AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024426

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024426.t1 MTR_7g117670 93.664 584 21 5 1 578 1 574 0.0 1031
MS.gene024426.t1 MTR_8g027495 57.686 631 181 17 8 571 12 623 0.0 619
MS.gene024426.t1 MTR_7g096350 36.522 575 266 21 10 566 9 502 1.13e-97 306
MS.gene024426.t1 MTR_5g030430 41.704 223 105 5 372 572 458 677 4.26e-42 161
MS.gene024426.t1 MTR_5g030430 27.119 236 154 3 8 239 3 224 6.34e-21 97.4
MS.gene024426.t1 MTR_8g067280 42.365 203 85 5 388 565 444 639 2.14e-40 156
MS.gene024426.t1 MTR_8g067280 31.944 216 115 9 48 252 52 246 3.80e-17 85.5
MS.gene024426.t1 MTR_8g024790 38.372 172 97 1 386 548 271 442 2.11e-33 133
MS.gene024426.t1 MTR_8g024770 37.566 189 104 4 386 561 259 446 6.88e-31 125
MS.gene024426.t1 MTR_5g030420 30.049 203 124 4 42 240 20 208 1.77e-18 85.5
MS.gene024426.t1 MTR_5g014600 30.058 173 98 2 382 532 125 296 1.97e-18 87.0
MS.gene024426.t1 MTR_5g030420 30.049 203 124 4 42 240 20 208 2.43e-18 85.5
MS.gene024426.t1 MTR_4g097950 37.097 124 57 2 406 511 70 190 1.62e-17 82.8
MS.gene024426.t1 MTR_5g014640 37.302 126 68 4 393 513 145 264 2.25e-16 80.9
MS.gene024426.t1 MTR_4g067010 31.515 165 103 3 393 551 137 297 4.74e-16 80.1
MS.gene024426.t1 MTR_5g014520 28.108 185 122 4 389 564 142 324 6.29e-16 79.7
MS.gene024426.t1 MTR_2g010450 29.412 136 87 1 406 532 177 312 7.02e-16 79.7
MS.gene024426.t1 MTR_4g092700 57.627 59 25 0 404 462 156 214 7.49e-16 79.3
MS.gene024426.t1 MTR_2g104550 33.333 153 74 4 384 508 97 249 7.50e-16 79.3
MS.gene024426.t1 MTR_5g014560 27.957 186 112 4 393 563 119 297 1.16e-15 78.6
MS.gene024426.t1 MTR_0011s0210 32.298 161 97 4 399 551 180 336 1.44e-14 75.9
MS.gene024426.t1 MTR_0250s0040 32.143 168 98 6 383 541 71 231 3.00e-14 73.6
MS.gene024426.t1 MTR_0246s0050 29.651 172 107 4 387 553 136 298 3.28e-14 74.3
MS.gene024426.t1 MTR_4g098035 29.651 172 107 4 387 553 157 319 3.52e-14 74.3
MS.gene024426.t1 MTR_1g072320 22.335 197 132 6 50 240 7 188 3.75e-14 75.9
MS.gene024426.t1 MTR_1g072320 46.512 86 44 2 390 473 453 538 8.01e-14 75.1
MS.gene024426.t1 MTR_4g066380 49.333 75 34 1 387 461 138 208 4.00e-14 74.3
MS.gene024426.t1 MTR_6g047550 34.532 139 76 4 328 463 104 230 4.52e-14 73.2
MS.gene024426.t1 MTR_0246s0020 51.429 70 28 2 392 461 190 253 6.21e-14 73.9
MS.gene024426.t1 MTR_0246s0020 51.429 70 28 2 392 461 190 253 7.35e-14 73.9
MS.gene024426.t1 MTR_2g084280 31.333 150 97 3 410 553 137 286 7.56e-14 73.2
MS.gene024426.t1 MTR_8g098275 52.308 65 31 0 402 466 240 304 7.57e-14 74.3
MS.gene024426.t1 MTR_0011s0260 31.677 161 98 3 399 551 180 336 8.36e-14 73.6
MS.gene024426.t1 MTR_3g498695 52.239 67 32 0 404 470 296 362 1.11e-13 73.9
MS.gene024426.t1 MTR_8g098275 52.308 65 31 0 402 466 304 368 1.13e-13 73.9
MS.gene024426.t1 MTR_3g498695 52.239 67 32 0 404 470 296 362 1.16e-13 73.9
MS.gene024426.t1 MTR_4g097940 35.897 117 66 3 395 506 148 260 1.18e-13 72.8
MS.gene024426.t1 MTR_8g098275 52.308 65 31 0 402 466 397 461 1.50e-13 73.9
MS.gene024426.t1 MTR_8g098275 28.889 180 108 7 65 238 17 182 6.43e-12 68.9
MS.gene024426.t1 MTR_2g038040 58.182 55 23 0 407 461 53 107 1.66e-13 70.9
MS.gene024426.t1 MTR_4g057270 30.588 170 105 5 396 553 140 308 1.77e-13 72.4
MS.gene024426.t1 MTR_1g112680 41.053 95 52 2 369 463 284 374 2.13e-13 73.2
MS.gene024426.t1 MTR_6g047570 26.423 246 158 7 313 547 75 308 2.59e-13 71.6
MS.gene024426.t1 MTR_4g066460 49.333 75 34 1 387 461 140 210 3.07e-13 71.6
MS.gene024426.t1 MTR_4g009110 26.702 191 106 5 333 508 116 287 4.61e-13 71.2
