AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030311

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030311.t1 MTR_6g090585 97.706 218 5 0 1 218 1 218 4.31e-161 444
MS.gene030311.t1 MTR_6g090585 97.283 184 5 0 35 218 1 184 1.38e-135 378
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030311.t1 AT4G29170 76.415 212 50 0 1 212 1 212 9.79e-123 347
MS.gene030311.t1 AT4G29170 74.619 197 50 0 16 212 1 197 1.15e-111 318
MS.gene030311.t1 AT4G29170 79.739 153 31 0 1 153 1 153 1.51e-85 250

Find 46 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAGATGAATTTGACTATT+TGG 0.177522 6.2:+88661396 MS.gene030311:CDS
CGAATCACAAGATTTCTTTC+TGG 0.201715 6.2:+88657561 MS.gene030311:CDS
CTGACTAATCACACCCTTCT+TGG 0.326573 6.2:-88659175 None:intergenic
TTGCCTCTCAGGTATACTTC+TGG 0.329157 6.2:+88659728 MS.gene030311:intron
TATTTAATCAGCTACTGCTT+TGG 0.360341 6.2:-88661437 None:intergenic
TCCTAGCTGCGCTGGAAATC+AGG 0.370421 6.2:+88659756 MS.gene030311:CDS
TGACTAATCACACCCTTCTT+GGG 0.388123 6.2:-88659174 None:intergenic
CTCATTATGCTTCAGCTGAA+TGG 0.388609 6.2:-88660166 None:intergenic
GCTTAAGGAGCTTGAGAAGA+TGG 0.394802 6.2:+88659149 MS.gene030311:intron
TAAAGATGTTGTCCAAAGTT+TGG 0.424035 6.2:+88659203 MS.gene030311:CDS
GCTGAAGCATAATGAGTTGA+AGG 0.430393 6.2:+88660173 MS.gene030311:CDS
GCAATGTGAGGCACTAAAGA+AGG 0.431414 6.2:+88659987 MS.gene030311:CDS
TTGATTCAGTCGAAGAAAAG+AGG 0.470370 6.2:+88657493 MS.gene030311:intron
CTCACATTGCTCGACAAGTT+GGG 0.470737 6.2:-88659974 None:intergenic
CTCATTGCTTCAAAGGCAGC+AGG 0.480603 6.2:-88660314 None:intergenic
GGTCATCATCCACCAAACTT+TGG 0.481752 6.2:-88659215 None:intergenic
CTTGTTTCAAAAGATAAGAT+AGG 0.487809 6.2:+88659237 MS.gene030311:CDS
AAATAACTTCCCTCAGGCTA+AGG 0.509363 6.2:+88660682 MS.gene030311:CDS
CCTCACATTGCTCGACAAGT+TGG 0.509517 6.2:-88659975 None:intergenic
TAATCAGCTACTGCTTTGGA+TGG 0.511426 6.2:-88661433 None:intergenic
TGAATTTGACTATTTGGAGA+TGG 0.520246 6.2:+88661402 MS.gene030311:CDS
TGAGAAGATGGGCCCCAAGA+AGG 0.520689 6.2:+88659161 MS.gene030311:CDS
AGATGTTGTCCAAAGTTTGG+TGG 0.534526 6.2:+88659206 MS.gene030311:CDS
AAAAGATAAGATAGGTACTT+CGG 0.536894 6.2:+88659245 MS.gene030311:CDS
TTCTTAACTCATTGCTTCAA+AGG 0.538990 6.2:-88660321 None:intergenic
CAATGTGAGGCACTAAAGAA+GGG 0.540520 6.2:+88659988 MS.gene030311:CDS
CTTAAGGAGCTTGAGAAGAT+GGG 0.546225 6.2:+88659150 MS.gene030311:CDS
GAGAAGATGGGCCCCAAGAA+GGG 0.551371 6.2:+88659162 MS.gene030311:CDS
ACAGCAGCTAACAGATGGAC+AGG 0.552808 6.2:+88660478 MS.gene030311:CDS
CTCATACAGCAGCTAACAGA+TGG 0.553951 6.2:+88660473 MS.gene030311:CDS
GAAGTTGTTCCTTAGCCTGA+GGG 0.555081 6.2:-88660691 None:intergenic
TGGAGTCTTCCTAGCTGCGC+TGG 0.556716 6.2:+88659748 MS.gene030311:CDS
GTGTTCAAATAACTTCCCTC+AGG 0.572514 6.2:+88660676 MS.gene030311:CDS
GAAGAAAAGAGGACTTTCGT+TGG 0.576601 6.2:+88657504 MS.gene030311:CDS
GCTACTGCTTTGGATGGAAG+AGG 0.578314 6.2:-88661427 None:intergenic
TTTCTAGGATGAACGAGAAG+CGG 0.584116 6.2:+88660125 MS.gene030311:intron
GACTAATCACACCCTTCTTG+GGG 0.593541 6.2:-88659173 None:intergenic
TGAAGTTGTTCCTTAGCCTG+AGG 0.605758 6.2:-88660692 None:intergenic
ACCTGATTTCCAGCGCAGCT+AGG 0.611658 6.2:-88659757 None:intergenic
AACTGCAAAACAGTGGTACA+CGG 0.615147 6.2:+88659947 MS.gene030311:CDS
GGCACTAAAGAAGGGGCGTG+AGG 0.644601 6.2:+88659996 MS.gene030311:CDS
ACAACTTCAAAACATGTACC+AGG 0.647432 6.2:+88660706 MS.gene030311:CDS
