Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034241.t1 | XP_003604244.1 | 100 | 259 | 0 | 0 | 1 | 259 | 1 | 259 | 5.00E-133 | 483.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034241.t1 | Q9XGM1 | 75.2 | 262 | 61 | 2 | 1 | 259 | 1 | 261 | 7.4e-101 | 368.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034241.t1 | G7JEF1 | 100.0 | 259 | 0 | 0 | 1 | 259 | 1 | 259 | 3.6e-133 | 483.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene34307 | MS.gene034241 | PPI |
| MS.gene024260 | MS.gene034241 | PPI |
| MS.gene034241 | MS.gene87386 | PPI |
| MS.gene28505 | MS.gene034241 | PPI |
| MS.gene54962 | MS.gene034241 | PPI |
| MS.gene44998 | MS.gene034241 | PPI |
| MS.gene034241 | MS.gene98140 | PPI |
| MS.gene034241 | MS.gene011917 | PPI |
| MS.gene72597 | MS.gene034241 | PPI |
| MS.gene034241 | MS.gene034283 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034241.t1 | MTR_4g007030 | 100.000 | 259 | 0 | 0 | 1 | 259 | 1 | 259 | 0.0 | 524 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034241.t1 | AT3G58730 | 79.389 | 262 | 50 | 2 | 1 | 259 | 1 | 261 | 1.47e-152 | 426 |
Find 61 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTAGGTTGAAGAAGATTC+AGG | 0.251278 | 4.1:+88388175 | MS.gene034241:CDS |
| GAATACTATTAGTTACATTA+AGG | 0.278798 | 4.1:+88388121 | MS.gene034241:CDS |
| GTGTCGTGTTGCTTATATTA+AGG | 0.324047 | 4.1:+88387977 | MS.gene034241:CDS |
| TTGAAAGGGAGGATTTCTTT+AGG | 0.370172 | 4.1:+88388159 | MS.gene034241:CDS |
| AACTTGTTGGCTGGTACGAC+TGG | 0.385007 | 4.1:+88388302 | MS.gene034241:CDS |
| GACAATGCTTGGAGTGGTTA+AGG | 0.388176 | 4.1:+88387611 | MS.gene034241:CDS |
| TGAAACCTCTCACAACTTGT+TGG | 0.400530 | 4.1:+88388289 | MS.gene034241:CDS |
| AGAAGATTCAGGGTTATAAG+AGG | 0.430053 | 4.1:+88388186 | MS.gene034241:CDS |
| TGTTTCAACAAAGGAATCAA+TGG | 0.435503 | 4.1:+88387725 | MS.gene034241:CDS |
| GTGAGTTGGATGAGCTTGAA+AGG | 0.439414 | 4.1:+88388144 | MS.gene034241:CDS |
| AATACTATTAGTTACATTAA+GGG | 0.450457 | 4.1:+88388122 | MS.gene034241:CDS |
| TAGTTACATTAAGGGTGAGT+TGG | 0.451344 | 4.1:+88388130 | MS.gene034241:CDS |
| AAAGGAAGATGGTGATTGCT+AGG | 0.464882 | 4.1:+88388219 | MS.gene034241:CDS |
| TGGAGTGGTTAAGGCACGTT+TGG | 0.467712 | 4.1:+88387620 | MS.gene034241:CDS |
| GTTTCAACAAAGGAATCAAT+GGG | 0.471657 | 4.1:+88387726 | MS.gene034241:CDS |
| ACCTCTCACAACTTGTTGGC+TGG | 0.473238 | 4.1:+88388293 | MS.gene034241:CDS |
| TGCTTATATTAAGGCGATTG+AGG | 0.474951 | 4.1:+88387986 | MS.gene034241:CDS |
