AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026899

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026899.t1 MTR_2g075300 89.810 422 39 2 15 435 61 479 0.0 793
MS.gene026899.t1 MTR_2g084535 60.599 434 152 4 15 430 63 495 0.0 556
MS.gene026899.t1 MTR_7g105400 44.270 445 214 7 16 433 61 498 4.36e-127 377
MS.gene026899.t1 MTR_7g105390 43.848 447 224 6 13 433 53 498 9.14e-125 371
MS.gene026899.t1 MTR_4g097590 40.421 475 233 9 6 435 54 523 1.08e-113 343
MS.gene026899.t1 MTR_7g105390 42.130 432 201 4 13 433 71 464 3.42e-110 333
MS.gene026899.t1 MTR_8g076210 30.310 419 226 18 15 400 21 406 2.49e-30 122
MS.gene026899.t1 MTR_5g021530 28.424 387 220 16 15 373 44 401 2.43e-23 102
MS.gene026899.t1 MTR_2g075270 86.047 43 6 0 393 435 45 87 5.85e-20 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026899.t1 AT5G22860 51.259 437 192 6 15 433 62 495 4.56e-153 443
MS.gene026899.t1 AT2G24280 45.815 454 216 6 7 434 42 491 3.88e-138 405
MS.gene026899.t1 AT5G22860 52.261 398 169 6 15 394 62 456 4.04e-137 401
MS.gene026899.t1 AT5G22860 51.706 381 163 6 15 377 62 439 2.43e-129 380
MS.gene026899.t1 AT5G22860 51.706 381 163 6 15 377 62 439 2.43e-129 380
MS.gene026899.t1 AT5G65760 43.046 453 228 6 5 432 56 503 7.29e-119 357
MS.gene026899.t1 AT2G24280 45.088 397 188 6 7 377 42 434 3.49e-115 344
MS.gene026899.t1 AT5G22860 50.282 354 155 6 15 350 62 412 5.94e-112 338
MS.gene026899.t1 AT5G22860 50.282 354 155 6 15 350 62 412 5.94e-112 338
MS.gene026899.t1 AT5G22860 50.282 354 155 6 15 350 62 412 5.94e-112 338
MS.gene026899.t1 AT5G22860 50.329 304 131 5 15 301 62 362 1.15e-93 287
MS.gene026899.t1 AT3G28680 45.087 173 90 3 133 300 18 190 1.10e-43 152
MS.gene026899.t1 AT4G36195 29.082 392 212 16 15 373 53 411 2.75e-24 105
MS.gene026899.t1 AT4G36195 29.082 392 212 16 15 373 53 411 3.71e-24 105
MS.gene026899.t1 AT4G36195 29.082 392 212 16 15 373 53 411 3.71e-24 105
MS.gene026899.t1 AT2G18080 26.980 404 228 15 15 384 53 423 1.14e-23 103
MS.gene026899.t1 AT4G36190 28.426 394 216 16 13 373 51 411 2.63e-23 102

Find 116 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCCCAATATTTGCTTATT+TGG 0.090901 2.2:+17996804 MS.gene026899:CDS
CCACCCAAATAAGCAAATAT+TGG 0.260148 2.2:-17996808 None:intergenic
GCCCCAATATTTGCTTATTT+GGG 0.276564 2.2:+17996805 MS.gene026899:CDS
CAGCGCTTGCTGCATGGTTT+CGG 0.295334 2.2:+17997743 MS.gene026899:intron
ACGAATCTATAAGAAATTCT+TGG 0.299771 2.2:+17998008 MS.gene026899:CDS
ATAGCCGTAAGATGTGTTAA+AGG 0.307501 2.2:-17997035 None:intergenic
CACCCAAATAAGCAAATATT+GGG 0.314162 2.2:-17996807 None:intergenic
GAACAGTGTCAGAAAGATTT+TGG 0.314945 2.2:+17998649 MS.gene026899:CDS
TTTGCACCAGTATATTTATC+TGG 0.315590 2.2:-17998391 None:intergenic
CTGATGGCAGGATATTAAAT+TGG 0.323382 2.2:+17998793 MS.gene026899:intron
CTTAGAAAGTCAAGACCATT+TGG 0.345243 2.2:-17998058 None:intergenic
TCTTGTTAGCACCGGGGTTA+CGG 0.352151 2.2:+17997001 MS.gene026899:intron
TGTTATTACAAGGGATTTCC+AGG 0.355365 2.2:+17997858 MS.gene026899:CDS
TACAAAATATCTCTGTTTGA+AGG 0.371699 2.2:-17996749 None:intergenic
CTTGTTAGCACCGGGGTTAC+GGG 0.379441 2.2:+17997002 MS.gene026899:intron
AGAAGTTCTGCTATATTTGA+AGG 0.387890 2.2:+17997090 MS.gene026899:CDS
GTTTACCCGCCACTACTATA+AGG 0.389378 2.2:-17998866 None:intergenic
CAGAGTTCTCAACAATATAT+CGG 0.390024 2.2:+17998985 MS.gene026899:intron
AACTCAACCGTGGAAGTGTC+AGG 0.394233 2.2:-17996600 None:intergenic
GTGTCCAACCTCGCCCTCAC+TGG 0.397994 2.2:+17998671 MS.gene026899:CDS
ATATATGCTGGTATGGTTGC+TGG 0.398273 2.2:+17998340 MS.gene026899:CDS
TGCCACCTCTTGCAGTCCCT+TGG 0.399043 2.2:+17998186 MS.gene026899:intron
