AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031519

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031519.t1 MTR_4g081510 87.027 185 20 1 1 185 1 181 1.83e-112 318
MS.gene031519.t1 MTR_4g081520 72.269 119 30 1 9 127 15 130 6.01e-52 163
MS.gene031519.t1 MTR_6g477870 48.101 79 41 0 50 128 97 175 5.43e-24 93.6
MS.gene031519.t1 MTR_7g099940 51.282 78 38 0 50 127 37 114 6.66e-24 92.0
MS.gene031519.t1 MTR_7g105260 46.835 79 42 0 50 128 34 112 1.50e-19 80.9
MS.gene031519.t1 MTR_1g092860 39.362 94 50 2 50 140 194 283 4.32e-18 80.1
MS.gene031519.t1 MTR_7g013740 46.914 81 41 1 50 128 42 122 7.69e-18 76.6
MS.gene031519.t1 MTR_3g025880 45.977 87 45 1 43 127 39 125 8.72e-17 77.4
MS.gene031519.t1 MTR_7g013730 43.590 78 44 0 50 127 42 119 1.44e-16 75.5
MS.gene031519.t1 MTR_4g109540 39.000 100 57 2 33 128 25 124 1.62e-16 75.5
MS.gene031519.t1 MTR_6g477870 46.875 64 34 0 50 113 97 160 4.17e-16 72.4
MS.gene031519.t1 MTR_4g109540 43.210 81 44 1 50 128 52 132 3.34e-15 72.0
MS.gene031519.t1 MTR_3g084040 42.308 78 44 1 50 127 38 114 1.15e-14 68.2
MS.gene031519.t1 MTR_3g029580 41.558 77 45 0 50 126 34 110 1.18e-13 65.1
MS.gene031519.t1 MTR_3g084040 38.750 80 47 2 33 112 22 99 7.03e-13 62.4
MS.gene031519.t1 MTR_8g031560 33.058 121 73 2 16 128 159 279 1.92e-12 64.3
MS.gene031519.t1 MTR_4g070190 35.526 76 49 0 41 116 1 76 1.06e-11 60.5
MS.gene031519.t1 MTR_3g080130 33.913 115 72 3 30 141 18 131 1.07e-11 60.1
MS.gene031519.t1 MTR_2g096560 32.099 81 55 0 50 130 9 89 1.25e-11 58.5
MS.gene031519.t1 MTR_8g107310 37.662 77 48 0 52 128 215 291 3.20e-11 60.8
MS.gene031519.t1 MTR_4g070080 37.879 66 41 0 51 116 9 74 5.19e-11 57.8
MS.gene031519.t1 MTR_4g070140 40.909 66 39 0 51 116 11 76 8.90e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031519.t1 AT5G54580 56.618 136 55 1 6 141 18 149 2.15e-46 150
MS.gene031519.t1 AT2G37510 47.706 109 55 1 50 158 36 142 2.66e-23 90.5
MS.gene031519.t1 AT1G73530 45.238 84 46 0 46 129 75 158 2.62e-21 86.3
MS.gene031519.t1 AT2G37510 38.806 134 55 2 50 158 36 167 1.61e-17 76.3
MS.gene031519.t1 AT5G61030 45.570 79 43 0 50 128 42 120 7.90e-17 77.0
MS.gene031519.t1 AT5G61030 45.570 79 43 0 50 128 42 120 7.90e-17 77.0
MS.gene031519.t1 AT5G06210 43.590 78 44 0 50 127 36 113 1.04e-16 73.6
MS.gene031519.t1 AT3G46020 38.158 76 47 0 50 125 9 84 2.76e-16 71.2
MS.gene031519.t1 AT3G20930 37.179 78 49 0 50 127 284 361 1.39e-15 73.9
MS.gene031519.t1 AT3G23830 43.210 81 46 0 50 130 37 117 3.77e-15 69.3
MS.gene031519.t1 AT3G23830 43.210 81 46 0 50 130 37 117 3.77e-15 69.3
MS.gene031519.t1 AT1G18630 47.143 70 37 0 50 119 38 107 1.25e-13 65.5
MS.gene031519.t1 AT3G20930 34.940 83 49 1 50 127 284 366 1.81e-13 67.8
