AlfalfaGEDB | MS.gene02393

M. sativa cultivar XinJiangDaYe / MS.gene02393

KEGG: K02115
GO: GO:0003674 GO:0003824 GO:0005215 GO:0005216 GO:0005261 GO:0005575 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009165 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0015075 GO:0015077 GO:0015078 GO:0015252 GO:0015267 GO:0015318 GO:0015672 GO:0015985 GO:0015986 GO:0016740 GO:0016772 GO:0016776 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0022803 GO:0022857 GO:0022890 GO:0032991 GO:0033178 GO:0034220 GO:0034641 GO:0034654 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0045261 GO:0046034 GO:0046390 GO:0046483 GO:0046933 GO:0051179 GO:0051234 GO:0055085 GO:0055086 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
PFAM: PF00231.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02393.t1	ACJ84643.1	93	374	26	0	1	374	1	374	1.60E-185	658.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02393.t1	P28552	91.9	369	30	0	1	369	1	369	4.2e-174	612.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02393.t1	B7FIR4	93.0	374	26	0	1	374	1	374	1.2e-185	658.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene02393	MS.gene57960	PPI
MS.gene035640	MS.gene02393	PPI
MS.gene02393	MS.gene055477	PPI
MS.gene02393	MS.gene012472	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02393.t1	MTR_2g023290	97.952	293	6	0	82	374	729	1021	0.0	587
MS.gene02393.t1	MTR_3g052270	68.155	336	101	2	44	373	12	347	4.26e-168	473
MS.gene02393.t1	MTR_1588s0010	99.010	101	1	0	1	101	1	101	2.09e-65	203
MS.gene02393.t1	MTR_4g129790	29.301	372	193	13	9	373	17	325	1.86e-26	108

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02393.t1	AT4G04640	81.117	376	66	3	1	374	1	373	0.0	599
MS.gene02393.t1	AT1G15700	70.725	345	100	1	30	373	39	383	2.38e-177	498
MS.gene02393.t1	AT2G33040	31.790	324	164	12	57	373	48	321	7.70e-27	109