AATGTGAGGCACTAAAGAAG+GGG 0.670205 6.2:+88659989 MS.gene030311:CDS
CCAACTTGTCGAGCAATGTG+AGG 0.684357 6.2:+88659975 MS.gene030311:CDS
GATTCTGAACTGCAAAACAG+TGG 0.686674 6.2:+88659940 MS.gene030311:CDS
ACTTCAAAACATGTACCAGG+AGG 0.734825 6.2:+88660709 MS.gene030311:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAACTATACAGATAAAA+TGG - chr6.2:88660361-88660380 None:intergenic 15.0%
!! AAATTTATGAACATTGATAA+TGG - chr6.2:88657597-88657616 None:intergenic 15.0%
!! AATAAAGCATACAAAAATTT+AGG - chr6.2:88659079-88659098 None:intergenic 15.0%
!! ATAGGTTATATTTATAGAAA+AGG - chr6.2:88658594-88658613 None:intergenic 15.0%
!! TCAAATAAACCAATTTAAAT+AGG + chr6.2:88659633-88659652 MS.gene030311:intron 15.0%
!!! ATATTCTTATCTTTTTTATG+TGG + chr6.2:88660612-88660631 MS.gene030311:intron 15.0%
!!! TTTTTTATGTGGATTAAAAA+TGG + chr6.2:88660623-88660642 MS.gene030311:intron 15.0%
!! AAGTTATATCATTCCATTAA+AGG - chr6.2:88660856-88660875 None:intergenic 20.0%
!! AATAAGATGTTTATCTAAAC+AGG + chr6.2:88658667-88658686 MS.gene030311:intron 20.0%
!! ACTAATAAGGATAAAATTGA+TGG - chr6.2:88658931-88658950 None:intergenic 20.0%
!! ATTTCTATAGAAATAGCTTA+AGG - chr6.2:88658694-88658713 None:intergenic 20.0%
!! TAGAAAAAAAATGCAATGTT+AGG - chr6.2:88660111-88660130 None:intergenic 20.0%
!! TGGGTGATAATATTAATAAA+AGG - chr6.2:88661283-88661302 None:intergenic 20.0%
!! TTTCTATAGAAATAGCTTAA+GGG - chr6.2:88658693-88658712 None:intergenic 20.0%
!!! AATGTTGTTTTTGTTTAGTT+AGG + chr6.2:88657636-88657655 MS.gene030311:intron 20.0%
!!! ACGTTAAATTCTTTTTGTAT+AGG - chr6.2:88658563-88658582 None:intergenic 20.0%
!!! AGGTTAAATTTTGAGTTTAT+GGG + chr6.2:88658216-88658235 MS.gene030311:intron 20.0%
!!! ATGTTGTTTTTGTTTAGTTA+AGG + chr6.2:88657637-88657656 MS.gene030311:intron 20.0%
!!! CTGTTTTTGTATATGTTATA+AGG + chr6.2:88658726-88658745 MS.gene030311:intron 20.0%
!!! GTAGTGTTATATTTTGAAAT+AGG - chr6.2:88660987-88661006 None:intergenic 20.0%
!!! TAACATTGCATTTTTTTTCT+AGG + chr6.2:88660110-88660129 MS.gene030311:intron 20.0%
!!! TGATGTATTGGTATTAATTT+TGG + chr6.2:88660429-88660448 MS.gene030311:intron 20.0%
!!! TTATGAATGAGATATTTTGA+CGG + chr6.2:88661099-88661118 MS.gene030311:intron 20.0%
!!! TTTTTCAATCAAGACTAATA+AGG - chr6.2:88658944-88658963 None:intergenic 20.0%
! AAAAGATAAGATAGGTACTT+CGG + chr6.2:88659245-88659264 MS.gene030311:CDS 25.0%
! AAAAGTCATAACATGTAACA+TGG - chr6.2:88659382-88659401 None:intergenic 25.0%
! AACAGATGAATTTGACTATT+TGG + chr6.2:88661396-88661415 MS.gene030311:CDS 25.0%
! AAGAAAAACAGTTACTAAAC+AGG - chr6.2:88658282-88658301 None:intergenic 25.0%
! AATCGCTTTAACTCATTATT+TGG + chr6.2:88657372-88657391 MS.gene030311:intron 25.0%
! ATAGAAATGAATTGACATGA+AGG - chr6.2:88658989-88659008 None:intergenic 25.0%
! ATGAGTTAAAGCGATTTAAT+CGG - chr6.2:88657368-88657387 None:intergenic 25.0%
! ATGATGTAATTAGGTTACAT+TGG - chr6.2:88659878-88659897 None:intergenic 25.0%
! ATGATTGAATTTGTAGTGTT+TGG + chr6.2:88657817-88657836 MS.gene030311:intron 25.0%
! CTTGTTTCAAAAGATAAGAT+AGG + chr6.2:88659237-88659256 MS.gene030311:CDS 25.0%
! GCAAAATTAATAGTTGAACT+AGG + chr6.2:88657839-88657858 MS.gene030311:intron 25.0%
! GTAGTTCATAACTAGTTATT+AGG + chr6.2:88660774-88660793 MS.gene030311:intron 25.0%
! TAACTATACAGATAAAATGG+AGG - chr6.2:88660358-88660377 None:intergenic 25.0%
! TAATAAAAGGACATGTAATG+TGG - chr6.2:88661270-88661289 None:intergenic 25.0%