MS.gene024426.t1 MTR_7g083900 54.098 61 28 0 404 464 284 344 5.60e-13 71.6
MS.gene024426.t1 MTR_7g083900 54.098 61 28 0 404 464 284 344 5.67e-13 71.6
MS.gene024426.t1 MTR_2g104490 33.600 125 68 3 394 508 126 245 6.02e-13 70.1
MS.gene024426.t1 MTR_2g104490 33.600 125 68 3 394 508 126 245 6.77e-13 70.1
MS.gene024426.t1 MTR_4g009540 23.762 202 139 3 380 566 126 327 1.93e-12 68.9
MS.gene024426.t1 MTR_2g022280 26.404 178 108 3 405 560 1 177 2.43e-12 66.6
MS.gene024426.t1 MTR_4g009540 26.573 143 91 2 380 508 126 268 5.45e-12 67.4
MS.gene024426.t1 MTR_2g104560 25.000 168 119 3 407 567 55 222 5.82e-12 66.2
MS.gene024426.t1 MTR_4g097920 46.154 65 31 1 395 459 133 193 1.92e-11 65.9
MS.gene024426.t1 MTR_3g030700 43.662 71 40 0 394 464 184 254 2.13e-11 66.2
MS.gene024426.t1 MTR_4g097920 46.154 65 31 1 395 459 148 208 2.14e-11 65.9
MS.gene024426.t1 MTR_3g104100 48.387 62 29 1 410 471 199 257 5.44e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024426.t1 AT1G01260 48.214 616 247 15 4 577 3 588 1.43e-176 512
MS.gene024426.t1 AT1G01260 48.214 616 247 15 4 577 3 588 1.43e-176 512
MS.gene024426.t1 AT1G01260 48.214 616 247 15 4 577 3 588 1.43e-176 512
MS.gene024426.t1 AT2G46510 47.555 593 234 18 9 572 8 552 5.46e-163 477
MS.gene024426.t1 AT4G17880 32.914 556 301 17 48 565 61 582 1.90e-72 243
MS.gene024426.t1 AT4G16430 49.730 185 74 3 385 566 299 467 1.66e-53 189
MS.gene024426.t1 AT4G16430 37.500 240 120 6 10 240 8 226 9.27e-37 143
MS.gene024426.t1 AT5G46760 44.444 216 106 5 361 564 371 584 2.11e-45 169
MS.gene024426.t1 AT1G32640 44.211 190 90 3 387 564 433 618 1.02e-38 150
MS.gene024426.t1 AT1G32640 32.828 198 117 4 48 240 66 252 8.49e-20 94.0
MS.gene024426.t1 AT5G46830 30.080 502 272 18 67 548 49 491 1.14e-36 143
MS.gene024426.t1 AT5G57150 33.645 214 111 8 386 578 40 243 3.03e-20 90.9
MS.gene024426.t1 AT5G57150 33.889 180 96 6 386 548 40 213 8.97e-19 85.9
MS.gene024426.t1 AT5G57150 33.889 180 96 6 386 548 41 214 1.44e-18 85.1
MS.gene024426.t1 AT5G57150 33.889 180 96 6 386 548 41 214 1.54e-18 85.5
MS.gene024426.t1 AT5G57150 33.889 180 96 6 386 548 41 214 1.62e-18 86.3
MS.gene024426.t1 AT4G00870 37.500 136 74 1 405 529 248 383 2.54e-18 88.2
MS.gene024426.t1 AT4G37850 28.194 227 123 5 358 556 98 312 3.43e-18 86.7
MS.gene024426.t1 AT4G37850 28.194 227 123 5 358 556 98 312 3.43e-18 86.7
MS.gene024426.t1 AT4G09820 28.194 227 145 5 36 253 2 219 5.28e-18 87.8
MS.gene024426.t1 AT4G09820 34.351 131 55 3 382 508 350 453 5.42e-16 81.6
MS.gene024426.t1 AT5G41315 29.167 216 127 4 41 240 1 206 1.47e-16 83.6
MS.gene024426.t1 AT5G41315 48.571 70 36 0 400 469 435 504 8.48e-14 74.7
MS.gene024426.t1 AT5G41315 29.167 216 127 4 41 240 6 211 1.59e-16 83.6
MS.gene024426.t1 AT5G41315 48.571 70 36 0 400 469 440 509 9.31e-14 74.7
MS.gene024426.t1 AT5G41315 29.167 216 127 4 41 240 9 214 1.61e-16 83.6
MS.gene024426.t1 AT5G41315 48.571 70 36 0 400 469 443 512 9.18e-14 74.7
MS.gene024426.t1 AT1G63650 29.148 223 128 8 41 247 1 209 1.75e-15 80.1
MS.gene024426.t1 AT1G63650 48.529 68 35 0 402 469 401 468 1.54e-13 73.9
MS.gene024426.t1 AT1G63650 29.148 223 128 8 41 247 1 209 1.75e-15 80.1
MS.gene024426.t1 AT1G63650 48.529 68 35 0 402 469 401 468 1.54e-13 73.9
MS.gene024426.t1 AT1G63650 29.148 223 128 8 41 247 1 209 1.75e-15 80.1
MS.gene024426.t1 AT1G63650 48.529 68 35 0 402 469 401 468 1.54e-13 73.9
MS.gene024426.t1 AT4G29930 31.757 148 94 3 390 536 43 184 1.50e-14 73.9
MS.gene024426.t1 AT4G29930 29.091 165 110 3 390 553 43 201 1.72e-14 73.6