Find 61 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTTAGACCACACTGAATT+TGG	0.110416	2.2:+62357319	None:intergenic
TATTATCAGTGTTGGTAAAA+AGG	0.215367	2.2:-62356965	MS.gene02393:CDS
GGTGAAATCTGTGATATTAA+TGG	0.242769	2.2:-62356724	MS.gene02393:CDS
GCTCAAGAAGCTGTTGTTAA+TGG	0.249925	2.2:-62357210	MS.gene02393:CDS
ATTGCTGAGTTGAAAGTTCT+TGG	0.268699	2.2:-62357000	MS.gene02393:CDS
TCAAATCTTGAGGGCTTTGC+AGG	0.276662	2.2:-62356527	MS.gene02393:CDS
GTGACAGTGGAAGCAAAGTA+TGG	0.339355	2.2:+62356752	None:intergenic
GGAAGCAAAGTATGGATTAT+AGG	0.339933	2.2:+62356760	None:intergenic
ATATCACAGATTTCACCTTT+TGG	0.359402	2.2:+62356730	None:intergenic
AACCTATCAACAGGTATATA+AGG	0.362640	2.2:+62356919	None:intergenic
TCCTGCTCAAACTGCAAAAT+CGG	0.366440	2.2:+62356595	None:intergenic
TCTTAGACCACACTGAATTT+GGG	0.374759	2.2:+62357320	None:intergenic
ACTGGTCTAAGCTTAGTTAG+AGG	0.375333	2.2:+62357111	None:intergenic
CAGCACCAGCAACAATTTCC+AGG	0.387882	2.2:+62356374	None:intergenic
TGAATTTGGGTTGGTCTTGA+AGG	0.393465	2.2:+62357333	None:intergenic
GACCTTATATACCTGTTGAT+AGG	0.399954	2.2:-62356921	MS.gene02393:CDS
CTGTTGAAAGAGATGCTGTT+AGG	0.407775	2.2:-62356639	MS.gene02393:CDS
TGCATCAGGTAATGAAGGTT+TGG	0.412967	2.2:+62357428	None:intergenic
CCTGTTGATAGGTTTCTTGA+AGG	0.420186	2.2:-62356910	MS.gene02393:CDS
TTCAGGTTAACAACGAAAGA+AGG	0.449455	2.2:-62356670	MS.gene02393:CDS
TTTGAACAGTCAAATCTTGA+GGG	0.462626	2.2:-62356536	MS.gene02393:CDS
GTTGCTCTTGTTGTTTGCAC+TGG	0.463249	2.2:-62357081	MS.gene02393:CDS
TGTTGCTGGTGCTGATGCCT+TGG	0.464852	2.2:-62356365	MS.gene02393:CDS
CCTTCAAGAAACCTATCAAC+AGG	0.466272	2.2:+62356910	None:intergenic
TCAACTAGTGTCTGTGAGAA+AGG	0.467803	2.2:+62357183	None:intergenic
ATTTGACTGTTCAAATAAAG+TGG	0.475054	2.2:+62356544	None:intergenic
TAAGATCACTGGTGAAATCC+TGG	0.483893	2.2:-62356392	MS.gene02393:CDS
AGTGCATCAAGAATCTGAAC+AGG	0.485687	2.2:+62356571	None:intergenic
GAAATCCTGGAAATTGTTGC+TGG	0.495138	2.2:-62356379	MS.gene02393:CDS
AAGGAGAGGTTAGATGCATC+AGG	0.495737	2.2:+62357414	None:intergenic
GTTGTTTGCACTGGTGATAG+AGG	0.498449	2.2:-62357072	MS.gene02393:CDS
GTGCTTCTTTAGCAGTAGGA+AGG	0.507770	2.2:+62356881	None:intergenic
TTGCTTCCACTGTCACCAAA+AGG	0.509640	2.2:-62356745	MS.gene02393:CDS
TTAGATGCATCAGGTAATGA+AGG	0.514624	2.2:+62357423	None:intergenic
GTTGATAGGTTTCTTGAAGG+TGG	0.518256	2.2:-62356907	MS.gene02393:CDS
TTTCACCAGTGATCTTAGCT+TGG	0.530031	2.2:+62356398	None:intergenic
GTTTGTGCTTCTTTAGCAGT+AGG	0.537662	2.2:+62356877	None:intergenic
ATTTGAACAGTCAAATCTTG+AGG	0.540431	2.2:-62356537	MS.gene02393:CDS
CAACTTCTTCACTAACAAAG+AGG	0.544704	2.2:+62356833	None:intergenic
GATTACACTATTATCAGTGT+TGG	0.547808	2.2:-62356973	MS.gene02393:CDS
ATCAGGTAATGAAGGTTTGG+AGG	0.549600	2.2:+62357431	None:intergenic
GCTGTTCGTTGATGCTGTAA+AGG	0.551485	2.2:+62357157	None:intergenic
TTTGTGAAGGAAGCTGAAAA+GGG	0.553199	2.2:+62357376	None:intergenic
AGACCACACTGAATTTGGGT+TGG	0.556222	2.2:+62357324	None:intergenic
GTTGAAGAAGAATTTGTCAA+GGG	0.556839	2.2:-62356437	MS.gene02393:CDS
AGGGCTGAGAGCTGAACGGA+AGG	0.577278	2.2:+62357395	None:intergenic
AGTTGAAGAAGAATTTGTCA+AGG	0.578085	2.2:-62356438	MS.gene02393:CDS
CAACGCCAAGCTAAGATCAC+TGG	0.583862	2.2:-62356403	MS.gene02393:CDS
TAGAGGAGCTTCGATATCGT+CGG	0.592502	2.2:+62357128	None:intergenic