| AAAAGTTACAATTGCAGCAA+GGG | 0.488148 | 4.1:+88388258 | MS.gene034241:CDS |
| CTAATAGTATTCTCCAACCT+CGG | 0.495607 | 4.1:-88388110 | None:intergenic |
| GTTGGATGAGCTTGAAAGGG+AGG | 0.499311 | 4.1:+88388148 | MS.gene034241:CDS |
| GTGGTTAAGGCACGTTTGGT+TGG | 0.501885 | 4.1:+88387624 | MS.gene034241:CDS |
| TTTAGGTTGAAGAAGATTCA+GGG | 0.502528 | 4.1:+88388176 | MS.gene034241:CDS |
| TAATAGCATCATCAAGGGTA+AGG | 0.507164 | 4.1:-88388043 | None:intergenic |
| TGAGTTGGATGAGCTTGAAA+GGG | 0.514528 | 4.1:+88388145 | MS.gene034241:CDS |
| ATTGGCTAGAGGTGGTCAGC+AGG | 0.515670 | 4.1:+88387947 | MS.gene034241:CDS |
| GATTCAGGGTTATAAGAGGA+GGG | 0.517039 | 4.1:+88388190 | MS.gene034241:CDS |
| TCAGCACTGTAATCAAACTT+TGG | 0.538953 | 4.1:-88387894 | None:intergenic |
| TAGTCTTAATAGCATCATCA+AGG | 0.542669 | 4.1:-88388049 | None:intergenic |
| GAAAAGTTACAATTGCAGCA+AGG | 0.543936 | 4.1:+88388257 | MS.gene034241:CDS |
| AGATTCAGGGTTATAAGAGG+AGG | 0.545100 | 4.1:+88388189 | MS.gene034241:CDS |
| GAGGGAAATTGAAAGGAAGA+TGG | 0.546430 | 4.1:+88388208 | MS.gene034241:CDS |
| GAATGTTGTTAAGCCGAGGT+TGG | 0.546577 | 4.1:+88388097 | MS.gene034241:CDS |
| GTCCCCACAGTGACAATGCT+TGG | 0.555756 | 4.1:+88387600 | MS.gene034241:CDS |
| ATCATCAAGGGTAAGGAAAG+AGG | 0.556678 | 4.1:-88388036 | None:intergenic |
| ATAAGAGGAGGGAAATTGAA+AGG | 0.563654 | 4.1:+88388201 | MS.gene034241:CDS |
| TCTCGTACTGAGAATGTGGC+AGG | 0.566710 | 4.1:+88387861 | MS.gene034241:CDS |
| ATGTTAAAGAAGCTTCACTC+AGG | 0.568588 | 4.1:+88387832 | MS.gene034241:CDS |
| CTATTAAGACTACTAACCGT+AGG | 0.570720 | 4.1:+88388060 | MS.gene034241:CDS |
| ACTCCAAGCATTGTCACTGT+GGG | 0.583853 | 4.1:-88387603 | None:intergenic |
| ACTGAGGCTAAGTATGTAGC+CGG | 0.591330 | 4.1:+88387780 | MS.gene034241:CDS |
| TTCTTCATTTGCATTAACTG+AGG | 0.593026 | 4.1:+88387764 | MS.gene034241:CDS |
| CGTTTGGTTGGAGCAACAAG+AGG | 0.602491 | 4.1:+88387636 | MS.gene034241:CDS |
| TTGAGAATGTTGTTAAGCCG+AGG | 0.609957 | 4.1:+88388093 | MS.gene034241:CDS |
| AAGGAAGATGGTGATTGCTA+GGG | 0.610095 | 4.1:+88388220 | MS.gene034241:CDS |
| CACTCCAAGCATTGTCACTG+TGG | 0.610571 | 4.1:-88387604 | None:intergenic |
| AAGTTTGATTACAGTGCTGA+CGG | 0.611337 | 4.1:+88387897 | MS.gene034241:CDS |
| TCTCAAGAGCATTAACCCTA+CGG | 0.615251 | 4.1:-88388076 | None:intergenic |
| AAAGTTACAATTGCAGCAAG+GGG | 0.616721 | 4.1:+88388259 | MS.gene034241:CDS |
| AGTCTTAATAGCATCATCAA+GGG | 0.622324 | 4.1:-88388048 | None:intergenic |