MS.gene024426.t1 AT4G00480 29.798 198 106 7 67 243 36 221 2.68e-14 76.3
MS.gene024426.t1 AT2G22750 32.911 158 89 3 388 532 136 289 3.39e-14 74.3
MS.gene024426.t1 AT2G22750 32.911 158 89 3 388 532 140 293 3.40e-14 74.3
MS.gene024426.t1 AT2G22750 32.911 158 89 3 388 532 140 293 3.40e-14 74.3
MS.gene024426.t1 AT1G12860 53.731 67 31 0 404 470 265 331 3.51e-14 75.5
MS.gene024426.t1 AT2G22750 32.911 158 89 3 388 532 112 265 3.57e-14 73.9
MS.gene024426.t1 AT2G22750 32.911 158 89 3 388 532 112 265 3.60e-14 73.9
MS.gene024426.t1 AT4G00480 29.798 198 106 7 67 243 36 221 3.70e-14 75.9
MS.gene024426.t1 AT1G12860 53.731 67 31 0 404 470 265 331 3.72e-14 75.5
MS.gene024426.t1 AT4G00480 29.798 198 106 7 67 243 36 221 6.75e-14 75.1
MS.gene024426.t1 AT4G29930 28.144 167 112 4 390 553 43 204 7.63e-14 71.6
MS.gene024426.t1 AT2G16910 33.728 169 73 5 410 546 318 479 9.94e-14 74.3
MS.gene024426.t1 AT3G26744 55.738 61 27 0 404 464 305 365 2.19e-13 73.2
MS.gene024426.t1 AT3G26744 55.738 61 27 0 404 464 305 365 2.39e-13 73.2
MS.gene024426.t1 AT3G26744 55.738 61 27 0 404 464 305 365 2.39e-13 73.2
MS.gene024426.t1 AT3G26744 55.738 61 27 0 404 464 305 365 2.39e-13 73.2
MS.gene024426.t1 AT4G29930 48.611 72 32 1 390 461 43 109 2.51e-13 69.3
MS.gene024426.t1 AT4G29930 48.611 72 32 1 390 461 43 109 3.06e-13 70.5
MS.gene024426.t1 AT4G29930 48.611 72 32 1 390 461 43 109 3.06e-13 70.5
MS.gene024426.t1 AT4G29930 48.611 72 32 1 390 461 43 109 3.82e-13 70.1
MS.gene024426.t1 AT2G22760 33.628 113 64 2 407 508 120 232 4.48e-13 70.5
MS.gene024426.t1 AT2G22760 33.628 113 64 2 407 508 120 232 5.22e-13 70.5
MS.gene024426.t1 AT2G22770 50.000 66 30 1 400 462 123 188 5.05e-12 67.8
MS.gene024426.t1 AT2G28160 45.312 64 35 0 410 473 135 198 5.60e-12 67.4
MS.gene024426.t1 AT2G28160 45.312 64 35 0 410 473 135 198 6.47e-12 67.4
MS.gene024426.t1 AT5G65640 28.902 173 109 4 403 566 175 342 5.35e-11 65.1

Find 110 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATTCAACACAACTTT+TGG 0.177209 7.2:-781026 None:intergenic
ATTGGAATTACGCAATTTAT+TGG 0.206579 7.2:+780415 MS.gene024426:CDS
CTGACTTAATTACAAAATTA+TGG 0.220746 7.2:-781844 None:intergenic
TTACTGCTTTCGTTCTTTCT+TGG 0.235602 7.2:+780794 MS.gene024426:CDS
TGCGTAATTCCAATTGAAAT+TGG 0.241887 7.2:-780406 None:intergenic
ATGCCTATATGGACAAAGTT+TGG 0.244044 7.2:-781937 None:intergenic
TTCAACAAATCAAAGATTTC+AGG 0.251699 7.2:-780897 None:intergenic
CCGGGTCCACCATACCCTTT+TGG 0.264704 7.2:-780723 None:intergenic
CTCTATTTATCATGCCTATA+TGG 0.268867 7.2:-781948 None:intergenic
CAGAAGTTGCATAAAGCTTT+TGG 0.288502 7.2:+780612 MS.gene024426:CDS
GAAACTTGCCATTAGGAAAA+TGG 0.290252 7.2:+781073 MS.gene024426:CDS
GATGAAGACAATTACGCTTT+TGG 0.306225 7.2:+780642 MS.gene024426:CDS
TCTGCCTCCACATGATTTAG+AGG 0.320999 7.2:-781428 None:intergenic
GATCAATAAAGCGCATGATA+TGG 0.340119 7.2:+780223 MS.gene024426:CDS
TGCCTATATGGACAAAGTTT+GGG 0.350641 7.2:-781936 None:intergenic
ATTCTTCATCCAATTTCAAT+TGG 0.357065 7.2:+780397 MS.gene024426:CDS
ACCAGAAGCAAAACACTTAC+CGG 0.359802 7.2:-780742 None:intergenic
TTGTCCATCTTGGAAATATT+CGG 0.360963 7.2:-781506 None:intergenic
TTAGGGAGAAACTTGCCATT+AGG 0.364646 7.2:+781066 MS.gene024426:CDS
GGACAAAGTTTGGGTTTGTA+AGG 0.367197 7.2:-781927 None:intergenic
AATAACCGGTCTCGCAGAAT+TGG 0.375253 7.2:-781306 None:intergenic
CGACTTTCTAAGTCAAAATC+TGG 0.375807 7.2:+780438 MS.gene024426:CDS
TGTTCTTGGTGCTCGAGCTT+TGG 0.376498 7.2:+780275 MS.gene024426:CDS
ACCATGGTTGGAGCTGTTCT+TGG 0.376666 7.2:+780261 MS.gene024426:CDS