MS.gene031519.t1 AT4G13850 36.111 108 55 2 16 116 3 103 6.08e-13 63.2
MS.gene031519.t1 AT4G13850 36.111 108 55 2 16 116 3 103 9.82e-13 63.2
MS.gene031519.t1 AT4G13850 36.111 108 55 2 16 116 3 103 1.18e-12 63.2
MS.gene031519.t1 AT4G13850 36.111 108 55 2 16 116 3 103 1.30e-12 63.2
MS.gene031519.t1 AT5G59865 41.026 78 46 0 50 127 28 105 1.86e-12 61.6
MS.gene031519.t1 AT4G13860 43.284 67 38 0 50 116 5 71 4.94e-12 59.7
MS.gene031519.t1 AT1G74230 40.000 75 44 1 50 124 36 109 5.05e-12 63.2
MS.gene031519.t1 AT3G08000 43.284 67 38 0 50 116 43 109 5.72e-12 60.8
MS.gene031519.t1 AT2G27330 32.877 73 49 0 50 122 23 95 2.39e-11 58.5
MS.gene031519.t1 AT2G43370 32.530 83 56 0 43 125 59 141 2.42e-11 61.6
MS.gene031519.t1 AT5G19030 30.263 76 53 0 51 126 80 155 3.08e-11 59.7
MS.gene031519.t1 AT2G43370 32.530 83 56 0 43 125 59 141 5.36e-11 59.3

Find 52 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGTTTGGTGAAGTTAAAC+AGG 0.263827 4.2:+28043697 MS.gene031519:CDS
GACCGGTGACGAAGAGAATT+TGG 0.272558 4.2:-28042914 None:intergenic
GAGGCTATAGCATTGGTTCT+TGG 0.297566 4.2:-28044634 None:intergenic
TCAGGTTACTCTAAGGGATT+TGG 0.331272 4.2:+28043876 MS.gene031519:CDS
TCGTGTCTCAGGTTACTCTA+AGG 0.355649 4.2:+28043869 MS.gene031519:CDS
TTCATTTCACCGTTCTTCTC+TGG 0.361360 4.2:-28044709 None:intergenic
GGGATGGTTCAGTATGTGTC+TGG 0.374916 4.2:-28044657 None:intergenic
CGGTGCCCCGCGGTTTCTGC+CGG 0.374929 4.2:+28042793 MS.gene031519:CDS
TTATCCTAATCTTTGTCCAT+TGG 0.396575 4.2:-28044799 None:intergenic
GAAAAGCCGGCAGAAACCGC+GGG 0.416020 4.2:-28042799 None:intergenic
CGAACCAATGGACAAAGATT+AGG 0.427675 4.2:+28044795 MS.gene031519:CDS
GATGCAGCTAAGGGCATTCA+AGG 0.474539 4.2:+28043924 MS.gene031519:CDS
AGAGCAGAAGTAGCTCTAGA+AGG 0.482168 4.2:-28042873 None:intergenic
AAGGCGGCGTTGAAGCGAAC+GGG 0.486768 4.2:-28042854 None:intergenic
TACTTCAGAAGATGCAGCTA+AGG 0.490832 4.2:+28043914 MS.gene031519:CDS
GTGGTGGCAGATCGTGTCTC+AGG 0.492116 4.2:+28043858 MS.gene031519:CDS
GATGAGAGCTACAGCGGCAG+CGG 0.497516 4.2:+28042773 None:intergenic
TTGGCTTCGTGCAGAAAAGC+CGG 0.502812 4.2:-28042812 None:intergenic
GTGACGAAGAGAATTTGGTT+GGG 0.517626 4.2:-28042909 None:intergenic
AACCAAATTCTCTTCGTCAC+CGG 0.521059 4.2:+28042912 MS.gene031519:CDS
TCTTCAGGTTCGGAGTGGGA+TGG 0.524520 4.2:-28044673 None:intergenic
GCCGATCTCAGCAAGGGTGG+TGG 0.532889 4.2:+28043842 MS.gene031519:intron
ACAGCGGCAGCGGTGCCCCG+CGG 0.533814 4.2:+28042783 MS.gene031519:CDS
GGAAACTGCTGGCAAGAAGA+AGG 0.538907 4.2:+28044735 MS.gene031519:CDS
GGTGACGAAGAGAATTTGGT+TGG 0.541116 4.2:-28042910 None:intergenic
CGTGTCTCAGGTTACTCTAA+GGG 0.541129 4.2:+28043870 MS.gene031519:CDS