| GACGTGCTTGATGTTATCAC+CGG | 0.624967 | 4.1:-88387799 | None:intergenic |
| ACGTTCTCGTACTGAGAATG+TGG | 0.627068 | 4.1:+88387857 | MS.gene034241:CDS |
| CACAGTGACAATGCTTGGAG+TGG | 0.627138 | 4.1:+88387605 | MS.gene034241:CDS |
| TATTAAGACTACTAACCGTA+GGG | 0.628796 | 4.1:+88388061 | MS.gene034241:CDS |
| ACCAGCCAACAAGTTGTGAG+AGG | 0.637543 | 4.1:-88388294 | None:intergenic |
| GCAATCACAACGTTTAAATG+TGG | 0.644830 | 4.1:+88387578 | MS.gene034241:CDS |
| TGTTAAAGAAGCTTCACTCA+GGG | 0.649586 | 4.1:+88387833 | MS.gene034241:CDS |
| CTGACTGGATTGGCTAGAGG+TGG | 0.655401 | 4.1:+88387939 | MS.gene034241:CDS |
| TGATTACAGTGCTGACGGTG+AGG | 0.656119 | 4.1:+88387902 | MS.gene034241:CDS |
| GATCTGACTGGATTGGCTAG+AGG | 0.672119 | 4.1:+88387936 | MS.gene034241:CDS |
| CTCCAAGCATTGTCACTGTG+GGG | 0.709998 | 4.1:-88387602 | None:intergenic |
| TGGTACGACTGGAAAAGACG+AGG | 0.748180 | 4.1:+88388313 | MS.gene034241:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATACTATTAGTTACATTAA+GGG | + | chr4.1:88388122-88388141 | MS.gene034241:CDS | 15.0% |
| !! | GAATACTATTAGTTACATTA+AGG | + | chr4.1:88388121-88388140 | MS.gene034241:CDS | 20.0% |
| ! | GAAAAAGATTGTTTCAACAA+AGG | + | chr4.1:88387716-88387735 | MS.gene034241:CDS | 25.0% |
| AAAAGTTACAATTGCAGCAA+GGG | + | chr4.1:88388258-88388277 | MS.gene034241:CDS | 30.0% | |
| AGTCTTAATAGCATCATCAA+GGG | - | chr4.1:88388051-88388070 | None:intergenic | 30.0% | |
| GTTTCAACAAAGGAATCAAT+GGG | + | chr4.1:88387726-88387745 | MS.gene034241:CDS | 30.0% | |
| TATTAAGACTACTAACCGTA+GGG | + | chr4.1:88388061-88388080 | MS.gene034241:CDS | 30.0% | |
| TGTTTCAACAAAGGAATCAA+TGG | + | chr4.1:88387725-88387744 | MS.gene034241:CDS | 30.0% | |
| TTCTTCATTTGCATTAACTG+AGG | + | chr4.1:88387764-88387783 | MS.gene034241:CDS | 30.0% | |
| ! | TAGTCTTAATAGCATCATCA+AGG | - | chr4.1:88388052-88388071 | None:intergenic | 30.0% |
| !! | TTTAGGTTGAAGAAGATTCA+GGG | + | chr4.1:88388176-88388195 | MS.gene034241:CDS | 30.0% |
| AAAGTTACAATTGCAGCAAG+GGG | + | chr4.1:88388259-88388278 | MS.gene034241:CDS | 35.0% | |
| AGAAGATTCAGGGTTATAAG+AGG | + | chr4.1:88388186-88388205 | MS.gene034241:CDS | 35.0% | |
| ATAAGAGGAGGGAAATTGAA+AGG | + | chr4.1:88388201-88388220 | MS.gene034241:CDS | 35.0% | |
| ATGTTAAAGAAGCTTCACTC+AGG | + | chr4.1:88387832-88387851 | MS.gene034241:CDS | 35.0% | |
| CTAATAGTATTCTCCAACCT+CGG | - | chr4.1:88388113-88388132 | None:intergenic | 35.0% | |
