AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032574

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032574.t1 MTR_6g005760 95.294 595 28 0 28 622 10 604 0.0 1141
MS.gene032574.t1 MTR_6g005760 96.008 476 19 0 147 622 1 476 0.0 942
MS.gene032574.t1 MTR_4g077880 73.628 565 148 1 58 621 46 610 0.0 837
MS.gene032574.t1 MTR_6g005760 94.393 428 24 0 28 455 10 437 0.0 813
MS.gene032574.t1 MTR_5g020530 60.862 580 211 5 50 618 50 624 0.0 678
MS.gene032574.t1 MTR_4g077880 68.593 398 125 0 58 455 46 443 0.0 548
MS.gene032574.t1 MTR_8g099065 28.502 207 118 5 380 581 570 751 9.90e-16 81.6
MS.gene032574.t1 MTR_3g112260 33.553 152 93 5 380 524 172 322 2.42e-15 79.0
MS.gene032574.t1 MTR_3g089100 30.622 209 111 6 380 581 968 1149 2.61e-15 80.5
MS.gene032574.t1 MTR_3g089100 30.622 209 111 6 380 581 939 1120 2.82e-15 80.1
MS.gene032574.t1 MTR_3g089100 30.622 209 111 6 380 581 934 1115 2.89e-15 80.1
MS.gene032574.t1 MTR_3g092080 34.868 152 92 5 379 525 120 269 3.14e-15 78.2
MS.gene032574.t1 MTR_3g092080 34.868 152 92 5 379 525 120 269 3.61e-15 78.2
MS.gene032574.t1 MTR_3g117230 33.553 152 93 5 380 524 175 325 4.60e-15 78.2
MS.gene032574.t1 MTR_7g101870 29.577 213 116 8 375 579 510 696 5.97e-15 79.0
MS.gene032574.t1 MTR_7g101870 30.872 149 98 4 380 524 242 389 6.84e-14 75.5
MS.gene032574.t1 MTR_1g088730 29.665 209 113 6 380 581 969 1150 6.23e-15 79.3
MS.gene032574.t1 MTR_1g101870 35.714 154 91 4 380 527 594 745 7.56e-15 78.6
MS.gene032574.t1 MTR_1g088750 29.665 209 113 6 380 581 926 1107 8.04e-15 79.0
MS.gene032574.t1 MTR_1g088750 29.665 209 113 6 380 581 975 1156 8.51e-15 78.6
MS.gene032574.t1 MTR_1g101870 35.714 154 91 4 380 527 542 693 8.75e-15 78.6
MS.gene032574.t1 MTR_1g063380 29.245 212 118 8 375 579 509 695 1.00e-14 78.2
MS.gene032574.t1 MTR_1g063380 30.872 149 98 4 380 524 241 388 1.95e-13 73.9
MS.gene032574.t1 MTR_5g009840 28.502 207 118 5 380 581 552 733 1.43e-14 77.8
MS.gene032574.t1 MTR_4g053350 28.019 207 121 5 380 582 162 344 1.47e-14 76.6
MS.gene032574.t1 MTR_4g053350 28.019 207 121 5 380 582 162 344 1.65e-14 76.6
MS.gene032574.t1 MTR_4g094270 31.683 202 105 8 382 575 194 370 1.69e-14 76.3
MS.gene032574.t1 MTR_2g095210 31.875 160 101 5 375 527 437 595 1.82e-14 77.4
MS.gene032574.t1 MTR_2g024440 33.766 154 97 4 382 532 260 411 1.90e-14 76.6
MS.gene032574.t1 MTR_2g024440 33.766 154 97 4 382 532 343 494 1.97e-14 77.0
MS.gene032574.t1 MTR_2g024440 33.766 154 97 4 382 532 342 493 2.00e-14 77.0
MS.gene032574.t1 MTR_3g092070 32.911 158 87 4 379 525 117 266 2.00e-14 75.9
MS.gene032574.t1 MTR_6g033740 32.468 154 96 4 380 527 536 687 2.87e-14 76.6
MS.gene032574.t1 MTR_2g086160 27.363 201 118 5 380 576 167 343 3.32e-14 74.7
MS.gene032574.t1 MTR_1g083330 35.032 157 94 5 375 524 198 353 3.46e-14 75.5
MS.gene032574.t1 MTR_4g033235 35.032 157 94 5 375 524 199 354 3.84e-14 75.5
MS.gene032574.t1 MTR_2g086160 27.053 207 123 5 380 582 167 349 4.99e-14 75.1
MS.gene032574.t1 MTR_3g092070 33.766 154 91 4 379 525 117 266 6.84e-14 73.6
MS.gene032574.t1 MTR_4g011810 27.488 211 117 6 380 579 277 462 7.97e-14 74.7
MS.gene032574.t1 MTR_1g099480 33.333 153 96 4 380 527 505 656 3.29e-13 73.2
MS.gene032574.t1 MTR_2g095320 30.201 149 99 4 380 524 185 332 4.10e-13 72.8
MS.gene032574.t1 MTR_8g056860 31.847 157 89 4 382 524 507 659 2.84e-12 70.5
MS.gene032574.t1 MTR_6g023875 28.436 211 114 8 378 579 415 597 3.05e-12 70.1
MS.gene032574.t1 MTR_8g099065 32.308 130 82 3 380 504 570 698 4.68e-12 69.7
