AlfalfaGEDB | MS.gene28790

M. sativa cultivar XinJiangDaYe / MS.gene28790

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28790.t1	AES82159.1	93.8	145	4	2	1	140	1	145	4.00E-68	267.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28790.t1	G7KSQ8	93.8	145	4	2	1	140	1	145	2.9e-68	267.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene28790	0.81116	8.04E-51	-1.69E-46
MS.gene049232	MS.gene28790	0.833233	6.00E-56	-1.69E-46
MS.gene049369	MS.gene28790	0.808051	3.74E-50	-1.69E-46
MS.gene049396	MS.gene28790	0.829537	4.87E-55	-1.69E-46
MS.gene050216	MS.gene28790	0.814043	1.88E-51	-1.69E-46
MS.gene050499	MS.gene28790	0.818021	2.44E-52	-1.69E-46
MS.gene050504	MS.gene28790	0.801964	7.02E-49	-1.69E-46
MS.gene050518	MS.gene28790	0.807935	3.96E-50	-1.69E-46
MS.gene050520	MS.gene28790	0.805945	1.04E-49	-1.69E-46
MS.gene050612	MS.gene28790	0.804181	2.44E-49	-1.69E-46
MS.gene050662	MS.gene28790	0.805925	1.06E-49	-1.69E-46
MS.gene050675	MS.gene28790	0.804065	2.58E-49	-1.69E-46
MS.gene050772	MS.gene28790	0.803945	2.74E-49	-1.69E-46
MS.gene05080	MS.gene28790	0.819276	1.26E-52	-1.69E-46
MS.gene050871	MS.gene28790	0.804519	2.08E-49	-1.69E-46
MS.gene051509	MS.gene28790	0.836088	1.15E-56	-1.69E-46
MS.gene052164	MS.gene28790	0.801706	7.93E-49	-1.69E-46
MS.gene053574	MS.gene28790	0.833063	6.61E-56	-1.69E-46
MS.gene053626	MS.gene28790	0.830234	3.29E-55	-1.69E-46
MS.gene054595	MS.gene28790	0.80506	1.60E-49	-1.69E-46
MS.gene054642	MS.gene28790	0.811752	5.98E-51	-1.69E-46
MS.gene056166	MS.gene28790	0.803218	3.87E-49	-1.69E-46
MS.gene056422	MS.gene28790	0.848894	4.58E-60	-1.69E-46
MS.gene056510	MS.gene28790	0.808755	2.65E-50	-1.69E-46
MS.gene056657	MS.gene28790	0.803313	3.70E-49	-1.69E-46
MS.gene057086	MS.gene28790	0.804683	1.92E-49	-1.69E-46
MS.gene057537	MS.gene28790	0.804234	2.38E-49	-1.69E-46
MS.gene05787	MS.gene28790	0.805815	1.11E-49	-1.69E-46
MS.gene058130	MS.gene28790	0.812418	4.28E-51	-1.69E-46
MS.gene058459	MS.gene28790	0.83381	4.31E-56	-1.69E-46
MS.gene05913	MS.gene28790	0.808883	2.49E-50	-1.69E-46
MS.gene059367	MS.gene28790	0.838599	2.61E-57	-1.69E-46
MS.gene059368	MS.gene28790	0.812999	3.19E-51	-1.69E-46
MS.gene059711	MS.gene28790	0.826643	2.42E-54	-1.69E-46
MS.gene059718	MS.gene28790	0.801081	1.07E-48	-1.69E-46
MS.gene059865	MS.gene28790	0.836294	1.02E-56	-1.69E-46
MS.gene060482	MS.gene28790	0.815593	8.54E-52	-1.69E-46
MS.gene060778	MS.gene28790	0.809338	1.99E-50	-1.69E-46
MS.gene061041	MS.gene28790	0.809573	1.77E-50	-1.69E-46
MS.gene061134	MS.gene28790	0.829644	4.59E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene07030	MS.gene28790	PPI
MS.gene28790	MS.gene025682	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28790.t1	MTR_7g109220	93.793	145	4	2	1	140	1	145	4.20e-95	271
MS.gene28790.t1	MTR_1g087775	44.286	140	53	8	6	125	10	144	1.76e-24	92.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28790.t1	AT3G62650	43.151	146	69	5	4	140	1	141	2.82e-26	97.1
MS.gene28790.t1	AT3G62650	43.151	146	69	5	4	140	1	141	2.82e-26	97.1
MS.gene28790.t1	AT2G47485	40.323	124	69	2	10	129	4	126	7.12e-25	92.8

Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGATAATTTACCATCAAAAT+TGG	0.121664	7.4:+6101890	MS.gene28790:CDS
CCTCCATTTCTTCTTTGTTC+TGG	0.201314	7.4:-6101622	None:intergenic
AATGACCCCACATGTGGATT+TGG	0.260607	7.4:+6101777	MS.gene28790:CDS
ATCCACACCATCCATTTCTT+AGG	0.343188	7.4:-6101687	None:intergenic
ATGACCCCACATGTGGATTT+GGG	0.354803	7.4:+6101778	MS.gene28790:CDS
TAACTCGTTGGTTGTGGCTC+AGG	0.369761	7.4:+6101851	MS.gene28790:CDS
CAATTTCTTCTCCTAAGAAA+TGG	0.376394	7.4:+6101676	MS.gene28790:CDS
CCACCAGAACAAAGAAGAAA+TGG	0.389999	7.4:+6101619	MS.gene28790:CDS
CCATTTCTTCTTTGTTCTGG+TGG	0.397013	7.4:-6101619	None:intergenic
GGTTGTGGCTCAGGGACATT+TGG	0.397366	7.4:+6101860	MS.gene28790:CDS
TGTGAGGATGATGCTTGGTT+TGG	0.423701	7.4:+6101722	MS.gene28790:CDS
TTCTTAGGAGAAGAAATTGT+TGG	0.424005	7.4:-6101672	None:intergenic
TTATCGTATTCCTTAGGTTG+TGG	0.435726	7.4:-6101807	None:intergenic
AAGTACTGGAAGAGAAGAAA+GGG	0.458738	7.4:+6101534	MS.gene28790:CDS
GATAATTTACCATCAAAATT+GGG	0.460538	7.4:+6101891	MS.gene28790:CDS
AAGACGAACACGGCGAAGCT+TGG	0.466577	7.4:+6101585	MS.gene28790:CDS
AAAGTACTGGAAGAGAAGAA+AGG	0.478666	7.4:+6101533	MS.gene28790:CDS
TATCAAAGAATAAACAAGTC+AGG	0.494739	7.4:+6101558	MS.gene28790:CDS
TATGTATAACTCGTTGGTTG+TGG	0.520612	7.4:+6101845	MS.gene28790:CDS
TGGGAGGGCTCCACAACCTA+AGG	0.524873	7.4:+6101797	MS.gene28790:CDS
TGTTCATATGTATAACTCGT+TGG	0.532049	7.4:+6101839	MS.gene28790:CDS
ACGAACACGGCGAAGCTTGG+AGG	0.537542	7.4:+6101588	MS.gene28790:CDS
AACTCGTTGGTTGTGGCTCA+GGG	0.548116	7.4:+6101852	MS.gene28790:CDS
AACACGGCGAAGCTTGGAGG+AGG	0.550769	7.4:+6101591	MS.gene28790:CDS
TCCTATGTGAGGATGATGCT+TGG	0.566278	7.4:+6101717	MS.gene28790:CDS
GCTTGGTTTGGCTAACTCGA+AGG	0.569641	7.4:+6101734	MS.gene28790:CDS
GCCCTCCCAAATCCACATGT+GGG	0.571246	7.4:-6101783	None:intergenic
TTTGATGGTAAATTATCGAG+TGG	0.574914	7.4:-6101885	None:intergenic
CTCCTAAGAAATGGATGGTG+TGG	0.579452	7.4:+6101685	MS.gene28790:CDS
TTCTTCTCCTAAGAAATGGA+TGG	0.584234	7.4:+6101680	MS.gene28790:CDS
CCCCACATGTGGATTTGGGA+GGG	0.593058	7.4:+6101782	MS.gene28790:CDS
AGCTTCAATGACCCCACATG+TGG	0.606064	7.4:+6101771	MS.gene28790:CDS
AGCCCTCCCAAATCCACATG+TGG	0.606097	7.4:-6101784	None:intergenic
ACCCCACATGTGGATTTGGG+AGG	0.612193	7.4:+6101781	MS.gene28790:CDS
AAAGAATAAACAAGTCAGGT+CGG	0.632985	7.4:+6101562	MS.gene28790:CDS
CCATCATGGAATGCATGCAA+AGG	0.638361	7.4:+6101502	None:intergenic
CCCTCCCAAATCCACATGTG+GGG	0.655405	7.4:-6101782	None:intergenic
CCAGAACAAAGAAGAAATGG+AGG	0.660658	7.4:+6101622	MS.gene28790:CDS
GTCAGGTCGGAAGACGAACA+CGG	0.666994	7.4:+6101575	MS.gene28790:CDS
ACCAAGCATCATCCTCACAT+AGG	0.675038	7.4:-6101718	None:intergenic
GGATGAGAGACTCCTATGTG+AGG	0.755000	7.4:+6101706	MS.gene28790:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATAATTTACCATCAAAATT+GGG	+	chr7.4:6101891-6101910	MS.gene28790:CDS	20.0%
!!!	ATTGTTGGAAATTTTATCTT+AGG	-	chr7.4:6101660-6101679	None:intergenic	20.0%
!!!	ATTTTATTATCGTATTCCTT+AGG	-	chr7.4:6101816-6101835	None:intergenic	20.0%
!	CGATAATTTACCATCAAAAT+TGG	+	chr7.4:6101890-6101909	MS.gene28790:CDS	25.0%
!	TATCAAAGAATAAACAAGTC+AGG	+	chr7.4:6101558-6101577	MS.gene28790:CDS	25.0%
	AAAGAATAAACAAGTCAGGT+CGG	+	chr7.4:6101562-6101581	MS.gene28790:CDS	30.0%
