AlfalfaGEDB | MS.gene027319

M. sativa cultivar XinJiangDaYe / MS.gene027319

KEGG: K02935
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00542.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027319.t1	PNX93291.1	89.4	217	23	0	1	217	1	217	9.70E-98	366.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027319.t1	P53163	36.5	167	96	4	61	217	28	194	2.1e-16	87.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027319.t1	A0A2K3MR37	89.4	217	23	0	1	217	1	217	7.0e-98	366.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene027319	MS.gene09988	PPI
MS.gene027319	MS.gene005792	PPI
MS.gene027319	MS.gene68085	PPI
MS.gene027319	MS.gene60517	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027319.t1	MTR_7g053520	96.313	217	8	0	1	217	1	217	5.80e-144	400
MS.gene027319.t1	MTR_2g064300	86.175	217	29	1	1	217	1	216	9.82e-124	349
MS.gene027319.t1	MTR_2g031760	41.212	165	92	3	54	217	30	190	2.40e-35	124
MS.gene027319.t1	MTR_8g076780	47.973	148	67	2	79	217	49	195	1.03e-31	114
MS.gene027319.t1	MTR_2g031960	48.872	133	63	2	86	217	27	155	2.44e-30	110

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027319.t1	AT1G70190	56.561	221	79	4	1	217	1	208	2.78e-68	208
MS.gene027319.t1	AT1G70190	56.561	221	79	4	1	217	1	208	2.78e-68	208
MS.gene027319.t1	AT4G36420	48.684	152	73	3	70	217	29	179	5.83e-34	120
MS.gene027319.t1	AT3G06040	45.333	150	73	3	69	217	45	186	4.47e-32	115
MS.gene027319.t1	AT3G06040	45.333	150	73	3	69	217	45	186	4.47e-32	115
MS.gene027319.t1	AT3G06040	45.333	150	73	3	69	217	45	186	4.47e-32	115
MS.gene027319.t1	AT4G37660	70.588	68	20	0	150	217	100	167	1.86e-25	97.8
MS.gene027319.t1	AT2G03130	59.494	79	32	0	139	217	13	91	7.56e-16	71.2

