AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028182

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028182.t1 MTR_5g019460 44.218 294 148 6 47 329 77 365 2.73e-78 244
MS.gene028182.t1 MTR_8g078070 41.695 295 152 8 48 329 79 366 5.98e-74 233
MS.gene028182.t1 MTR_5g092910 31.674 221 133 4 53 263 46 258 3.29e-24 100
MS.gene028182.t1 MTR_2g072470 25.116 215 144 6 61 268 113 317 4.95e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028182.t1 AT4G38460 64.605 291 93 2 45 335 42 322 1.24e-130 376
MS.gene028182.t1 AT4G36810 44.554 303 148 7 42 329 68 365 7.46e-82 253
MS.gene028182.t1 AT2G18620 39.093 353 187 9 1 333 1 345 3.52e-81 251
MS.gene028182.t1 AT2G18640 43.333 300 150 10 48 334 79 371 2.21e-71 226
MS.gene028182.t1 AT2G23800 40.667 300 158 10 48 334 83 375 2.05e-66 214
MS.gene028182.t1 AT3G20160 39.677 310 160 9 43 333 41 342 4.05e-66 212
MS.gene028182.t1 AT3G14550 42.424 297 150 7 48 329 64 354 5.62e-64 207
MS.gene028182.t1 AT3G14550 42.424 297 150 7 48 329 64 354 5.62e-64 207
MS.gene028182.t1 AT3G14550 42.424 297 150 7 48 329 64 354 5.62e-64 207
MS.gene028182.t1 AT3G20160 39.535 301 145 9 43 333 41 314 1.36e-63 204
MS.gene028182.t1 AT3G14530 42.761 297 149 7 48 329 64 354 6.84e-63 204
MS.gene028182.t1 AT3G14510 42.599 277 138 7 68 329 8 278 1.28e-62 201
MS.gene028182.t1 AT3G29430 41.231 325 166 11 24 329 33 351 9.48e-62 201
MS.gene028182.t1 AT3G32040 42.712 295 152 7 48 329 64 354 5.46e-61 199
MS.gene028182.t1 AT1G49530 39.672 305 157 8 52 333 34 334 7.73e-60 196
MS.gene028182.t1 AT1G17050 26.490 151 102 3 119 268 171 313 1.18e-11 65.9

Find 119 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGTTATGTAGAGGTTTA+TGG 0.097267 4.2:-16035770 MS.gene028182:CDS
TTGGATGGATTTGAGAAGTA+TGG 0.258489 4.2:-16035692 MS.gene028182:CDS
AGCAAAGGCTTTGGAGGAAT+TGG 0.259488 4.2:-16035711 MS.gene028182:CDS
CGGTGTTGACATGGCAATTC+TGG 0.263672 4.2:-16036167 MS.gene028182:CDS
TGCTATTGTAGATGATATTT+TGG 0.266530 4.2:-16035840 MS.gene028182:CDS
TGTATCAATCCAACTGCATT+TGG 0.293189 4.2:+16035983 None:intergenic
TCTGATTAATCTGCCCCATT+AGG 0.299471 4.2:+16037042 None:intergenic
GGCTCCACTGGAATGGCTGC+AGG 0.305044 4.2:-16036031 MS.gene028182:CDS
AATGGCTGCAGGGCAGTTCT+TGG 0.308107 4.2:-16036020 MS.gene028182:CDS
AGAGTTATGTAGAGGTTTAT+GGG 0.313757 4.2:-16035769 MS.gene028182:CDS
GGCTGCTCCTTCTCCTTCTT+CGG 0.326678 4.2:-16037117 MS.gene028182:CDS
GGCATTGTCGCAATTGTAAA+AGG 0.335180 4.2:+16037203 None:intergenic
GGGCAGTTCTTGGACCTTGA+AGG 0.335310 4.2:-16036010 MS.gene028182:CDS
CGTAATTCACAAAACTATAG+AGG 0.352365 4.2:+16035654 None:intergenic
GGCAGTTCTTGGACCTTGAA+GGG 0.352960 4.2:-16036009 MS.gene028182:CDS
CTGATTAATCTGCCCCATTA+GGG 0.371204 4.2:+16037043 None:intergenic
GGATGAGATTCGGAGACTTT+CGG 0.384911 4.2:+16037159 None:intergenic
TGCCATGTCAACACCGTAGA+TGG 0.385514 4.2:+16036174 None:intergenic
TTTCCCATCTTTATCTCCCT+CGG 0.385637 4.2:+16035802 None:intergenic
CCGTAGATGGCGTGGTTTGA+GGG 0.385791 4.2:+16036187 None:intergenic
