AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031228

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031228.t1 MTR_3g110190 96.232 345 9 3 1 342 1 344 0.0 670
MS.gene031228.t1 MTR_6g087150 44.476 353 129 11 10 326 39 360 1.31e-80 250
MS.gene031228.t1 MTR_5g066250 52.239 134 58 3 10 142 82 210 3.79e-36 135
MS.gene031228.t1 MTR_3g100400 47.586 145 57 2 10 142 69 206 1.44e-34 131
MS.gene031228.t1 MTR_1g082550 41.538 130 68 3 8 134 45 169 6.83e-30 117
MS.gene031228.t1 MTR_4g106680 43.662 142 70 3 8 140 40 180 4.31e-29 114
MS.gene031228.t1 MTR_7g113800 42.188 128 64 3 10 134 42 162 5.62e-28 112
MS.gene031228.t1 MTR_6g007737 37.908 153 83 3 8 149 67 218 1.20e-25 106
MS.gene031228.t1 MTR_3g099650 40.945 127 66 3 11 134 58 178 5.79e-25 104
MS.gene031228.t1 MTR_4g120270 52.439 82 37 1 62 143 76 155 7.13e-25 102
MS.gene031228.t1 MTR_5g064980 39.535 129 71 3 11 134 53 179 7.07e-24 101
MS.gene031228.t1 MTR_8g063660 38.636 132 66 3 15 134 8 136 1.36e-23 99.8
MS.gene031228.t1 MTR_2g090950 55.556 81 30 1 64 138 89 169 2.23e-23 97.1
MS.gene031228.t1 MTR_4g129420 45.455 132 64 4 8 134 58 186 2.80e-23 100
MS.gene031228.t1 MTR_2g090905 54.321 81 31 1 64 138 37 117 1.15e-22 92.0
MS.gene031228.t1 MTR_8g089880 53.623 69 32 0 66 134 127 195 3.81e-22 94.7
MS.gene031228.t1 MTR_2g436100 33.803 142 86 2 5 138 24 165 5.09e-22 94.7
MS.gene031228.t1 MTR_5g064990 36.641 131 75 3 15 141 67 193 1.54e-21 94.7
MS.gene031228.t1 MTR_5g034790 35.714 140 77 1 8 134 45 184 3.80e-21 93.6
MS.gene031228.t1 MTR_5g064890 43.478 92 52 0 43 134 105 196 5.48e-21 92.8
MS.gene031228.t1 MTR_6g055030 34.375 128 73 2 10 134 49 168 1.31e-20 91.3
MS.gene031228.t1 MTR_2g009310 55.072 69 31 0 66 134 135 203 1.47e-20 92.0
MS.gene031228.t1 MTR_8g023400 52.778 72 33 1 66 137 92 162 6.35e-20 89.4
MS.gene031228.t1 MTR_5g076990 34.109 129 81 1 10 134 20 148 7.08e-20 89.0
MS.gene031228.t1 MTR_5g030010 35.659 129 71 4 66 189 122 243 9.96e-20 89.4
MS.gene031228.t1 MTR_4g093830 52.174 69 33 0 66 134 126 194 1.11e-19 89.0
MS.gene031228.t1 MTR_8g009190 32.278 158 97 4 6 156 17 171 1.23e-19 88.6
MS.gene031228.t1 MTR_3g091620 42.667 75 43 0 63 137 98 172 2.73e-19 87.8
MS.gene031228.t1 MTR_3g464780 45.455 88 42 2 60 141 96 183 3.56e-19 85.9
MS.gene031228.t1 MTR_8g092840 47.436 78 40 1 64 140 111 188 5.05e-19 85.1
MS.gene031228.t1 MTR_8g092960 47.436 78 40 1 64 140 101 178 5.29e-19 84.7
MS.gene031228.t1 MTR_8g092985 44.444 81 44 1 61 140 106 186 1.02e-18 83.6
MS.gene031228.t1 MTR_2g096350 40.816 98 55 2 56 150 96 193 1.29e-18 83.6
MS.gene031228.t1 MTR_5g024730 29.530 149 94 3 23 168 33 173 1.40e-18 83.6
MS.gene031228.t1 MTR_8g066740 33.333 135 85 2 5 134 14 148 1.40e-18 85.1
MS.gene031228.t1 MTR_7g076250 40.217 92 50 2 64 150 61 152 1.68e-18 82.8
MS.gene031228.t1 MTR_4g063070 35.537 121 68 3 24 134 258 378 2.61e-18 85.5
MS.gene031228.t1 MTR_2g044570 58.730 63 23 1 75 134 63 125 4.09e-18 84.0
MS.gene031228.t1 MTR_1g492830 32.593 135 83 3 11 139 3 135 4.25e-18 82.4
MS.gene031228.t1 MTR_3g095560 35.659 129 77 3 15 140 59 184 5.39e-18 82.4
MS.gene031228.t1 MTR_8g093010 45.000 80 43 1 62 140 103 182 5.64e-18 82.0
MS.gene031228.t1 MTR_7g116220 32.624 141 81 3 14 141 9 148 9.77e-18 80.1
