AlfalfaGEDB | MS.gene023011

M. sativa cultivar XinJiangDaYe / MS.gene023011

KEGG: K02935
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00542.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023011.t1	PNX93291.1	88.9	217	24	0	1	217	1	217	1.30E-97	365.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023011.t1	P53163	36.5	167	96	4	61	217	28	194	1.6e-16	87.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023011.t1	A0A2K3MR37	88.9	217	24	0	1	217	1	217	9.2e-98	365.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene005792	MS.gene023011	PPI
MS.gene023011	MS.gene09988	PPI
MS.gene60517	MS.gene023011	PPI
MS.gene68085	MS.gene023011	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023011.t1	MTR_7g053520	95.853	217	9	0	1	217	1	217	5.26e-144	400
MS.gene023011.t1	MTR_2g064300	86.636	217	28	1	1	217	1	216	4.98e-125	352
MS.gene023011.t1	MTR_2g031760	41.212	165	92	3	54	217	30	190	1.37e-35	124
MS.gene023011.t1	MTR_8g076780	47.368	152	70	2	75	217	45	195	3.77e-32	115
MS.gene023011.t1	MTR_2g031960	48.872	133	63	2	86	217	27	155	2.52e-30	110

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023011.t1	AT1G70190	55.656	221	81	4	1	217	1	208	8.27e-68	207
MS.gene023011.t1	AT1G70190	55.656	221	81	4	1	217	1	208	8.27e-68	207
MS.gene023011.t1	AT4G36420	42.932	191	92	5	31	217	2	179	2.83e-34	120
MS.gene023011.t1	AT3G06040	45.333	150	73	3	69	217	45	186	3.81e-32	115
MS.gene023011.t1	AT3G06040	45.333	150	73	3	69	217	45	186	3.81e-32	115
MS.gene023011.t1	AT3G06040	45.333	150	73	3	69	217	45	186	3.81e-32	115
MS.gene023011.t1	AT4G37660	70.588	68	20	0	150	217	100	167	1.80e-25	97.8
MS.gene023011.t1	AT2G03130	59.494	79	32	0	139	217	13	91	7.48e-16	71.2