GAAGAACGGTGAAATGAAGA+AGG 0.549581 4.2:+28044714 MS.gene031519:CDS
GGATGGTTCAGTATGTGTCT+GGG 0.554245 4.2:-28044656 None:intergenic
AAAACGACGCCAGAGAAGAA+CGG 0.554308 4.2:+28044700 MS.gene031519:CDS
GAAGGCGGCGTTGAAGCGAA+CGG 0.562134 4.2:-28042855 None:intergenic
CATTCAAGGCATGGATGGCA+AGG 0.576598 4.2:+28043938 MS.gene031519:CDS
TGTCTGGGAGGCTATAGCAT+TGG 0.576971 4.2:-28044641 None:intergenic
AAGGGCATTCAAGGCATGGA+TGG 0.580521 4.2:+28043933 MS.gene031519:CDS
AGAAAAGCCGGCAGAAACCG+CGG 0.588918 4.2:-28042800 None:intergenic
AGCTAAGGGCATTCAAGGCA+TGG 0.591683 4.2:+28043929 MS.gene031519:CDS
ACTTCAGAAGATGCAGCTAA+GGG 0.597665 4.2:+28043915 MS.gene031519:CDS
AGCTACTTCTGCTCTAAAGA+CGG 0.598572 4.2:+28042881 MS.gene031519:CDS
GGTAGTTGCAGACGAACCAA+TGG 0.606038 4.2:+28044783 MS.gene031519:CDS
GTTGAAGCGAACGGGACAGT+GGG 0.608011 4.2:-28042846 None:intergenic
AAGTTTGGTGAAGTTAAACA+GGG 0.611008 4.2:+28043698 MS.gene031519:CDS
TTTGCCGATCTCAGCAAGGG+TGG 0.614124 4.2:+28043839 MS.gene031519:intron
CGTTGAAGCGAACGGGACAG+TGG 0.621721 4.2:-28042847 None:intergenic
CGGGACAGTGGGAGAAGACG+CGG 0.622686 4.2:-28042835 None:intergenic
GAAATGAAGAAGGAAACTGC+TGG 0.640066 4.2:+28044724 MS.gene031519:CDS
TGGTTCAGTATGTGTCTGGG+AGG 0.643151 4.2:-28044653 None:intergenic
AAAAGCCGGCAGAAACCGCG+GGG 0.647049 4.2:-28042798 None:intergenic
ACAGTGGGAGAAGACGCGGT+TGG 0.653547 4.2:-28042831 None:intergenic
GTACTACATCAGAGACGCTG+AGG 0.664662 4.2:+28043663 MS.gene031519:CDS
TTTGATGATGAGAGCTACAG+CGG 0.670929 4.2:+28042767 None:intergenic
TACTACATCAGAGACGCTGA+GGG 0.671573 4.2:+28043664 MS.gene031519:CDS
TACTTCTGCTCTAAAGACGG+CGG 0.680130 4.2:+28042884 MS.gene031519:CDS
GCAGAAGTAGCTCTAGAAGG+CGG 0.685533 4.2:-28042870 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAGGAGTAAATATTAAT+CGG + chr4.2:28043214-28043233 MS.gene031519:intron 20.0%
!! CAAATGTTAAAATTTACCAA+GGG - chr4.2:28043736-28043755 None:intergenic 20.0%
!! CATAATATTCATTTGATTGT+GGG + chr4.2:28043604-28043623 MS.gene031519:intron 20.0%
!! TCAAATGTTAAAATTTACCA+AGG - chr4.2:28043737-28043756 None:intergenic 20.0%
!! TCATAATATTCATTTGATTG+TGG + chr4.2:28043603-28043622 MS.gene031519:intron 20.0%
!! TGTTAATGTAAACTGTAAAT+TGG + chr4.2:28042964-28042983 MS.gene031519:intron 20.0%
!! TTATGAAAGACAAATTGAAT+TGG - chr4.2:28043589-28043608 None:intergenic 20.0%
!! TTTATGCAATTTGTAACAAA+CGG - chr4.2:28043101-28043120 None:intergenic 20.0%
!!! TGTTTTTTTATGTTCAACAA+AGG + chr4.2:28043128-28043147 MS.gene031519:intron 20.0%
! AATCATAGCTGAAACTATAA+TGG + chr4.2:28043809-28043828 MS.gene031519:intron 25.0%
! TAAACAAAGTTTATCTTAGG+TGG - chr4.2:28044094-28044113 None:intergenic 25.0%
