AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025894

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025894.t1 MTR_7g117670 93.664 584 21 5 1 578 1 574 0.0 1035
MS.gene025894.t1 MTR_8g027495 57.528 631 182 16 8 571 12 623 0.0 619
MS.gene025894.t1 MTR_7g096350 36.348 575 267 21 10 566 9 502 1.14e-97 306
MS.gene025894.t1 MTR_5g030430 41.704 223 105 5 372 572 458 677 4.69e-42 161
MS.gene025894.t1 MTR_5g030430 27.119 236 154 3 8 239 3 224 5.09e-21 97.8
MS.gene025894.t1 MTR_8g067280 42.365 203 85 5 388 565 444 639 2.55e-40 155
MS.gene025894.t1 MTR_8g067280 31.944 216 115 9 48 252 52 246 1.27e-17 87.0
MS.gene025894.t1 MTR_8g024790 38.372 172 97 1 386 548 271 442 2.03e-33 133
MS.gene025894.t1 MTR_8g024770 37.566 189 104 4 386 561 259 446 7.72e-31 125
MS.gene025894.t1 MTR_5g030420 30.049 203 124 4 42 240 20 208 1.84e-18 85.5
MS.gene025894.t1 MTR_5g014600 30.058 173 98 2 382 532 125 296 2.10e-18 87.0
MS.gene025894.t1 MTR_5g030420 30.049 203 124 4 42 240 20 208 2.55e-18 85.5
MS.gene025894.t1 MTR_4g097950 37.097 124 57 2 406 511 70 190 1.70e-17 82.8
MS.gene025894.t1 MTR_5g014640 37.302 126 68 4 393 513 145 264 2.47e-16 80.9
MS.gene025894.t1 MTR_4g067010 31.515 165 103 3 393 551 137 297 5.10e-16 79.7
MS.gene025894.t1 MTR_5g014520 28.108 185 122 4 389 564 142 324 5.95e-16 79.7
MS.gene025894.t1 MTR_2g010450 29.412 136 87 1 406 532 177 312 7.14e-16 79.7
MS.gene025894.t1 MTR_4g092700 57.627 59 25 0 404 462 156 214 7.70e-16 79.3
MS.gene025894.t1 MTR_2g104550 33.333 153 74 4 384 508 97 249 7.92e-16 79.0
MS.gene025894.t1 MTR_5g014560 27.957 186 112 4 393 563 119 297 1.12e-15 78.6
MS.gene025894.t1 MTR_0011s0210 32.298 161 97 4 399 551 180 336 1.36e-14 75.9
MS.gene025894.t1 MTR_0250s0040 32.143 168 98 6 383 541 71 231 3.03e-14 73.6
MS.gene025894.t1 MTR_0246s0050 29.651 172 107 4 387 553 136 298 3.37e-14 74.3
MS.gene025894.t1 MTR_1g072320 22.843 197 131 6 50 240 7 188 3.72e-14 75.9
MS.gene025894.t1 MTR_1g072320 46.512 86 44 2 390 473 453 538 9.12e-14 74.7
MS.gene025894.t1 MTR_4g098035 29.651 172 107 4 387 553 157 319 3.79e-14 74.3
MS.gene025894.t1 MTR_4g066380 49.333 75 34 1 387 461 138 208 4.34e-14 73.9
MS.gene025894.t1 MTR_6g047550 34.532 139 76 4 328 463 104 230 5.10e-14 73.2
MS.gene025894.t1 MTR_0246s0020 51.429 70 28 2 392 461 190 253 6.74e-14 73.9
MS.gene025894.t1 MTR_0246s0020 51.429 70 28 2 392 461 190 253 7.62e-14 73.9
MS.gene025894.t1 MTR_2g084280 31.333 150 97 3 410 553 137 286 8.29e-14 73.2
MS.gene025894.t1 MTR_0011s0260 31.677 161 98 3 399 551 180 336 8.36e-14 73.6
MS.gene025894.t1 MTR_8g098275 52.308 65 31 0 402 466 240 304 8.73e-14 74.3
MS.gene025894.t1 MTR_8g098275 52.308 65 31 0 402 466 304 368 1.15e-13 73.9
MS.gene025894.t1 MTR_3g498695 52.239 67 32 0 404 470 296 362 1.20e-13 73.9
MS.gene025894.t1 MTR_3g498695 52.239 67 32 0 404 470 296 362 1.20e-13 73.9
MS.gene025894.t1 MTR_4g097940 35.897 117 66 3 395 506 148 260 1.21e-13 72.8
MS.gene025894.t1 MTR_8g098275 52.308 65 31 0 402 466 397 461 1.70e-13 73.9
MS.gene025894.t1 MTR_4g057270 30.588 170 105 5 396 553 140 308 1.71e-13 72.4
MS.gene025894.t1 MTR_2g038040 58.182 55 23 0 407 461 53 107 1.82e-13 70.9
MS.gene025894.t1 MTR_1g112680 41.053 95 52 2 369 463 284 374 2.18e-13 73.2
MS.gene025894.t1 MTR_6g047570 26.423 246 158 7 313 547 75 308 2.72e-13 71.6
MS.gene025894.t1 MTR_4g066460 49.333 75 34 1 387 461 140 210 3.30e-13 71.2
MS.gene025894.t1 MTR_4g009110 26.702 191 106 5 333 508 116 287 4.69e-13 71.2
