AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023270.t1 MTR_5g019460 44.218 294 148 6 47 329 77 365 2.73e-78 244
MS.gene023270.t1 MTR_8g078070 41.695 295 152 8 48 329 79 366 5.98e-74 233
MS.gene023270.t1 MTR_5g092910 31.674 221 133 4 53 263 46 258 3.29e-24 100
MS.gene023270.t1 MTR_2g072470 25.116 215 144 6 61 268 113 317 4.95e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023270.t1 AT4G38460 64.605 291 93 2 45 335 42 322 1.24e-130 376
MS.gene023270.t1 AT4G36810 44.554 303 148 7 42 329 68 365 7.46e-82 253
MS.gene023270.t1 AT2G18620 39.093 353 187 9 1 333 1 345 3.52e-81 251
MS.gene023270.t1 AT2G18640 43.333 300 150 10 48 334 79 371 2.21e-71 226
MS.gene023270.t1 AT2G23800 40.667 300 158 10 48 334 83 375 2.05e-66 214
MS.gene023270.t1 AT3G20160 39.677 310 160 9 43 333 41 342 4.05e-66 212
MS.gene023270.t1 AT3G14550 42.424 297 150 7 48 329 64 354 5.62e-64 207
MS.gene023270.t1 AT3G14550 42.424 297 150 7 48 329 64 354 5.62e-64 207
MS.gene023270.t1 AT3G14550 42.424 297 150 7 48 329 64 354 5.62e-64 207
MS.gene023270.t1 AT3G20160 39.535 301 145 9 43 333 41 314 1.36e-63 204
MS.gene023270.t1 AT3G14530 42.761 297 149 7 48 329 64 354 6.84e-63 204
MS.gene023270.t1 AT3G14510 42.599 277 138 7 68 329 8 278 1.28e-62 201
MS.gene023270.t1 AT3G29430 41.231 325 166 11 24 329 33 351 9.48e-62 201
MS.gene023270.t1 AT3G32040 42.712 295 152 7 48 329 64 354 5.46e-61 199
MS.gene023270.t1 AT1G49530 39.672 305 157 8 52 333 34 334 7.73e-60 196
MS.gene023270.t1 AT1G17050 26.490 151 102 3 119 268 171 313 1.18e-11 65.9

Find 118 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGTTATGTAGAGGTTTA+TGG 0.097267 4.3:-17482094 MS.gene023270:CDS
TTGGATGGATTTGAGAAGTA+TGG 0.258489 4.3:-17482016 MS.gene023270:CDS
AGCAAAGGCTTTGGAGGAAT+TGG 0.259488 4.3:-17482035 MS.gene023270:CDS
CGGTGTTGACATGGCAATTC+TGG 0.263672 4.3:-17482491 MS.gene023270:CDS
TGCTATTGTAGATGATATTT+TGG 0.266530 4.3:-17482164 MS.gene023270:CDS
TGTATCAATCCAACTGCATT+TGG 0.293189 4.3:+17482307 None:intergenic
TCTGATTAATCTGCCCCATT+AGG 0.299471 4.3:+17483366 None:intergenic
GGCTCCACTGGAATGGCTGC+AGG 0.305044 4.3:-17482355 MS.gene023270:CDS
AATGGCTGCAGGGCAGTTCT+TGG 0.308107 4.3:-17482344 MS.gene023270:CDS
AGAGTTATGTAGAGGTTTAT+GGG 0.313757 4.3:-17482093 MS.gene023270:CDS
GGCTGCTCCTTCTCCTTCTT+CGG 0.326678 4.3:-17483441 MS.gene023270:CDS
GGCATTGTCGCAATTGTAAA+AGG 0.335180 4.3:+17483527 None:intergenic
GGGCAGTTCTTGGACCTTGA+AGG 0.335310 4.3:-17482334 MS.gene023270:CDS
CGTAATTCACAAAACTATAG+AGG 0.352365 4.3:+17481978 None:intergenic
GGCAGTTCTTGGACCTTGAA+GGG 0.352960 4.3:-17482333 MS.gene023270:CDS
CTGATTAATCTGCCCCATTA+GGG 0.371204 4.3:+17483367 None:intergenic
TGCCATGTCAACACCGTAGA+TGG 0.385514 4.3:+17482498 None:intergenic
TTTCCCATCTTTATCTCCCT+CGG 0.385637 4.3:+17482126 None:intergenic
CCGTAGATGGCGTGGTTTGA+GGG 0.385791 4.3:+17482511 None:intergenic
CAAGCAGAGATACACATAAC+AGG 0.399894 4.3:+17483251 None:intergenic