Find 48 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTGATGACCTTAATTT+TGG	0.127344	7.1:-48635458	None:intergenic
GAAACCAGGATCTGTAAATT+TGG	0.208162	7.1:+48635339	MS.gene027319:CDS
ACACCTTTCTTTATAATAGA+AGG	0.301571	7.1:-48635538	None:intergenic
TGAAGCAGCTTCCAAAATTA+AGG	0.341436	7.1:+48635447	MS.gene027319:CDS
TCTAGCTGAAGGTGCAGAAT+TGG	0.346308	7.1:+48635261	MS.gene027319:CDS
TTGTCTATACAACAAACTAT+TGG	0.364579	7.1:-48635011	None:intergenic
CCTCTTACTGCTAGCACTCT+AGG	0.374115	7.1:+48635034	MS.gene027319:CDS
CCAGCCAAATTTACAGATCC+TGG	0.386608	7.1:-48635343	None:intergenic
GAAGGAGGCAAAGGATTTAG+TGG	0.417133	7.1:+48635507	MS.gene027319:CDS
TGTCTATACAACAAACTATT+GGG	0.417171	7.1:-48635010	None:intergenic
CTTTCCTTGCAGCTTGACCT+AGG	0.439350	7.1:-48635093	None:intergenic
CCAGGATCTGTAAATTTGGC+TGG	0.453905	7.1:+48635343	MS.gene027319:CDS
CTAGCTGAAGGTGCAGAATT+GGG	0.458679	7.1:+48635262	MS.gene027319:CDS
CCTAGAGTGCTAGCAGTAAG+AGG	0.460957	7.1:-48635034	None:intergenic
ATCTGTAAATTTGGCTGGAA+TGG	0.461697	7.1:+48635348	MS.gene027319:CDS
TCTAGGCGCAATTGTCGTTA+TGG	0.475224	7.1:+48635603	MS.gene027319:CDS
TCAGTAGGATCAAATGTTGC+AGG	0.479265	7.1:-48635178	None:intergenic
AAAGGTGTTTCGAAGGAAGA+AGG	0.479617	7.1:+48635553	MS.gene027319:CDS
GGTTTCATAAATCCCACATT+GGG	0.482477	7.1:-48635322	None:intergenic
ACACCTTCTATTATAAAGAA+AGG	0.509780	7.1:+48635535	MS.gene027319:CDS
GTTGCAGGATCAAAATCAGT+TGG	0.513514	7.1:-48635163	None:intergenic
AGCTGCAAGGAAAGAGGAGG+AGG	0.515901	7.1:+48635102	MS.gene027319:CDS
AATGTATTTACAAGAAGCCT+AGG	0.520574	7.1:+48635076	MS.gene027319:CDS
CTGAAAACTCTTTCCGATGG+AGG	0.526100	7.1:-48635211	None:intergenic
TTCCAAAATTAAGGTCATCA+AGG	0.526956	7.1:+48635456	MS.gene027319:CDS
TTTCAGGCTTGTTGATGAAG+TGG	0.533852	7.1:+48635228	MS.gene027319:CDS
TATAAAGAAAGGTGTTTCGA+AGG	0.534435	7.1:+48635546	MS.gene027319:CDS
AAGGTCATCAAGGAGGTTCG+AGG	0.535857	7.1:+48635466	MS.gene027319:CDS
TGATCATCGTGGGCCTCCAT+CGG	0.539706	7.1:+48635198	MS.gene027319:CDS
CTCTTACTGCTAGCACTCTA+GGG	0.540669	7.1:+48635035	MS.gene027319:CDS
ATAGAGAAATTGAAAGCTCT+AGG	0.542277	7.1:+48635586	MS.gene027319:CDS
TTTAGGTTTGAAGGAGGCAA+AGG	0.553743	7.1:+48635498	MS.gene027319:CDS
AGGTCAAGCTGCAAGGAAAG+AGG	0.555154	7.1:+48635096	MS.gene027319:CDS
TTGCAGGATCAAAATCAGTT+GGG	0.555773	7.1:-48635162	None:intergenic
AGCCTGAAAACTCTTTCCGA+TGG	0.583898	7.1:-48635214	None:intergenic
TGGTTTCATAAATCCCACAT+TGG	0.595026	7.1:-48635323	None:intergenic
GGAGGCCCACGATGATCAGT+AGG	0.597332	7.1:-48635193	None:intergenic
TACTGATTTAGGTTTGAAGG+AGG	0.609454	7.1:+48635492	MS.gene027319:CDS
GAAGCCTAGGTCAAGCTGCA+AGG	0.610433	7.1:+48635089	MS.gene027319:CDS
CAAAATTAAGGTCATCAAGG+AGG	0.615684	7.1:+48635459	MS.gene027319:CDS
GCATCTCTAACTCTAGCTGA+AGG	0.625143	7.1:+48635250	MS.gene027319:CDS
TCAAGCTGCAAGGAAAGAGG+AGG	0.631410	7.1:+48635099	MS.gene027319:CDS
AATGTGGGATTTATGAAACC+AGG	0.639785	7.1:+48635325	MS.gene027319:CDS
GATAAAGGAGATGCCCAATG+TGG	0.647613	7.1:+48635309	MS.gene027319:CDS
AACATCAACAGAAGCCAAGG+AGG	0.649027	7.1:+48635378	MS.gene027319:CDS
ATAAAGGAGATGCCCAATGT+GGG	0.651305	7.1:+48635310	MS.gene027319:CDS
TTGATCCTACTGATCATCGT+GGG	0.654861	7.1:+48635188	MS.gene027319:CDS
TTTGATCCTACTGATCATCG+TGG	0.702613	7.1:+48635187	MS.gene027319:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTCAAATACAGTTTTTTC+TGG	-	chr7.1:48635412-48635431	None:intergenic	20.0%
!	ACACCTTCTATTATAAAGAA+AGG	+	chr7.1:48635535-48635554	MS.gene027319:CDS	25.0%
!	ACACCTTTCTTTATAATAGA+AGG	-	chr7.1:48635541-48635560	None:intergenic	25.0%
!	TGTCTATACAACAAACTATT+GGG	-	chr7.1:48635013-48635032	None:intergenic	25.0%
!	TTGTCTATACAACAAACTAT+TGG	-	chr7.1:48635014-48635033	None:intergenic	25.0%
!!!	TTTTACTGATTTAGGTTTGA+AGG	+	chr7.1:48635489-48635508	MS.gene027319:CDS	25.0%
	AATGTATTTACAAGAAGCCT+AGG	+	chr7.1:48635076-48635095	MS.gene027319:CDS	30.0%
	ATAGAGAAATTGAAAGCTCT+AGG	+	chr7.1:48635586-48635605	MS.gene027319:CDS	30.0%
	GATGAAAAAAATGGGGATAA+AGG	+	chr7.1:48635294-48635313	MS.gene027319:CDS	30.0%
	TTCCAAAATTAAGGTCATCA+AGG	+	chr7.1:48635456-48635475	MS.gene027319:CDS	30.0%
!!	TATAAAGAAAGGTGTTTCGA+AGG	+	chr7.1:48635546-48635565	MS.gene027319:CDS	30.0%
