AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031518

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031518.t1 MTR_4g081520 94.631 149 3 1 1 149 1 144 2.70e-99 281
MS.gene031518.t1 MTR_4g081510 69.231 130 33 2 6 135 1 123 1.04e-58 180
MS.gene031518.t1 MTR_7g099940 58.025 81 34 0 57 137 36 116 2.19e-28 102
MS.gene031518.t1 MTR_6g477870 50.000 96 48 0 40 135 79 174 1.84e-26 98.6
MS.gene031518.t1 MTR_7g105260 50.649 77 38 0 58 134 34 110 6.15e-22 85.5
MS.gene031518.t1 MTR_4g109540 41.837 98 52 1 44 136 25 122 1.93e-21 87.4
MS.gene031518.t1 MTR_3g025880 46.512 86 46 0 54 139 42 127 5.49e-21 88.2
MS.gene031518.t1 MTR_1g092860 45.000 80 44 0 58 137 194 273 2.44e-20 84.7
MS.gene031518.t1 MTR_7g013740 44.578 83 46 0 54 136 38 120 2.22e-19 79.3
MS.gene031518.t1 MTR_7g013730 42.353 85 49 0 55 139 39 123 5.15e-19 80.9
MS.gene031518.t1 MTR_4g109540 39.623 106 51 2 44 136 25 130 7.06e-19 80.9
MS.gene031518.t1 MTR_6g477870 46.341 82 44 0 40 121 79 160 2.63e-18 77.0
MS.gene031518.t1 MTR_3g084040 40.594 101 57 2 37 137 19 116 4.80e-18 75.9
MS.gene031518.t1 MTR_3g084040 40.698 86 48 2 37 122 19 101 1.86e-15 68.2
MS.gene031518.t1 MTR_3g029580 39.326 89 50 1 55 143 31 115 2.81e-15 68.2
MS.gene031518.t1 MTR_4g036415 39.796 98 59 0 35 132 430 527 6.38e-15 71.2
MS.gene031518.t1 MTR_4g070080 40.541 74 44 0 51 124 1 74 1.14e-14 66.6
MS.gene031518.t1 MTR_4g070080 40.541 74 44 0 51 124 1 74 2.39e-14 66.6
MS.gene031518.t1 MTR_4g070190 38.356 73 45 0 52 124 4 76 6.85e-14 65.5
MS.gene031518.t1 MTR_2g096560 38.750 80 49 0 56 135 7 86 1.33e-13 62.8
MS.gene031518.t1 MTR_3g462330 40.964 83 49 0 50 132 39 121 8.58e-13 64.7
MS.gene031518.t1 MTR_3g080130 41.463 82 46 2 56 136 36 116 2.03e-12 60.8
MS.gene031518.t1 MTR_4g070140 42.424 66 38 0 59 124 11 76 2.26e-12 62.0
MS.gene031518.t1 MTR_4g063560 28.182 110 71 1 33 134 2 111 4.58e-12 59.3
MS.gene031518.t1 MTR_3g075500 34.951 103 63 1 41 139 187 289 4.87e-12 62.4
MS.gene031518.t1 MTR_7g117495 41.667 60 35 0 56 115 18 77 7.45e-12 61.2
MS.gene031518.t1 MTR_7g117495 41.667 60 35 0 56 115 18 77 8.12e-12 61.2
MS.gene031518.t1 MTR_3g116130 39.080 87 47 2 56 138 29 113 8.25e-12 61.6
MS.gene031518.t1 MTR_3g116130 39.080 87 47 2 56 138 29 113 1.01e-11 61.6
MS.gene031518.t1 MTR_8g024520 43.478 69 35 1 56 120 25 93 1.22e-11 60.8
MS.gene031518.t1 MTR_8g024520 43.478 69 35 1 56 120 25 93 1.24e-11 60.8
MS.gene031518.t1 MTR_8g031560 32.787 122 72 2 22 135 159 278 2.43e-11 60.1
MS.gene031518.t1 MTR_8g070990 38.095 84 48 2 54 134 201 283 2.46e-11 60.8
MS.gene031518.t1 MTR_8g070990 38.095 84 48 2 54 134 201 283 2.61e-11 60.8
MS.gene031518.t1 MTR_8g070990 38.095 84 48 2 54 134 201 283 2.65e-11 60.8
MS.gene031518.t1 MTR_8g070990 38.095 84 48 2 54 134 308 390 2.69e-11 60.8
MS.gene031518.t1 MTR_8g070990 38.095 84 48 2 54 134 308 390 2.74e-11 60.8
MS.gene031518.t1 MTR_8g070990 38.095 84 48 2 54 134 308 390 2.78e-11 60.5
MS.gene031518.t1 MTR_7g094660 34.314 102 66 1 45 146 319 419 3.29e-11 60.5
MS.gene031518.t1 MTR_7g107580 35.417 96 54 1 54 149 346 433 3.64e-11 60.1
MS.gene031518.t1 MTR_7g107580 35.417 96 54 1 54 149 347 434 3.75e-11 60.1
MS.gene031518.t1 MTR_3g029580 42.424 66 38 0 55 120 31 96 4.11e-11 57.4
MS.gene031518.t1 MTR_2g026180 39.744 78 45 1 58 135 6 81 4.99e-11 59.7
MS.gene031518.t1 MTR_4g094235 32.609 92 57 1 42 133 7 93 5.31e-11 59.3
MS.gene031518.t1 MTR_4g094235 32.609 92 57 1 42 133 7 93 5.34e-11 59.3
MS.gene031518.t1 MTR_8g107310 35.294 102 55 1 45 135 189 290 5.71e-11 59.3
MS.gene031518.t1 MTR_1g045510 38.043 92 57 0 44 135 192 283 6.72e-11 58.9
MS.gene031518.t1 MTR_3g014000 35.484 93 49 2 54 139 32 120 7.24e-11 57.0