Find 50 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTGATGACCTTAATTT+TGG	0.127344	7.2:-49695802	None:intergenic
GAAACCAGGATCTGTAAATT+TGG	0.208162	7.2:+49695683	MS.gene023011:CDS
ACACCTTTCTTTATAATAGA+AGG	0.301571	7.2:-49695882	None:intergenic
TGAAGCAGCTTCCAAAATTA+AGG	0.341436	7.2:+49695791	MS.gene023011:CDS
TCTAGCTGAAGGTGCAGAAT+TGG	0.346308	7.2:+49695605	MS.gene023011:CDS
TTGTCTATACAACAAACTAT+TGG	0.364579	7.2:-49695355	None:intergenic
CCTCTTACTGCTAGCACTCT+AGG	0.374115	7.2:+49695378	MS.gene023011:CDS
CCAGCCAAATTTACAGATCC+TGG	0.386608	7.2:-49695687	None:intergenic
CGATGGAGGCCCACGATGAT+TGG	0.408595	7.2:-49695541	None:intergenic
GAAGGAGGCAAAGGATTTAG+TGG	0.417133	7.2:+49695851	MS.gene023011:CDS
TGTCTATACAACAAACTATT+GGG	0.417171	7.2:-49695354	None:intergenic
CCAGGATCTGTAAATTTGGC+TGG	0.453905	7.2:+49695687	MS.gene023011:CDS
CCTAGGCGCAATTGTCGTTA+TGG	0.458375	7.2:+49695947	MS.gene023011:CDS
CTAGCTGAAGGTGCAGAATT+GGG	0.458679	7.2:+49695606	MS.gene023011:CDS
CCTAGAGTGCTAGCAGTAAG+AGG	0.460957	7.2:-49695378	None:intergenic
ATCTGTAAATTTGGCTGGAA+TGG	0.461697	7.2:+49695692	MS.gene023011:CDS
GGTTTCATAAACCCCACATT+GGG	0.470868	7.2:-49695666	None:intergenic
CATAACGACAATTGCGCCTA+GGG	0.473567	7.2:-49695946	None:intergenic
CTTTCCTTGCAGCTTGACCT+AGG	0.475135	7.2:-49695437	None:intergenic
TTGGTAGGATCAAATGTTGC+AGG	0.479265	7.2:-49695522	None:intergenic
AAAGGTGTTTCGAAGGAAGA+AGG	0.479617	7.2:+49695897	MS.gene023011:CDS
ACACCTTCTATTATAAAGAA+AGG	0.509780	7.2:+49695879	MS.gene023011:CDS
GTTGCAGGATCAAAATCAGT+TGG	0.513514	7.2:-49695507	None:intergenic
CTGAAAACTCTTTCCGATGG+AGG	0.526100	7.2:-49695555	None:intergenic
CCATAACGACAATTGCGCCT+AGG	0.526354	7.2:-49695947	None:intergenic
TTCCAAAATTAAGGTCATCA+AGG	0.526956	7.2:+49695800	MS.gene023011:CDS
TTTCAGGCTTGTTGATGAAG+TGG	0.533852	7.2:+49695572	MS.gene023011:CDS
TATAAAGAAAGGTGTTTCGA+AGG	0.534435	7.2:+49695890	MS.gene023011:CDS
CAATCATCGTGGGCCTCCAT+CGG	0.534976	7.2:+49695542	MS.gene023011:CDS
AAGGTCATCAAGGAGGTTCG+AGG	0.535857	7.2:+49695810	MS.gene023011:CDS
CTCTTACTGCTAGCACTCTA+GGG	0.540669	7.2:+49695379	MS.gene023011:CDS
TTTAGGTTTGAAGGAGGCAA+AGG	0.553743	7.2:+49695842	MS.gene023011:CDS
AATGTATTTACAAGAAACCT+AGG	0.555772	7.2:+49695420	MS.gene023011:CDS
TTGCAGGATCAAAATCAGTT+GGG	0.555773	7.2:-49695506	None:intergenic
ATAAAGGAGATGCCCAATGT+GGG	0.570450	7.2:+49695654	MS.gene023011:CDS
AGCTGCAAGGAAAGATGAAG+AGG	0.574214	7.2:+49695446	MS.gene023011:CDS
AGCCTGAAAACTCTTTCCGA+TGG	0.583898	7.2:-49695558	None:intergenic
GGAGGCCCACGATGATTGGT+AGG	0.590246	7.2:-49695537	None:intergenic
GATAAAGGAGATGCCCAATG+TGG	0.594030	7.2:+49695653	MS.gene023011:CDS
ATAGAGAAATTGAAAGCCCT+AGG	0.602450	7.2:+49695930	MS.gene023011:CDS
TACTGATTTAGGTTTGAAGG+AGG	0.609454	7.2:+49695836	MS.gene023011:CDS
TGGTTTCATAAACCCCACAT+TGG	0.609618	7.2:-49695667	None:intergenic
GAAACCTAGGTCAAGCTGCA+AGG	0.610947	7.2:+49695433	MS.gene023011:CDS
CAAAATTAAGGTCATCAAGG+AGG	0.615684	7.2:+49695803	MS.gene023011:CDS
GCATCTCTAACTCTAGCTGA+AGG	0.625143	7.2:+49695594	MS.gene023011:CDS
AATGTGGGGTTTATGAAACC+AGG	0.637116	7.2:+49695669	MS.gene023011:CDS
AACATCAACAGAAGCCAAGG+AGG	0.649027	7.2:+49695722	MS.gene023011:CDS
TAAAGGAGATGCCCAATGTG+GGG	0.673153	7.2:+49695655	MS.gene023011:CDS
TTGATCCTACCAATCATCGT+GGG	0.673895	7.2:+49695532	MS.gene023011:CDS
TTTGATCCTACCAATCATCG+TGG	0.688414	7.2:+49695531	MS.gene023011:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTCAAATACAGTTTTTTC+TGG	-	chr7.2:49695756-49695775	None:intergenic	20.0%
!	AATGTATTTACAAGAAACCT+AGG	+	chr7.2:49695420-49695439	MS.gene023011:CDS	25.0%
!	ACACCTTCTATTATAAAGAA+AGG	+	chr7.2:49695879-49695898	MS.gene023011:CDS	25.0%
!	ACACCTTTCTTTATAATAGA+AGG	-	chr7.2:49695885-49695904	None:intergenic	25.0%
!	TGTCTATACAACAAACTATT+GGG	-	chr7.2:49695357-49695376	None:intergenic	25.0%
!	TTGTCTATACAACAAACTAT+TGG	-	chr7.2:49695358-49695377	None:intergenic	25.0%
!!!	TTTTACTGATTTAGGTTTGA+AGG	+	chr7.2:49695833-49695852	MS.gene023011:CDS	25.0%
	GATGAAAAAAATGGGGATAA+AGG	+	chr7.2:49695638-49695657	MS.gene023011:CDS	30.0%
	TTCCAAAATTAAGGTCATCA+AGG	+	chr7.2:49695800-49695819	MS.gene023011:CDS	30.0%
!!	TATAAAGAAAGGTGTTTCGA+AGG	+	chr7.2:49695890-49695909	MS.gene023011:CDS	30.0%
!!	TGAGCTTGCTTTTAAGAATA+AGG	+	chr7.2:49695322-49695341	MS.gene023011:CDS	30.0%