| CTATTAAGACTACTAACCGT+AGG | + | chr4.1:88388060-88388079 | MS.gene034241:CDS | 35.0% | |
| GAAAAATGATCTGACTGGAT+TGG | + | chr4.1:88387929-88387948 | MS.gene034241:CDS | 35.0% | |
| GAAAAGTTACAATTGCAGCA+AGG | + | chr4.1:88388257-88388276 | MS.gene034241:CDS | 35.0% | |
| GCAATCACAACGTTTAAATG+TGG | + | chr4.1:88387578-88387597 | MS.gene034241:CDS | 35.0% | |
| TAATAGCATCATCAAGGGTA+AGG | - | chr4.1:88388046-88388065 | None:intergenic | 35.0% | |
| TAGTTACATTAAGGGTGAGT+TGG | + | chr4.1:88388130-88388149 | MS.gene034241:CDS | 35.0% | |
| TCAGCACTGTAATCAAACTT+TGG | - | chr4.1:88387897-88387916 | None:intergenic | 35.0% | |
| TGCTTATATTAAGGCGATTG+AGG | + | chr4.1:88387986-88388005 | MS.gene034241:CDS | 35.0% | |
| TGTTAAAGAAGCTTCACTCA+GGG | + | chr4.1:88387833-88387852 | MS.gene034241:CDS | 35.0% | |
| TTGAAAGGGAGGATTTCTTT+AGG | + | chr4.1:88388159-88388178 | MS.gene034241:CDS | 35.0% | |
| ! | AAGTTTGATTACAGTGCTGA+CGG | + | chr4.1:88387897-88387916 | MS.gene034241:CDS | 35.0% |
| !! | CTTTAGGTTGAAGAAGATTC+AGG | + | chr4.1:88388175-88388194 | MS.gene034241:CDS | 35.0% |
| !! | GTGTCGTGTTGCTTATATTA+AGG | + | chr4.1:88387977-88387996 | MS.gene034241:CDS | 35.0% |
| AAAGGAAGATGGTGATTGCT+AGG | + | chr4.1:88388219-88388238 | MS.gene034241:CDS | 40.0% | |
| AAGGAAGATGGTGATTGCTA+GGG | + | chr4.1:88388220-88388239 | MS.gene034241:CDS | 40.0% | |
| AGATTCAGGGTTATAAGAGG+AGG | + | chr4.1:88388189-88388208 | MS.gene034241:CDS | 40.0% | |
| ATCATCAAGGGTAAGGAAAG+AGG | - | chr4.1:88388039-88388058 | None:intergenic | 40.0% | |
| GAGGGAAATTGAAAGGAAGA+TGG | + | chr4.1:88388208-88388227 | MS.gene034241:CDS | 40.0% | |
| GATTCAGGGTTATAAGAGGA+GGG | + | chr4.1:88388190-88388209 | MS.gene034241:CDS | 40.0% | |
| TGAAACCTCTCACAACTTGT+TGG | + | chr4.1:88388289-88388308 | MS.gene034241:CDS | 40.0% | |
| TTGAGAATGTTGTTAAGCCG+AGG | + | chr4.1:88388093-88388112 | MS.gene034241:CDS | 40.0% | |
| ! | TGAGTTGGATGAGCTTGAAA+GGG | + | chr4.1:88388145-88388164 | MS.gene034241:CDS | 40.0% |
| !! | TCCAGTCAGATCATTTTTCG+TGG | - | chr4.1:88387928-88387947 | None:intergenic | 40.0% |
| !! | TCTCAAGAGCATTAACCCTA+CGG | - | chr4.1:88388079-88388098 | None:intergenic | 40.0% |
| ACGTTCTCGTACTGAGAATG+TGG | + | chr4.1:88387857-88387876 | MS.gene034241:CDS | 45.0% | |
| ACTGAGGCTAAGTATGTAGC+CGG | + | chr4.1:88387780-88387799 | MS.gene034241:CDS | 45.0% | |
| GAATGTTGTTAAGCCGAGGT+TGG | + | chr4.1:88388097-88388116 | MS.gene034241:CDS | 45.0% | |