MS.gene031518.t1 MTR_5g070450 32.609 92 57 1 42 133 7 93 9.48e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031518.t1 AT5G54580 60.135 148 51 2 3 148 9 150 7.84e-53 164
MS.gene031518.t1 AT2G37510 63.014 73 27 0 58 130 36 108 2.64e-27 99.4
MS.gene031518.t1 AT1G73530 50.000 88 44 0 54 141 75 162 1.70e-25 95.9
MS.gene031518.t1 AT2G37510 46.939 98 27 1 58 130 36 133 2.10e-21 85.1
MS.gene031518.t1 AT5G06210 44.706 85 47 0 55 139 33 117 2.29e-19 79.3
MS.gene031518.t1 AT3G20930 40.244 82 49 0 55 136 281 362 3.25e-18 80.1
MS.gene031518.t1 AT5G61030 43.902 82 46 0 54 135 38 119 7.68e-18 78.6
MS.gene031518.t1 AT5G61030 43.902 82 46 0 54 135 38 119 7.68e-18 78.6
MS.gene031518.t1 AT3G46020 36.264 91 58 0 55 145 6 96 1.75e-16 70.5
MS.gene031518.t1 AT1G18630 48.611 72 37 0 56 127 36 107 2.16e-16 71.6
MS.gene031518.t1 AT4G13850 47.561 82 43 0 55 136 34 115 2.31e-16 71.6
MS.gene031518.t1 AT4G13850 44.330 97 51 1 40 136 22 115 2.34e-16 70.9
MS.gene031518.t1 AT3G23830 41.584 101 56 1 37 137 19 116 2.56e-16 71.2
MS.gene031518.t1 AT3G23830 41.584 101 56 1 37 137 19 116 2.56e-16 71.2
MS.gene031518.t1 AT4G13850 47.561 82 43 0 55 136 34 115 2.82e-16 71.6
MS.gene031518.t1 AT4G13850 47.561 82 43 0 55 136 34 115 3.14e-16 71.6
MS.gene031518.t1 AT3G20930 37.931 87 49 1 55 136 281 367 4.79e-16 73.9
MS.gene031518.t1 AT4G39260 39.326 89 54 0 51 139 1 89 1.40e-15 68.2
MS.gene031518.t1 AT2G43370 35.417 96 62 0 44 139 52 147 1.65e-15 70.5
MS.gene031518.t1 AT5G59865 43.750 80 45 0 56 135 26 105 2.24e-15 68.2
MS.gene031518.t1 AT2G43370 35.417 96 62 0 44 139 52 147 1.35e-14 69.7
MS.gene031518.t1 AT1G74230 43.590 78 43 1 55 132 33 109 3.34e-14 68.2
MS.gene031518.t1 AT4G13860 47.143 70 37 0 55 124 2 71 3.81e-14 64.3
MS.gene031518.t1 AT4G39260 41.558 77 45 0 51 127 1 77 7.07e-14 64.7
MS.gene031518.t1 AT5G47320 38.144 97 57 1 40 136 18 111 1.37e-13 65.5
MS.gene031518.t1 AT5G51300 34.234 111 68 2 22 132 451 556 1.43e-13 67.4
MS.gene031518.t1 AT5G51300 34.234 111 68 2 22 132 451 556 1.43e-13 67.4
MS.gene031518.t1 AT5G51300 34.234 111 68 2 22 132 451 556 1.43e-13 67.4
MS.gene031518.t1 AT4G39260 41.558 77 45 0 51 127 1 77 1.72e-13 64.7
MS.gene031518.t1 AT4G24770 36.000 100 64 0 38 137 226 325 3.22e-13 65.9
MS.gene031518.t1 AT4G24770 36.000 100 64 0 38 137 226 325 3.22e-13 65.9
MS.gene031518.t1 AT2G46780 39.535 86 48 1 56 137 22 107 3.73e-13 65.9
MS.gene031518.t1 AT2G46780 39.535 86 48 1 56 137 22 107 3.73e-13 65.9
MS.gene031518.t1 AT3G08000 40.698 86 51 0 57 142 42 127 5.53e-13 62.8
MS.gene031518.t1 AT3G26420 36.170 94 59 1 53 146 4 96 6.38e-13 64.3
MS.gene031518.t1 AT2G27330 29.787 94 64 1 55 146 20 113 1.39e-12 60.8
MS.gene031518.t1 AT1G76460 39.535 86 46 2 56 137 24 107 2.65e-12 63.2
MS.gene031518.t1 AT1G22330 36.471 85 48 2 56 136 17 99 2.75e-12 63.2
MS.gene031518.t1 AT3G53460 39.759 83 50 0 58 140 259 341 4.01e-12 62.8
MS.gene031518.t1 AT3G53460 39.759 83 50 0 58 140 259 341 4.01e-12 62.8
MS.gene031518.t1 AT3G53460 39.759 83 50 0 58 140 259 341 4.01e-12 62.8
MS.gene031518.t1 AT3G53460 39.759 83 50 0 58 140 280 362 4.10e-12 62.8
MS.gene031518.t1 AT1G07350 38.636 88 53 1 44 130 32 119 5.88e-12 59.7
MS.gene031518.t1 AT1G07350 38.636 88 53 1 44 130 32 119 5.88e-12 59.7
MS.gene031518.t1 AT2G37220 41.250 80 47 0 58 137 206 285 6.59e-12 62.0
MS.gene031518.t1 AT1G07350 38.750 80 49 0 53 132 72 151 6.96e-12 62.4
MS.gene031518.t1 AT2G23350 42.466 73 41 1 57 129 135 206 7.77e-12 62.4
MS.gene031518.t1 AT3G11400 33.051 118 63 2 35 136 203 320 8.53e-12 61.6
MS.gene031518.t1 AT1G07350 38.961 77 47 0 54 130 73 149 8.87e-12 59.7
MS.gene031518.t1 AT3G11400 33.051 118 63 2 35 136 176 293 1.02e-11 61.2