ACTCCACCTTCTTCTTCGTT+TGG 0.377124 7.2:-780501 None:intergenic
CTGGTGATTACGTTCTTTGT+TGG 0.387307 7.2:+780457 MS.gene024426:CDS
TGCATTGGCATCTGCCTTTG+TGG 0.387976 7.2:-781254 None:intergenic
CTAGGTCTCCTTTCATCGCT+AGG 0.390719 7.2:-781368 None:intergenic
CATTTGGAAGCAGTACTTCC+AGG 0.403231 7.2:+781609 MS.gene024426:CDS
GTTTCGATTAGTTTCGAAAC+AGG 0.406082 7.2:-781758 None:intergenic
ATTCGGTACAACAGCTCTTA+AGG 0.408610 7.2:-781489 None:intergenic
ATGGTATTTGTGGTTCTACT+TGG 0.409907 7.2:+781144 MS.gene024426:CDS
AACTTTCCTAGTGCTAGAAA+TGG 0.411288 7.2:+781125 MS.gene024426:CDS
TCTTTCTTGGCTAAATCTGC+TGG 0.412934 7.2:+780807 MS.gene024426:CDS
CGCGGAAGGAAGCCTGCTAA+TGG 0.414504 7.2:+781395 MS.gene024426:CDS
TGGTGATTACGTTCTTTGTT+GGG 0.415145 7.2:+780458 MS.gene024426:CDS
TAAAGATGCCTTGTCCATCT+TGG 0.422075 7.2:-781516 None:intergenic
TGAAACAAAGTATGAAGAAG+TGG 0.425466 7.2:+780583 MS.gene024426:CDS
TGCTTCTAATGGTGTGAGAA+AGG 0.436502 7.2:+781196 MS.gene024426:CDS
AGTTTGGGTTTGTAAGGAGT+TGG 0.441750 7.2:-781921 None:intergenic
TTGCTTCTGGTAAACATTTG+TGG 0.442625 7.2:+780754 MS.gene024426:CDS
GAATACTAGAGTTTCTCTTC+CGG 0.446637 7.2:+781670 MS.gene024426:CDS
TTGTAAGGAGTTGGATTCTC+GGG 0.450320 7.2:-781912 None:intergenic
GAGTCAGAGAGAGAATCATT+TGG 0.451334 7.2:+781593 MS.gene024426:CDS
ATGGACAAGGCATCTTTACT+TGG 0.451417 7.2:+781521 MS.gene024426:CDS
TAGAGTTTCTCTTCCGGATG+TGG 0.452720 7.2:+781676 MS.gene024426:CDS
GGTTCGTCCCTTCCATTAGC+AGG 0.455729 7.2:-781407 None:intergenic
GGCTTCCTTCCGCGCTTCCT+AGG 0.458293 7.2:-781386 None:intergenic
TGGTATTTGTGGTTCTACTT+GGG 0.461524 7.2:+781145 MS.gene024426:CDS
TACGTTCTTTGTTGGGCAGA+TGG 0.462040 7.2:+780465 MS.gene024426:CDS
AGTGCTAGAAATGGTATTTG+TGG 0.472584 7.2:+781134 MS.gene024426:CDS
TTTGTAAGGAGTTGGATTCT+CGG 0.473242 7.2:-781913 None:intergenic
CTTTCTTCATTGACCACACT+AGG 0.491428 7.2:-780978 None:intergenic
GAAGAAAGTACAGTTTCAAA+TGG 0.494078 7.2:+780993 MS.gene024426:CDS
TGCTGCAATCAACTTGTCCT+TGG 0.495738 7.2:-781885 None:intergenic
AATGATAATGATTGTGTGGC+AGG 0.499914 7.2:-780951 None:intergenic
TCAAGCGAAACTCAAGATCA+TGG 0.503060 7.2:+781571 MS.gene024426:CDS
ACGTTCTTTGTTGGGCAGAT+GGG 0.511903 7.2:+780466 MS.gene024426:CDS
AGTACTTCCAGGGATGGATC+AGG 0.512089 7.2:+781620 MS.gene024426:CDS
GAGGGCCAATTCTGCGAGAC+CGG 0.516559 7.2:+781301 MS.gene024426:CDS
TCAAGATGAGGTAATAGTAA+AGG 0.517416 7.2:+781712 MS.gene024426:CDS
AACCCAAACTTTGTCCATAT+AGG 0.517452 7.2:+781934 MS.gene024426:CDS
GGTATTTGTGGTTCTACTTG+GGG 0.519442 7.2:+781146 MS.gene024426:CDS
CGAGTTGCCTGATCCATCCC+TGG 0.522358 7.2:-781627 None:intergenic
CCGGAAAAGTCAATTTGCAT+TGG 0.525652 7.2:-781269 None:intergenic
GAGACCGGTTATTGCTGAAT+CGG 0.527481 7.2:+781316 MS.gene024426:CDS
TTGGCATCTGCCTTTGTGGT+TGG 0.531676 7.2:-781250 None:intergenic
ATTGGCCCTCGAATTTGCTC+CGG 0.532064 7.2:-781288 None:intergenic
GTTAGAGAAACTCTGAGTCT+TGG 0.536388 7.2:+780522 MS.gene024426:CDS
AGTTCCGATTCAGCAATAAC+CGG 0.536707 7.2:-781320 None:intergenic
CCAAAAGGGTATGGTGGACC+CGG 0.545307 7.2:+780723 MS.gene024426:CDS
GGAAGCAGTACTTCCAGGGA+TGG 0.552030 7.2:+781614 MS.gene024426:CDS
TGTACCGAATATTTCCAAGA+TGG 0.552909 7.2:+781502 MS.gene024426:CDS
GAAGCTTGAATATCCACATC+CGG 0.553300 7.2:-781689 None:intergenic
ATGTGGAGGCAGAGAGGCAA+AGG 0.553319 7.2:+781438 MS.gene024426:CDS
CCAGAAGCAAAACACTTACC+GGG 0.554206 7.2:-780741 None:intergenic
GAAGAAGAGAAAACCATGGT+TGG 0.555055 7.2:+780249 MS.gene024426:CDS
GAAACAAAGTATGAAGAAGT+GGG 0.558139 7.2:+780584 MS.gene024426:CDS
TCTACTTGGGGAGTGAATCA+AGG 0.559151 7.2:+781158 MS.gene024426:CDS