MS.gene032574.t1 MTR_7g089040 27.830 212 122 7 374 579 715 901 7.02e-12 69.3
MS.gene032574.t1 MTR_7g075520 26.872 227 147 6 380 589 247 471 2.14e-11 67.0
MS.gene032574.t1 MTR_8g099605 31.515 165 97 5 375 524 434 597 3.42e-11 67.0
MS.gene032574.t1 MTR_2g007010 33.537 164 97 7 380 534 273 433 3.68e-11 66.6
MS.gene032574.t1 MTR_8g099605 31.515 165 97 5 375 524 193 356 4.18e-11 66.6
MS.gene032574.t1 MTR_7g088830 27.652 264 149 12 366 618 684 916 5.84e-11 66.2
MS.gene032574.t1 MTR_7g010800 28.641 206 113 7 382 579 243 422 6.03e-11 65.9
MS.gene032574.t1 MTR_3g023890 27.315 216 138 7 385 596 426 626 7.14e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032574.t1 AT3G03060 74.826 576 139 3 48 620 54 626 0.0 892
MS.gene032574.t1 AT5G16930 71.026 604 163 3 28 620 36 638 0.0 879
MS.gene032574.t1 AT2G18330 63.558 579 202 5 46 617 52 628 0.0 699
MS.gene032574.t1 AT4G36580 61.257 573 215 4 50 618 54 623 0.0 682
MS.gene032574.t1 AT3G19740 31.481 216 109 9 380 587 738 922 9.52e-19 91.3
MS.gene032574.t1 AT1G50140 30.841 214 113 7 380 587 726 910 2.19e-18 90.1
MS.gene032574.t1 AT1G50140 30.841 214 113 7 380 587 726 910 2.19e-18 90.1
MS.gene032574.t1 AT1G50140 30.841 214 113 7 380 587 748 932 2.26e-18 90.1
MS.gene032574.t1 AT1G50140 30.841 214 113 7 380 587 748 932 2.26e-18 90.1
MS.gene032574.t1 AT1G50140 30.841 214 113 7 380 587 748 932 2.26e-18 90.1
MS.gene032574.t1 AT5G19990 32.673 202 103 8 382 575 196 372 5.59e-16 80.9
MS.gene032574.t1 AT5G19990 32.673 202 103 8 382 575 196 372 5.59e-16 80.9
MS.gene032574.t1 AT5G19990 32.673 202 103 8 382 575 138 314 7.19e-16 80.1
MS.gene032574.t1 AT5G20000 32.673 202 103 8 382 575 196 372 8.44e-16 80.5
MS.gene032574.t1 AT3G53230 30.047 213 115 9 375 579 509 695 1.13e-15 81.3
MS.gene032574.t1 AT3G53230 30.872 149 98 4 380 524 241 388 1.10e-13 75.1
MS.gene032574.t1 AT1G45000 33.553 152 93 5 380 524 172 322 1.25e-15 79.7
MS.gene032574.t1 AT4G04180 36.601 153 79 5 382 524 362 506 1.38e-15 80.5
MS.gene032574.t1 AT4G04180 36.601 153 79 5 382 524 357 501 1.46e-15 80.5
MS.gene032574.t1 AT5G03340 30.516 213 114 9 375 579 508 694 4.35e-15 79.3
MS.gene032574.t1 AT5G03340 30.872 149 98 4 380 524 240 387 2.30e-13 73.9
MS.gene032574.t1 AT5G53540 35.065 154 89 4 379 525 120 269 5.65e-15 77.8
MS.gene032574.t1 AT1G02890 29.665 209 113 6 380 581 978 1159 5.90e-15 79.3
MS.gene032574.t1 AT1G53750 27.799 259 151 10 290 524 105 351 6.88e-15 77.8
MS.gene032574.t1 AT5G43010 32.895 152 94 5 380 524 172 322 7.34e-15 77.4
MS.gene032574.t1 AT4G28000 32.692 156 93 4 380 527 553 704 7.57e-15 78.6
MS.gene032574.t1 AT4G28000 32.692 156 93 4 380 527 553 704 8.13e-15 78.6
MS.gene032574.t1 AT4G28000 32.692 156 93 4 380 527 551 702 9.22e-15 78.6
MS.gene032574.t1 AT1G53780 34.810 158 95 5 374 524 388 544 1.41e-14 77.4
MS.gene032574.t1 AT1G53780 34.810 158 95 5 374 524 367 523 1.47e-14 77.4
MS.gene032574.t1 AT1G53780 34.810 158 95 5 374 524 367 523 1.47e-14 77.4
MS.gene032574.t1 AT3G09840 29.577 213 117 9 375 579 508 695 1.49e-14 77.8
MS.gene032574.t1 AT3G09840 30.872 149 98 4 380 524 240 387 2.40e-13 73.9
MS.gene032574.t1 AT1G53780 34.810 158 95 5 374 524 366 522 1.51e-14 77.4
MS.gene032574.t1 AT1G64110 32.051 156 94 4 380 527 554 705 1.52e-14 77.8
MS.gene032574.t1 AT1G64110 32.051 156 94 4 380 527 554 705 1.52e-14 77.8
MS.gene032574.t1 AT1G64110 32.051 156 94 4 380 527 552 703 1.60e-14 77.8
MS.gene032574.t1 AT1G64110 32.051 156 94 4 380 527 552 703 1.60e-14 77.8