MS.gene031228.t1 MTR_8g073120 34.127 126 66 3 14 134 27 140 1.17e-17 80.5
MS.gene031228.t1 MTR_1g070140 35.115 131 73 5 15 134 43 172 1.20e-17 83.2
MS.gene031228.t1 MTR_7g096690 31.481 162 95 7 15 166 14 169 1.64e-17 82.4
MS.gene031228.t1 MTR_8g066780 33.728 169 94 7 14 173 58 217 1.65e-17 82.8
MS.gene031228.t1 MTR_5g071930 47.368 76 37 1 66 141 66 138 3.08e-17 79.3
MS.gene031228.t1 MTR_1g043410 34.559 136 70 6 14 134 34 165 4.05e-17 81.3
MS.gene031228.t1 MTR_1g070425 43.750 80 39 2 66 139 68 147 4.95e-17 78.2
MS.gene031228.t1 MTR_8g092930 44.737 76 41 1 66 140 112 187 4.96e-17 79.3
MS.gene031228.t1 MTR_5g092790 48.684 76 37 1 66 139 157 232 5.13e-17 79.7
MS.gene031228.t1 MTR_7g116180 43.750 80 39 2 66 139 67 146 5.40e-17 78.2
MS.gene031228.t1 MTR_4g094725 43.210 81 44 1 60 138 72 152 6.04e-17 77.4
MS.gene031228.t1 MTR_3g071300 31.707 123 75 2 16 134 31 148 1.16e-16 77.0
MS.gene031228.t1 MTR_5g092750 40.566 106 58 3 38 138 127 232 1.19e-16 78.6
MS.gene031228.t1 MTR_3g078120 32.520 123 79 2 16 134 5 127 1.42e-16 76.3
MS.gene031228.t1 MTR_5g026720 32.283 127 72 3 14 134 17 135 1.66e-16 76.3
MS.gene031228.t1 MTR_7g117540 40.157 127 73 2 8 134 20 143 1.81e-16 79.0
MS.gene031228.t1 MTR_1g074190 41.333 75 38 1 66 134 99 173 2.14e-16 79.3
MS.gene031228.t1 MTR_8g027980 42.708 96 42 4 69 159 93 180 3.17e-16 77.8
MS.gene031228.t1 MTR_3g094000 38.824 85 50 1 57 139 82 166 3.44e-16 75.9
MS.gene031228.t1 MTR_3g086700 46.377 69 34 1 69 134 49 117 3.69e-16 76.3
MS.gene031228.t1 MTR_5g026730 31.333 150 82 4 7 144 6 146 4.67e-16 75.5
MS.gene031228.t1 MTR_3g086730 46.377 69 34 1 69 134 49 117 5.34e-16 76.6
MS.gene031228.t1 MTR_3g078470 45.070 71 37 1 66 134 76 146 5.86e-16 76.3
MS.gene031228.t1 MTR_7g079280 39.437 71 41 1 66 134 80 150 7.27e-16 75.1
MS.gene031228.t1 MTR_3g078450 47.222 72 34 2 66 134 76 146 9.21e-16 75.9
MS.gene031228.t1 MTR_2g035800 40.476 84 48 1 58 141 70 151 9.98e-16 74.3
MS.gene031228.t1 MTR_4g030070 39.286 84 50 1 51 134 70 152 1.34e-15 75.5
MS.gene031228.t1 MTR_2g055790 46.377 69 34 1 66 134 77 142 1.48e-15 74.7
MS.gene031228.t1 MTR_3g116000 33.333 123 78 3 16 137 30 149 1.68e-15 74.3
MS.gene031228.t1 MTR_8g032690 45.161 62 30 1 92 149 19 80 1.90e-15 72.0
MS.gene031228.t1 MTR_1g100737 38.158 76 42 1 64 134 90 165 5.07e-15 73.2
MS.gene031228.t1 MTR_7g073200 38.462 78 48 0 57 134 72 149 6.89e-15 72.4
MS.gene031228.t1 MTR_7g116200 44.928 69 37 1 66 134 68 135 8.76e-15 72.0
MS.gene031228.t1 MTR_3g012550 31.618 136 84 3 7 134 16 150 1.01e-14 71.6
MS.gene031228.t1 MTR_4g056590 36.842 76 45 1 62 137 96 168 1.29e-14 72.0
MS.gene031228.t1 MTR_7g108855 39.759 83 46 2 67 146 67 148 1.97e-14 71.2
MS.gene031228.t1 MTR_8g092970 34.351 131 75 3 11 140 59 179 2.29e-14 71.6
MS.gene031228.t1 MTR_8g028465 37.681 69 41 1 66 134 90 156 3.05e-14 70.9
MS.gene031228.t1 MTR_8g092990 56.000 50 22 0 91 140 140 189 3.32e-14 71.6
MS.gene031228.t1 MTR_5g082940 43.750 80 41 2 58 134 67 145 4.20e-14 69.7
MS.gene031228.t1 MTR_8g019710 45.455 66 35 1 74 139 37 101 6.95e-14 68.6
MS.gene031228.t1 MTR_6g007680 28.099 121 74 2 17 137 4 111 9.98e-14 68.6
MS.gene031228.t1 MTR_7g116210 43.478 69 38 1 66 134 10 77 1.35e-13 67.0