! TAGTCCTTTAAATTGAACAT+CGG + chr6.2:88660952-88660971 MS.gene030311:intron 25.0%
! TGATGTAATTAGGTTACATT+GGG - chr6.2:88659877-88659896 None:intergenic 25.0%
! TGTTACAAAACTCACAATAA+AGG - chr6.2:88660239-88660258 None:intergenic 25.0%
! TTGTGTTCGTTAAAAATCAA+TGG + chr6.2:88657774-88657793 MS.gene030311:intron 25.0%
!! ACTAGTTATTAGGCTTATTT+TGG + chr6.2:88660784-88660803 MS.gene030311:intron 25.0%
!! ATGTTACATGTTATGACTTT+TGG + chr6.2:88659381-88659400 MS.gene030311:intron 25.0%
!! ATTTTGTGAATGACCTTTAA+TGG + chr6.2:88660840-88660859 MS.gene030311:intron 25.0%
!! GTTACTAAACAGGTATATTT+TGG - chr6.2:88658272-88658291 None:intergenic 25.0%
!! TATCAAGTGAATAAGCATTT+TGG - chr6.2:88661222-88661241 None:intergenic 25.0%
!! TTGCTATTCGATAAATTTGA+GGG + chr6.2:88661164-88661183 MS.gene030311:intron 25.0%
!!! ACAAAAATTTAGGCTTTGTT+CGG - chr6.2:88659069-88659088 None:intergenic 25.0%
!!! CTATCTTATCTTTTGAAACA+AGG - chr6.2:88659239-88659258 None:intergenic 25.0%
!!! GAGGTTAAATTTTGAGTTTA+TGG + chr6.2:88658215-88658234 MS.gene030311:intron 25.0%
!!! GGTTAAATTTTGAGTTTATG+GGG + chr6.2:88658217-88658236 MS.gene030311:intron 25.0%
!!! GTCATTTTCATACTATATGA+TGG + chr6.2:88660583-88660602 MS.gene030311:intron 25.0%
!!! GTGTTGTTTTTGTTTAGTTA+AGG + chr6.2:88657897-88657916 MS.gene030311:intron 25.0%
!!! GTTAAATTTTGAGTTTATGG+GGG + chr6.2:88658218-88658237 MS.gene030311:intron 25.0%
!!! TATGCTTATTTTAATGATGC+CGG + chr6.2:88661025-88661044 MS.gene030311:intron 25.0%
!!! TTTGCTATTCGATAAATTTG+AGG + chr6.2:88661163-88661182 MS.gene030311:intron 25.0%
!!! TTTTTGTATAGGCTCTAAAT+TGG - chr6.2:88658552-88658571 None:intergenic 25.0%
AACTAGTTATGAACTACACA+CGG - chr6.2:88660771-88660790 None:intergenic 30.0%
ATGATATGCGCTTATGTATA+AGG - chr6.2:88658621-88658640 None:intergenic 30.0%
ATTTGATTCCTCAAAAAGCA+GGG - chr6.2:88660064-88660083 None:intergenic 30.0%
CTCAAATAAGCAATTCAAAC+AGG + chr6.2:88658860-88658879 MS.gene030311:intron 30.0%
CTTATTCACTTGATACAACT+TGG + chr6.2:88661227-88661246 MS.gene030311:intron 30.0%
GATCAATGCTTACAATTTGA+AGG - chr6.2:88660899-88660918 None:intergenic 30.0%
GTGTTTGCAATGATGTAATT+AGG - chr6.2:88659887-88659906 None:intergenic 30.0%
TAAACACTTGCGATATTGTT+TGG - chr6.2:88659598-88659617 None:intergenic 30.0%
TAAAGATGTTGTCCAAAGTT+TGG + chr6.2:88659203-88659222 MS.gene030311:CDS 30.0%
TATTGAAGTTAGCGATTCAA+AGG - chr6.2:88657324-88657343 None:intergenic 30.0%
TGAATTTGACTATTTGGAGA+TGG + chr6.2:88661402-88661421 MS.gene030311:CDS 30.0%
TTACATTGGGATACAATAGA+GGG - chr6.2:88659864-88659883 None:intergenic 30.0%
TTCTGACATAGTTACATCAA+TGG + chr6.2:88660807-88660826 MS.gene030311:intron 30.0%
TTCTTAACTCATTGCTTCAA+AGG - chr6.2:88660324-88660343 None:intergenic 30.0%
TTTGGTCCTCAAAATTGTAA+AGG - chr6.2:88661204-88661223 None:intergenic 30.0%
TTTGTCTGAATACTGTCATT+GGG + chr6.2:88660395-88660414 MS.gene030311:intron 30.0%
TTTGTTAGTGTACCATCTTT+AGG + chr6.2:88659807-88659826 MS.gene030311:intron 30.0%
! AATGGCTTTTAAATCTGCTA+GGG - chr6.2:88660151-88660170 None:intergenic 30.0%
! CGAAAAATGCTAAGATTTTC+TGG + chr6.2:88659668-88659687 MS.gene030311:intron 30.0%
! GACTGAGACTCTTTAATTTA+AGG + chr6.2:88661134-88661153 MS.gene030311:intron 30.0%
! GTTCTTCAATCTAGCTTATA+AGG + chr6.2:88658526-88658545 MS.gene030311:intron 30.0%
! TAGCATTTTTCGTTTTGCAT+TGG - chr6.2:88659660-88659679 None:intergenic 30.0%