MS.gene032574.t1 AT1G64110 32.051 156 94 4 380 527 549 700 1.71e-14 77.4
MS.gene032574.t1 AT4G02480 29.665 209 113 6 380 581 997 1178 2.01e-14 77.4
MS.gene032574.t1 AT5G52882 33.117 154 95 4 380 527 550 701 2.15e-14 77.4
MS.gene032574.t1 AT4G27680 36.129 155 86 6 379 525 117 266 2.47e-14 75.9
MS.gene032574.t1 AT1G09100 35.976 164 83 6 375 524 198 353 9.83e-14 73.9
MS.gene032574.t1 AT3G05530 34.395 157 95 5 375 524 199 354 2.77e-13 72.8
MS.gene032574.t1 AT4G24860 28.708 209 115 6 380 581 852 1033 2.90e-13 73.9
MS.gene032574.t1 AT4G24860 28.708 209 115 6 380 581 854 1035 3.00e-13 73.6
MS.gene032574.t1 AT2G27600 26.570 207 124 5 380 582 164 346 3.26e-13 72.4
MS.gene032574.t1 AT4G24860 28.708 209 115 6 380 581 827 1008 3.60e-13 73.6
MS.gene032574.t1 AT1G80350 27.488 211 117 6 380 579 271 456 3.94e-13 72.8
MS.gene032574.t1 AT4G24860 28.708 209 115 6 380 581 850 1031 4.02e-13 73.2
MS.gene032574.t1 AT1G79560 26.073 303 169 12 344 619 485 759 6.30e-13 72.8
MS.gene032574.t1 AT1G79560 26.073 303 169 12 344 619 485 759 6.88e-13 72.4
MS.gene032574.t1 AT1G79560 26.073 303 169 12 344 619 485 759 6.95e-13 72.4
MS.gene032574.t1 AT3G02450 34.228 149 91 5 382 525 368 514 1.31e-12 71.2
MS.gene032574.t1 AT1G62130 29.064 203 110 6 380 581 787 956 2.23e-12 70.9
MS.gene032574.t1 AT1G62130 29.064 203 110 6 380 581 780 949 2.26e-12 70.9
MS.gene032574.t1 AT3G27120 28.436 211 114 7 378 579 433 615 2.91e-12 70.1
MS.gene032574.t1 AT3G56690 28.155 206 118 7 380 580 417 597 3.48e-11 67.0

Find 148 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGGAATTACATCGATTT+TGG 0.148803 6.3:+1783328 MS.gene032574:CDS
GCCATTGTCTTCCCTGTTCC+TGG 0.205135 6.3:-1787809 None:intergenic
TCAAATAATGGAAAGGGTTT+TGG 0.219162 6.3:+1787674 MS.gene032574:CDS
GGGAAGGTGCACGGGTTATT+TGG 0.224243 6.3:+1787522 MS.gene032574:intron
GGGATATGTTGATAGAATAT+TGG 0.232274 6.3:+1787544 MS.gene032574:CDS
CCAGAATCTGTTTCAAATAA+TGG 0.246142 6.3:+1787662 MS.gene032574:CDS
GGAAGGTGCACGGGTTATTT+GGG 0.252159 6.3:+1787523 MS.gene032574:CDS
CCATTATTTGAAACAGATTC+TGG 0.258534 6.3:-1787662 None:intergenic
GCCATTCCTTCTTCATCTTC+AGG 0.266934 6.3:+1784129 MS.gene032574:CDS
AATACTACCTTTGACCATAT+TGG 0.291759 6.3:+1786824 MS.gene032574:CDS
CCACAAAAGATAGAGATTAA+AGG 0.293367 6.3:+1790342 MS.gene032574:CDS
TGCTTCGTCATATGCTAAAA+AGG 0.298502 6.3:+1784257 MS.gene032574:CDS
TAAACCCCTATTGGACTTCT+TGG 0.299729 6.3:-1789801 None:intergenic
GTCCTAGAATTTCCCTTGCC+TGG 0.304734 6.3:+1790219 MS.gene032574:CDS
CCGTGAAGTCGTAGATTATA+AGG 0.320677 6.3:+1790500 MS.gene032574:CDS
AATCCAAGGAGTGCTTCTTC+TGG 0.327589 6.3:+1784183 MS.gene032574:CDS
GCTCTTGGTTCCTTACTCTA+TGG 0.329815 6.3:-1786553 None:intergenic
GCATCAACAAAGAATAAAAT+TGG 0.330618 6.3:+1790530 MS.gene032574:CDS
GGCGGTGGTGGCGGCGGTTA+AGG 0.332054 6.3:-1784085 None:intergenic
CCCCAGGCAAGGGAAATTCT+AGG 0.335721 6.3:-1790221 None:intergenic
CAGTTTCGCTATTTCCCTTC+CGG 0.338361 6.3:-1790415 None:intergenic
GATCCTGAACCTCTTGAAAA+AGG 0.346940 6.3:+1784210 MS.gene032574:CDS
GGATATGTTGATAGAATATT+GGG 0.347459 6.3:+1787545 MS.gene032574:CDS
GGAGTGACTGCTCTGGCAGC+CGG 0.356065 6.3:+1787260 MS.gene032574:CDS
GGAGACAAGGCAAGCTGAAT+TGG 0.367167 6.3:+1784493 MS.gene032574:CDS
TGATTTCATGGAGGTGAGTT+GGG 0.372960 6.3:+1783311 None:intergenic
AGAGACGGTGGCGGTGGTGG+CGG 0.374239 6.3:-1784094 None:intergenic
CAAGCTGGATCAAGAAAATC+TGG 0.381037 6.3:+1790294 MS.gene032574:CDS
AAGTCGAGAAAGGGTGCTTT+TGG 0.394444 6.3:-1787633 None:intergenic
TAATCAAATGAAAGCACAAT+TGG 0.397234 6.3:+1784535 MS.gene032574:CDS