MS.gene031228.t1 MTR_8g028815 46.032 63 33 1 73 134 73 135 1.60e-13 68.2
MS.gene031228.t1 MTR_1g056350 36.000 75 45 1 63 134 71 145 2.09e-13 67.8
MS.gene031228.t1 MTR_3g114500 41.176 85 44 2 56 134 29 113 2.17e-13 66.6
MS.gene031228.t1 MTR_4g022410 34.646 127 64 5 14 137 48 158 2.65e-13 68.2
MS.gene031228.t1 MTR_2g041200 63.636 44 16 0 91 134 108 151 2.67e-13 67.8
MS.gene031228.t1 MTR_7g059360 54.717 53 24 0 85 137 151 203 2.72e-13 68.9
MS.gene031228.t1 MTR_8g099495 51.020 49 24 0 92 140 347 395 2.85e-13 70.5
MS.gene031228.t1 MTR_5g022920 36.145 83 45 2 54 134 155 231 5.86e-13 68.2
MS.gene031228.t1 MTR_8g074900 42.254 71 37 2 64 134 156 222 9.18e-13 67.4
MS.gene031228.t1 MTR_6g055030 63.415 41 15 0 94 134 97 137 1.19e-12 67.8
MS.gene031228.t1 MTR_1g090480 35.616 73 43 1 66 134 72 144 1.29e-12 65.5
MS.gene031228.t1 MTR_3g078520 37.143 70 43 1 66 134 76 145 1.71e-12 65.1
MS.gene031228.t1 MTR_3g081030 39.080 87 46 2 54 134 76 161 2.97e-12 65.5
MS.gene031228.t1 MTR_8g071815 31.496 127 72 5 13 134 16 132 3.06e-12 64.7
MS.gene031228.t1 MTR_7g079290 32.877 73 45 1 66 134 125 197 3.34e-12 65.5
MS.gene031228.t1 MTR_1g026070 38.889 72 41 1 63 134 45 113 3.50e-12 63.5
MS.gene031228.t1 MTR_7g117350 51.163 43 21 0 92 134 322 364 7.93e-12 66.2
MS.gene031228.t1 MTR_7g117350 51.163 43 21 0 92 134 314 356 8.66e-12 65.9
MS.gene031228.t1 MTR_5g041330 37.647 85 47 3 51 135 69 147 9.23e-12 64.3
MS.gene031228.t1 MTR_5g041310 37.647 85 47 3 51 135 79 157 9.67e-12 64.3
MS.gene031228.t1 MTR_1g090450 34.646 127 69 4 18 134 27 149 1.59e-11 62.4
MS.gene031228.t1 MTR_3g087900 60.000 45 16 2 91 134 68 111 1.59e-11 62.4
MS.gene031228.t1 MTR_1g090460 34.409 93 55 2 48 134 45 137 3.57e-11 61.2
MS.gene031228.t1 MTR_6g007670 39.394 66 39 1 72 137 14 78 4.07e-11 62.8
MS.gene031228.t1 MTR_4g108830 33.645 107 59 4 38 134 112 216 4.84e-11 62.0
MS.gene031228.t1 MTR_4g108830 34.783 92 52 3 49 134 99 188 5.19e-11 61.6
MS.gene031228.t1 MTR_8g072020 40.741 81 42 4 71 146 384 463 5.80e-11 63.9
MS.gene031228.t1 MTR_8g099515 51.163 43 21 0 92 134 337 379 5.95e-11 63.5
MS.gene031228.t1 MTR_8g072020 40.741 81 42 4 71 146 423 502 6.00e-11 63.9
MS.gene031228.t1 MTR_5g009210 51.064 47 23 0 92 138 315 361 6.01e-11 63.5
MS.gene031228.t1 MTR_4g102870 47.273 55 28 1 86 140 310 363 6.08e-11 63.5
MS.gene031228.t1 MTR_5g009190 36.000 75 46 1 66 138 266 340 6.96e-11 63.2
MS.gene031228.t1 MTR_8g027745 44.231 52 28 1 83 134 306 356 9.94e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031228.t1 AT2G20030 40.290 345 171 10 10 326 39 376 3.19e-69 221
MS.gene031228.t1 AT4G28890 37.438 406 183 11 2 338 27 430 1.58e-67 218
MS.gene031228.t1 AT5G05810 32.571 350 175 11 8 298 54 401 1.19e-35 134
MS.gene031228.t1 AT4G40070 44.526 137 64 4 3 134 37 166 1.80e-31 121
MS.gene031228.t1 AT1G72200 44.697 132 60 4 10 134 61 186 2.05e-30 119
MS.gene031228.t1 AT3G05200 39.568 139 69 4 8 139 45 175 1.18e-28 114
MS.gene031228.t1 AT1G22500 41.860 129 68 3 9 134 36 160 3.17e-27 110
MS.gene031228.t1 AT2G35000 35.135 148 84 4 15 151 51 197 5.32e-26 107
MS.gene031228.t1 AT1G23980 38.406 138 74 2 8 134 49 186 2.13e-25 105
MS.gene031228.t1 AT4G17920 40.299 134 73 3 8 134 19 152 3.47e-25 103