GAAGGAAGCCTGCTAATGGA+AGG 0.564326 7.2:+781399 MS.gene024426:CDS
ATTTGGAAGCAGTACTTCCA+GGG 0.565753 7.2:+781610 MS.gene024426:CDS
CTGATCAACCTAGCGATGAA+AGG 0.566880 7.2:+781360 MS.gene024426:CDS
AACTGTTTATCAAATGAGAG+TGG 0.571748 7.2:+780312 MS.gene024426:CDS
CAAATACCATTTCTAGCACT+AGG 0.582823 7.2:-781131 None:intergenic
GGAGACCTAGGAAGCGCGGA+AGG 0.585153 7.2:+781381 MS.gene024426:CDS
GAGAGTGGATTGATTGCTGT+TGG 0.590794 7.2:+780327 MS.gene024426:CDS
CTGTGTCGGTTACACGGTCA+AGG 0.590950 7.2:-780665 None:intergenic
ACTAATCGAAACATTCAAAG+AGG 0.592504 7.2:+781769 MS.gene024426:CDS
ACATCTCTGTGTCGGTTACA+CGG 0.595335 7.2:-780671 None:intergenic
TCTGAGTCTTGGCATAGAAG+AGG 0.598956 7.2:+780533 MS.gene024426:CDS
ACCAAGAACAGCTCCAACCA+TGG 0.601753 7.2:-780262 None:intergenic
AGAGAACCAAACGAAGAAGA+AGG 0.608578 7.2:+780495 MS.gene024426:CDS
ATTATCATTGTATCCTAGTG+TGG 0.610408 7.2:+780965 MS.gene024426:CDS
GGAACTTGTTGCTGATGTTG+AGG 0.613094 7.2:+781337 MS.gene024426:CDS
GAACCAAACGAAGAAGAAGG+TGG 0.613103 7.2:+780498 MS.gene024426:CDS
CTAGCGATGAAAGGAGACCT+AGG 0.617300 7.2:+781369 MS.gene024426:CDS
TATTCAAGCTTCTCAAGATG+AGG 0.619645 7.2:+781700 MS.gene024426:CDS
TCAACAACACCAAAATCAGT+AGG 0.632462 7.2:-780852 None:intergenic
TGGGGAGTGAATCAAGGTCA+AGG 0.636279 7.2:+781164 MS.gene024426:CDS
GTTGTGTTGAATTTGAACAA+TGG 0.637607 7.2:+781032 MS.gene024426:CDS
GGACGAACCTCTAAATCATG+TGG 0.640327 7.2:+781421 MS.gene024426:CDS
AAGGAAGCCTGCTAATGGAA+GGG 0.640978 7.2:+781400 MS.gene024426:CDS
GAAAGGAGACCTAGGAAGCG+CGG 0.657593 7.2:+781377 MS.gene024426:CDS
GAATATTTCCAAGATGGACA+AGG 0.676800 7.2:+781508 MS.gene024426:CDS
ATACAATGATAATGATTGTG+TGG 0.679020 7.2:-780955 None:intergenic
TAAATCATGTGGAGGCAGAG+AGG 0.679660 7.2:+781432 MS.gene024426:CDS
CGAACCTCTAAATCATGTGG+AGG 0.682050 7.2:+781424 MS.gene024426:CDS
AGGATCTGAACAGCTTACCA+AGG 0.700634 7.2:+781868 MS.gene024426:CDS
ATAGCAACTACCAACCACAA+AGG 0.712491 7.2:+781240 MS.gene024426:CDS
GAATGAAGAAGAGAAAACCA+TGG 0.720626 7.2:+780245 MS.gene024426:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTGACTTAATTACAAAATTA+TGG - chr7.2:781847-781866 None:intergenic 20.0%
!!! AATTTTGTAATTAAGTCAGA+AGG + chr7.2:781848-781867 MS.gene024426:CDS 20.0%
! ATTCTTCATCCAATTTCAAT+TGG + chr7.2:780397-780416 MS.gene024426:CDS 25.0%
! ATTGGAATTACGCAATTTAT+TGG + chr7.2:780415-780434 MS.gene024426:CDS 25.0%
! TTCAACAAATCAAAGATTTC+AGG - chr7.2:780900-780919 None:intergenic 25.0%
!! ATACAATGATAATGATTGTG+TGG - chr7.2:780958-780977 None:intergenic 25.0%
!! ATTACTATCCATTTTCCTAA+TGG - chr7.2:781084-781103 None:intergenic 25.0%
!! TTCAAATTCAACACAACTTT+TGG - chr7.2:781029-781048 None:intergenic 25.0%
AAAGAAGAAGAAGAAGAAGA+AGG + chr7.2:780558-780577 MS.gene024426:CDS 30.0%
AACTGTTTATCAAATGAGAG+TGG + chr7.2:780312-780331 MS.gene024426:CDS 30.0%
ACTAATCGAAACATTCAAAG+AGG + chr7.2:781769-781788 MS.gene024426:CDS 30.0%
ATTATCATTGTATCCTAGTG+TGG + chr7.2:780965-780984 MS.gene024426:CDS 30.0%
GAAACAAAGTATGAAGAAGT+GGG + chr7.2:780584-780603 MS.gene024426:CDS 30.0%
GTTGTGTTGAATTTGAACAA+TGG + chr7.2:781032-781051 MS.gene024426:CDS 30.0%
TAAAAAGAACATCTCTGTGT+CGG - chr7.2:780682-780701 None:intergenic 30.0%
TCAAGATGAGGTAATAGTAA+AGG + chr7.2:781712-781731 MS.gene024426:CDS 30.0%
TGAAACAAAGTATGAAGAAG+TGG + chr7.2:780583-780602 MS.gene024426:CDS 30.0%
TGCGTAATTCCAATTGAAAT+TGG - chr7.2:780409-780428 None:intergenic 30.0%
TTGTCCATCTTGGAAATATT+CGG - chr7.2:781509-781528 None:intergenic 30.0%
! ATGTACTTCTTTTTCCCAAA+AGG + chr7.2:780708-780727 MS.gene024426:CDS 30.0%