CAAGCAGAGATACACATAAC+AGG 0.399894 4.2:+16036927 None:intergenic
GACGTATTGGACTTCCCTAA+TGG 0.407597 4.2:-16037057 MS.gene028182:CDS
ACTTTGATTTGAAGACGTAT+TGG 0.408292 4.2:-16037070 MS.gene028182:CDS
ACACGAAGTACACGGGGTTC+AGG 0.414466 4.2:+16036079 None:intergenic
GTATCAATCCAACTGCATTT+GGG 0.418171 4.2:+16035984 None:intergenic
ATAGAGAGACTGAGGAGGTA+TGG 0.421597 4.2:-16035884 MS.gene028182:CDS
AGAACTTAGAGCAAAGGCTT+TGG 0.423144 4.2:-16035720 MS.gene028182:CDS
GCCCGCTGTGTGGGCTCCAC+TGG 0.424889 4.2:-16036043 MS.gene028182:CDS
ACGTATTGGACTTCCCTAAT+GGG 0.427242 4.2:-16037056 MS.gene028182:CDS
GAAAGAAGATTACCATTTCA+AGG 0.432327 4.2:+16036856 None:intergenic
GCAGCCATGGAGCAGCGAAT+TGG 0.434085 4.2:+16037134 None:intergenic
GGTGATGCACTCTTTCCCCT+TGG 0.434286 4.2:-16036142 MS.gene028182:CDS
TGCAGTGTGTGGAGGGTTGT+TGG 0.437777 4.2:-16035927 MS.gene028182:CDS
AGATGGCGTGGTTTGAGGGC+TGG 0.460200 4.2:+16036191 None:intergenic
ACTTAGAGCAAAGGCTTTGG+AGG 0.466634 4.2:-16035717 MS.gene028182:CDS
CGAATTGGAAGGATGAGATT+CGG 0.467097 4.2:+16037149 None:intergenic
TTGAGGGAACTGAACTTCAA+TGG 0.473114 4.2:+16037007 None:intergenic
CTGTGTGGGCTCCACTGGAA+TGG 0.474784 4.2:-16036038 MS.gene028182:CDS
TCTCTCTATCTCATCATCTT+CGG 0.478860 4.2:+16035898 None:intergenic
GCAGTTCTTGGACCTTGAAG+GGG 0.479193 4.2:-16036008 MS.gene028182:CDS
CACTGCATGTGCCCTTGAAA+TGG 0.480852 4.2:-16036868 MS.gene028182:intron
TTCCCATCTTTATCTCCCTC+GGG 0.481004 4.2:+16035803 None:intergenic
CGGTATTCTGCCCTTGCTAA+AGG 0.483034 4.2:-16036966 MS.gene028182:CDS
CAATGCCCTCATTAAACATC+GGG 0.486811 4.2:-16037187 MS.gene028182:CDS
TCCAGTGGAGCCCACACAGC+GGG 0.487936 4.2:+16036042 None:intergenic
TTGACGAGGCCAGTAGATGA+TGG 0.492007 4.2:-16035610 None:intergenic
ACCGTAGATGGCGTGGTTTG+AGG 0.494704 4.2:+16036186 None:intergenic
CCCTCAAACCACGCCATCTA+CGG 0.495109 4.2:-16036187 MS.gene028182:CDS
AGAAGGAGAAGGAGCAGCCA+TGG 0.495191 4.2:+16037121 None:intergenic
AGGAGGTATGGTAGAGCTGT+TGG 0.495677 4.2:-16035872 MS.gene028182:CDS
CGTGGTTTGAGGGCTGGCCA+CGG 0.508134 4.2:+16036197 None:intergenic
CAGTTCTTGGACCTTGAAGG+GGG 0.508920 4.2:-16036007 MS.gene028182:CDS
AAACCCGAGGGAGATAAAGA+TGG 0.509232 4.2:-16035806 MS.gene028182:CDS
CCTTCCAATTCGCTGCTCCA+TGG 0.514052 4.2:-16037138 MS.gene028182:CDS
GTGTGTGGAGGGTTGTTGGC+CGG 0.518361 4.2:-16035923 MS.gene028182:CDS
ATCGCTTAGCACCTTTAGCA+AGG 0.521626 4.2:+16036955 None:intergenic
TATCTCATCATCTTCGGCAC+CGG 0.522001 4.2:+16035904 None:intergenic
CGCCATCTACGGTGTTGACA+TGG 0.522018 4.2:-16036176 MS.gene028182:CDS
ATGATGAGATAGAGAGACTG+AGG 0.525344 4.2:-16035892 MS.gene028182:CDS
TTCAAGAACCCATCATCTAC+TGG 0.527028 4.2:+16035601 None:intergenic
GCTCCACTGGAATGGCTGCA+GGG 0.527665 4.2:-16036030 MS.gene028182:CDS
GTAATTCACAAAACTATAGA+GGG 0.531875 4.2:+16035655 None:intergenic
GGGCTGGCCACGGCGTGACA+AGG 0.532295 4.2:+16036207 None:intergenic
TGACAAGGAGTCATCCATGC+AGG 0.533253 4.2:+16036222 None:intergenic