MS.gene031228.t1 AT1G35330 38.760 129 75 2 10 134 43 171 1.92e-24 102
MS.gene031228.t1 AT5G27420 39.844 128 61 4 19 139 53 171 2.29e-24 102
MS.gene031228.t1 AT5G57750 38.129 139 78 3 3 134 27 164 4.40e-24 98.6
MS.gene031228.t1 AT5G17600 37.255 153 87 5 14 161 61 209 1.11e-23 100
MS.gene031228.t1 AT4G30400 62.500 72 26 1 64 134 105 176 1.32e-23 101
MS.gene031228.t1 AT5G46650 37.226 137 73 4 11 134 21 157 2.47e-23 98.2
MS.gene031228.t1 AT2G18650 61.111 72 27 1 64 134 97 168 5.54e-23 99.4
MS.gene031228.t1 AT4G09120 56.944 72 28 1 66 134 94 165 7.22e-23 97.8
MS.gene031228.t1 AT5G40250 35.616 146 74 3 8 134 41 185 1.50e-22 97.4
MS.gene031228.t1 AT3G16720 54.795 73 32 1 66 138 94 165 4.01e-22 95.1
MS.gene031228.t1 AT4G33565 52.632 76 36 0 66 141 191 266 9.85e-22 95.1
MS.gene031228.t1 AT4G09110 52.778 72 31 1 66 134 94 165 1.38e-21 93.6
MS.gene031228.t1 AT4G09130 40.678 118 58 3 63 177 88 196 1.89e-21 94.4
MS.gene031228.t1 AT2G34990 34.074 135 84 2 5 134 26 160 2.46e-21 93.2
MS.gene031228.t1 AT2G34990 34.074 135 84 2 5 134 4 138 2.60e-21 92.8
MS.gene031228.t1 AT2G35420 36.691 139 75 3 2 134 14 145 2.99e-21 91.7
MS.gene031228.t1 AT4G15975 46.914 81 42 1 58 138 43 122 4.01e-21 90.9
MS.gene031228.t1 AT3G48030 35.507 138 80 3 14 142 42 179 1.45e-20 90.1
MS.gene031228.t1 AT5G10380 52.778 72 34 0 66 137 109 180 1.65e-20 90.5
MS.gene031228.t1 AT3G48030 35.507 138 80 3 14 142 120 257 1.99e-20 91.3
MS.gene031228.t1 AT3G03550 48.052 77 39 1 67 142 134 210 3.20e-20 90.5
MS.gene031228.t1 AT3G18773 37.500 136 70 5 13 140 50 178 1.65e-19 86.3
MS.gene031228.t1 AT3G60220 55.844 77 29 2 71 142 93 169 3.35e-19 87.4
MS.gene031228.t1 AT4G09100 45.833 72 36 1 66 134 56 127 5.81e-19 82.4
MS.gene031228.t1 AT4G17905 32.394 142 79 3 10 134 56 197 6.43e-19 86.3
MS.gene031228.t1 AT1G53820 47.297 74 38 1 64 137 93 165 9.60e-19 85.9
MS.gene031228.t1 AT1G72220 48.571 70 36 0 65 134 150 219 9.76e-19 87.0
MS.gene031228.t1 AT4G17905 32.394 142 79 3 10 134 82 223 1.57e-18 85.5
MS.gene031228.t1 AT2G42360 33.858 127 70 2 13 138 41 154 2.36e-18 83.2
MS.gene031228.t1 AT1G04360 32.847 137 69 4 14 134 47 176 4.08e-18 84.7
MS.gene031228.t1 AT1G20823 36.923 130 72 3 14 137 31 156 4.84e-18 81.6
MS.gene031228.t1 AT5G43420 30.405 148 69 3 14 134 40 180 4.90e-18 84.7
MS.gene031228.t1 AT2G42350 46.154 78 41 1 66 143 75 151 5.86e-18 82.0
MS.gene031228.t1 AT3G10910 39.773 88 50 1 47 134 72 156 9.27e-18 80.5
MS.gene031228.t1 AT1G49220 45.556 90 43 3 54 140 97 183 9.32e-18 82.0
MS.gene031228.t1 AT1G49200 34.586 133 77 4 13 140 57 184 9.63e-18 81.3
MS.gene031228.t1 AT1G49210 46.154 78 41 1 64 140 106 183 1.01e-17 81.3
MS.gene031228.t1 AT1G76410 34.400 125 77 2 14 137 29 149 1.11e-17 80.1
MS.gene031228.t1 AT2G47560 48.052 77 38 1 64 138 78 154 1.27e-17 81.3
MS.gene031228.t1 AT2G27940 30.827 133 75 4 15 134 54 182 1.87e-17 80.9
MS.gene031228.t1 AT1G72310 48.684 76 34 2 64 137 100 172 3.79e-17 81.6
MS.gene031228.t1 AT5G01880 42.254 71 41 0 65 135 78 148 4.41e-17 77.8
MS.gene031228.t1 AT5G66160 34.752 141 72 5 10 148 161 283 6.60e-17 80.5
MS.gene031228.t1 AT1G49230 36.522 115 67 2 61 174 99 208 6.93e-17 79.0