AACTTCTTCACTAACAAAGA+GGG	0.594771	2.2:+62356834	None:intergenic
GGTGATAGAGGTCTCTGTGG+TGG	0.597310	2.2:-62357060	MS.gene02393:CDS
CACTGAAGCAATGAAGCTTG+TGG	0.611051	2.2:-62357253	MS.gene02393:CDS
TTGCTCAAATATCACCATGT+TGG	0.614531	2.2:-62357457	MS.gene02393:CDS
TGAGAGCTGAACGGAAGGAG+AGG	0.630596	2.2:+62357400	None:intergenic
AAACTGTGTTGATGCTGCTG+AGG	0.639433	2.2:-62356701	MS.gene02393:CDS
GAAAAGGGCTGAGAGCTGAA+CGG	0.641643	2.2:+62357391	None:intergenic
TATAAATACACTCTATACCA+AGG	0.641733	2.2:+62356348	None:intergenic
CAGGTATATAAGGTCTACGA+AGG	0.649710	2.2:+62356929	None:intergenic
AGGTTTGGAGGAGACCAACA+TGG	0.658294	2.2:+62357443	None:intergenic
ACTGGTGATAGAGGTCTCTG+TGG	0.722605	2.2:-62357063	MS.gene02393:CDS
AGACCAACCCAAATTCAGTG+TGG	0.755194	2.2:-62357327	MS.gene02393:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTGAAAAAAGCTGAAAAT+AGG	-	chr2.2:62356799-62356818	MS.gene02393:CDS	20.0%
!	ATTTGACTGTTCAAATAAAG+TGG	+	chr2.2:62357281-62357300	None:intergenic	25.0%
!!!	ATTATCAGTGTTGGTAAAAA+GGG	-	chr2.2:62356858-62356877	MS.gene02393:CDS	25.0%
!!!	TATTATCAGTGTTGGTAAAA+AGG	-	chr2.2:62356857-62356876	MS.gene02393:CDS	25.0%
	AACCTATCAACAGGTATATA+AGG	+	chr2.2:62356906-62356925	None:intergenic	30.0%
	AACTTCTTCACTAACAAAGA+GGG	+	chr2.2:62356991-62357010	None:intergenic	30.0%
	AGTTGAAGAAGAATTTGTCA+AGG	-	chr2.2:62357384-62357403	MS.gene02393:CDS	30.0%
	ATTTGAACAGTCAAATCTTG+AGG	-	chr2.2:62357285-62357304	MS.gene02393:CDS	30.0%
	GATTACACTATTATCAGTGT+TGG	-	chr2.2:62356849-62356868	MS.gene02393:CDS	30.0%
	GGTGAAATCTGTGATATTAA+TGG	-	chr2.2:62357098-62357117	MS.gene02393:CDS	30.0%
	GTTGAAGAAGAATTTGTCAA+GGG	-	chr2.2:62357385-62357404	MS.gene02393:CDS	30.0%
	TTTGAACAGTCAAATCTTGA+GGG	-	chr2.2:62357286-62357305	MS.gene02393:CDS	30.0%
!	ATATCACAGATTTCACCTTT+TGG	+	chr2.2:62357095-62357114	None:intergenic	30.0%
!!	TGTTTTTGACAGAGTCAATA+CGG	+	chr2.2:62356539-62356558	None:intergenic	30.0%
	CAACTTCTTCACTAACAAAG+AGG	+	chr2.2:62356992-62357011	None:intergenic	35.0%
	GACCTTATATACCTGTTGAT+AGG	-	chr2.2:62356901-62356920	MS.gene02393:CDS	35.0%
	TCTTAGACCACACTGAATTT+GGG	+	chr2.2:62356505-62356524	None:intergenic	35.0%
	TTAGATGCATCAGGTAATGA+AGG	+	chr2.2:62356402-62356421	None:intergenic	35.0%
	TTGCTCAAATATCACCATGT+TGG	-	chr2.2:62356365-62356384	MS.gene02393:CDS	35.0%
	TTTGTGAAGGAAGCTGAAAA+GGG	+	chr2.2:62356449-62356468	None:intergenic	35.0%
!	ATTGCTGAGTTGAAAGTTCT+TGG	-	chr2.2:62356822-62356841	MS.gene02393:CDS	35.0%
!	GGAAGCAAAGTATGGATTAT+AGG	+	chr2.2:62357065-62357084	None:intergenic	35.0%
!	TTCAGGTTAACAACGAAAGA+AGG	-	chr2.2:62357152-62357171	MS.gene02393:CDS	35.0%
!	TTTTGTGAAGGAAGCTGAAA+AGG	+	chr2.2:62356450-62356469	None:intergenic	35.0%
!!	GAAGATGATGAGTTTTGTGA+AGG	+	chr2.2:62356462-62356481	None:intergenic	35.0%
!!!	ACAAGAGCAACTTTTTTCAC+TGG	+	chr2.2:62356732-62356751	None:intergenic	35.0%
	AGTGCATCAAGAATCTGAAC+AGG	+	chr2.2:62357254-62357273	None:intergenic	40.0%
	ATCAGGTAATGAAGGTTTGG+AGG	+	chr2.2:62356394-62356413	None:intergenic	40.0%
	CAGGTATATAAGGTCTACGA+AGG	+	chr2.2:62356896-62356915	None:intergenic	40.0%
	CCTTCAAGAAACCTATCAAC+AGG	+	chr2.2:62356915-62356934	None:intergenic	40.0%
	CTCTTAGACCACACTGAATT+TGG	+	chr2.2:62356506-62356525	None:intergenic	40.0%