ATAGCTTCATATATCTGTTG+AGG 0.536580 4.2:+16036990 None:intergenic
CAGAGGGAAGAGTTATGTAG+AGG 0.537549 4.2:-16035777 MS.gene028182:CDS
GATCGGATGGAAACCGAAGA+AGG 0.542003 4.2:+16037104 None:intergenic
GAAACACCCGATGTTTAATG+AGG 0.542457 4.2:+16037181 None:intergenic
GAGTTATGTAGAGGTTTATG+GGG 0.542842 4.2:-16035768 MS.gene028182:CDS
AACAATGTGTTGAAATCCAA+GGG 0.546111 4.2:+16036126 None:intergenic
CATTTCAAGGGCACATGCAG+TGG 0.547793 4.2:+16036869 None:intergenic
TAGCTTCATATATCTGTTGA+GGG 0.547993 4.2:+16036991 None:intergenic
AAGGCTTTGGAGGAATTGGA+TGG 0.548932 4.2:-16035707 MS.gene028182:CDS
GAGTGTTCTGCAGTGTGTGG+AGG 0.552684 4.2:-16035935 MS.gene028182:CDS
AAGGGCACATGCAGTGGGGA+AGG 0.558957 4.2:+16036875 None:intergenic
AAAGAAGATTACCATTTCAA+GGG 0.560180 4.2:+16036857 None:intergenic
GTACACGGGGTTCAGGTACA+AGG 0.565661 4.2:+16036086 None:intergenic
ATCATCATGTATCAATGAAG+CGG 0.568247 4.2:+16036249 None:intergenic
ATGCAGTGGGGAAGGCAGCA+AGG 0.568393 4.2:+16036883 None:intergenic
ATGAGATAGAGAGACTGAGG+AGG 0.573192 4.2:-16035889 MS.gene028182:CDS
AACCCGAGGGAGATAAAGAT+GGG 0.574299 4.2:-16035805 MS.gene028182:CDS
ATTTCAAGGGCACATGCAGT+GGG 0.576737 4.2:+16036870 None:intergenic
CAGCAATGACACGAAGTACA+CGG 0.576879 4.2:+16036071 None:intergenic
AGTGTTCTGCAGTGTGTGGA+GGG 0.579045 4.2:-16035934 MS.gene028182:CDS
GGCTGAAGAACTTAGAGCAA+AGG 0.579804 4.2:-16035726 MS.gene028182:CDS
CTGCCCTGCAGCCATTCCAG+TGG 0.580181 4.2:+16036027 None:intergenic
GCTGAGATAGCCCGCTGTGT+GGG 0.580286 4.2:-16036052 MS.gene028182:CDS
AGCAATGACACGAAGTACAC+GGG 0.582072 4.2:+16036072 None:intergenic
GTTGACATGGCAATTCTGGC+GGG 0.592172 4.2:-16036163 MS.gene028182:CDS
TCGCTTAGCACCTTTAGCAA+GGG 0.604739 4.2:+16036956 None:intergenic
CCATGGAGCAGCGAATTGGA+AGG 0.607622 4.2:+16037138 None:intergenic
ACATGATGATCTTCCCTGCA+TGG 0.608437 4.2:-16036236 MS.gene028182:CDS
ACAATGCCCTCATTAAACAT+CGG 0.612387 4.2:-16037188 MS.gene028182:CDS
GGGTTCAGGTACAAGGTCAG+AGG 0.613058 4.2:+16036093 None:intergenic
ATGGAAACCGAAGAAGGAGA+AGG 0.615829 4.2:+16037110 None:intergenic
TGTTGACATGGCAATTCTGG+CGG 0.616197 4.2:-16036164 MS.gene028182:CDS
AAACAATGTGTTGAAATCCA+AGG 0.617990 4.2:+16036125 None:intergenic
AAGCAGAGATACACATAACA+GGG 0.618552 4.2:+16036928 None:intergenic
GATGACTCCTTGTCACGCCG+TGG 0.624688 4.2:-16036214 MS.gene028182:CDS
GACAAGGAGTCATCCATGCA+GGG 0.630249 4.2:+16036223 None:intergenic
GCGGAGTGTTCTGCAGTGTG+TGG 0.632086 4.2:-16035938 MS.gene028182:CDS
GGAAGAGAGACTGAAACCCG+AGG 0.632492 4.2:-16035819 MS.gene028182:CDS
TGCTGAGATAGCCCGCTGTG+TGG 0.636542 4.2:-16036053 MS.gene028182:CDS
AAACACCCGATGTTTAATGA+GGG 0.640367 4.2:+16037182 None:intergenic
TGTCAACACCGTAGATGGCG+TGG 0.643803 4.2:+16036179 None:intergenic
GCAGAGATACACATAACAGG+GGG 0.650726 4.2:+16036930 None:intergenic
AGAGATACACATAACAGGGG+GGG 0.650807 4.2:+16036932 None:intergenic
TAGAAGTTTCAATGTTGACG+AGG 0.655023 4.2:-16035624 MS.gene028182:CDS
CGTATTGGACTTCCCTAATG+GGG 0.660461 4.2:-16037055 MS.gene028182:CDS
GAAGAGAGACTGAAACCCGA+GGG 0.662153 4.2:-16035818 MS.gene028182:CDS