MS.gene025894.t1 MTR_7g083900 54.098 61 28 0 404 464 284 344 5.85e-13 71.6
MS.gene025894.t1 MTR_7g083900 54.098 61 28 0 404 464 284 344 6.08e-13 71.6
MS.gene025894.t1 MTR_2g104490 33.600 125 68 3 394 508 126 245 6.24e-13 70.1
MS.gene025894.t1 MTR_2g104490 33.071 127 70 3 392 508 124 245 7.02e-13 70.1
MS.gene025894.t1 MTR_4g009540 23.762 202 139 3 380 566 126 327 2.27e-12 68.9
MS.gene025894.t1 MTR_2g022280 26.404 178 108 3 405 560 1 177 2.29e-12 66.6
MS.gene025894.t1 MTR_4g009540 26.573 143 91 2 380 508 126 268 5.30e-12 67.4
MS.gene025894.t1 MTR_2g104560 25.000 168 119 3 407 567 55 222 6.10e-12 66.2
MS.gene025894.t1 MTR_4g097920 46.154 65 31 1 395 459 133 193 2.10e-11 65.9
MS.gene025894.t1 MTR_3g030700 43.662 71 40 0 394 464 184 254 2.37e-11 66.2
MS.gene025894.t1 MTR_4g097920 46.154 65 31 1 395 459 148 208 2.45e-11 65.9
MS.gene025894.t1 MTR_3g104100 48.387 62 29 1 410 471 199 257 5.79e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025894.t1 AT1G01260 48.052 616 248 15 4 577 3 588 3.34e-175 509
MS.gene025894.t1 AT1G01260 48.052 616 248 15 4 577 3 588 3.34e-175 509
MS.gene025894.t1 AT1G01260 48.052 616 248 15 4 577 3 588 3.34e-175 509
MS.gene025894.t1 AT2G46510 47.368 589 241 16 9 572 8 552 1.67e-163 478
MS.gene025894.t1 AT4G17880 32.914 556 301 17 48 565 61 582 1.75e-72 243
MS.gene025894.t1 AT4G16430 49.730 185 74 3 385 566 299 467 1.65e-53 189
MS.gene025894.t1 AT4G16430 37.500 240 120 6 10 240 8 226 9.36e-37 143
MS.gene025894.t1 AT5G46760 44.444 216 106 5 361 564 371 584 2.32e-45 169
MS.gene025894.t1 AT1G32640 44.211 190 90 3 387 564 433 618 1.01e-38 151
MS.gene025894.t1 AT1G32640 32.828 198 117 4 48 240 66 252 7.11e-20 94.0
MS.gene025894.t1 AT5G46830 30.080 502 272 18 67 548 49 491 6.04e-37 144
MS.gene025894.t1 AT5G57150 33.645 214 111 8 386 578 40 243 3.81e-20 90.5
MS.gene025894.t1 AT5G57150 33.889 180 96 6 386 548 40 213 8.39e-19 85.9
MS.gene025894.t1 AT5G57150 33.889 180 96 6 386 548 41 214 1.48e-18 85.1
MS.gene025894.t1 AT5G57150 33.889 180 96 6 386 548 41 214 1.53e-18 86.3
MS.gene025894.t1 AT5G57150 33.889 180 96 6 386 548 41 214 1.68e-18 85.1
MS.gene025894.t1 AT4G00870 37.500 136 74 1 405 529 248 383 2.73e-18 87.8
MS.gene025894.t1 AT4G09820 28.194 227 145 5 36 253 2 219 2.91e-18 88.6
MS.gene025894.t1 AT4G09820 34.351 131 55 3 382 508 350 453 6.08e-16 81.3
MS.gene025894.t1 AT4G37850 28.194 227 123 5 358 556 98 312 3.18e-18 86.7
MS.gene025894.t1 AT4G37850 28.194 227 123 5 358 556 98 312 3.18e-18 86.7
MS.gene025894.t1 AT5G41315 29.167 216 127 4 41 240 6 211 1.92e-16 83.2
MS.gene025894.t1 AT5G41315 48.571 70 36 0 400 469 440 509 9.81e-14 74.7
MS.gene025894.t1 AT5G41315 29.167 216 127 4 41 240 1 206 1.93e-16 83.2
MS.gene025894.t1 AT5G41315 48.571 70 36 0 400 469 435 504 9.58e-14 74.7
MS.gene025894.t1 AT5G41315 29.167 216 127 4 41 240 9 214 2.13e-16 83.2
MS.gene025894.t1 AT5G41315 48.571 70 36 0 400 469 443 512 9.85e-14 74.7
MS.gene025894.t1 AT1G63650 29.148 223 128 8 41 247 1 209 7.02e-16 81.3
MS.gene025894.t1 AT1G63650 48.529 68 35 0 402 469 401 468 1.65e-13 73.9
MS.gene025894.t1 AT1G63650 29.148 223 128 8 41 247 1 209 7.02e-16 81.3
MS.gene025894.t1 AT1G63650 48.529 68 35 0 402 469 401 468 1.65e-13 73.9
MS.gene025894.t1 AT1G63650 29.148 223 128 8 41 247 1 209 7.02e-16 81.3
MS.gene025894.t1 AT1G63650 48.529 68 35 0 402 469 401 468 1.65e-13 73.9
MS.gene025894.t1 AT4G29930 31.757 148 94 3 390 536 43 184 1.68e-14 73.9
MS.gene025894.t1 AT4G29930 29.091 165 110 3 390 553 43 201 1.75e-14 73.6