GTTAATCCAGATAAATATAC+TGG 0.411412 2.2:+17998385 MS.gene026899:CDS
AGATTCGTAGCATGTCTTAC+TGG 0.414112 2.2:-17997993 None:intergenic
ATATTAAATTGGTTCTTAAG+AGG 0.428917 2.2:+17998804 MS.gene026899:CDS
GCAGTGGAGCTGATGAAGCC+AGG 0.436348 2.2:-17997795 None:intergenic
AGTCCCCGATGATTGTGGTT+GGG 0.445516 2.2:+17997131 MS.gene026899:CDS
ACATCATGGTAAGCACTTGC+TGG 0.449811 2.2:-17997838 None:intergenic
CCACGTTGCCATCGGCGCCC+TGG 0.451634 2.2:+17997777 MS.gene026899:CDS
GTCCCCGATGATTGTGGTTG+GGG 0.455350 2.2:+17997132 MS.gene026899:CDS
GCTGGATCACTTTCAGTTGA+AGG 0.458086 2.2:-17999292 None:intergenic
TTATGAGTTGAAAGACTACT+TGG 0.474066 2.2:+17998216 MS.gene026899:CDS
GGAGACATTGTTAATTCGCT+TGG 0.474625 2.2:+17996835 MS.gene026899:CDS
CTTTGTTGAACTAACCATGC+TGG 0.474625 2.2:-17999310 None:intergenic
AAGTAGTCTTTCAACTCATA+AGG 0.475658 2.2:-17998214 None:intergenic
TAGCCGTAAGATGTGTTAAA+GGG 0.480646 2.2:-17997034 None:intergenic
CACTTGACCTCCGTAGTAAG+AGG 0.482290 2.2:-17998698 None:intergenic
CACTGGATCACCTCTTACTA+CGG 0.483625 2.2:+17998688 MS.gene026899:CDS
CAGTAGGATATATGCTGGTA+TGG 0.483745 2.2:+17998333 MS.gene026899:CDS
GTGCAAAGCCATTTGATGCT+TGG 0.484214 2.2:+17998407 MS.gene026899:CDS
TGCCATCTCCAAGCATCAAA+TGG 0.486119 2.2:-17998415 None:intergenic
CACTACTATAAGGATCTCTT+AGG 0.486263 2.2:-17998856 None:intergenic
TTTGCTTATTTGGGTGGAGA+AGG 0.490409 2.2:+17996814 MS.gene026899:CDS
CTTACCATGATGTTATTACA+AGG 0.494198 2.2:+17997848 MS.gene026899:CDS
ATTATGCAGCGCTTGCTGCA+TGG 0.494968 2.2:+17997737 MS.gene026899:intron
TGATGAAGCCAGGGCGCCGA+TGG 0.496753 2.2:-17997785 None:intergenic
GCTGGTATGGTTGCTGGCTA+TGG 0.497013 2.2:+17998346 MS.gene026899:CDS
TGATCCAGTGAGGGCGAGGT+TGG 0.500209 2.2:-17998675 None:intergenic
CCAATATTTGCTTATTTGGG+TGG 0.501088 2.2:+17996808 MS.gene026899:CDS
CAGTGGAGCTGATGAAGCCA+GGG 0.504666 2.2:-17997794 None:intergenic
GTTAGTTCAACAAAGGAATA+CGG 0.509196 2.2:+17999318 MS.gene026899:CDS
CAGTTGAAGGTACAAGATCC+TGG 0.514821 2.2:-17999279 None:intergenic
AAGTGTCAGGGAGTTGGTGG+TGG 0.516165 2.2:-17996587 None:intergenic
GAAGTAGCATCTAAGCCAAA+TGG 0.516219 2.2:+17998043 MS.gene026899:CDS
CTTGGGGAACATGTATCAAA+AGG 0.521218 2.2:+17998234 MS.gene026899:CDS
AACTCATAAGGCTGTTCCAA+GGG 0.522619 2.2:-17998202 None:intergenic
GCCTCACAAATAGTCGTAAG+TGG 0.525609 2.2:-17998277 None:intergenic
GCCATTGATAGAGCTGCCTA+TGG 0.525815 2.2:+17998298 MS.gene026899:CDS
GTGCCCTTTAACACATCTTA+CGG 0.525900 2.2:+17997031 MS.gene026899:CDS
CTCGTGAAGTTGAAAGGTTG+AGG 0.534543 2.2:-17998619 None:intergenic
ATTTGATGCTTGGAGATGGC+AGG 0.535537 2.2:+17998417 MS.gene026899:CDS
CATACCATTGACTGTAGGTA+GGG 0.537541 2.2:-17999020 None:intergenic
AGCCATTTGATGCTTGGAGA+TGG 0.541350 2.2:+17998413 MS.gene026899:CDS
GCATATCTCGTGAAGTTGAA+AGG 0.541851 2.2:-17998625 None:intergenic
TGGAAGTGTCAGGGAGTTGG+TGG 0.541921 2.2:-17996590 None:intergenic
TCCATAGGCAGCTCTATCAA+TGG 0.545444 2.2:-17998299 None:intergenic
AATCCCTTGTAATAACATCA+TGG 0.547144 2.2:-17997852 None:intergenic
CAGGGCGCCGATGGCAACGT+GGG 0.551704 2.2:-17997776 None:intergenic
TATGAGTTGAAAGACTACTT+GGG 0.552868 2.2:+17998217 MS.gene026899:CDS
TAGTAGAGCTTTAAAAGAAG+TGG 0.553368 2.2:-17996884 None:intergenic
GTCCTCAGTAGGATATATGC+TGG 0.559053 2.2:+17998328 MS.gene026899:CDS
AAGTATCCCCACGTTGCCAT+CGG 0.561028 2.2:+17997769 MS.gene026899:CDS
AAGTCCCCGATGATTGTGGT+TGG 0.562729 2.2:+17997130 MS.gene026899:CDS
TTTCTGATAGATAGTTGAAG+TGG 0.566407 2.2:-17996717 None:intergenic
TGGTGGTAACAGAGAAACAT+AGG 0.572049 2.2:-17996570 None:intergenic
AGGTTGAGATAATCCAAAGC+TGG 0.575761 2.2:+17999341 MS.gene026899:CDS
GAGGTGATCCAGTGAGGGCG+AGG 0.575976 2.2:-17998679 None:intergenic