	CTGTTGAAAGAGATGCTGTT+AGG	-	chr2.2:62357183-62357202	MS.gene02393:CDS	40.0%
	GAAATCCTGGAAATTGTTGC+TGG	-	chr2.2:62357443-62357462	MS.gene02393:CDS	40.0%
	GCTCAAGAAGCTGTTGTTAA+TGG	-	chr2.2:62356612-62356631	MS.gene02393:CDS	40.0%
	GTTTGTGCTTCTTTAGCAGT+AGG	+	chr2.2:62356948-62356967	None:intergenic	40.0%
	TAAGATCACTGGTGAAATCC+TGG	-	chr2.2:62357430-62357449	MS.gene02393:CDS	40.0%
	TCAACTAGTGTCTGTGAGAA+AGG	+	chr2.2:62356642-62356661	None:intergenic	40.0%
	TCCTGCTCAAACTGCAAAAT+CGG	+	chr2.2:62357230-62357249	None:intergenic	40.0%
	TGCATCAGGTAATGAAGGTT+TGG	+	chr2.2:62356397-62356416	None:intergenic	40.0%
	TTTCACCAGTGATCTTAGCT+TGG	+	chr2.2:62357427-62357446	None:intergenic	40.0%
!	ACTGGTCTAAGCTTAGTTAG+AGG	+	chr2.2:62356714-62356733	None:intergenic	40.0%
!	CCTGTTGATAGGTTTCTTGA+AGG	-	chr2.2:62356912-62356931	MS.gene02393:CDS	40.0%
!	GTTGATAGGTTTCTTGAAGG+TGG	-	chr2.2:62356915-62356934	MS.gene02393:CDS	40.0%
!!	ATTTCACCTTTTGGTGACAG+TGG	+	chr2.2:62357086-62357105	None:intergenic	40.0%
!!	TGAATTTGGGTTGGTCTTGA+AGG	+	chr2.2:62356492-62356511	None:intergenic	40.0%
	AAGGAGAGGTTAGATGCATC+AGG	+	chr2.2:62356411-62356430	None:intergenic	45.0%
	AGACCAACCCAAATTCAGTG+TGG	-	chr2.2:62356495-62356514	MS.gene02393:CDS	45.0%
	AGACCACACTGAATTTGGGT+TGG	+	chr2.2:62356501-62356520	None:intergenic	45.0%
	CACTGAAGCAATGAAGCTTG+TGG	-	chr2.2:62356569-62356588	MS.gene02393:CDS	45.0%
	GCTGTTCGTTGATGCTGTAA+AGG	+	chr2.2:62356668-62356687	None:intergenic	45.0%
	GTGACAGTGGAAGCAAAGTA+TGG	+	chr2.2:62357073-62357092	None:intergenic	45.0%
	GTGCTTCTTTAGCAGTAGGA+AGG	+	chr2.2:62356944-62356963	None:intergenic	45.0%
	TAGAGGAGCTTCGATATCGT+CGG	+	chr2.2:62356697-62356716	None:intergenic	45.0%
	TCAAATCTTGAGGGCTTTGC+AGG	-	chr2.2:62357295-62357314	MS.gene02393:CDS	45.0%
	TTGCTTCCACTGTCACCAAA+AGG	-	chr2.2:62357077-62357096	MS.gene02393:CDS	45.0%
!	AAACTGTGTTGATGCTGCTG+AGG	-	chr2.2:62357121-62357140	MS.gene02393:CDS	45.0%
!	GTTGTTTGCACTGGTGATAG+AGG	-	chr2.2:62356750-62356769	MS.gene02393:CDS	45.0%
!!	GTTGCTCTTGTTGTTTGCAC+TGG	-	chr2.2:62356741-62356760	MS.gene02393:CDS	45.0%
	ACTGGTGATAGAGGTCTCTG+TGG	-	chr2.2:62356759-62356778	MS.gene02393:CDS	50.0%
	AGGTTTGGAGGAGACCAACA+TGG	+	chr2.2:62356382-62356401	None:intergenic	50.0%
	CAACGCCAAGCTAAGATCAC+TGG	-	chr2.2:62357419-62357438	MS.gene02393:CDS	50.0%
	CAGCACCAGCAACAATTTCC+AGG	+	chr2.2:62357451-62357470	None:intergenic	50.0%
	GAAAAGGGCTGAGAGCTGAA+CGG	+	chr2.2:62356434-62356453	None:intergenic	50.0%
!	CTGCTGAGGATGAGCTTTTC+AGG	-	chr2.2:62357135-62357154	MS.gene02393:CDS	50.0%
!!	GCCGATTTTGCAGTTTGAGC+AGG	-	chr2.2:62357226-62357245	MS.gene02393:CDS	50.0%
	GGTGATAGAGGTCTCTGTGG+TGG	-	chr2.2:62356762-62356781	MS.gene02393:CDS	55.0%
	TGAGAGCTGAACGGAAGGAG+AGG	+	chr2.2:62356425-62356444	None:intergenic	55.0%
!!	TGTTGCTGGTGCTGATGCCT+TGG	-	chr2.2:62357457-62357476	MS.gene02393:CDS	55.0%
	AGGGCTGAGAGCTGAACGGA+AGG	+	chr2.2:62356430-62356449	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	62356360	62357484	62356360	ID=MS.gene02393
chr2.2	mRNA	62356360	62357484	62356360	ID=MS.gene02393.t1;Parent=MS.gene02393
chr2.2	exon	62356360	62357484	62356360	ID=MS.gene02393.t1.exon1;Parent=MS.gene02393.t1
chr2.2	CDS	62356360	62357484	62356360	ID=cds.MS.gene02393.t1;Parent=MS.gene02393.t1