!!	TGAGCTTGCTTTTAAGAATA+AGG	+	chr7.1:48634978-48634997	MS.gene027319:CDS	30.0%
	AAAAACATCAACAGAAGCCA+AGG	+	chr7.1:48635375-48635394	MS.gene027319:CDS	35.0%
	AATGTGGGATTTATGAAACC+AGG	+	chr7.1:48635325-48635344	MS.gene027319:CDS	35.0%
	ATCTGTAAATTTGGCTGGAA+TGG	+	chr7.1:48635348-48635367	MS.gene027319:CDS	35.0%
	CAAAATTAAGGTCATCAAGG+AGG	+	chr7.1:48635459-48635478	MS.gene027319:CDS	35.0%
	CTCGCTCTGATGAAAAAAAT+GGG	+	chr7.1:48635286-48635305	MS.gene027319:CDS	35.0%
	GAAACCAGGATCTGTAAATT+TGG	+	chr7.1:48635339-48635358	MS.gene027319:CDS	35.0%
	GGTTTCATAAATCCCACATT+GGG	-	chr7.1:48635325-48635344	None:intergenic	35.0%
	TCGCTCTGATGAAAAAAATG+GGG	+	chr7.1:48635287-48635306	MS.gene027319:CDS	35.0%
	TCTCGCTCTGATGAAAAAAA+TGG	+	chr7.1:48635285-48635304	MS.gene027319:CDS	35.0%
	TGAAGCAGCTTCCAAAATTA+AGG	+	chr7.1:48635447-48635466	MS.gene027319:CDS	35.0%
	TGGTTTCATAAATCCCACAT+TGG	-	chr7.1:48635326-48635345	None:intergenic	35.0%
	TTGCAGGATCAAAATCAGTT+GGG	-	chr7.1:48635165-48635184	None:intergenic	35.0%
!!	AATTTTGGAAGCTGCTTCAT+AGG	-	chr7.1:48635446-48635465	None:intergenic	35.0%
!!	CTCCTTGATGACCTTAATTT+TGG	-	chr7.1:48635461-48635480	None:intergenic	35.0%
!!	TACTGATTTAGGTTTGAAGG+AGG	+	chr7.1:48635492-48635511	MS.gene027319:CDS	35.0%
!!!	GTTCGAGGTTTTACTGATTT+AGG	+	chr7.1:48635481-48635500	MS.gene027319:CDS	35.0%
	ATAAAGGAGATGCCCAATGT+GGG	+	chr7.1:48635310-48635329	MS.gene027319:CDS	40.0%
	GTTGCAGGATCAAAATCAGT+TGG	-	chr7.1:48635166-48635185	None:intergenic	40.0%
	TCAGTAGGATCAAATGTTGC+AGG	-	chr7.1:48635181-48635200	None:intergenic	40.0%
	TTGATCCTACTGATCATCGT+GGG	+	chr7.1:48635188-48635207	MS.gene027319:CDS	40.0%
	TTTGATCCTACTGATCATCG+TGG	+	chr7.1:48635187-48635206	MS.gene027319:CDS	40.0%
!	TTTAGGTTTGAAGGAGGCAA+AGG	+	chr7.1:48635498-48635517	MS.gene027319:CDS	40.0%
!!	AAAGGTGTTTCGAAGGAAGA+AGG	+	chr7.1:48635553-48635572	MS.gene027319:CDS	40.0%
!!	TTTCAGGCTTGTTGATGAAG+TGG	+	chr7.1:48635228-48635247	MS.gene027319:CDS	40.0%
	AACATCAACAGAAGCCAAGG+AGG	+	chr7.1:48635378-48635397	MS.gene027319:CDS	45.0%
	AGCCTGAAAACTCTTTCCGA+TGG	-	chr7.1:48635217-48635236	None:intergenic	45.0%
	CCAGCCAAATTTACAGATCC+TGG	-	chr7.1:48635346-48635365	None:intergenic	45.0%
	CCAGGATCTGTAAATTTGGC+TGG	+	chr7.1:48635343-48635362	MS.gene027319:CDS	45.0%
	CTCTTACTGCTAGCACTCTA+GGG	+	chr7.1:48635035-48635054	MS.gene027319:CDS	45.0%
	CTGAAAACTCTTTCCGATGG+AGG	-	chr7.1:48635214-48635233	None:intergenic	45.0%
	GAAGGAGGCAAAGGATTTAG+TGG	+	chr7.1:48635507-48635526	MS.gene027319:CDS	45.0%
	GATAAAGGAGATGCCCAATG+TGG	+	chr7.1:48635309-48635328	MS.gene027319:CDS	45.0%
	GCATCTCTAACTCTAGCTGA+AGG	+	chr7.1:48635250-48635269	MS.gene027319:CDS	45.0%
	TCTAGGCGCAATTGTCGTTA+TGG	+	chr7.1:48635603-48635622	MS.gene027319:CDS	45.0%
!	CTAGCTGAAGGTGCAGAATT+GGG	+	chr7.1:48635262-48635281	MS.gene027319:CDS	45.0%
!	CTCCATCGGAAAGAGTTTTC+AGG	+	chr7.1:48635212-48635231	MS.gene027319:CDS	45.0%
!	TCTAGCTGAAGGTGCAGAAT+TGG	+	chr7.1:48635261-48635280	MS.gene027319:CDS	45.0%
	AAGGTCATCAAGGAGGTTCG+AGG	+	chr7.1:48635466-48635485	MS.gene027319:CDS	50.0%
	AGGTCAAGCTGCAAGGAAAG+AGG	+	chr7.1:48635096-48635115	MS.gene027319:CDS	50.0%
	CCTCTTACTGCTAGCACTCT+AGG	+	chr7.1:48635034-48635053	MS.gene027319:CDS	50.0%
	CTTTCCTTGCAGCTTGACCT+AGG	-	chr7.1:48635096-48635115	None:intergenic	50.0%
	TCAAGCTGCAAGGAAAGAGG+AGG	+	chr7.1:48635099-48635118	MS.gene027319:CDS	50.0%
!	CCTAGAGTGCTAGCAGTAAG+AGG	-	chr7.1:48635037-48635056	None:intergenic	50.0%
!!!	TTCTGGCTTTTGCTCCTCCT+TGG	-	chr7.1:48635395-48635414	None:intergenic	50.0%
	AGCTGCAAGGAAAGAGGAGG+AGG	+	chr7.1:48635102-48635121	MS.gene027319:CDS	55.0%
	GAAGCCTAGGTCAAGCTGCA+AGG	+	chr7.1:48635089-48635108	MS.gene027319:CDS	55.0%
	TGATCATCGTGGGCCTCCAT+CGG	+	chr7.1:48635198-48635217	MS.gene027319:CDS	55.0%
	GGAGGCCCACGATGATCAGT+AGG	-	chr7.1:48635196-48635215	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	48634977	48635630	48634977	ID=MS.gene027319
chr7.1	mRNA	48634977	48635630	48634977	ID=MS.gene027319.t1;Parent=MS.gene027319
chr7.1	exon	48634977	48635630	48634977	ID=MS.gene027319.t1.exon1;Parent=MS.gene027319.t1
chr7.1	CDS	48634977	48635630	48634977	ID=cds.MS.gene027319.t1;Parent=MS.gene027319.t1

