AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026855

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026855.t1 MTR_2g076340 98.204 334 6 0 1 334 74 407 0.0 680
MS.gene026855.t1 MTR_3g072330 55.728 323 142 1 1 322 67 389 1.43e-129 375
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026855.t1 AT3G14930 81.155 329 62 0 1 329 78 406 0.0 570
MS.gene026855.t1 AT3G14930 81.155 329 62 0 1 329 78 406 0.0 570
MS.gene026855.t1 AT3G14930 81.155 329 62 0 1 329 1 329 0.0 569
MS.gene026855.t1 AT2G40490 53.870 323 148 1 1 322 72 394 3.42e-126 367

Find 92 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTTGTTCTTTATATAAA+TGG 0.110324 2.4:-21079285 MS.gene026855:CDS
GAACTTTCAGATTGTAAATT+TGG 0.179718 2.4:-21079331 MS.gene026855:intron
GACACTGGAGTTGATGTTAT+TGG 0.226105 2.4:-21079234 MS.gene026855:CDS
ACGCTCTGAGGAGGGATTAA+AGG 0.251035 2.4:-21080464 MS.gene026855:CDS
TAGTTTACCTCTTTGATATA+AGG 0.260471 2.4:+21080016 None:intergenic
TAAAGAACAAGTGGTGTATC+TGG 0.272940 2.4:+21079294 None:intergenic
ATACAGCACCACACATTCTA+AGG 0.323236 2.4:-21080174 MS.gene026855:CDS
GAACACCATGCCCAAGATTA+AGG 0.327102 2.4:+21078266 None:intergenic
ACCTCTGAATTTCTTCTGTT+AGG 0.334578 2.4:+21079106 None:intergenic
TAGAAATTTCTTTGCAGCCT+TGG 0.361660 2.4:-21080603 MS.gene026855:CDS
CTTGGGCATGGTGTTCTTGT+TGG 0.374900 2.4:-21078259 MS.gene026855:CDS
ACTCGACTGGACAGTGGATA+TGG 0.377098 2.4:-21079211 MS.gene026855:CDS
TGTATGGCACATGCTCTTAA+TGG 0.381907 2.4:+21080192 None:intergenic
GCATACAAATATTTGATTCA+TGG 0.385365 2.4:-21080081 MS.gene026855:CDS
GTTCTTTATATAAATGGAAA+TGG 0.386673 2.4:-21079279 MS.gene026855:CDS
TTCAGGGTTGTGAGGTCTGC+AGG 0.389800 2.4:-21078304 MS.gene026855:intron
CCTCTGAATTTCTTCTGTTA+GGG 0.395997 2.4:+21079107 None:intergenic
AGACAACACATCCTTAATCT+TGG 0.400361 2.4:-21078277 MS.gene026855:CDS
GAGAAAATAATTACTCCATC+AGG 0.416476 2.4:+21080568 None:intergenic
CATACAAATATTTGATTCAT+GGG 0.420865 2.4:-21080080 MS.gene026855:CDS
CGTATCGGTGACTGTATAAC+AGG 0.424760 2.4:+21080484 None:intergenic
CTAAGCTTGTCAAAGTCAAT+CGG 0.426657 2.4:+21080433 None:intergenic
GAAGAAGAAGATTAGGTAGC+GGG 0.442728 2.4:-21079182 MS.gene026855:CDS
TCTTCTGTTAGGGCAGTAAG+AGG 0.448836 2.4:+21079117 None:intergenic
CTTACACCTCTACCTGCATT+CGG 0.449149 2.4:-21080538 MS.gene026855:CDS
AGAAGATTAGGTAGCGGGAT+TGG 0.454956 2.4:-21079177 MS.gene026855:CDS
AAATGTGCAACAGCTTCTTC+AGG 0.457455 2.4:+21078232 None:intergenic
TTACACCTCTACCTGCATTC+GGG 0.464392 2.4:-21080537 MS.gene026855:CDS
GACAACACATCCTTAATCTT+GGG 0.466437 2.4:-21078276 MS.gene026855:CDS
GACAACTGCCGCCTCATATG+TGG 0.470143 2.4:-21080054 MS.gene026855:CDS
TTAGAATGTGTGGTGCTGTA+TGG 0.470252 2.4:+21080176 None:intergenic
GACCAGAGTTCCCACATATG+AGG 0.473769 2.4:+21080043 None:intergenic
GCTGATGGAAGAAGAAGATT+AGG 0.476250 2.4:-21079189 MS.gene026855:CDS
TGTGGGAGCACCTTGGACAT+TGG 0.478006 2.4:-21080257 MS.gene026855:CDS
GACATTGGCAACGTATATAG+TGG 0.481356 2.4:-21080242 MS.gene026855:CDS
GGCAACGTATATAGTGGAAG+GGG 0.484531 2.4:-21080236 MS.gene026855:CDS
CAGTCGTCTCTGACCTCTCT+CGG 0.502489 2.4:+21080635 None:intergenic
AGAGGGGAGAATAAAGAAGC+AGG 0.503530 2.4:+21079135 None:intergenic
CGCCTCATATGTGGGAACTC+TGG 0.505272 2.4:-21080045 MS.gene026855:CDS
TCTGACCTCTCTCGGAAGGA+TGG 0.513347 2.4:+21080643 None:intergenic