GACGTATTGGACTTCCCTAA+TGG 0.407597 4.3:-17483381 MS.gene023270:CDS
ACTTTGATTTGAAGACGTAT+TGG 0.408292 4.3:-17483394 MS.gene023270:CDS
ACACGAAGTACACGGGGTTC+AGG 0.414466 4.3:+17482403 None:intergenic
GTATCAATCCAACTGCATTT+GGG 0.418171 4.3:+17482308 None:intergenic
ATAGAGAGACTGAGGAGGTA+TGG 0.421597 4.3:-17482208 MS.gene023270:CDS
AGAACTTAGAGCAAAGGCTT+TGG 0.423144 4.3:-17482044 MS.gene023270:CDS
GCCCGCTGTGTGGGCTCCAC+TGG 0.424889 4.3:-17482367 MS.gene023270:CDS
ACGTATTGGACTTCCCTAAT+GGG 0.427242 4.3:-17483380 MS.gene023270:CDS
GAAAGAAGATTACCATTTCA+AGG 0.432327 4.3:+17483180 None:intergenic
GCAGCCATGGAGCAGCGAAT+TGG 0.434085 4.3:+17483458 None:intergenic
GGTGATGCACTCTTTCCCCT+TGG 0.434286 4.3:-17482466 MS.gene023270:CDS
TGCAGTGTGTGGAGGGTTGT+TGG 0.437777 4.3:-17482251 MS.gene023270:CDS
AGATGGCGTGGTTTGAGGGC+TGG 0.460200 4.3:+17482515 None:intergenic
ACTTAGAGCAAAGGCTTTGG+AGG 0.466634 4.3:-17482041 MS.gene023270:CDS
CGAATTGGAAGGATAAGATT+CGG 0.471835 4.3:+17483473 None:intergenic
TTGAGGGAACTGAACTTCAA+TGG 0.473114 4.3:+17483331 None:intergenic
CTGTGTGGGCTCCACTGGAA+TGG 0.474784 4.3:-17482362 MS.gene023270:CDS
TCTCTCTATCTCATCATCTT+CGG 0.478860 4.3:+17482222 None:intergenic
GCAGTTCTTGGACCTTGAAG+GGG 0.479193 4.3:-17482332 MS.gene023270:CDS
CACTGCATGTGCCCTTGAAA+TGG 0.480852 4.3:-17483192 MS.gene023270:intron
TTCCCATCTTTATCTCCCTC+GGG 0.481004 4.3:+17482127 None:intergenic
CGGTATTCTGCCCTTGCTAA+AGG 0.483034 4.3:-17483290 MS.gene023270:CDS
CAATGCCCTCATTAAACATC+GGG 0.486811 4.3:-17483511 MS.gene023270:CDS
TCCAGTGGAGCCCACACAGC+GGG 0.487936 4.3:+17482366 None:intergenic
TTGACGAGGCCAGTAGATGA+TGG 0.492007 4.3:-17481934 None:intergenic
ACCGTAGATGGCGTGGTTTG+AGG 0.494704 4.3:+17482510 None:intergenic
CCCTCAAACCACGCCATCTA+CGG 0.495109 4.3:-17482511 MS.gene023270:CDS
AGAAGGAGAAGGAGCAGCCA+TGG 0.495191 4.3:+17483445 None:intergenic
AGGAGGTATGGTAGAGCTGT+TGG 0.495677 4.3:-17482196 MS.gene023270:CDS
CGTGGTTTGAGGGCTGGCCA+CGG 0.508134 4.3:+17482521 None:intergenic
CAGTTCTTGGACCTTGAAGG+GGG 0.508920 4.3:-17482331 MS.gene023270:CDS
AAACCCGAGGGAGATAAAGA+TGG 0.509232 4.3:-17482130 MS.gene023270:CDS
CCTTCCAATTCGCTGCTCCA+TGG 0.517982 4.3:-17483462 MS.gene023270:CDS
GTGTGTGGAGGGTTGTTGGC+CGG 0.518361 4.3:-17482247 MS.gene023270:CDS
ATCGCTTAGCACCTTTAGCA+AGG 0.521626 4.3:+17483279 None:intergenic
TATCTCATCATCTTCGGCAC+CGG 0.522001 4.3:+17482228 None:intergenic
CGCCATCTACGGTGTTGACA+TGG 0.522018 4.3:-17482500 MS.gene023270:CDS
ATGATGAGATAGAGAGACTG+AGG 0.525344 4.3:-17482216 MS.gene023270:CDS
TTCAAGAACCCATCATCTAC+TGG 0.527028 4.3:+17481925 None:intergenic
GCTCCACTGGAATGGCTGCA+GGG 0.527665 4.3:-17482354 MS.gene023270:CDS
GTAATTCACAAAACTATAGA+GGG 0.531875 4.3:+17481979 None:intergenic
GGGCTGGCCACGGCGTGACA+AGG 0.532295 4.3:+17482531 None:intergenic
TGACAAGGAGTCATCCATGC+AGG 0.533253 4.3:+17482546 None:intergenic
ATAGCTTCATATATCTGTTG+AGG 0.536580 4.3:+17483314 None:intergenic
CAGAGGGAAGAGTTATGTAG+AGG 0.537549 4.3:-17482101 MS.gene023270:CDS