GGTTCAGGTACAAGGTCAGA+GGG 0.665434 4.2:+16036094 None:intergenic
AACAGATATATGAAGCTATG+CGG 0.703508 4.2:-16036986 MS.gene028182:CDS
TAATTCACAAAACTATAGAG+GGG 0.709745 4.2:+16035656 None:intergenic
AGCAGAGATACACATAACAG+GGG 0.710591 4.2:+16036929 None:intergenic
TTCCAGTGGAGCCCACACAG+CGG 0.722527 4.2:+16036041 None:intergenic
ACAATGTGTTGAAATCCAAG+GGG 0.726426 4.2:+16036127 None:intergenic
CAGAGATACACATAACAGGG+GGG 0.735594 4.2:+16036931 None:intergenic
TTTCAAGGGCACATGCAGTG+GGG 0.742785 4.2:+16036871 None:intergenic
GCAATGACACGAAGTACACG+GGG 0.769728 4.2:+16036073 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGTTTGGTATTTCTTAT+TGG - chr4.2:16036431-16036450 MS.gene028182:intron 20.0%
!! TTGATACAAGATAAAAAGTT+TGG - chr4.2:16036854-16036873 MS.gene028182:intron 20.0%
!!! ATGATAAACTGTATGAATTT+TGG - chr4.2:16036519-16036538 MS.gene028182:intron 20.0%
! AAAGAAGATTACCATTTCAA+GGG + chr4.2:16035968-16035987 None:intergenic 25.0%
! AATATTCAGAGACACATTTA+AGG + chr4.2:16036412-16036431 None:intergenic 25.0%
! AATCAAAATTGGAAAGAGAA+AGG + chr4.2:16036196-16036215 None:intergenic 25.0%
! ACAATAGAATGAATATGAAG+TGG + chr4.2:16036001-16036020 None:intergenic 25.0%
! GTAATTCACAAAACTATAGA+GGG + chr4.2:16037170-16037189 None:intergenic 25.0%
! TAATTCACAAAACTATAGAG+GGG + chr4.2:16037169-16037188 None:intergenic 25.0%
! TGTCTCTGAATATTTATGTT+TGG - chr4.2:16036418-16036437 MS.gene028182:intron 25.0%
!! AGATAAAAAGTTTGGTGAAA+TGG - chr4.2:16036862-16036881 MS.gene028182:intron 25.0%
!! TGCTATTGTAGATGATATTT+TGG - chr4.2:16036982-16037001 MS.gene028182:CDS 25.0%
!!! ATAGTACATACTTTGACTTT+TGG - chr4.2:16036357-16036376 MS.gene028182:intron 25.0%
AAACAATGTGTTGAAATCCA+AGG + chr4.2:16036700-16036719 None:intergenic 30.0%
AACAATGTGTTGAAATCCAA+GGG + chr4.2:16036699-16036718 None:intergenic 30.0%
AACAGATATATGAAGCTATG+CGG - chr4.2:16035836-16035855 MS.gene028182:CDS 30.0%
AAGAGTTATGTAGAGGTTTA+TGG - chr4.2:16037052-16037071 MS.gene028182:CDS 30.0%
AGAGTTATGTAGAGGTTTAT+GGG - chr4.2:16037053-16037072 MS.gene028182:CDS 30.0%
ATAAAGATGGGAAAAACAGA+GGG - chr4.2:16037029-16037048 MS.gene028182:CDS 30.0%
ATAGCTTCATATATCTGTTG+AGG + chr4.2:16035835-16035854 None:intergenic 30.0%
ATCATCATGTATCAATGAAG+CGG + chr4.2:16036576-16036595 None:intergenic 30.0%
CAGATACAACTAGCTAAAAA+TGG + chr4.2:16036331-16036350 None:intergenic 30.0%
CGTAATTCACAAAACTATAG+AGG + chr4.2:16037171-16037190 None:intergenic 30.0%
GAAAGAAGATTACCATTTCA+AGG + chr4.2:16035969-16035988 None:intergenic 30.0%
GATAGGTAAAAGTAAAACTC+TGG + chr4.2:16036113-16036132 None:intergenic 30.0%
TAGCTTCATATATCTGTTGA+GGG + chr4.2:16035834-16035853 None:intergenic 30.0%
! TAAAAAGTTTGGTGAAATGG+CGG - chr4.2:16036865-16036884 MS.gene028182:intron 30.0%
! TAGGAACGGATTTATTTGAT+AGG + chr4.2:16036130-16036149 None:intergenic 30.0%
!! ACTTTGATTTGAAGACGTAT+TGG - chr4.2:16035752-16035771 MS.gene028182:CDS 30.0%
!!! CTTATATTTTAGTTGCAAGC+AGG - chr4.2:16036544-16036563 MS.gene028182:intron 30.0%