MS.gene025894.t1 AT2G22750 32.911 158 89 3 388 532 140 293 3.46e-14 74.3
MS.gene025894.t1 AT2G22750 32.911 158 89 3 388 532 140 293 3.59e-14 74.3
MS.gene025894.t1 AT2G22750 32.911 158 89 3 388 532 112 265 3.64e-14 73.9
MS.gene025894.t1 AT1G12860 53.731 67 31 0 404 470 265 331 3.74e-14 75.1
MS.gene025894.t1 AT2G22750 32.911 158 89 3 388 532 136 289 3.78e-14 74.3
MS.gene025894.t1 AT2G22750 32.911 158 89 3 388 532 112 265 3.84e-14 73.9
MS.gene025894.t1 AT1G12860 53.731 67 31 0 404 470 265 331 4.21e-14 75.1
MS.gene025894.t1 AT4G00480 30.151 199 104 8 67 243 36 221 6.75e-14 74.7
MS.gene025894.t1 AT4G29930 28.144 167 112 4 390 553 43 204 7.21e-14 71.6
MS.gene025894.t1 AT4G00480 30.151 199 104 8 67 243 36 221 8.82e-14 74.7
MS.gene025894.t1 AT2G16910 33.728 169 73 5 410 546 318 479 1.02e-13 74.3
MS.gene025894.t1 AT4G00480 30.151 199 104 8 67 243 36 221 1.30e-13 74.3
MS.gene025894.t1 AT3G26744 55.738 61 27 0 404 464 305 365 2.46e-13 72.8
MS.gene025894.t1 AT4G29930 48.611 72 32 1 390 461 43 109 2.54e-13 69.3
MS.gene025894.t1 AT3G26744 55.738 61 27 0 404 464 305 365 2.68e-13 72.8
MS.gene025894.t1 AT3G26744 55.738 61 27 0 404 464 305 365 2.68e-13 72.8
MS.gene025894.t1 AT3G26744 55.738 61 27 0 404 464 305 365 2.68e-13 72.8
MS.gene025894.t1 AT4G29930 48.611 72 32 1 390 461 43 109 3.09e-13 70.5
MS.gene025894.t1 AT4G29930 48.611 72 32 1 390 461 43 109 3.09e-13 70.5
MS.gene025894.t1 AT4G29930 48.611 72 32 1 390 461 43 109 3.85e-13 70.1
MS.gene025894.t1 AT2G22760 33.628 113 64 2 407 508 120 232 4.82e-13 70.5
MS.gene025894.t1 AT2G22760 33.628 113 64 2 407 508 120 232 5.62e-13 70.5
MS.gene025894.t1 AT2G22770 50.000 66 30 1 400 462 123 188 5.43e-12 67.8
MS.gene025894.t1 AT2G28160 45.312 64 35 0 410 473 135 198 5.97e-12 67.4
MS.gene025894.t1 AT2G28160 45.312 64 35 0 410 473 135 198 6.41e-12 67.4
MS.gene025894.t1 AT5G65640 29.747 158 100 3 403 554 175 327 5.70e-11 64.7

Find 109 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATTCAACACAACTTT+TGG 0.177209 7.1:-734978 None:intergenic
ATTGGAATTACGCAATTTAT+TGG 0.206579 7.1:+734367 MS.gene025894:CDS
CTGACTTAATTACAAAATTA+TGG 0.220746 7.1:-735796 None:intergenic
TTATTGCTTTCGTTCTTTCT+TGG 0.223221 7.1:+734746 MS.gene025894:CDS
TGCGTAATTCCAATTGAAAT+TGG 0.241887 7.1:-734358 None:intergenic
ATGCCTATATGGACAAAGTT+TGG 0.244044 7.1:-735889 None:intergenic
TTCAACAAATCAAAGATTTC+AGG 0.251699 7.1:-734849 None:intergenic
CCGGGTCCACCATACCCTTT+TGG 0.264704 7.1:-734675 None:intergenic
CTCTATTTATCATGCCTATA+TGG 0.268867 7.1:-735900 None:intergenic
CAGAAGTTGCATAAAGCTTT+TGG 0.288502 7.1:+734564 MS.gene025894:CDS
GAAACTTGCCATTAGGAAAA+TGG 0.290252 7.1:+735025 MS.gene025894:CDS
GATGAAGACAATTACGCTTT+TGG 0.306225 7.1:+734594 MS.gene025894:CDS
TCTGCCTCCACATGATTTAG+AGG 0.320999 7.1:-735380 None:intergenic
GATCAATAAAGCGCATGATA+TGG 0.340119 7.1:+734175 MS.gene025894:CDS
ACCATGGTTGGAGCTATTCT+TGG 0.347843 7.1:+734213 MS.gene025894:CDS
TGCCTATATGGACAAAGTTT+GGG 0.350641 7.1:-735888 None:intergenic
ATTCTTCATCCAATTTCAAT+TGG 0.357065 7.1:+734349 MS.gene025894:CDS
ACCAGAAGCAAAACACTTAC+CGG 0.359802 7.1:-734694 None:intergenic
TTGTCCATCTTGGAAATATT+CGG 0.360963 7.1:-735458 None:intergenic
TTAGGGAGAAACTTGCCATT+AGG 0.364646 7.1:+735018 MS.gene025894:CDS
GGACAAAGTTTGGGTTTGTA+AGG 0.367197 7.1:-735879 None:intergenic
AATAACCGGTCTCGCAGAAT+TGG 0.375253 7.1:-735258 None:intergenic
CGACTTTCTAAGTCAAAATC+TGG 0.375807 7.1:+734390 MS.gene025894:CDS
ACTCCACCTTCTTCTTCGTT+TGG 0.377124 7.1:-734453 None:intergenic
