Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60517.t1 | XP_013469320.1 | 97.9 | 536 | 11 | 0 | 1 | 536 | 1 | 536 | 7.10E-283 | 982.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60517.t1 | Q9P4X3 | 42.0 | 421 | 240 | 3 | 23 | 441 | 9 | 427 | 1.2e-94 | 348.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60517.t1 | A0A072VPQ9 | 97.9 | 536 | 11 | 0 | 1 | 536 | 1 | 536 | 5.1e-283 | 982.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60517.t1 | MTR_1g090877 | 97.948 | 536 | 11 | 0 | 1 | 536 | 1 | 536 | 0.0 | 1087 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60517.t1 | AT3G10530 | 66.729 | 535 | 168 | 5 | 4 | 532 | 5 | 535 | 0.0 | 721 |
Find 139 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCTTCTACCAACAAATT+TGG | 0.117506 | 1.2:+68361839 | MS.gene60517:CDS |
| AGTGTTGAGGCTTCAGTTTC+TGG | 0.160612 | 1.2:+68361802 | MS.gene60517:CDS |
| CAGGTTCTGAGGGATGTTTC+TGG | 0.181331 | 1.2:+68362232 | MS.gene60517:CDS |
| ATTTGGGATGCTTCTTTATC+AGG | 0.215020 | 1.2:+68361856 | MS.gene60517:CDS |
| ACAAACCGCTGTAAAGATTT+TGG | 0.230964 | 1.2:-68362775 | None:intergenic |
| TCAGTGGAAGGCTTGTTATT+TGG | 0.239131 | 1.2:-68355229 | None:intergenic |
| TCACTCAAATGGCCACTTAA+TGG | 0.240805 | 1.2:+68362072 | MS.gene60517:CDS |
| CGACCATACGAAGACGTTCT+AGG | 0.250652 | 1.2:+68362325 | MS.gene60517:CDS |
| CGCTGTTTAGGTGTTTCAAA+TGG | 0.263474 | 1.2:-68362427 | None:intergenic |
| GGGAAATATCGAACTGGTTT+AGG | 0.274492 | 1.2:+68361902 | MS.gene60517:CDS |
| TACTTCAAGTGGCCGGTATA+TGG | 0.286386 | 1.2:+68359340 | MS.gene60517:CDS |
| ACTTTGGAGTTTAGTCAAAA+AGG | 0.286444 | 1.2:+68362178 | MS.gene60517:CDS |
| TAAGGGCCACCTAGCAATTA+TGG | 0.314562 | 1.2:+68359376 | MS.gene60517:CDS |
| GAGCCTGATCAGGGAGCTTC+AGG | 0.314653 | 1.2:+68359412 | MS.gene60517:CDS |
| GTTCTCCTGCTCCTGGAATA+AGG | 0.316425 | 1.2:-68362378 | None:intergenic |
| AGGAGAAGCTGCTAATTTAA+AGG | 0.324134 | 1.2:+68355532 | MS.gene60517:CDS |
| ATTGCTAGAGAGAAATTATA+TGG | 0.329371 | 1.2:+68355691 | MS.gene60517:CDS |
| TTGCTTCTACCAACAAATTT+GGG | 0.332802 | 1.2:+68361840 | MS.gene60517:CDS |
| TGGTAGAAGCAAGAAGAAAA+TGG | 0.339608 | 1.2:-68361829 | None:intergenic |
| CATGACTCATTCAATGGTTA+AGG | 0.339749 | 1.2:+68362276 | MS.gene60517:CDS |
| TTGTCTAGTATCCTTATTCC+AGG | 0.340451 | 1.2:+68362367 | MS.gene60517:CDS |
| GAATGAGAAATTACTTTCTC+TGG | 0.348914 | 1.2:-68359879 | None:intergenic |
| GGTATCCATACATATATAAT+CGG | 0.354871 | 1.2:+68361265 | MS.gene60517:intron |
| TACAACGGGGTTGTTTCTTT+AGG | 0.372666 | 1.2:+68361953 | MS.gene60517:CDS |
| GGCTTGCTTGCTTGTGGTAA+TGG | 0.376274 | 1.2:+68362199 | MS.gene60517:CDS |