Gene Sequence

>MS.gene02393

ATGTCTTGCTCAAATATCACCATGTTGGTCTCCTCCAAACCTTCATTACCTGATGCATCTAACCTCTCCTTCCGTTCAGCTCTCAGCCCTTTTCAGCTTCCTTCACAAAACTCATCATCTTCTTGCACTCCTTCAAGACCAACCCAAATTCAGTGTGGTCTAAGAGAACTTAGAAACCGTATTGACTCTGTCAAAAACACTCAGAAAATCACTGAAGCAATGAAGCTTGTGGCTGCTGCAAAAGTCAGAAGAGCTCAAGAAGCTGTTGTTAATGGCAGACCTTTCTCACAGACACTAGTTGAAGTCCTTTACAGCATCAACGAACAGCTTCAAACCGACGATATCGAAGCTCCTCTAACTAAGCTTAGACCAGTGAAAAAAGTTGCTCTTGTTGTTTGCACTGGTGATAGAGGTCTCTGTGGTGGATTCAACAATGCAATATTGAAAAAAGCTGAAAATAGGATTGCTGAGTTGAAAGTTCTTGGACTTGATTACACTATTATCAGTGTTGGTAAAAAGGGTAACTCTTATTTCCTTCGTAGACCTTATATACCTGTTGATAGGTTTCTTGAAGGTGGAAACCTTCCTACTGCTAAAGAAGCACAAACTATTGCTGATGATGTTTTCTCCCTCTTTGTTAGTGAAGAAGTTGATAAAGTTGAACTATTGTACACTAAATTTGTGTCATTAGTGAAATCAGAGCCTATAATCCATACTTTGCTTCCACTGTCACCAAAAGGTGAAATCTGTGATATTAATGGAAACTGTGTTGATGCTGCTGAGGATGAGCTTTTCAGGTTAACAACGAAAGAAGGAAAGTTAACTGTTGAAAGAGATGCTGTTAGGTCTAAGACGATTGATTTTTCGCCGATTTTGCAGTTTGAGCAGGATCCTGTTCAGATTCTTGATGCACTTTTGCCACTTTATTTGAACAGTCAAATCTTGAGGGCTTTGCAGGAATCACTTGCTAGTGAATTAGCTGCTAGAATGAGTGCTATGAGTAGTGCTTGTGATAATGCTATTGAGTTGAAGAAGAATTTGTCAAGGGTTTATAATAGACAACGCCAAGCTAAGATCACTGGTGAAATCCTGGAAATTGTTGCTGGTGCTGATGCCTTGGTATAG

Protein sequence

>MS.gene02393.t1

MSCSNITMLVSSKPSLPDASNLSFRSALSPFQLPSQNSSSSCTPSRPTQIQCGLRELRNRIDSVKNTQKITEAMKLVAAAKVRRAQEAVVNGRPFSQTLVEVLYSINEQLQTDDIEAPLTKLRPVKKVALVVCTGDRGLCGGFNNAILKKAENRIAELKVLGLDYTIISVGKKGNSYFLRRPYIPVDRFLEGGNLPTAKEAQTIADDVFSLFVSEEVDKVELLYTKFVSLVKSEPIIHTLLPLSPKGEICDINGNCVDAAEDELFRLTTKEGKLTVERDAVRSKTIDFSPILQFEQDPVQILDALLPLYLNSQILRALQESLASELAARMSAMSSACDNAIELKKNLSRVYNRQRQAKITGEILEIVAGADALV