGG + chr3.3:36591771-36591790 MS.gene019150:intron 30.0%
TATATGTCACAAATGGGTAT+GGG + chr3.3:36592342-36592361 MS.gene019150:CDS 30.0%
TCTTAAATTTAGACCCTGTT+TGG - chr3.3:36592856-36592875 None:intergenic 30.0%
TGACAAAAAAAGGAAAGTGA+TGG + chr3.3:36591544-36591563 MS.gene019150:CDS 30.0%
TGCGATTAAATCATACAACA+TGG - chr3.3:36592079-36592098 None:intergenic 30.0%
! GATTTTTCTGTTAGGTAATG+TGG + chr3.3:36592274-36592293 MS.gene019150:intron 30.0%
! TTGACTATTTTGTACAGCTT+GGG - chr3.3:36592986-36593005 None:intergenic 30.0%
! TTTGACTATTTTGTACAGCT+TGG - chr3.3:36592987-36593006 None:intergenic 30.0%
! TTTTTATAGGCAATTGCTAG+TGG + chr3.3:36591960-36591979 MS.gene019150:intron 30.0%
!! TTATTGGTTTATTGATGCAG+AGG + chr3.3:36591851-36591870 MS.gene019150:intron 30.0%
!!! ACATCATTTTTGACTTGTTC+AGG - chr3.3:36591401-36591420 None:intergenic 30.0%
AAAATGCTTCTCAGGTACTT+AGG + chr3.3:36592571-36592590 MS.gene019150:intron 35.0%
AAATGCTTCTCAGGTACTTA+GGG + chr3.3:36592572-36592591 MS.gene019150:intron 35.0%
AGAAAAGTTTACAACCTTGC+CGG - chr3.3:36591165-36591184 None:intergenic 35.0%
AGCAAACATTAATGATGCTG+TGG + chr3.3:36593050-36593069 MS.gene019150:CDS 35.0%
AGGTAAAAAGATAGAGTGTG+CGG - chr3.3:36591605-36591624 None:intergenic 35.0%
AGTCTCTCAAGTGTTTATGT+CGG - chr3.3:36592626-36592645 None:intergenic 35.0%
ATCACTTCAGTTACAACTTC+AGG + chr3.3:36592179-36592198 MS.gene019150:CDS 35.0%
ATGTTCAGAGAACAATTCCT+CGG + chr3.3:36591417-36591436 MS.gene019150:CDS 35.0%
CAAAATAGTGCCAACATACA+AGG - chr3.3:36592935-36592954 None:intergenic 35.0%
CAGCACTATATGTCACAAAT+GGG + chr3.3:36592336-36592355 MS.gene019150:CDS 35.0%
CTATATGTCACAAATGGGTA+TGG + chr3.3:36592341-36592360 MS.gene019150:CDS 35.0%
GATTAAATTGACGCGAATTC+AGG - chr3.3:36592033-36592052 None:intergenic 35.0%
GATTCATCGCAAAGAAACAA+CGG + chr3.3:36591128-36591147 MS.gene019150:CDS 35.0%
GCAAACATTAATGATGCTGT+GGG + chr3.3:36593051-36593070 MS.gene019150:CDS 35.0%
GGTAGATGCTAAAGTAACAT+TGG - chr3.3:36591204-36591223 None:intergenic 35.0%
GTCATGACTTCTTGTAGATA+AGG - chr3.3:36591528-36591547 None:intergenic 35.0%
GTTGTATGATTTAATCGCAG+TGG + chr3.3:36592080-36592099 MS.gene019150:intron 35.0%
TCAGCACTATATGTCACAAA+TGG + chr3.3:36592335-36592354 MS.gene019150:CDS 35.0%
TCTTATGCAAAATGCTTCTC+AGG + chr3.3:36592563-36592582 MS.gene019150:intron 35.0%
TTCTGTTAGGTAATGTGGAT+GGG + chr3.3:36592279-36592298 MS.gene019150:intron 35.0%
TTTCTGTTAGGTAATGTGGA+TGG + chr3.3:36592278-36592297 MS.gene019150:intron 35.0%
! CAAAGCATCAATGTTAGAAG+AGG + chr3.3:36592140-36592159 MS.gene019150:CDS 35.0%