GAGATCCTTATAGTAGTGGC+GGG 0.576464 2.2:+17998861 MS.gene026899:CDS
ATAGTGATGCCTATCGGCAT+AGG 0.577307 2.2:+17998577 MS.gene026899:CDS
ACATACCATTGACTGTAGGT+AGG 0.577382 2.2:-17999021 None:intergenic
CCTCACAAATAGTCGTAAGT+GGG 0.579482 2.2:-17998276 None:intergenic
CCGTGGAAGTGTCAGGGAGT+TGG 0.584077 2.2:-17996593 None:intergenic
CTAAGAGATCCTTATAGTAG+TGG 0.586212 2.2:+17998857 MS.gene026899:CDS
GTTGCCCTACCTACAGTCAA+TGG 0.587397 2.2:+17999016 MS.gene026899:CDS
GAACTTCTGCGTAATCAGCA+AGG 0.588716 2.2:-17997075 None:intergenic
CCAGGGCGCCGATGGCAACG+TGG 0.590070 2.2:-17997777 None:intergenic
CAACTCATAAGGCTGTTCCA+AGG 0.594364 2.2:-17998203 None:intergenic
CAAGAATTTCCTATGCCGAT+AGG 0.596607 2.2:-17998586 None:intergenic
ACTGAGGACATCATCTCCAT+AGG 0.596855 2.2:-17998314 None:intergenic
AGAGATCCTTATAGTAGTGG+CGG 0.604183 2.2:+17998860 MS.gene026899:CDS
ACTGAAATAGTGATGCCTAT+CGG 0.607219 2.2:+17998571 MS.gene026899:CDS
AACTTCTGCGTAATCAGCAA+GGG 0.607600 2.2:-17997074 None:intergenic
CCAACTCCCTGACACTTCCA+CGG 0.616998 2.2:+17996593 MS.gene026899:CDS
CAGCATGGTTAGTTCAACAA+AGG 0.626050 2.2:+17999311 MS.gene026899:CDS
TTACCATGATGTTATTACAA+GGG 0.628091 2.2:+17997849 MS.gene026899:CDS
ATGTTATCAAAGTAGAGCAG+TGG 0.628222 2.2:-17997811 None:intergenic
ATGGAGATGATGTCCTCAGT+AGG 0.629079 2.2:+17998317 MS.gene026899:CDS
TCCCCGATGATTGTGGTTGG+GGG 0.629879 2.2:+17997133 MS.gene026899:CDS
CTCACAAATAGTCGTAAGTG+GGG 0.630633 2.2:-17998275 None:intergenic
GCACTGACTTCCCGTAACCC+CGG 0.631226 2.2:-17997012 None:intergenic
ACTCAACCGTGGAAGTGTCA+GGG 0.633050 2.2:-17996599 None:intergenic
CCCACTTACGACTATTTGTG+AGG 0.636619 2.2:+17998276 MS.gene026899:CDS
ATGAGTTGAAAGACTACTTG+GGG 0.641981 2.2:+17998218 MS.gene026899:CDS
ACAAAAGTCCCCGATGATTG+TGG 0.642669 2.2:+17997126 MS.gene026899:CDS
AGTAAGAGGTGATCCAGTGA+GGG 0.649906 2.2:-17998684 None:intergenic
AGGGCGCCGATGGCAACGTG+GGG 0.656511 2.2:-17997775 None:intergenic
CAACTGAAAGTGATCCAGCA+TGG 0.662989 2.2:+17999296 MS.gene026899:CDS
TAGTAAGAGGTGATCCAGTG+AGG 0.666324 2.2:-17998685 None:intergenic
ATATACATACCATTGACTGT+AGG 0.684175 2.2:-17999025 None:intergenic
AGCTCTACTAGTGTACATTG+AGG 0.685630 2.2:+17996897 MS.gene026899:CDS
ACCCAAATAAGCAAATATTG+GGG 0.688350 2.2:-17996806 None:intergenic
GGGGAACATGTATCAAAAGG+CGG 0.688405 2.2:+17998237 MS.gene026899:CDS
TGGATCACCTCTTACTACGG+AGG 0.695905 2.2:+17998691 MS.gene026899:CDS
TCACAAATAGTCGTAAGTGG+GGG 0.699413 2.2:-17998274 None:intergenic
AGTTCAACAAAGGAATACGG+AGG 0.703928 2.2:+17999321 MS.gene026899:CDS
TACCAGCATATATCCTACTG+AGG 0.704311 2.2:-17998330 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAATTTAAATATAAT+AGG - chr2.2:17997254-17997273 None:intergenic 0.0%
!!! TTTGATTTATCAAATATAAA+TGG + chr2.2:17997939-17997958 MS.gene026899:intron 10.0%
!! AAAAAAAAGAAGGAAATATA+TGG - chr2.2:17998118-17998137 None:intergenic 15.0%
!! AAGATCAAGTAAATAATTAT+TGG + chr2.2:17998469-17998488 MS.gene026899:intron 15.0%
!! ATTTATCAAATATAAATGGA+TGG + chr2.2:17997943-17997962 MS.gene026899:intron 15.0%
!! CAAACAAAAAAAAAAAAAGA+AGG - chr2.2:17998128-17998147 None:intergenic 15.0%
!!! ATTTTGTAAATTTCAAGTTT+TGG + chr2.2:17996764-17996783 MS.gene026899:CDS 15.0%
!!! TATAAAAGGTATGTTTTAAA+AGG - chr2.2:17997315-17997334 None:intergenic 15.0%
!!! TTTTGTAAATTTCAAGTTTT+GGG + chr2.2:17996765-17996784 MS.gene026899:CDS 15.0%
!!! TTTTTTTTTGTTTGAACAAT+AGG + chr2.2:17998134-17998153 MS.gene026899:intron 15.0%
!! AATTTAATTAATTGGTATGC+AGG + chr2.2:17997967-17997986 MS.gene026899:intron 20.0%
!! ACAATCTCTATTTAATTGAA+TGG + chr2.2:17997438-17997457 MS.gene026899:intron 20.0%