AAACACCCGATGTTTAATGA+GGG + chr4.2:16035643-16035662 None:intergenic 35.0%
AAGCAGAGATACACATAACA+GGG + chr4.2:16035897-16035916 None:intergenic 35.0%
ACAATGCCCTCATTAAACAT+CGG - chr4.2:16035634-16035653 MS.gene028182:CDS 35.0%
ACAATGTGTTGAAATCCAAG+GGG + chr4.2:16036698-16036717 None:intergenic 35.0%
GAGTTATGTAGAGGTTTATG+GGG - chr4.2:16037054-16037073 MS.gene028182:CDS 35.0%
GATAAAGATGGGAAAAACAG+AGG - chr4.2:16037028-16037047 MS.gene028182:CDS 35.0%
GTATCAATCCAACTGCATTT+GGG + chr4.2:16036841-16036860 None:intergenic 35.0%
TAGAAGTTTCAATGTTGACG+AGG - chr4.2:16037198-16037217 MS.gene028182:CDS 35.0%
TCTCTCTATCTCATCATCTT+CGG + chr4.2:16036927-16036946 None:intergenic 35.0%
TGTATCAATCCAACTGCATT+TGG + chr4.2:16036842-16036861 None:intergenic 35.0%
! ATCTCTGCTTGTGAACTTTT+CGG - chr4.2:16035907-16035926 MS.gene028182:CDS 35.0%
! CAAAGTGAACAGCTTTTGAT+CGG + chr4.2:16035738-16035757 None:intergenic 35.0%
! GGATGAGCTTTCTTTTTCTT+TGG - chr4.2:16036166-16036185 MS.gene028182:CDS 35.0%
! TTGGATGGATTTGAGAAGTA+TGG - chr4.2:16037130-16037149 MS.gene028182:CDS 35.0%
AAAGAAAGCTCATCCTCTCA+CGG + chr4.2:16036161-16036180 None:intergenic 40.0%
ACGTATTGGACTTCCCTAAT+GGG - chr4.2:16035766-16035785 MS.gene028182:CDS 40.0%
AGAACTTAGAGCAAAGGCTT+TGG - chr4.2:16037102-16037121 MS.gene028182:CDS 40.0%
AGCAGAGATACACATAACAG+GGG + chr4.2:16035896-16035915 None:intergenic 40.0%
ATGATGAGATAGAGAGACTG+AGG - chr4.2:16036930-16036949 MS.gene028182:CDS 40.0%
CAAGCAGAGATACACATAAC+AGG + chr4.2:16035898-16035917 None:intergenic 40.0%
CAATGCCCTCATTAAACATC+GGG - chr4.2:16035635-16035654 MS.gene028182:CDS 40.0%
CGAATTGGAAGGATGAGATT+CGG + chr4.2:16035676-16035695 None:intergenic 40.0%
CTGATTAATCTGCCCCATTA+GGG + chr4.2:16035782-16035801 None:intergenic 40.0%
GAAACACCCGATGTTTAATG+AGG + chr4.2:16035644-16035663 None:intergenic 40.0%
GACAATGATAGACCAATCTC+TGG + chr4.2:16036061-16036080 None:intergenic 40.0%
GGCATTGTCGCAATTGTAAA+AGG + chr4.2:16035622-16035641 None:intergenic 40.0%
TCTGATTAATCTGCCCCATT+AGG + chr4.2:16035783-16035802 None:intergenic 40.0%
TTGAGGGAACTGAACTTCAA+TGG + chr4.2:16035818-16035837 None:intergenic 40.0%
TTTCCCATCTTTATCTCCCT+CGG + chr4.2:16037023-16037042 None:intergenic 40.0%
AAACCCGAGGGAGATAAAGA+TGG - chr4.2:16037016-16037035 MS.gene028182:CDS 45.0%
AACCCGAGGGAGATAAAGAT+GGG - chr4.2:16037017-16037036 MS.gene028182:CDS 45.0%
ACATGATGATCTTCCCTGCA+TGG - chr4.2:16036586-16036605 MS.gene028182:intron 45.0%
AGAGATACACATAACAGGGG+GGG + chr4.2:16035893-16035912 None:intergenic 45.0%
AGCAATGACACGAAGTACAC+GGG + chr4.2:16036753-16036772 None:intergenic 45.0%
ATAGAGAGACTGAGGAGGTA+TGG - chr4.2:16036938-16036957 MS.gene028182:CDS 45.0%
ATCGCTTAGCACCTTTAGCA+AGG + chr4.2:16035870-16035889 None:intergenic 45.0%
ATGAGATAGAGAGACTGAGG+AGG - chr4.2:16036933-16036952 MS.gene028182:CDS 45.0%
ATGGAAACCGAAGAAGGAGA+AGG + chr4.2:16035715-16035734 None:intergenic 45.0%