! TAAATGTGTCATAACTTTCA+GGG + chr4.2:28043630-28043649 MS.gene031519:intron 25.0%
! TGTTTAAAGAGGGAAATTAA+GGG - chr4.2:28043465-28043484 None:intergenic 25.0%
! TTAAATGTGTCATAACTTTC+AGG + chr4.2:28043629-28043648 MS.gene031519:intron 25.0%
!! CTCTTTTCTTTTTCTTATCA+TGG - chr4.2:28043261-28043280 None:intergenic 25.0%
!!! CTTCATTTTTATTTATGGCA+GGG - chr4.2:28043534-28043553 None:intergenic 25.0%
!!! GACAGCTTCATTTTTATTTA+TGG - chr4.2:28043539-28043558 None:intergenic 25.0%
!!! GTTCTCATTTGATTTGATTT+AGG + chr4.2:28043059-28043078 MS.gene031519:intron 25.0%
!!! TTCATTTTTATTTATGGCAG+GGG - chr4.2:28043533-28043552 None:intergenic 25.0%
AAAGGATCATTTCTTCTTAG+AGG + chr4.2:28043146-28043165 MS.gene031519:intron 30.0%
AAGAATAGTGAATGCATTGT+TGG - chr4.2:28044176-28044195 None:intergenic 30.0%
AATGACAGTTGTAACATGAA+TGG - chr4.2:28043490-28043509 None:intergenic 30.0%
AATGCATGATTTACAGATTC+AGG - chr4.2:28044350-28044369 None:intergenic 30.0%
AGATCATTGAATTTCATGTC+AGG - chr4.2:28044293-28044312 None:intergenic 30.0%
AGTAATAAGCAATTACTGAC+CGG - chr4.2:28042934-28042953 None:intergenic 30.0%
AGTAATCTCTCAAACGATTT+GGG + chr4.2:28043286-28043305 MS.gene031519:intron 30.0%
ATGAATGGTGTGTTTAAAGA+GGG - chr4.2:28043475-28043494 None:intergenic 30.0%
ATGCATGATTTACAGATTCA+GGG - chr4.2:28044349-28044368 None:intergenic 30.0%
CAGCAATAATTGAATCGAAT+CGG - chr4.2:28044548-28044567 None:intergenic 30.0%
CGGTAAACAAAGTTTATCTT+AGG - chr4.2:28044097-28044116 None:intergenic 30.0%
GAAAAAGAAGATAACTGCAA+AGG + chr4.2:28044762-28044781 MS.gene031519:CDS 30.0%
GTGTTTAAAGAGGGAAATTA+AGG - chr4.2:28043466-28043485 None:intergenic 30.0%
GTTTAAAGAGGGAAATTAAG+GGG - chr4.2:28043464-28043483 None:intergenic 30.0%
TAGTAATCTCTCAAACGATT+TGG + chr4.2:28043285-28043304 MS.gene031519:intron 30.0%
TCTTAAAGCACGAATTTGTA+TGG + chr4.2:28044192-28044211 MS.gene031519:intron 30.0%
TTTCTTATCATGGCAAATGT+TGG - chr4.2:28043251-28043270 None:intergenic 30.0%
! AAAGTTTGGTGAAGTTAAAC+AGG + chr4.2:28043697-28043716 MS.gene031519:CDS 30.0%
! AAGTTTGGTGAAGTTAAACA+GGG + chr4.2:28043698-28043717 MS.gene031519:CDS 30.0%
! TTCTAAAAACTGCATTGCAA+AGG - chr4.2:28044584-28044603 None:intergenic 30.0%
!! ACATTGTTGTTGTTTATGTC+TGG + chr4.2:28044453-28044472 MS.gene031519:intron 30.0%
!! ACTGATTTGAGTTTATGACT+TGG + chr4.2:28043181-28043200 MS.gene031519:intron 30.0%
!! AGGGAAGCATTTAAAAAGTT+TGG + chr4.2:28043683-28043702 MS.gene031519:CDS 30.0%
!! GCTTCATTTTTATTTATGGC+AGG - chr4.2:28043535-28043554 None:intergenic 30.0%
!! TGATTTAGGTTGTCTTGTTA+CGG + chr4.2:28043073-28043092 MS.gene031519:intron 30.0%
!! TTTGCAATGCAGTTTTTAGA+AGG + chr4.2:28044583-28044602 MS.gene031519:intron 30.0%