	AAAAACATCAACAGAAGCCA+AGG	+	chr7.2:49695719-49695738	MS.gene023011:CDS	35.0%
	ATAGAGAAATTGAAAGCCCT+AGG	+	chr7.2:49695930-49695949	MS.gene023011:CDS	35.0%
	ATCTGTAAATTTGGCTGGAA+TGG	+	chr7.2:49695692-49695711	MS.gene023011:CDS	35.0%
	CAAAATTAAGGTCATCAAGG+AGG	+	chr7.2:49695803-49695822	MS.gene023011:CDS	35.0%
	CTCGCTCTGATGAAAAAAAT+GGG	+	chr7.2:49695630-49695649	MS.gene023011:CDS	35.0%
	GAAACCAGGATCTGTAAATT+TGG	+	chr7.2:49695683-49695702	MS.gene023011:CDS	35.0%
	TCGCTCTGATGAAAAAAATG+GGG	+	chr7.2:49695631-49695650	MS.gene023011:CDS	35.0%
	TCTCGCTCTGATGAAAAAAA+TGG	+	chr7.2:49695629-49695648	MS.gene023011:CDS	35.0%
	TGAAGCAGCTTCCAAAATTA+AGG	+	chr7.2:49695791-49695810	MS.gene023011:CDS	35.0%
	TTGCAGGATCAAAATCAGTT+GGG	-	chr7.2:49695509-49695528	None:intergenic	35.0%
!!	AATTTTGGAAGCTGCTTCAT+AGG	-	chr7.2:49695790-49695809	None:intergenic	35.0%
!!	CTCCTTGATGACCTTAATTT+TGG	-	chr7.2:49695805-49695824	None:intergenic	35.0%
!!	TACTGATTTAGGTTTGAAGG+AGG	+	chr7.2:49695836-49695855	MS.gene023011:CDS	35.0%
!!!	GTTCGAGGTTTTACTGATTT+AGG	+	chr7.2:49695825-49695844	MS.gene023011:CDS	35.0%
	AATGTGGGGTTTATGAAACC+AGG	+	chr7.2:49695669-49695688	MS.gene023011:CDS	40.0%
	ATAAAGGAGATGCCCAATGT+GGG	+	chr7.2:49695654-49695673	MS.gene023011:CDS	40.0%
	GGTTTCATAAACCCCACATT+GGG	-	chr7.2:49695669-49695688	None:intergenic	40.0%
	GTTGCAGGATCAAAATCAGT+TGG	-	chr7.2:49695510-49695529	None:intergenic	40.0%
	TGGTTTCATAAACCCCACAT+TGG	-	chr7.2:49695670-49695689	None:intergenic	40.0%
	TTGATCCTACCAATCATCGT+GGG	+	chr7.2:49695532-49695551	MS.gene023011:CDS	40.0%
	TTGGTAGGATCAAATGTTGC+AGG	-	chr7.2:49695525-49695544	None:intergenic	40.0%
	TTTGATCCTACCAATCATCG+TGG	+	chr7.2:49695531-49695550	MS.gene023011:CDS	40.0%
!	TTTAGGTTTGAAGGAGGCAA+AGG	+	chr7.2:49695842-49695861	MS.gene023011:CDS	40.0%
!!	AAAGGTGTTTCGAAGGAAGA+AGG	+	chr7.2:49695897-49695916	MS.gene023011:CDS	40.0%
!!	TTTCAGGCTTGTTGATGAAG+TGG	+	chr7.2:49695572-49695591	MS.gene023011:CDS	40.0%
	AACATCAACAGAAGCCAAGG+AGG	+	chr7.2:49695722-49695741	MS.gene023011:CDS	45.0%
	AGCCTGAAAACTCTTTCCGA+TGG	-	chr7.2:49695561-49695580	None:intergenic	45.0%
	AGCTGCAAGGAAAGATGAAG+AGG	+	chr7.2:49695446-49695465	MS.gene023011:CDS	45.0%
	CATAACGACAATTGCGCCTA+GGG	-	chr7.2:49695949-49695968	None:intergenic	45.0%
	CCAGCCAAATTTACAGATCC+TGG	-	chr7.2:49695690-49695709	None:intergenic	45.0%
	CCAGGATCTGTAAATTTGGC+TGG	+	chr7.2:49695687-49695706	MS.gene023011:CDS	45.0%
	CTCTTACTGCTAGCACTCTA+GGG	+	chr7.2:49695379-49695398	MS.gene023011:CDS	45.0%
	CTGAAAACTCTTTCCGATGG+AGG	-	chr7.2:49695558-49695577	None:intergenic	45.0%
	GAAGGAGGCAAAGGATTTAG+TGG	+	chr7.2:49695851-49695870	MS.gene023011:CDS	45.0%
	GATAAAGGAGATGCCCAATG+TGG	+	chr7.2:49695653-49695672	MS.gene023011:CDS	45.0%
	GCATCTCTAACTCTAGCTGA+AGG	+	chr7.2:49695594-49695613	MS.gene023011:CDS	45.0%
	TAAAGGAGATGCCCAATGTG+GGG	+	chr7.2:49695655-49695674	MS.gene023011:CDS	45.0%
!	CTAGCTGAAGGTGCAGAATT+GGG	+	chr7.2:49695606-49695625	MS.gene023011:CDS	45.0%
!	CTCCATCGGAAAGAGTTTTC+AGG	+	chr7.2:49695556-49695575	MS.gene023011:CDS	45.0%
!	TCTAGCTGAAGGTGCAGAAT+TGG	+	chr7.2:49695605-49695624	MS.gene023011:CDS	45.0%
	AAGGTCATCAAGGAGGTTCG+AGG	+	chr7.2:49695810-49695829	MS.gene023011:CDS	50.0%
	CCATAACGACAATTGCGCCT+AGG	-	chr7.2:49695950-49695969	None:intergenic	50.0%
	CCTAGGCGCAATTGTCGTTA+TGG	+	chr7.2:49695947-49695966	MS.gene023011:CDS	50.0%
	CCTCTTACTGCTAGCACTCT+AGG	+	chr7.2:49695378-49695397	MS.gene023011:CDS	50.0%
	CTTTCCTTGCAGCTTGACCT+AGG	-	chr7.2:49695440-49695459	None:intergenic	50.0%
	GAAACCTAGGTCAAGCTGCA+AGG	+	chr7.2:49695433-49695452	MS.gene023011:CDS	50.0%
!	CCTAGAGTGCTAGCAGTAAG+AGG	-	chr7.2:49695381-49695400	None:intergenic	50.0%
!!!	TTCTGGCTTTTGCTCCTCCT+TGG	-	chr7.2:49695739-49695758	None:intergenic	50.0%
	CAATCATCGTGGGCCTCCAT+CGG	+	chr7.2:49695542-49695561	MS.gene023011:CDS	55.0%
	CGATGGAGGCCCACGATGAT+TGG	-	chr7.2:49695544-49695563	None:intergenic	60.0%
	GGAGGCCCACGATGATTGGT+AGG	-	chr7.2:49695540-49695559	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	49695321	49695974	49695321	ID=MS.gene023011
chr7.2	mRNA	49695321	49695974	49695321	ID=MS.gene023011.t1;Parent=MS.gene023011
chr7.2	exon	49695321	49695974	49695321	ID=MS.gene023011.t1.exon1;Parent=MS.gene023011.t1
chr7.2	CDS	49695321	49695974	49695321	ID=cds.MS.gene023011.t1;Parent=MS.gene023011.t1