! GAAGAAAGTACAGTTTCAAA+TGG + chr7.2:780993-781012 MS.gene024426:CDS 30.0%
! TGTACTTCTTTTTCCCAAAA+GGG + chr7.2:780709-780728 MS.gene024426:CDS 30.0%
!!! GGTGAATTTTTTGCTTCTAA+TGG + chr7.2:781185-781204 MS.gene024426:CDS 30.0%
!!! GTTTTAGTACCTACTGATTT+TGG + chr7.2:780843-780862 MS.gene024426:CDS 30.0%
AACTTTCCTAGTGCTAGAAA+TGG + chr7.2:781125-781144 MS.gene024426:CDS 35.0%
AGTGCTAGAAATGGTATTTG+TGG + chr7.2:781134-781153 MS.gene024426:CDS 35.0%
CGACTTTCTAAGTCAAAATC+TGG + chr7.2:780438-780457 MS.gene024426:CDS 35.0%
GAAACTTGCCATTAGGAAAA+TGG + chr7.2:781073-781092 MS.gene024426:CDS 35.0%
GAATACTAGAGTTTCTCTTC+CGG + chr7.2:781670-781689 MS.gene024426:CDS 35.0%
GAATATTTCCAAGATGGACA+AGG + chr7.2:781508-781527 MS.gene024426:CDS 35.0%
GAATGAAGAAGAGAAAACCA+TGG + chr7.2:780245-780264 MS.gene024426:CDS 35.0%
GATCAATAAAGCGCATGATA+TGG + chr7.2:780223-780242 MS.gene024426:CDS 35.0%
TATTCAAGCTTCTCAAGATG+AGG + chr7.2:781700-781719 MS.gene024426:CDS 35.0%
TCAACAACACCAAAATCAGT+AGG - chr7.2:780855-780874 None:intergenic 35.0%
TGGTGATTACGTTCTTTGTT+GGG + chr7.2:780458-780477 MS.gene024426:CDS 35.0%
TGTACCGAATATTTCCAAGA+TGG + chr7.2:781502-781521 MS.gene024426:CDS 35.0%
TTACTGCTTTCGTTCTTTCT+TGG + chr7.2:780794-780813 MS.gene024426:CDS 35.0%
TTGCTTCTGGTAAACATTTG+TGG + chr7.2:780754-780773 MS.gene024426:CDS 35.0%
TTTGTAAGGAGTTGGATTCT+CGG - chr7.2:781916-781935 None:intergenic 35.0%
! AAGTTGCATAAAGCTTTTGG+TGG + chr7.2:780615-780634 MS.gene024426:CDS 35.0%
! AATGATAATGATTGTGTGGC+AGG - chr7.2:780954-780973 None:intergenic 35.0%
! ACAATGGTCGTACACATTTT+AGG + chr7.2:781048-781067 MS.gene024426:CDS 35.0%
! ATGGTATTTGTGGTTCTACT+TGG + chr7.2:781144-781163 MS.gene024426:CDS 35.0%
! ATGGTGTGAGAAAGGATTTT+AGG + chr7.2:781204-781223 MS.gene024426:CDS 35.0%
! CAATGGTCGTACACATTTTA+GGG + chr7.2:781049-781068 MS.gene024426:CDS 35.0%
! CAGAAGTTGCATAAAGCTTT+TGG + chr7.2:780612-780631 MS.gene024426:CDS 35.0%
! GATGAAGACAATTACGCTTT+TGG + chr7.2:780642-780661 MS.gene024426:CDS 35.0%
! TGGTATTTGTGGTTCTACTT+GGG + chr7.2:781145-781164 MS.gene024426:CDS 35.0%
! TTCTTTTTCCCAAAAGGGTA+TGG + chr7.2:780714-780733 MS.gene024426:CDS 35.0%
!! CAAATACCATTTCTAGCACT+AGG - chr7.2:781134-781153 None:intergenic 35.0%
!! CCAATGCAAATTGACTTTTC+CGG + chr7.2:781269-781288 MS.gene024426:CDS 35.0%
!! GTTTCGATTAGTTTCGAAAC+AGG - chr7.2:781761-781780 None:intergenic 35.0%
!!! GATTTTGGTGTTGTTGAGTT+GGG + chr7.2:780858-780877 MS.gene024426:CDS 35.0%
!!! TGATTTTGGTGTTGTTGAGT+TGG + chr7.2:780857-780876 MS.gene024426:CDS 35.0%
ACCAGAAGCAAAACACTTAC+CGG - chr7.2:780745-780764 None:intergenic 40.0%
AGAGAACCAAACGAAGAAGA+AGG + chr7.2:780495-780514 MS.gene024426:CDS 40.0%
AGTTCCGATTCAGCAATAAC+CGG - chr7.2:781323-781342 None:intergenic 40.0%
ATAGCAACTACCAACCACAA+AGG + chr7.2:781240-781259 MS.gene024426:CDS 40.0%
ATTCGGTACAACAGCTCTTA+AGG - chr7.2:781492-781511 None:intergenic 40.0%
ATTTGGAAGCAGTACTTCCA+GGG + chr7.2:781610-781629 MS.gene024426:CDS 40.0%
CCGGAAAAGTCAATTTGCAT+TGG - chr7.2:781272-781291 None:intergenic 40.0%
CTGGTGATTACGTTCTTTGT+TGG + chr7.2:780457-780476 MS.gene024426:CDS 40.0%
CTTTCTTCATTGACCACACT+AGG - chr7.2:780981-781000 None:intergenic 40.0%
GAAGAAGAGAAAACCATGGT+TGG + chr7.2:780249-780268 MS.gene024426:CDS 40.0%
GAAGCTTGAATATCCACATC+CGG - chr7.2:781692-781711 None:intergenic 40.0%
GAGTCAGAGAGAGAATCATT+TGG + chr7.2:781593-781612 MS.gene024426:CDS 40.0%
GTTAGAGAAACTCTGAGTCT+TGG + chr7.2:780522-780541 MS.gene024426:CDS 40.0%
TAAAGATGCCTTGTCCATCT+TGG - chr7.2:781519-781538 None:intergenic 40.0%