MS.gene031518.t1 AT4G34110 29.412 136 82 4 4 128 63 195 1.41e-11 61.6
MS.gene031518.t1 AT4G34110 37.838 74 43 2 56 127 215 287 7.70e-11 59.3
MS.gene031518.t1 AT5G64200 35.065 77 50 0 57 133 17 93 1.46e-11 60.8
MS.gene031518.t1 AT5G64200 35.065 77 50 0 57 133 17 93 1.46e-11 60.8
MS.gene031518.t1 AT1G60000 32.203 118 79 1 19 135 139 256 2.57e-11 60.1
MS.gene031518.t1 AT5G19030 31.579 76 52 0 59 134 80 155 2.78e-11 58.9
MS.gene031518.t1 AT5G47620 36.047 86 52 1 51 133 1 86 3.21e-11 60.5
MS.gene031518.t1 AT5G53720 41.558 77 38 2 51 120 1 77 3.36e-11 57.0
MS.gene031518.t1 AT2G21660 39.394 66 40 0 59 124 11 76 3.92e-11 58.2
MS.gene031518.t1 AT2G21660 39.394 66 40 0 59 124 11 76 5.18e-11 58.2
MS.gene031518.t1 AT4G20030 28.070 114 69 2 32 134 7 118 6.35e-11 57.4
MS.gene031518.t1 AT1G22910 47.273 55 29 0 56 110 13 67 7.68e-11 58.5
MS.gene031518.t1 AT1G22910 47.273 55 29 0 56 110 13 67 7.93e-11 58.5
MS.gene031518.t1 AT4G35785 36.364 88 56 0 50 137 66 153 8.38e-11 58.5
MS.gene031518.t1 AT4G26650 35.870 92 57 1 44 135 3 92 9.10e-11 58.9
MS.gene031518.t1 AT1G07350 37.363 91 56 1 44 133 32 122 9.36e-11 57.8

Find 55 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCATCATCGCCGCCTTC+TGG 0.178462 4.2:+28037568 MS.gene031518:CDS
GAACAACTTCACCGAACTTT+TGG 0.218023 4.2:-28039076 None:intergenic
TCAGGTTACTCTAAGGGATT+TGG 0.331272 4.2:+28039245 MS.gene031518:CDS
TCGAGTCTCAGGTTACTCTA+AGG 0.347313 4.2:+28039238 MS.gene031518:CDS
CGGATGAACATAAATTAATA+AGG 0.358256 4.2:-28040244 None:intergenic
GCTTCTCCGGGTGGTGGTCT+TGG 0.365700 4.2:-28040202 None:intergenic
CGTATTATTTACAGCTTCTC+CGG 0.378906 4.2:-28040215 None:intergenic
GAGAGCTGCAGCTGCGGCGG+CGG 0.386601 4.2:+28037480 MS.gene031518:CDS
AAGTTCGGTGAAGTTGTTCA+TGG 0.397028 4.2:+28039080 MS.gene031518:CDS
AGCACCAACCTCTTCGTCTC+TGG 0.450848 4.2:+28037625 MS.gene031518:CDS
CGGAGAAGCTGTAAATAATA+CGG 0.453875 4.2:+28040216 MS.gene031518:CDS
GATGCTGCTAGGGGCATTGA+AGG 0.455789 4.2:+28039293 MS.gene031518:CDS
AAAGAGTCGGCAGAAACTGC+GGG 0.478130 4.2:-28037506 None:intergenic
TACTTTAGAAGATGCTGCTA+GGG 0.485381 4.2:+28039283 MS.gene031518:CDS
AGGGAAGCATTCCAAAAGTT+CGG 0.494589 4.2:+28039065 MS.gene031518:CDS
ATCTGTCGAGCGGAAGGCGG+CGG 0.498886 4.2:-28037592 None:intergenic
TTTACAGCTTCTCCGGGTGG+TGG 0.499800 4.2:-28040208 None:intergenic
CTACTTTAGAAGATGCTGCT+AGG 0.502306 4.2:+28039282 MS.gene031518:CDS
GTGGTAACAGATCGAGTCTC+AGG 0.506975 4.2:+28039227 MS.gene031518:CDS
GCCGCCTTCCGCTCGACAGA+TGG 0.507477 4.2:+28037594 MS.gene031518:CDS
GATGAGAGCTGCAGCTGCGG+CGG 0.510313 4.2:+28037477 MS.gene031518:CDS
TGGAGAAGAAGCGGTTGGAT+TGG 0.515749 4.2:-28037533 None:intergenic
GATGAGAAAGAAGGGAAAGC+TGG 0.519875 4.2:-28037553 None:intergenic
GGCGGCGATGATGAGAAAGA+AGG 0.531889 4.2:-28037562 None:intergenic
CGAGTCTCAGGTTACTCTAA+GGG 0.537803 4.2:+28039239 MS.gene031518:CDS
GAAGAGGTTGGTGCTAGGCT+CGG 0.548156 4.2:-28037617 None:intergenic
GTATTATTTACAGCTTCTCC+GGG 0.550728 4.2:-28040214 None:intergenic
TCTCATCGTCAAAGCAGCCA+TGG 0.558291 4.2:-28037461 None:intergenic
GCGGAAGGCGGCGGTCCAGA+AGG 0.560349 4.2:-28037583 None:intergenic
GAAGGCGGCGGTCCAGAAGG+CGG 0.560594 4.2:-28037580 None:intergenic
TCGGCCATCTGTCGAGCGGA+AGG 0.561453 4.2:-28037598 None:intergenic
GACGATGAGAGCTGCAGCTG+CGG 0.562915 4.2:+28037474 MS.gene031518:CDS
GCCATCTGTCGAGCGGAAGG+CGG 0.576022 4.2:-28037595 None:intergenic
TGAACATAAATTAATAAGGT+CGG 0.576922 4.2:-28040240 None:intergenic
TGCTAGGGGCATTGAAGGAA+TGG 0.581040 4.2:+28039298 MS.gene031518:CDS
CTCATCGTCAAAGCAGCCAT+GGG 0.581556 4.2:-28037460 None:intergenic
GCGGCGATGATGAGAAAGAA+GGG 0.588249 4.2:-28037561 None:intergenic
GGAGAAGCTGTAAATAATAC+GGG 0.589568 4.2:+28040217 MS.gene031518:CDS