MS.gene031228.t1 AT2G17450 42.353 85 42 2 60 137 63 147 8.34e-17 77.8
MS.gene031228.t1 AT5G66160 34.752 141 72 5 10 148 161 283 1.22e-16 79.7
MS.gene031228.t1 AT3G62690 44.595 74 41 0 67 140 88 161 1.76e-16 78.6
MS.gene031228.t1 AT1G32361 40.698 86 47 2 50 134 114 196 3.00e-16 79.0
MS.gene031228.t1 AT3G18930 41.463 82 38 1 66 137 120 201 3.16e-16 79.7
MS.gene031228.t1 AT3G18930 41.463 82 38 1 66 137 120 201 3.16e-16 79.7
MS.gene031228.t1 AT3G18930 41.463 82 38 1 66 137 120 201 3.16e-16 79.7
MS.gene031228.t1 AT3G14320 47.826 69 35 1 66 134 63 130 3.86e-16 76.6
MS.gene031228.t1 AT5G05280 31.967 122 74 3 14 134 42 155 5.63e-16 75.5
MS.gene031228.t1 AT2G46493 31.783 129 78 4 11 134 57 180 8.53e-16 75.1
MS.gene031228.t1 AT4G10150 34.677 124 71 4 18 134 33 153 8.84e-16 76.3
MS.gene031228.t1 AT3G19140 44.186 86 45 2 65 150 53 135 8.96e-16 73.9
MS.gene031228.t1 AT1G74410 41.892 74 43 0 66 139 150 223 2.16e-15 74.7
MS.gene031228.t1 AT1G28040 35.870 92 51 2 48 134 207 295 2.75e-15 75.9
MS.gene031228.t1 AT2G35910 39.130 69 42 0 66 134 121 189 6.00e-15 73.6
MS.gene031228.t1 AT1G33480 31.613 155 90 4 62 216 71 209 7.69e-15 73.9
MS.gene031228.t1 AT2G34000 30.709 127 74 2 16 138 21 137 1.05e-14 71.2
MS.gene031228.t1 AT4G35480 30.769 143 69 4 14 137 27 158 1.41e-14 72.0
MS.gene031228.t1 AT5G07040 30.882 136 77 5 10 134 7 136 1.97e-14 70.5
MS.gene031228.t1 AT4G10160 43.478 69 38 1 66 134 72 139 2.15e-14 72.0
MS.gene031228.t1 AT2G46160 36.364 77 41 1 66 134 104 180 2.49e-14 71.6
MS.gene031228.t1 AT2G46160 36.364 77 41 1 66 134 104 180 2.49e-14 71.6
MS.gene031228.t1 AT1G80400 30.556 144 64 5 21 140 271 402 5.58e-14 72.8
MS.gene031228.t1 AT3G61550 33.735 83 44 1 63 134 96 178 8.73e-14 70.1
MS.gene031228.t1 AT4G30370 38.028 71 44 0 64 134 87 157 3.47e-13 67.4
MS.gene031228.t1 AT3G20395 42.029 69 38 1 66 134 147 213 5.18e-13 68.2
MS.gene031228.t1 AT5G66070 34.314 102 54 3 44 134 119 218 6.04e-13 67.8
MS.gene031228.t1 AT5G66070 34.314 102 54 3 44 134 119 218 6.04e-13 67.8
MS.gene031228.t1 AT5G58580 30.667 150 86 5 64 195 109 258 6.07e-13 68.9
MS.gene031228.t1 AT2G46494 32.331 133 76 5 11 134 231 358 6.80e-13 69.3
MS.gene031228.t1 AT2G46495 30.075 133 67 4 16 134 242 362 7.39e-13 69.3
MS.gene031228.t1 AT5G66070 41.096 73 41 1 62 134 172 242 9.85e-13 67.4
MS.gene031228.t1 AT2G46495 36.000 75 45 1 63 134 260 334 1.11e-12 68.6
MS.gene031228.t1 AT4G11370 29.104 134 88 3 4 135 3 131 1.16e-12 65.5
MS.gene031228.t1 AT5G06490 36.842 76 41 1 66 134 96 171 1.31e-12 66.2
MS.gene031228.t1 AT2G18670 33.058 121 80 1 15 134 32 152 1.38e-12 65.9
MS.gene031228.t1 AT5G53110 31.967 122 75 3 15 134 257 372 1.41e-12 68.6
MS.gene031228.t1 AT5G47610 46.774 62 32 1 74 134 88 149 1.64e-12 65.5
MS.gene031228.t1 AT1G26800 40.625 64 32 2 72 135 98 155 1.81e-12 66.2
MS.gene031228.t1 AT3G61460 32.283 127 76 4 14 134 15 137 2.10e-12 65.1
MS.gene031228.t1 AT3G11110 28.926 121 78 2 15 134 36 149 2.71e-12 64.7
MS.gene031228.t1 AT1G53010 30.303 165 82 6 7 140 16 178 3.37e-12 64.7
MS.gene031228.t1 AT4G17245 60.000 45 17 1 91 134 101 145 3.62e-12 64.3
MS.gene031228.t1 AT2G37580 51.667 60 28 1 79 137 128 187 4.14e-12 65.5
MS.gene031228.t1 AT4G24015 50.000 60 29 1 85 144 99 157 5.82e-12 63.9