	CAATTTCTTCTCCTAAGAAA+TGG	+	chr7.4:6101676-6101695	MS.gene28790:CDS	30.0%
	TGTTCATATGTATAACTCGT+TGG	+	chr7.4:6101839-6101858	MS.gene28790:CDS	30.0%
	TTCTTAGGAGAAGAAATTGT+TGG	-	chr7.4:6101675-6101694	None:intergenic	30.0%
!	AAAGGAGTTTGAAAAAGTAC+TGG	+	chr7.4:6101520-6101539	MS.gene28790:CDS	30.0%
!	TTTGATGGTAAATTATCGAG+TGG	-	chr7.4:6101888-6101907	None:intergenic	30.0%
	TTCTTCTCCTAAGAAATGGA+TGG	+	chr7.4:6101680-6101699	MS.gene28790:CDS	35.0%
!	TATGTATAACTCGTTGGTTG+TGG	+	chr7.4:6101845-6101864	MS.gene28790:CDS	35.0%
!	TTATCGTATTCCTTAGGTTG+TGG	-	chr7.4:6101810-6101829	None:intergenic	35.0%
!!	AAAGTACTGGAAGAGAAGAA+AGG	+	chr7.4:6101533-6101552	MS.gene28790:CDS	35.0%
!!	AAGTACTGGAAGAGAAGAAA+GGG	+	chr7.4:6101534-6101553	MS.gene28790:CDS	35.0%
	ATCCACACCATCCATTTCTT+AGG	-	chr7.4:6101690-6101709	None:intergenic	40.0%
	CCACCAGAACAAAGAAGAAA+TGG	+	chr7.4:6101619-6101638	MS.gene28790:CDS	40.0%
	CCAGAACAAAGAAGAAATGG+AGG	+	chr7.4:6101622-6101641	MS.gene28790:CDS	40.0%
	CCATTTCTTCTTTGTTCTGG+TGG	-	chr7.4:6101622-6101641	None:intergenic	40.0%
	CCTCCATTTCTTCTTTGTTC+TGG	-	chr7.4:6101625-6101644	None:intergenic	40.0%
!	GTTTCTGAGCCCAATTTTGA+TGG	-	chr7.4:6101903-6101922	None:intergenic	40.0%
	AATGACCCCACATGTGGATT+TGG	+	chr7.4:6101777-6101796	MS.gene28790:CDS	45.0%
	ACCAAGCATCATCCTCACAT+AGG	-	chr7.4:6101721-6101740	None:intergenic	45.0%
	ATGACCCCACATGTGGATTT+GGG	+	chr7.4:6101778-6101797	MS.gene28790:CDS	45.0%
	CTCCTAAGAAATGGATGGTG+TGG	+	chr7.4:6101685-6101704	MS.gene28790:CDS	45.0%
	TCCTATGTGAGGATGATGCT+TGG	+	chr7.4:6101717-6101736	MS.gene28790:CDS	45.0%
	TGTGAGGATGATGCTTGGTT+TGG	+	chr7.4:6101722-6101741	MS.gene28790:CDS	45.0%
	AGCTTCAATGACCCCACATG+TGG	+	chr7.4:6101771-6101790	MS.gene28790:CDS	50.0%
	GGATGAGAGACTCCTATGTG+AGG	+	chr7.4:6101706-6101725	MS.gene28790:CDS	50.0%
!	AACTCGTTGGTTGTGGCTCA+GGG	+	chr7.4:6101852-6101871	MS.gene28790:CDS	50.0%
!	TAACTCGTTGGTTGTGGCTC+AGG	+	chr7.4:6101851-6101870	MS.gene28790:CDS	50.0%
!!	GCTTGGTTTGGCTAACTCGA+AGG	+	chr7.4:6101734-6101753	MS.gene28790:CDS	50.0%
	AAGACGAACACGGCGAAGCT+TGG	+	chr7.4:6101585-6101604	MS.gene28790:CDS	55.0%
	ACCCCACATGTGGATTTGGG+AGG	+	chr7.4:6101781-6101800	MS.gene28790:CDS	55.0%
	AGCCCTCCCAAATCCACATG+TGG	-	chr7.4:6101787-6101806	None:intergenic	55.0%
	CCCCACATGTGGATTTGGGA+GGG	+	chr7.4:6101782-6101801	MS.gene28790:CDS	55.0%
	CCCTCCCAAATCCACATGTG+GGG	-	chr7.4:6101785-6101804	None:intergenic	55.0%
	GCCCTCCCAAATCCACATGT+GGG	-	chr7.4:6101786-6101805	None:intergenic	55.0%
	GGTTGTGGCTCAGGGACATT+TGG	+	chr7.4:6101860-6101879	MS.gene28790:CDS	55.0%
	GTCAGGTCGGAAGACGAACA+CGG	+	chr7.4:6101575-6101594	MS.gene28790:CDS	55.0%
	AACACGGCGAAGCTTGGAGG+AGG	+	chr7.4:6101591-6101610	MS.gene28790:CDS	60.0%
	ACGAACACGGCGAAGCTTGG+AGG	+	chr7.4:6101588-6101607	MS.gene28790:CDS	60.0%
	TGGGAGGGCTCCACAACCTA+AGG	+	chr7.4:6101797-6101816	MS.gene28790:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	6101507	6101929	6101507	ID=MS.gene28790
chr7.4	mRNA	6101507	6101929	6101507	ID=MS.gene28790.t1;Parent=MS.gene28790
chr7.4	exon	6101507	6101929	6101507	ID=MS.gene28790.t1.exon1;Parent=MS.gene28790.t1
chr7.4	CDS	6101507	6101929	6101507	ID=cds.MS.gene28790.t1;Parent=MS.gene28790.t1