Gene Sequence

>MS.gene023011

ATGAGCTTGCTTTTAAGAATAAGGCATTGTTTACCCAATAGTTTGTTGTATAGACAACCTCTTACTGCTAGCACTCTAGGGCTTAATGCTAGAACATTAAATGTATTTACAAGAAACCTAGGTCAAGCTGCAAGGAAAGATGAAGAGGAAGATGTAGAAGAAGTTGAAATTGATCAACGAAGTCTCCCAACTGATTTTGATCCTGCAACATTTGATCCTACCAATCATCGTGGGCCTCCATCGGAAAGAGTTTTCAGGCTTGTTGATGAAGTGGCATCTCTAACTCTAGCTGAAGGTGCAGAATTGGGTCTCGCTCTGATGAAAAAAATGGGGATAAAGGAGATGCCCAATGTGGGGTTTATGAAACCAGGATCTGTAAATTTGGCTGGAATGGCAGCAAAAACATCAACAGAAGCCAAGGAGGAGCAAAAGCCAGAAAAAACTGTATTTGAATTGAAACTGACGTCCTATGAAGCAGCTTCCAAAATTAAGGTCATCAAGGAGGTTCGAGGTTTTACTGATTTAGGTTTGAAGGAGGCAAAGGATTTAGTGGAAAAAACACCTTCTATTATAAAGAAAGGTGTTTCGAAGGAAGAAGGAGAGCAGATAATAGAGAAATTGAAAGCCCTAGGCGCAATTGTCGTTATGGAATGA

Protein sequence

>MS.gene023011.t1

MSLLLRIRHCLPNSLLYRQPLTASTLGLNARTLNVFTRNLGQAARKDEEEDVEEVEIDQRSLPTDFDPATFDPTNHRGPPSERVFRLVDEVASLTLAEGAELGLALMKKMGIKEMPNVGFMKPGSVNLAGMAAKTSTEAKEEQKPEKTVFELKLTSYEAASKIKVIKEVRGFTDLGLKEAKDLVEKTPSIIKKGVSKEEGEQIIEKLKALGAIVVME