CTGGTGATTACGTTCTTTGT+TGG 0.387307 7.1:+734409 MS.gene025894:CDS
TGCATTGGCATCTGCCTTTG+TGG 0.387976 7.1:-735206 None:intergenic
CTAGGTCTCCTTTCATCGCT+AGG 0.390719 7.1:-735320 None:intergenic
TATTCTTGGTGCTCGAGCTT+TGG 0.402534 7.1:+734227 MS.gene025894:CDS
CATTTGGAAGCAGTACTTCC+AGG 0.403231 7.1:+735561 MS.gene025894:CDS
GTTTCGATTAGTTTCGAAAC+AGG 0.406082 7.1:-735710 None:intergenic
ATTCGGTACAACAGCTCTTA+AGG 0.408610 7.1:-735441 None:intergenic
ATGGTATTTGTGGTTCTACT+TGG 0.409907 7.1:+735096 MS.gene025894:CDS
AACTTTCCTAGTGCTAGAAA+TGG 0.411288 7.1:+735077 MS.gene025894:CDS
TCTTTCTTGGCTAAATCTGC+TGG 0.412934 7.1:+734759 MS.gene025894:CDS
CGCGGAAGGAAGCCTGCTAA+TGG 0.414504 7.1:+735347 MS.gene025894:CDS
TGGTGATTACGTTCTTTGTT+GGG 0.415145 7.1:+734410 MS.gene025894:CDS
TAAAGATGCCTTGTCCATCT+TGG 0.422075 7.1:-735468 None:intergenic
TGAAACAAAGTATGAAGAAG+TGG 0.425466 7.1:+734535 MS.gene025894:CDS
TGCTTCTAATGGTGTGAGAA+AGG 0.436502 7.1:+735148 MS.gene025894:CDS
AGTTTGGGTTTGTAAGGAGT+TGG 0.441750 7.1:-735873 None:intergenic
TTGCTTCTGGTAAACATTTG+TGG 0.442625 7.1:+734706 MS.gene025894:CDS
GAATACTAGAGTTTCTCTTC+CGG 0.446637 7.1:+735622 MS.gene025894:CDS
TTGTAAGGAGTTGGATTCTC+GGG 0.450320 7.1:-735864 None:intergenic
GAGTCAGAGAGAGAATCATT+TGG 0.451334 7.1:+735545 MS.gene025894:CDS
ATGGACAAGGCATCTTTACT+TGG 0.451417 7.1:+735473 MS.gene025894:CDS
TAGAGTTTCTCTTCCGGATG+TGG 0.452720 7.1:+735628 MS.gene025894:CDS
GGTTCGTCCCTTCCATTAGC+AGG 0.455729 7.1:-735359 None:intergenic
GGCTTCCTTCCGCGCTTCCT+AGG 0.458293 7.1:-735338 None:intergenic
TGGTATTTGTGGTTCTACTT+GGG 0.461524 7.1:+735097 MS.gene025894:CDS
TACGTTCTTTGTTGGGCAGA+TGG 0.462040 7.1:+734417 MS.gene025894:CDS
AGTGCTAGAAATGGTATTTG+TGG 0.472584 7.1:+735086 MS.gene025894:CDS
TTTGTAAGGAGTTGGATTCT+CGG 0.473242 7.1:-735865 None:intergenic
CTTTCTTCATTGACCACACT+AGG 0.491428 7.1:-734930 None:intergenic
GAAGAAAGTACAGTTTCAAA+TGG 0.494078 7.1:+734945 MS.gene025894:CDS
TGCTGCAATCAACTTGTCCT+TGG 0.495738 7.1:-735837 None:intergenic
AATGATAATGATTGTGTGGC+AGG 0.499914 7.1:-734903 None:intergenic
TCAAGCGAAACTCAAGATCA+TGG 0.503060 7.1:+735523 MS.gene025894:CDS
ACGTTCTTTGTTGGGCAGAT+GGG 0.511903 7.1:+734418 MS.gene025894:CDS
AGTACTTCCAGGGATGGATC+AGG 0.512089 7.1:+735572 MS.gene025894:CDS
GAGGGCCAATTCTGCGAGAC+CGG 0.516559 7.1:+735253 MS.gene025894:CDS
TCAAGATGAGGTAATAGTAA+AGG 0.517416 7.1:+735664 MS.gene025894:CDS
AACCCAAACTTTGTCCATAT+AGG 0.517452 7.1:+735886 MS.gene025894:CDS
GGTATTTGTGGTTCTACTTG+GGG 0.519442 7.1:+735098 MS.gene025894:CDS
CGAGTTGCCTGATCCATCCC+TGG 0.522358 7.1:-735579 None:intergenic
CCGGAAAAGTCAATTTGCAT+TGG 0.525652 7.1:-735221 None:intergenic
GAGACCGGTTATTGCTGAAT+CGG 0.527481 7.1:+735268 MS.gene025894:CDS
TTGGCATCTGCCTTTGTGGT+TGG 0.531676 7.1:-735202 None:intergenic
ATTGGCCCTCGAATTTGCTC+CGG 0.532064 7.1:-735240 None:intergenic
AGTTCCGATTCAGCAATAAC+CGG 0.536707 7.1:-735272 None:intergenic
CCAAAAGGGTATGGTGGACC+CGG 0.545307 7.1:+734675 MS.gene025894:CDS
GGAAGCAGTACTTCCAGGGA+TGG 0.552030 7.1:+735566 MS.gene025894:CDS
TGTACCGAATATTTCCAAGA+TGG 0.552909 7.1:+735454 MS.gene025894:CDS
GAAGCTTGAATATCCACATC+CGG 0.553300 7.1:-735641 None:intergenic
ATGTGGAGGCAGAGAGGCAA+AGG 0.553319 7.1:+735390 MS.gene025894:CDS
CCAGAAGCAAAACACTTACC+GGG 0.554206 7.1:-734693 None:intergenic
GAAGAAGAGAAAACCATGGT+TGG 0.555055 7.1:+734201 MS.gene025894:CDS
GAAACAAAGTATGAAGAAGT+GGG 0.558139 7.1:+734536 MS.gene025894:CDS
TCTACTTGGGGAGTGAATCA+AGG 0.559151 7.1:+735110 MS.gene025894:CDS
GAAGGAAGCCTGCTAATGGA+AGG 0.564326 7.1:+735351 MS.gene025894:CDS