! CAGGATTTTGCATCTGATTT+TGG - chr3.3:36592506-36592525 None:intergenic 35.0%
! GACAATAGAGAAGTATGTGT+TGG - chr3.3:36593093-36593112 None:intergenic 35.0%
! GTTACTTTAGCATCTACCAA+CGG + chr3.3:36591206-36591225 MS.gene019150:CDS 35.0%
! TGACTATTTTGTACAGCTTG+GGG - chr3.3:36592985-36593004 None:intergenic 35.0%
! TTGTTTCTTTGCGATGAATC+GGG - chr3.3:36591128-36591147 None:intergenic 35.0%
!! AATTGCATCGGATTTTGAAG+TGG - chr3.3:36592390-36592409 None:intergenic 35.0%
!! CATCTGATTTTGGTAGTTGA+AGG - chr3.3:36592496-36592515 None:intergenic 35.0%
!! GTTGTTTCTTTGCGATGAAT+CGG - chr3.3:36591129-36591148 None:intergenic 35.0%
!! TCGCTTACAAGAAGTGTTAT+TGG + chr3.3:36591835-36591854 MS.gene019150:intron 35.0%
!!! ATTTTTGACTTGTTCAGGCT+CGG - chr3.3:36591396-36591415 None:intergenic 35.0%
AACAGGATCAAGATGTAAGC+AGG + chr3.3:36591336-36591355 MS.gene019150:CDS 40.0%
AACATTAATGATGCTGTGGG+TGG + chr3.3:36593054-36593073 MS.gene019150:CDS 40.0%
ACAGGATCAAGATGTAAGCA+GGG + chr3.3:36591337-36591356 MS.gene019150:CDS 40.0%
ACTCATACCTCACAGTAGTA+AGG + chr3.3:36591915-36591934 MS.gene019150:CDS 40.0%
CAAAGAAACAACGGTTTGTG+TGG + chr3.3:36591137-36591156 MS.gene019150:CDS 40.0%
CCAGAAGATGAAGTAAAAGC+AGG - chr3.3:36591473-36591492 None:intergenic 40.0%
GAGCAATATGCTCGTTACAT+GGG + chr3.3:36592534-36592553 MS.gene019150:intron 40.0%
GATGCAAAATCCTGCTCTTT+CGG + chr3.3:36592512-36592531 MS.gene019150:intron 40.0%
GATGGATGTTGAAGATTCAG+TGG + chr3.3:36591562-36591581 MS.gene019150:CDS 40.0%
TAAATCAGCCGTGAGGAAAA+AGG + chr3.3:36591699-36591718 MS.gene019150:CDS 40.0%
TACTTCATCTTCTGGTGGTT+CGG + chr3.3:36591478-36591497 MS.gene019150:CDS 40.0%
TGACAAACCTTACTACTGTG+AGG - chr3.3:36591925-36591944 None:intergenic 40.0%
TGCTTACATCTTGATCCTGT+TGG - chr3.3:36591337-36591356 None:intergenic 40.0%
! ACGAGCCAAAATCCTGTTTT+AGG + chr3.3:36592468-36592487 MS.gene019150:intron 40.0%
! AGGCACCTAAAACAGGATTT+TGG - chr3.3:36592476-36592495 None:intergenic 40.0%
! GCTTTTACTTCATCTTCTGG+TGG + chr3.3:36591473-36591492 MS.gene019150:CDS 40.0%
! GGAACATGATGATGAAACCT+CGG - chr3.3:36591107-36591126 None:intergenic 40.0%
! TCAGGTACTTAGGGTTTGTT+TGG + chr3.3:36592581-36592600 MS.gene019150:intron 40.0%
! TCTAAAATTTGCATCGCCGT+TGG - chr3.3:36591225-36591244 None:intergenic 40.0%
!! ACAGTTGGTTCGAGTTATTG+CGG + chr3.3:36591299-36591318 MS.gene019150:CDS 40.0%
!! ATGTTTGCTGTTCCACTCAT+TGG - chr3.3:36593039-36593058 None:intergenic 40.0%
!! CCTGCTTTTACTTCATCTTC+TGG + chr3.3:36591470-36591489 MS.gene019150:CDS 40.0%