! TATCATGTATAAGCTGACTT+AGG + chr6.2:88658383-88658402 MS.gene030311:intron 30.0%
! TGCATTGGTCCTATTTAAAT+TGG - chr6.2:88659645-88659664 None:intergenic 30.0%
! TTTTGTCTGAATACTGTCAT+TGG + chr6.2:88660394-88660413 MS.gene030311:intron 30.0%
!! ATTGGTATTAATTTTGGCAG+AGG + chr6.2:88660435-88660454 MS.gene030311:intron 30.0%
!!! ATATTTTCTCTCTGAAGCAA+TGG + chr6.2:88660654-88660673 MS.gene030311:CDS 30.0%
!!! TTTTGCTGATTTTCAGCTTA+AGG + chr6.2:88659134-88659153 MS.gene030311:intron 30.0%
AAACGCGCGTTAAAAATGAT+CGG - chr6.2:88657285-88657304 None:intergenic 35.0%
ACAACTTCAAAACATGTACC+AGG + chr6.2:88660706-88660725 MS.gene030311:CDS 35.0%
ACAGAATCCATAAACCGATT+CGG - chr6.2:88657428-88657447 None:intergenic 35.0%
ACTAGTTATGAACTACACAC+GGG - chr6.2:88660770-88660789 None:intergenic 35.0%
ATAAGCTATGAGCTCAAACA+CGG - chr6.2:88658900-88658919 None:intergenic 35.0%
ATCACAGATAGCTATGCTTT+GGG - chr6.2:88661302-88661321 None:intergenic 35.0%
ATCTGTTATACCAATCTGCA+TGG - chr6.2:88661383-88661402 None:intergenic 35.0%
CAAATTTAACCTCACCAAAC+AGG - chr6.2:88657664-88657683 None:intergenic 35.0%
CATTTGATTCCTCAAAAAGC+AGG - chr6.2:88660065-88660084 None:intergenic 35.0%
CGAATCACAAGATTTCTTTC+TGG + chr6.2:88657561-88657580 MS.gene030311:CDS 35.0%
CTTGTATTATTCTCTCTGTC+TGG + chr6.2:88659306-88659325 MS.gene030311:intron 35.0%
GAAATCAGGTGAACTTCTAT+TGG + chr6.2:88659770-88659789 MS.gene030311:intron 35.0%
GAACTATAAACAAGCATCAG+CGG - chr6.2:88659287-88659306 None:intergenic 35.0%
GCTTATGTATAAGGTTGCAT+AGG - chr6.2:88658612-88658631 None:intergenic 35.0%
GTCCTCAAAATTGTAAAGGT+CGG - chr6.2:88661200-88661219 None:intergenic 35.0%
GTTACATTGGGATACAATAG+AGG - chr6.2:88659865-88659884 None:intergenic 35.0%
GTTGTTCATTCATCCAAACA+AGG - chr6.2:88659407-88659426 None:intergenic 35.0%
TACAAGCTAGTTGCTATGTT+TGG + chr6.2:88658324-88658343 MS.gene030311:intron 35.0%
TAGAGGTTGAGACTATTTAG+AGG + chr6.2:88658096-88658115 MS.gene030311:intron 35.0%
TAGGAGCAATGATCAATTAG+AGG - chr6.2:88659705-88659724 None:intergenic 35.0%
TATAAGCTGCAAACCACTTA+TGG + chr6.2:88659555-88659574 MS.gene030311:intron 35.0%
TATCACAGATAGCTATGCTT+TGG - chr6.2:88661303-88661322 None:intergenic 35.0%
TCAAATAAGCCAATCCAAAC+AGG - chr6.2:88658410-88658429 None:intergenic 35.0%
TCGACCGATGTTCAATTTAA+AGG - chr6.2:88660959-88660978 None:intergenic 35.0%
TGTTTAGTTAGGATCCTGTT+TGG + chr6.2:88657647-88657666 MS.gene030311:intron 35.0%
TTGAGCTTATCAACTGACAT+AGG + chr6.2:88658426-88658445 MS.gene030311:intron 35.0%
TTGATTCAGTCGAAGAAAAG+AGG + chr6.2:88657493-88657512 MS.gene030311:intron 35.0%
! AAGCATAATGAGTTGAAGGT+TGG + chr6.2:88660177-88660196 MS.gene030311:intron 35.0%
! AGTTAAGGTTCTGATTGTTG+AGG + chr6.2:88658196-88658215 MS.gene030311:intron 35.0%
! CTGTTTGGTGAGGTTAAATT+TGG + chr6.2:88657662-88657681 MS.gene030311:intron 35.0%
! GAATGGCTTTTAAATCTGCT+AGG - chr6.2:88660152-88660171 None:intergenic 35.0%
! GGTGTTTTCATAAGCTATCT+TGG + chr6.2:88658747-88658766 MS.gene030311:intron 35.0%
! TACCGACCTTTACAATTTTG+AGG + chr6.2:88661195-88661214 MS.gene030311:intron 35.0%
!! ATAGGTTTTGTGAGACTGTT+TGG + chr6.2:88658444-88658463 MS.gene030311:intron 35.0%
!! CAAACACGGCATTTTGATAA+GGG - chr6.2:88658886-88658905 None:intergenic 35.0%
!! CAGAAGTGTTAGTGATATAG+AGG + chr6.2:88658079-88658098 MS.gene030311:intron 35.0%
!! TAGAAACTAGCGGATTGAAT+TGG - chr6.2:88659046-88659065 None:intergenic 35.0%