GCATGGATGATGCGCCAAGC+AGG 0.516013 2.4:-21080706 None:intergenic
CACATTTCTTTGATGTCGCG+AGG 0.528865 2.4:-21078216 MS.gene026855:CDS
CAAATATTTGATTCATGGGG+TGG 0.529717 2.4:-21080076 MS.gene026855:CDS
TCTGCTTTCTCATTTGTCAA+AGG 0.532489 2.4:-21080149 MS.gene026855:CDS
TTGTCACTTTCAGGGTTGTG+AGG 0.534765 2.4:-21078312 MS.gene026855:intron
CTTCTGTTAGGGCAGTAAGA+GGG 0.535185 2.4:+21079118 None:intergenic
AAAGAACAAGTGGTGTATCT+GGG 0.541095 2.4:+21079295 None:intergenic
TAGCCATGTACCTTCCTGCT+TGG 0.546091 2.4:+21080692 None:intergenic
TTCTGTTAGGGCAGTAAGAG+GGG 0.546842 2.4:+21079119 None:intergenic
TAAGCTTGTCAAAGTCAATC+GGG 0.548224 2.4:+21080434 None:intergenic
AGATTAAGGATGTGTTGTCT+AGG 0.548882 2.4:+21078280 None:intergenic
GGAAGAAGAAGATTAGGTAG+CGG 0.558618 2.4:-21079183 MS.gene026855:CDS
TCGATCCCGAATGCAGGTAG+AGG 0.560715 2.4:+21080532 None:intergenic
CGAATGCAGGTAGAGGTGTA+AGG 0.561238 2.4:+21080539 None:intergenic
TCAAAGTCGATCCCGAATGC+AGG 0.561442 2.4:+21080526 None:intergenic
CGTCTCTGACCTCTCTCGGA+AGG 0.569014 2.4:+21080639 None:intergenic
CTTTGACATAGAAGATGTGA+GGG 0.572515 2.4:-21080509 MS.gene026855:CDS
GCGCCAAGCAGGAAGGTACA+TGG 0.572903 2.4:-21080695 MS.gene026855:CDS
AACATCCATCCTTCCGAGAG+AGG 0.577108 2.4:-21080648 MS.gene026855:CDS
AATCCCTCCTCAGAGCGTAT+CGG 0.581435 2.4:+21080469 None:intergenic
TTGGCAACGTATATAGTGGA+AGG 0.583350 2.4:-21080238 MS.gene026855:CDS
TTGATGTTATTGGACTCGAC+TGG 0.583463 2.4:-21079224 MS.gene026855:CDS
CTTTATATAAATGGAAATGG+TGG 0.584814 2.4:-21079276 MS.gene026855:CDS
AGCAGAGTCCTTAGAATGTG+TGG 0.585181 2.4:+21080166 None:intergenic
CCATTTATATAAAGAACAAG+TGG 0.585667 2.4:+21079285 None:intergenic
ACAACTGCCGCCTCATATGT+GGG 0.588080 2.4:-21080053 MS.gene026855:CDS
ACTTAAGATCCTGCGGCAAG+AGG 0.593099 2.4:-21080395 MS.gene026855:intron
GTCAAAGCCTTATATCAAAG+AGG 0.596608 2.4:-21080023 MS.gene026855:intron
CACATCCTTAATCTTGGGCA+TGG 0.600175 2.4:-21078271 MS.gene026855:CDS
ATATACGTTGCCAATGTCCA+AGG 0.600785 2.4:+21080247 None:intergenic
TGGCAACGTATATAGTGGAA+GGG 0.603156 2.4:-21080237 MS.gene026855:CDS
TGGACAGTGGATATGGCTGA+TGG 0.608369 2.4:-21079204 MS.gene026855:CDS
TCACCGATACGCTCTGAGGA+GGG 0.608994 2.4:-21080472 MS.gene026855:CDS
GTCACCGATACGCTCTGAGG+AGG 0.616864 2.4:-21080473 MS.gene026855:CDS
ACTTTGACATAGAAGATGTG+AGG 0.620333 2.4:-21080510 MS.gene026855:CDS
GATTGGTGTGCAAGGAAACG+TGG 0.621871 2.4:-21079160 MS.gene026855:CDS
TGTCTATCATACGCTCGAGA+AGG 0.625970 2.4:+21079253 None:intergenic
TCAGGCCTAAAAGCTTTCCA+AGG 0.632316 2.4:+21080586 None:intergenic
CTCGAGCGTATGATAGACAC+TGG 0.632830 2.4:-21079249 MS.gene026855:CDS
AGTGAGCCCCAGATTCCACT+TGG 0.635817 2.4:+21080104 None:intergenic
GGTAGCGGGATTGGTGTGCA+AGG 0.635971 2.4:-21079168 MS.gene026855:CDS
GAGGGGAGAATAAAGAAGCA+GGG 0.636691 2.4:+21079136 None:intergenic
GGATGATGCGCCAAGCAGGA+AGG 0.641154 2.4:-21080702 MS.gene026855:CDS
TCCCACAAAACCTAAAACAG+CGG 0.645378 2.4:+21080273 None:intergenic
ATACAAATATTTGATTCATG+GGG 0.650037 2.4:-21080079 MS.gene026855:CDS
CCCTAACAGAAGAAATTCAG+AGG 0.651466 2.4:-21079107 MS.gene026855:intron
CAGAGTTCCCACATATGAGG+CGG 0.665889 2.4:+21080046 None:intergenic
GAGAATCACTTAAGATCCTG+CGG 0.672224 2.4:-21080402 MS.gene026855:CDS
TATTGGACTCGACTGGACAG+TGG 0.685653 2.4:-21079217 MS.gene026855:CDS
ACAGTCACCGATACGCTCTG+AGG 0.696424 2.4:-21080476 MS.gene026855:CDS