ATTTGGAAGCAGTACTTCCA+GGG 0.565753 7.1:+735562 MS.gene025894:CDS
CTGATCAACCTAGCGATGAA+AGG 0.566880 7.1:+735312 MS.gene025894:CDS
AACTGTTTATCAAATGAGAG+TGG 0.571748 7.1:+734264 MS.gene025894:CDS
TCTGAGTCTTCGCATAGAAG+AGG 0.573258 7.1:+734485 MS.gene025894:CDS
CAAATACCATTTCTAGCACT+AGG 0.582823 7.1:-735083 None:intergenic
GGAGACCTAGGAAGCGCGGA+AGG 0.585153 7.1:+735333 MS.gene025894:CDS
GAGAGTGGATTGATTGCTGT+TGG 0.590794 7.1:+734279 MS.gene025894:CDS
CTGTGTCGGTTACACGGTCA+AGG 0.590950 7.1:-734617 None:intergenic
ACTAATCGAAACATTCAAAG+AGG 0.592504 7.1:+735721 MS.gene025894:CDS
ACATCTCTGTGTCGGTTACA+CGG 0.595335 7.1:-734623 None:intergenic
AGAGAACCAAACGAAGAAGA+AGG 0.608578 7.1:+734447 MS.gene025894:CDS
ACCAAGAATAGCTCCAACCA+TGG 0.609004 7.1:-734214 None:intergenic
ATTATCATTGTATCCTAGTG+TGG 0.610408 7.1:+734917 MS.gene025894:CDS
GGAACTTGTTGCTGATGTTG+AGG 0.613094 7.1:+735289 MS.gene025894:CDS
GAACCAAACGAAGAAGAAGG+TGG 0.613103 7.1:+734450 MS.gene025894:CDS
CTAGCGATGAAAGGAGACCT+AGG 0.617300 7.1:+735321 MS.gene025894:CDS
TATTCAAGCTTCTCAAGATG+AGG 0.619645 7.1:+735652 MS.gene025894:CDS
TCAACAACACCAAAATCAGT+AGG 0.632462 7.1:-734804 None:intergenic
TGGGGAGTGAATCAAGGTCA+AGG 0.636279 7.1:+735116 MS.gene025894:CDS
GTTGTGTTGAATTTGAACAA+TGG 0.637607 7.1:+734984 MS.gene025894:CDS
GGACGAACCTCTAAATCATG+TGG 0.640327 7.1:+735373 MS.gene025894:CDS
AAGGAAGCCTGCTAATGGAA+GGG 0.640978 7.1:+735352 MS.gene025894:CDS
GAAAGGAGACCTAGGAAGCG+CGG 0.657593 7.1:+735329 MS.gene025894:CDS
GAATATTTCCAAGATGGACA+AGG 0.676800 7.1:+735460 MS.gene025894:CDS
ATACAATGATAATGATTGTG+TGG 0.679020 7.1:-734907 None:intergenic
TAAATCATGTGGAGGCAGAG+AGG 0.679660 7.1:+735384 MS.gene025894:CDS
CGAACCTCTAAATCATGTGG+AGG 0.682050 7.1:+735376 MS.gene025894:CDS
AGGATCTGAACAGCTTACCA+AGG 0.700634 7.1:+735820 MS.gene025894:CDS
ATAGCAACTACCAACCACAA+AGG 0.712491 7.1:+735192 MS.gene025894:CDS
GAATGAAGAAGAGAAAACCA+TGG 0.720626 7.1:+734197 MS.gene025894:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTGACTTAATTACAAAATTA+TGG - chr7.1:735799-735818 None:intergenic 20.0%
!!! AATTTTGTAATTAAGTCAGA+AGG + chr7.1:735800-735819 MS.gene025894:CDS 20.0%
! ATTCTTCATCCAATTTCAAT+TGG + chr7.1:734349-734368 MS.gene025894:CDS 25.0%
! ATTGGAATTACGCAATTTAT+TGG + chr7.1:734367-734386 MS.gene025894:CDS 25.0%
! TTCAACAAATCAAAGATTTC+AGG - chr7.1:734852-734871 None:intergenic 25.0%
!! ATACAATGATAATGATTGTG+TGG - chr7.1:734910-734929 None:intergenic 25.0%
!! ATTACTATCCATTTTCCTAA+TGG - chr7.1:735036-735055 None:intergenic 25.0%
!! TTCAAATTCAACACAACTTT+TGG - chr7.1:734981-735000 None:intergenic 25.0%
AAAGAAGAAGAAGAAGAAGA+AGG + chr7.1:734510-734529 MS.gene025894:CDS 30.0%
AACTGTTTATCAAATGAGAG+TGG + chr7.1:734264-734283 MS.gene025894:CDS 30.0%
ACTAATCGAAACATTCAAAG+AGG + chr7.1:735721-735740 MS.gene025894:CDS 30.0%
ATTATCATTGTATCCTAGTG+TGG + chr7.1:734917-734936 MS.gene025894:CDS 30.0%
GAAACAAAGTATGAAGAAGT+GGG + chr7.1:734536-734555 MS.gene025894:CDS 30.0%
GTTGTGTTGAATTTGAACAA+TGG + chr7.1:734984-735003 MS.gene025894:CDS 30.0%
TAAAAAGAACATCTCTGTGT+CGG - chr7.1:734634-734653 None:intergenic 30.0%
TCAAGATGAGGTAATAGTAA+AGG + chr7.1:735664-735683 MS.gene025894:CDS 30.0%
TGAAACAAAGTATGAAGAAG+TGG + chr7.1:734535-734554 MS.gene025894:CDS 30.0%
TGCGTAATTCCAATTGAAAT+TGG - chr7.1:734361-734380 None:intergenic 30.0%
TTATTGCTTTCGTTCTTTCT+TGG + chr7.1:734746-734765 MS.gene025894:CDS 30.0%
TTGTCCATCTTGGAAATATT+CGG - chr7.1:735461-735480 None:intergenic 30.0%