GATCGGATGGAAACCGAAGA+AGG 0.542003 4.3:+17483428 None:intergenic
GAAACACCCGATGTTTAATG+AGG 0.542457 4.3:+17483505 None:intergenic
GAGTTATGTAGAGGTTTATG+GGG 0.542842 4.3:-17482092 MS.gene023270:CDS
AACAATGTGTTGAAATCCAA+GGG 0.546111 4.3:+17482450 None:intergenic
CATTTCAAGGGCACATGCAG+TGG 0.547793 4.3:+17483193 None:intergenic
TAGCTTCATATATCTGTTGA+GGG 0.547993 4.3:+17483315 None:intergenic
AAGGCTTTGGAGGAATTGGA+TGG 0.548932 4.3:-17482031 MS.gene023270:CDS
GAGTGTTCTGCAGTGTGTGG+AGG 0.552684 4.3:-17482259 MS.gene023270:CDS
AAGGGCACATGCAGTGGGGA+AGG 0.558957 4.3:+17483199 None:intergenic
AAAGAAGATTACCATTTCAA+GGG 0.560180 4.3:+17483181 None:intergenic
GTACACGGGGTTCAGGTACA+AGG 0.565661 4.3:+17482410 None:intergenic
ATCATCATGTATCAATGAAG+CGG 0.568247 4.3:+17482573 None:intergenic
ATGCAGTGGGGAAGGCAGCA+AGG 0.568393 4.3:+17483207 None:intergenic
ATGAGATAGAGAGACTGAGG+AGG 0.573192 4.3:-17482213 MS.gene023270:CDS
AACCCGAGGGAGATAAAGAT+GGG 0.574299 4.3:-17482129 MS.gene023270:CDS
ATTTCAAGGGCACATGCAGT+GGG 0.576737 4.3:+17483194 None:intergenic
CAGCAATGACACGAAGTACA+CGG 0.576879 4.3:+17482395 None:intergenic
AGTGTTCTGCAGTGTGTGGA+GGG 0.579045 4.3:-17482258 MS.gene023270:CDS
GGCTGAAGAACTTAGAGCAA+AGG 0.579804 4.3:-17482050 MS.gene023270:CDS
CTGCCCTGCAGCCATTCCAG+TGG 0.580181 4.3:+17482351 None:intergenic
GCTGAGATAGCCCGCTGTGT+GGG 0.580286 4.3:-17482376 MS.gene023270:CDS
AGCAATGACACGAAGTACAC+GGG 0.582072 4.3:+17482396 None:intergenic
GTTGACATGGCAATTCTGGC+GGG 0.592172 4.3:-17482487 MS.gene023270:CDS
TCGCTTAGCACCTTTAGCAA+GGG 0.604739 4.3:+17483280 None:intergenic
ACATGATGATCTTCCCTGCA+TGG 0.608437 4.3:-17482560 MS.gene023270:CDS
ACAATGCCCTCATTAAACAT+CGG 0.612387 4.3:-17483512 MS.gene023270:CDS
GGGTTCAGGTACAAGGTCAG+AGG 0.613058 4.3:+17482417 None:intergenic
CCATGGAGCAGCGAATTGGA+AGG 0.614799 4.3:+17483462 None:intergenic
ATGGAAACCGAAGAAGGAGA+AGG 0.615829 4.3:+17483434 None:intergenic
TGTTGACATGGCAATTCTGG+CGG 0.616197 4.3:-17482488 MS.gene023270:CDS
AAACAATGTGTTGAAATCCA+AGG 0.617990 4.3:+17482449 None:intergenic
AAGCAGAGATACACATAACA+GGG 0.618552 4.3:+17483252 None:intergenic
GATGACTCCTTGTCACGCCG+TGG 0.624688 4.3:-17482538 MS.gene023270:CDS
GACAAGGAGTCATCCATGCA+GGG 0.630249 4.3:+17482547 None:intergenic
GCGGAGTGTTCTGCAGTGTG+TGG 0.632086 4.3:-17482262 MS.gene023270:CDS
GGAAGAGAGACTGAAACCCG+AGG 0.632492 4.3:-17482143 MS.gene023270:CDS
TGCTGAGATAGCCCGCTGTG+TGG 0.636542 4.3:-17482377 MS.gene023270:CDS
AAACACCCGATGTTTAATGA+GGG 0.640367 4.3:+17483506 None:intergenic
TGTCAACACCGTAGATGGCG+TGG 0.643803 4.3:+17482503 None:intergenic
GCAGAGATACACATAACAGG+GGG 0.650726 4.3:+17483254 None:intergenic
AGAGATACACATAACAGGGG+GGG 0.650807 4.3:+17483256 None:intergenic
TAGAAGTTTCAATGTTGACG+AGG 0.655023 4.3:-17481948 MS.gene023270:CDS
CGTATTGGACTTCCCTAATG+GGG 0.660461 4.3:-17483379 MS.gene023270:CDS
GAAGAGAGACTGAAACCCGA+GGG 0.662153 4.3:-17482142 MS.gene023270:CDS
GGTTCAGGTACAAGGTCAGA+GGG 0.665434 4.3:+17482418 None:intergenic