!! ATCAAGTAAATAATTATTGG+AGG + chr2.2:17998472-17998491 MS.gene026899:intron 20.0%
!! CAATCTCTATTTAATTGAAT+GGG + chr2.2:17997439-17997458 MS.gene026899:intron 20.0%
!! GGTATGCTTAAAATTATAAA+AGG - chr2.2:17997329-17997348 None:intergenic 20.0%
!! TATAGAACCATTTGATTAAT+TGG - chr2.2:17999223-17999242 None:intergenic 20.0%
!! TCTTAGTACATATACTAAAA+AGG + chr2.2:17997594-17997613 MS.gene026899:intron 20.0%
!! TGGATGGTAATTTAATTAAT+TGG + chr2.2:17997959-17997978 MS.gene026899:intron 20.0%
!! TTGTAAACCAATTAATCAAA+TGG + chr2.2:17999213-17999232 MS.gene026899:intron 20.0%
!!! AAACTATATGGTGTATTTTT+CGG + chr2.2:17997492-17997511 MS.gene026899:intron 20.0%
!!! AATATTTTTTTATCCAGCTT+TGG - chr2.2:17999357-17999376 None:intergenic 20.0%
!!! ATATTAAATTGGTTCTTAAG+AGG + chr2.2:17998804-17998823 MS.gene026899:CDS 20.0%
!!! GGAGGATTTTTTATATTATT+TGG + chr2.2:17998490-17998509 MS.gene026899:intron 20.0%
!!! TTTACAAATGTTGATTTTTC+AGG + chr2.2:17999247-17999266 MS.gene026899:intron 20.0%
!!! TTTGTAAATTTCAAGTTTTG+GGG + chr2.2:17996766-17996785 MS.gene026899:CDS 20.0%
! ACGAATCTATAAGAAATTCT+TGG + chr2.2:17998008-17998027 MS.gene026899:CDS 25.0%
! CAGATCTTCATTTAAATTGA+AGG - chr2.2:17996668-17996687 None:intergenic 25.0%
! CTGAAATATATAAAAGCCAA+AGG - chr2.2:17998968-17998987 None:intergenic 25.0%
! GTTAATCCAGATAAATATAC+TGG + chr2.2:17998385-17998404 MS.gene026899:CDS 25.0%
! TACAAAATATCTCTGTTTGA+AGG - chr2.2:17996752-17996771 None:intergenic 25.0%
! TATAAAGCAGAAACTTTCTT+TGG - chr2.2:17997350-17997369 None:intergenic 25.0%
! TTACCATGATGTTATTACAA+GGG + chr2.2:17997849-17997868 MS.gene026899:CDS 25.0%
! TTGAAAAAACAGAAAAACGA+TGG - chr2.2:17997216-17997235 None:intergenic 25.0%
!! AAAAAAATATAGTTTGCGAC+AGG - chr2.2:17997564-17997583 None:intergenic 25.0%
!! AAAAAATATAGTTTGCGACA+GGG - chr2.2:17997563-17997582 None:intergenic 25.0%
!!! AATTGAAGGTTTTAGTGTTT+TGG - chr2.2:17996654-17996673 None:intergenic 25.0%
!!! ATACACCATATAGTTTTACT+TGG - chr2.2:17997488-17997507 None:intergenic 25.0%
!!! ATGATTATGTTTTTCTAGAG+TGG - chr2.2:17997658-17997677 None:intergenic 25.0%
!!! GTATGTTTTAAAAGGTTTCA+AGG - chr2.2:17997307-17997326 None:intergenic 25.0%
!!! GTTTTAAAATAAGCTTCCTT+TGG + chr2.2:17998949-17998968 MS.gene026899:intron 25.0%
!!! TTTAATTTTCTTGTTAGCAC+CGG + chr2.2:17996993-17997012 MS.gene026899:intron 25.0%
AAGTAGTCTTTCAACTCATA+AGG - chr2.2:17998217-17998236 None:intergenic 30.0%
AATCCCTTGTAATAACATCA+TGG - chr2.2:17997855-17997874 None:intergenic 30.0%
ACCCAAATAAGCAAATATTG+GGG - chr2.2:17996809-17996828 None:intergenic 30.0%
AGAAGTTCTGCTATATTTGA+AGG + chr2.2:17997090-17997109 MS.gene026899:CDS 30.0%
CACCCAAATAAGCAAATATT+GGG - chr2.2:17996810-17996829 None:intergenic 30.0%
CAGAGTTCTCAACAATATAT+CGG + chr2.2:17998985-17999004 MS.gene026899:intron 30.0%
CTTACCATGATGTTATTACA+AGG + chr2.2:17997848-17997867 MS.gene026899:CDS 30.0%
GTTAGTTCAACAAAGGAATA+CGG + chr2.2:17999318-17999337 MS.gene026899:CDS 30.0%
TGCGTACTTCTATTATGTTT+AGG + chr2.2:17998719-17998738 MS.gene026899:intron 30.0%
TTTCTGATAGATAGTTGAAG+TGG - chr2.2:17996720-17996739 None:intergenic 30.0%
TTTGCACCAGTATATTTATC+TGG - chr2.2:17998394-17998413 None:intergenic 30.0%
! AGCAACATCATTTTCTCAAA+TGG + chr2.2:17998833-17998852 MS.gene026899:CDS 30.0%
! ATATACATACCATTGACTGT+AGG - chr2.2:17999028-17999047 None:intergenic 30.0%
! TATGAGTTGAAAGACTACTT+GGG + chr2.2:17998217-17998236 MS.gene026899:CDS 30.0%
! TTAATTTTCTTGTTAGCACC+GGG + chr2.2:17996994-17997013 MS.gene026899:intron 30.0%
! TTATGAGTTGAAAGACTACT+TGG + chr2.2:17998216-17998235 MS.gene026899:CDS 30.0%