AAAGCTGGAGAAGAAGCGGT+TGG 0.590957 4.2:-28037538 None:intergenic
GAGACGAAGAGGTTGGTGCT+AGG 0.593582 4.2:-28037622 None:intergenic
CGAACCAGAGACGAAGAGGT+TGG 0.593995 4.2:-28037629 None:intergenic
AGGGGCATTGAAGGAATGGA+TGG 0.598515 4.2:+28039302 MS.gene031518:CDS
AGGGAAAGCTGGAGAAGAAG+CGG 0.611069 4.2:-28037542 None:intergenic
TTGGATTGGTGCAAAAGAGT+CGG 0.611688 4.2:-28037519 None:intergenic
AAAAGAGTCGGCAGAAACTG+CGG 0.620147 4.2:-28037507 None:intergenic
TTATTTACAGCTTCTCCGGG+TGG 0.622983 4.2:-28040211 None:intergenic
CATTGAAGGAATGGATGGCA+AGG 0.640049 4.2:+28039307 MS.gene031518:CDS
AAGGCGAACCAGAGACGAAG+AGG 0.640180 4.2:-28037633 None:intergenic
TTTGTCAATCTCAGCAAGGG+TGG 0.647632 4.2:+28039208 MS.gene031518:intron
GTACTACAACAGAGACGCTG+AGG 0.653561 4.2:+28039045 MS.gene031518:CDS
AGGCTCGGCCATCTGTCGAG+CGG 0.658207 4.2:-28037602 None:intergenic
GCAAGACCAAGACCACCACC+CGG 0.665523 4.2:+28040196 MS.gene031518:CDS
TACTACAACAGAGACGCTGA+GGG 0.682055 4.2:+28039046 MS.gene031518:CDS
ACTTTAGAAGATGCTGCTAG+GGG 0.684689 4.2:+28039284 MS.gene031518:CDS
AAGAGTCGGCAGAAACTGCG+GGG 0.717765 4.2:-28037505 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATAATAATAATAATA+AGG - chr4.2:28037675-28037694 None:intergenic 0.0%
!!! AATTTATTTATTTTTATTTA+TGG - chr4.2:28038915-28038934 None:intergenic 0.0%
!! ATTATAATTATTATTGTTGT+TGG + chr4.2:28037685-28037704 MS.gene031518:intron 10.0%
!!! TTTTTTATAATCCTTAAAAT+CGG - chr4.2:28037852-28037871 None:intergenic 10.0%
!!! ATTTATTTTTATTTATGGCA+TGG - chr4.2:28038910-28038929 None:intergenic 15.0%
!!! TTTATTTTTATTTATGGCAT+GGG - chr4.2:28038909-28038928 None:intergenic 15.0%
!! AGTTTAAATGTTTAAAAGGA+TGG - chr4.2:28039114-28039133 None:intergenic 20.0%
!! CAAAAAACATAAAACCATTA+TGG - chr4.2:28039192-28039211 None:intergenic 20.0%
!! GAACAGTTTAAATGTTTAAA+AGG - chr4.2:28039118-28039137 None:intergenic 20.0%
!! TAAATTAAGTTTATCTTAGG+TGG - chr4.2:28039653-28039672 None:intergenic 20.0%
!! TATTATAAAGACATACAATG+AGG - chr4.2:28039611-28039630 None:intergenic 20.0%
!! TGTTTAAGCAATAAGTAAAT+AGG - chr4.2:28038055-28038074 None:intergenic 20.0%
!! TTTATCAATTTCATCATAGA+AGG + chr4.2:28038283-28038302 MS.gene031518:intron 20.0%
!!! ACAAATTGGTTTTGTAAATA+AGG + chr4.2:28038314-28038333 MS.gene031518:intron 20.0%
!!! ACATTGTTATTGTATATGTA+TGG + chr4.2:28040026-28040045 MS.gene031518:intron 20.0%
!!! TTACAAAACCAATTTGTTAA+AGG - chr4.2:28038311-28038330 None:intergenic 20.0%
!!! TTATTTTTATTTATGGCATG+GGG - chr4.2:28038908-28038927 None:intergenic 20.0%
! AAAATTGCAAATGACAATTG+AGG - chr4.2:28038874-28038893 None:intergenic 25.0%
! AAATTAAGAGAATTGAATCC+TGG - chr4.2:28038824-28038843 None:intergenic 25.0%
! AATACATGATTTACAGATTC+GGG - chr4.2:28039909-28039928 None:intergenic 25.0%
! AATTAAGAGAATTGAATCCT+GGG - chr4.2:28038823-28038842 None:intergenic 25.0%
! ACGATATAAGTAGAAATAAG+AGG + chr4.2:28038424-28038443 MS.gene031518:intron 25.0%
! AGATCATTAAATTTGATGTC+AGG - chr4.2:28039852-28039871 None:intergenic 25.0%
! ATAATAAGGAAAAGTTGAGA+AGG - chr4.2:28037661-28037680 None:intergenic 25.0%
! CAATACATGATTTACAGATT+CGG - chr4.2:28039910-28039929 None:intergenic 25.0%
! CAATATTTGAATAGGAAGTT+TGG - chr4.2:28040113-28040132 None:intergenic 25.0%
! CGATATAAGTAGAAATAAGA+GGG + chr4.2:28038425-28038444 MS.gene031518:intron 25.0%
! CGGTAAATTAAGTTTATCTT+AGG - chr4.2:28039656-28039675 None:intergenic 25.0%
! GATATAAGTAGAAATAAGAG+GGG + chr4.2:28038426-28038445 MS.gene031518:intron 25.0%
! GTAAGATTTATTAAACCCTT+TGG + chr4.2:28039329-28039348 MS.gene031518:intron 25.0%
! TAATAAGGAAAAGTTGAGAA+GGG - chr4.2:28037660-28037679 None:intergenic 25.0%
! TACTAATATCAAACCACAAA+GGG - chr4.2:28038139-28038158 None:intergenic 25.0%