AACAGATATATGAAGCTATG+CGG 0.703508 4.3:-17483310 MS.gene023270:CDS
TAATTCACAAAACTATAGAG+GGG 0.709745 4.3:+17481980 None:intergenic
AGCAGAGATACACATAACAG+GGG 0.710591 4.3:+17483253 None:intergenic
TTCCAGTGGAGCCCACACAG+CGG 0.722527 4.3:+17482365 None:intergenic
ACAATGTGTTGAAATCCAAG+GGG 0.726426 4.3:+17482451 None:intergenic
CAGAGATACACATAACAGGG+GGG 0.735594 4.3:+17483255 None:intergenic
TTTCAAGGGCACATGCAGTG+GGG 0.742785 4.3:+17483195 None:intergenic
GCAATGACACGAAGTACACG+GGG 0.769728 4.3:+17482397 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGTTTGGTATTTCTTAT+TGG - chr4.3:17482755-17482774 MS.gene023270:intron 20.0%
!! TTGATACAAGATAAAAAGTT+TGG - chr4.3:17483178-17483197 MS.gene023270:intron 20.0%
!!! ATGATAAACTGTATGAATTT+TGG - chr4.3:17482843-17482862 MS.gene023270:intron 20.0%
! AAAGAAGATTACCATTTCAA+GGG + chr4.3:17482292-17482311 None:intergenic 25.0%
! AATATTCAGAGACACATTTA+AGG + chr4.3:17482736-17482755 None:intergenic 25.0%
! AATCAAAATTGGAAAGAGAA+AGG + chr4.3:17482520-17482539 None:intergenic 25.0%
! ACAATAGAATGAATATGAAG+TGG + chr4.3:17482325-17482344 None:intergenic 25.0%
! GATAGGTAAAAGTAAAACTT+TGG + chr4.3:17482437-17482456 None:intergenic 25.0%
! GTAATTCACAAAACTATAGA+GGG + chr4.3:17483494-17483513 None:intergenic 25.0%
! TAATTCACAAAACTATAGAG+GGG + chr4.3:17483493-17483512 None:intergenic 25.0%
! TGTCTCTGAATATTTATGTT+TGG - chr4.3:17482742-17482761 MS.gene023270:intron 25.0%
!! AGATAAAAAGTTTGGTGAAA+TGG - chr4.3:17483186-17483205 MS.gene023270:intron 25.0%
!! TGCTATTGTAGATGATATTT+TGG - chr4.3:17483306-17483325 MS.gene023270:CDS 25.0%
!!! ATAGTACATACTTTGACTTT+TGG - chr4.3:17482681-17482700 MS.gene023270:intron 25.0%
AAACAATGTGTTGAAATCCA+AGG + chr4.3:17483024-17483043 None:intergenic 30.0%
AACAATGTGTTGAAATCCAA+GGG + chr4.3:17483023-17483042 None:intergenic 30.0%
AACAGATATATGAAGCTATG+CGG - chr4.3:17482160-17482179 MS.gene023270:CDS 30.0%
AAGAGTTATGTAGAGGTTTA+TGG - chr4.3:17483376-17483395 MS.gene023270:CDS 30.0%
AGAGTTATGTAGAGGTTTAT+GGG - chr4.3:17483377-17483396 MS.gene023270:CDS 30.0%
ATAAAGATGGGAAAAACAGA+GGG - chr4.3:17483353-17483372 MS.gene023270:CDS 30.0%
ATAGCTTCATATATCTGTTG+AGG + chr4.3:17482159-17482178 None:intergenic 30.0%
ATCATCATGTATCAATGAAG+CGG + chr4.3:17482900-17482919 None:intergenic 30.0%
CAGATACAACTAGCTAAAAA+TGG + chr4.3:17482655-17482674 None:intergenic 30.0%
CGTAATTCACAAAACTATAG+AGG + chr4.3:17483495-17483514 None:intergenic 30.0%
GAAAGAAGATTACCATTTCA+AGG + chr4.3:17482293-17482312 None:intergenic 30.0%
TAGCTTCATATATCTGTTGA+GGG + chr4.3:17482158-17482177 None:intergenic 30.0%
! TAAAAAGTTTGGTGAAATGG+CGG - chr4.3:17483189-17483208 MS.gene023270:intron 30.0%
! TAGGAACGGATTTATTTGAT+AGG + chr4.3:17482454-17482473 None:intergenic 30.0%
!! ACTTTGATTTGAAGACGTAT+TGG - chr4.3:17482076-17482095 MS.gene023270:CDS 30.0%
!!! CTTATATTTTAGTTGCAAGC+AGG - chr4.3:17482868-17482887 MS.gene023270:intron 30.0%