!! TAGGTTTTGTGAGACTGTTT+GGG + chr6.2:88658445-88658464 MS.gene030311:intron 35.0%
!! TCAAACACGGCATTTTGATA+AGG - chr6.2:88658887-88658906 None:intergenic 35.0%
!! TTTTTGAGGAATCAAATGGC+TGG + chr6.2:88660067-88660086 MS.gene030311:intron 35.0%
!!! CTGCTTTTTGAGGAATCAAA+TGG + chr6.2:88660063-88660082 MS.gene030311:intron 35.0%
!!! GAATGAGATATTTTGACGGA+CGG + chr6.2:88661103-88661122 MS.gene030311:intron 35.0%
!!! TTATGACTTTTGGCCTTGTT+TGG + chr6.2:88659391-88659410 MS.gene030311:intron 35.0%
AAATAACTTCCCTCAGGCTA+AGG + chr6.2:88660682-88660701 MS.gene030311:CDS 40.0%
AACTGCAAAACAGTGGTACA+CGG + chr6.2:88659947-88659966 MS.gene030311:CDS 40.0%
ACTTCAAAACATGTACCAGG+AGG + chr6.2:88660709-88660728 MS.gene030311:CDS 40.0%
AGATGTTGTCCAAAGTTTGG+TGG + chr6.2:88659206-88659225 MS.gene030311:CDS 40.0%
AGCTTAATCGAGATCCGAAT+CGG + chr6.2:88657411-88657430 MS.gene030311:intron 40.0%
ATGATCGGAAGAGAAGAAGA+AGG - chr6.2:88657270-88657289 None:intergenic 40.0%
ATGTTTCTCACCATGCAGAT+TGG + chr6.2:88661370-88661389 MS.gene030311:intron 40.0%
CCAACGTATCAACCTAAAGA+TGG - chr6.2:88659822-88659841 None:intergenic 40.0%
CCTCAAAAAGCAGGGAAAAT+TGG - chr6.2:88660056-88660075 None:intergenic 40.0%
CTAGTTATGAACTACACACG+GGG - chr6.2:88660769-88660788 None:intergenic 40.0%
CTCATTATGCTTCAGCTGAA+TGG - chr6.2:88660169-88660188 None:intergenic 40.0%
CTTAAGGAGCTTGAGAAGAT+GGG + chr6.2:88659150-88659169 MS.gene030311:CDS 40.0%
CTTTGTTCGGTAGAAACTAG+CGG - chr6.2:88659056-88659075 None:intergenic 40.0%
GAAGAAAAGAGGACTTTCGT+TGG + chr6.2:88657504-88657523 MS.gene030311:CDS 40.0%
GAATCGTGACACTGATGTAT+TGG + chr6.2:88660417-88660436 MS.gene030311:intron 40.0%
GATTCTGAACTGCAAAACAG+TGG + chr6.2:88659940-88659959 MS.gene030311:CDS 40.0%
GTGTTCAAATAACTTCCCTC+AGG + chr6.2:88660676-88660695 MS.gene030311:CDS 40.0%
TATACCTGAGAGGCAAACAT+AGG - chr6.2:88659724-88659743 None:intergenic 40.0%
TCGCATGTGTTAATGTGTGT+CGG + chr6.2:88658023-88658042 MS.gene030311:intron 40.0%
TCGGTATGCATGTCTTTCAT+TGG + chr6.2:88661063-88661082 MS.gene030311:intron 40.0%
TGACTAATCACACCCTTCTT+GGG - chr6.2:88659177-88659196 None:intergenic 40.0%
TTGTTCCCTGTATTGTGTCT+AGG + chr6.2:88660530-88660549 MS.gene030311:intron 40.0%
! AATGTGAGGCACTAAAGAAG+GGG + chr6.2:88659989-88660008 MS.gene030311:CDS 40.0%
! CAATGTGAGGCACTAAAGAA+GGG + chr6.2:88659988-88660007 MS.gene030311:CDS 40.0%
! GCTGAAGCATAATGAGTTGA+AGG + chr6.2:88660173-88660192 MS.gene030311:CDS 40.0%
! TAATCAGCTACTGCTTTGGA+TGG - chr6.2:88661436-88661455 None:intergenic 40.0%
!! CCATCTTTAGGTTGATACGT+TGG + chr6.2:88659819-88659838 MS.gene030311:intron 40.0%
!! TTTCTAGGATGAACGAGAAG+CGG + chr6.2:88660125-88660144 MS.gene030311:intron 40.0%
!!! CCAATTTTCCCTGCTTTTTG+AGG + chr6.2:88660053-88660072 MS.gene030311:intron 40.0%
!!! GCTTATTTTAATGATGCCGG+TGG + chr6.2:88661028-88661047 MS.gene030311:intron 40.0%
AAATTGGCATCTGGTAGAGG+AGG - chr6.2:88660040-88660059 None:intergenic 45.0%
AACACTTCTGATAGAAGCCG+TGG - chr6.2:88658069-88658088 None:intergenic 45.0%
AACTGAAACGACTAACCTCC+TGG - chr6.2:88660727-88660746 None:intergenic 45.0%
AAGCAGGGAAAATTGGCATC+TGG - chr6.2:88660049-88660068 None:intergenic 45.0%
AGCTGCAAACCACTTATGGA+CGG + chr6.2:88659559-88659578 MS.gene030311:intron 45.0%
CCCTGTATTGTGTCTAGGAA+GGG + chr6.2:88660535-88660554 MS.gene030311:intron 45.0%
CCCTTCCTAGACACAATACA+GGG - chr6.2:88660538-88660557 None:intergenic 45.0%