!!! TCGTATTTCATGTTTTGATC+AGG + chr4.2:28043010-28043029 MS.gene031519:intron 30.0%
AGAAAAGCAAAGAACAACCA+TGG - chr4.2:28044515-28044534 None:intergenic 35.0%
AGTGTGATGTGTATATGTTG+CGG - chr4.2:28044123-28044142 None:intergenic 35.0%
CATGAATGGTGTGTTTAAAG+AGG - chr4.2:28043476-28043495 None:intergenic 35.0%
CTGTCAGAATATTGTTGTCT+TGG + chr4.2:28044020-28044039 MS.gene031519:intron 35.0%
GGTTGAGATCAAAATAGCTA+GGG - chr4.2:28043405-28043424 None:intergenic 35.0%
GTAATCTCTCAAACGATTTG+GGG + chr4.2:28043287-28043306 MS.gene031519:intron 35.0%
TAAATAGCTGAACGAGTGAA+TGG - chr4.2:28043761-28043780 None:intergenic 35.0%
TCATTGAATTTCATGTCAGG+AGG - chr4.2:28044290-28044309 None:intergenic 35.0%
TTTACTGCAAACTCGAATAG+AGG - chr4.2:28044219-28044238 None:intergenic 35.0%
! AGCTGTCTATTGCATTGATA+AGG + chr4.2:28043552-28043571 MS.gene031519:intron 35.0%
! TAATCTCTCAAACGATTTGG+GGG + chr4.2:28043288-28043307 MS.gene031519:intron 35.0%
!! ACAACTCACTCTAGTCTTTT+GGG - chr4.2:28043357-28043376 None:intergenic 35.0%
!! CGTTTTCTTTTCTTCAGGTT+CGG - chr4.2:28044686-28044705 None:intergenic 35.0%
!! GATTTAGGTTGTCTTGTTAC+GGG + chr4.2:28043074-28043093 MS.gene031519:intron 35.0%
!!! ATGTTTTGATCAGGTTGTTG+AGG + chr4.2:28043019-28043038 MS.gene031519:intron 35.0%
AAATTAAGGGGAACGCTGTT+TGG - chr4.2:28043452-28043471 None:intergenic 40.0%
AACCAAATTCTCTTCGTCAC+CGG + chr4.2:28042912-28042931 MS.gene031519:CDS 40.0%
AATTTCATGTCAGGAGGAAG+GGG - chr4.2:28044284-28044303 None:intergenic 40.0%
ACTTCAGAAGATGCAGCTAA+GGG + chr4.2:28043915-28043934 MS.gene031519:CDS 40.0%
ACTTGGTTCAATCTCGACAA+AGG + chr4.2:28043198-28043217 MS.gene031519:intron 40.0%
ATCTCAACCCACAATTTGCA+TGG + chr4.2:28043416-28043435 MS.gene031519:intron 40.0%
ATGTGTATATGTTGCGGTGT+CGG - chr4.2:28044117-28044136 None:intergenic 40.0%
CAGTATTGTGTTACATGTGC+AGG - chr4.2:28043990-28044009 None:intergenic 40.0%
GAAATGAAGAAGGAAACTGC+TGG + chr4.2:28044724-28044743 MS.gene031519:CDS 40.0%
GAAGAACGGTGAAATGAAGA+AGG + chr4.2:28044714-28044733 MS.gene031519:CDS 40.0%
GAATTTCATGTCAGGAGGAA+GGG - chr4.2:28044285-28044304 None:intergenic 40.0%
GGGTTGAGATCAAAATAGCT+AGG - chr4.2:28043406-28043425 None:intergenic 40.0%
GTGACGAAGAGAATTTGGTT+GGG - chr4.2:28042912-28042931 None:intergenic 40.0%
TACTTCAGAAGATGCAGCTA+AGG + chr4.2:28043914-28043933 MS.gene031519:CDS 40.0%
TGAATTTCATGTCAGGAGGA+AGG - chr4.2:28044286-28044305 None:intergenic 40.0%
TTCATTTCACCGTTCTTCTC+TGG - chr4.2:28044712-28044731 None:intergenic 40.0%
TTTATTTATGGCAGGGGAAG+AGG - chr4.2:28043527-28043546 None:intergenic 40.0%
! TCAGGTTACTCTAAGGGATT+TGG + chr4.2:28043876-28043895 MS.gene031519:CDS 40.0%
!! AGCTACTTCTGCTCTAAAGA+CGG + chr4.2:28042881-28042900 MS.gene031519:CDS 40.0%