CAGAGATACACATAACAGGG+GGG + chr4.2:16035894-16035913 None:intergenic 45.0%
CAGAGGGAAGAGTTATGTAG+AGG - chr4.2:16037045-16037064 MS.gene028182:CDS 45.0%
CAGCAATGACACGAAGTACA+CGG + chr4.2:16036754-16036773 None:intergenic 45.0%
CGTATTGGACTTCCCTAATG+GGG - chr4.2:16035767-16035786 MS.gene028182:CDS 45.0%
GACGTATTGGACTTCCCTAA+TGG - chr4.2:16035765-16035784 MS.gene028182:CDS 45.0%
GCAGAGATACACATAACAGG+GGG + chr4.2:16035895-16035914 None:intergenic 45.0%
GGATGAGATTCGGAGACTTT+CGG + chr4.2:16035666-16035685 None:intergenic 45.0%
GGCTGAAGAACTTAGAGCAA+AGG - chr4.2:16037096-16037115 MS.gene028182:CDS 45.0%
TATCTCATCATCTTCGGCAC+CGG + chr4.2:16036921-16036940 None:intergenic 45.0%
TCGCTTAGCACCTTTAGCAA+GGG + chr4.2:16035869-16035888 None:intergenic 45.0%
TGTTGACATGGCAATTCTGG+CGG - chr4.2:16036658-16036677 MS.gene028182:intron 45.0%
TTCCCATCTTTATCTCCCTC+GGG + chr4.2:16037022-16037041 None:intergenic 45.0%
! ACTTAGAGCAAAGGCTTTGG+AGG - chr4.2:16037105-16037124 MS.gene028182:CDS 45.0%
! ATTTCAAGGGCACATGCAGT+GGG + chr4.2:16035955-16035974 None:intergenic 45.0%
! GTGAACAGCTTTTGATCGGA+TGG + chr4.2:16035734-16035753 None:intergenic 45.0%
! TCTGCTTGTGAACTTTTCGG+AGG - chr4.2:16035910-16035929 MS.gene028182:CDS 45.0%
!! AAGGCTTTGGAGGAATTGGA+TGG - chr4.2:16037115-16037134 MS.gene028182:CDS 45.0%
!! AGCAAAGGCTTTGGAGGAAT+TGG - chr4.2:16037111-16037130 MS.gene028182:CDS 45.0%
!!! TTATTTTAATAAATCAAAAT+TGG + chr4.2:16036207-16036226 None:intergenic 5.0%
AAAGCTCATCCTCTCACGGT+AGG + chr4.2:16036157-16036176 None:intergenic 50.0%
AGTGTTCTGCAGTGTGTGGA+GGG - chr4.2:16036888-16036907 MS.gene028182:CDS 50.0%
CACTGCATGTGCCCTTGAAA+TGG - chr4.2:16035954-16035973 MS.gene028182:CDS 50.0%
CAGTTCTTGGACCTTGAAGG+GGG - chr4.2:16036815-16036834 MS.gene028182:intron 50.0%
CGGTATTCTGCCCTTGCTAA+AGG - chr4.2:16035856-16035875 MS.gene028182:CDS 50.0%
GAAGAGAGACTGAAACCCGA+GGG - chr4.2:16037004-16037023 MS.gene028182:CDS 50.0%
GACAAGGAGTCATCCATGCA+GGG + chr4.2:16036602-16036621 None:intergenic 50.0%
GATCGGATGGAAACCGAAGA+AGG + chr4.2:16035721-16035740 None:intergenic 50.0%
GCAATGACACGAAGTACACG+GGG + chr4.2:16036752-16036771 None:intergenic 50.0%
GCAGTTCTTGGACCTTGAAG+GGG - chr4.2:16036814-16036833 MS.gene028182:intron 50.0%
GCTAAGAACTCGCCAGAGAT+TGG - chr4.2:16036046-16036065 MS.gene028182:CDS 50.0%
GGCAGTTCTTGGACCTTGAA+GGG - chr4.2:16036813-16036832 MS.gene028182:intron 50.0%
GGTTCAGGTACAAGGTCAGA+GGG + chr4.2:16036731-16036750 None:intergenic 50.0%
GGTTGAAAAAGCAGCAGAGG+TGG - chr4.2:16037075-16037094 MS.gene028182:CDS 50.0%
GTTGACATGGCAATTCTGGC+GGG - chr4.2:16036659-16036678 MS.gene028182:intron 50.0%
TCACGGTAGGCAGTTAGGAA+CGG + chr4.2:16036144-16036163 None:intergenic 50.0%
TGACAAGGAGTCATCCATGC+AGG + chr4.2:16036603-16036622 None:intergenic 50.0%
TGCCATGTCAACACCGTAGA+TGG + chr4.2:16036651-16036670 None:intergenic 50.0%
TGGGGTTGAAAAAGCAGCAG+AGG - chr4.2:16037072-16037091 MS.gene028182:CDS 50.0%
! AGGAGGTATGGTAGAGCTGT+TGG - chr4.2:16036950-16036969 MS.gene028182:CDS 50.0%