TCAAGCGAAACTCAAGATCA+TGG + chr7.2:781571-781590 MS.gene024426:CDS 40.0%
TCTTTCTTGGCTAAATCTGC+TGG + chr7.2:780807-780826 MS.gene024426:CDS 40.0%
TGCTTCTAATGGTGTGAGAA+AGG + chr7.2:781196-781215 MS.gene024426:CDS 40.0%
TTAGGGAGAAACTTGCCATT+AGG + chr7.2:781066-781085 MS.gene024426:CDS 40.0%
TTGTAAGGAGTTGGATTCTC+GGG - chr7.2:781915-781934 None:intergenic 40.0%
! AGTTTGGGTTTGTAAGGAGT+TGG - chr7.2:781924-781943 None:intergenic 40.0%
! ATGGACAAGGCATCTTTACT+TGG + chr7.2:781521-781540 MS.gene024426:CDS 40.0%
! GGACAAAGTTTGGGTTTGTA+AGG - chr7.2:781930-781949 None:intergenic 40.0%
! GGTATTTGTGGTTCTACTTG+GGG + chr7.2:781146-781165 MS.gene024426:CDS 40.0%
!! TTTTTCCCAAAAGGGTATGG+TGG + chr7.2:780717-780736 MS.gene024426:CDS 40.0%
AAGGAAGCCTGCTAATGGAA+GGG + chr7.2:781400-781419 MS.gene024426:CDS 45.0%
AATAACCGGTCTCGCAGAAT+TGG - chr7.2:781309-781328 None:intergenic 45.0%
ACGTTCTTTGTTGGGCAGAT+GGG + chr7.2:780466-780485 MS.gene024426:CDS 45.0%
ACTCCACCTTCTTCTTCGTT+TGG - chr7.2:780504-780523 None:intergenic 45.0%
AGGATCTGAACAGCTTACCA+AGG + chr7.2:781868-781887 MS.gene024426:CDS 45.0%
CATTTGGAAGCAGTACTTCC+AGG + chr7.2:781609-781628 MS.gene024426:CDS 45.0%
CCAGAAGCAAAACACTTACC+GGG - chr7.2:780744-780763 None:intergenic 45.0%
CGAACCTCTAAATCATGTGG+AGG + chr7.2:781424-781443 MS.gene024426:CDS 45.0%
CTGATCAACCTAGCGATGAA+AGG + chr7.2:781360-781379 MS.gene024426:CDS 45.0%
GAACCAAACGAAGAAGAAGG+TGG + chr7.2:780498-780517 MS.gene024426:CDS 45.0%
GAGACCGGTTATTGCTGAAT+CGG + chr7.2:781316-781335 MS.gene024426:CDS 45.0%
GGACGAACCTCTAAATCATG+TGG + chr7.2:781421-781440 MS.gene024426:CDS 45.0%
TAAATCATGTGGAGGCAGAG+AGG + chr7.2:781432-781451 MS.gene024426:CDS 45.0%
TACGTTCTTTGTTGGGCAGA+TGG + chr7.2:780465-780484 MS.gene024426:CDS 45.0%
TAGAGTTTCTCTTCCGGATG+TGG + chr7.2:781676-781695 MS.gene024426:CDS 45.0%
TCTACTTGGGGAGTGAATCA+AGG + chr7.2:781158-781177 MS.gene024426:CDS 45.0%
TCTGAGTCTTGGCATAGAAG+AGG + chr7.2:780533-780552 MS.gene024426:CDS 45.0%
TCTGCCTCCACATGATTTAG+AGG - chr7.2:781431-781450 None:intergenic 45.0%
TGCTGCAATCAACTTGTCCT+TGG - chr7.2:781888-781907 None:intergenic 45.0%
! ACTTTTCCGGAGCAAATTCG+AGG + chr7.2:781282-781301 MS.gene024426:CDS 45.0%
! CTTTTCCGGAGCAAATTCGA+GGG + chr7.2:781283-781302 MS.gene024426:CDS 45.0%
!! ACATCTCTGTGTCGGTTACA+CGG - chr7.2:780674-780693 None:intergenic 45.0%
!! GAGAGTGGATTGATTGCTGT+TGG + chr7.2:780327-780346 MS.gene024426:CDS 45.0%
!! GGAACTTGTTGCTGATGTTG+AGG + chr7.2:781337-781356 MS.gene024426:CDS 45.0%
ACCAAGAACAGCTCCAACCA+TGG - chr7.2:780265-780284 None:intergenic 50.0%
ACCATGGTTGGAGCTGTTCT+TGG + chr7.2:780261-780280 MS.gene024426:CDS 50.0%
AGTACTTCCAGGGATGGATC+AGG + chr7.2:781620-781639 MS.gene024426:CDS 50.0%
CTAGCGATGAAAGGAGACCT+AGG + chr7.2:781369-781388 MS.gene024426:CDS 50.0%
CTAGGTCTCCTTTCATCGCT+AGG - chr7.2:781371-781390 None:intergenic 50.0%
GAAGGAAGCCTGCTAATGGA+AGG + chr7.2:781399-781418 MS.gene024426:CDS 50.0%
TGGGGAGTGAATCAAGGTCA+AGG + chr7.2:781164-781183 MS.gene024426:CDS 50.0%
TTGGCATCTGCCTTTGTGGT+TGG - chr7.2:781253-781272 None:intergenic 50.0%
! ATTGGCCCTCGAATTTGCTC+CGG - chr7.2:781291-781310 None:intergenic 50.0%
! CCCGGTAAGTGTTTTGCTTC+TGG + chr7.2:780741-780760 MS.gene024426:CDS 50.0%
! TGCATTGGCATCTGCCTTTG+TGG - chr7.2:781257-781276 None:intergenic 50.0%
!! TGTTCTTGGTGCTCGAGCTT+TGG + chr7.2:780275-780294 MS.gene024426:CDS 50.0%
ATGTGGAGGCAGAGAGGCAA+AGG + chr7.2:781438-781457 MS.gene024426:CDS 55.0%
GAAAGGAGACCTAGGAAGCG+CGG + chr7.2:781377-781396 MS.gene024426:CDS 55.0%