TTGGGTTGAAATCAGGTTAA+GGG 0.399695 6.3:+1783347 MS.gene032574:CDS
GAGTGACTGCTCTGGCAGCC+GGG 0.400139 6.3:+1787261 MS.gene032574:CDS
TCCTAGAATTTCCCTTGCCT+GGG 0.409654 6.3:+1790220 MS.gene032574:CDS
ATTGGGCCAAGAAGTCCAAT+AGG 0.417609 6.3:+1789795 MS.gene032574:CDS
CTTATTACGATCTGTTAGAA+TGG 0.422073 6.3:-1787219 None:intergenic
GATACACGAGTTGTTTGATT+GGG 0.423129 6.3:+1789778 MS.gene032574:CDS
GAAAGAGACGGTGGCGGTGG+TGG 0.423161 6.3:-1784097 None:intergenic
TTTGGGTTGAAATCAGGTTA+AGG 0.423578 6.3:+1783346 MS.gene032574:CDS
GACGGTGGCGGTGGTGGCGG+CGG 0.424736 6.3:-1784091 None:intergenic
ATTTCCCTTGCCTGGGGAAG+AGG 0.433738 6.3:+1790227 MS.gene032574:CDS
AGTGTCCAAGCTGCCGTGTA+TGG 0.437138 6.3:+1790444 MS.gene032574:CDS
ACAGCACTTGCAAAAGCAAA+AGG 0.438572 6.3:+1786707 MS.gene032574:CDS
CAGAGAGTTGGCTTACAAAT+CGG 0.447484 6.3:+1787835 MS.gene032574:CDS
TTGGGCCAAGAAGTCCAATA+GGG 0.448894 6.3:+1789796 MS.gene032574:CDS
CAAGGACTATGTCTTTAGAT+TGG 0.454660 6.3:-1790137 None:intergenic
TCTGTTTCAAATAATGGAAA+GGG 0.454708 6.3:+1787668 MS.gene032574:CDS
GTGATTTCATGGAGGTGAGT+TGG 0.455330 6.3:+1783310 None:intergenic
CCTTATAATCTACGACTTCA+CGG 0.459640 6.3:-1790500 None:intergenic
CTGGTTTAGCAAGTCGAGAA+AGG 0.462717 6.3:-1787643 None:intergenic
AGATACACGAGTTGTTTGAT+TGG 0.464111 6.3:+1789777 MS.gene032574:CDS
TCGAGAAAACGTGCCAGACC+AGG 0.465385 6.3:-1787609 None:intergenic
AGACCAGGGGTATTTCCCTC+TGG 0.471013 6.3:-1787594 None:intergenic
ATTGAGCGATGATATCATAA+AGG 0.472275 6.3:+1790365 MS.gene032574:CDS
GCCCTTGCGACGAACCGTCC+TGG 0.475674 6.3:+1790159 MS.gene032574:CDS
ATCTCAGATGGCTAAATATG+AGG 0.478813 6.3:+1786405 MS.gene032574:CDS
CTAAATCACCCTTCTCAAGT+TGG 0.479623 6.3:-1786775 None:intergenic
AGGATGAGTTGGCAAGGAAG+AGG 0.479629 6.3:+1786425 MS.gene032574:CDS
CACCAGGACGGTTCGTCGCA+AGG 0.481861 6.3:-1790161 None:intergenic
CGCTTCCATACACGGCAGCT+TGG 0.482291 6.3:-1790449 None:intergenic
ACCCGCTTTCGAAGATGAAT+CGG 0.491673 6.3:-1784154 None:intergenic
TGTTGCTCCACTCGGATCAC+AGG 0.494047 6.3:+1789745 MS.gene032574:CDS
AAAATGAGTACCATAGAGTA+AGG 0.494834 6.3:+1786543 MS.gene032574:CDS
TTGTCTTCCCTGTTCCTGGA+GGG 0.498774 6.3:-1787805 None:intergenic
GGCTAAATATGAGGATGAGT+TGG 0.500126 6.3:+1786414 MS.gene032574:CDS
AGAGGGAAATACCCCTGGTC+TGG 0.500897 6.3:+1787596 MS.gene032574:CDS
TGAAAACACAAAATTACATC+AGG 0.504750 6.3:+1787754 MS.gene032574:CDS
AATGCCCTTCTCTTTCGTAC+TGG 0.505763 6.3:+1790111 MS.gene032574:CDS
CGCTCCTCTTCCCCAGGCAA+GGG 0.507955 6.3:-1790231 None:intergenic
GACGGTTCGTCGCAAGGGCA+AGG 0.508323 6.3:-1790155 None:intergenic
TGAAGAAGGAATGGCGCCAT+CGG 0.514941 6.3:-1784121 None:intergenic
GCAAGCTGAATTGGCTGCTA+AGG 0.515586 6.3:+1784502 MS.gene032574:CDS
ATTTCCCTCTGGAGGACTCT+CGG 0.515937 6.3:-1787583 None:intergenic
TGAGATATTGAAGAAACAAG+AGG 0.518331 6.3:+1784472 MS.gene032574:CDS
ATCTGTTTCAAATAATGGAA+AGG 0.518634 6.3:+1787667 MS.gene032574:CDS
AATCCAGAAGAAGCACTCCT+TGG 0.521894 6.3:-1784186 None:intergenic
CGATTTGTAAGCCAACTCTC+TGG 0.521968 6.3:-1787834 None:intergenic
GACAAGTACATAGCTCAAGC+TGG 0.523738 6.3:+1790279 MS.gene032574:CDS
TGGTTTAGCAAGTCGAGAAA+GGG 0.525545 6.3:-1787642 None:intergenic
ACGCTCCTCTTCCCCAGGCA+AGG 0.527101 6.3:-1790232 None:intergenic
TCTAACAGATCGTAATAAGT+TGG 0.527875 6.3:+1787223 MS.gene032574:CDS
GAGGACTCTCGGATCAATGA+TGG 0.530746 6.3:-1787572 None:intergenic
CTTGAGCTATGTACTTGTCT+AGG 0.530944 6.3:-1790275 None:intergenic
GGAGAAAGAGACGGTGGCGG+TGG 0.532338 6.3:-1784100 None:intergenic
ACCAGGACGGTTCGTCGCAA+GGG 0.533090 6.3:-1790160 None:intergenic
