AlfalfaGEDB | MS.gene07030

M. sativa cultivar XinJiangDaYe / MS.gene07030

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07030.t1	AES82159.1	94.5	145	3	2	1	140	1	145	1.00E-68	269.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07030.t1	G7KSQ8	94.5	145	3	2	1	140	1	145	7.5e-69	269.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene07030	0.810189	1.30E-50	-1.69E-46
MS.gene049232	MS.gene07030	0.815544	8.76E-52	-1.69E-46
MS.gene049285	MS.gene07030	0.817799	2.73E-52	-1.69E-46
MS.gene049320	MS.gene07030	0.813689	2.25E-51	-1.69E-46
MS.gene049828	MS.gene07030	0.803679	3.11E-49	-1.69E-46
MS.gene050163	MS.gene07030	0.816125	6.50E-52	-1.69E-46
MS.gene050165	MS.gene07030	0.811992	5.30E-51	-1.69E-46
MS.gene050216	MS.gene07030	0.826228	3.04E-54	-1.69E-46
MS.gene050333	MS.gene07030	0.817415	3.34E-52	-1.69E-46
MS.gene050444	MS.gene07030	0.815647	8.30E-52	-1.69E-46
MS.gene050504	MS.gene07030	0.813412	2.59E-51	-1.69E-46
MS.gene050518	MS.gene07030	0.825462	4.63E-54	-1.69E-46
MS.gene050612	MS.gene07030	0.83083	2.35E-55	-1.69E-46
MS.gene050662	MS.gene07030	0.804047	2.61E-49	-1.69E-46
MS.gene050675	MS.gene07030	0.809711	1.65E-50	-1.69E-46
MS.gene050871	MS.gene07030	0.800559	1.36E-48	-1.69E-46
MS.gene051228	MS.gene07030	0.828327	9.56E-55	-1.69E-46
MS.gene051268	MS.gene07030	0.812075	5.09E-51	-1.69E-46
MS.gene051949	MS.gene07030	0.80026	1.57E-48	-1.69E-46
MS.gene053916	MS.gene07030	0.825206	5.32E-54	-1.69E-46
MS.gene054595	MS.gene07030	0.811351	7.31E-51	-1.69E-46
MS.gene054642	MS.gene07030	0.826551	2.55E-54	-1.69E-46
MS.gene054954	MS.gene07030	0.809843	1.55E-50	-1.69E-46
MS.gene055748	MS.gene07030	0.803032	4.23E-49	-1.69E-46
MS.gene055750	MS.gene07030	0.810897	9.17E-51	-1.69E-46
MS.gene055751	MS.gene07030	0.813118	3.01E-51	-1.69E-46
MS.gene055776	MS.gene07030	0.802223	6.21E-49	-1.69E-46
MS.gene05592	MS.gene07030	0.812521	4.07E-51	-1.69E-46
MS.gene056374	MS.gene07030	0.804615	1.98E-49	-1.69E-46
MS.gene056422	MS.gene07030	0.808279	3.35E-50	-1.69E-46
MS.gene056510	MS.gene07030	0.822429	2.38E-53	-1.69E-46
MS.gene056657	MS.gene07030	0.825575	4.35E-54	-1.69E-46
MS.gene057537	MS.gene07030	0.801364	9.32E-49	-1.69E-46
MS.gene057926	MS.gene07030	0.825751	3.95E-54	-1.69E-46
MS.gene058459	MS.gene07030	0.830562	2.74E-55	-1.69E-46
MS.gene058773	MS.gene07030	0.816048	6.76E-52	-1.69E-46
MS.gene05913	MS.gene07030	0.822632	2.14E-53	-1.69E-46
MS.gene059367	MS.gene07030	0.831366	1.74E-55	-1.69E-46
MS.gene059711	MS.gene07030	0.805103	1.57E-49	-1.69E-46
MS.gene059718	MS.gene07030	0.801751	7.77E-49	-1.69E-46
MS.gene059865	MS.gene07030	0.822154	2.76E-53	-1.69E-46
MS.gene060818	MS.gene07030	0.802434	5.62E-49	-1.69E-46
MS.gene061041	MS.gene07030	0.803652	3.15E-49	-1.69E-46
MS.gene061134	MS.gene07030	0.806959	6.39E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene07030	MS.gene025682	PPI
MS.gene07030	MS.gene28790	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07030.t1	MTR_7g109220	94.483	145	3	2	1	140	1	145	1.16e-95	272
MS.gene07030.t1	MTR_1g087775	45.000	140	52	8	6	125	10	144	4.40e-25	93.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07030.t1	AT3G62650	43.151	146	69	5	4	140	1	141	5.17e-26	96.3
MS.gene07030.t1	AT3G62650	43.151	146	69	5	4	140	1	141	5.17e-26	96.3
MS.gene07030.t1	AT2G47485	41.935	124	67	2	10	129	4	126	3.16e-25	94.0

Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGATAATTTACCATCAAAAT+TGG	0.121664	7.2:-5421767	MS.gene07030:CDS
CCTCCATTTCTTCTTTGTTC+TGG	0.201314	7.2:+5422035	None:intergenic
AATGACCCCACATGTGGATT+TGG	0.260607	7.2:-5421880	MS.gene07030:CDS
ATCCACACCATCCATTTCTT+AGG	0.343188	7.2:+5421970	None:intergenic
ATGACCCCACATGTGGATTT+GGG	0.354803	7.2:-5421879	MS.gene07030:CDS
TAACTCGTTGGTTGTGGCTC+AGG	0.369761	7.2:-5421806	MS.gene07030:CDS
CAATTTCTTCTCCTAAGAAA+TGG	0.376394	7.2:-5421981	MS.gene07030:CDS
CCACCAGAACAAAGAAGAAA+TGG	0.389999	7.2:-5422038	MS.gene07030:CDS
CCATTTCTTCTTTGTTCTGG+TGG	0.397013	7.2:+5422038	None:intergenic
GGTTGTGGCTCAGGGACATT+TGG	0.397366	7.2:-5421797	MS.gene07030:CDS
TGTGAGGATGATGCTTGGTT+TGG	0.423701	7.2:-5421935	MS.gene07030:CDS
TTCTTAGGAGAAGAAATTGT+TGG	0.424005	7.2:+5421985	None:intergenic
TTATCGTATTCCTTAGGTTG+TGG	0.435726	7.2:+5421850	None:intergenic
AAGTACTGGAAGAGAAGAAA+GGG	0.458738	7.2:-5422123	MS.gene07030:CDS
GATAATTTACCATCAAAATT+GGG	0.460538	7.2:-5421766	MS.gene07030:CDS
AAAGTACTGGAAGAGAAGAA+AGG	0.478666	7.2:-5422124	MS.gene07030:CDS
AAGACGAACACGGTGAAGCT+TGG	0.488739	7.2:-5422072	MS.gene07030:CDS
TATCAAAGAATAAACAAGTC+AGG	0.494739	7.2:-5422099	MS.gene07030:CDS
TATGTATAACTCGTTGGTTG+TGG	0.520612	7.2:-5421812	MS.gene07030:CDS
TGGGAGGGCTCCACAACCTA+AGG	0.524873	7.2:-5421860	MS.gene07030:CDS
TGTTCATATGTATAACTCGT+TGG	0.532049	7.2:-5421818	MS.gene07030:CDS
AACTCGTTGGTTGTGGCTCA+GGG	0.548116	7.2:-5421805	MS.gene07030:CDS
ACGAACACGGTGAAGCTTGG+AGG	0.557850	7.2:-5422069	MS.gene07030:CDS
TCCTATGTGAGGATGATGCT+TGG	0.566278	7.2:-5421940	MS.gene07030:CDS
GCTTGGTTTGGCTAACTCGA+AGG	0.569641	7.2:-5421923	MS.gene07030:CDS
GCCCTCCCAAATCCACATGT+GGG	0.571246	7.2:+5421874	None:intergenic
AACACGGTGAAGCTTGGAGG+AGG	0.574678	7.2:-5422066	MS.gene07030:CDS
TTTGATGGTAAATTATCGAG+TGG	0.574914	7.2:+5421772	None:intergenic
CTCCTAAGAAATGGATGGTG+TGG	0.579452	7.2:-5421972	MS.gene07030:CDS
TTCTTCTCCTAAGAAATGGA+TGG	0.584234	7.2:-5421977	MS.gene07030:CDS
CCCCACATGTGGATTTGGGA+GGG	0.593058	7.2:-5421875	MS.gene07030:CDS
AGCTTCAATGACCCCACATG+TGG	0.606064	7.2:-5421886	MS.gene07030:CDS
AGCCCTCCCAAATCCACATG+TGG	0.606097	7.2:+5421873	None:intergenic
ACCCCACATGTGGATTTGGG+AGG	0.612193	7.2:-5421876	MS.gene07030:CDS
AAAGAATAAACAAGTCAGGT+CGG	0.632985	7.2:-5422095	MS.gene07030:CDS
CCATCATGGAATGCATGCAA+AGG	0.638361	7.2:-5422155	None:intergenic
CCCTCCCAAATCCACATGTG+GGG	0.655405	7.2:+5421875	None:intergenic
CCAGAACAAAGAAGAAATGG+AGG	0.660658	7.2:-5422035	MS.gene07030:CDS
ACCAAGCATCATCCTCACAT+AGG	0.675038	7.2:+5421939	None:intergenic
GTCAGGTCGGAAGACGAACA+CGG	0.682033	7.2:-5422082	MS.gene07030:CDS
GGATGAGAGACTCCTATGTG+AGG	0.755000	7.2:-5421951	MS.gene07030:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATAATTTACCATCAAAATT+GGG	-	chr7.2:5422134-5422153	MS.gene07030:CDS	20.0%
!!!	ATTGTTGGAAATTTTATCTT+AGG	+	chr7.2:5421903-5421922	None:intergenic	20.0%
!!!	ATTTTATTATCGTATTCCTT+AGG	+	chr7.2:5422059-5422078	None:intergenic	20.0%
!	CGATAATTTACCATCAAAAT+TGG	-	chr7.2:5422133-5422152	MS.gene07030:CDS	25.0%
!	TATCAAAGAATAAACAAGTC+AGG	-	chr7.2:5421801-5421820	MS.gene07030:CDS	25.0%
	AAAGAATAAACAAGTCAGGT+CGG	-	chr7.2:5421805-5421824	MS.gene07030:CDS	30.0%