! TCAGTAAGCAATATTTGAAT+AGG - chr4.2:28040121-28040140 None:intergenic 25.0%
! TCTTAAAGCACAAATTTGTA+TGG + chr4.2:28039751-28039770 MS.gene031518:intron 25.0%
! TTACTAATATCAAACCACAA+AGG - chr4.2:28038140-28038159 None:intergenic 25.0%
! TTTATGTTCTCATTTGATCT+TGG + chr4.2:28037810-28037829 MS.gene031518:intron 25.0%
!! ACATAAAACCATTATGGTTT+TGG - chr4.2:28039186-28039205 None:intergenic 25.0%
!! ATAAATTTCTTTTCCCTTTG+TGG + chr4.2:28038123-28038142 MS.gene031518:intron 25.0%
!! TCAAAGAAATATGTTTCTGT+TGG - chr4.2:28038634-28038653 None:intergenic 25.0%
AAGAACAGTGAATACATTGT+TGG - chr4.2:28039735-28039754 None:intergenic 30.0%
AAGGATCATTTCATAATCCT+CGG + chr4.2:28038547-28038566 MS.gene031518:intron 30.0%
AATCTACTCCAAAACCATAA+TGG + chr4.2:28039175-28039194 MS.gene031518:intron 30.0%
AGAAAAGCAAAGAACAATCA+TGG - chr4.2:28040088-28040107 None:intergenic 30.0%
ATACATGATTTACAGATTCG+GGG - chr4.2:28039908-28039927 None:intergenic 30.0%
CTTTAATACACTTAATTCCC+AGG + chr4.2:28038803-28038822 MS.gene031518:intron 30.0%
GGGAAAAGAAATTTATACAC+AGG - chr4.2:28038119-28038138 None:intergenic 30.0%
TCATTAAATTTGATGTCAGG+AGG - chr4.2:28039849-28039868 None:intergenic 30.0%
! AGACTTAAGTGTATTTCTGT+CGG + chr4.2:28039374-28039393 MS.gene031518:intron 30.0%
! ATGCAATTTAGAGCAAGTAT+GGG - chr4.2:28038735-28038754 None:intergenic 30.0%
! TTGTTATCTTGGATCTGTTT+TGG + chr4.2:28039401-28039420 MS.gene031518:intron 30.0%
!! TTATGTTTTTGACCATGTTG+AGG + chr4.2:28037775-28037794 MS.gene031518:intron 30.0%
!!! TTTTTTGTCAATCTCAGCAA+GGG + chr4.2:28039205-28039224 MS.gene031518:intron 30.0%
AAATTTGATGTCAGGAGGAA+GGG - chr4.2:28039844-28039863 None:intergenic 35.0%
AGAAGGAGCCTTTAACAAAT+TGG + chr4.2:28038300-28038319 MS.gene031518:intron 35.0%
CAAGTGTCTACTCGAAATAT+GGG + chr4.2:28038571-28038590 MS.gene031518:intron 35.0%
CACTTAAGTCTTCTCGTATA+AGG - chr4.2:28039365-28039384 None:intergenic 35.0%
CATCATATGAAACCTCAACA+TGG - chr4.2:28037790-28037809 None:intergenic 35.0%
CGGAGAAGCTGTAAATAATA+CGG + chr4.2:28040216-28040235 MS.gene031518:CDS 35.0%
CGTATTATTTACAGCTTCTC+CGG - chr4.2:28040218-28040237 None:intergenic 35.0%
CTCATTTGATCTTGGTTGTT+TGG + chr4.2:28037818-28037837 MS.gene031518:intron 35.0%
CTCCAAAAACTGTATTGCAA+GGG - chr4.2:28040152-28040171 None:intergenic 35.0%
GACACAGTCAAGAACTTAAA+AGG - chr4.2:28038459-28038478 None:intergenic 35.0%
GGAGAAGCTGTAAATAATAC+GGG + chr4.2:28040217-28040236 MS.gene031518:CDS 35.0%
GTAAATCTATCTCACTCTCA+CGG - chr4.2:28038227-28038246 None:intergenic 35.0%
GTATTATTTACAGCTTCTCC+GGG - chr4.2:28040217-28040236 None:intergenic 35.0%
TAAATCTATCTCACTCTCAC+GGG - chr4.2:28038226-28038245 None:intergenic 35.0%
TAAATTTGATGTCAGGAGGA+AGG - chr4.2:28039845-28039864 None:intergenic 35.0%
TCAAGTGTCTACTCGAAATA+TGG + chr4.2:28038570-28038589 MS.gene031518:intron 35.0%
TGACGTGTCTTAAACTTTCA+GGG + chr4.2:28039012-28039031 MS.gene031518:intron 35.0%
TGATCTTGGTTGTTTGGTTA+TGG + chr4.2:28037824-28037843 MS.gene031518:intron 35.0%
TTGACGTGTCTTAAACTTTC+AGG + chr4.2:28039011-28039030 MS.gene031518:intron 35.0%
TTTATTTATGGCATGGGGAA+GGG - chr4.2:28038903-28038922 None:intergenic 35.0%
! AATGACAATTGAGGCATCAA+TGG - chr4.2:28038865-28038884 None:intergenic 35.0%
! CATACTTGCTCTAAATTGCA+TGG + chr4.2:28038734-28038753 MS.gene031518:intron 35.0%
! CATGCAATTTAGAGCAAGTA+TGG - chr4.2:28038736-28038755 None:intergenic 35.0%
! TACTTTAGAAGATGCTGCTA+GGG + chr4.2:28039283-28039302 MS.gene031518:CDS 35.0%
! TGGTTATGGATCCGATTTTA+AGG + chr4.2:28037838-28037857 MS.gene031518:intron 35.0%
! TTCCCTTGCAATACAGTTTT+TGG + chr4.2:28040147-28040166 MS.gene031518:intron 35.0%
!! CTAGAGAAAGCATCTAAGAA+AGG + chr4.2:28038528-28038547 MS.gene031518:intron 35.0%