Gene Sequence

>MS.gene027319

ATGAGCTTGCTTTTAAGAATAAGGCATTGTTTACCCAATAGTTTGTTGTATAGACAACCTCTTACTGCTAGCACTCTAGGGCTTAATGCTAGAACATTAAATGTATTTACAAGAAGCCTAGGTCAAGCTGCAAGGAAAGAGGAGGAGGAAGATGTAGAAGAAGTTGAAATTGATCAACGAAGTCTCCCAACTGATTTTGATCCTGCAACATTTGATCCTACTGATCATCGTGGGCCTCCATCGGAAAGAGTTTTCAGGCTTGTTGATGAAGTGGCATCTCTAACTCTAGCTGAAGGTGCAGAATTGGGTCTCGCTCTGATGAAAAAAATGGGGATAAAGGAGATGCCCAATGTGGGATTTATGAAACCAGGATCTGTAAATTTGGCTGGAATGGCAGCAAAAACATCAACAGAAGCCAAGGAGGAGCAAAAGCCAGAAAAAACTGTATTTGAATTGAAACTGACGTCCTATGAAGCAGCTTCCAAAATTAAGGTCATCAAGGAGGTTCGAGGTTTTACTGATTTAGGTTTGAAGGAGGCAAAGGATTTAGTGGAAAAAACACCTTCTATTATAAAGAAAGGTGTTTCGAAGGAAGAAGGAGAGCAGATAATAGAGAAATTGAAAGCTCTAGGCGCAATTGTCGTTATGGAATGA

Protein sequence

>MS.gene027319.t1

MSLLLRIRHCLPNSLLYRQPLTASTLGLNARTLNVFTRSLGQAARKEEEEDVEEVEIDQRSLPTDFDPATFDPTDHRGPPSERVFRLVDEVASLTLAEGAELGLALMKKMGIKEMPNVGFMKPGSVNLAGMAAKTSTEAKEEQKPEKTVFELKLTSYEAASKIKVIKEVRGFTDLGLKEAKDLVEKTPSIIKKGVSKEEGEQIIEKLKALGAIVVME