Gene Sequence

>MS.gene28790

ATGGAATGCATGCAAAGGAGTTTGAAAAAGTACTGGAAGAGAAGAAAGGGATATCAAAGAATAAACAAGTCAGGTCGGAAGACGAACACGGCGAAGCTTGGAGGAGGAGCAACCACCAGAACAAAGAAGAAATGGAGGATCAAAATTTCTCCTAAGATAAAATTTCCAACAATTTCTTCTCCTAAGAAATGGATGGTGTGGATGAGAGACTCCTATGTGAGGATGATGCTTGGTTTGGCTAACTCGAAGGTAATGAATTTGACTAGCTTCAATGACCCCACATGTGGATTTGGGAGGGCTCCACAACCTAAGGAATACGATAATAAAATGCTTGTTCATATGTATAACTCGTTGGTTGTGGCTCAGGGACATTTGGTTCCACTCGATAATTTACCATCAAAATTGGGCTCAGAAACAGCTTGA

Protein sequence

>MS.gene28790.t1

MECMQRSLKKYWKRRKGYQRINKSGRKTNTAKLGGGATTRTKKKWRIKISPKIKFPTISSPKKWMVWMRDSYVRMMLGLANSKVMNLTSFNDPTCGFGRAPQPKEYDNKMLVHMYNSLVVAQGHLVPLDNLPSKLGSETA