	CAATTTCTTCTCCTAAGAAA+TGG	-	chr7.2:5421919-5421938	MS.gene07030:CDS	30.0%
	TGTTCATATGTATAACTCGT+TGG	-	chr7.2:5422082-5422101	MS.gene07030:CDS	30.0%
	TTCTTAGGAGAAGAAATTGT+TGG	+	chr7.2:5421918-5421937	None:intergenic	30.0%
!	AAAGGAGTTTGAAAAAGTAC+TGG	-	chr7.2:5421763-5421782	MS.gene07030:CDS	30.0%
!	TTTGATGGTAAATTATCGAG+TGG	+	chr7.2:5422131-5422150	None:intergenic	30.0%
	TTCTTCTCCTAAGAAATGGA+TGG	-	chr7.2:5421923-5421942	MS.gene07030:CDS	35.0%
!	TATGTATAACTCGTTGGTTG+TGG	-	chr7.2:5422088-5422107	MS.gene07030:CDS	35.0%
!	TTATCGTATTCCTTAGGTTG+TGG	+	chr7.2:5422053-5422072	None:intergenic	35.0%
!!	AAAGTACTGGAAGAGAAGAA+AGG	-	chr7.2:5421776-5421795	MS.gene07030:CDS	35.0%
!!	AAGTACTGGAAGAGAAGAAA+GGG	-	chr7.2:5421777-5421796	MS.gene07030:CDS	35.0%
	ATCCACACCATCCATTTCTT+AGG	+	chr7.2:5421933-5421952	None:intergenic	40.0%
	CCACCAGAACAAAGAAGAAA+TGG	-	chr7.2:5421862-5421881	MS.gene07030:CDS	40.0%
	CCAGAACAAAGAAGAAATGG+AGG	-	chr7.2:5421865-5421884	MS.gene07030:CDS	40.0%
	CCATTTCTTCTTTGTTCTGG+TGG	+	chr7.2:5421865-5421884	None:intergenic	40.0%
	CCTCCATTTCTTCTTTGTTC+TGG	+	chr7.2:5421868-5421887	None:intergenic	40.0%
!	GTTTCTGAGCCCAATTTTGA+TGG	+	chr7.2:5422146-5422165	None:intergenic	40.0%
	AATGACCCCACATGTGGATT+TGG	-	chr7.2:5422020-5422039	MS.gene07030:CDS	45.0%
	ACCAAGCATCATCCTCACAT+AGG	+	chr7.2:5421964-5421983	None:intergenic	45.0%
	ATGACCCCACATGTGGATTT+GGG	-	chr7.2:5422021-5422040	MS.gene07030:CDS	45.0%
	CTCCTAAGAAATGGATGGTG+TGG	-	chr7.2:5421928-5421947	MS.gene07030:CDS	45.0%
	TCCTATGTGAGGATGATGCT+TGG	-	chr7.2:5421960-5421979	MS.gene07030:CDS	45.0%
	TGTGAGGATGATGCTTGGTT+TGG	-	chr7.2:5421965-5421984	MS.gene07030:CDS	45.0%
	AAGACGAACACGGTGAAGCT+TGG	-	chr7.2:5421828-5421847	MS.gene07030:CDS	50.0%
	AGCTTCAATGACCCCACATG+TGG	-	chr7.2:5422014-5422033	MS.gene07030:CDS	50.0%
	GGATGAGAGACTCCTATGTG+AGG	-	chr7.2:5421949-5421968	MS.gene07030:CDS	50.0%
!	AACTCGTTGGTTGTGGCTCA+GGG	-	chr7.2:5422095-5422114	MS.gene07030:CDS	50.0%
!	TAACTCGTTGGTTGTGGCTC+AGG	-	chr7.2:5422094-5422113	MS.gene07030:CDS	50.0%
!!	GCTTGGTTTGGCTAACTCGA+AGG	-	chr7.2:5421977-5421996	MS.gene07030:CDS	50.0%
	AACACGGTGAAGCTTGGAGG+AGG	-	chr7.2:5421834-5421853	MS.gene07030:CDS	55.0%
	ACCCCACATGTGGATTTGGG+AGG	-	chr7.2:5422024-5422043	MS.gene07030:CDS	55.0%
	ACGAACACGGTGAAGCTTGG+AGG	-	chr7.2:5421831-5421850	MS.gene07030:CDS	55.0%
	AGCCCTCCCAAATCCACATG+TGG	+	chr7.2:5422030-5422049	None:intergenic	55.0%
	CCCCACATGTGGATTTGGGA+GGG	-	chr7.2:5422025-5422044	MS.gene07030:CDS	55.0%
	CCCTCCCAAATCCACATGTG+GGG	+	chr7.2:5422028-5422047	None:intergenic	55.0%
	GCCCTCCCAAATCCACATGT+GGG	+	chr7.2:5422029-5422048	None:intergenic	55.0%
	GGTTGTGGCTCAGGGACATT+TGG	-	chr7.2:5422103-5422122	MS.gene07030:CDS	55.0%
	GTCAGGTCGGAAGACGAACA+CGG	-	chr7.2:5421818-5421837	MS.gene07030:CDS	55.0%
	TGGGAGGGCTCCACAACCTA+AGG	-	chr7.2:5422040-5422059	MS.gene07030:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	5421750	5422172	5421750	ID=MS.gene07030
chr7.2	mRNA	5421750	5422172	5421750	ID=MS.gene07030.t1;Parent=MS.gene07030
chr7.2	exon	5421750	5422172	5421750	ID=MS.gene07030.t1.exon1;Parent=MS.gene07030.t1
chr7.2	CDS	5421750	5422172	5421750	ID=cds.MS.gene07030.t1;Parent=MS.gene07030.t1