| TGGAATTAAACCCTTACAAC+GGG | 0.383580 | 1.2:+68361939 | MS.gene60517:CDS |
| CAATGCAAAGAGGCCTTACT+TGG | 0.392464 | 1.2:+68362684 | MS.gene60517:CDS |
| AAAGAAACAACCCCGTTGTA+AGG | 0.402029 | 1.2:-68361950 | None:intergenic |
| TGATGAATCTGAGCCTGATC+AGG | 0.411334 | 1.2:+68359402 | MS.gene60517:CDS |
| GAGAACCCAACTTTGATTCA+TGG | 0.414011 | 1.2:+68362395 | MS.gene60517:CDS |
| ATCTGCTAAAGCTGCTGCTC+AGG | 0.414409 | 1.2:+68355717 | MS.gene60517:CDS |
| AAGAAGCATCCCAAATTTGT+TGG | 0.420273 | 1.2:-68361849 | None:intergenic |
| TCTCACCGTACCGATCTTGC+TGG | 0.422324 | 1.2:-68362512 | None:intergenic |
| GAAGTAAAGTCTATTGTATA+CGG | 0.427102 | 1.2:-68359323 | None:intergenic |
| CCAAAGTTTAGTTATGATGA+TGG | 0.429592 | 1.2:-68362808 | None:intergenic |
| TAACAAGCCTTCCACTGAAC+AGG | 0.438951 | 1.2:+68355234 | MS.gene60517:CDS |
| GTTACAATGGGTACAATAGC+TGG | 0.439170 | 1.2:+68361881 | MS.gene60517:CDS |
| TGCAACCCATGAATCAAAGT+TGG | 0.442723 | 1.2:-68362401 | None:intergenic |
| CAATATGATATTACTTTACC+AGG | 0.444529 | 1.2:+68357019 | MS.gene60517:CDS |
| GTACCTGAAGCTCCCTGATC+AGG | 0.447625 | 1.2:-68359415 | None:intergenic |
| GCTGTTTAGGTGTTTCAAAT+GGG | 0.451780 | 1.2:-68362426 | None:intergenic |
| TCTAGGCATAGGACATTCAA+TGG | 0.452742 | 1.2:+68362342 | MS.gene60517:CDS |
| GCAACCCATGAATCAAAGTT+GGG | 0.456061 | 1.2:-68362400 | None:intergenic |
| GCTCTGGCATTTCACTCAAA+TGG | 0.456207 | 1.2:+68362061 | MS.gene60517:CDS |
| CAGAAACATCCCTCAGAACC+TGG | 0.456648 | 1.2:-68362231 | None:intergenic |
| GTGGAAGGCTTGTTATTTGG+AGG | 0.457414 | 1.2:-68355226 | None:intergenic |
| TTGGGACTTGAGAAAATTTG+AGG | 0.461742 | 1.2:+68362126 | MS.gene60517:CDS |
| CCGTCCCGATTATATATGTA+TGG | 0.461991 | 1.2:-68361270 | None:intergenic |
| TATGGCTATTGGTGGTCGTA+AGG | 0.463097 | 1.2:+68359358 | MS.gene60517:CDS |
| GGATCCAGCATGATTGTCTC+AGG | 0.464997 | 1.2:-68362490 | None:intergenic |
| CTTTATCAGGATGTTACAAT+GGG | 0.469440 | 1.2:+68361869 | MS.gene60517:CDS |
| CTTGACAAGGGGAGACGAAC+TGG | 0.470362 | 1.2:-68362011 | None:intergenic |
| ACCTAAACAGCGAAGAGAAA+AGG | 0.470581 | 1.2:+68362438 | MS.gene60517:CDS |
| TGGTAATGGTTCATATATCC+AGG | 0.471586 | 1.2:+68362213 | MS.gene60517:CDS |
| AGTGGCCGGTATATGGCTAT+TGG | 0.477411 | 1.2:+68359347 | MS.gene60517:CDS |
| CTGGTGCACAGAACTATTCA+AGG | 0.480463 | 1.2:+68362251 | MS.gene60517:CDS |
| GCTAACTGCACAAGAGATAG+AGG | 0.481995 | 1.2:+68362552 | MS.gene60517:CDS |
| ACAATAGACTTTACTTCAAG+TGG | 0.483812 | 1.2:+68359329 | MS.gene60517:CDS |
| AGAACCCAACTTTGATTCAT+GGG | 0.484488 | 1.2:+68362396 | MS.gene60517:CDS |
| AAGGCATAGAAAAGACGTAT+AGG | 0.486312 | 1.2:+68356945 | MS.gene60517:CDS |