! CATTTCAAGGGCACATGCAG+TGG + chr4.2:16035956-16035975 None:intergenic 50.0%
! TTTCAAGGGCACATGCAGTG+GGG + chr4.2:16035954-16035973 None:intergenic 50.0%
!! CGGTGTTGACATGGCAATTC+TGG - chr4.2:16036655-16036674 MS.gene028182:intron 50.0%
AATGGCTGCAGGGCAGTTCT+TGG - chr4.2:16036802-16036821 MS.gene028182:intron 55.0%
ACACGAAGTACACGGGGTTC+AGG + chr4.2:16036746-16036765 None:intergenic 55.0%
ACCGTAGATGGCGTGGTTTG+AGG + chr4.2:16036639-16036658 None:intergenic 55.0%
AGAAGGAGAAGGAGCAGCCA+TGG + chr4.2:16035704-16035723 None:intergenic 55.0%
CCATGGAGCAGCGAATTGGA+AGG + chr4.2:16035687-16035706 None:intergenic 55.0%
CCCTCAAACCACGCCATCTA+CGG - chr4.2:16036635-16036654 MS.gene028182:intron 55.0%
CCGTAGATGGCGTGGTTTGA+GGG + chr4.2:16036638-16036657 None:intergenic 55.0%
CCTTCCAATTCGCTGCTCCA+TGG - chr4.2:16035684-16035703 MS.gene028182:CDS 55.0%
GAGTGTTCTGCAGTGTGTGG+AGG - chr4.2:16036887-16036906 MS.gene028182:CDS 55.0%
GGAAGAGAGACTGAAACCCG+AGG - chr4.2:16037003-16037022 MS.gene028182:CDS 55.0%
GGCTGCTCCTTCTCCTTCTT+CGG - chr4.2:16035705-16035724 MS.gene028182:CDS 55.0%
GGGCAGTTCTTGGACCTTGA+AGG - chr4.2:16036812-16036831 MS.gene028182:intron 55.0%
GGGTTCAGGTACAAGGTCAG+AGG + chr4.2:16036732-16036751 None:intergenic 55.0%
GGTGATGCACTCTTTCCCCT+TGG - chr4.2:16036680-16036699 MS.gene028182:intron 55.0%
GTACACGGGGTTCAGGTACA+AGG + chr4.2:16036739-16036758 None:intergenic 55.0%
TCCTAACTGCCTACCGTGAG+AGG - chr4.2:16036145-16036164 MS.gene028182:CDS 55.0%
TCCTCTCACGGTAGGCAGTT+AGG + chr4.2:16036149-16036168 None:intergenic 55.0%
TGCAGTGTGTGGAGGGTTGT+TGG - chr4.2:16036895-16036914 MS.gene028182:CDS 55.0%
TGTCAACACCGTAGATGGCG+TGG + chr4.2:16036646-16036665 None:intergenic 55.0%
!! CGCCATCTACGGTGTTGACA+TGG - chr4.2:16036646-16036665 MS.gene028182:intron 55.0%
AGATGGCGTGGTTTGAGGGC+TGG + chr4.2:16036634-16036653 None:intergenic 60.0%
ATGCAGTGGGGAAGGCAGCA+AGG + chr4.2:16035942-16035961 None:intergenic 60.0%
CTGCATTTGGGCCCCCTTCA+AGG + chr4.2:16036829-16036848 None:intergenic 60.0%
CTGTGTGGGCTCCACTGGAA+TGG - chr4.2:16036784-16036803 MS.gene028182:intron 60.0%
GAAGGGGGCCCAAATGCAGT+TGG - chr4.2:16036830-16036849 MS.gene028182:intron 60.0%
GATGACTCCTTGTCACGCCG+TGG - chr4.2:16036608-16036627 MS.gene028182:intron 60.0%
GCAGCCATGGAGCAGCGAAT+TGG + chr4.2:16035691-16035710 None:intergenic 60.0%
GCTCCACTGGAATGGCTGCA+GGG - chr4.2:16036792-16036811 MS.gene028182:intron 60.0%
GCTGAGATAGCCCGCTGTGT+GGG - chr4.2:16036770-16036789 MS.gene028182:intron 60.0%
GTGTGTGGAGGGTTGTTGGC+CGG - chr4.2:16036899-16036918 MS.gene028182:CDS 60.0%
TGCTGAGATAGCCCGCTGTG+TGG - chr4.2:16036769-16036788 MS.gene028182:intron 60.0%
TTCCAGTGGAGCCCACACAG+CGG + chr4.2:16036784-16036803 None:intergenic 60.0%
! AAGGGCACATGCAGTGGGGA+AGG + chr4.2:16035950-16035969 None:intergenic 60.0%
! GCGGAGTGTTCTGCAGTGTG+TGG - chr4.2:16036884-16036903 MS.gene028182:CDS 60.0%
CTGCCCTGCAGCCATTCCAG+TGG + chr4.2:16036798-16036817 None:intergenic 65.0%