AAACACCCGATGTTTAATGA+GGG + chr4.3:17481967-17481986 None:intergenic 35.0%
AAGCAGAGATACACATAACA+GGG + chr4.3:17482221-17482240 None:intergenic 35.0%
ACAATGCCCTCATTAAACAT+CGG - chr4.3:17481958-17481977 MS.gene023270:CDS 35.0%
ACAATGTGTTGAAATCCAAG+GGG + chr4.3:17483022-17483041 None:intergenic 35.0%
CGAATTGGAAGGATAAGATT+CGG + chr4.3:17482000-17482019 None:intergenic 35.0%
GAGTTATGTAGAGGTTTATG+GGG - chr4.3:17483378-17483397 MS.gene023270:CDS 35.0%
GATAAAGATGGGAAAAACAG+AGG - chr4.3:17483352-17483371 MS.gene023270:CDS 35.0%
GTATCAATCCAACTGCATTT+GGG + chr4.3:17483165-17483184 None:intergenic 35.0%
TAGAAGTTTCAATGTTGACG+AGG - chr4.3:17483522-17483541 MS.gene023270:CDS 35.0%
TCTCTCTATCTCATCATCTT+CGG + chr4.3:17483251-17483270 None:intergenic 35.0%
TGTATCAATCCAACTGCATT+TGG + chr4.3:17483166-17483185 None:intergenic 35.0%
! ATCTCTGCTTGTGAACTTTT+CGG - chr4.3:17482231-17482250 MS.gene023270:CDS 35.0%
! CAAAGTGAACAGCTTTTGAT+CGG + chr4.3:17482062-17482081 None:intergenic 35.0%
! GGATAAGATTCGGAGATTTT+CGG + chr4.3:17481990-17482009 None:intergenic 35.0%
! GGATGAGCTTTCTTTTTCTT+TGG - chr4.3:17482490-17482509 MS.gene023270:CDS 35.0%
! TTGGATGGATTTGAGAAGTA+TGG - chr4.3:17483454-17483473 MS.gene023270:CDS 35.0%
AAAGAAAGCTCATCCTCTCA+CGG + chr4.3:17482485-17482504 None:intergenic 40.0%
ACGTATTGGACTTCCCTAAT+GGG - chr4.3:17482090-17482109 MS.gene023270:CDS 40.0%
AGAACTTAGAGCAAAGGCTT+TGG - chr4.3:17483426-17483445 MS.gene023270:CDS 40.0%
AGCAGAGATACACATAACAG+GGG + chr4.3:17482220-17482239 None:intergenic 40.0%
ATGATGAGATAGAGAGACTG+AGG - chr4.3:17483254-17483273 MS.gene023270:CDS 40.0%
CAAGCAGAGATACACATAAC+AGG + chr4.3:17482222-17482241 None:intergenic 40.0%
CAATGCCCTCATTAAACATC+GGG - chr4.3:17481959-17481978 MS.gene023270:CDS 40.0%
CTGATTAATCTGCCCCATTA+GGG + chr4.3:17482106-17482125 None:intergenic 40.0%
GAAACACCCGATGTTTAATG+AGG + chr4.3:17481968-17481987 None:intergenic 40.0%
GACAATGATAGACCAATCTC+TGG + chr4.3:17482385-17482404 None:intergenic 40.0%
GGCATTGTCGCAATTGTAAA+AGG + chr4.3:17481946-17481965 None:intergenic 40.0%
TCTGATTAATCTGCCCCATT+AGG + chr4.3:17482107-17482126 None:intergenic 40.0%
TTGAGGGAACTGAACTTCAA+TGG + chr4.3:17482142-17482161 None:intergenic 40.0%
TTTCCCATCTTTATCTCCCT+CGG + chr4.3:17483347-17483366 None:intergenic 40.0%
AAACCCGAGGGAGATAAAGA+TGG - chr4.3:17483340-17483359 MS.gene023270:CDS 45.0%
AACCCGAGGGAGATAAAGAT+GGG - chr4.3:17483341-17483360 MS.gene023270:CDS 45.0%
ACATGATGATCTTCCCTGCA+TGG - chr4.3:17482910-17482929 MS.gene023270:intron 45.0%
AGAGATACACATAACAGGGG+GGG + chr4.3:17482217-17482236 None:intergenic 45.0%
AGCAATGACACGAAGTACAC+GGG + chr4.3:17483077-17483096 None:intergenic 45.0%
ATAGAGAGACTGAGGAGGTA+TGG - chr4.3:17483262-17483281 MS.gene023270:CDS 45.0%
ATCGCTTAGCACCTTTAGCA+AGG + chr4.3:17482194-17482213 None:intergenic 45.0%
ATGAGATAGAGAGACTGAGG+AGG - chr4.3:17483257-17483276 MS.gene023270:CDS 45.0%
ATGGAAACCGAAGAAGGAGA+AGG + chr4.3:17482039-17482058 None:intergenic 45.0%