| AAGGTACATGACTCATTCAA+TGG | 0.486947 | 1.2:+68362270 | MS.gene60517:CDS |
| CAAGTAAGGCCTCTTTGCAT+TGG | 0.491994 | 1.2:-68362683 | None:intergenic |
| AGCTGCTGTGGAAGCTGTCA+AGG | 0.493903 | 1.2:+68362585 | MS.gene60517:CDS |
| AGGAGCATGGTTCAGTGTTG+AGG | 0.496647 | 1.2:+68361789 | MS.gene60517:intron |
| AAGTTGGGTTCTCCTGCTCC+TGG | 0.498038 | 1.2:-68362385 | None:intergenic |
| GGCCACTTAATGGCTACTGC+CGG | 0.499782 | 1.2:+68362082 | MS.gene60517:CDS |
| AATGAGAAATTACTTTCTCT+GGG | 0.504277 | 1.2:-68359878 | None:intergenic |
| GCCACTTAATGGCTACTGCC+GGG | 0.504950 | 1.2:+68362083 | MS.gene60517:CDS |
| TTGCTGATGAACTAGAACTT+AGG | 0.505406 | 1.2:+68355492 | MS.gene60517:CDS |
| AGCTGCTGCTCAGGCTGAGA+AGG | 0.509056 | 1.2:+68355726 | MS.gene60517:CDS |
| CACAGAGCTTCATTGCCTAA+AGG | 0.511846 | 1.2:+68361293 | MS.gene60517:CDS |
| GTCAAGATGCTATGTCATCA+AGG | 0.517188 | 1.2:+68362028 | MS.gene60517:CDS |
| ACAAAAGGAAGAAGCAAGGC+TGG | 0.517202 | 1.2:+68362628 | MS.gene60517:CDS |
| TCTTTATCAGGATGTTACAA+TGG | 0.517499 | 1.2:+68361868 | MS.gene60517:CDS |
| ACGTGGCCATGCAAATACTT+TGG | 0.518762 | 1.2:+68362162 | MS.gene60517:CDS |
| CTGGTAAAGTAATATCATAT+TGG | 0.522994 | 1.2:-68357018 | None:intergenic |
| AAAGAAACAGAAAACTAATG+AGG | 0.523271 | 1.2:+68362741 | MS.gene60517:CDS |
| AGTATCCTTATTCCAGGAGC+AGG | 0.524714 | 1.2:+68362373 | MS.gene60517:CDS |
| TCAAGGGCCAGTCTCAGCTC+TGG | 0.526804 | 1.2:+68362045 | MS.gene60517:CDS |
| ATAGCTGGGAAATATCGAAC+TGG | 0.530048 | 1.2:+68361896 | MS.gene60517:CDS |
| GGCTGAGATGGAAGCTGCTG+TGG | 0.531233 | 1.2:+68362573 | MS.gene60517:CDS |
| ATTCATACCTGTTCAGTGGA+AGG | 0.532716 | 1.2:-68355241 | None:intergenic |
| ATGACTCATTCAATGGTTAA+GGG | 0.540953 | 1.2:+68362277 | MS.gene60517:CDS |
| CTAGGCATAGGACATTCAAT+GGG | 0.546036 | 1.2:+68362343 | MS.gene60517:CDS |
| AAGAAACAGAAAACTAATGA+GGG | 0.546267 | 1.2:+68362742 | MS.gene60517:CDS |
| TCCACCTGAGACAATCATGC+TGG | 0.557003 | 1.2:+68362486 | MS.gene60517:CDS |
| TTCATATATCCAGGTTCTGA+GGG | 0.557477 | 1.2:+68362222 | MS.gene60517:CDS |
| TGAAATGCCAGAGCTGAGAC+TGG | 0.559422 | 1.2:-68362052 | None:intergenic |
| GTTCATATATCCAGGTTCTG+AGG | 0.561682 | 1.2:+68362221 | MS.gene60517:CDS |
| GAAAACAAAAGGAAGAAGCA+AGG | 0.562476 | 1.2:+68362624 | MS.gene60517:CDS |
| AAGAAACAACCCCGTTGTAA+GGG | 0.563141 | 1.2:-68361949 | None:intergenic |
| ATGGTTAAGGGATACCAGAT+AGG | 0.564220 | 1.2:+68362289 | MS.gene60517:CDS |
| ACAAGAGATAGAGGCTGAGA+TGG | 0.568034 | 1.2:+68362561 | MS.gene60517:CDS |
| CCCCGGCAGTAGCCATTAAG+TGG | 0.570074 | 1.2:-68362084 | None:intergenic |