GCAACGTATATAGTGGAAGG+GGG 0.725564 2.4:-21080235 MS.gene026855:CDS
TTTGACATAGAAGATGTGAG+GGG 0.768540 2.4:-21080508 MS.gene026855:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATAAATAGAAAATTCT+AGG - chr2.4:21079457-21079476 MS.gene026855:intron 10.0%
!! CATATTGTTTATATTTCATT+GGG - chr2.4:21079400-21079419 MS.gene026855:intron 15.0%
!! TCATATTGTTTATATTTCAT+TGG - chr2.4:21079399-21079418 MS.gene026855:intron 15.0%
!! TTAGTTTCAATATTATGAAT+TGG - chr2.4:21080335-21080354 MS.gene026855:intron 15.0%
!! ATACAAATATTTGATTCATG+GGG - chr2.4:21078786-21078805 MS.gene026855:intron 20.0%
!! CATACAAATATTTGATTCAT+GGG - chr2.4:21078785-21078804 MS.gene026855:intron 20.0%
!! GTAATATAAGAAATGAGAAA+AGG + chr2.4:21079507-21079526 None:intergenic 20.0%
!! GTTCTTTATATAAATGGAAA+TGG - chr2.4:21079586-21079605 MS.gene026855:intron 20.0%
!!! AAACCTAAAACAATTTTATC+AGG + chr2.4:21078900-21078919 None:intergenic 20.0%
!!! AATTTCTTTTGTTGAAGAAA+GGG - chr2.4:21078953-21078972 MS.gene026855:intron 20.0%
!!! ATACCTGATAAAATTGTTTT+AGG - chr2.4:21078894-21078913 MS.gene026855:intron 20.0%
! AAAAAAACGATAAGTAGTGA+GGG + chr2.4:21078557-21078576 None:intergenic 25.0%
! AGTGATAGAAATGTTTCTTA+AGG - chr2.4:21080288-21080307 MS.gene026855:CDS 25.0%
! ATATCAAAGAGGTAAACTAA+AGG - chr2.4:21078853-21078872 MS.gene026855:intron 25.0%
! CCACTTGTTCTTTATATAAA+TGG - chr2.4:21079580-21079599 MS.gene026855:intron 25.0%
! CCATTTATATAAAGAACAAG+TGG + chr2.4:21079583-21079602 None:intergenic 25.0%
! CTTTATATAAATGGAAATGG+TGG - chr2.4:21079589-21079608 MS.gene026855:intron 25.0%
! CTTTCTTCAACAAAAGAAAT+TGG + chr2.4:21078954-21078973 None:intergenic 25.0%
! GAACTTTCAGATTGTAAATT+TGG - chr2.4:21079534-21079553 MS.gene026855:intron 25.0%
! GCATACAAATATTTGATTCA+TGG - chr2.4:21078784-21078803 MS.gene026855:intron 25.0%
! TAAAAAAACGATAAGTAGTG+AGG + chr2.4:21078558-21078577 None:intergenic 25.0%
! TAAACAAAAAAGATGCAGAA+AGG + chr2.4:21080170-21080189 None:intergenic 25.0%
! TAGTTTACCTCTTTGATATA+AGG + chr2.4:21078852-21078871 None:intergenic 25.0%
! TTATCAGGTATGAAAGAAAT+GGG + chr2.4:21078885-21078904 None:intergenic 25.0%
! TTTATCAGGTATGAAAGAAA+TGG + chr2.4:21078886-21078905 None:intergenic 25.0%
!! CAATTTCTTTTGTTGAAGAA+AGG - chr2.4:21078952-21078971 MS.gene026855:intron 25.0%
!! TTGTAAATTTTTGCTGCAAA+TGG - chr2.4:21079266-21079285 MS.gene026855:CDS 25.0%
!!! TACTTATCGTTTTTTTAGGT+TGG - chr2.4:21078561-21078580 MS.gene026855:intron 25.0%
!!! TCACTACTTATCGTTTTTTT+AGG - chr2.4:21078557-21078576 MS.gene026855:intron 25.0%
AACATCAGTTTCTCATTATG+TGG + chr2.4:21080100-21080119 None:intergenic 30.0%
AGTACAATGTGTTCAAATAG+TGG + chr2.4:21079947-21079966 None:intergenic 30.0%
AGTCAGTTAAGCAAATACTA+GGG + chr2.4:21080228-21080247 None:intergenic 30.0%
ATGAGCTTACATAATAGTCA+AGG + chr2.4:21079313-21079332 None:intergenic 30.0%
ATGTTGTCAATTGCATATAG+TGG + chr2.4:21080038-21080057 None:intergenic 30.0%
CTCATTGTGATGAATAAGAT+TGG - chr2.4:21080475-21080494 MS.gene026855:CDS 30.0%
GAAATGAGAAAAGGAGAAAT+TGG + chr2.4:21079498-21079517 None:intergenic 30.0%
GACACGAATGATAAAGTTAA+TGG + chr2.4:21079799-21079818 None:intergenic 30.0%
GAGAAAATAATTACTCCATC+AGG + chr2.4:21078300-21078319 None:intergenic 30.0%
GTTATTGCCATATATGTTCT+AGG - chr2.4:21079830-21079849 MS.gene026855:intron 30.0%