! ATGTACTTCTTTTTCCCAAA+AGG + chr7.1:734660-734679 MS.gene025894:CDS 30.0%
! GAAGAAAGTACAGTTTCAAA+TGG + chr7.1:734945-734964 MS.gene025894:CDS 30.0%
! TGTACTTCTTTTTCCCAAAA+GGG + chr7.1:734661-734680 MS.gene025894:CDS 30.0%
!!! GGTGAATTTTTTGCTTCTAA+TGG + chr7.1:735137-735156 MS.gene025894:CDS 30.0%
!!! GTTTTAGTACCTACTGATTT+TGG + chr7.1:734795-734814 MS.gene025894:CDS 30.0%
AACTTTCCTAGTGCTAGAAA+TGG + chr7.1:735077-735096 MS.gene025894:CDS 35.0%
AGTGCTAGAAATGGTATTTG+TGG + chr7.1:735086-735105 MS.gene025894:CDS 35.0%
CGACTTTCTAAGTCAAAATC+TGG + chr7.1:734390-734409 MS.gene025894:CDS 35.0%
GAAACTTGCCATTAGGAAAA+TGG + chr7.1:735025-735044 MS.gene025894:CDS 35.0%
GAATACTAGAGTTTCTCTTC+CGG + chr7.1:735622-735641 MS.gene025894:CDS 35.0%
GAATATTTCCAAGATGGACA+AGG + chr7.1:735460-735479 MS.gene025894:CDS 35.0%
GAATGAAGAAGAGAAAACCA+TGG + chr7.1:734197-734216 MS.gene025894:CDS 35.0%
GATCAATAAAGCGCATGATA+TGG + chr7.1:734175-734194 MS.gene025894:CDS 35.0%
TATTCAAGCTTCTCAAGATG+AGG + chr7.1:735652-735671 MS.gene025894:CDS 35.0%
TCAACAACACCAAAATCAGT+AGG - chr7.1:734807-734826 None:intergenic 35.0%
TGGTGATTACGTTCTTTGTT+GGG + chr7.1:734410-734429 MS.gene025894:CDS 35.0%
TGTACCGAATATTTCCAAGA+TGG + chr7.1:735454-735473 MS.gene025894:CDS 35.0%
TTGCTTCTGGTAAACATTTG+TGG + chr7.1:734706-734725 MS.gene025894:CDS 35.0%
TTTGTAAGGAGTTGGATTCT+CGG - chr7.1:735868-735887 None:intergenic 35.0%
! AAGTTGCATAAAGCTTTTGG+TGG + chr7.1:734567-734586 MS.gene025894:CDS 35.0%
! AATGATAATGATTGTGTGGC+AGG - chr7.1:734906-734925 None:intergenic 35.0%
! ACAATGGTCGTACACATTTT+AGG + chr7.1:735000-735019 MS.gene025894:CDS 35.0%
! ATGGTATTTGTGGTTCTACT+TGG + chr7.1:735096-735115 MS.gene025894:CDS 35.0%
! ATGGTGTGAGAAAGGATTTT+AGG + chr7.1:735156-735175 MS.gene025894:CDS 35.0%
! CAATGGTCGTACACATTTTA+GGG + chr7.1:735001-735020 MS.gene025894:CDS 35.0%
! CAGAAGTTGCATAAAGCTTT+TGG + chr7.1:734564-734583 MS.gene025894:CDS 35.0%
! GATGAAGACAATTACGCTTT+TGG + chr7.1:734594-734613 MS.gene025894:CDS 35.0%
! TGGTATTTGTGGTTCTACTT+GGG + chr7.1:735097-735116 MS.gene025894:CDS 35.0%
! TTCTTTTTCCCAAAAGGGTA+TGG + chr7.1:734666-734685 MS.gene025894:CDS 35.0%
!! CAAATACCATTTCTAGCACT+AGG - chr7.1:735086-735105 None:intergenic 35.0%
!! CCAATGCAAATTGACTTTTC+CGG + chr7.1:735221-735240 MS.gene025894:CDS 35.0%
!! GTTTCGATTAGTTTCGAAAC+AGG - chr7.1:735713-735732 None:intergenic 35.0%
!!! GATTTTGGTGTTGTTGAGTT+GGG + chr7.1:734810-734829 MS.gene025894:CDS 35.0%
!!! TGATTTTGGTGTTGTTGAGT+TGG + chr7.1:734809-734828 MS.gene025894:CDS 35.0%
ACCAGAAGCAAAACACTTAC+CGG - chr7.1:734697-734716 None:intergenic 40.0%
AGAGAACCAAACGAAGAAGA+AGG + chr7.1:734447-734466 MS.gene025894:CDS 40.0%
AGTTCCGATTCAGCAATAAC+CGG - chr7.1:735275-735294 None:intergenic 40.0%
ATAGCAACTACCAACCACAA+AGG + chr7.1:735192-735211 MS.gene025894:CDS 40.0%
ATTCGGTACAACAGCTCTTA+AGG - chr7.1:735444-735463 None:intergenic 40.0%
ATTTGGAAGCAGTACTTCCA+GGG + chr7.1:735562-735581 MS.gene025894:CDS 40.0%
CCGGAAAAGTCAATTTGCAT+TGG - chr7.1:735224-735243 None:intergenic 40.0%
CTGGTGATTACGTTCTTTGT+TGG + chr7.1:734409-734428 MS.gene025894:CDS 40.0%
CTTTCTTCATTGACCACACT+AGG - chr7.1:734933-734952 None:intergenic 40.0%
GAAGAAGAGAAAACCATGGT+TGG + chr7.1:734201-734220 MS.gene025894:CDS 40.0%
GAAGCTTGAATATCCACATC+CGG - chr7.1:735644-735663 None:intergenic 40.0%
GAGTCAGAGAGAGAATCATT+TGG + chr7.1:735545-735564 MS.gene025894:CDS 40.0%
TAAAGATGCCTTGTCCATCT+TGG - chr7.1:735471-735490 None:intergenic 40.0%
TCAAGCGAAACTCAAGATCA+TGG + chr7.1:735523-735542 MS.gene025894:CDS 40.0%