!! AGCTCAACTGGTTTGAGATA+AGG + chr4.2:28043317-28043336 MS.gene031519:intron 40.0%
!! CAATGCAGTTTTTAGAAGGC+TGG + chr4.2:28044587-28044606 MS.gene031519:intron 40.0%
!! CACAACTCACTCTAGTCTTT+TGG - chr4.2:28043358-28043377 None:intergenic 40.0%
!! CATCTACAAAGTTGATGCCA+TGG + chr4.2:28044495-28044514 MS.gene031519:intron 40.0%
!! GCTCAACTGGTTTGAGATAA+GGG + chr4.2:28043318-28043337 MS.gene031519:intron 40.0%
AAAACGACGCCAGAGAAGAA+CGG + chr4.2:28044700-28044719 MS.gene031519:CDS 45.0%
AACCCACAATTTGCATGGTG+TGG + chr4.2:28043421-28043440 MS.gene031519:intron 45.0%
AGGGTATGTTCTTCATCCCT+TGG + chr4.2:28043717-28043736 MS.gene031519:intron 45.0%
ATTGTGTTACATGTGCAGGC+AGG - chr4.2:28043986-28044005 None:intergenic 45.0%
ATTTCATGTCAGGAGGAAGG+GGG - chr4.2:28044283-28044302 None:intergenic 45.0%
CACCACACCATGCAAATTGT+GGG - chr4.2:28043426-28043445 None:intergenic 45.0%
CGTGTCTCAGGTTACTCTAA+GGG + chr4.2:28043870-28043889 MS.gene031519:CDS 45.0%
GGATGGTTCAGTATGTGTCT+GGG - chr4.2:28044659-28044678 None:intergenic 45.0%
GGTGACGAAGAGAATTTGGT+TGG - chr4.2:28042913-28042932 None:intergenic 45.0%
TACTACATCAGAGACGCTGA+GGG + chr4.2:28043664-28043683 MS.gene031519:CDS 45.0%
TCACCACACCATGCAAATTG+TGG - chr4.2:28043427-28043446 None:intergenic 45.0%
TCGTGTCTCAGGTTACTCTA+AGG + chr4.2:28043869-28043888 MS.gene031519:CDS 45.0%
! TCTCAAACGATTTGGGGGTT+TGG + chr4.2:28043293-28043312 MS.gene031519:intron 45.0%
!! AGAGCAGAAGTAGCTCTAGA+AGG - chr4.2:28042876-28042895 None:intergenic 45.0%
!! CTTTTCTTCAGGTTCGGAGT+GGG - chr4.2:28044680-28044699 None:intergenic 45.0%
!! GAGGCTATAGCATTGGTTCT+TGG - chr4.2:28044637-28044656 None:intergenic 45.0%
!! GGCGTCGTTTTCTTTTCTTC+AGG - chr4.2:28044691-28044710 None:intergenic 45.0%
!! TACTTCTGCTCTAAAGACGG+CGG + chr4.2:28042884-28042903 MS.gene031519:CDS 45.0%
!! TCTTTTCTTCAGGTTCGGAG+TGG - chr4.2:28044681-28044700 None:intergenic 45.0%
ACATGTGCAGGCAGGCTAAA+GGG - chr4.2:28043978-28043997 None:intergenic 50.0%
CATTCAAGGCATGGATGGCA+AGG + chr4.2:28043938-28043957 MS.gene031519:CDS 50.0%
GACCGGTGACGAAGAGAATT+TGG - chr4.2:28042917-28042936 None:intergenic 50.0%
GGAAACTGCTGGCAAGAAGA+AGG + chr4.2:28044735-28044754 MS.gene031519:CDS 50.0%
GGGATGGTTCAGTATGTGTC+TGG - chr4.2:28044660-28044679 None:intergenic 50.0%
GGTAGTTGCAGACGAACCAA+TGG + chr4.2:28044783-28044802 MS.gene031519:CDS 50.0%
GTACTACATCAGAGACGCTG+AGG + chr4.2:28043663-28043682 MS.gene031519:CDS 50.0%
TACATGTGCAGGCAGGCTAA+AGG - chr4.2:28043979-28043998 None:intergenic 50.0%
TGGTTCAGTATGTGTCTGGG+AGG - chr4.2:28044656-28044675 None:intergenic 50.0%
TGTCTGGGAGGCTATAGCAT+TGG - chr4.2:28044644-28044663 None:intergenic 50.0%