TATCAGGTATGAAAGAAATG+GGG + chr2.4:21078884-21078903 None:intergenic 30.0%
! AGATTACATTGTTTTCCAAG+TGG - chr2.4:21078746-21078765 MS.gene026855:intron 30.0%
!! ATGATTACTTGTCACTTTCA+GGG - chr2.4:21080545-21080564 MS.gene026855:CDS 30.0%
!! TATGATTACTTGTCACTTTC+AGG - chr2.4:21080544-21080563 MS.gene026855:CDS 30.0%
!!! TTATCGTTTTTTTAGGTTGG+TGG - chr2.4:21078564-21078583 MS.gene026855:intron 30.0%
!!! TTTGACAAGCTTAGTTTTGT+AGG - chr2.4:21078441-21078460 MS.gene026855:intron 30.0%
AAAATTCGGTTGCAACATGT+CGG - chr2.4:21078494-21078513 MS.gene026855:intron 35.0%
AAAGAACAAGTGGTGTATCT+GGG + chr2.4:21079573-21079592 None:intergenic 35.0%
AACAGAAGAAATTCAGAGGT+TGG - chr2.4:21079762-21079781 MS.gene026855:intron 35.0%
AACTGAAATTAGTGACACGA+AGG - chr2.4:21080252-21080271 MS.gene026855:CDS 35.0%
ACCTCTGAATTTCTTCTGTT+AGG + chr2.4:21079762-21079781 None:intergenic 35.0%
ACTGAAATTAGTGACACGAA+GGG - chr2.4:21080253-21080272 MS.gene026855:CDS 35.0%
ACTTTGACATAGAAGATGTG+AGG - chr2.4:21078355-21078374 MS.gene026855:intron 35.0%
AGACAACACATCCTTAATCT+TGG - chr2.4:21080588-21080607 MS.gene026855:CDS 35.0%
AGATTAAGGATGTGTTGTCT+AGG + chr2.4:21080588-21080607 None:intergenic 35.0%
AGTCTCTCCTAGAACATATA+TGG + chr2.4:21079840-21079859 None:intergenic 35.0%
CAAATATTTGATTCATGGGG+TGG - chr2.4:21078789-21078808 MS.gene026855:intron 35.0%
CACCATGTCATACAAAACTT+TGG + chr2.4:21080398-21080417 None:intergenic 35.0%
CAGTCAGTTAAGCAAATACT+AGG + chr2.4:21080229-21080248 None:intergenic 35.0%
CCTCTGAATTTCTTCTGTTA+GGG + chr2.4:21079761-21079780 None:intergenic 35.0%
CTAAATTAGCCCACCTAAAT+TGG + chr2.4:21079131-21079150 None:intergenic 35.0%
CTAAGCTTGTCAAAGTCAAT+CGG + chr2.4:21078435-21078454 None:intergenic 35.0%
CTCATTATGTGGAATCATCA+AGG + chr2.4:21080089-21080108 None:intergenic 35.0%
CTTTGACATAGAAGATGTGA+GGG - chr2.4:21078356-21078375 MS.gene026855:intron 35.0%
GACAACACATCCTTAATCTT+GGG - chr2.4:21080589-21080608 MS.gene026855:CDS 35.0%
GTATACGCTATAGCTGTATT+TGG + chr2.4:21080002-21080021 None:intergenic 35.0%
TAAAGAACAAGTGGTGTATC+TGG + chr2.4:21079574-21079593 None:intergenic 35.0%
TAAGCTTGTCAAAGTCAATC+GGG + chr2.4:21078434-21078453 None:intergenic 35.0%
TAGAAATTTCTTTGCAGCCT+TGG - chr2.4:21078262-21078281 MS.gene026855:CDS 35.0%
TAGTATATACGTAACCCACT+TGG - chr2.4:21079030-21079049 MS.gene026855:intron 35.0%
TATGATTCCTCTTGGTTAGT+TGG - chr2.4:21079196-21079215 MS.gene026855:CDS 35.0%
TCAATTGCATATAGTGGCTA+TGG + chr2.4:21080032-21080051 None:intergenic 35.0%
TCTGCTTTCTCATTTGTCAA+AGG - chr2.4:21078716-21078735 MS.gene026855:intron 35.0%
TGGTTTAATCAGTCAGACTT+GGG - chr2.4:21080355-21080374 MS.gene026855:intron 35.0%
TTGGTTTAATCAGTCAGACT+TGG - chr2.4:21080354-21080373 MS.gene026855:intron 35.0%
TTTCTGAGCTCTGTATAGTT+AGG - chr2.4:21078989-21079008 MS.gene026855:intron 35.0%
TTTGACATAGAAGATGTGAG+GGG - chr2.4:21078357-21078376 MS.gene026855:intron 35.0%
! ATACAAAACTTTGGCCTTTC+TGG + chr2.4:21080389-21080408 None:intergenic 35.0%
! ATTGTTTTCCAAGTGGAATC+TGG - chr2.4:21078753-21078772 MS.gene026855:intron 35.0%
! GTCAAAGCCTTATATCAAAG+AGG - chr2.4:21078842-21078861 MS.gene026855:intron 35.0%
! TTGTTTTCCAAGTGGAATCT+GGG - chr2.4:21078754-21078773 MS.gene026855:intron 35.0%
!! GGGTTGGTAAATAATTTGAC+TGG - chr2.4:21079229-21079248 MS.gene026855:CDS 35.0%