!! CTTGCAATACAGTTTTTGGA+GGG + chr4.2:28040151-28040170 MS.gene031518:intron 35.0%
!! TTTTATTTATGGCATGGGGA+AGG - chr4.2:28038904-28038923 None:intergenic 35.0%
!!! GTTTTTTGTCAATCTCAGCA+AGG + chr4.2:28039204-28039223 MS.gene031518:intron 35.0%
AACACTTGTGTGTCACATTG+TGG + chr4.2:28039536-28039555 MS.gene031518:intron 40.0%
AATTTGATGTCAGGAGGAAG+GGG - chr4.2:28039843-28039862 None:intergenic 40.0%
ACAATGTGACACACGGATTT+CGG + chr4.2:28039486-28039505 MS.gene031518:intron 40.0%
AGGAAAAGTTGAGAAGGGAA+AGG - chr4.2:28037655-28037674 None:intergenic 40.0%
AGTGTGATGTGTATATGCTG+CGG - chr4.2:28039682-28039701 None:intergenic 40.0%
ATTTCACTTGTGTGTCATCG+TGG + chr4.2:28039435-28039454 MS.gene031518:intron 40.0%
CCTCCAAAAACTGTATTGCA+AGG - chr4.2:28040153-28040172 None:intergenic 40.0%
CTCAAAAGAGACCTACAGTA+CGG + chr4.2:28038607-28038626 MS.gene031518:intron 40.0%
CTGTCGGAACATTGTTATCT+TGG + chr4.2:28039390-28039409 MS.gene031518:intron 40.0%
TCTCACGGGTAGTAAAACTA+AGG - chr4.2:28038212-28038231 None:intergenic 40.0%
TTTACTGCAAACTCGAGTAG+AGG - chr4.2:28039778-28039797 None:intergenic 40.0%
! AAGTTCGGTGAAGTTGTTCA+TGG + chr4.2:28039080-28039099 MS.gene031518:CDS 40.0%
! ACTTTAGAAGATGCTGCTAG+GGG + chr4.2:28039284-28039303 MS.gene031518:CDS 40.0%
! ATTTCGGTCTTGTGAAAGAG+AGG + chr4.2:28039502-28039521 MS.gene031518:intron 40.0%
! CAATGGCATGTTGTTTAGAG+AGG - chr4.2:28038848-28038867 None:intergenic 40.0%
! CTACTTTAGAAGATGCTGCT+AGG + chr4.2:28039282-28039301 MS.gene031518:CDS 40.0%
! GAACAACTTCACCGAACTTT+TGG - chr4.2:28039079-28039098 None:intergenic 40.0%
! TCAGGTTACTCTAAGGGATT+TGG + chr4.2:28039245-28039264 MS.gene031518:CDS 40.0%
! TTTCGGTCTTGTGAAAGAGA+GGG + chr4.2:28039503-28039522 MS.gene031518:intron 40.0%
!! AATACAGTTTTTGGAGGGCT+GGG + chr4.2:28040156-28040175 MS.gene031518:intron 40.0%
!! AGGGAAGCATTCCAAAAGTT+CGG + chr4.2:28039065-28039084 MS.gene031518:CDS 40.0%
!! CCTTGCAATACAGTTTTTGG+AGG + chr4.2:28040150-28040169 MS.gene031518:intron 40.0%
!! GTTTTCGCTTTTGAGCCAAA+CGG - chr4.2:28038784-28038803 None:intergenic 40.0%
!! TTGGATTGGTGCAAAAGAGT+CGG - chr4.2:28037522-28037541 None:intergenic 40.0%
AAAAGAGTCGGCAGAAACTG+CGG - chr4.2:28037510-28037529 None:intergenic 45.0%
AGTCTTCTCGTATAAGGTGC+AGG - chr4.2:28039359-28039378 None:intergenic 45.0%
ATCCCACTTGTGTGACATTG+TGG - chr4.2:28039588-28039607 None:intergenic 45.0%
ATGTGTATATGCTGCGGTGT+CGG - chr4.2:28039676-28039695 None:intergenic 45.0%
CACCACAATGTCACACAAGT+GGG + chr4.2:28039583-28039602 MS.gene031518:intron 45.0%
CACTTGTGTGACATTGTGGT+GGG - chr4.2:28039584-28039603 None:intergenic 45.0%
CATTGAAGGAATGGATGGCA+AGG + chr4.2:28039307-28039326 MS.gene031518:CDS 45.0%
CGAGTCTCAGGTTACTCTAA+GGG + chr4.2:28039239-28039258 MS.gene031518:CDS 45.0%
CTTGTGTGTCACATTGTGGT+TGG + chr4.2:28039540-28039559 MS.gene031518:intron 45.0%
GAAATCCGTGTGTCACATTG+TGG - chr4.2:28039487-28039506 None:intergenic 45.0%
GATGAGAAAGAAGGGAAAGC+TGG - chr4.2:28037556-28037575 None:intergenic 45.0%
TACTACAACAGAGACGCTGA+GGG + chr4.2:28039046-28039065 MS.gene031518:CDS 45.0%
TCGAGTCTCAGGTTACTCTA+AGG + chr4.2:28039238-28039257 MS.gene031518:CDS 45.0%
TTATTTACAGCTTCTCCGGG+TGG - chr4.2:28040214-28040233 None:intergenic 45.0%
TTCTCGTATAAGGTGCAGGA+AGG - chr4.2:28039355-28039374 None:intergenic 45.0%
TTTCTGTTGGACCGTACTGT+AGG - chr4.2:28038621-28038640 None:intergenic 45.0%
TTTGTCAATCTCAGCAAGGG+TGG + chr4.2:28039208-28039227 MS.gene031518:intron 45.0%
! ATTGTGGTGGGAATGTCACA+TGG - chr4.2:28039572-28039591 None:intergenic 45.0%
!! CAATACAGTTTTTGGAGGGC+TGG + chr4.2:28040155-28040174 MS.gene031518:intron 45.0%