TTGGCTTCGTGCAGAAAAGC+CGG - chr4.2:28042815-28042834 None:intergenic 50.0%
TTTCATGTCAGGAGGAAGGG+GGG - chr4.2:28044282-28044301 None:intergenic 50.0%
!! AAGGGCATTCAAGGCATGGA+TGG + chr4.2:28043933-28043952 MS.gene031519:CDS 50.0%
!! AGCTAAGGGCATTCAAGGCA+TGG + chr4.2:28043929-28043948 MS.gene031519:CDS 50.0%
!! GATGCAGCTAAGGGCATTCA+AGG + chr4.2:28043924-28043943 MS.gene031519:CDS 50.0%
!! GCAGAAGTAGCTCTAGAAGG+CGG - chr4.2:28042873-28042892 None:intergenic 50.0%
AGAAAAGCCGGCAGAAACCG+CGG - chr4.2:28042803-28042822 None:intergenic 55.0%
CCCTTGCTGAGATCGGCAAA+CGG - chr4.2:28043839-28043858 None:intergenic 55.0%
CCGTTTGCCGATCTCAGCAA+GGG + chr4.2:28043836-28043855 MS.gene031519:intron 55.0%
GTTGAAGCGAACGGGACAGT+GGG - chr4.2:28042849-28042868 None:intergenic 55.0%
TCCGTTTGCCGATCTCAGCA+AGG + chr4.2:28043835-28043854 MS.gene031519:intron 55.0%
TTCATGTCAGGAGGAAGGGG+GGG - chr4.2:28044281-28044300 None:intergenic 55.0%
TTTGCCGATCTCAGCAAGGG+TGG + chr4.2:28043839-28043858 MS.gene031519:intron 55.0%
! TCTTCAGGTTCGGAGTGGGA+TGG - chr4.2:28044676-28044695 None:intergenic 55.0%
! TGGGGGTTTGGTAGCTCAAC+TGG + chr4.2:28043305-28043324 MS.gene031519:intron 55.0%
AAAAGCCGGCAGAAACCGCG+GGG - chr4.2:28042801-28042820 None:intergenic 60.0%
ACAGTGGGAGAAGACGCGGT+TGG - chr4.2:28042834-28042853 None:intergenic 60.0%
CGTTGAAGCGAACGGGACAG+TGG - chr4.2:28042850-28042869 None:intergenic 60.0%
GAAAAGCCGGCAGAAACCGC+GGG - chr4.2:28042802-28042821 None:intergenic 60.0%
GCCACCACCCTTGCTGAGAT+CGG - chr4.2:28043846-28043865 None:intergenic 60.0%
GTGGTGGCAGATCGTGTCTC+AGG + chr4.2:28043858-28043877 MS.gene031519:CDS 60.0%
! AAGGCGGCGTTGAAGCGAAC+GGG - chr4.2:28042857-28042876 None:intergenic 60.0%
! GAAGGCGGCGTTGAAGCGAA+CGG - chr4.2:28042858-28042877 None:intergenic 60.0%
CGGGACAGTGGGAGAAGACG+CGG - chr4.2:28042838-28042857 None:intergenic 65.0%
GCCGATCTCAGCAAGGGTGG+TGG + chr4.2:28043842-28043861 MS.gene031519:intron 65.0%
CGGTGCCCCGCGGTTTCTGC+CGG + chr4.2:28042793-28042812 MS.gene031519:CDS 75.0%
! ACAGCGGCAGCGGTGCCCCG+CGG + chr4.2:28042783-28042802 MS.gene031519:CDS 80.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 28042774 28044816 28042774 ID=MS.gene031519
chr4.2 mRNA 28042774 28044816 28042774 ID=MS.gene031519.t1;Parent=MS.gene031519
chr4.2 exon 28042774 28042933 28042774 ID=MS.gene031519.t1.exon1;Parent=MS.gene031519.t1
chr4.2 CDS 28042774 28042933 28042774 ID=cds.MS.gene031519.t1;Parent=MS.gene031519.t1
chr4.2 exon 28043651 28043719 28043651 ID=MS.gene031519.t1.exon2;Parent=MS.gene031519.t1
chr4.2 CDS 28043651 28043719 28043651 ID=cds.MS.gene031519.t1;Parent=MS.gene031519.t1