!! TGTCGGCACTTTTTATATCA+TGG - chr2.4:21078511-21078530 MS.gene026855:intron 35.0%
AAATGTGCAACAGCTTCTTC+AGG + chr2.4:21080636-21080655 None:intergenic 40.0%
ATACAGCACCACACATTCTA+AGG - chr2.4:21078691-21078710 MS.gene026855:intron 40.0%
ATATACGTTGCCAATGTCCA+AGG + chr2.4:21078621-21078640 None:intergenic 40.0%
ATTCTCTCCAGTGCCAATTT+AGG - chr2.4:21079115-21079134 MS.gene026855:CDS 40.0%
CAAGAGGAATCATAGAGACT+TGG + chr2.4:21079190-21079209 None:intergenic 40.0%
CAAGTCTCTATGATTCCTCT+TGG - chr2.4:21079188-21079207 MS.gene026855:CDS 40.0%
CAGTTAAGCAAATACTAGGG+AGG + chr2.4:21080225-21080244 None:intergenic 40.0%
CCCTAACAGAAGAAATTCAG+AGG - chr2.4:21079758-21079777 MS.gene026855:intron 40.0%
GAAACTGATGTTTCATCACG+TGG - chr2.4:21080108-21080127 MS.gene026855:CDS 40.0%
GAAGAAGAAGATTAGGTAGC+GGG - chr2.4:21079683-21079702 MS.gene026855:intron 40.0%
GACATTGGCAACGTATATAG+TGG - chr2.4:21078623-21078642 MS.gene026855:intron 40.0%
GAGAATCACTTAAGATCCTG+CGG - chr2.4:21078463-21078482 MS.gene026855:intron 40.0%
GAGTTGTCAATTGCAGATCA+TGG - chr2.4:21079869-21079888 MS.gene026855:intron 40.0%
GCTGATGGAAGAAGAAGATT+AGG - chr2.4:21079676-21079695 MS.gene026855:intron 40.0%
GGAAGAAGAAGATTAGGTAG+CGG - chr2.4:21079682-21079701 MS.gene026855:intron 40.0%
GTGGTGATAAATGCATTACC+AGG - chr2.4:21080127-21080146 MS.gene026855:CDS 40.0%
TATACGTAACCCACTTGGAT+TGG - chr2.4:21079035-21079054 MS.gene026855:intron 40.0%
TCACAATGAGTGTTCGCATT+TGG + chr2.4:21080465-21080484 None:intergenic 40.0%
TCATTCGTGTCGTCATTCTA+TGG - chr2.4:21079808-21079827 MS.gene026855:intron 40.0%
TCCCACAAAACCTAAAACAG+CGG + chr2.4:21078595-21078614 None:intergenic 40.0%
TCTGCAATTGACAACTCAAG+TGG + chr2.4:21079866-21079885 None:intergenic 40.0%
TGTATGGCACATGCTCTTAA+TGG + chr2.4:21078676-21078695 None:intergenic 40.0%
TTGATGTTATTGGACTCGAC+TGG - chr2.4:21079641-21079660 MS.gene026855:intron 40.0%
TTGCAGATCATGGAAAGTAG+CGG - chr2.4:21079879-21079898 MS.gene026855:intron 40.0%
! ATTCCTCTTGGTTAGTTGGT+TGG - chr2.4:21079200-21079219 MS.gene026855:CDS 40.0%
! TGGCAACGTATATAGTGGAA+GGG - chr2.4:21078628-21078647 MS.gene026855:intron 40.0%
! TGTTTTCCAAGTGGAATCTG+GGG - chr2.4:21078755-21078774 MS.gene026855:intron 40.0%
! TTGGCAACGTATATAGTGGA+AGG - chr2.4:21078627-21078646 MS.gene026855:intron 40.0%
!! GACACTGGAGTTGATGTTAT+TGG - chr2.4:21079631-21079650 MS.gene026855:intron 40.0%
!! GGCCAAAGTTTTGTATGACA+TGG - chr2.4:21080393-21080412 MS.gene026855:intron 40.0%
!! TTAGAATGTGTGGTGCTGTA+TGG + chr2.4:21078692-21078711 None:intergenic 40.0%
AGAGGGGAGAATAAAGAAGC+AGG + chr2.4:21079733-21079752 None:intergenic 45.0%
AGCAGAGTCCTTAGAATGTG+TGG + chr2.4:21078702-21078721 None:intergenic 45.0%
ATATAGTGGCTATGGCGTAG+CGG + chr2.4:21080024-21080043 None:intergenic 45.0%
ATCATCAAGGAGAGTCGCTT+AGG + chr2.4:21080076-21080095 None:intergenic 45.0%
ATGCAGAAAGGACATTCGCT+AGG + chr2.4:21080158-21080177 None:intergenic 45.0%
CACATCCTTAATCTTGGGCA+TGG - chr2.4:21080594-21080613 MS.gene026855:CDS 45.0%
CACATTTCTTTGATGTCGCG+AGG - chr2.4:21080649-21080668 MS.gene026855:CDS 45.0%
CTGCGGCAAGAGGTAAAATT+CGG - chr2.4:21078480-21078499 MS.gene026855:intron 45.0%
CTTACACCTCTACCTGCATT+CGG - chr2.4:21078327-21078346 MS.gene026855:intron 45.0%
CTTCTGTTAGGGCAGTAAGA+GGG + chr2.4:21079750-21079769 None:intergenic 45.0%