!! GACATATAGTGCTGATATCC+CGG - chr3.3:36592330-36592349 None:intergenic 40.0%
!! TAGTTGAAGGCACCTAAAAC+AGG - chr3.3:36592483-36592502 None:intergenic 40.0%
!!! GATTTTGGCTCGTTAATGTC+TGG - chr3.3:36592461-36592480 None:intergenic 40.0%
!!! TTTTGAAGTGGAGGAAACGA+AGG - chr3.3:36592378-36592397 None:intergenic 40.0%
AACAACGGTTTGTGTGGATC+CGG + chr3.3:36591143-36591162 MS.gene019150:CDS 45.0%
AATAGCAGTGGACCAATGAG+TGG + chr3.3:36593024-36593043 MS.gene019150:CDS 45.0%
ACAATTCCTCGGCATGAGAA+TGG + chr3.3:36591428-36591447 MS.gene019150:CDS 45.0%
ACTTCATCTTCTGGTGGTTC+GGG + chr3.3:36591479-36591498 MS.gene019150:CDS 45.0%
AGATTCAGTGGAACAAAGCG+AGG + chr3.3:36591574-36591593 MS.gene019150:CDS 45.0%
ATTCCACCAAGCAATAGCAG+TGG + chr3.3:36593012-36593031 MS.gene019150:CDS 45.0%
ATTGGTCCACTGCTATTGCT+TGG - chr3.3:36593021-36593040 None:intergenic 45.0%
CAATTCCTCGGCATGAGAAT+GGG + chr3.3:36591429-36591448 MS.gene019150:CDS 45.0%
CAGAACTTAAATCAGCCGTG+AGG + chr3.3:36591692-36591711 MS.gene019150:CDS 45.0%
CATCTTGATCCTGTTGGACT+TGG - chr3.3:36591331-36591350 None:intergenic 45.0%
GAATGGGAAATCGGAGATGT+TGG + chr3.3:36591445-36591464 MS.gene019150:CDS 45.0%
GGAGCAATATGCTCGTTACA+TGG + chr3.3:36592533-36592552 MS.gene019150:intron 45.0%
GGCATTCTCGGTAATTGCAT+CGG - chr3.3:36592402-36592421 None:intergenic 45.0%
TAGGTAATGTGGATGGGATC+TGG + chr3.3:36592285-36592304 MS.gene019150:intron 45.0%
TCGGCATGAGAATGGGAAAT+CGG + chr3.3:36591436-36591455 MS.gene019150:CDS 45.0%
TGATATCCCGGTAGCATCAT+CGG - chr3.3:36592318-36592337 None:intergenic 45.0%
TGGCTCGTTAATGTCTGGTT+TGG - chr3.3:36592456-36592475 None:intergenic 45.0%
! CTGAGATGCCTTTTTCCTCA+CGG - chr3.3:36591710-36591729 None:intergenic 45.0%
! GTAAGCAGGGTATCAAGCAA+CGG + chr3.3:36591350-36591369 MS.gene019150:CDS 45.0%
!! TGAGGAAAAAGGCATCTCAG+CGG + chr3.3:36591710-36591729 MS.gene019150:CDS 45.0%
!!! TGCATCGGATTTTGAAGTGG+AGG - chr3.3:36592387-36592406 None:intergenic 45.0%
AAGTGGAGGAAACGAAGGTG+TGG - chr3.3:36592373-36592392 None:intergenic 50.0%
AATGTGGATGGGATCTGGCA+TGG + chr3.3:36592290-36592309 MS.gene019150:CDS 50.0%
GAGTGTTCGACGATGACAGT+TGG + chr3.3:36591284-36591303 MS.gene019150:CDS 50.0%
GATGCTGTGGGTGGCAAAAT+GGG + chr3.3:36593063-36593082 MS.gene019150:CDS 50.0%
GATTATGCACTTCAGCAGCG+CGG - chr3.3:36591756-36591775 None:intergenic 50.0%
GATTTCCCATTCTCATGCCG+AGG - chr3.3:36591437-36591456 None:intergenic 50.0%
GCAATTACCGAGAATGCCTC+TGG + chr3.3:36592404-36592423 MS.gene019150:CDS 50.0%
GCATATTGCTCCGAAAGAGC+AGG - chr3.3:36592525-36592544 None:intergenic 50.0%