!! AATAACTATGCTGATTTACC+TGG - chr2.2:17997879-17997898 None:intergenic 30.0%
!! TAGTAGAGCTTTAAAAGAAG+TGG - chr2.2:17996887-17996906 None:intergenic 30.0%
!! TCAAGGAGTATTGTAATTAC+AGG - chr2.2:17997290-17997309 None:intergenic 30.0%
!!! GTTAATTCGCTTGGTTTTAT+CGG + chr2.2:17996844-17996863 MS.gene026899:CDS 30.0%
!!! TGGTTTTATCGGTTTTATGA+CGG + chr2.2:17996855-17996874 MS.gene026899:CDS 30.0%
ACTGAAATAGTGATGCCTAT+CGG + chr2.2:17998571-17998590 MS.gene026899:CDS 35.0%
ATAGCCGTAAGATGTGTTAA+AGG - chr2.2:17997038-17997057 None:intergenic 35.0%
ATGTTATCAAAGTAGAGCAG+TGG - chr2.2:17997814-17997833 None:intergenic 35.0%
CACTACTATAAGGATCTCTT+AGG - chr2.2:17998859-17998878 None:intergenic 35.0%
CCAATATTTGCTTATTTGGG+TGG + chr2.2:17996808-17996827 MS.gene026899:CDS 35.0%
CCACCCAAATAAGCAAATAT+TGG - chr2.2:17996811-17996830 None:intergenic 35.0%
CTAAGAGATCCTTATAGTAG+TGG + chr2.2:17998857-17998876 MS.gene026899:CDS 35.0%
CTGATGGCAGGATATTAAAT+TGG + chr2.2:17998793-17998812 MS.gene026899:intron 35.0%
CTTAGAAAGTCAAGACCATT+TGG - chr2.2:17998061-17998080 None:intergenic 35.0%
GCCCCAATATTTGCTTATTT+GGG + chr2.2:17996805-17996824 MS.gene026899:CDS 35.0%
GCTATCATCAATGTGATGAT+TGG - chr2.2:17997534-17997553 None:intergenic 35.0%
GTGCACCAAGTAAAACTATA+TGG + chr2.2:17997480-17997499 MS.gene026899:intron 35.0%
TAGCCGTAAGATGTGTTAAA+GGG - chr2.2:17997037-17997056 None:intergenic 35.0%
TGATGATTGGCAGATTATTG+AGG - chr2.2:17997521-17997540 None:intergenic 35.0%
TGCCCCAATATTTGCTTATT+TGG + chr2.2:17996804-17996823 MS.gene026899:CDS 35.0%
TGTTATTACAAGGGATTTCC+AGG + chr2.2:17997858-17997877 MS.gene026899:CDS 35.0%
! ATGAGTTGAAAGACTACTTG+GGG + chr2.2:17998218-17998237 MS.gene026899:CDS 35.0%
! GAACAGTGTCAGAAAGATTT+TGG + chr2.2:17998649-17998668 MS.gene026899:CDS 35.0%
! GGATATCTTTTGTCAAGACT+TGG + chr2.2:17997615-17997634 MS.gene026899:intron 35.0%
! GGTTGAGTTCATTTTCAGAA+TGG + chr2.2:17996614-17996633 MS.gene026899:intron 35.0%
! TAATTTTCTTGTTAGCACCG+GGG + chr2.2:17996995-17997014 MS.gene026899:intron 35.0%
!!! GTAAATTTCAAGTTTTGGGG+TGG + chr2.2:17996769-17996788 MS.gene026899:CDS 35.0%
AACTCATAAGGCTGTTCCAA+GGG - chr2.2:17998205-17998224 None:intergenic 40.0%
AACTTCTGCGTAATCAGCAA+GGG - chr2.2:17997077-17997096 None:intergenic 40.0%
AATTGCATCGTTGTGACTGA+TGG + chr2.2:17998777-17998796 MS.gene026899:intron 40.0%
AGAGATCCTTATAGTAGTGG+CGG + chr2.2:17998860-17998879 MS.gene026899:CDS 40.0%
AGATTCGTAGCATGTCTTAC+TGG - chr2.2:17997996-17998015 None:intergenic 40.0%
AGCTCTACTAGTGTACATTG+AGG + chr2.2:17996897-17996916 MS.gene026899:CDS 40.0%
AGGTTGAGATAATCCAAAGC+TGG + chr2.2:17999341-17999360 MS.gene026899:CDS 40.0%
AGTTCAACAAAGGAATACGG+AGG + chr2.2:17999321-17999340 MS.gene026899:CDS 40.0%
ATATATGCTGGTATGGTTGC+TGG + chr2.2:17998340-17998359 MS.gene026899:CDS 40.0%
CAAGAATTTCCTATGCCGAT+AGG - chr2.2:17998589-17998608 None:intergenic 40.0%
CAGTAGGATATATGCTGGTA+TGG + chr2.2:17998333-17998352 MS.gene026899:CDS 40.0%
CCTCACAAATAGTCGTAAGT+GGG - chr2.2:17998279-17998298 None:intergenic 40.0%
CTCACAAATAGTCGTAAGTG+GGG - chr2.2:17998278-17998297 None:intergenic 40.0%
CTTGGGGAACATGTATCAAA+AGG + chr2.2:17998234-17998253 MS.gene026899:CDS 40.0%
CTTTGTTGAACTAACCATGC+TGG - chr2.2:17999313-17999332 None:intergenic 40.0%
GAAGTAGCATCTAAGCCAAA+TGG + chr2.2:17998043-17998062 MS.gene026899:CDS 40.0%
GCATATCTCGTGAAGTTGAA+AGG - chr2.2:17998628-17998647 None:intergenic 40.0%
GGAGACATTGTTAATTCGCT+TGG + chr2.2:17996835-17996854 MS.gene026899:CDS 40.0%
GTGCCCTTTAACACATCTTA+CGG + chr2.2:17997031-17997050 MS.gene026899:CDS 40.0%
TACCAGCATATATCCTACTG+AGG - chr2.2:17998333-17998352 None:intergenic 40.0%