CTCACATTGCTCGACAAGTT+GGG - chr6.2:88659977-88659996 None:intergenic 45.0%
CTCATACAGCAGCTAACAGA+TGG + chr6.2:88660473-88660492 MS.gene030311:CDS 45.0%
CTGACTAATCACACCCTTCT+TGG - chr6.2:88659178-88659197 None:intergenic 45.0%
GAAGTTGTTCCTTAGCCTGA+GGG - chr6.2:88660694-88660713 None:intergenic 45.0%
GACTAATCACACCCTTCTTG+GGG - chr6.2:88659176-88659195 None:intergenic 45.0%
GACTTAGGACCTGTTTGGAT+TGG + chr6.2:88658398-88658417 MS.gene030311:intron 45.0%
GCTTAAGGAGCTTGAGAAGA+TGG + chr6.2:88659149-88659168 MS.gene030311:intron 45.0%
GGAAAATTGGCATCTGGTAG+AGG - chr6.2:88660043-88660062 None:intergenic 45.0%
GGTCATCATCCACCAAACTT+TGG - chr6.2:88659218-88659237 None:intergenic 45.0%
TCCCTGTATTGTGTCTAGGA+AGG + chr6.2:88660534-88660553 MS.gene030311:intron 45.0%
TCCCTTCCTAGACACAATAC+AGG - chr6.2:88660539-88660558 None:intergenic 45.0%
TGAAGTTGTTCCTTAGCCTG+AGG - chr6.2:88660695-88660714 None:intergenic 45.0%
TGGGATACAATAGAGGGCAA+GGG - chr6.2:88659858-88659877 None:intergenic 45.0%
TTGCCTCTCAGGTATACTTC+TGG + chr6.2:88659728-88659747 MS.gene030311:intron 45.0%
TTGGGATACAATAGAGGGCA+AGG - chr6.2:88659859-88659878 None:intergenic 45.0%
! AAGCTGACTTAGGACCTGTT+TGG + chr6.2:88658393-88658412 MS.gene030311:intron 45.0%
! ACTCCAGAAGTATACCTGAG+AGG - chr6.2:88659734-88659753 None:intergenic 45.0%
! AGTTAGGATCCTGTTTGGTG+AGG + chr6.2:88657652-88657671 MS.gene030311:intron 45.0%
! GCAATGTGAGGCACTAAAGA+AGG + chr6.2:88659987-88660006 MS.gene030311:CDS 45.0%
! GTGTTAATGTGTGTCGGTGT+CGG + chr6.2:88658029-88658048 MS.gene030311:intron 45.0%
! TCGAGATCCGAATCGGTTTA+TGG + chr6.2:88657418-88657437 MS.gene030311:intron 45.0%
!!! ATTTTTTTTTATGTAAATTA+TGG + chr6.2:88657751-88657770 MS.gene030311:intron 5.0%
ACAGCAGCTAACAGATGGAC+AGG + chr6.2:88660478-88660497 MS.gene030311:CDS 50.0%
CCAACTTGTCGAGCAATGTG+AGG + chr6.2:88659975-88659994 MS.gene030311:CDS 50.0%
CCGACTTCAATAGTCACCAC+CGG - chr6.2:88661047-88661066 None:intergenic 50.0%
CCGGTGGTGACTATTGAAGT+CGG + chr6.2:88661044-88661063 MS.gene030311:intron 50.0%
CCTCACATTGCTCGACAAGT+TGG - chr6.2:88659978-88659997 None:intergenic 50.0%
CTCATTGCTTCAAAGGCAGC+AGG - chr6.2:88660317-88660336 None:intergenic 50.0%
CTTCTGATAGAAGCCGTGGT+CGG - chr6.2:88658065-88658084 None:intergenic 50.0%
GCAAACACGATGTGCAGCTT+CGG + chr6.2:88659899-88659918 MS.gene030311:intron 50.0%
GCTGCAAACCACTTATGGAC+GGG + chr6.2:88659560-88659579 MS.gene030311:intron 50.0%
GCTTATGACCCGTCCATAAG+TGG - chr6.2:88659571-88659590 None:intergenic 50.0%
TCCTGTGTTAGTGTCTGCGT+CGG - chr6.2:88657933-88657952 None:intergenic 50.0%
TGCTCCTATGTTTGCCTCTC+AGG + chr6.2:88659717-88659736 MS.gene030311:intron 50.0%
TTGTGTCTAGGAAGGGAGAG+TGG + chr6.2:88660542-88660561 MS.gene030311:intron 50.0%
! GCTACTGCTTTGGATGGAAG+AGG - chr6.2:88661430-88661449 None:intergenic 50.0%
ACCGACGCAGACACTAACAC+AGG + chr6.2:88657929-88657948 MS.gene030311:intron 55.0%
ACCTGATTTCCAGCGCAGCT+AGG - chr6.2:88659760-88659779 None:intergenic 55.0%
CACACGGGGATTGCAGATTG+AGG - chr6.2:88660755-88660774 None:intergenic 55.0%
GAGAAGATGGGCCCCAAGAA+GGG + chr6.2:88659162-88659181 MS.gene030311:CDS 55.0%
TCCTAGCTGCGCTGGAAATC+AGG + chr6.2:88659756-88659775 MS.gene030311:CDS 55.0%
TGAGAAGATGGGCCCCAAGA+AGG + chr6.2:88659161-88659180 MS.gene030311:CDS 55.0%
! TAGAAGCCGTGGTCGGTGTT+CGG - chr6.2:88658058-88658077 None:intergenic 55.0%