MS.gene031228.t1 AT4G35840 38.889 72 41 1 66 134 162 233 7.66e-12 64.7
MS.gene031228.t1 AT1G63170 60.000 45 17 1 91 135 324 367 1.09e-11 65.9
MS.gene031228.t1 AT4G26400 39.726 73 37 2 69 141 224 289 1.27e-11 65.5
MS.gene031228.t1 AT4G26400 39.726 73 37 2 69 141 224 289 1.27e-11 65.5
MS.gene031228.t1 AT2G25410 37.500 72 42 1 66 134 298 369 1.82e-11 65.1
MS.gene031228.t1 AT5G56340 38.202 89 47 3 69 156 242 323 2.38e-11 64.7
MS.gene031228.t1 AT2G17730 37.500 72 42 1 66 134 167 238 3.32e-11 63.2
MS.gene031228.t1 AT1G12760 36.000 100 33 3 66 135 297 395 4.70e-11 63.9
MS.gene031228.t1 AT1G12760 36.000 100 33 3 66 135 226 324 5.82e-11 63.2
MS.gene031228.t1 AT4G11360 43.750 64 33 2 73 134 67 129 8.72e-11 60.1
MS.gene031228.t1 AT3G19950 34.667 75 48 1 67 141 191 264 9.20e-11 62.8

Find 47 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATTCTCTTCTTTAAA+AGG 0.139116 3.4:-95187226 MS.gene031228:CDS
CTTGAAATTCCATGAAATTC+AGG 0.251567 3.4:+95186698 None:intergenic
TGAATAAAGATTTCAAGATT+TGG 0.257283 3.4:+95186968 None:intergenic
AGATTCTCTCCTGAATTTCA+TGG 0.283990 3.4:-95186707 MS.gene031228:CDS
TTACTTGGAGTAACACCTTT+TGG 0.325381 3.4:+95187445 None:intergenic
ATGAGAGGATGTGGAGGATT+TGG 0.333871 3.4:-95186547 MS.gene031228:CDS
GAATAAAGATTTCAAGATTT+GGG 0.337955 3.4:+95186969 None:intergenic
TTTGGGTTTAGAAGATGAAC+AGG 0.347996 3.4:+95187340 None:intergenic
GGTACATTCTAAGCCTTGTT+TGG 0.350953 3.4:+95187201 None:intergenic
ACTCTACTTGTCCTCTTTGC+AGG 0.359957 3.4:-95187078 MS.gene031228:CDS
CTTAATCTTCAAAACAATCT+AGG 0.372210 3.4:-95187313 MS.gene031228:CDS
TGAAAAGCATGCTTGCATTT+CGG 0.375773 3.4:+95187133 None:intergenic
CGATCGAGGTCTATAAATTC+TGG 0.391790 3.4:-95187283 MS.gene031228:CDS
TGAACTGTTCTCCTTGCAAT+TGG 0.394010 3.4:+95186521 None:intergenic
CTGCTGATTTCGACAAACCT+TGG 0.401436 3.4:+95186593 None:intergenic
ATTATTACAACACCTTTACT+TGG 0.404385 3.4:+95187430 None:intergenic
TTTGAAGATTAAGAGAGTTT+GGG 0.405592 3.4:+95187323 None:intergenic
GGGTCCTCGCTTAAATTTGA+AGG 0.429221 3.4:+95186989 None:intergenic
AGCAGAAGAAGCGGGGAGAA+TGG 0.451139 3.4:-95186575 MS.gene031228:CDS
AAAGAAGAGAATTTGAAGAA+AGG 0.452758 3.4:+95187232 None:intergenic
TTCAAATTTCGATAAACAAA+CGG 0.467871 3.4:+95187180 None:intergenic
CTTCTTGATCATGAAGAAAG+TGG 0.478062 3.4:-95186890 MS.gene031228:CDS
TTGCAAGGAGAACAGTTCAA+TGG 0.492911 3.4:-95186517 MS.gene031228:CDS
ATGGAAGTGATGAGAGGATG+TGG 0.493739 3.4:-95186556 MS.gene031228:CDS
TCGTTTACAGCATTGAATCC+TGG 0.495608 3.4:-95186650 MS.gene031228:CDS
TCGAAATCAGCAGAAGAAGC+GGG 0.512061 3.4:-95186583 MS.gene031228:CDS
ACATGAATTGTATTGACAAG+TGG 0.534529 3.4:-95187111 MS.gene031228:CDS
AAAGGTGTTACTCCAAGTAA+AGG 0.544695 3.4:-95187442 MS.gene031228:CDS
GGTCCTCGCTTAAATTTGAA+GGG 0.544773 3.4:+95186990 None:intergenic
CTGAGATATCAAAAGTTCCA+AGG 0.562845 3.4:-95186610 MS.gene031228:CDS
AACATGGCAGAGTTACCAAA+AGG 0.573528 3.4:-95187460 MS.gene031228:CDS
GGATTTGGATGCCAATTGCA+AGG 0.578152 3.4:-95186532 MS.gene031228:CDS
TAATCTCTGATGTTGTTACA+AGG 0.580401 3.4:-95186805 MS.gene031228:CDS
TCTTTAAAAGGATCCAAACA+AGG 0.584756 3.4:-95187214 MS.gene031228:CDS
TTATGATTAATCATATGCAA+AGG 0.586045 3.4:+95186833 None:intergenic
GTCGAAATCAGCAGAAGAAG+CGG 0.588001 3.4:-95186584 MS.gene031228:CDS