GAAGGTGCACGGGTTATTTG+GGG 0.534601 6.3:+1787524 MS.gene032574:CDS
ACCGATTCATCTTCGAAAGC+GGG 0.534856 6.3:+1784153 MS.gene032574:CDS
TTGGATTATGCATTGATGAC+TGG 0.537644 6.3:+1789716 MS.gene032574:CDS
CCTCCAGAGGGAAATACCCC+TGG 0.539942 6.3:+1787591 MS.gene032574:CDS
CTAATGTAGGGAAGGTGCAC+GGG 0.540661 6.3:+1787514 MS.gene032574:intron
AAGTTGGTCGTAACAGTTGG+TGG 0.542001 6.3:+1787239 MS.gene032574:CDS
ACTACCTTTGACCATATTGG+AGG 0.546392 6.3:+1786827 MS.gene032574:CDS
TTAAAACGCTCCTCTTCCCC+AGG 0.548339 6.3:-1790237 None:intergenic
GACAATGGCTGCCAGAGAGT+TGG 0.552771 6.3:+1787823 MS.gene032574:CDS
TAGTAAGAGAATTGAGCAGC+TGG 0.554062 6.3:+1787721 MS.gene032574:CDS
CCTAATGTAGGGAAGGTGCA+CGG 0.555109 6.3:+1787513 MS.gene032574:intron
CAGGAGAAACCAACTTGAGA+AGG 0.557237 6.3:+1786766 MS.gene032574:CDS
TCGGTTCCTGAAGATGAAGA+AGG 0.559401 6.3:-1784135 None:intergenic
ATTGTCTTCCCTGTTCCTGG+AGG 0.560179 6.3:-1787806 None:intergenic
TTCCCTTCCGGAAAACCCAT+CGG 0.562220 6.3:-1790403 None:intergenic
TCCTGAAGATGAAGAAGGAA+TGG 0.562260 6.3:-1784130 None:intergenic
GTTGGCAAGGAAGAGGATGC+AGG 0.565436 6.3:+1786432 MS.gene032574:CDS
AGAAGATTCATCAATCAGAC+TGG 0.565440 6.3:+1786589 MS.gene032574:CDS
GGAGGAGATGTTGCTCCACT+CGG 0.565757 6.3:+1789737 MS.gene032574:CDS
ATTCTAGGACCTCATCAATA+CGG 0.565931 6.3:-1790206 None:intergenic
GCCACCGTCTCTTTCTCCGA+TGG 0.566191 6.3:+1784105 MS.gene032574:CDS
AGTGACTGCTCTGGCAGCCG+GGG 0.566373 6.3:+1787262 MS.gene032574:CDS
GGTCTGCTACAGCTGAATCA+AGG 0.566819 6.3:-1790185 None:intergenic
TCAATTCTCTTACTAAGAGA+AGG 0.575379 6.3:-1787713 None:intergenic
TTTACCTCCAATATGGTCAA+AGG 0.577286 6.3:-1786831 None:intergenic
CAGCACTTGCAAAAGCAAAA+GGG 0.577360 6.3:+1786708 MS.gene032574:CDS
AGTTGGTGGAGTGACTGCTC+TGG 0.577512 6.3:+1787253 MS.gene032574:CDS
AATAAGTTGGTCGTAACAGT+TGG 0.580574 6.3:+1787236 MS.gene032574:CDS
AAAGCAAACTGTAGAGGAGA+AGG 0.582383 6.3:+1786655 MS.gene032574:CDS
ACAGTTGTCGCTTCCATACA+CGG 0.583469 6.3:-1790457 None:intergenic
CGAGAAAACGTGCCAGACCA+GGG 0.583981 6.3:-1787608 None:intergenic
GAAGCCGCAGCTAAAACCGA+TGG 0.596969 6.3:+1790387 MS.gene032574:CDS
TGAAGAAACAAGAGGAGACA+AGG 0.599789 6.3:+1784480 MS.gene032574:CDS
ATACCTTGTTGTGTAGACCC+CGG 0.606322 6.3:-1787279 None:intergenic
GGTCACCAGTACGAAAGAGA+AGG 0.606869 6.3:-1790116 None:intergenic
AGGAGAAACCAACTTGAGAA+GGG 0.608125 6.3:+1786767 MS.gene032574:CDS
GTCACCAGTACGAAAGAGAA+GGG 0.611997 6.3:-1790115 None:intergenic
GGCACAAACAAAATCTCAGA+TGG 0.613066 6.3:+1786393 MS.gene032574:CDS
AATATGAGGATGAGTTGGCA+AGG 0.615642 6.3:+1786419 MS.gene032574:CDS
AACCGATTCATCTTCGAAAG+CGG 0.617854 6.3:+1784152 MS.gene032574:CDS
CACGGAACAAGCTTGCGTCA+AGG 0.619585 6.3:-1790482 None:intergenic
CAGCCGGGGTCTACACAACA+AGG 0.619731 6.3:+1787276 MS.gene032574:CDS
CTACGAAGACGACATGGTGA+AGG 0.628592 6.3:+1783375 MS.gene032574:CDS
AAGCGGGTAACGATAATCCA+AGG 0.630177 6.3:+1784169 MS.gene032574:CDS
ATCGGAGAAAGAGACGGTGG+CGG 0.632706 6.3:-1784103 None:intergenic
AAGCCGCAGCTAAAACCGAT+GGG 0.633631 6.3:+1790388 MS.gene032574:CDS
TTTATGGCCCTCCAGGAACA+GGG 0.634037 6.3:+1787798 MS.gene032574:CDS
ACAGCTGAATCAAGGTCACC+AGG 0.637144 6.3:-1790177 None:intergenic
AAGGGAAATAGCGAAACTGA+TGG 0.642775 6.3:+1790419 MS.gene032574:CDS
CCTAGAATTTCCCTTGCCTG+GGG 0.646412 6.3:+1790221 MS.gene032574:CDS
GTTACAGCCTGTGATCCGAG+TGG 0.647718 6.3:-1789752 None:intergenic
GTGAAGCTACGAAGACGACA+TGG 0.648528 6.3:+1783369 MS.gene032574:CDS
CTGAATCAAGGTCACCAGGA+CGG 0.653067 6.3:-1790173 None:intergenic