!! TTCAAAGCACTGTGACCGTT+TGG + chr4.2:28038766-28038785 MS.gene031518:intron 45.0%
AAAGAGTCGGCAGAAACTGC+GGG - chr4.2:28037509-28037528 None:intergenic 50.0%
AAAGCTGGAGAAGAAGCGGT+TGG - chr4.2:28037541-28037560 None:intergenic 50.0%
ACTTGTGTGTCATCGTGGTG+GGG + chr4.2:28039440-28039459 MS.gene031518:intron 50.0%
AGGGAAAGCTGGAGAAGAAG+CGG - chr4.2:28037545-28037564 None:intergenic 50.0%
ATAAGGTGCAGGAAGGCCAA+AGG - chr4.2:28039348-28039367 None:intergenic 50.0%
ATTTATGGCATGGGGAAGGG+AGG - chr4.2:28038900-28038919 None:intergenic 50.0%
CACTTGTGTGTCATCGTGGT+GGG + chr4.2:28039439-28039458 MS.gene031518:intron 50.0%
CCACCACAATGTCACACAAG+TGG + chr4.2:28039582-28039601 MS.gene031518:intron 50.0%
CCACTTGTGTGACATTGTGG+TGG - chr4.2:28039585-28039604 None:intergenic 50.0%
CCCAACCACAATGTGACACA+CGG + chr4.2:28039479-28039498 MS.gene031518:intron 50.0%
CCGTGTGTCACATTGTGGTT+GGG - chr4.2:28039482-28039501 None:intergenic 50.0%
GCGGCGATGATGAGAAAGAA+GGG - chr4.2:28037564-28037583 None:intergenic 50.0%
GTACTACAACAGAGACGCTG+AGG + chr4.2:28039045-28039064 MS.gene031518:CDS 50.0%
GTGGTAACAGATCGAGTCTC+AGG + chr4.2:28039227-28039246 MS.gene031518:CDS 50.0%
TAAGGTGCAGGAAGGCCAAA+GGG - chr4.2:28039347-28039366 None:intergenic 50.0%
TCACTTGTGTGTCATCGTGG+TGG + chr4.2:28039438-28039457 MS.gene031518:intron 50.0%
TCCGTGTGTCACATTGTGGT+TGG - chr4.2:28039483-28039502 None:intergenic 50.0%
TGGAGAAGAAGCGGTTGGAT+TGG - chr4.2:28037536-28037555 None:intergenic 50.0%
TTCGAGTAGACACTTGACCG+AGG - chr4.2:28038567-28038586 None:intergenic 50.0%
!! AGGGGCATTGAAGGAATGGA+TGG + chr4.2:28039302-28039321 MS.gene031518:CDS 50.0%
!! TGCTAGGGGCATTGAAGGAA+TGG + chr4.2:28039298-28039317 MS.gene031518:CDS 50.0%
AAGAGTCGGCAGAAACTGCG+GGG - chr4.2:28037508-28037527 None:intergenic 55.0%
AAGGCGAACCAGAGACGAAG+AGG - chr4.2:28037636-28037655 None:intergenic 55.0%
AGCACCAACCTCTTCGTCTC+TGG + chr4.2:28037625-28037644 MS.gene031518:CDS 55.0%
CGAACCAGAGACGAAGAGGT+TGG - chr4.2:28037632-28037651 None:intergenic 55.0%
GGCGGCGATGATGAGAAAGA+AGG - chr4.2:28037565-28037584 None:intergenic 55.0%
TTCTCATCATCGCCGCCTTC+TGG + chr4.2:28037568-28037587 MS.gene031518:CDS 55.0%
TTTACAGCTTCTCCGGGTGG+TGG - chr4.2:28040211-28040230 None:intergenic 55.0%
! CATCGTGGTGGGGATTTCAC+AGG + chr4.2:28039450-28039469 MS.gene031518:intron 55.0%
!! GAAGAGGTTGGTGCTAGGCT+CGG - chr4.2:28037620-28037639 None:intergenic 55.0%
!! GAGACGAAGAGGTTGGTGCT+AGG - chr4.2:28037625-28037644 None:intergenic 55.0%
!! GATGCTGCTAGGGGCATTGA+AGG + chr4.2:28039293-28039312 MS.gene031518:CDS 55.0%
GACGATGAGAGCTGCAGCTG+CGG + chr4.2:28037474-28037493 MS.gene031518:CDS 60.0%
GCAAGACCAAGACCACCACC+CGG + chr4.2:28040196-28040215 MS.gene031518:CDS 60.0%
AGGCTCGGCCATCTGTCGAG+CGG - chr4.2:28037605-28037624 None:intergenic 65.0%
ATCTGTCGAGCGGAAGGCGG+CGG - chr4.2:28037595-28037614 None:intergenic 65.0%
GATGAGAGCTGCAGCTGCGG+CGG + chr4.2:28037477-28037496 MS.gene031518:CDS 65.0%
GCCATCTGTCGAGCGGAAGG+CGG - chr4.2:28037598-28037617 None:intergenic 65.0%
TCGGCCATCTGTCGAGCGGA+AGG - chr4.2:28037601-28037620 None:intergenic 65.0%
! GCTTCTCCGGGTGGTGGTCT+TGG - chr4.2:28040205-28040224 None:intergenic 65.0%
GAAGGCGGCGGTCCAGAAGG+CGG - chr4.2:28037583-28037602 None:intergenic 70.0%
GCCGCCTTCCGCTCGACAGA+TGG + chr4.2:28037594-28037613 MS.gene031518:CDS 70.0%
GAGAGCTGCAGCTGCGGCGG+CGG + chr4.2:28037480-28037499 MS.gene031518:CDS 75.0%
GCGGAAGGCGGCGGTCCAGA+AGG - chr4.2:28037586-28037605 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 28037463 28040252 28037463 ID=MS.gene031518
chr4.2 mRNA 28037463 28040252 28037463 ID=MS.gene031518.t1;Parent=MS.gene031518
chr4.2 exon 28037463 28037646 28037463 ID=MS.gene031518.t1.exon1;Parent=MS.gene031518.t1