GGGAGAATGGAAGTGATGAG+AGG 0.593662 3.4:-95186562 MS.gene031228:CDS
GACATACTCCTCTTCTCACC+AGG 0.596787 3.4:+95186632 None:intergenic
CATTGAATCCTGGTGAGAAG+AGG 0.597963 3.4:-95186640 MS.gene031228:CDS
CTGATGTTGTTACAAGGAGT+AGG 0.615884 3.4:-95186799 MS.gene031228:CDS
TCAACTATGTACCTGCAAAG+AGG 0.618892 3.4:+95187067 None:intergenic
ATGTTGTTACAAGGAGTAGG+TGG 0.631868 3.4:-95186796 MS.gene031228:CDS
AGTCCCTTCAAATTTAAGCG+AGG 0.644076 3.4:-95186993 MS.gene031228:CDS
GAAGCAGCAATGTTACAAAG+TGG 0.645960 3.4:-95186868 MS.gene031228:CDS
CGAAATCAGCAGAAGAAGCG+GGG 0.650570 3.4:-95186582 MS.gene031228:CDS
GAAGTGATGAGAGGATGTGG+AGG 0.674444 3.4:-95186553 MS.gene031228:CDS
ATCTAGGACAAACTCGATCG+AGG 0.681413 3.4:-95187297 MS.gene031228:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGTTATGAAAAACATTA+TGG + chr3.4:95186512-95186531 None:intergenic 15.0%
!! GAATAAAGATTTCAAGATTT+GGG + chr3.4:95186942-95186961 None:intergenic 20.0%
!! TGAATAAAGATTTCAAGATT+TGG + chr3.4:95186943-95186962 None:intergenic 20.0%
!! TTATGATTAATCATATGCAA+AGG + chr3.4:95187078-95187097 None:intergenic 20.0%
!! TTCAAATTCTCTTCTTTAAA+AGG - chr3.4:95186682-95186701 MS.gene031228:CDS 20.0%
!! TTCAAATTTCGATAAACAAA+CGG + chr3.4:95186731-95186750 None:intergenic 20.0%
! AAAGAAGAGAATTTGAAGAA+AGG + chr3.4:95186679-95186698 None:intergenic 25.0%
! ATTATTACAACACCTTTACT+TGG + chr3.4:95186481-95186500 None:intergenic 25.0%
! CTTAATCTTCAAAACAATCT+AGG - chr3.4:95186595-95186614 MS.gene031228:CDS 25.0%
! TTTGAAGATTAAGAGAGTTT+GGG + chr3.4:95186588-95186607 None:intergenic 25.0%
!! TTTTTGTTCTTTCTTTGTGA+CGG + chr3.4:95186973-95186992 None:intergenic 25.0%
!!! TTTTGAAGATTAAGAGAGTT+TGG + chr3.4:95186589-95186608 None:intergenic 25.0%
CTTGAAATTCCATGAAATTC+AGG + chr3.4:95187213-95187232 None:intergenic 30.0%
TAATCTCTGATGTTGTTACA+AGG - chr3.4:95187103-95187122 MS.gene031228:CDS 30.0%
! ACATGAATTGTATTGACAAG+TGG - chr3.4:95186797-95186816 MS.gene031228:CDS 30.0%
! TCTTTAAAAGGATCCAAACA+AGG - chr3.4:95186694-95186713 MS.gene031228:CDS 30.0%
AGATTCTCTCCTGAATTTCA+TGG - chr3.4:95187201-95187220 MS.gene031228:CDS 35.0%
CTGAGATATCAAAAGTTCCA+AGG - chr3.4:95187298-95187317 MS.gene031228:CDS 35.0%
CTTCTTGATCATGAAGAAAG+TGG - chr3.4:95187018-95187037 MS.gene031228:CDS 35.0%
! TGAAAAGCATGCTTGCATTT+CGG + chr3.4:95186778-95186797 None:intergenic 35.0%
! TTACTTGGAGTAACACCTTT+TGG + chr3.4:95186466-95186485 None:intergenic 35.0%
! TTTGGGTTTAGAAGATGAAC+AGG + chr3.4:95186571-95186590 None:intergenic 35.0%
!! AAAGGTGTTACTCCAAGTAA+AGG - chr3.4:95186466-95186485 MS.gene031228:CDS 35.0%
AGTCCCTTCAAATTTAAGCG+AGG - chr3.4:95186915-95186934 MS.gene031228:CDS 40.0%
ATGTTGTTACAAGGAGTAGG+TGG - chr3.4:95187112-95187131 MS.gene031228:CDS 40.0%
CGATCGAGGTCTATAAATTC+TGG - chr3.4:95186625-95186644 MS.gene031228:CDS 40.0%
CTGATGTTGTTACAAGGAGT+AGG - chr3.4:95187109-95187128 MS.gene031228:CDS 40.0%
GAAGCAGCAATGTTACAAAG+TGG - chr3.4:95187040-95187059 MS.gene031228:CDS 40.0%
GGTCCTCGCTTAAATTTGAA+GGG + chr3.4:95186921-95186940 None:intergenic 40.0%
TCAACTATGTACCTGCAAAG+AGG + chr3.4:95186844-95186863 None:intergenic 40.0%
TGAACTGTTCTCCTTGCAAT+TGG + chr3.4:95187390-95187409 None:intergenic 40.0%
