Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025682.t1 | AES82159.1 | 94.5 | 145 | 3 | 2 | 1 | 140 | 1 | 145 | 1.00E-68 | 269.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025682.t1 | G7KSQ8 | 94.5 | 145 | 3 | 2 | 1 | 140 | 1 | 145 | 7.5e-69 | 269.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene07030 | MS.gene025682 | PPI |
| MS.gene28790 | MS.gene025682 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025682.t1 | MTR_7g109220 | 94.483 | 145 | 3 | 2 | 1 | 140 | 1 | 145 | 1.16e-95 | 272 |
| MS.gene025682.t1 | MTR_1g087775 | 45.000 | 140 | 52 | 8 | 6 | 125 | 10 | 144 | 4.40e-25 | 93.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025682.t1 | AT3G62650 | 43.151 | 146 | 69 | 5 | 4 | 140 | 1 | 141 | 5.17e-26 | 96.3 |
| MS.gene025682.t1 | AT3G62650 | 43.151 | 146 | 69 | 5 | 4 | 140 | 1 | 141 | 5.17e-26 | 96.3 |
| MS.gene025682.t1 | AT2G47485 | 41.935 | 124 | 67 | 2 | 10 | 129 | 4 | 126 | 3.16e-25 | 94.0 |
Find 41 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGATAATTTACCATCAAAAT+TGG | 0.121664 | 7.1:+4974311 | MS.gene025682:CDS |
| CCTCCATTTCTTCTTTGTTC+TGG | 0.201314 | 7.1:-4974043 | None:intergenic |
| AATGACCCCACATGTGGATT+TGG | 0.260607 | 7.1:+4974198 | MS.gene025682:CDS |
| ATCCACACCATCCATTTCTT+AGG | 0.343188 | 7.1:-4974108 | None:intergenic |
| ATGACCCCACATGTGGATTT+GGG | 0.354803 | 7.1:+4974199 | MS.gene025682:CDS |
| TAACTCGTTGGTTGTGGCTC+AGG | 0.369761 | 7.1:+4974272 | MS.gene025682:CDS |
| CAATTTCTTCTCCTAAGAAA+TGG | 0.376394 | 7.1:+4974097 | MS.gene025682:CDS |
| CCACCAGAACAAAGAAGAAA+TGG | 0.389999 | 7.1:+4974040 | MS.gene025682:CDS |
| CCATTTCTTCTTTGTTCTGG+TGG | 0.397013 | 7.1:-4974040 | None:intergenic |
| GGTTGTGGCTCAGGGACATT+TGG | 0.397366 | 7.1:+4974281 | MS.gene025682:CDS |
| TGTGAGGATGATGCTTGGTT+TGG | 0.423701 | 7.1:+4974143 | MS.gene025682:CDS |
| TTCTTAGGAGAAGAAATTGT+TGG | 0.424005 | 7.1:-4974093 | None:intergenic |
| TTATCGTATTCCTTAGGTTG+TGG | 0.435726 | 7.1:-4974228 | None:intergenic |
| AAGTACTGGAAGAGAAGAAA+GGG | 0.458738 | 7.1:+4973955 | MS.gene025682:CDS |
| GATAATTTACCATCAAAATT+GGG | 0.460538 | 7.1:+4974312 | MS.gene025682:CDS |
| AAAGTACTGGAAGAGAAGAA+AGG | 0.478666 | 7.1:+4973954 | MS.gene025682:CDS |
| AAGACGAACACGGTGAAGCT+TGG | 0.488739 | 7.1:+4974006 | MS.gene025682:CDS |
| TATCAAAGAATAAACAAGTC+AGG | 0.494739 | 7.1:+4973979 | MS.gene025682:CDS |
| TATGTATAACTCGTTGGTTG+TGG | 0.520612 | 7.1:+4974266 | MS.gene025682:CDS |
| TGGGAGGGCTCCACAACCTA+AGG | 0.524873 | 7.1:+4974218 | MS.gene025682:CDS |
| TGTTCATATGTATAACTCGT+TGG | 0.532049 | 7.1:+4974260 | MS.gene025682:CDS |
| AACTCGTTGGTTGTGGCTCA+GGG | 0.548116 | 7.1:+4974273 | MS.gene025682:CDS |
| ACGAACACGGTGAAGCTTGG+AGG | 0.557850 | 7.1:+4974009 | MS.gene025682:CDS |
| TCCTATGTGAGGATGATGCT+TGG | 0.566278 | 7.1:+4974138 | MS.gene025682:CDS |