| GGCCGGTATATGGCTATTGG+TGG | 0.570218 | 1.2:+68359350 | MS.gene60517:CDS |
| GTATCCATACATATATAATC+GGG | 0.570684 | 1.2:+68361266 | MS.gene60517:CDS |
| TTACAATGGGTACAATAGCT+GGG | 0.572680 | 1.2:+68361882 | MS.gene60517:CDS |
| TGGATTATGCCTAGCGAAGG+AGG | 0.572860 | 1.2:+68356908 | MS.gene60517:CDS |
| ATGCCTAGAACGTCTTCGTA+TGG | 0.573287 | 1.2:-68362328 | None:intergenic |
| GACCACCAATAGCCATATAC+CGG | 0.573429 | 1.2:-68359352 | None:intergenic |
| TCATCATGTCCATAATTGCT+AGG | 0.576850 | 1.2:-68359385 | None:intergenic |
| TCTATATAACCTCCTTCGCT+AGG | 0.576884 | 1.2:-68356917 | None:intergenic |
| CTCACCGTACCGATCTTGCT+GGG | 0.578578 | 1.2:-68362511 | None:intergenic |
| GTTTCTTTAGGCCACTCAGC+CGG | 0.578772 | 1.2:+68361965 | MS.gene60517:CDS |
| ATGGCTATTGGTGGTCGTAA+GGG | 0.579219 | 1.2:+68359359 | MS.gene60517:CDS |
| ATGCTGGATCCCAGCAAGAT+CGG | 0.579275 | 1.2:+68362502 | MS.gene60517:CDS |
| GCTTGAGATATCTACTTCAC+CGG | 0.581565 | 1.2:-68356987 | None:intergenic |
| AAAGACGTATAGGATTAAGC+AGG | 0.582780 | 1.2:+68356955 | MS.gene60517:CDS |
| ACAAGGGGAGACGAACTGGT+TGG | 0.583473 | 1.2:-68362007 | None:intergenic |
| TAGACTTTACTTCAAGTGGC+CGG | 0.590973 | 1.2:+68359333 | MS.gene60517:CDS |
| TCCAGCATGATTGTCTCAGG+TGG | 0.597209 | 1.2:-68362487 | None:intergenic |
| AAGTAGATATCTCAAGCTCA+AGG | 0.603892 | 1.2:+68356993 | MS.gene60517:CDS |
| TTTAGGACGCACAAATGTGA+TGG | 0.605701 | 1.2:+68361919 | MS.gene60517:CDS |
| TAGCGAAGGAGGTTATATAG+AGG | 0.605779 | 1.2:+68356919 | MS.gene60517:CDS |
| GATGAATCTGAGCCTGATCA+GGG | 0.606259 | 1.2:+68359403 | MS.gene60517:CDS |
| CTTCCACATAGTAACTGTAC+CGG | 0.607488 | 1.2:-68361984 | None:intergenic |
| TCAAGATGCTATGTCATCAA+GGG | 0.609077 | 1.2:+68362029 | MS.gene60517:CDS |
| TAAACTCCAAAGTATTTGCA+TGG | 0.609945 | 1.2:-68362168 | None:intergenic |
| ATGACATAGCATCTTGACAA+GGG | 0.617617 | 1.2:-68362023 | None:intergenic |
| CCACTTAATGGCTACTGCCG+GGG | 0.620121 | 1.2:+68362084 | MS.gene60517:CDS |
| AAATATTCATACCTGTTCAG+TGG | 0.624277 | 1.2:-68355245 | None:intergenic |
| GCTGCTGTGGAAGCTGTCAA+GGG | 0.626643 | 1.2:+68362586 | MS.gene60517:CDS |
| ATGCAATTGCCAATGCAAAG+AGG | 0.626680 | 1.2:+68362674 | MS.gene60517:CDS |
| CAGTGGATTATGCCTAGCGA+AGG | 0.626814 | 1.2:+68356905 | MS.gene60517:intron |
| GGATCCCAGCAAGATCGGTA+CGG | 0.626856 | 1.2:+68362507 | MS.gene60517:CDS |
| ATTAAGCAGGAGACAATAGC+CGG | 0.629818 | 1.2:+68356968 | MS.gene60517:CDS |
| TACGAAGACGTTCTAGGCAT+AGG | 0.632328 | 1.2:+68362331 | MS.gene60517:CDS |
| AACTGCCAAAATCTTTACAG+CGG | 0.639793 | 1.2:+68362770 | MS.gene60517:CDS |
| GGAGGTTATATAGAGGCTGA+AGG | 0.643831 | 1.2:+68356926 | MS.gene60517:CDS |