| GACAATGCTTGGAGTGGTTA+AGG | + | chr4.1:88387611-88387630 | MS.gene034241:CDS | 45.0% | |
| GACGTGCTTGATGTTATCAC+CGG | - | chr4.1:88387802-88387821 | None:intergenic | 45.0% | |
| GCCACGAAAAATGATCTGAC+TGG | + | chr4.1:88387924-88387943 | MS.gene034241:CDS | 45.0% | |
| ! | ACTCCAAGCATTGTCACTGT+GGG | - | chr4.1:88387606-88387625 | None:intergenic | 45.0% |
| ! | GTGAGTTGGATGAGCTTGAA+AGG | + | chr4.1:88388144-88388163 | MS.gene034241:CDS | 45.0% |
| ACCAGCCAACAAGTTGTGAG+AGG | - | chr4.1:88388297-88388316 | None:intergenic | 50.0% | |
| CACAGTGACAATGCTTGGAG+TGG | + | chr4.1:88387605-88387624 | MS.gene034241:CDS | 50.0% | |
| CGTTTGGTTGGAGCAACAAG+AGG | + | chr4.1:88387636-88387655 | MS.gene034241:CDS | 50.0% | |
| GTGGTTAAGGCACGTTTGGT+TGG | + | chr4.1:88387624-88387643 | MS.gene034241:CDS | 50.0% | |
| GTTGGATGAGCTTGAAAGGG+AGG | + | chr4.1:88388148-88388167 | MS.gene034241:CDS | 50.0% | |
| TCTCGTACTGAGAATGTGGC+AGG | + | chr4.1:88387861-88387880 | MS.gene034241:CDS | 50.0% | |
| TGGAGTGGTTAAGGCACGTT+TGG | + | chr4.1:88387620-88387639 | MS.gene034241:CDS | 50.0% | |
| TGGTACGACTGGAAAAGACG+AGG | + | chr4.1:88388313-88388332 | MS.gene034241:CDS | 50.0% | |
| ! | CACTCCAAGCATTGTCACTG+TGG | - | chr4.1:88387607-88387626 | None:intergenic | 50.0% |
| ! | CTCCAAGCATTGTCACTGTG+GGG | - | chr4.1:88387605-88387624 | None:intergenic | 50.0% |
| !! | AACTTGTTGGCTGGTACGAC+TGG | + | chr4.1:88388302-88388321 | MS.gene034241:CDS | 50.0% |
| !! | ACCTCTCACAACTTGTTGGC+TGG | + | chr4.1:88388293-88388312 | MS.gene034241:CDS | 50.0% |
| !! | GATCTGACTGGATTGGCTAG+AGG | + | chr4.1:88387936-88387955 | MS.gene034241:CDS | 50.0% |
| !! | TGATTACAGTGCTGACGGTG+AGG | + | chr4.1:88387902-88387921 | MS.gene034241:CDS | 50.0% |
| ATTGGCTAGAGGTGGTCAGC+AGG | + | chr4.1:88387947-88387966 | MS.gene034241:CDS | 55.0% | |
| GTCCCCACAGTGACAATGCT+TGG | + | chr4.1:88387600-88387619 | MS.gene034241:CDS | 55.0% | |
| !! | CTGACTGGATTGGCTAGAGG+TGG | + | chr4.1:88387939-88387958 | MS.gene034241:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 88387570 | 88388349 | 88387570 | ID=MS.gene034241 |
| chr4.1 | mRNA | 88387570 | 88388349 | 88387570 | ID=MS.gene034241.t1;Parent=MS.gene034241 |
| chr4.1 | exon | 88387570 | 88388349 | 88387570 | ID=MS.gene034241.t1.exon1;Parent=MS.gene034241.t1 |
| chr4.1 | CDS | 88387570 | 88388349 | 88387570 | ID=cds.MS.gene034241.t1;Parent=MS.gene034241.t1 |
| Gene Sequence |
| Protein sequence |