TGGAGTCTTCCTAGCTGCGC+TGG + chr6.2:88659748-88659767 MS.gene030311:CDS 60.0%
! GGCACTAAAGAAGGGGCGTG+AGG + chr6.2:88659996-88660015 MS.gene030311:CDS 60.0%
CGGACACCGAACACCGACCA+CGG + chr6.2:88658049-88658068 MS.gene030311:intron 65.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 88657260 88661456 88657260 ID=MS.gene030311
chr6.2 mRNA 88657260 88661456 88657260 ID=MS.gene030311.t1;Parent=MS.gene030311
chr6.2 exon 88657260 88657262 88657260 ID=MS.gene030311.t1.exon1;Parent=MS.gene030311.t1
chr6.2 CDS 88657260 88657262 88657260 ID=cds.MS.gene030311.t1;Parent=MS.gene030311.t1
chr6.2 exon 88657502 88657582 88657502 ID=MS.gene030311.t1.exon2;Parent=MS.gene030311.t1
chr6.2 CDS 88657502 88657582 88657502 ID=cds.MS.gene030311.t1;Parent=MS.gene030311.t1
chr6.2 exon 88659150 88659266 88659150 ID=MS.gene030311.t1.exon3;Parent=MS.gene030311.t1
chr6.2 CDS 88659150 88659266 88659150 ID=cds.MS.gene030311.t1;Parent=MS.gene030311.t1
chr6.2 exon 88659739 88659777 88659739 ID=MS.gene030311.t1.exon4;Parent=MS.gene030311.t1
chr6.2 CDS 88659739 88659777 88659739 ID=cds.MS.gene030311.t1;Parent=MS.gene030311.t1
chr6.2 exon 88659916 88660023 88659916 ID=MS.gene030311.t1.exon5;Parent=MS.gene030311.t1
chr6.2 CDS 88659916 88660023 88659916 ID=cds.MS.gene030311.t1;Parent=MS.gene030311.t1
chr6.2 exon 88660132 88660194 88660132 ID=MS.gene030311.t1.exon6;Parent=MS.gene030311.t1
chr6.2 CDS 88660132 88660194 88660132 ID=cds.MS.gene030311.t1;Parent=MS.gene030311.t1
chr6.2 exon 88660284 88660335 88660284 ID=MS.gene030311.t1.exon7;Parent=MS.gene030311.t1
chr6.2 CDS 88660284 88660335 88660284 ID=cds.MS.gene030311.t1;Parent=MS.gene030311.t1
chr6.2 exon 88660455 88660499 88660455 ID=MS.gene030311.t1.exon8;Parent=MS.gene030311.t1
chr6.2 CDS 88660455 88660499 88660455 ID=cds.MS.gene030311.t1;Parent=MS.gene030311.t1
chr6.2 exon 88660651 88660730 88660651 ID=MS.gene030311.t1.exon9;Parent=MS.gene030311.t1
chr6.2 CDS 88660651 88660730 88660651 ID=cds.MS.gene030311.t1;Parent=MS.gene030311.t1
chr6.2 exon 88661388 88661456 88661388 ID=MS.gene030311.t1.exon10;Parent=MS.gene030311.t1
chr6.2 CDS 88661388 88661456 88661388 ID=cds.MS.gene030311.t1;Parent=MS.gene030311.t1
Gene Sequence

>MS.gene030311

ATGGTAACCTTCTTCTTCTCTTCCGATCATTTTTAACGCGCGTTTCGATTTTCAACTCTCTCCTTTGAATCGCTAACTTCAATACTTCACACTTTTCATTTTCAACCGATTAAATCGCTTTAACTCATTATTTGGATTCTCTACAGAAATTAGCTTAATCGAGATCCGAATCGGTTTATGGATTCTGTAAATTGCGTTTCTATGAATTGAAATTTGATTGATGTGTTTTTGTTTTGATTCAGTCGAAGAAAAGAGGACTTTCGTTGGAAGAGAAGCGAGAGAAGATGCTTCAGATCTTTTACGAATCACAAGATTTCTTTCTGGTTAGTTACTTCCATTATCAATGTTCATAAATTTTGTTATTACTTTTTAGATTAATGTTGTTTTTGTTTAGTTAGGATCCTGTTTGGTGAGGTTAAATTTGGAGCTTATAGTGTTGTTTAATAAGCTAGATTAAGTAACGTGTTTGCGTTTTGAGCTTATAACTTATAATTTTTTTTTATGTAAATTATGGTTGTGTTCGTTAAAAATCAATGGATAACTGATAAGTTAGCTATATGATTGAATTTGTAGTGTTTGGCAAAATTAATAGTTGAACTAGGTTTTAAATATGAAATGACATGAAAAGATATGTTTAGTGTTGTTTTTGTTTAGTTAAGGCTATGATGCACCGACGCAGACACTAACACAGGATATAACACTGACACGTTAACATCAATAATAAATTTAAAAAATGACATAAGCATATACAAAAAGAATTTAATCGCATGTGTTAATGTGTGTCGGTGTCGGACACCGAACACCGACCACGGCTTCTATCAGAAGTGTTAGTGATATAGAGGTTGAGACTATTTAGAGGAATGTTGTTTTTTGTTATTTCATAATCAACATTTATAAATTATGTTATTACTTTTTAGATGAATGTTGTTTTTGTTTAGTTAAGGTTCTGATTGTTGAGGTTAAATTTTGAGTTTATGGGGGATAGATTATAGCTTATAAAGTCATATCACCAAAATATACCTGTTTAGTAACTGTTTTTCTTACGAGCTTATAGCATTTGTTTTTACAAGCTAGTTGCTATGTTTGGATAAACAACTTGTATGCAGCTTATAGCACAAGCGCTTATCATGTATAAGCTGACTTAGGACCTGTTTGGATTGGCTTATTTGAGCTTATCAACTGACATAGGTTTTGTGAGACTGTTTGGGATAATTTATGAAAACAGCTTATGACAATTTTCATGTTTTTTCAAACTTATTTTCATAAGTTCTTCAATCTAGCTTATAAGGCCAATTTAGAGCCTATACAAAAAGAATTTAACGTCATTATATCCTTTTCTATAAATATAACCTATGCAACCTTATACATAAGCGCATATCATGATAAGCACTTGTGCTATAAGCTGCAAATAAGATGTTTATCTAAACAGGCCCTTAAGCTATTTCTATAGAAATAAATAAATAAAACTGTTTTTGTATATGTTATAAGGTGTTTTCATAAGCTATCTTGGAGAAGTTATGAAAATAAGCTAAACAAAAACTTATGATAAATGTCATAAGCTGTTTTCACAAGTCTTATAAAACTTATGTCACTAGATAAGCTCAAATAAGCAATTCAAACAGGCCCTTATCAAAATGCCGTGTTTGAGCTCATAGCTTATTATTTTTTCCATCAATTTTATCCTTATTAGTCTTGATTGAAAAAATATAAATATTAATTAAAAAAACCTTCATGTCAATTCATTTCTATCTACTAGCTCAATTGCTAGTTTTACTACATACTGCCAATTCAATCCGCTAGTTTCTACCGAACAAAGCCTAAATTTTTGTATGCTTTATTAGCTGATTTATATGACTTTGTTGTTGCTATTGTTGTTTTGCTGATTTTCAGCTTAAGGAGCTTGAGAAGATGGGCCCCAAGAAGGGTGTGATTAGTCAGTCTGTTAAAGATGTTGTCCAAAGTTTGGTGGATGATGACCTTGTTTCAAAAGATAAGATAGGTACTTCGGTAAGTTAAGATCTCAGCCGCTGATGCTTGTTTATAGTTCTTGTATTATTCTCTCTGTCTGGATTGTTTAAGTTTATTATGAGATTACTAGTAGAGTGCTTGTTTTGTTTTTCCATGTTACATGTTATGACTTTTGGCCTTGTTTGGATGAATGAACAACTTAATCACTTATAGCATAAGTGTTTGTTGTATTAGTGATTATGTCTAAGTTATTTCTATAATAAGAGATAAAATAAAGTCTAACTGTTTTCTTATAAACTATAAGTTGTTTTATAATGAGCTTGTTGATATAAGCTGCAAACCACTTATGGACGGGTCATAAGCTTTTCCAAACAATATCGCAAGTGTTTATGCTAGTAGATAAGCTCAAATAAACCAATTTAAATAGGACCAATGCAAAACGAAAAATGCTAAGATTTTCTGGTTGAGTTTTGTCCTCTAATTGATCATTGCTCCTATGTTTGCCTCTCAGGTATACTTCTGGAGTCTTCCTAGCTGCGCTGGAAATCAGGTGAACTTCTATTGGAAACGCTGCTAAATTTTGTTAGTGTACCATCTTTAGGTTGATACGTTGGTCACTGCTAACTACCCTTGCCCTCTATTGTATCCCAATGTAACCTAATTACATCATTGCAAACACGATGTGCAGCTTCGGAATGTACATCAAAAACTTGATTCTGAACTGCAAAACAGTGGTACACGGCATGCCCAACTTGTCGAGCAATGTGAGGCACTAAAGAAGGGGCGTGAGGAATCTGTGAGTTATAATGCCTCCTCTACCAGATGCCAATTTTCCCTGCTTTTTGAGGAATCAAATGGCTGGTCATCATTCATCATATATCCTAACATTGCATTTTTTTTCTAGGATGAACGAGAAGCGGCCCTAGCAGATTTAAAAGCCATTCAGCTGAAGCATAATGAGTTGAAGGTTGGTTTACTAATGACTTGTTAGTAATGCATTATGTATGACCTTTATTGTGAGTTTTGTAACATTCAAATAATATTTGCTATTTGCAGAATGAGTTAGAAAAATATAGAGATAATGATCCTGCTGCCTTTGAAGCAATGAGTTAAGAAATGTTTCTTTTCCTCCATTTTATCTGTATAGTTATTTGATGATTTAGAATTTTTGTCTGAATACTGTCATTGGGAATCGTGACACTGATGTATTGGTATTAATTTTGGCAGAGGAAGCAATTGAAGTTGCTCATACAGCAGCTAACAGATGGACAGGTTCTATTTTCTACGTTTTCATTGTTCATCTTGTTCCCTGTATTGTGTCTAGGAAGGGAGAGTGGAGTGTCATTATGAAATTTGTCATTTTCATACTATATGATGGTGATACATATTCTTATCTTTTTTATGTGGATTAAAAATGGTGCAGATAATATTTTCTCTCTGAAGCAATGGTGTTCAAATAACTTCCCTCAGGCTAAGGAACAACTTCAAAACATGTACCAGGAGGTTAGTCGTTTCAGTTTAATGCCTCAATCTGCAATCCCCGTGTGTAGTTCATAACTAGTTATTAGGCTTATTTTGGTTCTGACATAGTTACATCAATGGAATGATATATATTTTGTGAATGACCTTTAATGGAATGATATAACTTGAGTAAATCAGTATTTTAGTCCTTCAAATTGTAAGCATTGATCAATTTAGTTCTTGAAATTTGCGAAATAATAGTTTAGTCCTTTAAATTGAACATCGGTCGATTAGTCCTATTTCAAAATATAACACTACATTCATTTTTAAAGATTTTATGCTTATTTTAATGATGCCGGTGGTGACTATTGAAGTCGGTATGCATGTCTTTCATTGGTTTTGATAGATGTTTATGAATGAGATATTTTGACGGACGGACTAAATTGACTGAGACTCTTTAATTTAAGGCACGAATTTGCTATTCGATAAATTTGAGGGATTAAATTTACCGACCTTTACAATTTTGAGGACCAAAATGCTTATTCACTTGATACAACTTGGTTATATTATCAATATTACCACATTACATGTCCTTTTATTAATATTATCACCCAAAGCATAGCTATCTGTGATATCAGTCAAATTATCTATTCTCGTATGTCATTGTTTCTTATGTCAATCATGTTTCTCACCATGCAGATTGGTATAACAGATGAATTTGACTATTTGGAGATGGCACCTCTTCCATCCAAAGCAGTAGCTGATTAA
Protein sequence

>MS.gene030311.t1

MSKKRGLSLEEKREKMLQIFYESQDFFLLKELEKMGPKKGVISQSVKDVVQSLVDDDLVSKDKIGTSVYFWSLPSCAGNQLRNVHQKLDSELQNSGTRHAQLVEQCEALKKGREESDEREAALADLKAIQLKHNELKNELEKYRDNDPAAFEAMKEAIEVAHTAANRWTDNIFSLKQWCSNNFPQAKEQLQNMYQEIGITDEFDYLEMAPLPSKAVAD