GGGTATCAAGCAACGGTGTT+TGG + chr3.3:36591357-36591376 MS.gene019150:CDS 50.0%
GTCTGACAGGAAGAAACGGA+CGG - chr3.3:36592432-36592451 None:intergenic 50.0%
TGATGCTGTGGGTGGCAAAA+TGG + chr3.3:36593062-36593081 MS.gene019150:CDS 50.0%
TGGCGTCTGACAGGAAGAAA+CGG - chr3.3:36592436-36592455 None:intergenic 50.0%
!! AAAAAGGCATCTCAGCGGTC+AGG + chr3.3:36591715-36591734 MS.gene019150:CDS 50.0%
CGGAAGCAACCAAGTCCAAC+AGG + chr3.3:36591319-36591338 MS.gene019150:CDS 55.0%
CTGTGGGTGGCAAAATGGGT+AGG + chr3.3:36593067-36593086 MS.gene019150:CDS 55.0%
GAAGAAACGGACGGATCCAG+AGG - chr3.3:36592423-36592442 None:intergenic 55.0%
GGTCCACTGCTATTGCTTGG+TGG - chr3.3:36593018-36593037 None:intergenic 55.0%
! GGTGGTTCGGGAAGTAGTCT+TGG + chr3.3:36591491-36591510 MS.gene019150:CDS 55.0%
!! ATGGCACCGATGATGCTACC+GGG + chr3.3:36592309-36592328 MS.gene019150:CDS 55.0%
!! CATGGCACCGATGATGCTAC+CGG + chr3.3:36592308-36592327 MS.gene019150:CDS 55.0%
!! GACGGATCCAGAGGCATTCT+CGG - chr3.3:36592414-36592433 None:intergenic 55.0%
!! TGTCTGGTTTGGCGTCTGAC+AGG - chr3.3:36592445-36592464 None:intergenic 55.0%
CGGTTTGTGTGGATCCGGCA+AGG + chr3.3:36591148-36591167 MS.gene019150:CDS 60.0%
GGCATCTCAGCGGTCAGGTT+CGG + chr3.3:36591720-36591739 MS.gene019150:CDS 60.0%
! AGCGGTCAGGTTCGGCTCGT+AGG + chr3.3:36591728-36591747 MS.gene019150:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 36591095 36593116 36591095 ID=MS.gene019150
chr3.3 mRNA 36591095 36593116 36591095 ID=MS.gene019150.t1;Parent=MS.gene019150
chr3.3 exon 36591095 36591595 36591095 ID=MS.gene019150.t1.exon1;Parent=MS.gene019150.t1
chr3.3 CDS 36591095 36591595 36591095 ID=cds.MS.gene019150.t1;Parent=MS.gene019150.t1
chr3.3 exon 36591688 36591786 36591688 ID=MS.gene019150.t1.exon2;Parent=MS.gene019150.t1
chr3.3 CDS 36591688 36591786 36591688 ID=cds.MS.gene019150.t1;Parent=MS.gene019150.t1
chr3.3 exon 36591871 36591936 36591871 ID=MS.gene019150.t1.exon3;Parent=MS.gene019150.t1
chr3.3 CDS 36591871 36591936 36591871 ID=cds.MS.gene019150.t1;Parent=MS.gene019150.t1
chr3.3 exon 36592135 36592200 36592135 ID=MS.gene019150.t1.exon4;Parent=MS.gene019150.t1
chr3.3 CDS 36592135 36592200 36592135 ID=cds.MS.gene019150.t1;Parent=MS.gene019150.t1
chr3.3 exon 36592288 36592430 36592288 ID=MS.gene019150.t1.exon5;Parent=MS.gene019150.t1
chr3.3 CDS 36592288 36592430 36592288 ID=cds.MS.gene019150.t1;Parent=MS.gene019150.t1
chr3.3 exon 36592975 36593116 36592975 ID=MS.gene019150.t1.exon6;Parent=MS.gene019150.t1