TCACAAATAGTCGTAAGTGG+GGG - chr2.2:17998277-17998296 None:intergenic 40.0%
TCTGAAAATGAACTCAACCG+TGG - chr2.2:17996613-17996632 None:intergenic 40.0%
TTTGCTTATTTGGGTGGAGA+AGG + chr2.2:17996814-17996833 MS.gene026899:CDS 40.0%
! ACATACCATTGACTGTAGGT+AGG - chr2.2:17999024-17999043 None:intergenic 40.0%
! CAGCATGGTTAGTTCAACAA+AGG + chr2.2:17999311-17999330 MS.gene026899:CDS 40.0%
! CATACCATTGACTGTAGGTA+GGG - chr2.2:17999023-17999042 None:intergenic 40.0%
!!! TTTTTCAGGAACTCATTGCC+AGG + chr2.2:17999261-17999280 MS.gene026899:intron 40.0%
ACAAAAGTCCCCGATGATTG+TGG + chr2.2:17997126-17997145 MS.gene026899:CDS 45.0%
ACATCATGGTAAGCACTTGC+TGG - chr2.2:17997841-17997860 None:intergenic 45.0%
ACTGAGGACATCATCTCCAT+AGG - chr2.2:17998317-17998336 None:intergenic 45.0%
AGCCATTTGATGCTTGGAGA+TGG + chr2.2:17998413-17998432 MS.gene026899:CDS 45.0%
AGTAAGAGGTGATCCAGTGA+GGG - chr2.2:17998687-17998706 None:intergenic 45.0%
ATAGTGATGCCTATCGGCAT+AGG + chr2.2:17998577-17998596 MS.gene026899:CDS 45.0%
ATGGAGATGATGTCCTCAGT+AGG + chr2.2:17998317-17998336 MS.gene026899:CDS 45.0%
ATTTGATGCTTGGAGATGGC+AGG + chr2.2:17998417-17998436 MS.gene026899:CDS 45.0%
CAACTCATAAGGCTGTTCCA+AGG - chr2.2:17998206-17998225 None:intergenic 45.0%
CAACTGAAAGTGATCCAGCA+TGG + chr2.2:17999296-17999315 MS.gene026899:CDS 45.0%
CACTGGATCACCTCTTACTA+CGG + chr2.2:17998688-17998707 MS.gene026899:CDS 45.0%
CCCACTTACGACTATTTGTG+AGG + chr2.2:17998276-17998295 MS.gene026899:CDS 45.0%
CTCGTGAAGTTGAAAGGTTG+AGG - chr2.2:17998622-17998641 None:intergenic 45.0%
GAACTTCTGCGTAATCAGCA+AGG - chr2.2:17997078-17997097 None:intergenic 45.0%
GAGATCCTTATAGTAGTGGC+GGG + chr2.2:17998861-17998880 MS.gene026899:CDS 45.0%
GCCTCACAAATAGTCGTAAG+TGG - chr2.2:17998280-17998299 None:intergenic 45.0%
GCTGGATCACTTTCAGTTGA+AGG - chr2.2:17999295-17999314 None:intergenic 45.0%
GGGGAACATGTATCAAAAGG+CGG + chr2.2:17998237-17998256 MS.gene026899:CDS 45.0%
GTCCTCAGTAGGATATATGC+TGG + chr2.2:17998328-17998347 MS.gene026899:CDS 45.0%
GTGCAAAGCCATTTGATGCT+TGG + chr2.2:17998407-17998426 MS.gene026899:CDS 45.0%
GTTTACCCGCCACTACTATA+AGG - chr2.2:17998869-17998888 None:intergenic 45.0%
TAGTAAGAGGTGATCCAGTG+AGG - chr2.2:17998688-17998707 None:intergenic 45.0%
TCCATAGGCAGCTCTATCAA+TGG - chr2.2:17998302-17998321 None:intergenic 45.0%
TGCCATCTCCAAGCATCAAA+TGG - chr2.2:17998418-17998437 None:intergenic 45.0%
! CAGTTGAAGGTACAAGATCC+TGG - chr2.2:17999282-17999301 None:intergenic 45.0%
AAGTATCCCCACGTTGCCAT+CGG + chr2.2:17997769-17997788 MS.gene026899:CDS 50.0%
AAGTCCCCGATGATTGTGGT+TGG + chr2.2:17997130-17997149 MS.gene026899:CDS 50.0%
AGTCCCCGATGATTGTGGTT+GGG + chr2.2:17997131-17997150 MS.gene026899:CDS 50.0%
ATCCCCCAACCACAATCATC+GGG - chr2.2:17997138-17997157 None:intergenic 50.0%
CACTTGACCTCCGTAGTAAG+AGG - chr2.2:17998701-17998720 None:intergenic 50.0%
GATCCCCCAACCACAATCAT+CGG - chr2.2:17997139-17997158 None:intergenic 50.0%
GCCATTGATAGAGCTGCCTA+TGG + chr2.2:17998298-17998317 MS.gene026899:CDS 50.0%
GTTGCCCTACCTACAGTCAA+TGG + chr2.2:17999016-17999035 MS.gene026899:CDS 50.0%
TGGATCACCTCTTACTACGG+AGG + chr2.2:17998691-17998710 MS.gene026899:CDS 50.0%
! ATTATGCAGCGCTTGCTGCA+TGG + chr2.2:17997737-17997756 MS.gene026899:intron 50.0%
! TCTTGTTAGCACCGGGGTTA+CGG + chr2.2:17997001-17997020 MS.gene026899:intron 50.0%
!! AACTCAACCGTGGAAGTGTC+AGG - chr2.2:17996603-17996622 None:intergenic 50.0%
!! ACTCAACCGTGGAAGTGTCA+GGG - chr2.2:17996602-17996621 None:intergenic 50.0%
CAGTGGAGCTGATGAAGCCA+GGG - chr2.2:17997797-17997816 None:intergenic 55.0%
CCAACTCCCTGACACTTCCA+CGG + chr2.2:17996593-17996612 MS.gene026899:CDS 55.0%