chr4.2 CDS 28037463 28037646 28037463 ID=cds.MS.gene031518.t1;Parent=MS.gene031518.t1
chr4.2 exon 28039033 28039101 28039033 ID=MS.gene031518.t1.exon2;Parent=MS.gene031518.t1
chr4.2 CDS 28039033 28039101 28039033 ID=cds.MS.gene031518.t1;Parent=MS.gene031518.t1
chr4.2 exon 28039222 28039328 28039222 ID=MS.gene031518.t1.exon3;Parent=MS.gene031518.t1
chr4.2 CDS 28039222 28039328 28039222 ID=cds.MS.gene031518.t1;Parent=MS.gene031518.t1
chr4.2 exon 28040163 28040252 28040163 ID=MS.gene031518.t1.exon4;Parent=MS.gene031518.t1
chr4.2 CDS 28040163 28040252 28040163 ID=cds.MS.gene031518.t1;Parent=MS.gene031518.t1
Gene Sequence

>MS.gene031518

ATGGCTGCTTTGACGATGAGAGCTGCAGCTGCGGCGGCGGTGCCCCGCAGTTTCTGCCGACTCTTTTGCACCAATCCAACCGCTTCTTCTCCAGCTTTCCCTTCTTTCTCATCATCGCCGCCTTCTGGACCGCCGCCTTCCGCTCGACAGATGGCCGAGCCTAGCACCAACCTCTTCGTCTCTGGTTCGCCTTTCCCTTCTCAACTTTTCCTTATTATTATTATTATAATTATTATTGTTGTTGGTTGAATGCTAGATAGTTATTGTGATTTATGAATTGAAAGTGTATGTTTTGATATTATTGTTTGTGTTTTATGTTTTTGACCATGTTGAGGTTTCATATGATGTTTATGTTCTCATTTGATCTTGGTTGTTTGGTTATGGATCCGATTTTAAGGATTATAAAAAAGTGATTTTGATACTCAATTATTTAGATATTATTTTTTAGAGATTTTCAATAAACGCATAATCTGTTTTGTGTTTGTTTTACAAAGTTTAAATGAAAATTTATAGAATTGATTTTTTTTTTTTTAATGAAAAATCAGAATTTTCAATCAAATACACAGATGCTATTAAAGCTGTTGAATTTCCTATTTACTTATTGCTTAAACAACTTATCTTTGTTTAAAATAACAGCTTATGCTTATGCATTTCCTGTGTATAAATTTCTTTTCCCTTTGTGGTTTGATATTAGTAATTCTTTGAATAAGTGATTTGTTTTCATGTATTCAATTCATAAGTAGACTCCTTAGTTTTACTACCCGTGAGAGTGAGATAGATTTACATATATGACATCTGTACACTCATGCAAAAAGTAATTTTTATCAATTTCATCATAGAAGGAGCCTTTAACAAATTGGTTTTGTAAATAAGGAAGCTTTTGAAATGATTAATTAACTAGAAAATATACTGTATGAATTTTAACTCGCGACTCTTTTATCTGTATTGCTAGATAAATCAAACGATATAAGTAGAAATAAGAGGGGATTTTATCCTTTTAAGTTCTTGACTGTGTCTATAGAAATGTTTAGTTGCGTAAAGTAAAAATTAGATAGATAATATAATCTAGAGAAAGCATCTAAGAAAGGATCATTTCATAATCCTCGGTCAAGTGTCTACTCGAAATATGGGTATTGTCATCAAACTCAAAAGAGACCTACAGTACGGTCCAACAGAAACATATTTCTTTGAAGCAATCTCTGAAAATACGAGTGCTTTTCACATTTTTTGATTGCAGTGTTGAATCACTACTAGCTATTTTGATTTTAACCCATACTTGCTCTAAATTGCATGGTTTCGTGACTTCAAAGCACTGTGACCGTTTGGCTCAAAAGCGAAAACTTTAATACACTTAATTCCCAGGATTCAATTCTCTTAATTTTCCTCTCTAAACAACATGCCATTGATGCCTCAATTGTCATTTGCAATTTTTATCCTCCCTTCCCCATGCCATAAATAAAAATAAATAAATTTGTATATGTCATTGCTAATGTTTAGCTTTATTTCAATGCAATTTGTCACTTGAAATATTCAGTTGACTGTTGATTTTTGACGTGTCTTAAACTTTCAGGGCTTAGTAAACGTACTACAACAGAGACGCTGAGGGAAGCATTCCAAAAGTTCGGTGAAGTTGTTCATGGTTTGTTCTCCATCCTTTTAAACATTTAAACTGTTCTGCTATTTATGCTTTTGCTATGCAATGATGACTTGAAAATCTACTCCAAAACCATAATGGTTTTATGTTTTTTGTCAATCTCAGCAAGGGTGGTAACAGATCGAGTCTCAGGTTACTCTAAGGGATTTGGTTTCGTGAAATATGCTACTTTAGAAGATGCTGCTAGGGGCATTGAAGGAATGGATGGCAAGGTAAGATTTATTAAACCCTTTGGCCTTCCTGCACCTTATACGAGAAGACTTAAGTGTATTTCTGTCGGAACATTGTTATCTTGGATCTGTTTTGGATTTTTCACTGATTTCACTTGTGTGTCATCGTGGTGGGGATTTCACAGGACATTGCCCAACCACAATGTGACACACGGATTTCGGTCTTGTGAAAGAGAGGGAAGAAAACAAAACACTTGTGTGTCACATTGTGGTTGGACAATGCCATGTGACATTCCCACCACAATGTCACACAAGTGGGATCCTCATTGTATGTCTTTATAATAAAAAGTTAGTTCAGTATGACCACCTAAGATAAACTTAATTTACCGACACCGCAGCATATACACATCACACTAGAAGATTGAACTCTAATTGCTTGACATTGCCAACAATGTATTCACTGTTCTTAAAGCACAAATTTGTATGGACCTCTACTCGAGTTTGCAGTAAATCGCATTTACTGTTATTTGAACTCAAGTTTTCTCAATTTTCTCCCCTTCCTCCTGACATCAAATTTAATGATCTAACTCTTCAATATTTAGAGTATTTGCTTAATCACCCCGAATCTGTAAATCATGTATTGATTGCTGAGCTCAAATAAATATGCATTGTTTGACTTAACAACTGCTATAACTGAGTTTTGTTGTCAGTTGCATGTTGTTTTAATGAATTTTTCTTAACATTGTTATTGTATATGTATGGAAAACTTTCTTATGTAGTTCATCTACACAATTGATGCCATGATTGTTCTTTGCTTTTCTTGCCAAACTTCCTATTCAAATATTGCTTACTGATATGTTTTCCCTTGCAATACAGTTTTTGGAGGGCTGGGTTATATTTGCAGAGTATGCAAGACCAAGACCACCACCCGGAGAAGCTGTAAATAATACGGGCCGACCTTATTAA
Protein sequence

>MS.gene031518.t1

MAALTMRAAAAAAVPRSFCRLFCTNPTASSPAFPSFSSSPPSGPPPSARQMAEPSTNLFVSGLSKRTTTETLREAFQKFGEVVHARVVTDRVSGYSKGFGFVKYATLEDAARGIEGMDGKFLEGWVIFAEYARPRPPPGEAVNNTGRPY