Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030447.t1 | XP_013446994.1 | 100 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 5.80E-28 | 132.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030447.t1 | A0A072TV19 | 100.0 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 4.2e-28 | 132.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene030447 | MS.gene90025 | PPI |
| MS.gene030447 | MS.gene98091 | PPI |
| MS.gene030447 | MS.gene000435 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030447.t1 | MTR_8g096840 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 3.69e-43 | 134 |
| MS.gene030447.t1 | MTR_3g085020 | 84.058 | 69 | 11 | 0 | 1 | 69 | 1 | 69 | 2.04e-37 | 119 |
| MS.gene030447.t1 | MTR_7g028975 | 66.667 | 69 | 23 | 0 | 1 | 69 | 1 | 69 | 4.18e-33 | 108 |
| MS.gene030447.t1 | MTR_8g097020 | 62.319 | 69 | 26 | 0 | 1 | 69 | 1 | 69 | 9.24e-32 | 105 |
| MS.gene030447.t1 | MTR_8g027820 | 62.319 | 69 | 26 | 0 | 1 | 69 | 1 | 69 | 4.12e-30 | 102 |
| MS.gene030447.t1 | MTR_7g028870 | 69.697 | 66 | 20 | 0 | 2 | 67 | 3 | 68 | 4.73e-30 | 100 |
| MS.gene030447.t1 | MTR_3g085030 | 59.420 | 69 | 28 | 0 | 1 | 69 | 1 | 69 | 1.41e-28 | 97.1 |
| MS.gene030447.t1 | MTR_7g028940 | 57.971 | 69 | 29 | 0 | 1 | 69 | 1 | 69 | 2.74e-26 | 91.3 |
| MS.gene030447.t1 | MTR_8g097030 | 72.059 | 68 | 19 | 0 | 2 | 69 | 3 | 70 | 4.68e-25 | 88.2 |
| MS.gene030447.t1 | MTR_7g028815 | 60.000 | 55 | 22 | 0 | 1 | 55 | 1 | 55 | 4.37e-22 | 82.0 |
| MS.gene030447.t1 | MTR_7g028945 | 44.776 | 67 | 37 | 0 | 1 | 67 | 1 | 67 | 1.32e-16 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030447.t1 | AT3G48660 | 79.710 | 69 | 14 | 0 | 1 | 69 | 1 | 69 | 1.77e-36 | 117 |
| MS.gene030447.t1 | AT5G63500 | 81.159 | 69 | 13 | 0 | 1 | 69 | 1 | 69 | 7.26e-36 | 115 |
| MS.gene030447.t1 | AT5G08391 | 57.971 | 69 | 29 | 0 | 1 | 69 | 1 | 69 | 1.28e-29 | 99.8 |
| MS.gene030447.t1 | AT3G27027 | 68.182 | 66 | 21 | 0 | 2 | 67 | 3 | 68 | 2.10e-29 | 99.4 |
| MS.gene030447.t1 | AT3G27030 | 65.152 | 66 | 23 | 0 | 1 | 66 | 1 | 66 | 3.48e-27 | 95.9 |
| MS.gene030447.t1 | AT3G27030 | 55.072 | 69 | 31 | 0 | 1 | 69 | 74 | 142 | 1.05e-24 | 89.7 |
| MS.gene030447.t1 | AT5G40980 | 76.364 | 55 | 13 | 0 | 2 | 56 | 3 | 57 | 1.87e-26 | 92.0 |
| MS.gene030447.t1 | AT3G01950 | 55.224 | 67 | 30 | 0 | 3 | 69 | 4 | 70 | 3.09e-25 | 89.0 |
| MS.gene030447.t1 | AT5G14110 | 55.385 | 65 | 29 | 0 | 3 | 67 | 4 | 68 | 1.79e-24 | 87.0 |
| MS.gene030447.t1 | AT3G01940 | 69.697 | 66 | 20 | 0 | 2 | 67 | 4 | 69 | 1.52e-22 | 82.0 |
| MS.gene030447.t1 | AT5G50560 | 56.452 | 62 | 27 | 0 | 1 | 62 | 1 | 62 | 1.96e-17 | 68.9 |
| MS.gene030447.t1 | AT5G50660 | 56.452 | 62 | 27 | 0 | 1 | 62 | 1 | 62 | 1.96e-17 | 68.9 |
| MS.gene030447.t1 | AT5G40960 | 43.284 | 67 | 38 | 0 | 1 | 67 | 1 | 67 | 1.12e-13 | 59.7 |
Find 21 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGGAAAGGTGATTGAGTT+TGG | 0.235837 | 4.4:+6734513 | MS.gene030447:CDS |
| GGTTCAGAAAATGGCTGATT+GGG | 0.345844 | 4.4:+6734422 | None:intergenic |
| GTTCATGCCATCATTTACTT+TGG | 0.401366 | 4.4:+6734567 | MS.gene030447:CDS |
| CTGTTTGTGCTGTTGAGTCC+AGG | 0.415511 | 4.4:+6734468 | MS.gene030447:CDS |
| GAGGAAAGGTGATTGAGTTT+GGG | 0.423686 | 4.4:+6734514 | MS.gene030447:CDS |
| AGGTTCAGAAAATGGCTGAT+TGG | 0.461910 | 4.4:+6734421 | None:intergenic |
| AATGATGGCATGAACAAGAA+TGG | 0.504014 | 4.4:-6734559 | None:intergenic |
| GTTGGTGTTCACATCTACAC+TGG | 0.516469 | 4.4:+6734615 | MS.gene030447:CDS |
| GGTCTGTTGTTTCAAATACC+TGG | 0.526561 | 4.4:+6734489 | MS.gene030447:CDS |
| GAAAATGGCTGATTGGGGTC+CGG | 0.535420 | 4.4:+6734428 | None:intergenic |
| GATGAGACCAAAGTAAATGA+TGG | 0.540707 | 4.4:-6734574 | None:intergenic |
| AGCACCACCGCTATGATCAC+CGG | 0.561933 | 4.4:-6734447 | None:intergenic |
| TTGTTTCAAATACCTGGTCG+AGG | 0.567720 | 4.4:+6734495 | MS.gene030447:CDS |
| TCAAATACCTGGTCGAGGAA+AGG | 0.601837 | 4.4:+6734500 | MS.gene030447:CDS |
| TCAATCACCTTTCCTCGACC+AGG | 0.609047 | 4.4:-6734507 | None:intergenic |
| TTTGGGAACATGCAAACAAG+TGG | 0.617142 | 4.4:+6734531 | MS.gene030447:CDS |
| GTTCAGAAAATGGCTGATTG+GGG | 0.636184 | 4.4:+6734423 | None:intergenic |
| GGTATTTGAAACAACAGACC+TGG | 0.644437 | 4.4:-6734486 | None:intergenic |
| TTGGTGTTCACATCTACACT+GGG | 0.644940 | 4.4:+6734616 | MS.gene030447:CDS |
| GGGTCCGGTGATCATAGCGG+TGG | 0.646011 | 4.4:+6734443 | MS.gene030447:CDS |
| TTGGGGTCCGGTGATCATAG+CGG | 0.686200 | 4.4:+6734440 | MS.gene030447:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTATTTTACTCATTGCTGT+TGG | + | chr4.4:6734597-6734616 | MS.gene030447:CDS | 30.0% |
| AATGATGGCATGAACAAGAA+TGG | - | chr4.4:6734562-6734581 | None:intergenic | 35.0% | |
| GATGAGACCAAAGTAAATGA+TGG | - | chr4.4:6734577-6734596 | None:intergenic | 35.0% | |
| GTTCATGCCATCATTTACTT+TGG | + | chr4.4:6734567-6734586 | MS.gene030447:CDS | 35.0% | |
| GAGGAAAGGTGATTGAGTTT+GGG | + | chr4.4:6734514-6734533 | MS.gene030447:CDS | 40.0% | |
| TTGTTTCAAATACCTGGTCG+AGG | + | chr4.4:6734495-6734514 | MS.gene030447:CDS | 40.0% | |
| TTTGGGAACATGCAAACAAG+TGG | + | chr4.4:6734531-6734550 | MS.gene030447:CDS | 40.0% | |
| ! | GGTATTTGAAACAACAGACC+TGG | - | chr4.4:6734489-6734508 | None:intergenic | 40.0% |
| ! | TTGGTGTTCACATCTACACT+GGG | + | chr4.4:6734616-6734635 | MS.gene030447:CDS | 40.0% |
| !! | GGTCTGTTGTTTCAAATACC+TGG | + | chr4.4:6734489-6734508 | MS.gene030447:CDS | 40.0% |
| CGAGGAAAGGTGATTGAGTT+TGG | + | chr4.4:6734513-6734532 | MS.gene030447:CDS | 45.0% | |
| TCAAATACCTGGTCGAGGAA+AGG | + | chr4.4:6734500-6734519 | MS.gene030447:CDS | 45.0% | |
| ! | GTTGGTGTTCACATCTACAC+TGG | + | chr4.4:6734615-6734634 | MS.gene030447:CDS | 45.0% |
| TCAATCACCTTTCCTCGACC+AGG | - | chr4.4:6734510-6734529 | None:intergenic | 50.0% | |
| ! | CTGTTTGTGCTGTTGAGTCC+AGG | + | chr4.4:6734468-6734487 | MS.gene030447:CDS | 50.0% |
| AGCACCACCGCTATGATCAC+CGG | - | chr4.4:6734450-6734469 | None:intergenic | 55.0% | |
| TTGGGGTCCGGTGATCATAG+CGG | + | chr4.4:6734440-6734459 | MS.gene030447:CDS | 55.0% | |
| GGGTCCGGTGATCATAGCGG+TGG | + | chr4.4:6734443-6734462 | MS.gene030447:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 6734432 | 6734641 | 6734432 | ID=MS.gene030447 |
| chr4.4 | mRNA | 6734432 | 6734641 | 6734432 | ID=MS.gene030447.t1;Parent=MS.gene030447 |
| chr4.4 | exon | 6734432 | 6734641 | 6734432 | ID=MS.gene030447.t1.exon1;Parent=MS.gene030447.t1 |
| chr4.4 | CDS | 6734432 | 6734641 | 6734432 | ID=cds.MS.gene030447.t1;Parent=MS.gene030447.t1 |
| Gene Sequence |
| Protein sequence |