TCTTTCTTGGCTAAATCTGC+TGG + chr7.1:734759-734778 MS.gene025894:CDS 40.0%
TGCTTCTAATGGTGTGAGAA+AGG + chr7.1:735148-735167 MS.gene025894:CDS 40.0%
TTAGGGAGAAACTTGCCATT+AGG + chr7.1:735018-735037 MS.gene025894:CDS 40.0%
TTGTAAGGAGTTGGATTCTC+GGG - chr7.1:735867-735886 None:intergenic 40.0%
! AGTTTGGGTTTGTAAGGAGT+TGG - chr7.1:735876-735895 None:intergenic 40.0%
! ATGGACAAGGCATCTTTACT+TGG + chr7.1:735473-735492 MS.gene025894:CDS 40.0%
! GGACAAAGTTTGGGTTTGTA+AGG - chr7.1:735882-735901 None:intergenic 40.0%
! GGTATTTGTGGTTCTACTTG+GGG + chr7.1:735098-735117 MS.gene025894:CDS 40.0%
!! TTTTTCCCAAAAGGGTATGG+TGG + chr7.1:734669-734688 MS.gene025894:CDS 40.0%
AAGGAAGCCTGCTAATGGAA+GGG + chr7.1:735352-735371 MS.gene025894:CDS 45.0%
AATAACCGGTCTCGCAGAAT+TGG - chr7.1:735261-735280 None:intergenic 45.0%
ACCAAGAATAGCTCCAACCA+TGG - chr7.1:734217-734236 None:intergenic 45.0%
ACCATGGTTGGAGCTATTCT+TGG + chr7.1:734213-734232 MS.gene025894:CDS 45.0%
ACGTTCTTTGTTGGGCAGAT+GGG + chr7.1:734418-734437 MS.gene025894:CDS 45.0%
ACTCCACCTTCTTCTTCGTT+TGG - chr7.1:734456-734475 None:intergenic 45.0%
AGGATCTGAACAGCTTACCA+AGG + chr7.1:735820-735839 MS.gene025894:CDS 45.0%
CATTTGGAAGCAGTACTTCC+AGG + chr7.1:735561-735580 MS.gene025894:CDS 45.0%
CCAGAAGCAAAACACTTACC+GGG - chr7.1:734696-734715 None:intergenic 45.0%
CGAACCTCTAAATCATGTGG+AGG + chr7.1:735376-735395 MS.gene025894:CDS 45.0%
CTGATCAACCTAGCGATGAA+AGG + chr7.1:735312-735331 MS.gene025894:CDS 45.0%
GAACCAAACGAAGAAGAAGG+TGG + chr7.1:734450-734469 MS.gene025894:CDS 45.0%
GAGACCGGTTATTGCTGAAT+CGG + chr7.1:735268-735287 MS.gene025894:CDS 45.0%
GGACGAACCTCTAAATCATG+TGG + chr7.1:735373-735392 MS.gene025894:CDS 45.0%
TAAATCATGTGGAGGCAGAG+AGG + chr7.1:735384-735403 MS.gene025894:CDS 45.0%
TACGTTCTTTGTTGGGCAGA+TGG + chr7.1:734417-734436 MS.gene025894:CDS 45.0%
TAGAGTTTCTCTTCCGGATG+TGG + chr7.1:735628-735647 MS.gene025894:CDS 45.0%
TCTACTTGGGGAGTGAATCA+AGG + chr7.1:735110-735129 MS.gene025894:CDS 45.0%
TCTGAGTCTTCGCATAGAAG+AGG + chr7.1:734485-734504 MS.gene025894:CDS 45.0%
TCTGCCTCCACATGATTTAG+AGG - chr7.1:735383-735402 None:intergenic 45.0%
TGCTGCAATCAACTTGTCCT+TGG - chr7.1:735840-735859 None:intergenic 45.0%
! ACTTTTCCGGAGCAAATTCG+AGG + chr7.1:735234-735253 MS.gene025894:CDS 45.0%
! CTTTTCCGGAGCAAATTCGA+GGG + chr7.1:735235-735254 MS.gene025894:CDS 45.0%
!! ACATCTCTGTGTCGGTTACA+CGG - chr7.1:734626-734645 None:intergenic 45.0%
!! GAGAGTGGATTGATTGCTGT+TGG + chr7.1:734279-734298 MS.gene025894:CDS 45.0%
!! GGAACTTGTTGCTGATGTTG+AGG + chr7.1:735289-735308 MS.gene025894:CDS 45.0%
!! TATTCTTGGTGCTCGAGCTT+TGG + chr7.1:734227-734246 MS.gene025894:CDS 45.0%
AGTACTTCCAGGGATGGATC+AGG + chr7.1:735572-735591 MS.gene025894:CDS 50.0%
CTAGCGATGAAAGGAGACCT+AGG + chr7.1:735321-735340 MS.gene025894:CDS 50.0%
CTAGGTCTCCTTTCATCGCT+AGG - chr7.1:735323-735342 None:intergenic 50.0%
GAAGGAAGCCTGCTAATGGA+AGG + chr7.1:735351-735370 MS.gene025894:CDS 50.0%
TGGGGAGTGAATCAAGGTCA+AGG + chr7.1:735116-735135 MS.gene025894:CDS 50.0%
TTGGCATCTGCCTTTGTGGT+TGG - chr7.1:735205-735224 None:intergenic 50.0%
! ATTGGCCCTCGAATTTGCTC+CGG - chr7.1:735243-735262 None:intergenic 50.0%
! CCCGGTAAGTGTTTTGCTTC+TGG + chr7.1:734693-734712 MS.gene025894:CDS 50.0%
! TGCATTGGCATCTGCCTTTG+TGG - chr7.1:735209-735228 None:intergenic 50.0%
ATGTGGAGGCAGAGAGGCAA+AGG + chr7.1:735390-735409 MS.gene025894:CDS 55.0%
GAAAGGAGACCTAGGAAGCG+CGG + chr7.1:735329-735348 MS.gene025894:CDS 55.0%
GGAAGCAGTACTTCCAGGGA+TGG + chr7.1:735566-735585 MS.gene025894:CDS 55.0%