GGCTCCACTGGAATGGCTGC+AGG - chr4.2:16036791-16036810 MS.gene028182:intron 65.0%
TCCAGTGGAGCCCACACAGC+GGG + chr4.2:16036783-16036802 None:intergenic 65.0%
!! CGTGGTTTGAGGGCTGGCCA+CGG + chr4.2:16036628-16036647 None:intergenic 65.0%
GCCCGCTGTGTGGGCTCCAC+TGG - chr4.2:16036779-16036798 MS.gene028182:intron 75.0%
!! GGGCTGGCCACGGCGTGACA+AGG + chr4.2:16036618-16036637 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 16035613 16037231 16035613 ID=MS.gene028182
chr4.2 mRNA 16035613 16037231 16035613 ID=MS.gene028182.t1;Parent=MS.gene028182
chr4.2 exon 16036869 16037231 16036869 ID=MS.gene028182.t1.exon1;Parent=MS.gene028182.t1
chr4.2 CDS 16036869 16037231 16036869 ID=cds.MS.gene028182.t1;Parent=MS.gene028182.t1
chr4.2 exon 16035613 16036278 16035613 ID=MS.gene028182.t1.exon2;Parent=MS.gene028182.t1
chr4.2 CDS 16035613 16036278 16035613 ID=cds.MS.gene028182.t1;Parent=MS.gene028182.t1
Gene Sequence

>MS.gene028182

ATGGCTCCTTTTACAATTGCGACAATGCCCTCATTAAACATCGGGTGTTTCCGAAAGTCTCCGAATCTCATCCTTCCAATTCGCTGCTCCATGGCTGCTCCTTCTCCTTCTTCGGTTTCCATCCGATCAAAAGCTGTTCACTTTGATTTGAAGACGTATTGGACTTCCCTAATGGGGCAGATTAATCAGAAGCTCGATGAAGCCATTGAAGTTCAGTTCCCTCAACAGATATATGAAGCTATGCGGTATTCTGCCCTTGCTAAAGGTGCTAAGCGATCCCCCCCTGTTATGTGTATCTCTGCTTGTGAACTTTTCGGAGGCAGTCGCCTTGCTGCCTTCCCCACTGCATGTGCCCTTGAAATGGTAATCTTCTTTCTATAATCTGCCACTTCATATTCATTCTATTGTATTATGTTTTTAGTTTGTTTTTTATGCTAAGAACTCGCCAGAGATTGGTCTATCATTGTCACAAATTTATTTGTTATGTTGTTGAGCTACCAGAGTTTTACTTTTACCTATCAAATAAATCCGTTCCTAACTGCCTACCGTGAGAGGATGAGCTTTCTTTTTCTTTGGACTTCCTTTCTCTTTCCAATTTTGATTTATTAAAATAATAACATATGTTGCATATTTCTTCAAAAAAAAAAAAAAAACATATGTTGCATGTTTAGTTGCAGTAATGCTGTGTTGCTCATATAAGTAACAGTTTTGCATTCCATTTTTAGCTAGTTGTATCTGCTTAAAATAGTACATACTTTGACTTTTGGATCATCTGTTAAGAATGATTGTAGCATTACCTTAAATGTGTCTCTGAATATTTATGTTTGGTATTTCTTATTGGACACAAAGTGAGATTTATGAAGCGTTTATATAATTATATGCACTTCAGATAAATAAATAATGATGATGATAAACTGTATGAATTTTGGAACTTATATTTTAGTTGCAAGCAGGTTCATGCCGCTTCATTGATACATGATGATCTTCCCTGCATGGATGACTCCTTGTCACGCCGTGGCCAGCCCTCAAACCACGCCATCTACGGTGTTGACATGGCAATTCTGGCGGGTGATGCACTCTTTCCCCTTGGATTTCAACACATTGTTTCACATACTCCCTCTGACCTTGTACCTGAACCCCGTGTACTTCGTGTCATTGCTGAGATAGCCCGCTGTGTGGGCTCCACTGGAATGGCTGCAGGGCAGTTCTTGGACCTTGAAGGGGGCCCAAATGCAGTTGGATTGATACAAGATAAAAAGTTTGGTGAAATGGCGGAGTGTTCTGCAGTGTGTGGAGGGTTGTTGGCCGGTGCCGAAGATGATGAGATAGAGAGACTGAGGAGGTATGGTAGAGCTGTTGGAGTATTGTATGCTATTGTAGATGATATTTTGGAAGAGAGACTGAAACCCGAGGGAGATAAAGATGGGAAAAACAGAGGGAAGAGTTATGTAGAGGTTTATGGGGTTGAAAAAGCAGCAGAGGTGGCTGAAGAACTTAGAGCAAAGGCTTTGGAGGAATTGGATGGATTTGAGAAGTATGGAGATCAAGTGTTGCCCCTCTATAGTTTTGTGAATTACGCTATTGATAGAAGTTTCAATGTTGACGAGGCCAGTAGATGA
Protein sequence

>MS.gene028182.t1

MAPFTIATMPSLNIGCFRKSPNLILPIRCSMAAPSPSSVSIRSKAVHFDLKTYWTSLMGQINQKLDEAIEVQFPQQIYEAMRYSALAKGAKRSPPVMCISACELFGGSRLAAFPTACALEMVHAASLIHDDLPCMDDSLSRRGQPSNHAIYGVDMAILAGDALFPLGFQHIVSHTPSDLVPEPRVLRVIAEIARCVGSTGMAAGQFLDLEGGPNAVGLIQDKKFGEMAECSAVCGGLLAGAEDDEIERLRRYGRAVGVLYAIVDDILEERLKPEGDKDGKNRGKSYVEVYGVEKAAEVAEELRAKALEELDGFEKYGDQVLPLYSFVNYAIDRSFNVDEASR