CAGAGATACACATAACAGGG+GGG + chr4.3:17482218-17482237 None:intergenic 45.0%
CAGAGGGAAGAGTTATGTAG+AGG - chr4.3:17483369-17483388 MS.gene023270:CDS 45.0%
CAGCAATGACACGAAGTACA+CGG + chr4.3:17483078-17483097 None:intergenic 45.0%
CGTATTGGACTTCCCTAATG+GGG - chr4.3:17482091-17482110 MS.gene023270:CDS 45.0%
GACGTATTGGACTTCCCTAA+TGG - chr4.3:17482089-17482108 MS.gene023270:CDS 45.0%
GCAGAGATACACATAACAGG+GGG + chr4.3:17482219-17482238 None:intergenic 45.0%
GGCTGAAGAACTTAGAGCAA+AGG - chr4.3:17483420-17483439 MS.gene023270:CDS 45.0%
TATCTCATCATCTTCGGCAC+CGG + chr4.3:17483245-17483264 None:intergenic 45.0%
TCGCTTAGCACCTTTAGCAA+GGG + chr4.3:17482193-17482212 None:intergenic 45.0%
TGTTGACATGGCAATTCTGG+CGG - chr4.3:17482982-17483001 MS.gene023270:intron 45.0%
TTCCCATCTTTATCTCCCTC+GGG + chr4.3:17483346-17483365 None:intergenic 45.0%
! ACTTAGAGCAAAGGCTTTGG+AGG - chr4.3:17483429-17483448 MS.gene023270:CDS 45.0%
! ATTTCAAGGGCACATGCAGT+GGG + chr4.3:17482279-17482298 None:intergenic 45.0%
! GTGAACAGCTTTTGATCGGA+TGG + chr4.3:17482058-17482077 None:intergenic 45.0%
! TCTGCTTGTGAACTTTTCGG+AGG - chr4.3:17482234-17482253 MS.gene023270:CDS 45.0%
!! AAGGCTTTGGAGGAATTGGA+TGG - chr4.3:17483439-17483458 MS.gene023270:CDS 45.0%
!! AGCAAAGGCTTTGGAGGAAT+TGG - chr4.3:17483435-17483454 MS.gene023270:CDS 45.0%
!!! TTATTTTAATAAATCAAAAT+TGG + chr4.3:17482531-17482550 None:intergenic 5.0%
AAAGCTCATCCTCTCACGGT+AGG + chr4.3:17482481-17482500 None:intergenic 50.0%
AGTGTTCTGCAGTGTGTGGA+GGG - chr4.3:17483212-17483231 MS.gene023270:CDS 50.0%
CACTGCATGTGCCCTTGAAA+TGG - chr4.3:17482278-17482297 MS.gene023270:CDS 50.0%
CAGTTCTTGGACCTTGAAGG+GGG - chr4.3:17483139-17483158 MS.gene023270:intron 50.0%
CGGTATTCTGCCCTTGCTAA+AGG - chr4.3:17482180-17482199 MS.gene023270:CDS 50.0%
GAAGAGAGACTGAAACCCGA+GGG - chr4.3:17483328-17483347 MS.gene023270:CDS 50.0%
GACAAGGAGTCATCCATGCA+GGG + chr4.3:17482926-17482945 None:intergenic 50.0%
GATCGGATGGAAACCGAAGA+AGG + chr4.3:17482045-17482064 None:intergenic 50.0%
GCAATGACACGAAGTACACG+GGG + chr4.3:17483076-17483095 None:intergenic 50.0%
GCAGTTCTTGGACCTTGAAG+GGG - chr4.3:17483138-17483157 MS.gene023270:intron 50.0%
GCTAAGAACTCGCCAGAGAT+TGG - chr4.3:17482370-17482389 MS.gene023270:CDS 50.0%
GGCAGTTCTTGGACCTTGAA+GGG - chr4.3:17483137-17483156 MS.gene023270:intron 50.0%
GGTTCAGGTACAAGGTCAGA+GGG + chr4.3:17483055-17483074 None:intergenic 50.0%
GGTTGAAAAAGCAGCAGAGG+TGG - chr4.3:17483399-17483418 MS.gene023270:CDS 50.0%
GTTGACATGGCAATTCTGGC+GGG - chr4.3:17482983-17483002 MS.gene023270:intron 50.0%
TCACGGTAGGCAGTTAGGAA+CGG + chr4.3:17482468-17482487 None:intergenic 50.0%
TGACAAGGAGTCATCCATGC+AGG + chr4.3:17482927-17482946 None:intergenic 50.0%
TGCCATGTCAACACCGTAGA+TGG + chr4.3:17482975-17482994 None:intergenic 50.0%
TGGGGTTGAAAAAGCAGCAG+AGG - chr4.3:17483396-17483415 MS.gene023270:CDS 50.0%
! AGGAGGTATGGTAGAGCTGT+TGG - chr4.3:17483274-17483293 MS.gene023270:CDS 50.0%