CTGTTCCAAGGGACTGCAAG+AGG - chr2.2:17998194-17998213 None:intergenic 55.0%
GCATCGTTGTGACTGATGGC+AGG + chr2.2:17998781-17998800 MS.gene026899:intron 55.0%
GTCCCCGATGATTGTGGTTG+GGG + chr2.2:17997132-17997151 MS.gene026899:CDS 55.0%
GTGGTTGGGGGATCATATGC+TGG + chr2.2:17997145-17997164 MS.gene026899:CDS 55.0%
TCCCCCAACCACAATCATCG+GGG - chr2.2:17997137-17997156 None:intergenic 55.0%
TCCCCGATGATTGTGGTTGG+GGG + chr2.2:17997133-17997152 MS.gene026899:CDS 55.0%
TTCCAAGGGACTGCAAGAGG+TGG - chr2.2:17998191-17998210 None:intergenic 55.0%
! CAGCGCTTGCTGCATGGTTT+CGG + chr2.2:17997743-17997762 MS.gene026899:intron 55.0%
! CTTGTTAGCACCGGGGTTAC+GGG + chr2.2:17997002-17997021 MS.gene026899:intron 55.0%
! GCTGGTATGGTTGCTGGCTA+TGG + chr2.2:17998346-17998365 MS.gene026899:CDS 55.0%
!! AAGTGTCAGGGAGTTGGTGG+TGG - chr2.2:17996590-17996609 None:intergenic 55.0%
!! TGGAAGTGTCAGGGAGTTGG+TGG - chr2.2:17996593-17996612 None:intergenic 55.0%
GCAGTGGAGCTGATGAAGCC+AGG - chr2.2:17997798-17997817 None:intergenic 60.0%
TGATCCAGTGAGGGCGAGGT+TGG - chr2.2:17998678-17998697 None:intergenic 60.0%
TGCCACCTCTTGCAGTCCCT+TGG + chr2.2:17998186-17998205 MS.gene026899:intron 60.0%
!! CCGTGGAAGTGTCAGGGAGT+TGG - chr2.2:17996596-17996615 None:intergenic 60.0%
!! GCACTGACTTCCCGTAACCC+CGG - chr2.2:17997015-17997034 None:intergenic 60.0%
GAGGTGATCCAGTGAGGGCG+AGG - chr2.2:17998682-17998701 None:intergenic 65.0%
GTGTCCAACCTCGCCCTCAC+TGG + chr2.2:17998671-17998690 MS.gene026899:CDS 65.0%
!! TGATGAAGCCAGGGCGCCGA+TGG - chr2.2:17997788-17997807 None:intergenic 65.0%
!! AGGGCGCCGATGGCAACGTG+GGG - chr2.2:17997778-17997797 None:intergenic 70.0%
!! CAGGGCGCCGATGGCAACGT+GGG - chr2.2:17997779-17997798 None:intergenic 70.0%
CCACGTTGCCATCGGCGCCC+TGG + chr2.2:17997777-17997796 MS.gene026899:CDS 75.0%
!! CCAGGGCGCCGATGGCAACG+TGG - chr2.2:17997780-17997799 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 17996573 17999396 17996573 ID=MS.gene026899
chr2.2 mRNA 17996573 17999396 17996573 ID=MS.gene026899.t1;Parent=MS.gene026899
chr2.2 exon 17996573 17996614 17996573 ID=MS.gene026899.t1.exon1;Parent=MS.gene026899.t1
chr2.2 CDS 17996573 17996614 17996573 ID=cds.MS.gene026899.t1;Parent=MS.gene026899.t1
chr2.2 exon 17996709 17996918 17996709 ID=MS.gene026899.t1.exon2;Parent=MS.gene026899.t1
chr2.2 CDS 17996709 17996918 17996709 ID=cds.MS.gene026899.t1;Parent=MS.gene026899.t1
chr2.2 exon 17997010 17997169 17997010 ID=MS.gene026899.t1.exon3;Parent=MS.gene026899.t1
chr2.2 CDS 17997010 17997169 17997010 ID=cds.MS.gene026899.t1;Parent=MS.gene026899.t1
chr2.2 exon 17997746 17997879 17997746 ID=MS.gene026899.t1.exon4;Parent=MS.gene026899.t1
chr2.2 CDS 17997746 17997879 17997746 ID=cds.MS.gene026899.t1;Parent=MS.gene026899.t1
chr2.2 exon 17997989 17998101 17997989 ID=MS.gene026899.t1.exon5;Parent=MS.gene026899.t1
chr2.2 CDS 17997989 17998101 17997989 ID=cds.MS.gene026899.t1;Parent=MS.gene026899.t1
chr2.2 exon 17998201 17998438 17998201 ID=MS.gene026899.t1.exon6;Parent=MS.gene026899.t1
chr2.2 CDS 17998201 17998438 17998201 ID=cds.MS.gene026899.t1;Parent=MS.gene026899.t1
chr2.2 exon 17998565 17998717 17998565 ID=MS.gene026899.t1.exon7;Parent=MS.gene026899.t1
chr2.2 CDS 17998565 17998717 17998565 ID=cds.MS.gene026899.t1;Parent=MS.gene026899.t1
chr2.2 exon 17998803 17998882 17998803 ID=MS.gene026899.t1.exon8;Parent=MS.gene026899.t1
chr2.2 CDS 17998803 17998882 17998803 ID=cds.MS.gene026899.t1;Parent=MS.gene026899.t1
chr2.2 exon 17998988 17999037 17998988 ID=MS.gene026899.t1.exon9;Parent=MS.gene026899.t1
chr2.2 CDS 17998988 17999037 17998988 ID=cds.MS.gene026899.t1;Parent=MS.gene026899.t1