Gene Sequence

>MS.gene07030

ATGGAATGCATGCAAAGGAGTTTGAAAAAGTACTGGAAGAGAAGAAAGGGATATCAAAGAATAAACAAGTCAGGTCGGAAGACGAACACGGTGAAGCTTGGAGGAGGAGCAACCACCAGAACAAAGAAGAAATGGAGGATCAAAATTTCTCCTAAGATAAAATTTCCAACAATTTCTTCTCCTAAGAAATGGATGGTGTGGATGAGAGACTCCTATGTGAGGATGATGCTTGGTTTGGCTAACTCGAAGGTAATGAATTTGACTAGCTTCAATGACCCCACATGTGGATTTGGGAGGGCTCCACAACCTAAGGAATACGATAATAAAATGCTTGTTCATATGTATAACTCGTTGGTTGTGGCTCAGGGACATTTGGTTCCACTCGATAATTTACCATCAAAATTGGGCTCAGAAACAGCTTGA

Protein sequence

>MS.gene07030.t1

MECMQRSLKKYWKRRKGYQRINKSGRKTNTVKLGGGATTRTKKKWRIKISPKIKFPTISSPKKWMVWMRDSYVRMMLGLANSKVMNLTSFNDPTCGFGRAPQPKEYDNKMLVHMYNSLVVAQGHLVPLDNLPSKLGSETA