! CATTTCAAGGGCACATGCAG+TGG + chr4.3:17482280-17482299 None:intergenic 50.0%
! TTTCAAGGGCACATGCAGTG+GGG + chr4.3:17482278-17482297 None:intergenic 50.0%
!! CGGTGTTGACATGGCAATTC+TGG - chr4.3:17482979-17482998 MS.gene023270:intron 50.0%
AATGGCTGCAGGGCAGTTCT+TGG - chr4.3:17483126-17483145 MS.gene023270:intron 55.0%
ACACGAAGTACACGGGGTTC+AGG + chr4.3:17483070-17483089 None:intergenic 55.0%
ACCGTAGATGGCGTGGTTTG+AGG + chr4.3:17482963-17482982 None:intergenic 55.0%
AGAAGGAGAAGGAGCAGCCA+TGG + chr4.3:17482028-17482047 None:intergenic 55.0%
CCATGGAGCAGCGAATTGGA+AGG + chr4.3:17482011-17482030 None:intergenic 55.0%
CCCTCAAACCACGCCATCTA+CGG - chr4.3:17482959-17482978 MS.gene023270:intron 55.0%
CCGTAGATGGCGTGGTTTGA+GGG + chr4.3:17482962-17482981 None:intergenic 55.0%
CCTTCCAATTCGCTGCTCCA+TGG - chr4.3:17482008-17482027 MS.gene023270:CDS 55.0%
GAGTGTTCTGCAGTGTGTGG+AGG - chr4.3:17483211-17483230 MS.gene023270:CDS 55.0%
GGAAGAGAGACTGAAACCCG+AGG - chr4.3:17483327-17483346 MS.gene023270:CDS 55.0%
GGCTGCTCCTTCTCCTTCTT+CGG - chr4.3:17482029-17482048 MS.gene023270:CDS 55.0%
GGGCAGTTCTTGGACCTTGA+AGG - chr4.3:17483136-17483155 MS.gene023270:intron 55.0%
GGGTTCAGGTACAAGGTCAG+AGG + chr4.3:17483056-17483075 None:intergenic 55.0%
GGTGATGCACTCTTTCCCCT+TGG - chr4.3:17483004-17483023 MS.gene023270:intron 55.0%
GTACACGGGGTTCAGGTACA+AGG + chr4.3:17483063-17483082 None:intergenic 55.0%
TCCTAACTGCCTACCGTGAG+AGG - chr4.3:17482469-17482488 MS.gene023270:CDS 55.0%
TCCTCTCACGGTAGGCAGTT+AGG + chr4.3:17482473-17482492 None:intergenic 55.0%
TGCAGTGTGTGGAGGGTTGT+TGG - chr4.3:17483219-17483238 MS.gene023270:CDS 55.0%
TGTCAACACCGTAGATGGCG+TGG + chr4.3:17482970-17482989 None:intergenic 55.0%
!! CGCCATCTACGGTGTTGACA+TGG - chr4.3:17482970-17482989 MS.gene023270:intron 55.0%
AGATGGCGTGGTTTGAGGGC+TGG + chr4.3:17482958-17482977 None:intergenic 60.0%
ATGCAGTGGGGAAGGCAGCA+AGG + chr4.3:17482266-17482285 None:intergenic 60.0%
CTGCATTTGGGCCCCCTTCA+AGG + chr4.3:17483153-17483172 None:intergenic 60.0%
CTGTGTGGGCTCCACTGGAA+TGG - chr4.3:17483108-17483127 MS.gene023270:intron 60.0%
GAAGGGGGCCCAAATGCAGT+TGG - chr4.3:17483154-17483173 MS.gene023270:intron 60.0%
GATGACTCCTTGTCACGCCG+TGG - chr4.3:17482932-17482951 MS.gene023270:intron 60.0%
GCAGCCATGGAGCAGCGAAT+TGG + chr4.3:17482015-17482034 None:intergenic 60.0%
GCTCCACTGGAATGGCTGCA+GGG - chr4.3:17483116-17483135 MS.gene023270:intron 60.0%
GCTGAGATAGCCCGCTGTGT+GGG - chr4.3:17483094-17483113 MS.gene023270:intron 60.0%
GTGTGTGGAGGGTTGTTGGC+CGG - chr4.3:17483223-17483242 MS.gene023270:CDS 60.0%
TGCTGAGATAGCCCGCTGTG+TGG - chr4.3:17483093-17483112 MS.gene023270:intron 60.0%
TTCCAGTGGAGCCCACACAG+CGG + chr4.3:17483108-17483127 None:intergenic 60.0%
! AAGGGCACATGCAGTGGGGA+AGG + chr4.3:17482274-17482293 None:intergenic 60.0%
! GCGGAGTGTTCTGCAGTGTG+TGG - chr4.3:17483208-17483227 MS.gene023270:CDS 60.0%
CTGCCCTGCAGCCATTCCAG+TGG + chr4.3:17483122-17483141 None:intergenic 65.0%