| GCTTGGTTTGGCTAACTCGA+AGG | 0.569641 | 7.1:+4974155 | MS.gene025682:CDS |
| GCCCTCCCAAATCCACATGT+GGG | 0.571246 | 7.1:-4974204 | None:intergenic |
| AACACGGTGAAGCTTGGAGG+AGG | 0.574678 | 7.1:+4974012 | MS.gene025682:CDS |
| TTTGATGGTAAATTATCGAG+TGG | 0.574914 | 7.1:-4974306 | None:intergenic |
| CTCCTAAGAAATGGATGGTG+TGG | 0.579452 | 7.1:+4974106 | MS.gene025682:CDS |
| TTCTTCTCCTAAGAAATGGA+TGG | 0.584234 | 7.1:+4974101 | MS.gene025682:CDS |
| CCCCACATGTGGATTTGGGA+GGG | 0.593058 | 7.1:+4974203 | MS.gene025682:CDS |
| AGCTTCAATGACCCCACATG+TGG | 0.606064 | 7.1:+4974192 | MS.gene025682:CDS |
| AGCCCTCCCAAATCCACATG+TGG | 0.606097 | 7.1:-4974205 | None:intergenic |
| ACCCCACATGTGGATTTGGG+AGG | 0.612193 | 7.1:+4974202 | MS.gene025682:CDS |
| AAAGAATAAACAAGTCAGGT+CGG | 0.632985 | 7.1:+4973983 | MS.gene025682:CDS |
| CCATCATGGAATGCATGCAA+AGG | 0.638361 | 7.1:+4973923 | None:intergenic |
| CCCTCCCAAATCCACATGTG+GGG | 0.655405 | 7.1:-4974203 | None:intergenic |
| CCAGAACAAAGAAGAAATGG+AGG | 0.660658 | 7.1:+4974043 | MS.gene025682:CDS |
| ACCAAGCATCATCCTCACAT+AGG | 0.675038 | 7.1:-4974139 | None:intergenic |
| GTCAGGTCGGAAGACGAACA+CGG | 0.682033 | 7.1:+4973996 | MS.gene025682:CDS |
| GGATGAGAGACTCCTATGTG+AGG | 0.755000 | 7.1:+4974127 | MS.gene025682:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAATTTACCATCAAAATT+GGG | + | chr7.1:4974312-4974331 | MS.gene025682:CDS | 20.0% |
| !!! | ATTGTTGGAAATTTTATCTT+AGG | - | chr7.1:4974081-4974100 | None:intergenic | 20.0% |
| !!! | ATTTTATTATCGTATTCCTT+AGG | - | chr7.1:4974237-4974256 | None:intergenic | 20.0% |
| ! | CGATAATTTACCATCAAAAT+TGG | + | chr7.1:4974311-4974330 | MS.gene025682:CDS | 25.0% |
| ! | TATCAAAGAATAAACAAGTC+AGG | + | chr7.1:4973979-4973998 | MS.gene025682:CDS | 25.0% |
| AAAGAATAAACAAGTCAGGT+CGG | + | chr7.1:4973983-4974002 | MS.gene025682:CDS | 30.0% | |
| CAATTTCTTCTCCTAAGAAA+TGG | + | chr7.1:4974097-4974116 | MS.gene025682:CDS | 30.0% | |
| TGTTCATATGTATAACTCGT+TGG | + | chr7.1:4974260-4974279 | MS.gene025682:CDS | 30.0% | |
| TTCTTAGGAGAAGAAATTGT+TGG | - | chr7.1:4974096-4974115 | None:intergenic | 30.0% | |
| ! | AAAGGAGTTTGAAAAAGTAC+TGG | + | chr7.1:4973941-4973960 | MS.gene025682:CDS | 30.0% |
| ! | TTTGATGGTAAATTATCGAG+TGG | - | chr7.1:4974309-4974328 | None:intergenic | 30.0% |
| TTCTTCTCCTAAGAAATGGA+TGG | + | chr7.1:4974101-4974120 | MS.gene025682:CDS | 35.0% | |
| ! | TATGTATAACTCGTTGGTTG+TGG | + | chr7.1:4974266-4974285 | MS.gene025682:CDS | 35.0% |
| ! | TTATCGTATTCCTTAGGTTG+TGG | - | chr7.1:4974231-4974250 | None:intergenic | 35.0% |
| !! | AAAGTACTGGAAGAGAAGAA+AGG | + | chr7.1:4973954-4973973 | MS.gene025682:CDS | 35.0% |
| !! | AAGTACTGGAAGAGAAGAAA+GGG | + | chr7.1:4973955-4973974 | MS.gene025682:CDS | 35.0% |
| ATCCACACCATCCATTTCTT+AGG | - | chr7.1:4974111-4974130 | None:intergenic | 40.0% | |