GAACACCATGCCCAAGATTA+AGG + chr2.4:21080602-21080621 None:intergenic 45.0%
GAGGGGAGAATAAAGAAGCA+GGG + chr2.4:21079732-21079751 None:intergenic 45.0%
GATACACGCATCTTAGACAC+TGG + chr2.4:21080197-21080216 None:intergenic 45.0%
TAAGCGTGCTTGTGACTTCA+AGG - chr2.4:21079340-21079359 MS.gene026855:intron 45.0%
TCAGGCCTAAAAGCTTTCCA+AGG + chr2.4:21078282-21078301 None:intergenic 45.0%
TCTCCAGTGCCAATTTAGGT+GGG - chr2.4:21079119-21079138 MS.gene026855:CDS 45.0%
TCTTCTGTTAGGGCAGTAAG+AGG + chr2.4:21079751-21079770 None:intergenic 45.0%
TGTCTATCATACGCTCGAGA+AGG + chr2.4:21079615-21079634 None:intergenic 45.0%
TTACACCTCTACCTGCATTC+GGG - chr2.4:21078328-21078347 MS.gene026855:intron 45.0%
TTCTGTTAGGGCAGTAAGAG+GGG + chr2.4:21079749-21079768 None:intergenic 45.0%
! AACTTTGGCCTTTCTGGCAA+AGG + chr2.4:21080383-21080402 None:intergenic 45.0%
! CGTATCGGTGACTGTATAAC+AGG + chr2.4:21078384-21078403 None:intergenic 45.0%
! GCAACGTATATAGTGGAAGG+GGG - chr2.4:21078630-21078649 MS.gene026855:intron 45.0%
! GGCAACGTATATAGTGGAAG+GGG - chr2.4:21078629-21078648 MS.gene026855:intron 45.0%
! TGCAGCCTTGGAAAGCTTTT+AGG - chr2.4:21078274-21078293 MS.gene026855:CDS 45.0%
! TGGAAAGCTTTTAGGCCTGA+TGG - chr2.4:21078282-21078301 MS.gene026855:CDS 45.0%
!! AGAAGATTAGGTAGCGGGAT+TGG - chr2.4:21079688-21079707 MS.gene026855:intron 45.0%
!! TTGTCACTTTCAGGGTTGTG+AGG - chr2.4:21080553-21080572 MS.gene026855:CDS 45.0%
!!! GCCGCTGTTTTAGGTTTTGT+GGG - chr2.4:21078591-21078610 MS.gene026855:intron 45.0%
!!! TGCCGCTGTTTTAGGTTTTG+TGG - chr2.4:21078590-21078609 MS.gene026855:intron 45.0%
AACATCCATCCTTCCGAGAG+AGG - chr2.4:21078217-21078236 MS.gene026855:CDS 50.0%
AATCCCTCCTCAGAGCGTAT+CGG + chr2.4:21078399-21078418 None:intergenic 50.0%
ACAACTGCCGCCTCATATGT+GGG - chr2.4:21078812-21078831 MS.gene026855:intron 50.0%
ACTCGACTGGACAGTGGATA+TGG - chr2.4:21079654-21079673 MS.gene026855:intron 50.0%
ACTTAAGATCCTGCGGCAAG+AGG - chr2.4:21078470-21078489 MS.gene026855:intron 50.0%
CAGAGTTCCCACATATGAGG+CGG + chr2.4:21078822-21078841 None:intergenic 50.0%
CAGCCAACCAACTAACCAAG+AGG + chr2.4:21079206-21079225 None:intergenic 50.0%
CTCGAGCGTATGATAGACAC+TGG - chr2.4:21079616-21079635 MS.gene026855:intron 50.0%
CTCTCCAGTGCCAATTTAGG+TGG - chr2.4:21079118-21079137 MS.gene026855:CDS 50.0%
CTTGGGCATGGTGTTCTTGT+TGG - chr2.4:21080606-21080625 MS.gene026855:CDS 50.0%
GAATCGAACATGAGACCACG+AGG + chr2.4:21079099-21079118 None:intergenic 50.0%
GACATTCGCTAGGAAGATCC+TGG + chr2.4:21080148-21080167 None:intergenic 50.0%
GACCAGAGTTCCCACATATG+AGG + chr2.4:21078825-21078844 None:intergenic 50.0%
GAGTCGCTTAGGAACTACTC+TGG + chr2.4:21080065-21080084 None:intergenic 50.0%
GATTGGTGTGCAAGGAAACG+TGG - chr2.4:21079705-21079724 MS.gene026855:intron 50.0%
TAGCCATGTACCTTCCTGCT+TGG + chr2.4:21078176-21078195 None:intergenic 50.0%
TAGCCCACCTAAATTGGCAC+TGG + chr2.4:21079125-21079144 None:intergenic 50.0%
TATTGGACTCGACTGGACAG+TGG - chr2.4:21079648-21079667 MS.gene026855:intron 50.0%
TCAAAGTCGATCCCGAATGC+AGG + chr2.4:21078342-21078361 None:intergenic 50.0%
TGGACAGTGGATATGGCTGA+TGG - chr2.4:21079661-21079680 MS.gene026855:intron 50.0%
! CGAATGCAGGTAGAGGTGTA+AGG + chr2.4:21078329-21078348 None:intergenic 50.0%
! CGAATTTTACCTCTTGCCGC+AGG + chr2.4:21078482-21078501 None:intergenic 50.0%