chr4.2 exon 28043853 28043959 28043853 ID=MS.gene031519.t1.exon3;Parent=MS.gene031519.t1
chr4.2 CDS 28043853 28043959 28043853 ID=cds.MS.gene031519.t1;Parent=MS.gene031519.t1
chr4.2 exon 28044595 28044816 28044595 ID=MS.gene031519.t1.exon4;Parent=MS.gene031519.t1
chr4.2 CDS 28044595 28044816 28044595 ID=cds.MS.gene031519.t1;Parent=MS.gene031519.t1
Gene Sequence

>MS.gene031519

ATGAGAGCTACAGCGGCAGCGGTGCCCCGCGGTTTCTGCCGGCTTTTCTGCACGAAGCCAACCGCGTCTTCTCCCACTGTCCCGTTCGCTTCAACGCCGCCTTCTAGAGCTACTTCTGCTCTAAAGACGGCGGAGCCCAACCAAATTCTCTTCGTCACCGGTCAGTAATTGCTTATTACTATCTATCTATTGTTAATGTAAACTGTAAATTGGAGTGTATGTTTTGATATTGTTGTTCGTATTTCATGTTTTGATCAGGTTGTTGAGGTTTTATTTCATGTATATGTTCTCATTTGATTTGATTTAGGTTGTCTTGTTACGGGTCCGTTTGTTACAAATTGCATAAAAAAAAAATGTTTTTTTATGTTCAACAAAGGATCATTTCTTCTTAGAGGCTGAGTAGTTCAACTGATTTGAGTTTATGACTTGGTTCAATCTCGACAAAGGAGTAAATATTAATCGGATAACTAATTACCAACATTTGCCATGATAAGAAAAAGAAAAGAGTAAATAGTAATCTCTCAAACGATTTGGGGGTTTGGTAGCTCAACTGGTTTGAGATAAGGGATTAAAGAAGTCTCCCAAAAGACTAGAGTGAGTTGTGCATTGTTTGATTGCAGTGTTAACTCCCTAGCTATTTTGATCTCAACCCACAATTTGCATGGTGTGGTGACGCCAAACAGCGTTCCCCTTAATTTCCCTCTTTAAACACACCATTCATGTTACAACTGTCATTTTCAATTTTTATCTCCTCTTCCCCTGCCATAAATAAAAATGAAGCTGTCTATTGCATTGATAAGGTCATTCTTTGTCCAATTCAATTTGTCTTTCATAATATTCATTTGATTGTGGGTTTTAAATGTGTCATAACTTTCAGGGCTTAACAAACGTACTACATCAGAGACGCTGAGGGAAGCATTTAAAAAGTTTGGTGAAGTTAAACAGGGTATGTTCTTCATCCCTTGGTAAATTTTAACATTTGATCCATTCACTCGTTCAGCTATTTATGCTTTTACTATGCGATGATGTCTTAAAAATCATAGCTGAAACTATAATGGTTTTCCGTTTGCCGATCTCAGCAAGGGTGGTGGCAGATCGTGTCTCAGGTTACTCTAAGGGATTTGGTTACGTGAAATATGCTACTTCAGAAGATGCAGCTAAGGGCATTCAAGGCATGGATGGCAAGGTAAAATTTATTAAACCCTTTAGCCTGCCTGCACATGTAACACAATACTGAAATGTATTTCTGTCAGAATATTGTTGTCTTGGATTTGTCTCTGCATTTGTTTATTTATGCAAAATAAAAATTTAATATGACCACCTAAGATAAACTTTGTTTACCGACACCGCAACATATACACATCACACTAGAAGACTGAACTCTAATTGCTTGACATTGCCAACAATGCATTCACTATTCTTAAAGCACGAATTTGTATGGACCTCTATTCGAGTTTGCAGTAAATCTCATTTACTATTATTTGAACTCAAGTTTTCTCAATTTCCCCCCCTTCCTCCTGACATGAAATTCAATGATCTAACTCTTCAATATTTAGAGTATTTATTTAATCACCCTGAATCTGTAAATCATGCATTGATCAGTTAGCTCAAATTAATATGCATCTTTTTACATGACAATCAGAATGTAATTATGTGTTTTGTTTTCATGAATTTTCGTGACATTGTTGTTGTTTATGTCTGGAAAACTTTTTTGTGTAGTTCATCTACAAAGTTGATGCCATGGTTGTTCTTTGCTTTTCTTACAAAATTTCCGATTCGATTCAATTATTGCTGACTGATATGTTTTCCTTTGCAATGCAGTTTTTAGAAGGCTGGATTGTATTTGCAGAGTATGCAAGACCAAGAACCAATGCTATAGCCTCCCAGACACATACTGAACCATCCCACTCCGAACCTGAAGAAAAGAAAACGACGCCAGAGAAGAACGGTGAAATGAAGAAGGAAACTGCTGGCAAGAAGAAGGACAAGAAAAAGAAGATAACTGCAAAGGTAGTTGCAGACGAACCAATGGACAAAGATTAG
Protein sequence

>MS.gene031519.t1

MRATAAAVPRGFCRLFCTKPTASSPTVPFASTPPSRATSALKTAEPNQILFVTGLNKRTTSETLREAFKKFGEVKQARVVADRVSGYSKGFGYVKYATSEDAAKGIQGMDGKFLEGWIVFAEYARPRTNAIASQTHTEPSHSEPEEKKTTPEKNGEMKKETAGKKKDKKKKITAKVVADEPMDKD