ACTACGAAAGCAAACTGTAG+AGG 0.655152 6.3:+1786649 MS.gene032574:CDS
GGCGCCATCGGAGAAAGAGA+CGG 0.665134 6.3:-1784109 None:intergenic
TGTAGCAGACCGTATTGATG+AGG 0.665500 6.3:+1790197 MS.gene032574:CDS
TGGGCCAAGAAGTCCAATAG+GGG 0.667815 6.3:+1789797 MS.gene032574:CDS
GATTATGCATTGATGACTGG+AGG 0.668224 6.3:+1789719 MS.gene032574:CDS
TCCAGGAACAGGGAAGACAA+TGG 0.668408 6.3:+1787808 MS.gene032574:CDS
CCAGGGGTATTTCCCTCTGG+AGG 0.673340 6.3:-1787591 None:intergenic
TGATCCGAGAGTCCTCCAGA+GGG 0.678628 6.3:+1787579 MS.gene032574:CDS
TTGATCCGAGAGTCCTCCAG+AGG 0.688066 6.3:+1787578 MS.gene032574:CDS
GAGAAAACGTGCCAGACCAG+GGG 0.697208 6.3:-1787607 None:intergenic
GCCATCGGAGAAAGAGACGG+TGG 0.704426 6.3:-1784106 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr6.3 gene 1783318 1790572 1783318 ID=MS.gene032574
chr6.3 mRNA 1783318 1790572 1783318 ID=MS.gene032574.t1;Parent=MS.gene032574
chr6.3 exon 1783318 1783396 1783318 ID=MS.gene032574.t1.exon1;Parent=MS.gene032574.t1
chr6.3 CDS 1783318 1783396 1783318 ID=cds.MS.gene032574.t1;Parent=MS.gene032574.t1
chr6.3 exon 1784085 1784278 1784085 ID=MS.gene032574.t1.exon2;Parent=MS.gene032574.t1
chr6.3 CDS 1784085 1784278 1784085 ID=cds.MS.gene032574.t1;Parent=MS.gene032574.t1
chr6.3 exon 1784467 1784562 1784467 ID=MS.gene032574.t1.exon3;Parent=MS.gene032574.t1
chr6.3 CDS 1784467 1784562 1784467 ID=cds.MS.gene032574.t1;Parent=MS.gene032574.t1
chr6.3 exon 1786343 1786453 1786343 ID=MS.gene032574.t1.exon4;Parent=MS.gene032574.t1
chr6.3 CDS 1786343 1786453 1786343 ID=cds.MS.gene032574.t1;Parent=MS.gene032574.t1
chr6.3 exon 1786539 1786848 1786539 ID=MS.gene032574.t1.exon5;Parent=MS.gene032574.t1
chr6.3 CDS 1786539 1786848 1786539 ID=cds.MS.gene032574.t1;Parent=MS.gene032574.t1
chr6.3 exon 1787207 1787297 1787207 ID=MS.gene032574.t1.exon6;Parent=MS.gene032574.t1
chr6.3 CDS 1787207 1787297 1787207 ID=cds.MS.gene032574.t1;Parent=MS.gene032574.t1
chr6.3 exon 1787523 1787856 1787523 ID=MS.gene032574.t1.exon7;Parent=MS.gene032574.t1
chr6.3 CDS 1787523 1787856 1787523 ID=cds.MS.gene032574.t1;Parent=MS.gene032574.t1
chr6.3 exon 1789713 1789861 1789713 ID=MS.gene032574.t1.exon8;Parent=MS.gene032574.t1
chr6.3 CDS 1789713 1789861 1789713 ID=cds.MS.gene032574.t1;Parent=MS.gene032574.t1
chr6.3 exon 1790068 1790572 1790068 ID=MS.gene032574.t1.exon9;Parent=MS.gene032574.t1
chr6.3 CDS 1790068 1790572 1790068 ID=cds.MS.gene032574.t1;Parent=MS.gene032574.t1
Gene Sequence

>MS.gene032574

ATGGAGGTGAGTTGGGAATTACATCGATTTTGGGTTGAAATCAGGTTAAGGGTGAAGCTACGAAGACGACATGGTGAAGGTGATTGGAAGGCAGAAAAAACCGATTGAATAGCACGATTAAAACGGATGAAGAGAACGCTATACACCATAGCTCCCTAAAACCCTAGCAGAGGGTGGGAGCGGCGCAAGGATTCAATCCCAAAAAAAAAAAAAAACTAAAGAGGTTGAGATCGCTTAATAATAACTTATCATTTTTCTTTCAATTTTAACCCCTATCATCTTATATGAAACAAATTAAAATAATTAAACATACATCATTTTACATTTATAAGTCGATTTAACAACAAATTTGATCAAACATAATAAATTCAACGAGCTAGTTCATACACTATAAGCTAGCTTAAGCTATTAGCTAAAAACTCGTCCTTTATAAGTTAGTTTATCATCTATCCCCTACTTTTTTTTATCAAAAGGAGTCATAAGATCACTCATAAGCCATACTAAAAAATTAAAATTATAACGAGTTAAGCAGAGTCGACCCAATAATTTTTGGAAAAAATTAAATCTAATAGTAACTTGTTGAATAAAAAAGAGAAGAATCTATCTAACTTGTTGGAAAAGTGAAATTCGGGTCAAATCAAAGCTCCGAATAAAAAACCCATTTCGTTTTCGGAACCCTACCAAAACCCTCAGAACCTTTTCTTCACCATTCTTCACAAAAACCTCTTCTTCAATGGCGAAAACCACTTTATCCGGTTTATTCTCAGCCTTAACCGCCGCCACCACCGCCACCGTCTCTTTCTCCGATGGCGCCATTCCTTCTTCATCTTCAGGAACCGATTCATCTTCGAAAGCGGGTAACGATAATCCAAGGAGTGCTTCTTCTGGATTCGATCCTGAACCTCTTGAAAAAGGTCTTAAAGCTCTCAAACAAATCTCTGCTTCGTCATATGCTAAAAAGGTATCGTATCTCAAAACACTGACACACGACATTGACACGTCTGCGTCAGTTATAATCTGAGAAACTAACATAATTGAATGTAATCACATGTGTTAGTGTCTACACAACTTCTATTCTATTCAATTATTCTTTTGCATTATGTTTAATATTTTATTTGTGGTTTTTGTTTTTGTTTTTTTTTTTTGAAGGTGTTTGAGATATTGAAGAAACAAGAGGAGACAAGGCAAGCTGAATTGGCTGCTAAGGTTGCTGAGTTTAATCAAATGAAAGCACAATTGGAGACTGTAAGTTTTGTCCAGAAATTCTTATGTTGTCAGTTTCGTAAGTAGAACTCAAATTGCGGAGACATTTGATATGTCGAGGCAAAGATTGTGTGTAAAATAGCATTTTTGTATGCGAAATAGGTTTTTTTTGTCTGAGTAATGATATTTGTACAACTATTTTCTGACAACTTTCTTTTTATTGGTCGAAAACAATAGAGAGAGAGAGAGAGTAAGAACATGACGAGAGTATGAGAGACAGTTATCCGAAAAATGGCTGTAAAATTGTATGCTGTCAGCTTCATAAGTAGAACTCAAAAGATGGTAAATGACTGCAGAGACAATTGATATGCTGGATTGCGAATTTAATGTAAACTTGCATTTTAGTAGTGTGTATAAGTTGAGCTGTTTGAAACAAGAATTTTTTTAGTAGTAATTGCATGTTATCGGTCCCGTAATTAAAACTCAGATGATAAAAGATTGCAGAGACGATTAATATGATCAGTGGCAGATTTCATGTAAAATTGCATTTTAGTAGTGTGCGTGTGAGTTTCGGCCTTCGGGGCTTGGCTCTTTGAAACCAAATAAATTTGCATGTTATTATTTTTTTTAATTTGTTTTACATTATTATTATTTCTACTCTATTTAGTATTCATTATTATACACATGTTTTCTTGGGAATGTTCTTTTTTAAACAAGTTTTTTTTTTTTTTTTACAATAATGAAGTCTGTTTCAGAGAAGATAGAATTTGAGAGACAAATTATGTAGTTTTGTAGCTTGAAGGTTTGTGTGGGTGTCCGACATGTTTTGGACACTCAAACACTTGTACACCCGACACAGAAAAGCATACACCGTTGATAATTTGAGAAAATAGAATTGATTAAATGTAACATCTGTTGGTGTCCGACTCATGCCATTGTCGGACACCAGACACACCTTCAATATGAAGTGCTAATGCTACATTGCATCTGACACTCCATTTCATTGTCTGAAAATATATATATTTTTTTGATTATTTTTTCCTCAGCGTGTTGAAGACGGGGCTAAAATTGATTTGATATTTTTTTTATATATATTTTCGGTATTAGATGGATGGAACATGAAAGACCTTGAATAGTTATCATGAATGAATGGTGTCTCTCAGTACTTTACATGAGTCTGTATCATGGGGAAAATGTAATAATTCTCGACAAATTCTCGTTTGAATTTTATCCCATTGGGGTGTATGCGTTTCATCTCCCATAATTAGGACATTACTGTCTAGGATTTCTGTGTAAGTTTTTTTTGGTTACTGACAAATGTCCGTATTTTCAAACTCTGGACTTTTAACTCCTTAATTCCTTCAAACCCTGGACTGTAAGTGCTTCATAGCAGACAGGAGTTTTTTTGTTTGTATCAATTACTAACAACTTCAAAGTATTCGTAGGAACAGAAGACTAACTCTATCTGGAAGACTCTAGAACAATAATAACAATGTTTCAGGGGTTACACCTTTCCATACTGCCTATCAAATTTTTATTATTGTAAGTTGAAATTGTCTACAACAACAACAACCAAGCCTTTTCCCACTAAGTGGGGTTGGCTACCTTGATCAAATTACGCCATAATGTTCTATCAGTTTAATAAATCATATGCATTGTTGTGTAAGTTCTAGCTTCATGATGAAATGAGTTTATTTTATTGCAAGTACAAGTATGGCCTTAAACAATTGAAGATATGTGTGAAAGTGAAACTTTCTTTGCTCCTGTTCTGTGTGTATTTTGTGTTTTGGTTTTCCAGCTTATCAGTTGGTTTTCTTGGCAGGAAAAACAAAGGATTGTATATGACGAAGAAAAAAAGCTGGCCCAACATCAGGCACAAACAAAATCTCAGATGGCTAAATATGAGGATGAGTTGGCAAGGAAGAGGATGCAGGTATTACATTAAACCTTTTGAAACTTTGGGTTTTTGTTTTTGTCAATTTTCAAGTCTTTTCTGATTCTCACATTTTTATTGTCAGGCAGAAAATGAGTACCATAGAGTAAGGAACCAAGAGCTTGTAAAATTGCAAGAAGATTCATCAATCAGACTGGAGCAAACTAGACTTGAAACAGAAAAACACATTCAAGCACTACGAAAGCAAACTGTAGAGGAGAAGGCTAAGCTTGAACATGACACAATCAGAGAAACAGCACTTGCAAAAGCAAAAGGGAAAGTGCTCGAAATAACGAAAAACGAAGAGATTGACAGGAGAAACCAACTTGAGAAGGGTGATTTAGATCGAGAAAAATGGATTTCTATCATAAATACTACCTTTGACCATATTGGAGGTAAATTTTTTACCAGATACGAACTATATATTATGCAATTAGTATTTGTTGATTTGAATGCACAAACTAAAGCAATTCTTTATATACTCTTTTGCCTTTAAAAACGAATTCACGATGATTTTTGTGGTAATGCTATTTTGTCAATTTTACTTTCATCTTGTTGTTTAATCATTAGTCAGCATAATTGTAGTTTGTTGGTTGTATTATTGTTTAATTTTTTTATCGTTATTTATTGTTTCTAATTACGTGAAGCATTGTATCTTAATTGGATGATGAACTCAATCAATATTTTTGCTTGGCTGGGCTAGACAAACTCCACACCAATCCCCAAATCTGAAAACTCAATTATCTTTTGCAGGGGGCTTTAAAGCCATTCTAACAGATCGTAATAAGTTGGTCGTAACAGTTGGTGGAGTGACTGCTCTGGCAGCCGGGGTCTACACAACAAGGTATAAATTGTCCATAAAGTTTGGAATGAGCTACTATAAACGAGGAAGGAGGGAAAAAATTGGTTGAGTGTTATTTTTGCATGGCTAGCCCCATTTTTAGAGAATATACAACTTTAAGTTTATGCCCTCTATCTATCAGACTATTTCTTATCTCAGTAGTGAAACTTTACATTGTTATTTGCATGCTGCTATGTATAATTTACATTCAGATTGTACCTAATGTAGGGAAGGTGCACGGGTTATTTGGGGATATGTTGATAGAATATTGGGACAACCATCATTGATCCGAGAGTCCTCCAGAGGGAAATACCCCTGGTCTGGCACGTTTTCTCGAGCCAAAAGCACCCTTTCTCGACTTGCTAAACCAGAATCTGTTTCAAATAATGGAAAGGGTTTTGGCGATGTAATTTTGCATCCTTCTCTTAGTAAGAGAATTGAGCAGCTGGCGTTTGCAACTGAAAACACAAAATTACATCAGGCACCATTCAGAAACGTGCTTTTTTATGGCCCTCCAGGAACAGGGAAGACAATGGCTGCCAGAGAGTTGGCTTACAAATCGGTACGACTTTGTACTTATCCATTTATCTTCTTGTTCTAATTTGATGTTTTTCAATGTCCAAAAAAAATATGTTTCATGTTATTTACCTTGTTTTGTCAACGTGTCAAATAAGTAGGGAATTGGTTGTATGCATTGTTTATGCTATCCTATATTAAGTAGAAGTGATCAACATATTAGGAAAAATGTTTCCTCATTTTTCAGATTTTTTATATATTCCTCTTTATAATTTCTGGCTGACAGTCTAGGGTGAACGATCTACAAATTAATGAATCTAAAATCATCTTTCTTAAAGGCTCTGCAAATTCATTTCCCAATTGTCTTTCTAAATTCAATGCCAAGTTCTTTTTCTGTGGAATTTTGCAGTTATTAGCTTACAAGTGGGTATTCAGTACCATGCTTCTGGTTATTGCTGTTTTATGAAGGTGTAATAATCACTTTGGTCCCTGAATGTGTAGCAAGTAGTCACAATAGTCCTTCAATGTATCAAAATTCCAAAATAGTCCCTGATTGTCCACTTTGTTCGTCAAAATAGTCCCTAAAGCTTAAATAACCCCTTAATCTCTGAAGCCCCGATACTCCAAAAATGGTGAAGTATCGTATCCGACACATATCCGATACCGATACGCCCCGATACGCGTATCGGAAAAGTATCGGGCAATTAATATTAATTTTTTTGAAAAACAATGACCGATACGCGTCGGATACGTCCCGATACGCGTATCGGAGAAGTATCGGGCAGTTAATATTTATTTTTTGAAGGAAAAAAATGACCGGGGATACGACTAATTTGTACTCCGACACAGCTTACTTATACTCCGACACCTATACTTCTGTCTTACATATTTCCTTTAAAAAAAAGTAAAATAAAATAATAAGAAATAAAACTACAAGTCTGGCAGCGCTATCAAAATCATATTGTCTCTTCTAACCTAAAAAAATAGGGTTTCTCGTCACCACATAGCAATAGCTATTCTCTCTTCTAACCATTACCACTGATTGATTCTCTCTAATGTTACGTTTCTTCTGCTGTTTGTTTTCGATGTAAATTAGTGTTAACTGTTAAGTAGTCAACATTAGACAAAGATGTTCTTTTTTTTATAGTACTTATGATGTTCTCTTTGGATGGTACTAATGATGTTTAGCTATATTCTATCTAATTTGTGCGGAAAAAATATGTAATTGTCACTTGTTTTGATCATTTTCATTAATGTGAATGTTTGTTTATTATCATTTTTAGTTATGTTTATACATTGTGCACTTATACTATTAATTTTAAATTTAATTTTTTAATAACGTATCGAGGCCGTATCGTATCGGGAATTTTGAAATTTTTCCCGTATCGCCGTATCGGTATCGTATCGTATCGGTATCGTGTCAGGTATCGGGGCTTCATAGCCCTTAATACTCACAATAACTCTTTTGATATAAGGACTAAAATGACAATAAGTAAGCACGTATAAGGACTAATATGACAATAAGTAAGTCTATTGAAGGACTAATATGACAATATTAACGAAATATTTTAATATCAAGGACTATTTTGACTAACAAAGTGCACAATCAGGGACTATTTTGCTATTTCGATACATTGAGGGACTATTATGACTACTCGTCACTCATTCAAGGACCAAAGTGACTATTTCCCCGTTTTATGAATTGCATCTTTTTTTTTTTTTTGAATGGAAAATATATATTATATATTAAATATAAAAAGGAGAAATCTCCTACAAGAGAAATCTCCTTGAAGTGTATCTTGAACATTTGACCTTATTTGCTCATCATTTTTTTAACTGATATTCTTTCCTTCTAGTTAAAATGATATTTTGTCTGAATTTTTGTTTTCAATTTAAATTCAGGGATTGGATTATGCATTGATGACTGGAGGAGATGTTGCTCCACTCGGATCACAGGCTGTAACAAAGATACACGAGTTGTTTGATTGGGCCAAGAAGTCCAATAGGGGTTTACTGCTTTTTATTGACGAAGCTGATGCATTTCTATGCGAGTAAGCAATGCATCTACCATTCCATGCTTTTCATATATTATTATCCGACTCGGTTCTTGTGGCATCATAAGATGATTATGATCAGCCAGATGCAATTTCATTACTTTTTTTAAGAAGCAAAGTTCCAAAAAGTGAGTAAAAGTTGCAATGTTAATGAAACTAACATTAGTTGAGAATTGTCTGAGAAAATTGGGTGAATTTTGCAGGCGGAACAAGACGTATATGAGTGAAGCACAAAGAAGTGCACTAAATGCCCTTCTCTTTCGTACTGGTGACCAATCTAAAGACATAGTCCTTGCCCTTGCGACGAACCGTCCTGGTGACCTTGATTCAGCTGTAGCAGACCGTATTGATGAGGTCCTAGAATTTCCCTTGCCTGGGGAAGAGGAGCGTTTTAAACTTCTTAAGCTCTACCTAGACAAGTACATAGCTCAAGCTGGATCAAGAAAATCTGGTTTATTATCATTGTTCAAAGCAAGCCCACAAAAGATAGAGATTAAAGGATTGAGCGATGATATCATAAAGGAAGCCGCAGCTAAAACCGATGGGTTTTCCGGAAGGGAAATAGCGAAACTGATGGCAAGTGTCCAAGCTGCCGTGTATGGAAGCGACAACTGTATCCTTGACGCAAGCTTGTTCCGTGAAGTCGTAGATTATAAGGTTGCTGAGCATCAACAAAGAATAAAATTGGCAGCTTCTGATAAAGCTTAG
Protein sequence

>MS.gene032574.t1

MEVSWELHRFWVEIRLRVKLRRRHGEALTAATTATVSFSDGAIPSSSSGTDSSSKAGNDNPRSASSGFDPEPLEKGLKALKQISASSYAKKVFEILKKQEETRQAELAAKVAEFNQMKAQLETEKQRIVYDEEKKLAQHQAQTKSQMAKYEDELARKRMQAENEYHRVRNQELVKLQEDSSIRLEQTRLETEKHIQALRKQTVEEKAKLEHDTIRETALAKAKGKVLEITKNEEIDRRNQLEKGDLDREKWISIINTTFDHIGGGFKAILTDRNKLVVTVGGVTALAAGVYTTREGARVIWGYVDRILGQPSLIRESSRGKYPWSGTFSRAKSTLSRLAKPESVSNNGKGFGDVILHPSLSKRIEQLAFATENTKLHQAPFRNVLFYGPPGTGKTMAARELAYKSGLDYALMTGGDVAPLGSQAVTKIHELFDWAKKSNRGLLLFIDEADAFLCERNKTYMSEAQRSALNALLFRTGDQSKDIVLALATNRPGDLDSAVADRIDEVLEFPLPGEEERFKLLKLYLDKYIAQAGSRKSGLLSLFKASPQKIEIKGLSDDIIKEAAAKTDGFSGREIAKLMASVQAAVYGSDNCILDASLFREVVDYKVAEHQQRIKLAASDKA