| CCACCAGAACAAAGAAGAAA+TGG | + | chr7.1:4974040-4974059 | MS.gene025682:CDS | 40.0% | |
| CCAGAACAAAGAAGAAATGG+AGG | + | chr7.1:4974043-4974062 | MS.gene025682:CDS | 40.0% | |
| CCATTTCTTCTTTGTTCTGG+TGG | - | chr7.1:4974043-4974062 | None:intergenic | 40.0% | |
| CCTCCATTTCTTCTTTGTTC+TGG | - | chr7.1:4974046-4974065 | None:intergenic | 40.0% | |
| ! | GTTTCTGAGCCCAATTTTGA+TGG | - | chr7.1:4974324-4974343 | None:intergenic | 40.0% |
| AATGACCCCACATGTGGATT+TGG | + | chr7.1:4974198-4974217 | MS.gene025682:CDS | 45.0% | |
| ACCAAGCATCATCCTCACAT+AGG | - | chr7.1:4974142-4974161 | None:intergenic | 45.0% | |
| ATGACCCCACATGTGGATTT+GGG | + | chr7.1:4974199-4974218 | MS.gene025682:CDS | 45.0% | |
| CTCCTAAGAAATGGATGGTG+TGG | + | chr7.1:4974106-4974125 | MS.gene025682:CDS | 45.0% | |
| TCCTATGTGAGGATGATGCT+TGG | + | chr7.1:4974138-4974157 | MS.gene025682:CDS | 45.0% | |
| TGTGAGGATGATGCTTGGTT+TGG | + | chr7.1:4974143-4974162 | MS.gene025682:CDS | 45.0% | |
| AAGACGAACACGGTGAAGCT+TGG | + | chr7.1:4974006-4974025 | MS.gene025682:CDS | 50.0% | |
| AGCTTCAATGACCCCACATG+TGG | + | chr7.1:4974192-4974211 | MS.gene025682:CDS | 50.0% | |
| GGATGAGAGACTCCTATGTG+AGG | + | chr7.1:4974127-4974146 | MS.gene025682:CDS | 50.0% | |
| ! | AACTCGTTGGTTGTGGCTCA+GGG | + | chr7.1:4974273-4974292 | MS.gene025682:CDS | 50.0% |
| ! | TAACTCGTTGGTTGTGGCTC+AGG | + | chr7.1:4974272-4974291 | MS.gene025682:CDS | 50.0% |
| !! | GCTTGGTTTGGCTAACTCGA+AGG | + | chr7.1:4974155-4974174 | MS.gene025682:CDS | 50.0% |
| AACACGGTGAAGCTTGGAGG+AGG | + | chr7.1:4974012-4974031 | MS.gene025682:CDS | 55.0% | |
| ACCCCACATGTGGATTTGGG+AGG | + | chr7.1:4974202-4974221 | MS.gene025682:CDS | 55.0% | |
| ACGAACACGGTGAAGCTTGG+AGG | + | chr7.1:4974009-4974028 | MS.gene025682:CDS | 55.0% | |
| AGCCCTCCCAAATCCACATG+TGG | - | chr7.1:4974208-4974227 | None:intergenic | 55.0% | |
| CCCCACATGTGGATTTGGGA+GGG | + | chr7.1:4974203-4974222 | MS.gene025682:CDS | 55.0% | |
| CCCTCCCAAATCCACATGTG+GGG | - | chr7.1:4974206-4974225 | None:intergenic | 55.0% | |
| GCCCTCCCAAATCCACATGT+GGG | - | chr7.1:4974207-4974226 | None:intergenic | 55.0% | |
| GGTTGTGGCTCAGGGACATT+TGG | + | chr7.1:4974281-4974300 | MS.gene025682:CDS | 55.0% | |
| GTCAGGTCGGAAGACGAACA+CGG | + | chr7.1:4973996-4974015 | MS.gene025682:CDS | 55.0% | |
| TGGGAGGGCTCCACAACCTA+AGG | + | chr7.1:4974218-4974237 | MS.gene025682:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 4973928 | 4974350 | 4973928 | ID=MS.gene025682 |
| chr7.1 | mRNA | 4973928 | 4974350 | 4973928 | ID=MS.gene025682.t1;Parent=MS.gene025682 |
| chr7.1 | exon | 4973928 | 4974350 | 4973928 | ID=MS.gene025682.t1.exon1;Parent=MS.gene025682.t1 |
| chr7.1 | CDS | 4973928 | 4974350 | 4973928 | ID=cds.MS.gene025682.t1;Parent=MS.gene025682.t1 |
| Gene Sequence |
| Protein sequence |