GGTTCGTCCCTTCCATTAGC+AGG - chr7.1:735362-735381 None:intergenic 55.0%
! CGGGTCCACCATACCCTTTT+GGG - chr7.1:734677-734696 None:intergenic 55.0%
!! CCAAAAGGGTATGGTGGACC+CGG + chr7.1:734675-734694 MS.gene025894:CDS 55.0%
!! CTGTGTCGGTTACACGGTCA+AGG - chr7.1:734620-734639 None:intergenic 55.0%
CGAGTTGCCTGATCCATCCC+TGG - chr7.1:735582-735601 None:intergenic 60.0%
CGCGGAAGGAAGCCTGCTAA+TGG + chr7.1:735347-735366 MS.gene025894:CDS 60.0%
GAGGGCCAATTCTGCGAGAC+CGG + chr7.1:735253-735272 MS.gene025894:CDS 60.0%
! CCGGGTCCACCATACCCTTT+TGG - chr7.1:734678-734697 None:intergenic 60.0%
GGCTTCCTTCCGCGCTTCCT+AGG - chr7.1:735341-735360 None:intergenic 65.0%
! GGAGACCTAGGAAGCGCGGA+AGG + chr7.1:735333-735352 MS.gene025894:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 734171 735907 734171 ID=MS.gene025894
chr7.1 mRNA 734171 735907 734171 ID=MS.gene025894.t1;Parent=MS.gene025894
chr7.1 exon 734171 735907 734171 ID=MS.gene025894.t1.exon1;Parent=MS.gene025894.t1
chr7.1 CDS 734171 735907 734171 ID=cds.MS.gene025894.t1;Parent=MS.gene025894.t1
Gene Sequence

>MS.gene025894

ATGGGATCAATAAAGCGCATGATATGGAATGAAGAAGAGAAAACCATGGTTGGAGCTATTCTTGGTGCTCGAGCTTTGGATTTTTTGATAAATAACTGTTTATCAAATGAGAGTGGATTGATTGCTGTTGGTGAAAGTGTTGATTCTCTTCAAAACAAACTCTCTGATCTTGTTGAAAATTCTTCATCCAATTTCAATTGGAATTACGCAATTTATTGGCGACTTTCTAAGTCAAAATCTGGTGATTACGTTCTTTGTTGGGCAGATGGGTGTTGCAGAGAACCAAACGAAGAAGAAGGTGGAGTTAGAGAAACTCTGAGTCTTCGCATAGAAGAGGAAAAAGAAGAAGAAGAAGAAGAAGGAATGAAACAAAGTATGAAGAAGTGGGTTTTGCAGAAGTTGCATAAAGCTTTTGGTGGTTCAGATGAAGACAATTACGCTTTTGGCCTTGACCGTGTAACCGACACAGAGATGTTCTTTTTAGCTTCTATGTACTTCTTTTTCCCAAAAGGGTATGGTGGACCCGGTAAGTGTTTTGCTTCTGGTAAACATTTGTGGTTCAAATCTGTTTCTGATTATTGCTTTCGTTCTTTCTTGGCTAAATCTGCTGGAATTCAAACTGTTGTTTTAGTACCTACTGATTTTGGTGTTGTTGAGTTGGGTTCTGTTAGATTGTTACCTGAAATCTTTGATTTGTTGAACAAAATCAAATCATTGTTTTCAATTTCAAAACCTGCCACACAATCATTATCATTGTATCCTAGTGTGGTCAATGAAGAAAGTACAGTTTCAAATGGTGTTCAAGTTCCAAAAGTTGTGTTGAATTTGAACAATGGTCGTACACATTTTAGGGAGAAACTTGCCATTAGGAAAATGGATAGTAATAATGTTAATAATAATAGCATTAACTTTCCTAGTGCTAGAAATGGTATTTGTGGTTCTACTTGGGGAGTGAATCAAGGTCAAGGTGAATTTTTTGCTTCTAATGGTGTGAGAAAGGATTTTAGGCTCAACAACAATAATAGCAACTACCAACCACAAAGGCAGATGCCAATGCAAATTGACTTTTCCGGAGCAAATTCGAGGGCCAATTCTGCGAGACCGGTTATTGCTGAATCGGAACTTGTTGCTGATGTTGAGGCTGATCAACCTAGCGATGAAAGGAGACCTAGGAAGCGCGGAAGGAAGCCTGCTAATGGAAGGGACGAACCTCTAAATCATGTGGAGGCAGAGAGGCAAAGGCGTGAGAAGCTTAATCAGCGTTTTTACGCCTTAAGAGCTGTTGTACCGAATATTTCCAAGATGGACAAGGCATCTTTACTTGGTGATGCTATTGCTTACATCAATGAGCTTCAAGCGAAACTCAAGATCATGGAGTCAGAGAGAGAATCATTTGGAAGCAGTACTTCCAGGGATGGATCAGGCAACTCGAGATCAGAAAATCGTCATCAGAATACTAGAGTTTCTCTTCCGGATGTGGATATTCAAGCTTCTCAAGATGAGGTAATAGTAAAGGTCAGCTGTTCTCTTGATACTCATCCTGTTTCGAAACTAATCGAAACATTCAAAGAGGCACAAATCAGTGTTGTAGAGTCGAAACTTGCTGCTGCAAATGATACTATCTACCATAATTTTGTAATTAAGTCAGAAGGATCTGAACAGCTTACCAAGGACAAGTTGATTGCAGCATTTGCCCGAGAATCCAACTCCTTACAAACCCAAACTTTGTCCATATAG
Protein sequence

>MS.gene025894.t1

MGSIKRMIWNEEEKTMVGAILGARALDFLINNCLSNESGLIAVGESVDSLQNKLSDLVENSSSNFNWNYAIYWRLSKSKSGDYVLCWADGCCREPNEEEGGVRETLSLRIEEEKEEEEEEGMKQSMKKWVLQKLHKAFGGSDEDNYAFGLDRVTDTEMFFLASMYFFFPKGYGGPGKCFASGKHLWFKSVSDYCFRSFLAKSAGIQTVVLVPTDFGVVELGSVRLLPEIFDLLNKIKSLFSISKPATQSLSLYPSVVNEESTVSNGVQVPKVVLNLNNGRTHFREKLAIRKMDSNNVNNNSINFPSARNGICGSTWGVNQGQGEFFASNGVRKDFRLNNNNSNYQPQRQMPMQIDFSGANSRANSARPVIAESELVADVEADQPSDERRPRKRGRKPANGRDEPLNHVEAERQRREKLNQRFYALRAVVPNISKMDKASLLGDAIAYINELQAKLKIMESERESFGSSTSRDGSGNSRSENRHQNTRVSLPDVDIQASQDEVIVKVSCSLDTHPVSKLIETFKEAQISVVESKLAAANDTIYHNFVIKSEGSEQLTKDKLIAAFARESNSLQTQTLSI