GGAAGCAGTACTTCCAGGGA+TGG + chr7.2:781614-781633 MS.gene024426:CDS 55.0%
GGTTCGTCCCTTCCATTAGC+AGG - chr7.2:781410-781429 None:intergenic 55.0%
! CGGGTCCACCATACCCTTTT+GGG - chr7.2:780725-780744 None:intergenic 55.0%
!! CCAAAAGGGTATGGTGGACC+CGG + chr7.2:780723-780742 MS.gene024426:CDS 55.0%
!! CTGTGTCGGTTACACGGTCA+AGG - chr7.2:780668-780687 None:intergenic 55.0%
CGAGTTGCCTGATCCATCCC+TGG - chr7.2:781630-781649 None:intergenic 60.0%
CGCGGAAGGAAGCCTGCTAA+TGG + chr7.2:781395-781414 MS.gene024426:CDS 60.0%
GAGGGCCAATTCTGCGAGAC+CGG + chr7.2:781301-781320 MS.gene024426:CDS 60.0%
! CCGGGTCCACCATACCCTTT+TGG - chr7.2:780726-780745 None:intergenic 60.0%
GGCTTCCTTCCGCGCTTCCT+AGG - chr7.2:781389-781408 None:intergenic 65.0%
! GGAGACCTAGGAAGCGCGGA+AGG + chr7.2:781381-781400 MS.gene024426:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 780219 781955 780219 ID=MS.gene024426
chr7.2 mRNA 780219 781955 780219 ID=MS.gene024426.t1;Parent=MS.gene024426
chr7.2 exon 780219 781955 780219 ID=MS.gene024426.t1.exon1;Parent=MS.gene024426.t1
chr7.2 CDS 780219 781955 780219 ID=cds.MS.gene024426.t1;Parent=MS.gene024426.t1
Gene Sequence

>MS.gene024426

ATGGGATCAATAAAGCGCATGATATGGAATGAAGAAGAGAAAACCATGGTTGGAGCTGTTCTTGGTGCTCGAGCTTTGGATTTTTTGATAAATAACTGTTTATCAAATGAGAGTGGATTGATTGCTGTTGGTGAAAGTGTTGATTCTCTTCAAAACAAACTCTCTGATCTTGTTGAAAATTCTTCATCCAATTTCAATTGGAATTACGCAATTTATTGGCGACTTTCTAAGTCAAAATCTGGTGATTACGTTCTTTGTTGGGCAGATGGGTGTTGCAGAGAACCAAACGAAGAAGAAGGTGGAGTTAGAGAAACTCTGAGTCTTGGCATAGAAGAGGAAAAAGAAGAAGAAGAAGAAGAAGGAATGAAACAAAGTATGAAGAAGTGGGTTTTGCAGAAGTTGCATAAAGCTTTTGGTGGTTCAGATGAAGACAATTACGCTTTTGGCCTTGACCGTGTAACCGACACAGAGATGTTCTTTTTAGCTTCTATGTACTTCTTTTTCCCAAAAGGGTATGGTGGACCCGGTAAGTGTTTTGCTTCTGGTAAACATTTGTGGTTCAAATCTGTTTCTGATTACTGCTTTCGTTCTTTCTTGGCTAAATCTGCTGGAATTCAAACTGTTGTTTTAGTACCTACTGATTTTGGTGTTGTTGAGTTGGGTTCTGTTAGATTGTTACCTGAAATCTTTGATTTGTTGAACAAAATCAAATCACTGTTTTCAATTTCAAAACCTGCCACACAATCATTATCATTGTATCCTAGTGTGGTCAATGAAGAAAGTACAGTTTCAAATGGTGTTCAAGTTCCAAAAGTTGTGTTGAATTTGAACAATGGTCGTACACATTTTAGGGAGAAACTTGCCATTAGGAAAATGGATAGTAATAATGTTAATAATAATAGCATTAACTTTCCTAGTGCTAGAAATGGTATTTGTGGTTCTACTTGGGGAGTGAATCAAGGTCAAGGTGAATTTTTTGCTTCTAATGGTGTGAGAAAGGATTTTAGGCTCAACAACAATAATAGCAACTACCAACCACAAAGGCAGATGCCAATGCAAATTGACTTTTCCGGAGCAAATTCGAGGGCCAATTCTGCGAGACCGGTTATTGCTGAATCGGAACTTGTTGCTGATGTTGAGGCTGATCAACCTAGCGATGAAAGGAGACCTAGGAAGCGCGGAAGGAAGCCTGCTAATGGAAGGGACGAACCTCTAAATCATGTGGAGGCAGAGAGGCAAAGGCGTGAGAAGCTTAATCAGCGTTTTTACGCCTTAAGAGCTGTTGTACCGAATATTTCCAAGATGGACAAGGCATCTTTACTTGGTGATGCTATTGCTTACATCAATGAGCTTCAAGCGAAACTCAAGATCATGGAGTCAGAGAGAGAATCATTTGGAAGCAGTACTTCCAGGGATGGATCAGGCAACTCGAGATCAGAAAATCGTCATCAGAATACTAGAGTTTCTCTTCCGGATGTGGATATTCAAGCTTCTCAAGATGAGGTAATAGTAAAGGTCAGCTGTTCTCTTGATACTCATCCTGTTTCGAAACTAATCGAAACATTCAAAGAGGCACAAATCAGTGTTGTAGAGTCGAAACTTGCTGCTGCAAATGATACTATCTACCATAATTTTGTAATTAAGTCAGAAGGATCTGAACAGCTTACCAAGGACAAGTTGATTGCAGCATTTGCCCGAGAATCCAACTCCTTACAAACCCAAACTTTGTCCATATAG
Protein sequence

>MS.gene024426.t1

MGSIKRMIWNEEEKTMVGAVLGARALDFLINNCLSNESGLIAVGESVDSLQNKLSDLVENSSSNFNWNYAIYWRLSKSKSGDYVLCWADGCCREPNEEEGGVRETLSLGIEEEKEEEEEEGMKQSMKKWVLQKLHKAFGGSDEDNYAFGLDRVTDTEMFFLASMYFFFPKGYGGPGKCFASGKHLWFKSVSDYCFRSFLAKSAGIQTVVLVPTDFGVVELGSVRLLPEIFDLLNKIKSLFSISKPATQSLSLYPSVVNEESTVSNGVQVPKVVLNLNNGRTHFREKLAIRKMDSNNVNNNSINFPSARNGICGSTWGVNQGQGEFFASNGVRKDFRLNNNNSNYQPQRQMPMQIDFSGANSRANSARPVIAESELVADVEADQPSDERRPRKRGRKPANGRDEPLNHVEAERQRREKLNQRFYALRAVVPNISKMDKASLLGDAIAYINELQAKLKIMESERESFGSSTSRDGSGNSRSENRHQNTRVSLPDVDIQASQDEVIVKVSCSLDTHPVSKLIETFKEAQISVVESKLAAANDTIYHNFVIKSEGSEQLTKDKLIAAFARESNSLQTQTLSI