! CTCTTGGTTAGTTGGTTGGC+TGG - chr2.4:21079204-21079223 MS.gene026855:CDS 50.0%
!! ACGCTCTGAGGAGGGATTAA+AGG - chr2.4:21078401-21078420 MS.gene026855:intron 50.0%
!! GTGGTATTGACTTGGGACCT+GGG - chr2.4:21079062-21079081 MS.gene026855:intron 50.0%
!! TGGCCTGGTGGTATTGACTT+GGG - chr2.4:21079055-21079074 MS.gene026855:intron 50.0%
!! TTGGCCTGGTGGTATTGACT+TGG - chr2.4:21079054-21079073 MS.gene026855:intron 50.0%
!!! TAGGTTTTGTGGGAGCACCT+TGG - chr2.4:21078601-21078620 MS.gene026855:intron 50.0%
ACAGTCACCGATACGCTCTG+AGG - chr2.4:21078389-21078408 MS.gene026855:intron 55.0%
ACCACCAGGCCAATCCAAGT+GGG + chr2.4:21079047-21079066 None:intergenic 55.0%
AGTGAGCCCCAGATTCCACT+TGG + chr2.4:21078764-21078783 None:intergenic 55.0%
CAGTCGTCTCTGACCTCTCT+CGG + chr2.4:21078233-21078252 None:intergenic 55.0%
CGCCTCATATGTGGGAACTC+TGG - chr2.4:21078820-21078839 MS.gene026855:intron 55.0%
GACAACTGCCGCCTCATATG+TGG - chr2.4:21078811-21078830 MS.gene026855:intron 55.0%
GGTCCCAAGTCAATACCACC+AGG + chr2.4:21079061-21079080 None:intergenic 55.0%
TACCACCAGGCCAATCCAAG+TGG + chr2.4:21079048-21079067 None:intergenic 55.0%
TCGAACATGAGACCACGAGG+AGG + chr2.4:21079096-21079115 None:intergenic 55.0%
TCGATCCCGAATGCAGGTAG+AGG + chr2.4:21078336-21078355 None:intergenic 55.0%
TCTGACCTCTCTCGGAAGGA+TGG + chr2.4:21078225-21078244 None:intergenic 55.0%
TTCAGGGTTGTGAGGTCTGC+AGG - chr2.4:21080561-21080580 MS.gene026855:CDS 55.0%
! GGTTAGTTGGTTGGCTGGCT+GGG - chr2.4:21079209-21079228 MS.gene026855:CDS 55.0%
! TGGTTAGTTGGTTGGCTGGC+TGG - chr2.4:21079208-21079227 MS.gene026855:CDS 55.0%
!! GGTGGTATTGACTTGGGACC+TGG - chr2.4:21079061-21079080 MS.gene026855:intron 55.0%
!! GGTGGTGATGCCGCTGTTTT+AGG - chr2.4:21078582-21078601 MS.gene026855:intron 55.0%
!! GTAACCCACTTGGATTGGCC+TGG - chr2.4:21079040-21079059 MS.gene026855:intron 55.0%
!! TCACCGATACGCTCTGAGGA+GGG - chr2.4:21078393-21078412 MS.gene026855:intron 55.0%
!! TGTGGGAGCACCTTGGACAT+TGG - chr2.4:21078608-21078627 MS.gene026855:intron 55.0%
CGTCTCTGACCTCTCTCGGA+AGG + chr2.4:21078229-21078248 None:intergenic 60.0%
CTTGGGCGCCTTTGCCAGAA+AGG - chr2.4:21080372-21080391 MS.gene026855:intron 60.0%
! AGTTGGTTGGCTGGCTGGGT+TGG - chr2.4:21079213-21079232 MS.gene026855:CDS 60.0%
! GCGCCAAGCAGGAAGGTACA+TGG - chr2.4:21078170-21078189 MS.gene026855:CDS 60.0%
!! ACCCACTTGGATTGGCCTGG+TGG - chr2.4:21079043-21079062 MS.gene026855:intron 60.0%
!! GGTAGCGGGATTGGTGTGCA+AGG - chr2.4:21079697-21079716 MS.gene026855:intron 60.0%
!! GTCACCGATACGCTCTGAGG+AGG - chr2.4:21078392-21078411 MS.gene026855:intron 60.0%
! ACGAGGAGGAGCACACTCCC+AGG + chr2.4:21079082-21079101 None:intergenic 65.0%
! TGGGAGTGTGCTCCTCCTCG+TGG - chr2.4:21079081-21079100 MS.gene026855:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 21078165 21080722 21078165 ID=MS.gene026855
chr2.4 mRNA 21078165 21080722 21078165 ID=MS.gene026855.t1;Parent=MS.gene026855
chr2.4 exon 21080396 21080722 21080396 ID=MS.gene026855.t1.exon1;Parent=MS.gene026855.t1
chr2.4 CDS 21080396 21080722 21080396 ID=cds.MS.gene026855.t1;Parent=MS.gene026855.t1
chr2.4 exon 21080024 21080308 21080024 ID=MS.gene026855.t1.exon2;Parent=MS.gene026855.t1
chr2.4 CDS 21080024 21080308 21080024 ID=cds.MS.gene026855.t1;Parent=MS.gene026855.t1
chr2.4 exon 21079108 21079343 21079108 ID=MS.gene026855.t1.exon3;Parent=MS.gene026855.t1