TTGCAAGGAGAACAGTTCAA+TGG - chr3.4:95187391-95187410 MS.gene031228:CDS 40.0%
! TCGTTTACAGCATTGAATCC+TGG - chr3.4:95187258-95187277 MS.gene031228:CDS 40.0%
!! GGTACATTCTAAGCCTTGTT+TGG + chr3.4:95186710-95186729 None:intergenic 40.0%
ACTCTACTTGTCCTCTTTGC+AGG - chr3.4:95186830-95186849 MS.gene031228:CDS 45.0%
ATGAGAGGATGTGGAGGATT+TGG - chr3.4:95187361-95187380 MS.gene031228:CDS 45.0%
ATGGAAGTGATGAGAGGATG+TGG - chr3.4:95187352-95187371 MS.gene031228:CDS 45.0%
CATTGAATCCTGGTGAGAAG+AGG - chr3.4:95187268-95187287 MS.gene031228:CDS 45.0%
GGATTTGGATGCCAATTGCA+AGG - chr3.4:95187376-95187395 MS.gene031228:CDS 45.0%
GGGTCCTCGCTTAAATTTGA+AGG + chr3.4:95186922-95186941 None:intergenic 45.0%
GTCGAAATCAGCAGAAGAAG+CGG - chr3.4:95187324-95187343 MS.gene031228:CDS 45.0%
TCGAAATCAGCAGAAGAAGC+GGG - chr3.4:95187325-95187344 MS.gene031228:CDS 45.0%
! ATCTAGGACAAACTCGATCG+AGG - chr3.4:95186611-95186630 MS.gene031228:CDS 45.0%
!! CTGCTGATTTCGACAAACCT+TGG + chr3.4:95187318-95187337 None:intergenic 45.0%
CGAAATCAGCAGAAGAAGCG+GGG - chr3.4:95187326-95187345 MS.gene031228:CDS 50.0%
GAAGTGATGAGAGGATGTGG+AGG - chr3.4:95187355-95187374 MS.gene031228:CDS 50.0%
GACATACTCCTCTTCTCACC+AGG + chr3.4:95187279-95187298 None:intergenic 50.0%
GGGAGAATGGAAGTGATGAG+AGG - chr3.4:95187346-95187365 MS.gene031228:CDS 50.0%
AGCAGAAGAAGCGGGGAGAA+TGG - chr3.4:95187333-95187352 MS.gene031228:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 95186451 95187479 95186451 ID=MS.gene031228
chr3.4 mRNA 95186451 95187479 95186451 ID=MS.gene031228.t1;Parent=MS.gene031228
chr3.4 exon 95186451 95187479 95186451 ID=MS.gene031228.t1.exon1;Parent=MS.gene031228.t1
chr3.4 CDS 95186451 95187479 95186451 ID=cds.MS.gene031228.t1;Parent=MS.gene031228.t1
Gene Sequence

>MS.gene031228

ATGGCAGAGTTACCAAAAGGTGTTACTCCAAGTAAAGGTGTTGTAATAATAGTACTTTCCATAATGTTTTTCATAACTTTTATCTTACTTGTATATGTCAAATTCTGTCGTGTTACTCCTGTTCATCTTCTAAACCCAAACTCTCTTAATCTTCAAAACAATCTAGGACAAACTCGATCGAGGTCTATAAATTCTGGCGTTGAACAAAAAGTAATTGAAACACTTCCTTTCTTCAAATTCTCTTCTTTAAAAGGATCCAAACAAGGCTTAGAATGTACCGTTTGTTTATCGAAATTTGAAGACGAAGAGATTCTGAGATTGTTGCCGAAATGCAAGCATGCTTTTCACATGAATTGTATTGACAAGTGGCTTGAAAGTCACTCTACTTGTCCTCTTTGCAGGTACATAGTTGAAGAAAGTGACATCAGAAACTTCACTTTTTCATTCAGCTCAAGATTCTTAAGAGTCCCTTCAAATTTAAGCGAGGACCCAAATCTTGAAATCTTTATTCAGAGACAACCGTCACAAAGAAAGAACAAAAAAGAAGAACAAGAACATGAACTTGTTCTTCTTGATCATGAAGAAAGTGGAAGCAGCAATGTTACAAAGTGGAAGCAAGAACAACCTTTGCATATGATTAATCATAAAATATTAATCTCTGATGTTGTTACAAGGAGTAGGTGGAGTGATCTTAATTCTTCAGATTTGTTATCATTGAACAGTGAAATGCTTCATGATATGTCAAGCACGAGATTCTCTCCTGAATTTCATGGAATTTCAAGTGTTCATTCTGATGAAGAAGAAAATTCGTTTACAGCATTGAATCCTGGTGAGAAGAGGAGTATGTCTGAGATATCAAAAGTTCCAAGGTTTGTCGAAATCAGCAGAAGAAGCGGGGAGAATGGAAGTGATGAGAGGATGTGGAGGATTTGGATGCCAATTGCAAGGAGAACAGTTCAATGGTTTGCAAGACAAGAAACAAACTCTGCTCAATTACAACAACATAAACATTTAGTTTCAAACGTTTGA
Protein sequence

>MS.gene031228.t1

MAELPKGVTPSKGVVIIVLSIMFFITFILLVYVKFCRVTPVHLLNPNSLNLQNNLGQTRSRSINSGVEQKVIETLPFFKFSSLKGSKQGLECTVCLSKFEDEEILRLLPKCKHAFHMNCIDKWLESHSTCPLCRYIVEESDIRNFTFSFSSRFLRVPSNLSEDPNLEIFIQRQPSQRKNKKEEQEHELVLLDHEESGSSNVTKWKQEQPLHMINHKILISDVVTRSRWSDLNSSDLLSLNSEMLHDMSSTRFSPEFHGISSVHSDEEENSFTALNPGEKRSMSEISKVPRFVEISRRSGENGSDERMWRIWMPIARRTVQWFARQETNSAQLQQHKHLVSNV