GGCTCCACTGGAATGGCTGC+AGG - chr4.3:17483115-17483134 MS.gene023270:intron 65.0%
TCCAGTGGAGCCCACACAGC+GGG + chr4.3:17483107-17483126 None:intergenic 65.0%
!! CGTGGTTTGAGGGCTGGCCA+CGG + chr4.3:17482952-17482971 None:intergenic 65.0%
GCCCGCTGTGTGGGCTCCAC+TGG - chr4.3:17483103-17483122 MS.gene023270:intron 75.0%
!! GGGCTGGCCACGGCGTGACA+AGG + chr4.3:17482942-17482961 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 17481937 17483555 17481937 ID=MS.gene023270
chr4.3 mRNA 17481937 17483555 17481937 ID=MS.gene023270.t1;Parent=MS.gene023270
chr4.3 exon 17483193 17483555 17483193 ID=MS.gene023270.t1.exon1;Parent=MS.gene023270.t1
chr4.3 CDS 17483193 17483555 17483193 ID=cds.MS.gene023270.t1;Parent=MS.gene023270.t1
chr4.3 exon 17481937 17482602 17481937 ID=MS.gene023270.t1.exon2;Parent=MS.gene023270.t1
chr4.3 CDS 17481937 17482602 17481937 ID=cds.MS.gene023270.t1;Parent=MS.gene023270.t1
Gene Sequence

>MS.gene023270

ATGGCTCCTTTTACAATTGCGACAATGCCCTCATTAAACATCGGGTGTTTCCGAAAATCTCCGAATCTTATCCTTCCAATTCGCTGCTCCATGGCTGCTCCTTCTCCTTCTTCGGTTTCCATCCGATCAAAAGCTGTTCACTTTGATTTGAAGACGTATTGGACTTCCCTAATGGGGCAGATTAATCAGAAGCTCGATGAAGCCATTGAAGTTCAGTTCCCTCAACAGATATATGAAGCTATGCGGTATTCTGCCCTTGCTAAAGGTGCTAAGCGATCCCCCCCTGTTATGTGTATCTCTGCTTGTGAACTTTTCGGAGGCAGTCGCCTTGCTGCCTTCCCCACTGCATGTGCCCTTGAAATGGTAATCTTCTTTCTATAATCTGCCACTTCATATTCATTCTATTGTATTATGTTTTTAGTTTGTTTTTTATGCTAAGAACTCGCCAGAGATTGGTCTATCATTGTCACAAATTTATTTGTTATGTTGTTGAGCTACCAAAGTTTTACTTTTACCTATCAAATAAATCCGTTCCTAACTGCCTACCGTGAGAGGATGAGCTTTCTTTTTCTTTGGACTTCCTTTCTCTTTCCAATTTTGATTTATTAAAATAATAACATATGTTGCATATTTCTTCAAAAAAAAAAAAAAAACATATGTTGCATGTTTAGTTGCAGTAATGCTGTGTTGCTCATATAAGTAACAGTTTTGCATTCCATTTTTAGCTAGTTGTATCTGCTTAAAATAGTACATACTTTGACTTTTGGATCATCTGTTAAGAATGATTGTAGCATTACCTTAAATGTGTCTCTGAATATTTATGTTTGGTATTTCTTATTGGACACAAAGTGAGATTTATGAAGCGTTTATATAATTATATGCACTTCAGATAAATAAATAATGATGATGATAAACTGTATGAATTTTGGAACTTATATTTTAGTTGCAAGCAGGTACATGCCGCTTCATTGATACATGATGATCTTCCCTGCATGGATGACTCCTTGTCACGCCGTGGCCAGCCCTCAAACCACGCCATCTACGGTGTTGACATGGCAATTCTGGCGGGTGATGCACTCTTTCCCCTTGGATTTCAACACATTGTTTCACATACTCCCTCTGACCTTGTACCTGAACCCCGTGTACTTCGTGTCATTGCTGAGATAGCCCGCTGTGTGGGCTCCACTGGAATGGCTGCAGGGCAGTTCTTGGACCTTGAAGGGGGCCCAAATGCAGTTGGATTGATACAAGATAAAAAGTTTGGTGAAATGGCGGAGTGTTCTGCAGTGTGTGGAGGGTTGTTGGCCGGTGCCGAAGATGATGAGATAGAGAGACTGAGGAGGTATGGTAGAGCTGTTGGAGTATTGTATGCTATTGTAGATGATATTTTGGAAGAGAGACTGAAACCCGAGGGAGATAAAGATGGGAAAAACAGAGGGAAGAGTTATGTAGAGGTTTATGGGGTTGAAAAAGCAGCAGAGGTGGCTGAAGAACTTAGAGCAAAGGCTTTGGAGGAATTGGATGGATTTGAGAAGTATGGAGATCAAGTGTTGCCCCTCTATAGTTTTGTGAATTACGCTATTGATAGAAGTTTCAATGTTGACGAGGCCAGTAGATGA
Protein sequence

>MS.gene023270.t1

MAPFTIATMPSLNIGCFRKSPNLILPIRCSMAAPSPSSVSIRSKAVHFDLKTYWTSLMGQINQKLDEAIEVQFPQQIYEAMRYSALAKGAKRSPPVMCISACELFGGSRLAAFPTACALEMVHAASLIHDDLPCMDDSLSRRGQPSNHAIYGVDMAILAGDALFPLGFQHIVSHTPSDLVPEPRVLRVIAEIARCVGSTGMAAGQFLDLEGGPNAVGLIQDKKFGEMAECSAVCGGLLAGAEDDEIERLRRYGRAVGVLYAIVDDILEERLKPEGDKDGKNRGKSYVEVYGVEKAAEVAEELRAKALEELDGFEKYGDQVLPLYSFVNYAIDRSFNVDEASR