chr2.4 CDS 21079108 21079343 21079108 ID=cds.MS.gene026855.t1;Parent=MS.gene026855.t1
chr2.4 exon 21078165 21078321 21078165 ID=MS.gene026855.t1.exon4;Parent=MS.gene026855.t1
chr2.4 CDS 21078165 21078321 21078165 ID=cds.MS.gene026855.t1;Parent=MS.gene026855.t1
Gene Sequence

>MS.gene026855

ATGATGCGCCAAGCAGGAAGGTACATGGCTATTTACAGAAAGTTAGCTGAGAAACATCCATCCTTCCGAGAGAGGTCAGAGACGACTGATCTCATTGTAGAAATTTCTTTGCAGCCTTGGAAAGCTTTTAGGCCTGATGGAGTAATTATTTTCTCTGACATCCTTACACCTCTACCTGCATTCGGGATCGACTTTGACATAGAAGATGTGAGGGGACCTGTTATACAGTCACCGATACGCTCTGAGGAGGGATTAAAGGTTTTACACCCGATTGACTTTGACAAGCTTAGTTTTGTAGGAGAATCACTTAAGATCCTGCGGCAAGAGGTAAAATTCGGTTGCAACATGTCGGCACTTTTTATATCATGGCTATCTTTCTAAGCATGTTTCCCTCACTACTTATCGTTTTTTTAGGTTGGTGGTGATGCCGCTGTTTTAGGTTTTGTGGGAGCACCTTGGACATTGGCAACGTATATAGTGGAAGGGGGTACGACACGTACATATACAACCATTAAGAGCATGTGCCATACAGCACCACACATTCTAAGGACTCTGCTTTCTCATTTGTCAAAGGCGATAGCAGATTACATTGTTTTCCAAGTGGAATCTGGGGCTCACTGCATACAAATATTTGATTCATGGGGTGGACAACTGCCGCCTCATATGTGGGAACTCTGGTCAAAGCCTTATATCAAAGAGGTAAACTAAAGGAAAATCCCCATTTCTTTCATACCTGATAAAATTGTTTTAGGTTTTTAAAGTTTTCATTATCAAGTCTAATTATAACCAATTTCTTTTGTTGAAGAAAGGGAATAGCATGTAGCTTTCTGAGCTCTGTATAGTTAGGTTGATGTCAAATTAATTATAGTATATACGTAACCCACTTGGATTGGCCTGGTGGTATTGACTTGGGACCTGGGAGTGTGCTCCTCCTCGTGGTCTCATGTTCGATTCTCTCCAGTGCCAATTTAGGTGGGCTAATTTAGCTTCTTCAAAAAAAAAAAATTATAGTGTATACGTTTCCAAGTCTCTATGATTCCTCTTGGTTAGTTGGTTGGCTGGCTGGGTTGGTAAATAATTTGACTGGTAAAAGCTTGTAATTTGTAAATTTTTGCTGCAAATGGAAAATACTTGTTATGTGTAAGCCTTGACTATTATGTAAGCTCATTCGATATTAAGCGTGCTTGTGACTTCAAGGAATAACATTTTAAATTAGAACTCAGAAGCTACATCTTCATATTGTTTATATTTCATTGGGATAATTATTGCTATTAGTATCTTGTTTAAAAAATTATAATAAATAGAAAATTCTAGGTTGACTTCTGTTGTGCCAATTTCTCCTTTTCTCATTTCTTATATTACACTTTCTGAACTTTCAGATTGTAAATTTGGTCAAGAAAAAGTGCCCAGATACACCACTTGTTCTTTATATAAATGGAAATGGTGGCCTTCTCGAGCGTATGATAGACACTGGAGTTGATGTTATTGGACTCGACTGGACAGTGGATATGGCTGATGGAAGAAGAAGATTAGGTAGCGGGATTGGTGTGCAAGGAAACGTGGACCCTGCTTCTTTATTCTCCCCTCTTACTGCCCTAACAGAAGAAATTCAGAGGTTGGTGACATAACTGCCATTAACTTTATCATTCGTGTCGTCATTCTATGGTTATTGCCATATATGTTCTAGGAGAGACTACACCACTTGAGTTGTCAATTGCAGATCATGGAAAGTAGCGGTTTGTTCAGATATCACTATGCTATAGCGCTGTTGTACTATAGCCACTATTTGAACACATTGTACTAAATAACAAATCAGAAATCAATAATATTTTGTCCAAATACAGCTATAGCGTATACCGCTACGCCATAGCCACTATATGCAATTGACAACATTGCACCAGAGTAGTTCCTAAGCGACTCTCCTTGATGATTCCACATAATGAGAAACTGATGTTTCATCACGTGGTGATAAATGCATTACCAGGATCTTCCTAGCGAATGTCCTTTCTGCATCTTTTTTGTTTATGATCCAGTGTCTAAGATGCGTGTATCTTAAACCTCCCTAGTATTTGCTTAACTGACTGAAGAACTGAAATTAGTGACACGAAGGGCATCGAACAAATAGTGATAGAAATGTTTCTTAAGGAATTGTTACTATGTTAGTAATCTTTTAGTTTCAATATTATGAATTGGTTTAATCAGTCAGACTTGGGCGCCTTTGCCAGAAAGGCCAAAGTTTTGTATGACATGGTGACAAGCTGATATCACTTACTGTTATTATGTTACTAGTTTTTTGTCCAAATGCGAACACTCATTGTGATGAATAAGATTGGATATATGAATATTACTGATGAATTATCGATTATCTTGATTACTTAATATGATTACTTGTCACTTTCAGGGTTGTGAGGTCTGCAGGACCTAGACAACACATCCTTAATCTTGGGCATGGTGTTCTTGTTGGCACACCTGAAGAAGCTGTTGCACATTTCTTTGATGTCGCGAGGAGCTTGAAGTTTGACACTCTCTCTCAAAATAACACTGCAAATGTAGCTTAA
Protein sequence

>MS.gene026855.t1

MMRQAGRYMAIYRKLAEKHPSFRERSETTDLIVEISLQPWKAFRPDGVIIFSDILTPLPAFGIDFDIEDVRGPVIQSPIRSEEGLKVLHPIDFDKLSFVGESLKILRQEVGGDAAVLGFVGAPWTLATYIVEGGTTRTYTTIKSMCHTAPHILRTLLSHLSKAIADYIVFQVESGAHCIQIFDSWGGQLPPHMWELWSKPYIKEIVNLVKKKCPDTPLVLYINGNGGLLERMIDTGVDVIGLDWTVDMADGRRRLGSGIGVQGNVDPASLFSPLTALTEEIQRVVRSAGPRQHILNLGHGVLVGTPEEAVAHFFDVARSLKFDTLSQNNTANVA