chr2.2 exon 17999269 17999396 17999269 ID=MS.gene026899.t1.exon10;Parent=MS.gene026899.t1
chr2.2 CDS 17999269 17999396 17999269 ID=cds.MS.gene026899.t1;Parent=MS.gene026899.t1
Gene Sequence

>MS.gene026899

ATGTTTCTCTGTTACCACCACCAACTCCCTGACACTTCCACGGTTGAGTTCATTTTCAGAATGGAAAACTACTAAGTACCAAAACACTAAAACCTTCAATTTAAATGAAGATCTGCATACATATTTCTATGAACAGACTTTAGACCACTTCAACTATCTATCAGAAAGCTACAAAACCTTCAAACAGAGATATTTTGTAAATTTCAAGTTTTGGGGTGGTGCTAATTCTAGTGCCCCAATATTTGCTTATTTGGGTGGAGAAGGAGACATTGTTAATTCGCTTGGTTTTATCGGTTTTATGACGGATAATGCCACTTCTTTTAAAGCTCTACTAGTGTACATTGAGGTACTAAATAGAAAACTATTTCTTTTGACACTCATTGATCTAAATCAAATTAGTTTTACGATTCTGATAAAACTTTTAATTTTCTTGTTAGCACCGGGGTTACGGGAAGTCAGTGCCCTTTAACACATCTTACGGCTATTTAAACTCAGCACAAGCCCTTGCTGATTACGCAGAAGTTCTGCTATATTTGAAGGATGCACTACATGCACAAAAGTCCCCGATGATTGTGGTTGGGGGATCATATGCTGGAAGTTAGTTTTTTAAATTATGTTTATGTTTTTCTTCACATTTTTACCATCGTTTTTCTGTTTTTTCAATTTTTTCTACTTAATCCTATTATATTTAAATTATATTTTTAAAATTTGAAACCTGTAATTACAATACTCCTTGAAACCTTTTAAAACATACCTTTTATAATTTTAAGCATACCAAAGAAAGTTTCTGCTTTATATAAAAGTTTCAGAATATTAGTTGCATATGTATGAAGTGTTAAAATCAATTATTTCTAAGCTTAAACAAACAATCTCTATTTAATTGAATGGGAAAATTTCTCATTGTAAAGTGCACCAAGTAAAACTATATGGTGTATTTTTCGGTGACCTCAATAATCTGCCAATCATCACATTGATGATAGCTGAAATCCCTGTCGCAAACTATATTTTTTTTCAAGATTACTCTTAGTACATATACTAAAAAGGATATCTTTTGTCAAGACTTGGTAAGTATTAGTACTATACCACTCTAGAAAAACATAATCATACAAAAACAAAGTCTTATTGTGTTGATGTGATCTTAATTAATGTCACATGTATTTGTTAATTATGCAGCGCTTGCTGCATGGTTTCGGCTTAAGTATCCCCACGTTGCCATCGGCGCCCTGGCTTCATCAGCTCCACTGCTCTACTTTGATAACATCACACCAGCAAGTGCTTACCATGATGTTATTACAAGGGATTTCCAGGTAAATCAGCATAGTTATTTTTTTTATCTTTTATGTTTGTTGATTAATATATATTTATTTTTGATTTATCAAATATAAATGGATGGTAATTTAATTAATTGGTATGCAGGAAACCAGTAAGACATGCTACGAATCTATAAGAAATTCTTGGTTTGAAATTGATGAAGTAGCATCTAAGCCAAATGGTCTTGACTTTCTAAGCAAGAAATTCAACACTTGCTAGTAAACACTGTTTCCATATATTTCCTTCTTTTTTTTTTTTTGTTTGAACAATAGGATCAAGTTCTGACTTAAACTTGTTCAAATTGCCACCTCTTGCAGTCCCTTGGAACAGCCTTATGAGTTGAAAGACTACTTGGGGAACATGTATCAAAAGGCGGCACAGTACAATGATCCCCCACTTACGACTATTTGTGAGGCCATTGATAGAGCTGCCTATGGAGATGATGTCCTCAGTAGGATATATGCTGGTATGGTTGCTGGCTATGGCAACAAAAAATGCAATGTTAATCCAGATAAATATACTGGTGCAAAGCCATTTGATGCTTGGAGATGGCAGGTAGTCACATTGAAATTTGAGTAATTATATAAGATCAAGTAAATAATTATTGGAGGATTTTTTATATTATTTGGTGCACATATTTCGTTGTTCTAAAAAAAATTATGAATTATTTTATGCATTCAGACGTGCACTGAAATAGTGATGCCTATCGGCATAGGAAATTCTTGCTTGCTTCAACCTCAACCTTTCAACTTCACGAGATATGCTGAACAGTGTCAGAAAGATTTTGGTGTCCAACCTCGCCCTCACTGGATCACCTCTTACTACGGAGGTCAAGTGCGTACTTCTATTATGTTTAGGTTTCAATATTAATATTACATTATCAATAGAAAATCAATTGCATCGTTGTGACTGATGGCAGGATATTAAATTGGTTCTTAAGAGGTTTGCGAGCAACATCATTTTCTCAAATGGCCTAAGAGATCCTTATAGTAGTGGCGGGTAAACATGCAAAATCTAAAAAATAAATTCATCTTAATTATTTTAGAATAATTATTTTAGAACAATGTTTTAAAATAAGCTTCCTTTGGCTTTTATATATTTCAGAGTTCTCAACAATATATCGGATAGTCTTGTTGCCCTACCTACAGTCAATGGTATGTATATCTTACTTTTTTTTTTTGAAATTATGTATGTCTTGTATTGTGTGAATACATACAATTTCGTTGTCTAATTATATTATATTATGCACATTCAGATGTAAGTATATAGAGAATACATTGTAGTAGTAGTAAGAGTACGTTGCTAACTTTTTATCACTTTTATAAAAAATTGTAAACCAATTAATCAAATGGTTCTATAATGTTTTACAAATGTTGATTTTTCAGGAACTCATTGCCAGGATCTTGTACCTTCAACTGAAAGTGATCCAGCATGGTTAGTTCAACAAAGGAATACGGAGGTTGAGATAATCCAAAGCTGGATAAAAAAATATTATGATGAAGTTAATATATAA
Protein sequence

>MS.gene026899.t1

MFLCYHHQLPDTSTTLDHFNYLSESYKTFKQRYFVNFKFWGGANSSAPIFAYLGGEGDIVNSLGFIGFMTDNATSFKALLVYIEHRGYGKSVPFNTSYGYLNSAQALADYAEVLLYLKDALHAQKSPMIVVGGSYAGTLAAWFRLKYPHVAIGALASSAPLLYFDNITPASAYHDVITRDFQETSKTCYESIRNSWFEIDEVASKPNGLDFLSKKFNTCYPLEQPYELKDYLGNMYQKAAQYNDPPLTTICEAIDRAAYGDDVLSRIYAGMVAGYGNKKCNVNPDKYTGAKPFDAWRWQTCTEIVMPIGIGNSCLLQPQPFNFTRYAEQCQKDFGVQPRPHWITSYYGGQDIKLVLKRFASNIIFSNGLRDPYSSGGVLNNISDSLVALPTVNGTHCQDLVPSTESDPAWLVQQRNTEVEIIQSWIKKYYDEVNI