chr3.3 CDS 36592975 36593116 36592975 ID=cds.MS.gene019150.t1;Parent=MS.gene019150.t1
Gene Sequence

>MS.gene019150

ATGTCGGCTCCGAGGTTTCATCATCATGTTCCCGATTCATCGCAAAGAAACAACGGTTTGTGTGGATCCGGCAAGGTTGTAAACTTTTCTCACTTTTCAAGACTTCCCAATGTTACTTTAGCATCTACCAACGGCGATGCAAATTTTAGAGACAAAGTTACGGGTAATCTGTCACAATGTGATATTAGAGAGTGTTCGACGATGACAGTTGGTTCGAGTTATTGCGGAAGCAACCAAGTCCAACAGGATCAAGATGTAAGCAGGGTATCAAGCAACGGTGTTTGGACTAATTTATCGACCGAGCCTGAACAAGTCAAAAATGATGTTCAGAGAACAATTCCTCGGCATGAGAATGGGAAATCGGAGATGTTGGAACCTGCTTTTACTTCATCTTCTGGTGGTTCGGGAAGTAGTCTTGGAAAAACTTGTTCCTTATCTACAAGAAGTCATGACAAAAAAAGGAAAGTGATGGATGTTGAAGATTCAGTGGAACAAAGCGAGGTAAAGCCGCACACTCTATCTTTTTACCTTTATAATTAGATGTTTTCATTCATTGATACACTGAGTTATGATTTTATGGTTTGTGTTAATAGGATACAGAACTTAAATCAGCCGTGAGGAAAAAGGCATCTCAGCGGTCAGGTTCGGCTCGTAGGAACCGCGCTGCTGAAGTGCATAATCTTTCAGAAAGGGTAAGGGTTTGACACTTCTCTAGATAGACTGAAGTTTTTGAAATTTTTTCGCTTACAAGAAGTGTTATTGGTTTATTGATGCAGAGGCGAAGAGATAGAATTAATGAGAAGATGAAAGCATTACAACAACTCATACCTCACAGTAGTAAGGTTTGTCATCAAACCGTGTTTTTTTTTTATAGGCAATTGCTAGTGGTTAATTTTTTAAGGCGACAAAAAATTTGTTAGTAGCAGGTATTGAACCCTGAATTCGCGTCAATTTAATCGTGTATAGAAAACGATCGATTTCCCATGTTGTATGATTTAATCGCAGTGGTTTAAAAACTGAGTAAGTGATATATATTGCAGACAGACAAAGCATCAATGTTAGAAGAGGCAATTGAATACTTGAAATCACTTCAGTTACAACTTCAGGTATATATATTATGTGCCATACAATAATCTCTTGCATATGAAAATTGCCTCACATTGCTAAAGAATTTTTTTTGATTTTTCTGTTAGGTAATGTGGATGGGATCTGGCATGGCACCGATGATGCTACCGGGATATCAGCACTATATGTCACAAATGGGTATGGGAATGACCACACCTTCGTTTCCTCCACTTCAAAATCCGATGCAATTACCGAGAATGCCTCTGGATCCGTCCGTTTCTTCCTGTCAGACGCCAAACCAGACATTAACGAGCCAAAATCCTGTTTTAGGTGCCTTCAACTACCAAAATCAGATGCAAAATCCTGCTCTTTCGGAGCAATATGCTCGTTACATGGGCTATCATCTTATGCAAAATGCTTCTCAGGTACTTAGGGTTTGTTTGGATTGACTAGTTTTATTCTACCGACATAAACACTTGAGAGACTATTTAGAAGATCTTACGGAAAAACCTTATATGACATGTCCATAAGTTGTTTTCAGCTTATTTCCACAAGTTCTTCATGATAGTTTATAAAAACAGCCTATATCTTACATGAAAACAGTTTTACTTAAATTTTCTCTTTTTGTTACAAAAATAACTTACACATAAACACATACTAGTATATAATAACTATTTAGTTAAGTTAGTTTATCCAAACAGGGTCTAAATTTAAGATGCTCCCCTTATAAATTAATGATATTCTCATGTTTTACCTATGGAAATTAATTTTACCTTGTATGTTGGCACTATTTTGTAGCCAATCAATATGTACAGATATGGTCCCCAAGCTGTACAAAATAGTCAAACAATGATTCCACCAAGCAATAGCAGTGGACCAATGAGTGGAACAGCAAACATTAATGATGCTGTGGGTGGCAAAATGGGTAGGCCAACACATACTTCTCTATTGTCATAG
Protein sequence

>MS.gene019150.t1

MSAPRFHHHVPDSSQRNNGLCGSGKVVNFSHFSRLPNVTLASTNGDANFRDKVTGNLSQCDIRECSTMTVGSSYCGSNQVQQDQDVSRVSSNGVWTNLSTEPEQVKNDVQRTIPRHENGKSEMLEPAFTSSSGGSGSSLGKTCSLSTRSHDKKRKVMDVEDSVEQSEDTELKSAVRKKASQRSGSARRNRAAEVHNLSERRRRDRINEKMKALQQLIPHSSKTDKASMLEEAIEYLKSLQLQLQVMWMGSGMAPMMLPGYQHYMSQMGMGMTTPSFPPLQNPMQLPRMPLDPYGPQAVQNSQTMIPPSNSSGPMSGTANINDAVGGKMGRPTHTSLLS