| CAGCCGGTACAGTTACTATG+TGG | 0.648457 | 1.2:+68361981 | MS.gene60517:CDS |
| ATGGAATTAAACCCTTACAA+CGG | 0.650725 | 1.2:+68361938 | MS.gene60517:CDS |
| AAGAGAAACTGTGCGTGATG+TGG | 0.656485 | 1.2:+68359822 | MS.gene60517:CDS |
| TAGTAACTGTACCGGCTGAG+TGG | 0.670354 | 1.2:-68361976 | None:intergenic |
| CCATACATATATAATCGGGA+CGG | 0.677850 | 1.2:+68361270 | MS.gene60517:CDS |
| AGGTCAAAGAAGTATCTCAG+AGG | 0.688390 | 1.2:+68355512 | MS.gene60517:CDS |
| TGACATAGCATCTTGACAAG+GGG | 0.690641 | 1.2:-68362022 | None:intergenic |
| TCATGTCCATAATTGCTAGG+TGG | 0.709708 | 1.2:-68359382 | None:intergenic |
| GATGACATAGCATCTTGACA+AGG | 0.739903 | 1.2:-68362024 | None:intergenic |
| GGAATTAAACCCTTACAACG+GGG | 0.772754 | 1.2:+68361940 | MS.gene60517:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 68355199 | 68362819 | 68355199 | ID=MS.gene60517 |
| chr1.2 | mRNA | 68355199 | 68362819 | 68355199 | ID=MS.gene60517.t1;Parent=MS.gene60517 |
| chr1.2 | exon | 68355199 | 68355255 | 68355199 | ID=MS.gene60517.t1.exon1;Parent=MS.gene60517.t1 |
| chr1.2 | CDS | 68355199 | 68355255 | 68355199 | ID=cds.MS.gene60517.t1;Parent=MS.gene60517.t1 |
| chr1.2 | exon | 68355488 | 68355553 | 68355488 | ID=MS.gene60517.t1.exon2;Parent=MS.gene60517.t1 |
| chr1.2 | CDS | 68355488 | 68355553 | 68355488 | ID=cds.MS.gene60517.t1;Parent=MS.gene60517.t1 |
| chr1.2 | exon | 68355658 | 68355747 | 68355658 | ID=MS.gene60517.t1.exon3;Parent=MS.gene60517.t1 |
| chr1.2 | CDS | 68355658 | 68355747 | 68355658 | ID=cds.MS.gene60517.t1;Parent=MS.gene60517.t1 |
| chr1.2 | exon | 68356908 | 68357040 | 68356908 | ID=MS.gene60517.t1.exon4;Parent=MS.gene60517.t1 |
| chr1.2 | CDS | 68356908 | 68357040 | 68356908 | ID=cds.MS.gene60517.t1;Parent=MS.gene60517.t1 |
| chr1.2 | exon | 68359315 | 68359433 | 68359315 | ID=MS.gene60517.t1.exon5;Parent=MS.gene60517.t1 |
| chr1.2 | CDS | 68359315 | 68359433 | 68359315 | ID=cds.MS.gene60517.t1;Parent=MS.gene60517.t1 |
| chr1.2 | exon | 68359820 | 68359887 | 68359820 | ID=MS.gene60517.t1.exon6;Parent=MS.gene60517.t1 |
| chr1.2 | CDS | 68359820 | 68359887 | 68359820 | ID=cds.MS.gene60517.t1;Parent=MS.gene60517.t1 |
| chr1.2 | exon | 68361266 | 68361314 | 68361266 | ID=MS.gene60517.t1.exon7;Parent=MS.gene60517.t1 |
| chr1.2 | CDS | 68361266 | 68361314 | 68361266 | ID=cds.MS.gene60517.t1;Parent=MS.gene60517.t1 |
| chr1.2 | exon | 68361791 | 68362819 | 68361791 | ID=MS.gene60517.t1.exon8;Parent=MS.gene60517.t1 |
| chr1.2 | CDS | 68361791 | 68362819 | 68361791 | ID=cds.MS.gene60517.t1;Parent=MS.gene60517.t1 |
| Gene Sequence |
| Protein sequence |
